Journal of Equine Veterinary Science最新文献_第6页

Genetic parameter estimates of performance traits in Iranian Thoroughbred race horses using a Bayesian approach 用贝叶斯方法估计伊朗纯种马性能性状的遗传参数。

IF 1.6 3区农林科学 Q2 VETERINARY SCIENCES

Journal of Equine Veterinary Science

Pub Date : 2025-11-14 DOI: 10.1016/j.jevs.2025.105733

M. Taned , M.B. Zandi , S. Salek Ardestani , M. Abdoli , M. L Spangler

Background

Race performance in Thoroughbred horses is influenced by both genetic and environmental factors. Understanding their genetic architecture is essential for designing effective breeding programs.

Objectives

This study aimed to estimate genetic parameters and trends for key performance traits in Iranian Thoroughbred racehorses.

Methods

Performance data, including race completion time (RCT), rank at the end of the competition (REC), and average speed (AS), were obtained from the Iranian Equestrian Federation (2002–2021). Pedigree data from 14,391 individuals were analyzed using CFC software. Genetic parameters were estimated using a Bayesian approach via Gibbs sampling with gibbs1f90 for continuous traits (RCT and AS) and thrgibbs1f90 for the categorical trait (REC).

Results

Heritability estimates were moderate for RCT (0.25) and AS (0.23), and low for REC (0.09). Rider and date/race city effects contributed notably to variation in RCT and AS. Genetic trend analysis showed an unfavorable increase in RCT (0.15 s/year; p = 0.02) and a significant decline in AS (−0.02 m/s/year; p = 0.0001), while the genetic trend for REC was negligible (−0.0004; p = 0.94). Phenotypic trends did not follow the same direction, suggesting strong environmental influences masking genetic changes.

Conclusion

RCT and AS showed moderate heritability, indicating potential for genetic selection, whereas REC was mainly influenced by environmental factors. Despite stable phenotypic performance, unfavorable genetic trends highlight the need to revise breeding strategies and enhance long-term genetic improvement in Iranian Thoroughbred racehorses.

背景：纯种马的比赛表现受到遗传和环境因素的双重影响。了解它们的遗传结构对于设计有效的育种计划至关重要。目的：本研究旨在估计伊朗纯种马主要性能性状的遗传参数和趋势。方法：从伊朗马术联合会（2002-2021）获得成绩数据，包括比赛完成时间（RCT）、比赛结束排名（REC）和平均速度（AS）。使用CFC软件分析14,391人的家谱数据。采用贝叶斯方法对连续性状（RCT和AS）和分类性状（REC）进行Gibbs抽样，用gibbs1f90估计遗传参数。结果：RCT（0.25）和AS（0.23）的遗传力估计中等，REC（0.09）的遗传力估计较低。骑手和日期/种族城市效应对RCT和AS的差异有显著影响。遗传趋势分析显示，RCT增加不利（0.15 s/年，p = 0.02），AS显著下降（-0.02 m/s/年，p = 0.0001），而REC的遗传趋势可以忽略不计（-0.0004,p = 0.94）。表型趋势并没有遵循相同的方向，这表明强烈的环境影响掩盖了遗传变化。结论：RCT和AS具有中等遗传力，具有遗传选择的潜力，而REC主要受环境因素的影响。尽管表型表现稳定，但不利的遗传趋势突出了修改育种策略和加强伊朗纯种马长期遗传改良的必要性。

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引用次数: 0

Rapid and sensitive detection of Theileria equi using a novel integrated RPACRISPR/Cas13a lateral flow assay 利用新型集成RPACRISPR/Cas13a横向流动试验快速灵敏地检测马伊勒氏菌

IF 1.6 3区农林科学 Q2 VETERINARY SCIENCES

Journal of Equine Veterinary Science

Pub Date : 2025-11-10 DOI: 10.1016/j.jevs.2025.105732

Amjed Alsultan , Salah Mahdi Karim , Mohammed Al-Saadi , Dhama Alsallami , Mourad Ben Said , Hanène Belkahia

Background

Equine piroplasmosis (EP), caused by the intracellular protozoa Theileria equi, Babesia caballi, and Theileria haneyi, represents a major health and economic threat to the equine industry worldwide. Existing diagnostic methods, including PCR, serology, and microscopy, are constrained by their dependence on specialized equipment, lengthy protocols, and the requirement for skilled personnel.

Aim

This study aimed to develop a rapid, accurate, and field-deployable molecular diagnostic assay for T. equi.

Methods

A nucleic acid-based diagnostic platform combining recombinase polymerase amplification (RPA) with CRISPR/Cas13-mediated detection and lateral flow device (LFD) readout was developed. The assay targets a conserved region of the erythrocyte merozoite antigen 1 (EMA-1) gene of T. equi. Validation was performed using 22 blood samples collected from horses, as well as specificity controls including B. caballi- and Anaplasma phagocytophilum-infected samples, synthetic EMA-1 DNA, and non-template controls. All assay steps were conducted at room temperature.

Results

The integrated RPA-CRISPR/Cas13-LFD assay generated clear visual results within 50 minutes. It demonstrated complete specificity with no false positives across all tested samples. The method effectively differentiated horses infected with T. equi, including both clinically affected and asymptomatic individuals, from healthy, uninfected animals, confirming its high accuracy and reliability.

Conclusion

The developed assay provides a rapid, precise, and equipment-free diagnostic platform suitable for both field and clinical environments. Although the current protocol relies on DNA extraction, future optimization will aim to enable direct detection from unprocessed blood samples, thereby further simplifying point-of-care diagnostics for equine piroplasmosis.

背景：马螺浆虫病（EP）是由细胞内原生动物马伊勒氏菌、卡巴贝斯虫和哈尼伊伊勒氏菌引起的，是全球马业的主要健康和经济威胁。现有的诊断方法，包括聚合酶链反应（PCR）、血清学和显微镜，由于依赖专门的设备、冗长的协议和对熟练人员的要求而受到限制。目的：本研究旨在建立一种快速、准确、可在现场部署的马蹄疫分子诊断方法。方法：建立结合重组酶聚合酶扩增（RPA）、CRISPR/ cas13介导检测和侧流装置（LFD）读数的核酸诊断平台。该检测的目标是马蹄疫红细胞裂殖子抗原1 （EMA-1）基因的一个保守区域。使用从马身上采集的22份血液样本进行验证，以及特异性对照，包括caballi和无原体吞噬细胞感染样本、合成EMA-1 DNA和非模板对照。所有实验步骤均在室温下进行。结果：整合的RPA-CRISPR/Cas13-LFD检测在50分钟内产生了清晰的视觉结果。它显示出完全的特异性，在所有测试样本中没有假阳性。该方法有效地将感染马弓形虫的马匹（包括临床感染和无症状个体）与未感染的健康动物区分开来，证实了其较高的准确性和可靠性。结论：开发的检测方法提供了一个快速、精确、无设备的诊断平台，适用于现场和临床环境。虽然目前的方案依赖于DNA提取，但未来的优化将旨在实现从未处理的血液样本中直接检测，从而进一步简化马体浆虫病的即时诊断。

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引用次数: 0

Proof of concept of a wearable IoT-based system for monitoring respiratory rate and surface temperature in horses 基于物联网的可穿戴系统的概念验证，用于监测马的呼吸频率和表面温度。

IF 1.6 3区农林科学 Q2 VETERINARY SCIENCES

Journal of Equine Veterinary Science

Pub Date : 2025-11-08 DOI: 10.1016/j.jevs.2025.105729

Brendo Junior Pereira Farias , Dermeval Araújo Furtado , José Wallace Barbosa do Nascimento , José Pinheiro Lopes Neto , Fabiana Terezinha Leal de Morais , Thiago Lira Souza Santos , Alexandre Sales Vasconcelos , Rafael Costa Silva , José Ilton Pereira Alves , Concepta Mcmanus , Robson Mateus Freitas Silveira , Neila Lidiany Ribeiro

This study presents the development and proof of concept of a wearable monitoring system designed to measure respiratory rate (RR) and coat surface temperature (CST) in horses. The device integrates an embedded system based on an ESP-32 microcontroller equipped with sensors for RR (strain gauge) and CST (DS18B20) Data are transmitted in real time via Wi-Fi and stored locally on SD cards. The system was validated against conventional manual methods under field conditions. Agreement between methods was evaluated using Intraclass Correlation Coefficients (ICC) and Bland-Altman plots. Results showed excellent reproducibility for RR (ICC = 0.85) and very good reproducibility for CST (ICC = 0.67), with no significant differences detected by paired t-tests (p > 0.05). The Bland-Altman analysis indicated low bias and narrow limits of agreement for both variables. This proof-of-concept demonstrates that the wearable system provides reliable, accurate, and non-invasive measurements of key physiological and environmental parameters in horses.

本研究介绍了一种可穿戴监测系统的开发和概念验证，该系统旨在测量马的呼吸速率（RR）和皮毛表面温度（CST）。该设备集成了一个基于ESP-32微控制器的嵌入式系统，配备了RR（应变计）和CST （DS18B20）传感器，数据通过Wi-Fi实时传输，并存储在本地SD卡上。该系统在现场条件下与传统的人工方法进行了验证。采用类内相关系数（ICC）和Bland-Altman图评价方法间的一致性。结果显示，RR的重复性好（ICC = 0.85），CST的重复性好（ICC = 0.67），配对t检验无显著差异（p > 0.05）。Bland-Altman分析表明，这两个变量的偏倚较低，一致性限制较窄。这一概念验证表明，可穿戴系统为马的关键生理和环境参数提供了可靠、准确和非侵入性的测量。

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引用次数: 0

L-Carnitine supplementation to the stallion semen extender attenuates the cryopreservation induced oxidative stress and ameliorates sperm functional attributes 在种马精液扩展剂中添加左旋肉碱可减轻低温保存引起的氧化应激，改善精子功能属性。

IF 1.6 3区农林科学 Q2 VETERINARY SCIENCES

Journal of Equine Veterinary Science

Pub Date : 2025-11-08 DOI: 10.1016/j.jevs.2025.105731

Omprakash Solanki , Thirumala Rao Talluri , Dinesh Jhamb , Sajjan Kumar , Kalpesh Kumar Pargi , Nilendu Paul , M Kutty , RK Dedar , Madhu Meena , Mitesh Gaur , TK Bhattacharya

Objective

The current study evaluated the effect of L-Carnitine supplementation to the stallion semen extender on pre-freeze and post-thaw semen quality and oxidative parameters in Marwari breed stallions.

Materials and Methods

For this purpose, thirty-six semen ejaculates (6 ejaculates from each stallion) were collected using an artificial vagina (AV) from six adult Marwari stallions. The gel free semen was extended with different concentrations of L-Carnitine (LC) i.e. 0 mM (control), 2.5 mM (T1), 5 mM (T2) and 10 mM (T3). Pre-freeze and post-thaw semen evaluations were conducted for various seminal attributes and oxidative parameters.

Results

The results indicated that, all LC supplemented groups (2.5, 5 and 10 mM) exhibited improved progressive motility, acrosome integrity, plasma membrane integrity and mitochondrial membrane potential (MMP) at both pre-freeze and post -thaw stages (P < 0.05) in comparison to the control group. Compared to that in the control, the addition of 5 mM LC induced an increase (P < 0.01) in sperm motility (31.76 ± 2.08 vs 43.87 ± 1.95 %), acrosome integrity (58.56 ± 0.88 vs 66.86 ± 1.11) and MMP (55.66 ± 0.84 vs 65.55 ± 1.15). Antioxidant enzyme levels viz, superoxide dismutase (SOD) and total antioxidant capacity (T-AOC), levels were increased (P < 0.01), glutathione peroxidase (GPX) levels also showed higher values (P < 0.05), exclusively in 5 mM (T2) group and malondialdehyde (MDA) levels decreased (P < 0.05) in the all the LC supplemented groups at post-thaw stage.

Conclusion

In conclusion, L-Carnitine supplementation to the stallion semen extender enhanced the semen quality at pre- and post-thaw stages and at 5 mM LC, the oxidative parameters were ameliorated in post- thaw semen of Marwari breed stallions.

目的：研究马精液膨化剂中添加左旋肉碱对马尔瓦里种马冷冻前、解冻后精液质量和氧化参数的影响。材料和方法：采用人工阴道（AV）采集了6匹马尔瓦里成年马的36枚射精（每匹6枚射精）。用不同浓度的左旋肉碱（LC）延长无凝胶精液，分别为0mM（对照）、2.5mM （T1）、5mM （T2）和10mM （T3）。对冷冻前和解冻后的精液进行了各种精液属性和氧化参数的评价。结果：所有LC添加组（2.5、5和10mM）在冷冻前和解冻后均表现出改善的渐进式运动性、顶体完整性、质膜完整性和线粒体膜电位（MMP） (p)。综上所述，马精液膨化剂中添加左旋肉碱提高了马解冻前和解冻后的精液质量，并在5 mM LC下改善了马解冻后精液的氧化参数。

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引用次数: 0

Readers Information 读者信息

IF 1.6 3区农林科学 Q2 VETERINARY SCIENCES

Journal of Equine Veterinary Science

Pub Date : 2025-11-01 DOI: 10.1016/S0737-0806(25)00378-8

引用次数: 0

Treatment efficacy of pyrantel, fenbendazole and macrocyclic lactones in equine strongyles in Germany using FECRT and the model eggCounts 采用FECRT和模型卵计数法观察吡喃酮、芬苯达唑和大环内酯对德国马圆形细胞的治疗效果。

IF 1.6 3区农林科学 Q2 VETERINARY SCIENCES

Journal of Equine Veterinary Science

Pub Date : 2025-10-31 DOI: 10.1016/j.jevs.2025.105715

J. Döberl , Z. Li , R. Furrer , M.C. Jäger

Background

Reduced anthelmintic efficacy against small strongyles in horses is a widespread problem, yet recent data is scarce.

Aims

This paper aims to investigate the current status of anthelmintic efficacy in the field in Germany, specifically focusing on pyrantel.

Methods

The study included 1670 horses for which a total of 1913 dewormings were analysed. Of these, 1682 were performed with pyrantel, 56 with fenbendazole, 162 with macrocyclic lactones and 13 with other compounds (e.g. herbs). The faecal egg count reduction (FECR) was calculated with two methods, the faecal egg count reduction test (FECRT) and the statistical model eggCounts. In interpreting the results, we adhered to the guidelines of the American Association of Equine Practitioners (AAEP) and the World Association for the Advancement of Veterinary Parasitology (WAAVP).

Results

Overall, 1156 dewormings (68.7 %) with pyrantel, 12 (21.4 %) with fenbendazole and 159 (98.1 %) with macrocyclic lactones were scored with a FECR >95 %. Pyrantel is classified as ‘low resistant’ with both FECRT (UCL/LCL: 91.1/89.5) and eggCounts (88.7/88.1), fenbendazole as ‘resistant’ with FECRT (65.0/49.1) and eggCounts (50.4/42.9), and macrocyclic lactones as ‘susceptible’ with FECRT (100.1/99.0) and as ‘low resistant’ with eggCounts (99.8/99.5).

Conclusion

Whereas treatments with macrocyclic lactones were sufficiently effective, the majority of fenbendazole treatments showed insufficient efficacy. For pyrantel the results confirm reduced treatment efficacy in 31.3 % of the treatments. Nevertheless, most treatments remained effective. Consequently, pyrantel should be continued to be used for treating strongyle infections. A control of treatment efficacy 10–14 days after administration of the anthelmintic should become standard practice.

背景：对马的小圆线虫驱虫药效力降低是一个普遍存在的问题，但最近的数据很少。目的：本文旨在调查德国野外驱虫功效的现状，重点研究吡喃醚。方法：对1670匹马共进行了1913次驱虫分析。其中，1682例与吡喃酮有关，56例与芬苯达唑有关，162例与大环内酯有关，13例与其他化合物（如草药）有关。粪卵计数减少（FECR）采用粪卵计数减少试验（FECRT）和统计模型计数两种方法计算。在解释结果时，我们遵循了美国马术从业者协会（AAEP）和世界兽医寄生虫学发展协会（WAAVP）的指导方针。结果：吡喃醚驱虫1156只（68.7%），芬苯达唑驱虫12只（21.4%），大环内酯驱虫159只（98.1%）。吡喃酮被分类为“低耐药”，FECRT （UCL/LCL: 91.1/89.5）和鸡蛋计数（88.7/88.1），芬苯达唑被分类为“耐药”，FECRT（65.0/49.1）和鸡蛋计数（50.4/42.9），大环内酯被分类为“敏感”，FECRT（100.1/99.0）和鸡蛋计数（99.8/99.5）。结论：大环内酯类药物治疗效果良好，而非苯达唑类药物治疗效果不佳。对于吡喃酮，结果证实31.3%的治疗效果下降。然而，大多数治疗方法仍然有效。因此，吡喃嘧啶应继续用于治疗圆形细胞感染。使用驱虫药后10- 14天的治疗效果控制应成为标准做法。

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引用次数: 0

Radiographic measurements of Miniature Horse and Miniature Pony Feet 微型马和微型小马足的射线照相测量。

IF 1.6 3区农林科学 Q2 VETERINARY SCIENCES

Journal of Equine Veterinary Science

Pub Date : 2025-10-31 DOI: 10.1016/j.jevs.2025.105710

J․M Olijnyk , G. Hosgood , J. Richardson , C. Beggs , E. McConnell

Background

Reliable assessment of foot conformation is essential for podiatric management in Miniature Horses and Ponies, yet reference ranges and the reliability of radiographic measurements remain poorly defined.

Aims/objectives

This study quantified radiographic measurements of foot conformation in sound, skeletally mature Miniature Horses and Ponies and evaluated their agreement, reliability, and precision.

Methods

Standardised lateromedial and dorsopalmar/plantarodorsal radiographs were obtained from 30 Miniature Horses and Ponies. Two observers took 60 measurements from 10 sets of projections on three occasions. Reliability and precision were quantified using intraclass correlation coefficients (ICC) and within-subject coefficients of variation (CV), and agreement was assessed against ±5 % limits of agreement (LOA). These measurements were then obtained by a single observer from 30 horses. The median and 95 % confidence intervals (CI) of each measurement were calculated.

Results

Most linear and angular measurements had moderate to excellent reliability (median ICC 0.86, interquartile range [IQR] 0.22) and precision (median CV 4 %, IQR 7 %) but exceeded ±5 % LOA. Hoof wall thickness ratios had good reliability (minimum ICC 0.69, 95 % CI 0.49–0.96), excellent precision (maximum CV 9 %, 95 % CI 2–11 %), and acceptable agreement (maximum LOA ±4.7 %, 95 % CI −0.11–0.09). Marginal means (95 %CIs) were: dorsal, forefeet 0.245 (0.237–0.253), hindfeet 0.240 (0.232–0.248); lateral, forefeet 0.186 (0.180–0.194), hindfeet 0.180 (0.173–0.186); medial, pre-trim 0.177 (0.171–0.183), post-trim 0.169 (0.163-0.175).

Conclusion

Hoof wall ratios provide consistent, repeatable radiographic measurements suitable for clinical use in skeletally mature Miniature Horses and Ponies, supporting objective laminitis screening and monitoring.

背景：可靠的足部形态评估对微型马和矮种马的足部管理至关重要，但参考范围和x线测量的可靠性仍然不明确。目的/目的：本研究量化了健全、骨骼成熟的微型马和矮种马足部构象的射线测量，并评估了它们的一致性、可靠性和准确性。方法：对30匹小型马和矮种马的侧内侧和背跖骨/跖骨进行标准化x线片检查。两名观察员分三次对10组投影进行了60次测量。采用类内相关系数（ICC）和受试者内变异系数（CV）对可靠性和精密度进行量化，并根据±5%的一致性限（LOA）评估一致性。然后由一个观察者从30匹马中获得这些测量值。计算每次测量的中位数和95%置信区间（CI）。结果：大多数线性和角度测量具有中等至优异的信度（中位ICC 0.86，四分位间距[IQR] 0.22）和精密度（中位CV 4%， IQR 7%），但LOA超过±5%。蹄壁厚度比具有良好的可靠性（最小ICC 0.69, 95% CI 0.49-0.96），优良的精度（最大CV 9%， 95% CI 2-11%）和可接受的一致性（最大LOA±4.7%,95% CI -0.11-0.09）。边际均值（95% ci）为：背侧，前足0.245(0.237-0.253)，后足0.240 (0.232-0.248)；侧面，前脚0.186(0.180-0.194)，后脚0.180 (0.173-0.186)；内侧，预修剪0.177(0.171-0.183)，后修剪0.169（0.163-0.175）。结论：蹄壁比提供了一致的、可重复的x线测量，适用于骨骼成熟的微型马和小马的临床应用，支持客观的板层炎筛查和监测。

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引用次数: 0

Lethal Sycamore maple intoxication in a grazing three-month-old foal 吃草的三个月大的马驹死于枫树中毒。

IF 1.6 3区农林科学 Q2 VETERINARY SCIENCES

Journal of Equine Veterinary Science

Pub Date : 2025-10-30 DOI: 10.1016/j.jevs.2025.105713

N. Janzen , J. Sander , M. Terhardt , M. Mallek , H Smith , P. Witt , M.J.P. Theelen

The case of a young foal poisoned by maple toxin is described. It showed signs of myopathy caused by ingesting life-threatening amounts of maple toxin. Some maple trees (Acer species) can pose a health risk to grazing horses. The leaves, seeds and shoots contain hypoglycin A (HGA) and methylencyclopropylglycine (MCPRG) and can cause life-threatening atypical myopathy.

一个年轻的马驹中毒的枫毒素的情况下描述。它表现出因摄入危及生命的枫毒素而引起的肌病的迹象。一些枫树（槭属）会对吃草的马造成健康风险。叶子、种子和芽含有次甘氨酸A （HGA）和甲基丙基甘氨酸（MCPRG），可引起危及生命的非典型肌病。

引用次数: 0

Policresulen is effective for the topical treatment of vaginal varices in a pregnant mare: Case report Policresulen是有效的局部治疗阴道静脉曲张妊娠母马：病例报告。

IF 1.6 3区农林科学 Q2 VETERINARY SCIENCES

Journal of Equine Veterinary Science

Pub Date : 2025-10-30 DOI: 10.1016/j.jevs.2025.105711

Ellen Lara Miguel , Maria Fernanda Lema Carneiro , Ana Luiza Carriel Griffo , Fernanda Baatsch Nascimento , Renata Gebara Sampaio Dória , Rodrigo Romero Corrêa , Eneiva Carla Carvalho Celeghini

Vaginal varicosities in mares are rarely reported, although considered relatively common. Clinical recognition can be challenging, especially during early pregnancy, due to the intermittent nature of the bleeding. This report aims to describe the diagnostic approach, clinical evolution, and treatment of a case of vaginal varicosity in a pregnant mare, emphasizing a practical and minimally invasive method. A 9-year-old Brazilian Sport Horse mare with intermittent vaginal hemorrhage underwent clinical evaluation, showing no abnormalities. Vaginoscopy with a rigid endoscope revealed varicose veins in the vestibule-vaginal region. Chemical cauterization was performed using a 36 % Policresulen solution. The treatment was well tolerated, inexpensive, and effective in controlling bleeding. The clinical sign resolved within a few days, with no recurrence observed. Rigid endoscopy proved to be a practical diagnostic tool for field use. Policresulen offered an efficient, low-cost, and minimally invasive therapeutic option for managing vaginal varicosities in mares.

母马阴道静脉曲张虽然被认为相对常见，但很少有报道。由于出血的间歇性，临床识别可能具有挑战性，特别是在妊娠早期。本报告旨在描述一例妊娠母马阴道静脉曲张的诊断方法、临床进展和治疗，强调一种实用的微创方法。一个9岁的巴西运动马母马间歇性阴道出血接受临床评估，显示没有异常。阴道镜与刚性内窥镜显示静脉曲张在前庭-阴道区域。使用36%的Policresulen溶液进行化学烧灼。该疗法耐受性好，价格低廉，并能有效控制出血。临床症状在几天内消失，无复发。刚性内窥镜被证明是一种实用的现场诊断工具。Policresulen为治疗母马阴道静脉曲张提供了一种高效、低成本、微创的治疗选择。

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引用次数: 0

Seasonal assessment of welfare and Endoparasitic infections in Balkan donkeys 巴尔干驴福利和内寄生虫感染的季节性评估。

IF 1.6 3区农林科学 Q2 VETERINARY SCIENCES

Journal of Equine Veterinary Science

Pub Date : 2025-10-30 DOI: 10.1016/j.jevs.2025.105714

Marko Nadaškić , Tamara Ilić , Nemanja M. Jovanović , Tamaš Petrović , Dejan Bugarski , Jelena Aleksić Radojković , Marijana Vučinić , Katarina Nenadović

Background

In Serbia, donkey breeding is meeting a growing trend, primarily for milk production. However, the welfare status of donkeys kept under such farming systems remains inadequately evaluated, with limited data available on their health, behavior, and overall management conditions.

Aims

This study aimed to evaluate welfare indicators in donkeys, determine the prevalence of endoparasitic infections, and assess their potential effects on the animals’ welfare status.

Methods

The study was conducted during spring and autumn season, on a sample of 160 Balkan donkeys aged 1 to 15 years from five selected farms. Welfare was assessed using the “Animal Welfare Indicators” welfare protocol for donkeys, while parasitological diagnostics were performed on fecal samples.

Results

In donkeys in spring, the mean body condition score, skin lesions, unhealthy hair coat condition, ocular discharges, and signs of hoof neglect were significantly higher than those for donkeys in autumn(p < 0.001, p < 0.05). The mean score of skin tent test, and faecal soiling in autumn were significantly higher than those for donkeys in spring (p < 0.001, p < 0.05). Parasites identified included Eimeria leuckarti, strongylids, Parascaris equorum, Dictyocaulus arnfieldi, Anoplocephala spp., Fasciola hepatica and Dicrocoelium dendriticum in the form of individual and mixed infections. Significant correlations (p < 0.001, p < 0.05) were found between P. equorum and faecal soiling, strongylids and thin BCS in spring, and strongylids and loss of hair in autumn season.

Conclusions

This study emphasizes the association between parasitic infections and donkey welfare, underscoring the need for regular monitoring and the development of guidelines to improve health and welfare protection in Serbia.

背景：在塞尔维亚，驴养殖正处于增长趋势，主要是为了生产牛奶。然而，在这种农业系统下饲养的驴的福利状况仍然没有得到充分的评估，关于它们的健康、行为和总体管理条件的数据有限。目的：评价毛驴福利指标，确定毛驴体内寄生虫感染的流行情况，并评估其对毛驴福利状况的潜在影响。方法：研究在春季和秋季进行，选取了来自5个农场的160头1至15岁的巴尔干驴作为样本。使用驴的“动物福利指标”福利方案评估福利，同时对粪便样本进行寄生虫学诊断。结果：春季驴的平均身体状况评分、皮肤病变、不健康的毛被状况、眼分泌物和蹄部忽视的迹象明显高于秋季驴。结论：本研究强调了寄生虫感染与驴福利之间的联系，强调了定期监测和制定指导方针的必要性，以改善塞尔维亚的健康和福利保护。

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引用次数: 0