Journal of Equine Veterinary Science最新文献_第9页

Mutation rate and spectrum of germline de novo mutations in a closed population of Thoroughbred horses 纯种马封闭种群种系新生突变的突变率和谱

IF 1.6 3区农林科学 Q2 VETERINARY SCIENCES

Journal of Equine Veterinary Science

Pub Date : 2025-09-01 DOI: 10.1016/j.jevs.2025.105682

Risako Furukawa , Teruaki Tozaki , Keijiro Mizukami , Yusuke Iwasaki , Koki Kawate , Mio Kikuchi , Taichiro Ishige , Yukihide Momozawa , Emiko Fukui , Hironaga Kakoi

Background

Thoroughbreds have been maintained as a closed breed for over 300 years since the crossbreeding between Arabian stallions and English mares. Despite interest in germline de novo mutations across species, their frequency in horses, particularly in Thoroughbreds, remains largely unexplored.

Aims/objective

This study aimed to identify de novo mutations in Thoroughbreds and estimate their frequency within a genetically closed population.

Methods

We performed deep whole-genome sequencing (≥230× depth, 150 bp paired-end reads) and Sanger validation in a Thoroughbred trio (sire, dam, and foal). Reads were aligned to the EquCab3.0 reference genome, and variants in regions with sequencing depth ≥20 (covering 99.6 % of the genome) were analyzed for Mendelian inconsistencies.

Results

A total of 48 de novo mutations were identified, comprising 46 single nucleotide substitutions and 2 deletions, none of which were found in public variant databases. Of these, 18 were of paternal origin and 6 were of maternal origin (P < 0.05). The estimated mutation rate was 9.59 × 10⁻⁹, which is in a comparable range to that reported in humans. One mutation (chr7:g.2084761G>A) introduced a nonsense variant in the zinc finger protein 77 gene, which encodes a putative transcriptional regulator.

Conclusions

De novo mutations were identified in the Thoroughbred genome, and the mutation rate is consistent with estimates from other mammalian studies. A significant bias toward paternal origin was observed. Our findings suggest that germline de novo mutations are a source of novel genetic variation in Thoroughbreds, even within a genetically closed population.

自阿拉伯种马和英国母马杂交以来，纯种马作为一个封闭品种已经维持了300多年。尽管人们对物种间的生殖系新生突变很感兴趣，但它们在马，尤其是纯种马中的频率，在很大程度上仍未被探索。目的/目的本研究旨在鉴定纯种马的新生突变，并估计其在遗传封闭群体中的频率。方法对三头纯种马（公马、公马和马驹）进行了深度全基因组测序（≥230×深度，150 bp对端reads）和Sanger验证。Reads与EquCab3.0参考基因组对齐，测序深度≥20（覆盖99.6%的基因组）区域的变异进行孟德尔不一致性分析。结果共鉴定出48个新生突变，包括46个单核苷酸替换和2个缺失，这些突变均未在公共变异数据库中发现。其中父系18例，母系6例（P < 0.05）。估计的突变率为9.59 × 10（毒血症），与人类报告的范围相当。一个突变（chr7:g.2084761G>；A）在锌指蛋白77基因中引入了一个无义变异，该基因编码一个假定的转录调节因子。结论在纯种马基因组中发现了新生突变，突变率与其他哺乳动物研究的估计一致。观察到对父系出身的显著偏见。我们的研究结果表明，即使在遗传封闭的种群中，种系新生突变也是纯种马新遗传变异的来源。

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引用次数: 0

How prior racing performance influences competition level in off-the-track Thoroughbred horses’ post-racing eventing careers 在赛道外纯种马的赛事生涯中，先前的比赛表现如何影响比赛水平

IF 1.6 3区农林科学 Q2 VETERINARY SCIENCES

Journal of Equine Veterinary Science

Pub Date : 2025-08-30 DOI: 10.1016/j.jevs.2025.105679

Elouise K Bacon, Danica Parnell, Gary Muscatello, Jaimie McElroy, Brandon D Velie

Background

The ongoing discussion surrounding the well-being of retired racehorses underscores the importance of implementing effective rehoming strategies that prioritise animal welfare. A significant knowledge gap persists regarding whether a horse’s success in racing can be used to reliably predict its performance in different equestrian sports upon retirement from racing.

Aims/Objectives

To examine the association between racehorse performance metrics and eventing metrics in off-the-track Thoroughbreds that compete in eventing within Australia.

Methods

Racing metrics and eventing metrics for a sample of off-the-track Thoroughbreds (n = 6,828) registered with Equestrian Australia were collated. Summary statistics of racing metrics were estimated and assessed for differences between horses competing in eventing at level EvA95 and above versus horses competing at levels below EvA95. Generalised linear models were applied to assess the influence of racing performance metrics on eventing performance measures at levels EvA95 and above.

Results

Horses that failed to reach an eventing level of EvA95 were shown to have had significantly (P < 0.01) better racing performance and longer racing careers. Time elapsed since last race was shown to be favourably associated with most eventing parameters assessed and longer best race distances were shown to be significantly associated with higher levels of eventing achieved.

Conclusions

Riders seeking high-level eventing prospects may want to prioritize Thoroughbreds that excelled at longer race distances; however, racing performance alone does not appear to be a reliable predictor of success in eventing. Other factors, such as stride characteristics and the suitability of the rider-horse partnership, also warrant thoughtful consideration.

围绕退役赛马福利的持续讨论强调了实施优先考虑动物福利的有效的重新安置策略的重要性。一匹马在比赛中的成功是否可以用来可靠地预测其退役后在不同马术运动中的表现，这方面的知识鸿沟仍然存在。目的/目的研究在澳大利亚参加三项赛的纯种马中，赛马表现指标与三项赛指标之间的关系。方法对在澳大利亚马术协会注册的脱场纯种马样本（n = 6,828）的竞赛指标和三项指标进行整理。估计和评估EvA95及以上水平的赛马与EvA95以下水平的赛马在三项赛中的差异。应用广义线性模型来评估EvA95及以上水平的赛车成绩指标对三项赛成绩指标的影响。结果EvA95水平未达到三项赛水平的马，其比赛成绩显著提高（P < 0.01），比赛生涯延长。从上次比赛到现在的时间被证明与大多数项目参数有良好的联系，而较长的最佳比赛距离被证明与较高的项目水平有显著的联系。寻求高水平赛事前景的骑手可能会优先考虑在长距离比赛中表现出色的纯种马；然而，单凭赛车成绩似乎并不能可靠地预测在三项赛中取得成功。其他因素，如步幅特征和骑手与马匹合作的适宜性，也值得深思。

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引用次数: 0

Effects of mixed polyphenol supplementation on lower airway inflammation in horses with Equine Asthma Syndrome 混合多酚对马哮喘综合征马下气道炎症的影响。

IF 1.6 3区农林科学 Q2 VETERINARY SCIENCES

Journal of Equine Veterinary Science

Pub Date : 2025-08-30 DOI: 10.1016/j.jevs.2025.105681

K.M. Andrews, L.J. Berghaus, K.A. Hart

Background

Equine Asthma Syndrome (EAS) is a chronic inflammatory respiratory disease of adult horses. While dietary polyphenols have shown anti-inflammatory effects in human asthma, their use in equine asthma has not been studied.

Aims

To evaluate the effects of a mixed polyphenol supplement on systemic and pulmonary inflammation, bronchoalveolar lavage (BAL) cytology, and clinical respiratory signs in healthy and asthmatic horses. We hypothesized that polyphenol supplementation would decrease systemic and pulmonary inflammation in both healthy and asthmatic horses and would improve BAL cytology and clinical respiratory signs in asthmatic horses.

Methods

In Part One, 18 horses (eight healthy, 10 asthmatic) received a daily mixed polyphenol supplement for 6–8 weeks. Physical examination, BAL cytology, and cytokine concentrations in serum and BAL fluid were assessed before and after supplementation. In Part Two, 10 asthmatic horses were randomized into supplemented and control groups (n = 5/group). After 4 weeks on a low-dust diet with or without supplementation, airway inflammation was exacerbated using dusty hay for 4 weeks. Clinical respiratory signs, rebreathing exam findings, and pulmonary inflammation were assessed.

Results

In asthmatic horses in Part One, BAL % neutrophils and BAL IL-6 concentrations significantly decreased post-supplementation (P = 0.02; 95 % CI = 93.86 to 913.2 pg/ml), and a greater decrease in BAL IL-10 was observed in asthmatic compared to healthy horses (P = 0.02; 95 % CI = -22.4 to -0.01). In Part Two, the proportion of horses with abnormal rebreathing exams differed significantly between groups (P = 0.002; 95 % CI = 0.11 to 0.41), and supplemented horses had significantly lower respiratory rates (P = 0.008; 95 % CI = -18.0 to -4.0).

Conclusions

Polyphenol supplementation may modulate pulmonary inflammation and improve clinical signs in mildly asthmatic horses when combined with environmental management.

背景：马哮喘综合征（EAS）是成年马的一种慢性炎症性呼吸道疾病。虽然膳食多酚在人类哮喘中显示出抗炎作用，但它们在马哮喘中的应用尚未得到研究。目的：评价混合多酚补充剂对健康和哮喘马的全身和肺部炎症、支气管肺泡灌洗（BAL）细胞学和临床呼吸体征的影响。我们假设补充多酚可以减少健康和哮喘马的全身和肺部炎症，并改善哮喘马的BAL细胞学和临床呼吸体征。方法：在第一部分，18匹马（8匹健康马，10匹哮喘马）每天服用混合多酚补充剂，持续6-8周。补充前后进行体格检查、BAL细胞学检查以及血清和BAL液中细胞因子浓度的评估。第二部分，将10匹哮喘马随机分为补充组和对照组（n = 5匹/组）。在低粉尘饮食（含或不含补充）4周后，使用含灰尘干草4周，气道炎症加剧。评估临床呼吸体征、再呼吸检查结果和肺部炎症。结果：在第一部分的哮喘马中，BAL %中性粒细胞和BAL IL-6浓度在补充后显著降低（P = 0.02;95% CI = 93.86至913.2 pg/ml），并且与健康马相比，哮喘马的BAL IL-10降低幅度更大（P = 0.02;95% CI = -22.4至-0.01）。在第二部分中，再呼吸检查异常的马的比例在各组之间有显著差异（P = 0.002;95% CI = 0.11 ~ 0.41），补充马的呼吸率显著降低（P = 0.008;95% CI = -18.0 ~ -4.0）。结论：与环境管理相结合，多酚补充可调节轻度哮喘马的肺部炎症并改善临床症状。

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引用次数: 0

Fistulous withers causing spinal epidural abscess in a Thoroughbred mare 在一匹纯种母马中引起硬膜外脓肿的瘘管性萎缩。

IF 1.6 3区农林科学 Q2 VETERINARY SCIENCES

Journal of Equine Veterinary Science

Pub Date : 2025-08-30 DOI: 10.1016/j.jevs.2025.105680

Serena Ceriotti , Stuart Clark-Price , Robert Cole , Alana Kramer , Maninder Sandey , Mariano Mora

This case describes a 15-year-old Thoroughbred mare with fistulous withers that progressed to vertebral osteomyelitis and spinal epidural abscess (SEA), a severe complication that has yet to be reported. Initially treated with antibiotics and anti-inflammatory medication, the mare developed acute hindlimb ataxia and spastic paresis, suggesting spinal cord compression at T3-L3. Diagnostic imaging revealed vertebral fractures and abscessation, but vertebral canal involvement could not be confirmed. Despite aggressive medical therapy, including antimicrobials, corticosteroids, and neuroprotective agents, the mare’s neurological condition deteriorated, leading to euthanasia. Post-mortem findings confirmed an extensive paraspinal abscess that had extended into the epidural space, causing severe spinal cord compression. The isolated pathogen, Escherichia coli, is commonly associated with chronic fistulous withers. This case underscores the importance of considering SEA as a diagnostic differential in horses with chronic paraspinal infections that develop acute neurological decline. Due to diagnostic and treatment limitations in equine medicine, prognosis remains guarded, with early surgical intervention and targeted antimicrobial therapy likely being crucial for better outcomes.

本病例描述了一只15岁的纯种母马，其瘘管性退马发展为椎体骨髓炎和脊髓硬膜外脓肿（SEA），这是一种严重的并发症，目前尚未有报道。最初给予抗生素和抗炎药物治疗，母马出现急性后肢共济失调和痉挛性轻瘫，提示T3-L3脊髓受压。诊断影像显示椎体骨折和脓肿，但椎管受累无法证实。尽管进行了积极的药物治疗，包括抗菌剂、皮质类固醇和神经保护剂，但母马的神经系统状况恶化，最终导致安乐死。尸检结果证实了一个广泛的椎管旁脓肿，已经延伸到硬膜外间隙，造成严重的脊髓压迫。分离的病原体，大肠杆菌，通常与慢性瘘管性萎凋有关。该病例强调了将SEA作为诊断慢性棘旁感染并发急性神经功能衰退的马的鉴别指标的重要性。由于马医学的诊断和治疗限制，预后仍然是谨慎的，早期手术干预和靶向抗菌治疗可能是获得更好结果的关键。

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引用次数: 0

Cytoplasmic and nuclear Survivin expression in healthy gastric mucosae of equids: A comparative study between horses, donkeys, and mules 马、驴和骡胃粘膜细胞浆和核Survivin表达的比较研究

IF 1.6 3区农林科学 Q2 VETERINARY SCIENCES

Journal of Equine Veterinary Science

Pub Date : 2025-08-18 DOI: 10.1016/j.jevs.2025.105678

A.L. Medina-Bolívar , J.D. Muñoz-Duque , J.R. Martínez-Aranzales

Background

Information regarding Survivin protein expression in the gastric mucosa of equids is scarce. This protein has been associated with functions related to modulating apoptosis and promoting mitosis in epithelial cells and is considered part of gastric cytoprotection mechanisms in humans and mice, maintaining mucosal integrity and regulating cellular renewal.

Aims/objectives

This study aimed to determine the expression of cytoplasmic and nuclear Survivin in gastric mucosae of equids (horses, donkeys, and mules).

Methods

A total of 30 healthy stomachs, 10 from each species, were evaluated using histology and immunohistochemistry techniques. Survivin protein was detected with antibody EP119 and with the polymer method.

Results

Immunohistochemistry revealed survivin expression in the gastric mucosa of equids. In horses and mules, the survivin index was evident in the cytoplasm of both glandular (59 ± 55; 55 ± 40) and squamous (15 ± 16; 18 ± 19) mucosa, being significantly higher in the glandular mucosa (P = 0.001; P = 0.041), respectively. In contrast, donkeys showed a higher survivin index in the cytoplasm, but no significant difference was observed between the two types of mucosa (P = 0.506).

Conclusion

Survivin expression in the healthy gastric tissue of equids was localized to the lamina propria and the basal layer of the epithelial layer, predominantly within the cytoplasmic compartment of the cells. However, further techniques are needed to study this protein in Equine Gastric Ulcer Syndrome (EGUS) to assess its potential role in cytoprotection and gastro-adaptation in these species.

背景关于马科动物胃粘膜Survivin蛋白表达的信息很少。该蛋白与调节上皮细胞凋亡和促进有丝分裂的功能有关，被认为是人类和小鼠胃细胞保护机制的一部分，维持粘膜完整性和调节细胞更新。目的研究马、驴、骡等马科动物胃粘膜细胞浆和核Survivin的表达。方法采用组织和免疫组化技术对30只健康胃进行组织学和免疫组化评价。用抗体EP119和聚合物法检测Survivin蛋白。结果免疫组化结果显示，马胃黏膜有survivin的表达。在马和骡子中，腺粘膜（59±55;55±40）和鳞状粘膜（15±16;18±19）的细胞质中均有明显的survivin指数，其中腺粘膜的survivin指数显著高于腺粘膜（P = 0.001; P = 0.041）。相比之下，毛驴在细胞质中表现出更高的survivin指数，但两种黏膜之间无显著差异（P = 0.506）。结论正常海马胃组织中survivin的表达定位于胃固有层和胃上皮基底层，主要表达于胃细胞的细胞质区。然而，需要进一步的技术来研究这种蛋白在马胃溃疡综合征（EGUS）中的潜在作用，以评估其在这些物种的细胞保护和胃适应中的潜在作用。

{"title":"Cytoplasmic and nuclear Survivin expression in healthy gastric mucosae of equids: A comparative study between horses, donkeys, and mules","authors":"A.L. Medina-Bolívar , J.D. Muñoz-Duque , J.R. Martínez-Aranzales","doi":"10.1016/j.jevs.2025.105678","DOIUrl":"10.1016/j.jevs.2025.105678","url":null,"abstract":"<div><h3>Background</h3><div>Information regarding Survivin protein expression in the gastric mucosa of equids is scarce. This protein has been associated with functions related to modulating apoptosis and promoting mitosis in epithelial cells and is considered part of gastric cytoprotection mechanisms in humans and mice, maintaining mucosal integrity and regulating cellular renewal.</div></div><div><h3>Aims/objectives</h3><div>This study aimed to determine the expression of cytoplasmic and nuclear Survivin in gastric mucosae of equids (horses, donkeys, and mules).</div></div><div><h3>Methods</h3><div>A total of 30 healthy stomachs, 10 from each species, were evaluated using histology and immunohistochemistry techniques. Survivin protein was detected with antibody EP119 and with the polymer method.</div></div><div><h3>Results</h3><div>Immunohistochemistry revealed survivin expression in the gastric mucosa of equids. In horses and mules, the survivin index was evident in the cytoplasm of both glandular (59 ± 55; 55 ± 40) and squamous (15 ± 16; 18 ± 19) mucosa, being significantly higher in the glandular mucosa (P = 0.001; P = 0.041), respectively. In contrast, donkeys showed a higher survivin index in the cytoplasm, but no significant difference was observed between the two types of mucosa (P = 0.506).</div></div><div><h3>Conclusion</h3><div>Survivin expression in the healthy gastric tissue of equids was localized to the lamina propria and the basal layer of the epithelial layer, predominantly within the cytoplasmic compartment of the cells. However, further techniques are needed to study this protein in Equine Gastric Ulcer Syndrome (EGUS) to assess its potential role in cytoprotection and gastro-adaptation in these species.</div></div>","PeriodicalId":15798,"journal":{"name":"Journal of Equine Veterinary Science","volume":"153 ","pages":"Article 105678"},"PeriodicalIF":1.6,"publicationDate":"2025-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144895785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphometric characteristics of quarter horses in barrel racing events: impacts on performance and competitive success 四分之一马在桶装赛中的形态特征：对表现和竞技成功的影响。

IF 1.6 3区农林科学 Q2 VETERINARY SCIENCES

Journal of Equine Veterinary Science

Pub Date : 2025-08-16 DOI: 10.1016/j.jevs.2025.105676

Giovanna Polo Freitag , Luis Gustavo Freitag de Lima , Luiz Ernandes Kozicki , Fernando Andrade Souza , Eriklis Nogueira , Leonir Bueno Ribeiro

Aims

This study investigated the influence of morphometric characteristics on performance in Quarter Horses competing in barrel racing.

Methods

A total of 125 horses were assessed using 28 linear and angular body measurements derived from standardized left-side photographs captured via smartphone and analyzed with open-source software.

Results

Measurements were subjected to exploratory factor analysis, extracting six principal components (forequarters, hindquarters, midsection, posterior angles, scapular traits, age and weight), which together explained 83.0 % of the morphological variance. Forequarter morphology, including withers height and limb lengths, accounted for 37.4 % of the variation in race performance. Hierarchical cluster analysis grouped horses by trial times, revealing that animals with faster times exhibited significantly larger ribcage dimensions, longer scapulae, and greater coxofemoral angles. A multivariate regression model incorporating the principal components predicted individual potential trial times with high accuracy (R² = 0.6848; P = 0.0388), and comparisons with actual times confirmed the model’s validity. Age and body weight were also relevant, with younger, well-muscled horses demonstrating superior performance.

Conclusion

Forequarter conformation, scapular inclination, and indicators of respiratory and muscular capacity were strong predictors of barrel racing success in Quarter Horses, and image-based morphometric analysis was a valuable tool for performance prediction and selection in this study.

目的：研究形态计量学特征对四分之一马桶装赛成绩的影响。方法：通过智能手机拍摄的标准化左侧照片，对125匹马进行28个线性和角度的身体测量，并使用开源软件进行分析。结果：对测量结果进行探索性因子分析，提取出6个主成分（前躯、后躯、中躯、后角、肩胛骨特征、年龄和体重），共解释了83.0%的形态变异。前肢形态，包括肩高和肢长，占比赛成绩变化的37.4%。分层聚类分析根据试验时间对马进行分组，结果显示，试验时间越快的马，其胸腔尺寸明显越大，肩胛骨长度越长，肩关节角也越大。结合主成分的多元回归模型预测个体潜在试验次数准确率较高（R² = 0.6848;P = 0.0388），与实际试验次数比较证实了模型的有效性。年龄和体重也有关系，年轻、肌肉发达的马表现出更好的表现。结论：前肢构象、肩胛骨倾角、呼吸和肌肉容量等指标是四分之一马竞速成功的有力预测指标，基于图像的形态计量学分析是本研究中预测和选择成绩的有价值的工具。

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引用次数: 0

Readers Information 读者信息

IF 1.6 3区农林科学 Q2 VETERINARY SCIENCES

Journal of Equine Veterinary Science

Pub Date : 2025-08-15 DOI: 10.1016/S0737-0806(25)00325-9

引用次数: 0

Butylated hydroxytoluene (BHT) improved semen quality and sperm DNA of frozen-thawed Arabian stallions preserved in modified INRA-82 extender 丁基羟基甲苯（BHT）改善了在改良INRA-82扩展器中保存的冻融阿拉伯种马的精液质量和精子DNA。

IF 1.6 3区农林科学 Q2 VETERINARY SCIENCES

Journal of Equine Veterinary Science

Pub Date : 2025-08-14 DOI: 10.1016/j.jevs.2025.105675

Amal M. Aboelmaaty , Islam El-Sayed El-Seadawy , Mohamed Saeed Kotp , Alaa A. Mohamed , Hazem Ahmed El-Debaky , Diya A. El-Badry , Abeer M. Anwer , Tsvetan Tsvetkov , Teodora Daneva , Mahmoud A. Elgabry

Background

Alpha-tocopherol is one of the non-enzymatic lipophilic antioxidants. Butylated hydroxytoluene (BHT) is a synthetic analog with similar modes of action in protecting the cryopreserved sperms.

Aims/objectives

This study hypothesized that a certain concentration of any antioxidant is suitable for improving the post-thaw semen quality of stallions.

Methods

To determine the optimum BHT concentration, a synthetic antioxidant similar to vitamin E in potency and scavenging oxidative stress power in concentrations of 0.0, 0.25, 0.50, 1.0, 2.0, and 4.0 mM/ml were added to semen extender. The post-thaw sperm progressive motility at 0, 1 h, 2 h, and 3 h, the sperm viability index, the plasma membrane integrity tested by the hypo-osmotic swelling test (HOST), the acrosome integrity, non-fragmented DNA, % of DNA in the comet head, % of DNA in the comet tail, comet tail length, and comet tail moment were compared.

Results

According to our hypothesis, 1.0 mM BHT was the most suitable concentration that preserved the highest (P < 0.0001) post-thaw sperm progressive motility at 0.0, 1 h, 2 h, 3 h., the highest viability index (P < 0.0001), plasma membrane and acrosome integrities (P < 0.0001),non-fragmented DNA (P < 0.001), and % of DNA in the comet head (P < 0.001), the lowest DNA % in the comet tail (P < 0.001) and comet tail moment (P < 0.0001), and the shortest (P < 0.0001) comet tail length.

Conclusions

The concentrations of BHT around 1.0 mM/ml (0.5, 2.0mM/ml) proved better post-thaw semen characteristics, but increasing BHT concentrations from >2.0mM/ml to 4.0 mM/ml started to deteriorate semen quality than non-supplemented control. The optimum BHT concentration for modified INRA-82 is 1.0 mM/ml.

背景：α -生育酚是一种非酶促亲脂抗氧化剂。丁基羟基甲苯（BHT）是一种合成类似物，在保护冷冻保存精子方面具有类似的作用模式。目的：本研究假设一定浓度的抗氧化剂可改善种马解冻后精液质量。方法：在精液膨化剂中分别添加浓度为0.0、0.25、0.50、1.0、2.0和4.0 mM/ml的合成抗氧化剂，以确定BHT的最佳浓度。比较解冻后精子0、1h、2h、3h的进展活力、精子活力指数、低渗透膨胀试验（HOST）检测的质膜完整性、顶体完整性、非片段化DNA、彗头DNA百分比、彗尾DNA百分比、彗尾长度和彗尾瞬间。结论：1.0mM /ml（0.5、2.0mM/ml）前后BHT浓度较好，但当BHT浓度从0 ~ 2.0mM/ml增加到4.0 mM/ml时，精液质量较未添加BHT的对照组开始恶化。改性INRA-82的最佳BHT浓度为1.0 mM/ml。

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引用次数: 0

Photodynamic therapy in the treatment of cutaneous habronemosis in a horse: Case report 光动力疗法治疗马皮肤线虫病一例报告。

IF 1.6 3区农林科学 Q2 VETERINARY SCIENCES

Journal of Equine Veterinary Science

Pub Date : 2025-08-13 DOI: 10.1016/j.jevs.2025.105674

I. Ferreira , J.G. Pinto , E.G.L. Oliveira , M.C.B.S. de Azevedo , L.M.C. Marcolino , J. Ferreira-Strixino

Habronema muscae is a parasitic nematode that causes habronemiasis in horses, leading to chronic skin lesions. The parasite is transmitted via flies, and larvae deposited in wounds result in difficult-to-heal “summer sores”. This case study involved treating a 9-year-old gelding male horse with recurrent habronemiasis using photodynamic therapy (PDT) concurrent with conventional treatments. The horse presented with ulcerated, exudative wounds on its limbs and face, unresponsive to traditional treatments. PDT was applied over six months, using methylene blue (MB) as a photosensitizer, combined with LED irradiation. Additionally, an ivermectin-based ointment and other healing agents were used. The results showed a reduction in lesion size after two months, complete healing of the thoracic wounds by month four, and facial wound healing after six months. In this case, PDT was an effective adjunct treatment for managing habronemiasis.

肌肉Habronema muscae是一种寄生虫，可引起马的Habronema病，导致慢性皮肤损伤。这种寄生虫通过苍蝇传播，幼虫沉积在伤口上导致难以愈合的“夏季疮”。本病例研究涉及使用光动力疗法（PDT）结合常规治疗治疗一匹复发性habronemasis的9岁公马。这匹马的四肢和面部出现溃烂、渗出的伤口，对传统治疗无效。PDT应用超过6个月，使用亚甲基蓝（MB）作为光敏剂，结合LED照射。此外，还使用了伊维菌素软膏和其他愈合剂。结果显示，2个月后病变大小减小，4个月后胸部伤口完全愈合，6个月后面部伤口愈合。在这种情况下，PDT是一种有效的辅助治疗管理血红素病。

引用次数: 0

Emergence of a new alveolar dental element after extraction of a molar tooth in a horse 拔除马的一颗臼齿后出现新的牙槽。

IF 1.6 3区农林科学 Q2 VETERINARY SCIENCES

Journal of Equine Veterinary Science

Pub Date : 2025-08-11 DOI: 10.1016/j.jevs.2025.105673

L.L.V. Oliveira , M.C.A.R. Caldas , L.M.E. Marval , C.A. Marval , J.G.S. Carvalho , J.J.R.T. Matos , B.D. Malacarne , C.F.R. Paz

A 2-year-old mixed-breed mare was examined at the property due to swelling and fistula on the right mandible. Oral examination and radiographs revealed the presence of an apical abscess involving the 410 tooth. The tooth was extracted entirely and confirmed radiographically. One year later, the owner reported a reoccurrence of clinical signs. A new radiographic evaluation revealed a radiopaque area in the alveolar socket of the 410 tooth filled with spongy-looking bone. An extraoral approach to the alveolar socket was performed, and the content removed had the appearance of a dental element.

一匹2岁的混血母马因右下颌骨肿胀和瘘管而被检查。口腔检查及x光片显示410颗牙齿有根尖脓肿。整颗牙齿被拔了出来并做了放射检查。一年后，业主报告临床症状再次出现。新的x线片评估显示410牙的牙槽窝有一个不透射线的区域，里面充满了海绵状的骨头。通过口外入路到达牙槽窝，取出的内容物具有牙体的外观。

引用次数: 0