Journal of Equine Veterinary Science最新文献_第3页

Myo-Inositol Nano-Emulsion for Enhancing Stallion Chilled Semen: Insights from In Vivo and In Silico Analyses of Sperm Quality, Oxidative Stress, Ultrastructure, Mitochondrial Response, and Semen Bacteriology 增强种马冷冻精液的肌醇纳米乳：精子质量、氧化应激、超微结构、线粒体反应和精液细菌学的体内和计算机分析。

IF 1.6 3区农林科学 Q2 VETERINARY SCIENCES

Journal of Equine Veterinary Science

Pub Date : 2026-01-05 DOI: 10.1016/j.jevs.2026.105766

W.A. Khalil , M.I. Sharf , M.K. Derbala , M.A. Alfattah , M.A.E. Hassan , W. Alhujaili , M.A. El-Harairy , S.A. Abdelnour

Background: Oxidative stress is a major contributor to male infertility. Therefore, fortifying assisted reproductive technology with nanotechnology could enhance sperm preservation.

Aims/objectives: This study aimed to examine the impact of myo-inositol nano-emulsion (MINE) supplementation in semen extender on sperm quality, redox balance, semen bacteriology, apoptosis, ultrastructure, and acrosome status of chilled stallion semen.

Methods: Semen samples were collected and preserved with 0 (MINE0), 1 (MINE1), and 2 (MINE2) mg of MINE/mL of extender.

Results: Results revealed that extender fortified with 1 or 2 mg of MINE significantly improved sperm progressive motility, viability, and membrane integrity after 72 hours of cooling (p < 0.05). All MINE levels significantly reduced the sperm abnormalities of chilled stallions’ semen (p < 0.01) and significantly improved the number of intact acrosomes (p < 0.05). Fortified chilled stallion semen with MINE significantly boosted antioxidant defenses (total antioxidant capacity and catalase), while significantly lowering oxidative stress markers (malondialdehyde, nitric oxide, and hydrogen peroxide) (p < 0.05). MINE significantly reduced the populations of total bacteria, coliform, and spore-forming bacteria in the treated groups (p < 0.01). The molecular docking study showed that myo-inositol has a strong binding affinity to key mitochondrial proteins (GPX2, ALDH1A1, UQCRC2, VDAC2, and AKAP3), with calculated binding energies of -4.0, -3.62, -2.94, -2.72, and -2.31 kcal/mol indicating antioxidant defense mechanisms. The inclusion of MINE preserved the sperm ultrastructure, as evidenced by normal acrosome, mitochondria, nucleus, and plasma membrane integrity when observed using SEM.

Conclusion: MINE's potent antioxidants and anti-apoptotic properties effectively mitigate oxidative stress and boost mitochondrial function in stallion sperm cells.

背景：氧化应激是男性不育的主要原因。因此，用纳米技术强化辅助生殖技术可以提高精子的保存能力。目的：本研究旨在研究在精液扩展剂中添加肌醇纳米乳（MINE）对冷藏种马精液的精子质量、氧化还原平衡、精液细菌学、细胞凋亡、超微结构和顶体状态的影响。方法：采集精液标本，用0 （MINE0）、1 （MINE1）、2 (MINE2) mg /mL的扩展剂保存。结果表明，在冷却72小时后，添加1或2 mg矿化矿的扩展剂可显著提高精子的进行性运动性、活力和膜完整性(p)。结论：矿化矿具有有效的抗氧化和抗凋亡特性，可有效减轻种马精子细胞的氧化应激，提高线粒体功能。

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引用次数: 0

Urine sediment characterisation in physically healthy adult jennies (Equus asinus). A cross-sectional study 身体健康的成年珍妮马（马属）尿液沉积物特征。横断面研究。

IF 1.6 3区农林科学 Q2 VETERINARY SCIENCES

Journal of Equine Veterinary Science

Pub Date : 2026-01-03 DOI: 10.1016/j.jevs.2025.105761

I.M. Țăpuc , A.N. Mureșan , M. Mircean , I. Papuc

Urinalysis is a valuable, non-invasive method for evaluating renal function, yet data on donkey urinary parameters remain scarce. This study characterised urinary sediment in fifty-four healthy adult jennies, established urine specific gravity (USG) reference intervals, and proposed a preliminary semi-quantitative sediment grading scale. Urine samples were collected via catheterisation and analysed using refractometry, dipstick testing, and microscopy. The median USG was 1.048 (IQR: 1.040–1.055) with a pH of 7.6 (IQR: 7.2–8.0). Leukocytes and glucose were absent; erythrocytes were found in one sample. Calcium carbonate crystals were present in all samples, followed by calcium oxalate monohydrate (74.07%), mucus filaments (66.67%), and epithelial cells (57.41%). Granular casts were observed in 27.77% and hyaline casts in 7.41% of samples.

Healthy jennies show USG values similar to horses. Urinary sediment had abundant calcium carbonate and notable calcium oxalate crystals. These findings establish baseline sediment profiles and support the future validation of a grading scale for sediment evaluation.

尿液分析是一种有价值的、非侵入性的评估肾功能的方法，然而关于驴尿参数的数据仍然很少。本研究对54名健康成年女性的尿液沉积物进行了表征，建立了尿液比重（USG）参考区间，并提出了初步的半定量沉积物分级量表。尿液样本通过导尿收集，并使用折射仪、试纸测试和显微镜进行分析。中位USG为1.048 (IQR: 1.040-1.055)， pH为7.6 （IQR: 7.2-8.0）。白细胞和葡萄糖缺失；在一个样本中发现了红细胞。所有样品中均存在碳酸钙晶体，其次是一水草酸钙（74.07%）、粘液丝（66.67%）和上皮细胞（57.41%）。27.77%为粒状铸件，7.41%为透明铸件。健康珍妮的USG值与马相似。尿液沉积物中含有丰富的碳酸钙和显著的草酸钙晶体。这些发现建立了基线沉积物剖面，并支持沉积物评价分级尺度的未来验证。

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引用次数: 0

A pilot study to investigate the effect of insemination on secretory cell activity post-ovulation in the isthmus of the equine oviduct 研究人工授精对马输卵管峡部排卵后分泌细胞活性影响的初步研究。

IF 1.6 3区农林科学 Q2 VETERINARY SCIENCES

Journal of Equine Veterinary Science

Pub Date : 2026-01-03 DOI: 10.1016/j.jevs.2026.105765

C.J.P. Jones , J.D. Aplin , S. Wilsher

There is little information on non-ciliated secretory cell numbers in the equine oviduct isthmus over the course of the estrous cycle and the effect, if any, of insemination. Lectin histochemistry was used on a series of 13 oviducts taken at different stages of the estrous cycle, with and without insemination, to monitor glycosylation changes and the distribution of secretory cells characterised by a prominent cytoplasmic accumulation of glycoconjugates. There appeared to be an increase in secretory cells expressing a wide range of glycans in all specimens at estrus. One day after ovulation, observation suggested a marked decrease in binding of most of the lectins except in inseminated specimens in which a subset of specific intracellular sugar structures (containing terminal fucose and/or certain N-glycans) seemed to be preserved at a level similar to that seen during estrus. At diestrus, there was an apparent further reduction in the number of lectin-positive secretory cells. Spermatozoa in the oviduct can produce changes in the oviductal transcriptome in mice, as well as altering the extracellular vesicle cargo released from oviductal epithelial cells. Hence, it is possible that the upregulation and maintenance of fucosylated, N-linked glycans in the inseminated specimens may reflect a continuing need for glycoprotein secretion into oviductal fluid in the horse. These preliminary data suggest that changes in the fluid may play a role in maintaining the sperm reservoir, the events leading to capacitation and possibly early embryo development.

在整个发情周期的过程中，马的输卵管峡部的非纤毛分泌细胞数量和人工授精的影响（如果有的话）的信息很少。采用凝集素组织化学方法，对13条输卵管在不同的发情周期阶段（有和没有授精）进行检测，以监测糖基化变化和分泌细胞的分布，其特征是糖结合物的显著细胞质积累。在发情期，所有标本中表达多种聚糖的分泌细胞似乎都有所增加。排卵后一天，观察显示大多数凝集素的结合明显减少，除了在受精标本中，特定的细胞内糖结构亚群（包含末端聚焦和/或某些n -聚糖）似乎保留在与发情期间相似的水平。在绝育时，凝集素阳性分泌细胞的数量明显进一步减少。输卵管中的精子可以引起小鼠输卵管转录组的变化，并改变输卵管上皮细胞释放的细胞外囊泡货物。因此，受精标本中集中的n链聚糖的上调和维持可能反映了马体持续需要将糖蛋白分泌到输卵管液中。这些初步数据表明，液体的变化可能在维持精子库中起作用，导致获能和可能的早期胚胎发育的事件。

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引用次数: 0

Weekly riding frequency has a greater impact than rider skill level on western riders' identification of stirrup asymmetry 每周骑行频率对西方骑手对马镫不对称识别的影响大于骑手技术水平。

IF 1.6 3区农林科学 Q2 VETERINARY SCIENCES

Journal of Equine Veterinary Science

Pub Date : 2026-01-02 DOI: 10.1016/j.jevs.2025.105758

Emily N Jolley, Ariel H Higgins, Alyssa A Logan

Background

Asymmetry of stirrups and rider experience level has shown to impact rider symmetry and position.

Objectives

This study aims to determine if novice and advanced riders can detect stirrup asymmetry in the same frequency, hypothesizing that advanced riders would detect asymmetry in a greater frequency.

Methods

Ten stock-type horses and 10 (5 advanced, 5 novice) riders, paired together to test random stirrup treatments. Riders were assigned to a horse for 2 rides, one with asymmetric stirrups, the other with symmetric stirrups, but blinded to their treatments. At the end of both rides, they took a survey and answered questions about the ride and symmetry.

Results

Correct stirrup identification was found in 60 % of advanced and 50 % of novice riders (P = 0.10). Advanced riders were found to ride a greater hours per week than novice (P = 0.033).

Conclusion

Weekly riding frequency may impact asymmetry identification, not just experience.

背景：马镫的不对称性和骑手的经验水平影响骑手的对称性和位置。目的：本研究旨在确定新手和高级骑手是否可以在相同的频率下检测到马镫不对称，假设高级骑手会在更高的频率下检测到不对称。方法：10匹家畜型马和10名（5名高级骑手，5名新手）骑手配对进行随机马镫治疗试验。骑手们被分配到一匹马上骑两次，一次骑不对称的马镫，另一次骑对称的马镫，但对他们的治疗不知情。在两次骑行结束后，他们接受了一项调查，并回答了有关骑行和对称性的问题。结果：60%的高级骑手和50%的新手骑手正确识别马镫（P = 0.10）。高级骑手每周骑行的时间比新手多（P = 0.033）。结论：每周骑行频率可能影响不对称识别，而不仅仅是体验。

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引用次数: 0

Readers Information 读者信息

IF 1.6 3区农林科学 Q2 VETERINARY SCIENCES

Journal of Equine Veterinary Science

Pub Date : 2026-01-01 DOI: 10.1016/S0737-0806(26)00007-9

引用次数: 0

Addition of cholesterol linked to cyclodextrin in processing of cryopreserved equine spermatozoa and addition of motility stimulants post-thaw 在冷冻保存马精子的加工过程中添加与环糊精相关的胆固醇和解冻后添加运动刺激剂。

IF 1.6 3区农林科学 Q2 VETERINARY SCIENCES

Journal of Equine Veterinary Science

Pub Date : 2025-12-31 DOI: 10.1016/j.jevs.2025.105760

V.M.T. Vilela , B.M. Zutter , L.L.O. Gomes , D.F. Silva , J.H.W. Diniz , Y.F.R. Sancler-Silva , F.S. Ignácio , F.O. Papa , G.A. Monteiro

Aim

This study investigated the effects of cholesterol-loaded cyclodextrin (CLC) before cryopreservation and the use of FertTalp (FT) after thawing on the structural and functional quality of equine spermatozoa.

Methods

Two ejaculates from twelve stallions were divided into four groups: G1 (0 mg CLC), G2 (1.0 mg), G3 (1.5 mg), and G4 (2.0 mg). Post-thaw semen quality was evaluated through computer-assisted sperm analysis and flow cytometry.

Results

In Experiment I, supplementation with 1.5 mg (G3) significantly improved total motility (64.9 ± 10.1 %) and progressive motility (40.5 ± 8.8 %) compared with the control group (G1) (55.5 ± 15.7 % and 31.9 ± 10.8 %; P < 0.05). No differences were observed for mitochondrial potential or membrane lipid disorder. Cholesterol supplementation did not influence overall longevity during the thermoresistance test, although G3 maintained higher velocity parameters (VSL, VCL, VAP) at several time points compared with G2 (P < 0.05). In Experiment II, FT did not alter sperm kinetics during the first 40 min after thawing; however, at 80 and 120 min, FT-treated samples presented higher proportions of rapid spermatozoa, greater VSL and VAP, and increased linearity (P < 0.05). FT also increased the proportion of viable spermatozoa without intracellular H₂O₂, although the magnitude of this effect was small.

Conclusions

Supplementation with 1.5 mg of CLC before freezing improved initial post-thaw motility without affecting mitochondrial activity or membrane organization. Post-thaw FT enhanced velocity-related parameters and supported sperm functional longevity. The combined approach may represent a practical strategy to optimize the post-thaw performance of stallion semen.

目的：研究高胆固醇环糊精（CLC）冷冻前和解冻后使用FertTalp （FT）对马精子结构和功能质量的影响。方法：将12匹马的2次射精分为G1 （0 mg CLC）、G2 （1.0 mg CLC）、G3 （1.5 mg CLC）和G4 (2.0 mg CLC) 4组。通过计算机辅助精子分析和流式细胞术评估解冻后精液质量。结果：实验一，与对照组（G1组）（55.5±15.7%、31.9±10.8%,P < 0.05）相比，添加1.5 mg （G3）可显著提高总运动性（64.9±10.1%）和进行运动性（40.5±8.8%）。在线粒体电位或膜脂紊乱方面没有观察到差异。在抗热试验期间，尽管G3在几个时间点保持较高的速度参数（VSL、VCL、VAP），但补充胆固醇对总体寿命没有影响（P < 0.05）。在实验二中，在解冻后的前40分钟，FT没有改变精子动力学；然而，在80和120分钟，ft处理的样品呈现出更高的快速精子比例，更大的VSL和VAP，线性增加（P < 0.05）。FT也增加了没有细胞内H₂O₂的活精子的比例，尽管这种影响的幅度很小。结论：冷冻前补充1.5 mg CLC可改善解冻后的初始运动性，而不影响线粒体活性或膜组织。解冻后FT增强了速度相关参数，支持精子功能寿命。该方法为优化种马精液解冻后性能提供了一种实用的策略。

{"title":"Addition of cholesterol linked to cyclodextrin in processing of cryopreserved equine spermatozoa and addition of motility stimulants post-thaw","authors":"V.M.T. Vilela , B.M. Zutter , L.L.O. Gomes , D.F. Silva , J.H.W. Diniz , Y.F.R. Sancler-Silva , F.S. Ignácio , F.O. Papa , G.A. Monteiro","doi":"10.1016/j.jevs.2025.105760","DOIUrl":"10.1016/j.jevs.2025.105760","url":null,"abstract":"<div><h3>Aim</h3><div>This study investigated the effects of cholesterol-loaded cyclodextrin (CLC) before cryopreservation and the use of FertTalp (FT) after thawing on the structural and functional quality of equine spermatozoa.</div></div><div><h3>Methods</h3><div>Two ejaculates from twelve stallions were divided into four groups: G1 (0 mg CLC), G2 (1.0 mg), G3 (1.5 mg), and G4 (2.0 mg). Post-thaw semen quality was evaluated through computer-assisted sperm analysis and flow cytometry.</div></div><div><h3>Results</h3><div>In Experiment I, supplementation with 1.5 mg (G3) significantly improved total motility (64.9 ± 10.1 %) and progressive motility (40.5 ± 8.8 %) compared with the control group (G1) (55.5 ± 15.7 % and 31.9 ± 10.8 %; P < 0.05). No differences were observed for mitochondrial potential or membrane lipid disorder. Cholesterol supplementation did not influence overall longevity during the thermoresistance test, although G3 maintained higher velocity parameters (VSL, VCL, VAP) at several time points compared with G2 (P < 0.05). In Experiment II, FT did not alter sperm kinetics during the first 40 min after thawing; however, at 80 and 120 min, FT-treated samples presented higher proportions of rapid spermatozoa, greater VSL and VAP, and increased linearity (P < 0.05). FT also increased the proportion of viable spermatozoa without intracellular H₂O₂, although the magnitude of this effect was small.</div></div><div><h3>Conclusions</h3><div>Supplementation with 1.5 mg of CLC before freezing improved initial post-thaw motility without affecting mitochondrial activity or membrane organization. Post-thaw FT enhanced velocity-related parameters and supported sperm functional longevity. The combined approach may represent a practical strategy to optimize the post-thaw performance of stallion semen.</div></div>","PeriodicalId":15798,"journal":{"name":"Journal of Equine Veterinary Science","volume":"157 ","pages":"Article 105760"},"PeriodicalIF":1.6,"publicationDate":"2025-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145892514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Femoral nerve neuropathy of an endurance horse as a sequela of iliopsoas muscle myopathy diagnosed by transrectal ultrasonography 经直肠超声诊断为髂腰肌肌病后遗症的一匹耐力马股神经病变

IF 1.6 3区农林科学 Q2 VETERINARY SCIENCES

Journal of Equine Veterinary Science

Pub Date : 2025-12-31 DOI: 10.1016/j.jevs.2025.105757

M. Puccetti , F. Beccati , N. Pilati

Femoral nerve neuropathy causes lameness, a condition clinically characterized by stifle's lack of extension with subsequent diminished lower limb function. This case report describes the ultrasonographic diagnosis of femoral neuropathy secondary to compression caused by myopathy of the iliopsoas muscle of an endurance horse during a competition. Transrectal ultrasonography played a pivotal role in diagnosis of the injury by assessment of the involved structures and monitoring of the healing process throughout the recovery period. The horse achieved complete recovery and successfully returned to competition one year post-injury. Transrectal ultrasonography is recommended to evaluate involvement of the femoral nerve in cases with clinical signs suggesting nerve dysfunction.

股神经病变导致跛行，临床表现为膝关节缺乏伸展，随后下肢功能减弱。本病例报告描述的超声诊断股神经病变继发压迫引起的髂腰肌肌病的一匹耐力马在比赛中。经直肠超声检查通过评估受累结构和监测整个恢复期的愈合过程，在损伤诊断中发挥了关键作用。这匹马在受伤一年后完全康复并成功重返赛场。经直肠超声检查建议评估累及股神经的临床症状提示神经功能障碍。

引用次数: 0

The use of hay nets and slow feeders as feeding methods in horse management: A semi-systematic review 在马管理中使用干草网和慢喂器作为喂养方法：半系统回顾

IF 1.6 3区农林科学 Q2 VETERINARY SCIENCES

Journal of Equine Veterinary Science

Pub Date : 2025-12-30 DOI: 10.1016/j.jevs.2025.105762

Joyce Amaje , Sarah Upton , Umaru Musa Garba , Kelvin Olutimilehin Jolayemi

Background

Hay nets and slow feeders are designed to mimic natural grazing by prolonging forage intake, thereby supporting digestive health and reducing stress-related behaviours. Their design and use, however, can influence effectiveness and may introduce risks.

Aim

This semi-systematic review evaluated the use of hay nets and slow feeders in feeding horses, focusing on their impact on feeding time, behaviour, forage management and health management.

Methods

A semi-systematic search across PubMed, ScienceDirect, Google Scholar, and Scopus identified 26 full-text studies published between 2009 and 2025.

Results

Results showed that horse feeding time was extended by 40% depending on mesh size, multi-layered nets, and forage type, with ponies generally experiencing greater extended feeding time than horses. Slow feeders consistently reduced forage wastage from 57% to as low as 6%, with economic analyses suggesting potential recovery of the purchasing cost for the feeding device within a year. Behavioural benefits included reductions in cribbing, and weaving, although overly restrictive designs sometimes provoked frustration, pawing, or net flinging. Ground-based slow feeders encouraged a natural head-down posture, whereas elevated nets increased neck tension and pulling force, raising concerns about musculoskeletal strain. Dental impacts appeared minimal, but long-term outcomes remain unclear. For weight and metabolic health, some studies reported net-fed horses losing 20-23 kg compared with weight gain in floor-fed controls, while others found no changes.

Conclusion

Overall, hay nets and slow feeders provide clear welfare and management benefits, but design and application must balance efficiency with equine wellbeing. Further research should refine their use across breeds, management systems, and individual horse need.

干草网和慢食器的设计是为了模仿自然放牧，延长采食量，从而支持消化系统健康，减少与压力相关的行为。然而，它们的设计和使用会影响有效性，并可能带来风险。目的本半系统综述评估了干草网和慢速喂食器在喂养马时的使用，重点关注它们对喂养时间、行为、饲料管理和健康管理的影响。方法对PubMed、ScienceDirect、b谷歌Scholar和Scopus进行半系统检索，确定了2009年至2025年间发表的26篇全文研究。结果根据网眼尺寸、多层网眼和饲料类型的不同，马的进食时间延长了40%，其中小马的进食时间延长幅度大于马。慢速喂食者持续将饲料浪费从57%降低到6%，经济分析表明，在一年内，喂食设备的购买成本可能会收回。行为上的好处包括减少了抓取和编织，尽管过度限制的设计有时会引起挫折、抓或扔网。地面上的慢食者鼓励自然的头朝下的姿势，而升高的网增加了颈部的张力和拉力，引起了对肌肉骨骼紧张的担忧。对牙齿的影响很小，但长期结果尚不清楚。在体重和代谢健康方面，一些研究报告称，与地板喂养的对照组相比，净喂养的马体重增加了20-23公斤，而其他研究则没有发现任何变化。结论总体而言，干草网和慢食器提供了明显的福利和管理效益，但设计和应用必须平衡效率和马的健康。进一步的研究应该改进它们在不同品种、管理系统和个体马需求中的使用。

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引用次数: 0

Serum cortisol concentrations in newborn foals are increased by transfusion with anti-rhodococcal hyperimmune plasma and inversely associated with immunoglobulin activity 新生马驹的血清皮质醇浓度因输血抗红球菌高免疫血浆而升高，并与免疫球蛋白活性呈负相关。

IF 1.6 3区农林科学 Q2 VETERINARY SCIENCES

Journal of Equine Veterinary Science

Pub Date : 2025-12-30 DOI: 10.1016/j.jevs.2025.105759

Kira C. Sayre , Patricia Flores-Ahlschwede , Alexandra B. Wethington , Susanne K. Kahn , Jocelyne M. Bray , Devynn M. Volding , Scott Ahlschwede , Angela I. Bordin , Noah D. Cohen , Thomas H. Welsh Jr.

Background

Transfusion of Rhodococcus equi hyperimmune plasma (REHIP) to neonatal foals is done to reduce the incidence of rhodococcal pneumonia.

Objectives

To determine effects in newborn foals of REHIP transfusion on serum cortisol concentrations; the association of serum cortisol concentrations with serum globulin concentration and anti-rhodococcal immunoglobulin activity; and cumulative incidence of rhodococcal pneumonia.

Methods

Serum samples were collected from 356 newborn foals from 13 New York farms immediately before and after transfusion of 1 L or 2 L of REHIP. Serum samples were assayed for concentrations of cortisol, total globulins and immunoglobulin activities against virulence associated protein A (VapA). Data were analyzed using linear mixed-effects regression, chi-squared testing, and Spearman rank correlation coefficient.

Results

REHIP transfusion increased (P < 0.05) cortisol concentration from a mean of 21.9 ng/mL to 44.1 ng/mL. Post-transfusion serum cortisol concentration was less (P < 0.05) for foals transfused with 1 L REHIP than with 2 L REHIP. Pre-transfusion serum cortisol was negatively associated (P < 0.05) with total globulins (r = −0.19) and anti-rhodococcal activities of immunoglobulin G₁ (IgG_1; r = −0.22) and immunoglobulin G_4/7 (IgG_4/7; r = −0.16). Pre- and post-transfusion cortisol concentrations were not associated with pneumonia development.

Conclusions

Cortisol concentrations are significantly increased by REHIP transfusion, and serum cortisol concentrations are inversely associated with serum immunoglobulin concentrations in foals. However, serum cortisol concentration was not associated with increased risk of rhodococcal pneumonia in this population of foals.

背景：对新生马驹输注马红球菌高免疫血浆（REHIP）是为了降低红球菌肺炎的发生率。目的：探讨REHIP对新生马驹血清皮质醇浓度的影响；血清皮质醇浓度与血清球蛋白浓度和抗红球菌免疫球蛋白活性的关系以及红球菌肺炎的累积发病率。方法：分别采集纽约13个马场356匹新生马驹在输血1 L或2 L REHIP前后的血清样本。检测血清样本皮质醇浓度、总球蛋白和抗毒力相关蛋白A （VapA）的免疫球蛋白活性。数据分析采用线性混合效应回归、卡方检验和Spearman秩相关系数。结果：REHIP输血使皮质醇浓度由21.9 ng/mL升高至44.1 ng/mL （P < 0.05）。注射1 L REHIP的马驹输血后血清皮质醇浓度低于注射2 L REHIP的马驹（P < 0.05）。输血前血清皮质醇与总球蛋白（r = -0.19）、免疫球蛋白G1 （IgG1, r = -0.22）和免疫球蛋白G4/7 （IgG4/7, r = -0.16）抗红球菌活性呈负相关（P < 0.05）。输血前后皮质醇浓度与肺炎发展无关。结论：输血后马驹皮质醇浓度显著升高，血清皮质醇浓度与血清免疫球蛋白浓度呈负相关。然而，在这群马驹中，血清皮质醇浓度与红球菌肺炎风险增加无关。

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引用次数: 0

Upwards or onwards? Assessment of objective gait quality parameters in three European horse breeds at walk and trot 向上还是向前？三种欧洲马在步行和小跑时的客观步态质量参数评价。

IF 1.6 3区农林科学 Q2 VETERINARY SCIENCES

Journal of Equine Veterinary Science

Pub Date : 2025-12-30 DOI: 10.1016/j.jevs.2025.105764

AI Gmel , EH Haraldsdóttir , TV Rosa , LP Lamas , M Neuditschko , MA Weishaupt

Background

Europe has many horse breeds differing in conformation, gaits and genetics due to diverging breeding goals. Research studies comparing objective gait parameters between different horse breeds are limited and date back a decade.

Aim and methods

In this study, objective gait parameters were measured in three European breeds, namely Lusitanos (LUS; n = 52), Franches-Montagnes (FM; n = 311) and Swiss Warmblood (WB; n = 40) using an inertial measurement system (sensors at poll, withers, pelvis and all four canon bones) at walk and trot.

Results

At walk and trot, LUS moved significantly slower than WB and FM (mean -0.65 m/s at walk, mean -1.35 m/s at trot), while FM had the highest stride frequencies and WB made longer strides at lower frequency. Furthermore, FM and WB also had significantly larger hind limb protraction angles than LUS. Warmblood horses had significantly larger front limb protraction angles at walk than FM and LUS, but not at trot. At trot, FM had significantly longer suspension durations but smaller vertical range of motion measured by the withers’ sensor, suggesting a more horizontal, efficient way of moving, compared to the more aerial and dressage movements of LUS and WB. Each breed has evolved gait patterns to the demands of their most prominent equestrian sport (e.g. collected vertical movements for LUS, horizontal pulling of carriages for FM, vertical elasticity and ground coverage for show jumping and dressage for WB).

Conclusion

The assessment of objective gait parameters revealed clear breed-specific differences, thereby advancing the exploration of the genetic architecture of movement patterns in horses.

背景：欧洲有许多不同的马品种，由于不同的育种目标，在构象、步态和遗传上有所不同。比较不同马品种之间客观步态参数的研究是有限的，并且可以追溯到十年前。目的与方法：在本研究中，采用惯性测量系统（在轮头、马颈、骨盆和所有四个正骨处设有传感器）测量了三个欧洲品种Lusitanos （LUS; n = 52）、Franches-Montagnes （FM; n = 311）和Swiss Warmblood （WB; n = 40）在步行和小跑时的客观步态参数。结果：在步行和小跑时，LUS的步频明显慢于WB和FM（步行时平均-0.65 m/s，小跑时平均-1.35 m/s），而FM步频最高，WB步频较低。此外，FM和WB的后肢伸展角也显著大于LUS。温血马在步行时前肢伸角显著大于FM和LUS，而在小跑时则无显著差异。在小跑时，FM的悬挂时间明显更长，但由马肩马传感器测量的垂直运动范围更小，这表明与LUS和WB的空中和盛装舞步运动相比，FM的运动方式更水平、更有效。每个品种都进化了步态模式，以满足他们最突出的马术运动的要求（例如，LUS的垂直运动，FM的水平拉车，WB的垂直弹性和地面覆盖）。结论：客观步态参数的评估揭示了明显的品种特异性差异，从而推进了马运动模式遗传结构的探索。

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引用次数: 0