Journal of Food Biochemistry最新文献

Sinomenine is extracted from the root of Chinese herb QingTeng (Sinomenium acutum (Thunb.) Rehd. Et wils.), which is extensively utilized by traditional medical practitioners for its anti-inflammatory and immunoregulatory properties in China for decades. The aim of this study was to assess the effects and potential mechanisms of sinomenine in alleviating dextran sodium sulfate (DSS)–induced chronic colitis and memory disorder in mice. A mouse chronic colitis model was established by 2.5% DSS, and sinomenine treatment was oral administration. It showed that sinomenine inhibited weight loss, colon shortening, proinflammatory cytokine production, and intestinal barrier injury in the colon. Sinomenine improved inflammatory bowel diseases (IBD) and accompanying memory disorders with downregulated expressions of proinflammatory cytokines and neuronal apoptosis; upregulated expressions of brain-derived neurotrophic factor (BDNF) and ZO-1 in the hippocampus. Additionally, our findings indicated that sinomenine altered the composition of various gut microbiota, leading to an increase in Bacteroidetes and Firmicutes at the phylum level, and Rikenella at the genus level. Moreover, sinomenine ameliorated the SCFA content in DSS-induced mice both in serum and feces. The findings demonstrated that sinomenine ameliorated DSS-induced chronic colitis and accompanying memory disorders.

RETRACTION: U. Saleem, Z. Gull, A. Saleem, et al. “Appraisal of anti-Parkinson activity of rhinacanthin-C in haloperidol-induced parkinsonism in mice: A mechanistic approach,” Journal of Food Biochemistry, 45, (2021): e13677, https://doi.org/10.1111/jfbc.13677.

The above article, published online on 12 March 2021 in Wiley Online Library (https://wileyonlinelibrary.com), has been retracted by agreement between the journal Editor-in-Chief, Prisca-Maryla Henheik, and John Wiley & Sons Ltd. The retraction has been agreed due to concerns with Figure 3, first identified on PubPeer [1].

Ammara Saleem, Muhammad Furqan Akhtar, Pharkphoom Panichayupakaranant and Fareeha Anwar disagree with the retraction. The remaining authors did not respond.

RETRACTION: T. Mishra, K. Nagarajan, P. K. Dixit, and V. Kumar, “Neuroprotective potential of ferulic acid against cyclophosphamide-induced neuroinflammation and behavioral changes,” Journal of Food Biochemistry 46, no. 12 (2022), https://doi.org/10.1111/jfbc.14436.

The above article, published online on 27 September 2022 in Wiley Online Library (https://wileyonlinelibrary.com), has been retracted following the agreement of the journal’s Chief Editor, Prisca-Maryla Henheik; and John Wiley & Sons Ltd.

The retraction has been agreed following an investigation by the publisher, which identified unexpected overlap within figures. Specifically, the hippocampal tissue in Figure 6a contains overlapping features with the tissue shown in Figure 6e, in which it is attributed to a different experimental condition.

The authors provided a response to these concerns; however, this did not satisfy the editorial board. As such, the results of the article are considered unreliable, and it is therefore retracted.

The authors disagree with this retraction.

Cisplatin is a commonly used chemotherapy medication that damages the DNA of cancer cells; however, the nonselective toxic damage that it causes to both cancerous and normal cells leads to adverse side effects, such as inflammation of the intestinal mucosa. Intestinal inflammation reduces zonula occluden-1 (ZO-1) expression and impairs the integrity of tight junctions, resulting in intestinal barrier dysfunction. Lutein, a carotenoid with anti-inflammatory effects, has been proven beneficial in preventing eye diseases; however, whether lutein can ameliorate cisplatin-induced damage to intestinal epithelial cells remains unclear. This study thus investigated the protective effects of lutein against cisplatin-induced damage in normal IEC-6 rat small intestine epithelial cells. Cisplatin treatment reduced ZO-1 mRNA and protein levels without significantly affecting other junction proteins and aquaporins and suppressed wound healing capacity. Furthermore, cisplatin-induced intestinal barrier damage was linked to the activation of the p38 and ERK pathways, which promoted nuclear factor κ-light-chain-enhancer of activated B cells (NF-κB) nuclear translocation and increased its transcriptional activity. Importantly, pretreatment with lutein significantly reversed the suppressive effects of cisplatin on ZO-1 expression and improved wound healing by inhibiting the activation of p38 and ERK, as well as the transcriptional activity of NF-κB. These findings indicate that lutein protects against cisplatin-induced intestinal barrier damage. These insights support the potential use of lutein as a nutritional supplement to alleviate the gastrointestinal side effects of cisplatin chemotherapy.

This study explored the enhancement of anthocyanin and polyphenol extraction from Clitoria ternatea L. by comparing microwave-assisted extraction with enzymatic support (MEAE) to microwave-assisted extraction (MAE). Optimal conditions for extraction were determined via response surface methodology and included an enzyme concentration of 1.26% (w/w), pH 3.5, temperature of 52°C, and extraction time of 20 min. Under these conditions, the extract yielded a total anthocyanin content (TAC) of 3.47 ± 0.09 mg/g dry matter and a total polyphenol content (TPC) of 68.64 ± 3.27 mg GAE/g dry matter, confirming the model’s predictive reliability. Response surface analysis indicated that both temperature and extraction time significantly affected TAC, as evidenced by steep and curved interaction surfaces. The addition of MEAE significantly enhanced efficiency compared to MEA. Scanning electron microscopy revealed extensive disruption of plant cell walls, supporting the synergistic effect of microwave and enzymatic treatment on enhancing mass transfer. Moreover, UPLC and LC–HRMS analyses identified ten polyphenolic compounds and seven anthocyanins in the extract. These findings highlight MEAE as a practical and synergistic approach for maximizing the recovery of anthocyanins and polyphenols from Clitoria ternatea flowers.

This study was designed to recover bioactive compounds, including artemisinin, phenolics, tannins, and amino acids (AAs), from the aerial parts of Artemisia annua L. (sweet wormwood) and Artemisia abrotanum L. (southern wormwood) using ultrasound-assisted extraction (UAE). Optimal extraction was achieved using 60% ethanol (EtOH) and 60 min of UAE, yielding the highest total phenolic content (TPC) of 43.55 mg GAE/g DW for A. annua and 33.11 mg GAE/g DW for A. abrotanum, along with total tannin content of 34.63 and 25.16 mg CE/g DW, respectively. Antioxidant activity, assessed via DPPH^• and FRAP assays, was significantly higher under these conditions. DPPH^• and FRAP values reached 11.29 and 15.57 mg TE/g DW for A. annua and 8.17 and 11.91 mg TE/g DW for A. abrotanum, respectively. Artemisinin was quantified exclusively in A. annua, with a maximum of 9.51 mg/100 g DW after 40 min of UAE with 96% EtOH, and 21 mg/100 g DW following scale-up and concentration. Free AA profiling revealed proline as the dominant AA in both species, with concentrations of 350.56 mg/100 g DW in A. annua and 427.80 mg/100 g DW in A. abrotanum. A. annua showed higher contents of essential and branched-chain AAs, at 163.75 and 88.43 mg/100 g DW, compared to 127.38 and 63.86 mg/100 g DW in A. abrotanum, respectively. Antimicrobial assays demonstrated moderate inhibition, with minimum inhibitory and bactericidal concentrations ranging from 12.5 to 25.0 mg/mL for A. annua and 12.5–50.0 mg/mL for A. abrotanum, depending on the microorganism. UAE allowed to effectively recover bioactive compounds and AAs from A. annua and A. abrotanum, with A. annua exhibiting superior TPC, AA, and antimicrobial profiles. These findings support the potential of Artemisia species as sources of antioxidant and antimicrobial agents.

This study evaluated the bioaccessibility and antioxidant capacity of the ethanolic extracts of Tagetes patula (ETP) and Zingiber officinale (EZO) during simulated in vitro gastrointestinal digestion. The phenolic content and antioxidant capacity were analyzed using the DPPH and FRAP methods at the oral, gastric, and intestinal stages. Simulated digestion led to a reduction in phenolic and flavonoid levels in both extracts. The T. patula extract retained 64.05 ± 4.63 mg GAE/g DE of phenolic compounds and 49.18 ± 7.97 mg RE/g DE of flavonoids in the UF. In contrast, Z. officinale exhibited higher susceptibility, with phenolic content decreasing from 130.13 ± 4.69 to 16.96 ± 0.14 mg GAE/g DE and flavonoid content from 64.97 ± 5.75 to 0.32 ± 0.09 mg RE/g DE. Nevertheless, during the final stage of digestion, an increase in DPPH radical scavenging capacity was observed compared to previous stages, reaching 23.29 ± 0.42 mg TE/g DE. Notably, high-performance liquid chromatography (HPLC) analysis revealed that T. patula maintained its free phenolic compounds throughout digestion, while Z. officinale showed enhanced release of these compounds.

This study aimed to compare the enhancement of immune responses by hydroponically grown ginseng (HGG) extract, which has increased ginsenoside content, with that by red ginseng extract (RGE). This research utilizes hydroponic cultivation instead of conventional soil-based methods to enhance the ginsenoside content in ginseng seedlings, thereby increasing their immunomodulatory effects. This innovative cultivation methodology is expected to create a new standard for the future use of ginseng as a functional food ingredient. To this end, we evaluated the effect of hydroponically grown ginseng seedling extract (HGSE) and hydroponically grown ginseng plantlet extract (HGPE) on immune control in RAW 264.7 macrophages and mouse bone marrow–derived macrophages (BMDMs). Treatment with both HGSE and HGPE resulted in phagocytic activity superior to that of RGE in the presence of E. coli and stimulated tumor necrosis factor (TNF)-α production in a concentration-dependent manner under 12.5–50 μg/mL treatment conditions. In addition, treatment of BMDMs with HGSE and HGPE resulted in unrivaled enhancement of both TNF-α and interleukin-6 production, and both HGSE and HGPE have been shown to upregulate mitogen-activated protein kinase (MAPK) signaling pathways. In the final analysis, the efficacy of the HGPE extract was evaluated in the immunosuppressed BALB/c mouse model. Oral administration of the extract alleviated the significant reduction in spleen weight induced by cyclophosphamide. Additionally, cytokine array results demonstrated regulation of adiponectin, ICAM-1, and IGFBP-1 regulation. According to the results of the study, HGG seedlings can act as a suitable eco-friendly next-generation option for red ginseng as a functional food ingredient.

Garcinone C, a xanthone derived from Garcinia mangostana L, possesses antioxidant and anticancer effects. However, its role in gastric cancer remains unexplored. This study aimed to investigate the effects of garcinone C on gastric cancer cell proliferation and its underlying mechanism. We found that garcinone C suppressed gastric cancer cell growth by inducing G0/G1 arrest and apoptosis in a dose-dependent manner. Furthermore, garcinone C downregulated G0/G1 phase markers cyclin D1 and p21, as well as apoptosis markers Bax, Bcl-2, cleaved-PARP, and c-caspase 3. Following the previous evidence demonstrated that aberrant Hedgehog (Hh) signaling is implicated in gastric cancer development, we confirmed that inhibiting Gli1/2 reduced the growth of AGS and MKN74 cells. Furthermore, garcinone C exerted similar effects to Gant61, inhibiting Hh signaling by reducing Gli1/2 levels and blocking their nuclear translocation. These findings suggest that garcinone C inhibits gastric cancer proliferation via the Hh signaling, indicating its potential as a therapeutic agent for treating gastric cancer.