首页 > 最新文献

Journal of Food Biochemistry最新文献

英文 中文
Structural Characterization and Transcript-Metabolite Analysis of Macrophage Immunomodulation Induced by Luffa aegyptiaca Polysaccharide 丝瓜多糖诱导巨噬细胞免疫调节的结构表征及转录代谢物分析
IF 4.2 2区 农林科学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2025-09-04 DOI: 10.1155/jfbc/3740603
Qingyan Deng, Ming Cheng, Yinghao Wang, Li Li, Yingnian Lu

Luffa aegyptiaca exhibits broad medicinal and therapeutic potential, with its polysaccharide component identified as a key contributor to its biological activities. However, the specific molecular structures and immunomodulatory mechanisms of these polysaccharides remain largely unclear, which hinders their further development for medicinal development. In this study, a novel polysaccharide, named LAP-C1, was extracted and purified from L. aegyptiaca using a DEAE-52 cellulose column. Its molecular characteristics and immunomodulation mechanism were systematically investigated using various analytical techniques, combined with integrated transcriptomic and metabolomic analyses. LAP-C1 contained 56.17% total carbohydrates and 38.33% uronic acids. Monosaccharide composition analysis revealed that LAP-C1 primarily consists of fucose (3.74%), arabinose (11.71%), rhamnose (16.16%), galactose (37.60%), glucose (4.76%), galacturonic acid (23.44%), and glucuronic acid (2.57%). The immunomodulatory assay demonstrated that LAP-C1 enhanced the phagocytic ability of RAW264.7 macrophages, the nitric oxide (NO) production, and cytokine secretion (IL-6, IL-1β, and TNF-α). Transcriptomic and metabolomic analyses revealed that LAP-C1 exerts its immunomodulatory effects primarily by activating the alpha-linolenic acid and arachidonic acid metabolism pathways. This study provides critical insights into the molecular structure and immunostimulatory mechanisms of Luffa-derived polysaccharides, thereby laying a foundation for their potential application in immunomodulatory therapies and biomedical development.

埃及丝瓜具有广泛的药用和治疗潜力,其多糖成分被认为是其生物活性的关键因素。然而,这些多糖的具体分子结构和免疫调节机制仍不清楚,这阻碍了其进一步的药物开发。本研究利用DEAE-52纤维素柱从埃及乳杆菌中提取并纯化了一种新的多糖LAP-C1。利用多种分析技术,结合转录组学和代谢组学综合分析,系统地研究了其分子特性和免疫调节机制。LAP-C1的总碳水化合物含量为56.17%,醛酸含量为38.33%。单糖组成分析表明,LAP-C1主要由焦糖(3.74%)、阿拉伯糖(11.71%)、鼠李糖(16.16%)、半乳糖(37.60%)、葡萄糖(4.76%)、半乳糖醛酸(23.44%)和葡萄糖醛酸(2.57%)组成。免疫调节实验表明,LAP-C1增强RAW264.7巨噬细胞的吞噬能力、一氧化氮(NO)的产生和细胞因子(IL-6、IL-1β和TNF-α)的分泌。转录组学和代谢组学分析显示,LAP-C1主要通过激活α -亚麻酸和花生四烯酸代谢途径发挥其免疫调节作用。该研究为丝瓜多糖的分子结构和免疫刺激机制提供了重要的见解,从而为其在免疫调节治疗和生物医学开发中的潜在应用奠定了基础。
{"title":"Structural Characterization and Transcript-Metabolite Analysis of Macrophage Immunomodulation Induced by Luffa aegyptiaca Polysaccharide","authors":"Qingyan Deng,&nbsp;Ming Cheng,&nbsp;Yinghao Wang,&nbsp;Li Li,&nbsp;Yingnian Lu","doi":"10.1155/jfbc/3740603","DOIUrl":"https://doi.org/10.1155/jfbc/3740603","url":null,"abstract":"<p><i>Luffa aegyptiaca</i> exhibits broad medicinal and therapeutic potential, with its polysaccharide component identified as a key contributor to its biological activities. However, the specific molecular structures and immunomodulatory mechanisms of these polysaccharides remain largely unclear, which hinders their further development for medicinal development. In this study, a novel polysaccharide, named LAP-C1, was extracted and purified from <i>L. aegyptiaca</i> using a DEAE-52 cellulose column. Its molecular characteristics and immunomodulation mechanism were systematically investigated using various analytical techniques, combined with integrated transcriptomic and metabolomic analyses. LAP-C1 contained 56.17% total carbohydrates and 38.33% uronic acids. Monosaccharide composition analysis revealed that LAP-C1 primarily consists of fucose (3.74%), arabinose (11.71%), rhamnose (16.16%), galactose (37.60%), glucose (4.76%), galacturonic acid (23.44%), and glucuronic acid (2.57%). The immunomodulatory assay demonstrated that LAP-C1 enhanced the phagocytic ability of RAW264.7 macrophages, the nitric oxide (NO) production, and cytokine secretion (IL-6, IL-1β, and TNF-α). Transcriptomic and metabolomic analyses revealed that LAP-C1 exerts its immunomodulatory effects primarily by activating the alpha-linolenic acid and arachidonic acid metabolism pathways. This study provides critical insights into the molecular structure and immunostimulatory mechanisms of <i>Luffa</i>-derived polysaccharides, thereby laying a foundation for their potential application in immunomodulatory therapies and biomedical development.</p>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2025 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/3740603","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144934871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Research Progress on the Relationship Between Variations in Breast Milk Composition and Food Allergies in Children 母乳成分变化与儿童食物过敏关系的研究进展
IF 4.2 2区 农林科学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2025-08-28 DOI: 10.1155/jfbc/3741893
Jun Fang, Xuan Zhang

The escalating global prevalence of infant food allergies (FA) imposes societal burdens and health risks, highlighting the importance of understanding breast milk (BM) composition in FA prevention and early intervention. As the latest systematic review summarizing molecular mechanisms and clinical evidence, it comprehensively addressed the relationship between FA pathogenesis and BM composition, including key metabolites (short-chain fatty acids [SCFAs], human milk oligosaccharides [HMOs], and free amino acids [FAAs]), fat-soluble vitamins, microbiota composition, and food allergens. Five main mechanisms were described as follows: (1) HMOs shape immune polarization through TLR4 signaling and Th cell balance regulation; (2) lipids mediate immune responses via SCFA-G protein-coupled receptor (GPCR) pathways and polyunsaturated fatty acids (PUFA)–dependent membrane effects; (3) FAAs sustain gut barrier function and modulate IgE responses through metabolic regulation; (4) fat-soluble vitamins maintain immune homeostasis through nuclear receptor signaling and mucosal maintenance; and (5) microbiota-HMO crosstalk generates tolerance-promoting metabolites. Interestingly, the amount of clinical evidence also indicated that certain BM components can be involved in regulating FA. These insights offered clinically actionable targets for precision nutrition and redefined BM as a dynamic immune-modulating ecosystem with stratified intervention potential.

婴儿食物过敏(FA)的全球流行率不断上升,给社会带来了负担和健康风险,这凸显了了解母乳(BM)成分在预防和早期干预婴儿食物过敏中的重要性。作为总结分子机制和临床证据的最新系统综述,它全面阐述了FA发病与BM组成的关系,包括关键代谢物(短链脂肪酸[SCFAs]、人乳低聚糖[HMOs]和游离氨基酸[FAAs])、脂溶性维生素、微生物群组成和食物过敏原。主要机制如下:(1)HMOs通过TLR4信号传导和细胞平衡调节形成免疫极化;(2)脂质通过SCFA-G蛋白偶联受体(GPCR)途径和多不饱和脂肪酸(PUFA)依赖的膜效应介导免疫应答;(3) FAAs通过代谢调节维持肠道屏障功能并调节IgE反应;(4)脂溶性维生素通过核受体信号和粘膜维持维持免疫稳态;(5)微生物- hmo串扰产生促进耐受性的代谢物。有趣的是,大量的临床证据也表明,某些脑基质成分可能参与调节FA。这些见解为精确营养提供了临床可操作的目标,并将BM重新定义为具有分层干预潜力的动态免疫调节生态系统。
{"title":"Research Progress on the Relationship Between Variations in Breast Milk Composition and Food Allergies in Children","authors":"Jun Fang,&nbsp;Xuan Zhang","doi":"10.1155/jfbc/3741893","DOIUrl":"https://doi.org/10.1155/jfbc/3741893","url":null,"abstract":"<p>The escalating global prevalence of infant food allergies (FA) imposes societal burdens and health risks, highlighting the importance of understanding breast milk (BM) composition in FA prevention and early intervention. As the latest systematic review summarizing molecular mechanisms and clinical evidence, it comprehensively addressed the relationship between FA pathogenesis and BM composition, including key metabolites (short-chain fatty acids [SCFAs], human milk oligosaccharides [HMOs], and free amino acids [FAAs]), fat-soluble vitamins, microbiota composition, and food allergens. Five main mechanisms were described as follows: (1) HMOs shape immune polarization through TLR4 signaling and Th cell balance regulation; (2) lipids mediate immune responses via SCFA-G protein-coupled receptor (GPCR) pathways and polyunsaturated fatty acids (PUFA)–dependent membrane effects; (3) FAAs sustain gut barrier function and modulate IgE responses through metabolic regulation; (4) fat-soluble vitamins maintain immune homeostasis through nuclear receptor signaling and mucosal maintenance; and (5) microbiota-HMO crosstalk generates tolerance-promoting metabolites. Interestingly, the amount of clinical evidence also indicated that certain BM components can be involved in regulating FA. These insights offered clinically actionable targets for precision nutrition and redefined BM as a dynamic immune-modulating ecosystem with stratified intervention potential.</p>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2025 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/3741893","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144910256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Exploring the Flavor Substances in “Laocaifu” Based on UPLC-MS Combined With GC-MS 基于超高效液相色谱-质谱联用的“老菜菜”风味物质研究
IF 4.2 2区 农林科学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2025-08-27 DOI: 10.1155/jfbc/6668787
Wei-Feng Cai, Zhi-Fei Xiong, Yi Liu, Rui-Xuan Hu, Yuan-Yuan An, Jia-Yi Li, Chun-Yan Shen, Yan-Kui Yi

Background: Laocaifu (LCF), a traditional Chinese fermented radish product originating from the Fujian and Chaoshan regions, is widely consumed for its distinctive flavor and texture. However, the specific flavor substances and quality markers associated with different aging periods still remained poorly characterized.

Purpose: This study aimed to characterize the key flavor substances and quality markers in LCF across different aging durations.

Methods: The chemical profiles of LCF samples aged 2, 6, 10, 20, and 30 years were comparatively characterized using ultraviolet (UV), high-performance liquid chromatography (HPLC), ultra-performance liquid chromatography–mass spectrometry (UPLC–MS), and gas chromatography–mass spectrometry (GC–MS) methods. Simultaneously, sensory evaluation was performed to analyze the changes of color, aroma, flavor, and texture characteristics of LCF at different years; to verify the differences of flavor substances; and to provide a sensory basis for subsequent chemical analysis.

Results: Sensory evaluation revealed progressive darkening of color, intensification of sourness, and softening of texture with extended aging of LCF. HPLC assay identified potential signature components eluting at 14–16 min, while UPLC–MS assay detected acid derivatives (such as azelaic acid and hexadecanedioic acid) and alcohols as major flavor contributors. Notably, hexadecanedioic acid and azelaic acid showed a strong positive correlation with prolonged aging, establishing their potential as a reliable aging biomarker. Age-dependent variations in ester and alcohol contents were observed, especially in LCF at 6 and 10 years, suggesting their influence on sensory characteristics. Furthermore, GC–MS assay showed that compositional diversity increased with the aging time of LCF.

Conclusion: This study successfully established UPLC–MS as an effective tool for LCF quality assessment by correlating chemical profiles with sensory attributes. The identification of hexadecanedioic acid and azelaic acid as key aging biomarkers, with content directly proportional to fermentation duration, provided a scientific basis for quality control and market differentiation of LCF products. These findings address critical gaps in the commercial characterization of this traditional fermented food.

背景:老菜夫是一种传统的发酵萝卜产品,原产于福建和潮汕地区,以其独特的风味和口感而被广泛消费。然而,与不同陈酿期相关的特定风味物质和品质指标的表征仍然很差。目的:研究不同陈酿时间下LCF中的关键风味物质和品质指标。方法:采用紫外(UV)、高效液相色谱(HPLC)、超高效液相色谱-质谱(UPLC-MS)、气相色谱-质谱(GC-MS)等方法对2、6、10、20、30年LCF样品的化学特征进行比较分析。同时进行感官评价,分析不同年份LCF的色、香、味和质地特征的变化;验证风味物质的差异;并为后续的化学分析提供一个感官基础。结果:感官评价显示,随着时间的延长,LCF的颜色逐渐变暗,酸味增强,质地软化。高效液相色谱法确定了14-16分钟洗脱的潜在特征成分,而超高效液相色谱-质谱法检测到酸衍生物(如壬二酸和十六烯二酸)和醇是主要的风味成分。值得注意的是,十六烷二酸和壬二酸与延长衰老呈强正相关,这表明它们有潜力成为可靠的衰老生物标志物。观察到酯和醇含量的年龄依赖性变化,特别是在6岁和10岁的LCF中,这表明它们对感官特征的影响。GC-MS分析表明,随着LCF老化时间的延长,其成分多样性逐渐增加。结论:UPLC-MS通过将化学特征与感官属性相关联,成功地建立了LCF质量评价的有效工具。十六烯二酸和壬二酸作为关键的老化生物标志物,其含量与发酵时间成正比,为LCF产品的质量控制和市场差异化提供了科学依据。这些发现解决了这种传统发酵食品在商业特性方面的关键空白。
{"title":"Exploring the Flavor Substances in “Laocaifu” Based on UPLC-MS Combined With GC-MS","authors":"Wei-Feng Cai,&nbsp;Zhi-Fei Xiong,&nbsp;Yi Liu,&nbsp;Rui-Xuan Hu,&nbsp;Yuan-Yuan An,&nbsp;Jia-Yi Li,&nbsp;Chun-Yan Shen,&nbsp;Yan-Kui Yi","doi":"10.1155/jfbc/6668787","DOIUrl":"https://doi.org/10.1155/jfbc/6668787","url":null,"abstract":"<p><b>Background:</b> Laocaifu (LCF), a traditional Chinese fermented radish product originating from the Fujian and Chaoshan regions, is widely consumed for its distinctive flavor and texture. However, the specific flavor substances and quality markers associated with different aging periods still remained poorly characterized.</p><p><b>Purpose:</b> This study aimed to characterize the key flavor substances and quality markers in LCF across different aging durations.</p><p><b>Methods:</b> The chemical profiles of LCF samples aged 2, 6, 10, 20, and 30 years were comparatively characterized using ultraviolet (UV), high-performance liquid chromatography (HPLC), ultra-performance liquid chromatography–mass spectrometry (UPLC–MS), and gas chromatography–mass spectrometry (GC–MS) methods. Simultaneously, sensory evaluation was performed to analyze the changes of color, aroma, flavor, and texture characteristics of LCF at different years; to verify the differences of flavor substances; and to provide a sensory basis for subsequent chemical analysis.</p><p><b>Results:</b> Sensory evaluation revealed progressive darkening of color, intensification of sourness, and softening of texture with extended aging of LCF. HPLC assay identified potential signature components eluting at 14–16 min, while UPLC–MS assay detected acid derivatives (such as azelaic acid and hexadecanedioic acid) and alcohols as major flavor contributors. Notably, hexadecanedioic acid and azelaic acid showed a strong positive correlation with prolonged aging, establishing their potential as a reliable aging biomarker. Age-dependent variations in ester and alcohol contents were observed, especially in LCF at 6 and 10 years, suggesting their influence on sensory characteristics. Furthermore, GC–MS assay showed that compositional diversity increased with the aging time of LCF.</p><p><b>Conclusion:</b> This study successfully established UPLC–MS as an effective tool for LCF quality assessment by correlating chemical profiles with sensory attributes. The identification of hexadecanedioic acid and azelaic acid as key aging biomarkers, with content directly proportional to fermentation duration, provided a scientific basis for quality control and market differentiation of LCF products. These findings address critical gaps in the commercial characterization of this traditional fermented food.</p>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2025 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/6668787","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144905488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Optimization of Hemp Seed (Cannabis sativa L.) Protein Extraction and Glycation Conditions by Response Surface Methodology: Physicochemical, Functional, and Thermal Characteristics 大麻种子(Cannabis sativa L.)的优化响应面法的蛋白质提取和糖基化条件:理化、功能和热特性
IF 4.2 2区 农林科学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2025-08-26 DOI: 10.1155/jfbc/5493335
Elvan Yüzer, Naciye Kutlu, Mustafa Onur Yüzer

In this study, first, optimization using the response surface method was implemented to obtain the highest yield of protein isolate from defatted hemp seed under different extraction conditions. Optimum extraction conditions were determined with 0% salt concentration, 12 pH value, and 49°C temperature. The protein isolate produced under optimum conditions was subjected to the glycation process, and the glycation conditions were also optimized. The optimum conditions for glycation were determined as a protein isolate: sugar ratio of 1.6:1, temperature of 60°C, and time of 90 min, providing the most suitable glycation degrees and browning indices. There was no statistical difference between the glycated protein isolate obtained under these conditions and the nonglycated protein isolate in terms of emulsifying activity index and emulsion stability index. For total phenolic–flavonoid compounds and antioxidant activity, the sugar complex caused a decrease in these values. In addition, there was a high positive correlation between bioactive compounds. SEM micrographs showed relationships between hemp seed protein isolate and sugar, and these interactions led to a spongy three-dimensional microstructure. When the FTIR results were analyzed, the glycated isolate had stronger absorption at 3300–3200 cm−1 compared to the nonglycated isolate. In conclusion, this study reports important findings in terms of functional and characteristic properties of how monosaccharides affect glycation.

本研究首先利用响应面法对不同提取条件下脱脂大麻籽分离蛋白提取率进行优化。最佳提取条件为:盐浓度为0%,pH值为12,温度为49℃。在最佳条件下得到的分离蛋白进行糖基化,并对糖基化条件进行了优化。确定了分离蛋白糖基化的最佳条件:糖比为1.6:1,温度为60℃,时间为90 min,可获得最合适的糖基化度和褐变指标。在此条件下得到的糖化分离蛋白与非糖化分离蛋白的乳化活性指数和乳化稳定性指数无统计学差异。对于总酚类黄酮化合物和抗氧化活性,糖复合物导致这些值下降。此外,生物活性化合物之间存在高度正相关。扫描电镜显微镜显示大麻籽分离蛋白和糖之间的关系,这些相互作用导致海绵状的三维微观结构。当分析FTIR结果时,与非糖化分离物相比,糖化分离物在3300-3200 cm−1处具有更强的吸收。总之,本研究报告了在单糖影响糖基化的功能和特性方面的重要发现。
{"title":"Optimization of Hemp Seed (Cannabis sativa L.) Protein Extraction and Glycation Conditions by Response Surface Methodology: Physicochemical, Functional, and Thermal Characteristics","authors":"Elvan Yüzer,&nbsp;Naciye Kutlu,&nbsp;Mustafa Onur Yüzer","doi":"10.1155/jfbc/5493335","DOIUrl":"https://doi.org/10.1155/jfbc/5493335","url":null,"abstract":"<p>In this study, first, optimization using the response surface method was implemented to obtain the highest yield of protein isolate from defatted hemp seed under different extraction conditions. Optimum extraction conditions were determined with 0% salt concentration, 12 pH value, and 49°C temperature. The protein isolate produced under optimum conditions was subjected to the glycation process, and the glycation conditions were also optimized. The optimum conditions for glycation were determined as a protein isolate: sugar ratio of 1.6:1, temperature of 60°C, and time of 90 min, providing the most suitable glycation degrees and browning indices. There was no statistical difference between the glycated protein isolate obtained under these conditions and the nonglycated protein isolate in terms of emulsifying activity index and emulsion stability index. For total phenolic–flavonoid compounds and antioxidant activity, the sugar complex caused a decrease in these values. In addition, there was a high positive correlation between bioactive compounds. SEM micrographs showed relationships between hemp seed protein isolate and sugar, and these interactions led to a spongy three-dimensional microstructure. When the FTIR results were analyzed, the glycated isolate had stronger absorption at 3300–3200 cm<sup>−1</sup> compared to the nonglycated isolate. In conclusion, this study reports important findings in terms of functional and characteristic properties of how monosaccharides affect glycation.</p>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2025 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/5493335","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144905482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of Packaging Materials and Storage Durations on the Phytochemical and Antioxidant Profiles of Ethiopian Ginger (Zingiber officinale) Genotypes 包装材料和贮存时间对埃塞俄比亚姜基因型植物化学和抗氧化特性的影响
IF 4.2 2区 农林科学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2025-08-21 DOI: 10.1155/jfbc/8237731
Temesgen Laelago Ersedo, Tilahun A. Teka, Sirawdink Fikreyesus Forsido, Engida Dessalegn

Ginger (Zingiber officinale) is a valuable medicinal and culinary plant renowned for its rich content of bioactive compounds, including phytochemicals and antioxidants, which help neutralize free radicals and promote overall health. The stability of these compounds in dried ginger powder depends significantly on packaging materials and storage conditions. This study evaluated the influence of two packaging types—glass jars and high-density polyethylene (HDPE)—and storage durations of 3, 6, and 9 months on the phytochemical and antioxidant profiles of four Ethiopian ginger genotypes: Boziab, Volvo, Farmers Variety, and Candidate-19. The goal was to determine the most effective packaging and storage combination for preserving the nutritional quality of ginger. Total phenolic content (TPC), total flavonoid content (TFC), ferric reducing antioxidant power (FRAP), and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity were measured. Results indicated a significant reduction in both phytochemical and antioxidant activity with increased storage time, particularly beyond 6 months. Although the differences between glass jars and HDPE packaging were generally not statistically significant (p < 0.05) during the 3- and 6-month periods, glass jars demonstrated a slightly better preservation effect. Among the genotypes, Boziab stored in glass jars for 3 months recorded the highest levels of TPC, TFC, FRAP, and DPPH activity. These findings suggest that ginger should be stored at room temperature for no longer than 6 months to retain its health-promoting compounds. Further studies are recommended to explore extended storage periods, alternative packaging technologies, and strategies to enhance the retention of bioactive constituents.

生姜(Zingiber officinale)是一种有价值的药用和烹饪植物,以其丰富的生物活性化合物而闻名,包括植物化学物质和抗氧化剂,有助于中和自由基,促进整体健康。这些化合物在干姜粉中的稳定性很大程度上取决于包装材料和储存条件。本研究评估了两种包装类型——玻璃罐和高密度聚乙烯(HDPE)——以及3个月、6个月和9个月的储存时间对四种埃塞俄比亚生姜基因型(Boziab、Volvo、Farmers Variety和候选19)的植物化学和抗氧化特性的影响。目的是确定最有效的包装和储存组合,以保持生姜的营养品质。测定了总酚含量(TPC)、总黄酮含量(TFC)、铁还原抗氧化能力(FRAP)和1,1-二苯基-2-苦酰肼(DPPH)自由基清除能力。结果表明,随着储存时间的增加,特别是超过6个月,植物化学和抗氧化活性显著降低。尽管在3个月和6个月期间,玻璃罐和HDPE包装之间的差异通常没有统计学意义(p < 0.05),但玻璃罐的保存效果略好。在基因型中,Boziab在玻璃罐中储存3个月的TPC、TFC、FRAP和DPPH活性最高。这些发现表明,生姜应在室温下存放不超过6个月,以保留其促进健康的化合物。进一步的研究建议探索延长储存期限,替代包装技术和策略,以提高生物活性成分的保留。
{"title":"Effect of Packaging Materials and Storage Durations on the Phytochemical and Antioxidant Profiles of Ethiopian Ginger (Zingiber officinale) Genotypes","authors":"Temesgen Laelago Ersedo,&nbsp;Tilahun A. Teka,&nbsp;Sirawdink Fikreyesus Forsido,&nbsp;Engida Dessalegn","doi":"10.1155/jfbc/8237731","DOIUrl":"https://doi.org/10.1155/jfbc/8237731","url":null,"abstract":"<p>Ginger (<i>Zingiber officinale</i>) is a valuable medicinal and culinary plant renowned for its rich content of bioactive compounds, including phytochemicals and antioxidants, which help neutralize free radicals and promote overall health. The stability of these compounds in dried ginger powder depends significantly on packaging materials and storage conditions. This study evaluated the influence of two packaging types—glass jars and high-density polyethylene (HDPE)—and storage durations of 3, 6, and 9 months on the phytochemical and antioxidant profiles of four Ethiopian ginger genotypes: Boziab, Volvo, Farmers Variety, and Candidate-19. The goal was to determine the most effective packaging and storage combination for preserving the nutritional quality of ginger. Total phenolic content (TPC), total flavonoid content (TFC), ferric reducing antioxidant power (FRAP), and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity were measured. Results indicated a significant reduction in both phytochemical and antioxidant activity with increased storage time, particularly beyond 6 months. Although the differences between glass jars and HDPE packaging were generally not statistically significant (<i>p</i> &lt; 0.05) during the 3- and 6-month periods, glass jars demonstrated a slightly better preservation effect. Among the genotypes, Boziab stored in glass jars for 3 months recorded the highest levels of TPC, TFC, FRAP, and DPPH activity. These findings suggest that ginger should be stored at room temperature for no longer than 6 months to retain its health-promoting compounds. Further studies are recommended to explore extended storage periods, alternative packaging technologies, and strategies to enhance the retention of bioactive constituents.</p>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2025 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/8237731","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144881281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Chemical Characterization of Rheum tanguticum Petiole Juice via Membrane Filtration: A Comparative Analysis Using UPLC–QTOF–MS–MS and Network Pharmacology 膜过滤大黄叶柄汁的化学性质:UPLC-QTOF-MS-MS与网络药理学的对比分析
IF 4.2 2区 农林科学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2025-08-21 DOI: 10.1155/jfbc/8616106
Chen Chen, Zengrong Ye, Jianan Li, Na Liu, Bo Wang, Guoying Zhou

Rheum tanguticum​ petiole juice, widely consumed in Tibet as both a vegetable and an herb, exhibits varying chemical compositions depending on the production methods. This study aims to identify the bioactive components in raw juice using ultra-performance liquid chromatography–quadrupole time-of-flight tandem mass spectrometry (UPLC–QTOF–MS–MS) and analyze their antidiabetic properties through network pharmacology. In addition, the effects of the three types of membrane clarification were evaluated. A total of 36 compounds were identified, and a components–targets–pathways–disease network was constructed for diabetes treatment. Among the membranes tested, the 0.2 μm microfiltration membrane exhibited the highest permeate flux and retained significant amounts of total flavonoids, phenolics, and proanthocyanidins, making it the optimal choice for juice clarification. These findings lay the groundwork for the industrial processing and utilization of R. tanguticum petiole juice in China.

本研究旨在利用超高效液相色谱-四极杆飞行时间串联质谱(UPLC-QTOF-MS-MS)鉴定原料汁中的生物活性成分,并通过网络药理学分析其抗糖尿病作用。此外,还对三种类型的膜澄清效果进行了评价。共鉴定出36个化合物,构建了治疗糖尿病的组分-靶点-通路-疾病网络。其中,0.2 μm微滤膜的渗透通量最高,并保留了大量的总黄酮、酚类物质和原花青素,是果汁澄清的最佳选择。这些研究结果为唐菖蒲叶柄汁的工业加工利用奠定了基础。
{"title":"Chemical Characterization of Rheum tanguticum Petiole Juice via Membrane Filtration: A Comparative Analysis Using UPLC–QTOF–MS–MS and Network Pharmacology","authors":"Chen Chen,&nbsp;Zengrong Ye,&nbsp;Jianan Li,&nbsp;Na Liu,&nbsp;Bo Wang,&nbsp;Guoying Zhou","doi":"10.1155/jfbc/8616106","DOIUrl":"https://doi.org/10.1155/jfbc/8616106","url":null,"abstract":"<p><i>Rheum tanguticum</i>​ petiole juice, widely consumed in Tibet as both a vegetable and an herb, exhibits varying chemical compositions depending on the production methods. This study aims to identify the bioactive components in raw juice using ultra-performance liquid chromatography–quadrupole time-of-flight tandem mass spectrometry (UPLC–QTOF–MS–MS) and analyze their antidiabetic properties through network pharmacology. In addition, the effects of the three types of membrane clarification were evaluated. A total of 36 compounds were identified, and a components–targets–pathways–disease network was constructed for diabetes treatment. Among the membranes tested, the 0.2 μm microfiltration membrane exhibited the highest permeate flux and retained significant amounts of total flavonoids, phenolics, and proanthocyanidins, making it the optimal choice for juice clarification. These findings lay the groundwork for the industrial processing and utilization of <i>R</i>. <i>tanguticum</i> petiole juice in China.</p>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2025 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/8616106","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144881282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The Food Contaminants Chloropropanols and Chloropropanol Esters: A Review 食品污染物氯丙醇和氯丙醇酯综述
IF 4.2 2区 农林科学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2025-08-20 DOI: 10.1155/jfbc/4846670
Jinghui Fan, Jingnan Zhang, Xin Gao, Yang Zhou

Chloropropanols and chloropropanol esters are major food pollutants. Free chloropropanols are found in foods such as soy sauce, infant formula, edible vegetable oils, bread and margarine, whereas chloropropanol esters are found primarily in processed oils and fats. Risk assessments and toxicological studies indicate that these compounds pose significant risks to human health. Their formation during the heat processing of food is inevitable, making them a critical food safety concern. Comprehensive research on their formation mechanisms, detection methods, exposure levels and toxicity profiles is essential to address this issue. This review summarizes the current knowledge of the occurrence, toxicology, detection and elimination of chloropropanols and chloropropanol esters. It provides a scientific basis for further understanding these pollutants, suggests directions for future research and identifies key challenges requiring resolution.

氯丙醇和氯丙醇酯是主要的食品污染物。游离氯丙醇存在于酱油、婴儿配方奶粉、食用植物油、面包和人造黄油等食品中,而氯丙醇酯主要存在于加工过的油和脂肪中。风险评估和毒理学研究表明,这些化合物对人类健康构成重大风险。它们在食品热加工过程中的形成是不可避免的,这使它们成为一个重要的食品安全问题。全面研究其形成机制、检测方法、暴露水平和毒性特征对解决这一问题至关重要。本文综述了氯丙醇和氯丙醇酯的发生、毒理学、检测和消除等方面的最新研究进展。它为进一步了解这些污染物提供了科学基础,为未来的研究提出了方向,并确定了需要解决的关键挑战。
{"title":"The Food Contaminants Chloropropanols and Chloropropanol Esters: A Review","authors":"Jinghui Fan,&nbsp;Jingnan Zhang,&nbsp;Xin Gao,&nbsp;Yang Zhou","doi":"10.1155/jfbc/4846670","DOIUrl":"https://doi.org/10.1155/jfbc/4846670","url":null,"abstract":"<p>Chloropropanols and chloropropanol esters are major food pollutants. Free chloropropanols are found in foods such as soy sauce, infant formula, edible vegetable oils, bread and margarine, whereas chloropropanol esters are found primarily in processed oils and fats. Risk assessments and toxicological studies indicate that these compounds pose significant risks to human health. Their formation during the heat processing of food is inevitable, making them a critical food safety concern. Comprehensive research on their formation mechanisms, detection methods, exposure levels and toxicity profiles is essential to address this issue. This review summarizes the current knowledge of the occurrence, toxicology, detection and elimination of chloropropanols and chloropropanol esters. It provides a scientific basis for further understanding these pollutants, suggests directions for future research and identifies key challenges requiring resolution.</p>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2025 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/4846670","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144869891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Association Between Serum Vitamin C and Depression in Adults: A Cross-Sectional Study of NHANES From 2017 to 2018 成人血清维生素C与抑郁症之间的关系:2017 - 2018年NHANES的横断面研究
IF 4.2 2区 农林科学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2025-08-13 DOI: 10.1155/jfbc/7442661
Dehua Zhao, Xiaoqing Long, Jisheng Wang

Background: Previous studies have examined the relationship between vitamin C and depression, but the findings have been inconclusive. The aim of this cross-sectional study was to investigate the association between serum vitamin C and depression in adults.

Methods: Data from the 2017-2018 National Health and Nutrition Examination Survey (NHANES) cycle were utilized. Weighted multivariable logistic regressions were applied to assess the association between serum vitamin C and depression. Weighted restricted cubic spline (RCS) regression was conducted to explore the dose–response relationship between serum vitamin C and depression. In addition, we performed stratified and sensitivity analyses to evaluate the stability of the results.

Results: A total of 4517 participants were included in this study. Among of them, 410 (9.08%) participants suffered depression. In fully adjusted models, serum vitamin C, as a continuous variable, was negatively associated with depression (OR = 0.69, 95% CI: 0.54–0.88, p = 0.003). Similar results were observed when serum vitamin C was analyzed as a categorical variable. The ORs (95% CI) for Q2, Q3, and Q4, with Q1 as the reference, were 1.00 (0.76–1.33), 0.73 (0.54–0.99), and 0.59 (0.42–0.81), respectively. The RCS analysis found a linear negative relationship between serum vitamin C and depression (p for nonlinearity = 0.579). Additionally, the inverse associations between serum vitamin C and depression remained robust in stratified and sensitivity analyses.

Conclusion: Our findings suggested that there was a linear negative relationship between serum vitamin C and depression, indicating that maintaining sufficient serum vitamin C is recommended due to its potential benefits for protecting against depression.

背景:以前的研究已经调查了维生素C和抑郁症之间的关系,但研究结果尚无定论。本横断面研究的目的是调查成人血清维生素C与抑郁症之间的关系。方法:使用2017-2018年全国健康与营养检查调查(NHANES)周期的数据。应用加权多变量logistic回归来评估血清维生素C与抑郁症之间的关系。采用加权限制三次样条(RCS)回归分析血清维生素C与抑郁症的剂量-反应关系。此外,我们还进行了分层分析和敏感性分析,以评价结果的稳定性。结果:本研究共纳入4517名受试者。其中410人(9.08%)患有抑郁症。在完全调整模型中,血清维生素C作为一个连续变量,与抑郁症呈负相关(OR = 0.69, 95% CI: 0.54-0.88, p = 0.003)。当血清维生素C作为分类变量进行分析时,观察到类似的结果。Q2、Q3和Q4的or (95% CI)以Q1为参照,分别为1.00(0.76-1.33)、0.73(0.54-0.99)和0.59(0.42-0.81)。RCS分析发现血清维生素C与抑郁症呈线性负相关(非线性p = 0.579)。此外,在分层和敏感性分析中,血清维生素C与抑郁症之间的负相关仍然很明显。结论:我们的研究结果表明血清维生素C与抑郁症之间存在线性负相关关系,这表明维持足够的血清维生素C对预防抑郁症有潜在的好处,因此建议保持充足的血清维生素C。
{"title":"Association Between Serum Vitamin C and Depression in Adults: A Cross-Sectional Study of NHANES From 2017 to 2018","authors":"Dehua Zhao,&nbsp;Xiaoqing Long,&nbsp;Jisheng Wang","doi":"10.1155/jfbc/7442661","DOIUrl":"https://doi.org/10.1155/jfbc/7442661","url":null,"abstract":"<p><b>Background:</b> Previous studies have examined the relationship between vitamin C and depression, but the findings have been inconclusive. The aim of this cross-sectional study was to investigate the association between serum vitamin C and depression in adults.</p><p><b>Methods:</b> Data from the 2017-2018 National Health and Nutrition Examination Survey (NHANES) cycle were utilized. Weighted multivariable logistic regressions were applied to assess the association between serum vitamin C and depression. Weighted restricted cubic spline (RCS) regression was conducted to explore the dose–response relationship between serum vitamin C and depression. In addition, we performed stratified and sensitivity analyses to evaluate the stability of the results.</p><p><b>Results:</b> A total of 4517 participants were included in this study. Among of them, 410 (9.08%) participants suffered depression. In fully adjusted models, serum vitamin C, as a continuous variable, was negatively associated with depression (OR = 0.69, 95% CI: 0.54–0.88, <i>p</i> = 0.003). Similar results were observed when serum vitamin C was analyzed as a categorical variable. The ORs (95% CI) for Q2, Q3, and Q4, with Q1 as the reference, were 1.00 (0.76–1.33), 0.73 (0.54–0.99), and 0.59 (0.42–0.81), respectively. The RCS analysis found a linear negative relationship between serum vitamin C and depression (<i>p</i> for nonlinearity = 0.579). Additionally, the inverse associations between serum vitamin C and depression remained robust in stratified and sensitivity analyses.</p><p><b>Conclusion:</b> Our findings suggested that there was a linear negative relationship between serum vitamin C and depression, indicating that maintaining sufficient serum vitamin C is recommended due to its potential benefits for protecting against depression.</p>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2025 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/7442661","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144833249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The Combination of Casticin and Vemurafenib Enhances Apoptotic Cell Death Through the Mitochondria- and Caspases-Mediated Pathways in Human Melanoma A375.S2 Cells In Vitro Casticin和Vemurafenib联合使用通过线粒体和caspase介导的途径增强人黑色素瘤A375中凋亡细胞死亡S2细胞的体外培养
IF 4.2 2区 农林科学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2025-08-13 DOI: 10.1155/jfbc/8831345
Shu-Fen Peng, Hao-Yun Tung, Wen-Wen Huang, Yu-Cheng Chou, Jin-Cherng Lien, Fu-Shin Chueh, Jaw-Chyun Chen, Jing-Gung Chung, Yi-Shih Ma, Chiung-Ju Chen

Vemurafenib (PLX4032) targets the most common BRAF mutation, V600E, in human skin melanoma. Casticin, a polymethoxylated flavonoid compound, is extracted from Viticis fructus (a traditional Chinese medicine) and presents many pharmacological activities, in particular, anticancer activities. However, no report shows that both combinations induce cytotoxic effects in human cancer cells. This study is the first report to illustrate the effects of PLX4032, casticin, or casticin combined with PLX4032 on inducing apoptotic cell death in human skin melanoma A375.S2 cells. Results showed that the treatment of PLX4032 combined with casticin caused cytotoxic activities via suppressing cell viability and inducing DNA condensation and then resulted in cell death in A375.S2 cells. The two-drug combination enhanced the amounts of reactive oxygen species (ROS) and Ca2+ release; however, it diminished the level of mitochondrial membrane potential (ΔΨm). Moreover, the levels of caspase-3, caspase-8, caspase-9, and mitochondria-associated proteins (cytochrome c, AIF, and Endo G) were higher in casticin combined with PLX4032 treatment than that in PLX4032 or casticin treatment only by western blotting. The levels of Fas, FasL, FADD, GADD153, and caspase-4 were elevated in the combined treatment. Confocal laser microscopy also confirms that cytochrome c, AIF, and Endo G had higher expression in the combined drug treatments than in single-drug treatment. The casticin combined with PLX4032 treatment decreased cell viability by triggering apoptotic cell death via both mitochondria- and caspase-dependent pathways in human skin melanoma A375.S2 cells.

Vemurafenib (PLX4032)靶向人类皮肤黑色素瘤中最常见的BRAF突变V600E。castticin是一种多甲氧基类黄酮化合物,从中药葡萄中提取,具有多种药理活性,特别是抗癌活性。然而,没有报告表明这两种组合在人类癌细胞中诱导细胞毒性作用。本研究首次报道了PLX4032、蓖麻素或蓖麻素联合PLX4032诱导人皮肤黑色素瘤A375中凋亡细胞死亡的作用。S2细胞。结果表明,PLX4032与蓖麻素联合处理通过抑制细胞活力和诱导DNA凝聚引起细胞毒活性,最终导致A375细胞死亡。S2细胞。两药联合可提高活性氧(ROS)和Ca2+的释放量;然而,它降低了线粒体膜电位水平(ΔΨm)。此外,caspase-3、caspase-8、caspase-9和线粒体相关蛋白(细胞色素c、AIF和Endo G)的水平在caspase联合PLX4032处理中高于PLX4032或caspase单独用western blotting处理。联合治疗组Fas、FasL、FADD、GADD153、caspase-4水平升高。激光共聚焦显微镜也证实了细胞色素c、AIF和Endo G在联合药物治疗中的表达高于单药治疗。casticin联合PLX4032治疗通过线粒体依赖性和caspase依赖性途径在人皮肤黑色素瘤A375中触发凋亡细胞死亡,从而降低细胞活力。S2细胞。
{"title":"The Combination of Casticin and Vemurafenib Enhances Apoptotic Cell Death Through the Mitochondria- and Caspases-Mediated Pathways in Human Melanoma A375.S2 Cells In Vitro","authors":"Shu-Fen Peng,&nbsp;Hao-Yun Tung,&nbsp;Wen-Wen Huang,&nbsp;Yu-Cheng Chou,&nbsp;Jin-Cherng Lien,&nbsp;Fu-Shin Chueh,&nbsp;Jaw-Chyun Chen,&nbsp;Jing-Gung Chung,&nbsp;Yi-Shih Ma,&nbsp;Chiung-Ju Chen","doi":"10.1155/jfbc/8831345","DOIUrl":"https://doi.org/10.1155/jfbc/8831345","url":null,"abstract":"<p>Vemurafenib (PLX4032) targets the most common BRAF mutation, V600E, in human skin melanoma. Casticin, a polymethoxylated flavonoid compound, is extracted from Viticis fructus (a traditional Chinese medicine) and presents many pharmacological activities, in particular, anticancer activities. However, no report shows that both combinations induce cytotoxic effects in human cancer cells. This study is the first report to illustrate the effects of PLX4032, casticin, or casticin combined with PLX4032 on inducing apoptotic cell death in human skin melanoma A375.S2 cells. Results showed that the treatment of PLX4032 combined with casticin caused cytotoxic activities via suppressing cell viability and inducing DNA condensation and then resulted in cell death in A375.S2 cells. The two-drug combination enhanced the amounts of reactive oxygen species (ROS) and Ca<sup>2+</sup> release; however, it diminished the level of mitochondrial membrane potential (ΔΨm). Moreover, the levels of caspase-3, caspase-8, caspase-9, and mitochondria-associated proteins (cytochrome c, AIF, and Endo G) were higher in casticin combined with PLX4032 treatment than that in PLX4032 or casticin treatment only by western blotting. The levels of Fas, FasL, FADD, GADD153, and caspase-4 were elevated in the combined treatment. Confocal laser microscopy also confirms that cytochrome c, AIF, and Endo G had higher expression in the combined drug treatments than in single-drug treatment. The casticin combined with PLX4032 treatment decreased cell viability by triggering apoptotic cell death via both mitochondria- and caspase-dependent pathways in human skin melanoma A375.S2 cells.</p>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2025 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/8831345","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144833186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Lycium ruthenicum Murray Anthocyanin Regulates the Molecular Mechanism of Hypoxia-Induced Calcium Homeostasis in H9c2 Cells Through the circAgtpbp1/rno-miR-30b-5p/Rgs8 Axis 枸杞花青素通过circAgtpbp1/rno-miR-30b-5p/Rgs8轴调控缺氧诱导H9c2细胞钙稳态的分子机制
IF 4.2 2区 农林科学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2025-08-10 DOI: 10.1155/jfbc/9916493
Jinming Li, Hua Wu, Wensheng Wang, Tong Shen, Jiayi Liu, Zixin Chen, Shuo Wang

Maintaining intracellular calcium (Ca2+) homeostasis is crucial for normal cardiomyocyte function and growth. Anthocyanins, known for their strong antioxidant properties, can neutralize free radicals and mitigate hypoxia-induced damage to cardiomyocytes. Circular RNAs (circRNAs), acting as competing endogenous RNAs (ceRNAs), regulate cellular processes under hypoxic conditions by interacting with microRNAs to influence cell differentiation, proliferation, apoptosis, and energy metabolism. However, the role of circRNAs in intracellular Ca2+ homeostasis remains unclear. In this study, we explored the impact of the circAgtpbp1/rno-miR-30b-5p/Rgs8 axis on the regulation of Ca2+ homeostasis in hypoxia-induced H9c2 cells, based on our previous findings related to anthocyanin-mediated hypoxia protection. We utilized various molecular tools, including circAgtpbp1 and Rgs8 overexpression vectors, si-RNA fragments, and rno-miR-30b-5p mimics and inhibitors, to modulate the expression of circAgtpbp1, Rgs8 and rno-miR-30b-5p. Our results indicate that inhibition of rno-miR-30b-5p, along with overexpression of circAgtpbp1 and Rgs8, disrupts the regulatory effects of anthocyanins on hypoxia-induced H9c2 cells, while promoting these effects conversely. Moreover, circAgtpbp1 and Rgs8 synergistically modulate the influence of anthocyanins on these cells, with rno-miR-30b-5p counteracting the effects of Rgs8 and circAgtpbp1. These findings suggest that anthocyanins regulate Ca2+ homeostasis in hypoxia-induced H9c2 cells through the circAgtpbp1/Rgs8/rno-miR-30b-5p axis and provide insights into the molecular mechanisms underlying anthocyanin-mediated hypoxia protection, thus establishing a foundation for further research on cardiac protection in hypoxic conditions.

维持细胞内钙(Ca2+)稳态对正常的心肌细胞功能和生长至关重要。花青素,以其强大的抗氧化特性而闻名,可以中和自由基,减轻缺氧引起的心肌细胞损伤。环状rna (circRNAs)作为竞争内源性rna (ceRNAs),通过与microRNAs相互作用,影响细胞分化、增殖、凋亡和能量代谢,在缺氧条件下调节细胞过程。然而,环状rna在细胞内Ca2+稳态中的作用尚不清楚。在本研究中,我们基于之前花青素介导的缺氧保护的相关发现,探讨了circAgtpbp1/rno-miR-30b-5p/Rgs8轴对缺氧诱导的H9c2细胞中Ca2+稳态调节的影响。我们利用各种分子工具,包括circAgtpbp1和Rgs8过表达载体、si-RNA片段和rno-miR-30b-5p模拟物和抑制剂,来调节circAgtpbp1、Rgs8和rno-miR-30b-5p的表达。我们的研究结果表明,抑制rno-miR-30b-5p,以及circAgtpbp1和Rgs8的过表达,会破坏花青素对缺氧诱导的H9c2细胞的调节作用,同时反过来促进这些作用。此外,circAgtpbp1和Rgs8协同调节花青素对这些细胞的影响,而rno-miR-30b-5p抵消了Rgs8和circAgtpbp1的作用。这些发现表明,花青素通过circAgtpbp1/Rgs8/rno-miR-30b-5p轴调控缺氧诱导的H9c2细胞Ca2+稳态,揭示了花青素介导的缺氧保护的分子机制,为进一步研究缺氧条件下心脏保护奠定了基础。
{"title":"Lycium ruthenicum Murray Anthocyanin Regulates the Molecular Mechanism of Hypoxia-Induced Calcium Homeostasis in H9c2 Cells Through the circAgtpbp1/rno-miR-30b-5p/Rgs8 Axis","authors":"Jinming Li,&nbsp;Hua Wu,&nbsp;Wensheng Wang,&nbsp;Tong Shen,&nbsp;Jiayi Liu,&nbsp;Zixin Chen,&nbsp;Shuo Wang","doi":"10.1155/jfbc/9916493","DOIUrl":"https://doi.org/10.1155/jfbc/9916493","url":null,"abstract":"<div>\u0000 <p>Maintaining intracellular calcium (Ca<sup>2+</sup>) homeostasis is crucial for normal cardiomyocyte function and growth. Anthocyanins, known for their strong antioxidant properties, can neutralize free radicals and mitigate hypoxia-induced damage to cardiomyocytes. Circular RNAs (circRNAs), acting as competing endogenous RNAs (ceRNAs), regulate cellular processes under hypoxic conditions by interacting with microRNAs to influence cell differentiation, proliferation, apoptosis, and energy metabolism. However, the role of circRNAs in intracellular Ca<sup>2+</sup> homeostasis remains unclear. In this study, we explored the impact of the circAgtpbp1/rno-miR-30b-5p/Rgs8 axis on the regulation of Ca<sup>2+</sup> homeostasis in hypoxia-induced H9c2 cells, based on our previous findings related to anthocyanin-mediated hypoxia protection. We utilized various molecular tools, including circAgtpbp1 and Rgs8 overexpression vectors, si-RNA fragments, and rno-miR-30b-5p mimics and inhibitors, to modulate the expression of circAgtpbp1, Rgs8 and rno-miR-30b-5p. Our results indicate that inhibition of rno-miR-30b-5p, along with overexpression of circAgtpbp1 and Rgs8, disrupts the regulatory effects of anthocyanins on hypoxia-induced H9c2 cells, while promoting these effects conversely. Moreover, circAgtpbp1 and Rgs8 synergistically modulate the influence of anthocyanins on these cells, with rno-miR-30b-5p counteracting the effects of Rgs8 and circAgtpbp1. These findings suggest that anthocyanins regulate Ca<sup>2+</sup> homeostasis in hypoxia-induced H9c2 cells through the circAgtpbp1/Rgs8/rno-miR-30b-5p axis and provide insights into the molecular mechanisms underlying anthocyanin-mediated hypoxia protection, thus establishing a foundation for further research on cardiac protection in hypoxic conditions.</p>\u0000 </div>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2025 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/9916493","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144807520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
Journal of Food Biochemistry
全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1