Introduction: Oral cancer’s aggressive nature poses a significant health risk, demanding timely diagnosis and effective treatment. Despite progress in conventional therapies like chemotherapy and surgery, their limitations drive the exploration of alternative strategies. This study assessed Solanum trilobatum-derived silver nanoparticles (AgNPs) in impacting cancer cell cycles, inducing apoptosis, and modulating key pathways, including the phosphoinositide 3-kinase (PI3K)/Protein kinase B (AKT)/mammalian target of rapamycin (mTOR) pathway, leveraging phytotherapy and nanotechnology—a promising frontier in cancer treatment. Methods: AgNPs were synthesized through the reduction of ions and stabilized using aqueous leaf extracts of S. trilobatum. After the characterization of AgNPs, the mRNA Gene Expression and mitochondrial membrane potentials (MMPs) were assessed. DNA fragmentation was done and DNA pattern by gel documentation system was observed. The study also assessed the modulation of the PI3K/Akt/mTOR cascade, impacting tumor growth and proliferation. Results: Biosynthesized AgNPs were characterized using UV-visible spectrophotometry (UVvis), energy-dispersive X-ray spectroscopy (EDX), scanning electron microscopy (SEM), and Fourier-transform infrared (FTIR) spectroscopy. DNA fragmentation exhibited a typical ladder pattern. Dose-dependent changes in MMP were observed in the treated oral cancer cells. The effect of S. trilobatum-derived AgNPs in targeting the cell signaling pathway correlated significantly with their anticancer potency (P<0.001). Conclusion: This study reveals S. trilobatum leaf extract-based AgNPs as a natural cytostatic agent against oral squamous carcinoma. Utilizing nature’s resources and nanoscale science, they hold promise for enhancing oral cancer treatment outcomes and survival rates.
口腔癌口腔癌具有侵袭性，对健康构成重大威胁，需要及时诊断和有效治疗。尽管化疗和手术等传统疗法取得了进展，但其局限性促使人们探索替代策略。本研究评估了三叶茄提取的银纳米粒子（AgNPs）在影响癌细胞周期、诱导细胞凋亡和调节关键通路（包括磷脂酰肌醇3-激酶（PI3K）/蛋白激酶B（AKT）/哺乳动物雷帕霉素靶标（mTOR）通路）方面的作用，充分利用了植物疗法和纳米技术--这是癌症治疗领域前景广阔的前沿技术。方法：通过还原离子合成 AgNPs，并使用三叶草的水性叶提取物进行稳定。AgNPs 表征后，评估了 mRNA 基因表达和线粒体膜电位（MMPs）。通过凝胶记录系统进行了 DNA 断裂和 DNA 模式观察。研究还评估了对 PI3K/Akt/mTOR 级联反应的调节，以及对肿瘤生长和增殖的影响。研究结果使用紫外可见分光光度法（UVvis）、能量色散 X 射线光谱法（EDX）、扫描电子显微镜（SEM）和傅立叶变换红外光谱法（FTIR）对生物合成的 AgNPs 进行了表征。DNA 断裂呈现典型的梯形模式。在经处理的口腔癌细胞中观察到了 MMP 的剂量依赖性变化。S.trilobatum衍生的AgNPs靶向细胞信号通路的效果与其抗癌效力显著相关（P<0.001）。结论本研究揭示了以三叶木通叶提取物为基础的 AgNPs 是一种抗口腔鳞癌的天然细胞抑制剂。利用大自然的资源和纳米科学，它们有望提高口腔癌的治疗效果和生存率。
{"title":"Solanum trilobatum leaf extract-derived silver nanoparticles downregulate the PI3K/AKT/mTOR signaling pathway and attenuate oral squamous cell carcinoma cell proliferation","authors":"Anuradha Ganesan, Gautham Kumar N, P. Natarajan","doi":"10.34172/jhp.2024.49406","DOIUrl":"https://doi.org/10.34172/jhp.2024.49406","url":null,"abstract":"Introduction: Oral cancer’s aggressive nature poses a significant health risk, demanding timely diagnosis and effective treatment. Despite progress in conventional therapies like chemotherapy and surgery, their limitations drive the exploration of alternative strategies. This study assessed Solanum trilobatum-derived silver nanoparticles (AgNPs) in impacting cancer cell cycles, inducing apoptosis, and modulating key pathways, including the phosphoinositide 3-kinase (PI3K)/Protein kinase B (AKT)/mammalian target of rapamycin (mTOR) pathway, leveraging phytotherapy and nanotechnology—a promising frontier in cancer treatment. Methods: AgNPs were synthesized through the reduction of ions and stabilized using aqueous leaf extracts of S. trilobatum. After the characterization of AgNPs, the mRNA Gene Expression and mitochondrial membrane potentials (MMPs) were assessed. DNA fragmentation was done and DNA pattern by gel documentation system was observed. The study also assessed the modulation of the PI3K/Akt/mTOR cascade, impacting tumor growth and proliferation. Results: Biosynthesized AgNPs were characterized using UV-visible spectrophotometry (UVvis), energy-dispersive X-ray spectroscopy (EDX), scanning electron microscopy (SEM), and Fourier-transform infrared (FTIR) spectroscopy. DNA fragmentation exhibited a typical ladder pattern. Dose-dependent changes in MMP were observed in the treated oral cancer cells. The effect of S. trilobatum-derived AgNPs in targeting the cell signaling pathway correlated significantly with their anticancer potency (P<0.001). Conclusion: This study reveals S. trilobatum leaf extract-based AgNPs as a natural cytostatic agent against oral squamous carcinoma. Utilizing nature’s resources and nanoscale science, they hold promise for enhancing oral cancer treatment outcomes and survival rates.","PeriodicalId":15934,"journal":{"name":"Journal of HerbMed Pharmacology","volume":"282 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140766132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
W. BÉLEM-KABRÉ, Ollo Da, Ernest Nogma Sombié, Rainatou Boly, G. Ouédraogo, W. Ouédraogo, Zakaline Yabré, Jean Noel Dado Koussé, L. Belemnaba, A. Lamien-Sanou, N. Ouédraogo
Introduction: Ampelocissus africana is a medicinal plant used to treat several diseases, including those affecting the prostate. This study investigated the effects of the aqueous extract of rhizomes from Ampelocissus africana (AAA) on a testosterone propionate (TP)-induced benign prostatic hyperplasia (BPH) model. Methods: The effect of the extract at 50, 150, and 300 mg/kg on BPH in male rats was assessed using daily subcutaneous injections of 3 mg/kg TP for four consecutive weeks. After the induction schedule, physical parameters, prostate index (PI), serum biochemical parameters, homogenate antioxidant, enzyme parameters, and prostates histopathological studies were carried out. Triterpenes and sterols were also assessed. Results: Treatment with AAA reduced the PI level in a dose-dependent manner (up to 75.91% at the dose of 300 mg/kg). The inflammatory marker measured in the present study, namely C-reactive protein, increased significantly in the BPH group and improved with the treatments. In addition, AAA reduced oxidative stress by decreasing the malondialdehyde (MDA) level and increasing the catalase (CAT) and superoxide dismutase (SOD) levels in comparison to the positive control group. A histopathological examination corroborated the result of the analysis of the physical and biochemical parameters. The study also showed interesting levels of triterpenes (119.5 ± 4.5 mg UAE/g) and sterols (104.6 ± 3.06 mg CE/g). Conclusion: These results suggest that the aqueous extract of Ampelocissus africana could be used as a natural herbal therapy to treat BPH.
{"title":"Therapeutic effect of aqueous extract from Ampelocissus africana (Lour) Merr rhizomes on testosterone-induced benign prostatic hyperplasia in Wistar rats","authors":"W. BÉLEM-KABRÉ, Ollo Da, Ernest Nogma Sombié, Rainatou Boly, G. Ouédraogo, W. Ouédraogo, Zakaline Yabré, Jean Noel Dado Koussé, L. Belemnaba, A. Lamien-Sanou, N. Ouédraogo","doi":"10.34172/jhp.2024.42388","DOIUrl":"https://doi.org/10.34172/jhp.2024.42388","url":null,"abstract":"Introduction: Ampelocissus africana is a medicinal plant used to treat several diseases, including those affecting the prostate. This study investigated the effects of the aqueous extract of rhizomes from Ampelocissus africana (AAA) on a testosterone propionate (TP)-induced benign prostatic hyperplasia (BPH) model. Methods: The effect of the extract at 50, 150, and 300 mg/kg on BPH in male rats was assessed using daily subcutaneous injections of 3 mg/kg TP for four consecutive weeks. After the induction schedule, physical parameters, prostate index (PI), serum biochemical parameters, homogenate antioxidant, enzyme parameters, and prostates histopathological studies were carried out. Triterpenes and sterols were also assessed. Results: Treatment with AAA reduced the PI level in a dose-dependent manner (up to 75.91% at the dose of 300 mg/kg). The inflammatory marker measured in the present study, namely C-reactive protein, increased significantly in the BPH group and improved with the treatments. In addition, AAA reduced oxidative stress by decreasing the malondialdehyde (MDA) level and increasing the catalase (CAT) and superoxide dismutase (SOD) levels in comparison to the positive control group. A histopathological examination corroborated the result of the analysis of the physical and biochemical parameters. The study also showed interesting levels of triterpenes (119.5 ± 4.5 mg UAE/g) and sterols (104.6 ± 3.06 mg CE/g). Conclusion: These results suggest that the aqueous extract of Ampelocissus africana could be used as a natural herbal therapy to treat BPH.","PeriodicalId":15934,"journal":{"name":"Journal of HerbMed Pharmacology","volume":"204 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140775955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Adepoju, M. Ntwasa, S. Lebelo, T. Oyedepo, A. Ayeleso
Introduction: Metabolic syndrome is multifaceted health condition associated with metabolism, which leads to the accumulation of oxidation products and impairment of the antioxidant system. Medicinal, edible plant alternatives are being sought to prevent the predisposing factors of metabolic syndrome, one of which is obesity. We studied the effects of hydroethanolic garlic extract (HGE) on oxidative stress, lipolysis, and glycogenesis in high-fat diet-fed Drosophila melanogaster. Methods: The HGE was processed and antioxidant assays (in vitro and in vivo) coupled with metabolic biomarkers were assayed following standard procedures. Results: HGE inhibited ABTS (2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), hydroxyl, and hydrogen peroxide radicals in high-fat-fed D. melanogaster. The HGE also reduced the activity of superoxide dismutase and increased catalase activity significantly. The HGE promoted lipolysis and glycogenesis by reducing cholesterol, triglycerides, and total sugar concentrations while glycogen content was increased. Conclusion: Our results revealed that HGE improved the antioxidant defense system and promoted lipolysis and glycogenesis in metabolic deranged D. melanogaster. This suggests the therapeutic usefulness of garlic in the management of metabolic diseases.
{"title":"Effects of hydroethanolic garlic extract on oxidative stress, lipolysis, and glycogenesis in high-fat diet-fed Drosophila melanogaster","authors":"A. Adepoju, M. Ntwasa, S. Lebelo, T. Oyedepo, A. Ayeleso","doi":"10.34172/jhp.2024.46051","DOIUrl":"https://doi.org/10.34172/jhp.2024.46051","url":null,"abstract":"Introduction: Metabolic syndrome is multifaceted health condition associated with metabolism, which leads to the accumulation of oxidation products and impairment of the antioxidant system. Medicinal, edible plant alternatives are being sought to prevent the predisposing factors of metabolic syndrome, one of which is obesity. We studied the effects of hydroethanolic garlic extract (HGE) on oxidative stress, lipolysis, and glycogenesis in high-fat diet-fed Drosophila melanogaster. Methods: The HGE was processed and antioxidant assays (in vitro and in vivo) coupled with metabolic biomarkers were assayed following standard procedures. Results: HGE inhibited ABTS (2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), hydroxyl, and hydrogen peroxide radicals in high-fat-fed D. melanogaster. The HGE also reduced the activity of superoxide dismutase and increased catalase activity significantly. The HGE promoted lipolysis and glycogenesis by reducing cholesterol, triglycerides, and total sugar concentrations while glycogen content was increased. Conclusion: Our results revealed that HGE improved the antioxidant defense system and promoted lipolysis and glycogenesis in metabolic deranged D. melanogaster. This suggests the therapeutic usefulness of garlic in the management of metabolic diseases.","PeriodicalId":15934,"journal":{"name":"Journal of HerbMed Pharmacology","volume":"38 12","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140771387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Introduction: Olax subscorpioidea is a medicinal plant that Africans use to treat numerous ailments, including cancer. This research examines the antioxidant, anticancer, and in-silico properties of ethyl acetate fraction of Olax subscorpioidea’s (OSEA) on 7,12-Dimethylbenz(α) anthracene (DMBA)-induced cell proliferation in female rats. Methods: Forty female Sprague Dawley rats averaging 110 ± 20 g were induced proliferation with DMBA (80 mg/kg) and treated with ethyl acetate fraction (250 mg/kg BW) of O. subscorpioidea or tamoxifen (6.6 mg/kg BW) before and after induction. The trial lasted 22 weeks. In-vivo antioxidant parameters such as superoxide dismutase (SOD), malondialdehyde (MDA), and reduced glutathione (GSH) were examined. Likewise, carcinoma antigen marker (CA153), and DNA methyltransferase 3-like (DNMT3L) activity were measured. Gas chromatography-mass spectrometry (GC-MS) detected the bioactive compounds, and molecular docking studies predicted the mechanism of action of OSEA against DNA methyltransferase. Results: Treatment with OSEA significantly increased the SOD activity, enhanced GSH levels, and lowered the levels of MDA, CA-153, and DNMT3L in DMBA-exposed rats. The GC-MS analysis of OSEA revealed the presence of 40 bioactive compounds. The molecular docking revealed that 4-cyclopentene-1,3-dione (-6.407 kcal/mol), 2-(2-hydroxyethylthio) (-4.926 kcal/mol) and 3,4,5,6-tetrahydrophthalic anhydride (-6.16 kcal/mol) had the lowest binding energies against DNMT1, DNMT3A, and DNMT3B, respectively. 2-(2-hydroxyethylthio) was the least toxic. The molecular dynamic simulation revealed that the interaction between DNMT3A and 2-(2-hydroxyethylthio) propionic was stable to an extent. : The in-silico and biochemical analysis of the ethyl acetate fraction of O. subscorpioidea showed that it can protect against lipid peroxidation and oxidative stress and may be a potent source of drug that serves as an effective therapeutic in the future.
{"title":"In-silico and biochemical analysis of ethyl acetate fraction of Olax subscorpioidea leaf on DMBA-induced cell proliferation in female rats","authors":"A. A. Adelegan, T. Johnson, T. Dokunmu, E. Iweala","doi":"10.34172/jhp.2024.48154","DOIUrl":"https://doi.org/10.34172/jhp.2024.48154","url":null,"abstract":"Introduction: Olax subscorpioidea is a medicinal plant that Africans use to treat numerous ailments, including cancer. This research examines the antioxidant, anticancer, and in-silico properties of ethyl acetate fraction of Olax subscorpioidea’s (OSEA) on 7,12-Dimethylbenz(α) anthracene (DMBA)-induced cell proliferation in female rats. Methods: Forty female Sprague Dawley rats averaging 110 ± 20 g were induced proliferation with DMBA (80 mg/kg) and treated with ethyl acetate fraction (250 mg/kg BW) of O. subscorpioidea or tamoxifen (6.6 mg/kg BW) before and after induction. The trial lasted 22 weeks. In-vivo antioxidant parameters such as superoxide dismutase (SOD), malondialdehyde (MDA), and reduced glutathione (GSH) were examined. Likewise, carcinoma antigen marker (CA153), and DNA methyltransferase 3-like (DNMT3L) activity were measured. Gas chromatography-mass spectrometry (GC-MS) detected the bioactive compounds, and molecular docking studies predicted the mechanism of action of OSEA against DNA methyltransferase. Results: Treatment with OSEA significantly increased the SOD activity, enhanced GSH levels, and lowered the levels of MDA, CA-153, and DNMT3L in DMBA-exposed rats. The GC-MS analysis of OSEA revealed the presence of 40 bioactive compounds. The molecular docking revealed that 4-cyclopentene-1,3-dione (-6.407 kcal/mol), 2-(2-hydroxyethylthio) (-4.926 kcal/mol) and 3,4,5,6-tetrahydrophthalic anhydride (-6.16 kcal/mol) had the lowest binding energies against DNMT1, DNMT3A, and DNMT3B, respectively. 2-(2-hydroxyethylthio) was the least toxic. The molecular dynamic simulation revealed that the interaction between DNMT3A and 2-(2-hydroxyethylthio) propionic was stable to an extent. : The in-silico and biochemical analysis of the ethyl acetate fraction of O. subscorpioidea showed that it can protect against lipid peroxidation and oxidative stress and may be a potent source of drug that serves as an effective therapeutic in the future.","PeriodicalId":15934,"journal":{"name":"Journal of HerbMed Pharmacology","volume":"265 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140779879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Maya Hudiyati, Siti Sunarintyas, Retno Ardhani, A. Isnansetyo
Fucoidan, produced by the cell walls of brown seaweed, possesses biological effects, covering anticancer, antiviral, anti-inflammatory, antioxidant, and a potential for promoting angiogenesis and osteogenesis. This study aimed to compile diverse scientific results on fucoidan’s therapeutic prospect in dentistry and the mechanism of action in the therapy of oral diseases. A literature search was carried out using keywords with Boolean operators, including fucoidan (AND) oral (OR) dental (OR) dentistry, to identify related publications from PubMed, ScienceDirect, and Google Scholar databases. The results showed that fucoidan had various therapeutic potentials in the field of dentistry, including anticancer, anti-inflammatory, radioprotection, protection of dental pulp tissue, and bone regeneration. These characteristics were related to the sulfate composition and molecular weight. Fucoidan has various therapeutic potentials crucial for oral health. Hence, it might be used for material development and drug production in dentistry
{"title":"Therapeutic potential of fucoidan in dentistry: A review","authors":"Maya Hudiyati, Siti Sunarintyas, Retno Ardhani, A. Isnansetyo","doi":"10.34172/jhp.2024.48302","DOIUrl":"https://doi.org/10.34172/jhp.2024.48302","url":null,"abstract":"Fucoidan, produced by the cell walls of brown seaweed, possesses biological effects, covering anticancer, antiviral, anti-inflammatory, antioxidant, and a potential for promoting angiogenesis and osteogenesis. This study aimed to compile diverse scientific results on fucoidan’s therapeutic prospect in dentistry and the mechanism of action in the therapy of oral diseases. A literature search was carried out using keywords with Boolean operators, including fucoidan (AND) oral (OR) dental (OR) dentistry, to identify related publications from PubMed, ScienceDirect, and Google Scholar databases. The results showed that fucoidan had various therapeutic potentials in the field of dentistry, including anticancer, anti-inflammatory, radioprotection, protection of dental pulp tissue, and bone regeneration. These characteristics were related to the sulfate composition and molecular weight. Fucoidan has various therapeutic potentials crucial for oral health. Hence, it might be used for material development and drug production in dentistry","PeriodicalId":15934,"journal":{"name":"Journal of HerbMed Pharmacology","volume":"119 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140790782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Dermane, Atsu Kodjo George Kporvie, Kpoyizoun Pascaline Kindji, K. Metowogo, K. Eklu-Gadegbeku
Introduction: The use of Securidaca longipedunculata for solving health problems related to immunological disorders is long-standing. The immunomodulatory activity associated with the anti-inflammatory effect of S. longipedunculata has not yet been elucidated by scientific research. The aim of our study is to show that the leaves of S. longipedunculata can solve problems related to immunodeficiency and inflammation. Methods: The phytochemical compounds of the plant were carried out by solubility tests. The anti-inflammatory activity of the plant was evaluated by the chicken egg albumin denaturation inhibition, membrane stabilization, and C-reactive protein (CRP) tests. The 2,2-diphenyl1-picrylhydrazyl, total antioxidant capacity, and inhibition of lipoperoxidation tests were performed to evaluate the antioxidant activity of the plant. Immunosuppression was induced in rats by cyclophosphamide. The immunomodulatory activity of S. longipedunculata was studied by blood count. The lactate dehydrogenase (LDH) titration showed the effect of S. longipedunculata on the energy balance. Results: The extract contained polyphenols, flavonoids, and tannins. The tests of albumin denaturation inhibition, membrane stabilisation, CRP, total antioxidant capacity, and 2,2-diphenyl-1-picrylhydrazyl revealed that the extract had anti-inflammatory and antioxidant effects. The blood count results revealed that the extract non-significantly increased the number of leukocytes except in the case of neutrophils and monocytes at a dose of 400 mg/kg (P<0.05). The extract also restored the energy balance in the rats according to the LDH results. Conclusion: This study reveals that the hydro-ethanolic extract of S. longipedunculata leaves has potential immunostimulant and anti-inflammatory effects.
简介长期以来，人们一直使用长叶山龙眼（Securidaca longipedunculata）来解决与免疫紊乱有关的健康问题。科学研究尚未阐明与长叶蛇莓抗炎作用相关的免疫调节活性。我们研究的目的是证明长叶木贼的叶子可以解决与免疫缺陷和炎症有关的问题。研究方法通过溶解度测试对该植物的植物化学成分进行了研究。通过鸡卵白蛋白变性抑制试验、膜稳定试验和 C 反应蛋白（CRP）试验评估了该植物的抗炎活性。此外，还进行了 2,2-二苯基-1-苦基肼、总抗氧化能力和抑制脂肪过氧化试验，以评估该植物的抗氧化活性。环磷酰胺诱导大鼠免疫抑制。通过血液计数研究了长叶杉木的免疫调节活性。乳酸脱氢酶（LDH）滴定法显示了龙舌兰对能量平衡的影响。研究结果提取物中含有多酚、类黄酮和单宁酸。对白蛋白变性抑制、膜稳定性、CRP、总抗氧化能力和 2,2-二苯基-1-苦基肼的测试表明，该提取物具有抗炎和抗氧化作用。血细胞计数结果显示，在剂量为 400 毫克/千克时，除中性粒细胞和单核细胞外，提取物对白细胞数量的增加无显著影响（P<0.05）。根据 LDH 结果，提取物还能恢复大鼠的能量平衡。结论本研究揭示了 S. longipedunculata 叶子的水乙醇提取物具有潜在的免疫刺激和抗炎作用。
{"title":"Immunomodulatory and anti-inflammatory activities of hydro-ethanolic extract of Securidaca longipedunculata Fresen leaves","authors":"A. Dermane, Atsu Kodjo George Kporvie, Kpoyizoun Pascaline Kindji, K. Metowogo, K. Eklu-Gadegbeku","doi":"10.34172/jhp.2024.49352","DOIUrl":"https://doi.org/10.34172/jhp.2024.49352","url":null,"abstract":"Introduction: The use of Securidaca longipedunculata for solving health problems related to immunological disorders is long-standing. The immunomodulatory activity associated with the anti-inflammatory effect of S. longipedunculata has not yet been elucidated by scientific research. The aim of our study is to show that the leaves of S. longipedunculata can solve problems related to immunodeficiency and inflammation. Methods: The phytochemical compounds of the plant were carried out by solubility tests. The anti-inflammatory activity of the plant was evaluated by the chicken egg albumin denaturation inhibition, membrane stabilization, and C-reactive protein (CRP) tests. The 2,2-diphenyl1-picrylhydrazyl, total antioxidant capacity, and inhibition of lipoperoxidation tests were performed to evaluate the antioxidant activity of the plant. Immunosuppression was induced in rats by cyclophosphamide. The immunomodulatory activity of S. longipedunculata was studied by blood count. The lactate dehydrogenase (LDH) titration showed the effect of S. longipedunculata on the energy balance. Results: The extract contained polyphenols, flavonoids, and tannins. The tests of albumin denaturation inhibition, membrane stabilisation, CRP, total antioxidant capacity, and 2,2-diphenyl-1-picrylhydrazyl revealed that the extract had anti-inflammatory and antioxidant effects. The blood count results revealed that the extract non-significantly increased the number of leukocytes except in the case of neutrophils and monocytes at a dose of 400 mg/kg (P<0.05). The extract also restored the energy balance in the rats according to the LDH results. Conclusion: This study reveals that the hydro-ethanolic extract of S. longipedunculata leaves has potential immunostimulant and anti-inflammatory effects.","PeriodicalId":15934,"journal":{"name":"Journal of HerbMed Pharmacology","volume":"246 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140792752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ahila Meliana, Arifian Hardi Putri Ratnani, Nurina Hasanatuludhhiyah, A. Rahniayu, Gondo Mastutik, A. S. Rahaju
Introduction: The liver and kidney are the main sites of aluminum (Al) accumulation. Lifetime exposure to significant amounts of Al is inevitable, hence its toxicity on the liver and kidney should be a health concern. Natural antioxidants have been proven to alleviate pathologies in various liver and kidney injuries. However, the effect of olive leaf extract (OLE) on Al-exposed animals is yet to be confirmed. This study aimed to investigate the OLE effect against AlCl3 chronic exposure in rats’ liver and kidneys. Methods:Thirty-two male Wistar rats were divided into four groups (n=8), including the control group, the AlCl3 group treated with 128 mg/kg AlCl3 solution, as well as AlCl3 +OLE50, and AlCl3 +OLE100 groups (Other than AlCl3 they received 50 and 100 mg/kg of OLE, respectively, 2 hours after AlCl3 administration). All treatments were given orally for 12 weeks. All groups were evaluated for liver and kidney histopathological features, then scoring was performed. Results: AlCl3 administrations produced histopathological lesions in the liver and kidney, indicated by increased liver necro-inflammatory grades, ballooning scores, and renal inflammatory cell infiltration (P<0.05). OLE100 mg/kg significantly reduced liver necroinflammatory grade, ballooning score, and kidney inflammatory cell infiltrations. The dose of 50 mg/kg also reduced these parameters (P<0.05), except for the liver necro-inflammatory grade. There was a significant correlation between OLE dose and liver necro-inflammatory grade and ballooning score amelioration. Conclusion: OLE ameliorates liver and kidney histopathological features induced by oral Al chronic exposure in a dose-dependent manner.
{"title":"Protective effect of olive leaf (Olea europaea L.) extract against chronic exposure of liver and kidney tissues of Wistar rats to aluminum chloride","authors":"Ahila Meliana, Arifian Hardi Putri Ratnani, Nurina Hasanatuludhhiyah, A. Rahniayu, Gondo Mastutik, A. S. Rahaju","doi":"10.34172/jhp.2024.49313","DOIUrl":"https://doi.org/10.34172/jhp.2024.49313","url":null,"abstract":"Introduction: The liver and kidney are the main sites of aluminum (Al) accumulation. Lifetime exposure to significant amounts of Al is inevitable, hence its toxicity on the liver and kidney should be a health concern. Natural antioxidants have been proven to alleviate pathologies in various liver and kidney injuries. However, the effect of olive leaf extract (OLE) on Al-exposed animals is yet to be confirmed. This study aimed to investigate the OLE effect against AlCl3 chronic exposure in rats’ liver and kidneys. Methods:Thirty-two male Wistar rats were divided into four groups (n=8), including the control group, the AlCl3 group treated with 128 mg/kg AlCl3 solution, as well as AlCl3 +OLE50, and AlCl3 +OLE100 groups (Other than AlCl3 they received 50 and 100 mg/kg of OLE, respectively, 2 hours after AlCl3 administration). All treatments were given orally for 12 weeks. All groups were evaluated for liver and kidney histopathological features, then scoring was performed. Results: AlCl3 administrations produced histopathological lesions in the liver and kidney, indicated by increased liver necro-inflammatory grades, ballooning scores, and renal inflammatory cell infiltration (P<0.05). OLE100 mg/kg significantly reduced liver necroinflammatory grade, ballooning score, and kidney inflammatory cell infiltrations. The dose of 50 mg/kg also reduced these parameters (P<0.05), except for the liver necro-inflammatory grade. There was a significant correlation between OLE dose and liver necro-inflammatory grade and ballooning score amelioration. Conclusion: OLE ameliorates liver and kidney histopathological features induced by oral Al chronic exposure in a dose-dependent manner.","PeriodicalId":15934,"journal":{"name":"Journal of HerbMed Pharmacology","volume":"58 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140794883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Vijayaragavan Haripriya, S. Mahalaxmi, S. Vidhya, Pranav Vanajassun Purushothaman
Introduction: Bioactive compounds from plants have potential antimicrobial activity. The aim of this in vitro study was to evaluate the antimicrobial efficacy of curcumin, allicin, gingerol and cinnamon compared to 4% sodium hypochlorite (NaOCl) against Enterococcus faecalis and its biofilm. Methods: The dry herbal compounds were diluted with dimethyl sulfoxide (DMSO). Antimicrobial activity was evaluated using agar diffusion test, minimum inhibitory concentration (MIC) assay, minimum bactericidal concentration (MBC) test, time kill study, and biofilm susceptibility assay. The zone of inhibition (ZOI) was determined using agar diffusion test on Muller Hinton (MH) agar plates. MIC was evaluated using the tube dilution method. Root canals of extracted human anterior teeth were instrumented, split into two halves, autoclaved, and incubated with brain heart infusion broth containing E. faecalis for 21 days to form a biofilm. The susceptibility of the biofilm to the test solutions was evaluated by counting bacterial colonies on MH agar. Results: NaOCl exhibited potent antimicrobial activity under all tested parameters. Allicin showed a significantly greater ZOI, while curcumin showed the least MIC among the tested herbal extracts (P<0.05). MBC varied widely among the groups with no significant difference between allicin and cinnamon (P>0.05). Gingerol and cinnamon were significantly superior to the other groups killing E. faecalis within 4-4.2 min (P<0.05). Curcumin, gingerol, and cinnamon were equally efficacious as NaOCl in completely eradicating E. faecalis biofilm (P>0.05). Conclusion: NaOCl emerged as the most efficacious antibacterial agent and all herbal extracts showed significant antibacterial activity against E. faecalis.
{"title":"Antibacterial efficacy of curcumin, allicin, gingerol and cinnamon against Enterococcus faecalis: An in vitro study","authors":"Vijayaragavan Haripriya, S. Mahalaxmi, S. Vidhya, Pranav Vanajassun Purushothaman","doi":"10.34172/jhp.2023.46064","DOIUrl":"https://doi.org/10.34172/jhp.2023.46064","url":null,"abstract":"Introduction: Bioactive compounds from plants have potential antimicrobial activity. The aim of this in vitro study was to evaluate the antimicrobial efficacy of curcumin, allicin, gingerol and cinnamon compared to 4% sodium hypochlorite (NaOCl) against Enterococcus faecalis and its biofilm. Methods: The dry herbal compounds were diluted with dimethyl sulfoxide (DMSO). Antimicrobial activity was evaluated using agar diffusion test, minimum inhibitory concentration (MIC) assay, minimum bactericidal concentration (MBC) test, time kill study, and biofilm susceptibility assay. The zone of inhibition (ZOI) was determined using agar diffusion test on Muller Hinton (MH) agar plates. MIC was evaluated using the tube dilution method. Root canals of extracted human anterior teeth were instrumented, split into two halves, autoclaved, and incubated with brain heart infusion broth containing E. faecalis for 21 days to form a biofilm. The susceptibility of the biofilm to the test solutions was evaluated by counting bacterial colonies on MH agar. Results: NaOCl exhibited potent antimicrobial activity under all tested parameters. Allicin showed a significantly greater ZOI, while curcumin showed the least MIC among the tested herbal extracts (P<0.05). MBC varied widely among the groups with no significant difference between allicin and cinnamon (P>0.05). Gingerol and cinnamon were significantly superior to the other groups killing E. faecalis within 4-4.2 min (P<0.05). Curcumin, gingerol, and cinnamon were equally efficacious as NaOCl in completely eradicating E. faecalis biofilm (P>0.05). Conclusion: NaOCl emerged as the most efficacious antibacterial agent and all herbal extracts showed significant antibacterial activity against E. faecalis.","PeriodicalId":15934,"journal":{"name":"Journal of HerbMed Pharmacology","volume":"14 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139351064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sarath Kumar, Kavitha Sanjeev, Nagarathinam Sundaramoorthy, S. Mahalaxmi
Introduction: Ethylenediaminetetraacetic acid (EDTA), a widely used chelating agent, compromises the bond strength of resin-based sealers when used as a final irrigating solution. Hence, the push-out bond strength of AH Plus, a resin-based sealer, was analyzed when gallic acid, "a chelator" was used as a last irrigating solution. Methods: Forty human single-rooted teeth were accessed and randomly assigned into 4 groups (n = 10): EA (17% EDTA), MA (7% maleic acid), GA (10% gallic acid), and SA (saline). After irrigation, the root canals were coated with AH Plus sealer and obturated using gutta-percha. The root canals were sectioned horizontally into coronal, middle, and apical thirds for evaluating the push-out bond strength. A universal testing machine with a compressive loading of 1 mm/min was employed to test the samples. Results: An increased push-out bond strength was noted with GA in coronal one-third compared to EA and SA but not significant compared to MA (P > 0.05). Similar observations were seen in the middle one-third; however, it was statistically significant compared to all other groups (P < 0.05). Also, the difference between the groups in the apical one-third was not significant. SA showed the lowest push-out bond strength than experimental groups, which was significant in all three sections (P < 0.05). Conclusion: The final rinse of 10% gallic acid increased the push-out bond strength of AH Plus to the root dentin in all thirds of the root canal. Hence, gallic acid 10% might be an effective alternative solution in place of synthetic chelators.
简介：乙二胺四乙酸（EDTA）是一种广泛使用的螯合剂，在用作最后冲洗溶液时会影响树脂封闭剂的粘接强度。因此，我们分析了在使用没食子酸（一种螯合剂）作为最后冲洗液时，树脂基封闭剂 AH Plus 的推出粘接强度。研究方法取 40 颗人类单根牙齿，随机分为 4 组（n = 10）：EA（17% EDTA）、MA（7% 马来酸）、GA（10% 没食子酸）和 SA（生理盐水）。灌洗后，在根管上涂抹 AH Plus 封闭剂，并使用古塔波卡堵塞根管。将根管水平分为冠状、中间和根尖三部分，以评估推出粘接强度。使用压缩负荷为 1 毫米/分钟的万能试验机对样品进行测试。结果：与 EA 和 SA 相比，GA 在冠状三分之一处的推出粘接强度有所提高，但与 MA 相比并不显著（P > 0.05）。在中间三分之一处也观察到类似的情况，但与所有其他组相比，差异有统计学意义（P < 0.05）。此外，各组在顶端三分之一处的差异也不显著。与实验组相比，SA 显示出最低的推出粘接强度，这在所有三个部分都有显著性差异（P < 0.05）。结论10% 没食子酸的最后冲洗提高了 AH Plus 在根管所有三分之一处与根牙本质的推出粘接强度。因此，10% 没食子酸可能是替代合成螯合剂的有效方法。
{"title":"Effect of gallic acid as a final irrigant on push-out bond strength of an epoxy resin sealer to root canal dentin","authors":"Sarath Kumar, Kavitha Sanjeev, Nagarathinam Sundaramoorthy, S. Mahalaxmi","doi":"10.34172/jhp.2023.44919","DOIUrl":"https://doi.org/10.34172/jhp.2023.44919","url":null,"abstract":"Introduction: Ethylenediaminetetraacetic acid (EDTA), a widely used chelating agent, compromises the bond strength of resin-based sealers when used as a final irrigating solution. Hence, the push-out bond strength of AH Plus, a resin-based sealer, was analyzed when gallic acid, \"a chelator\" was used as a last irrigating solution. Methods: Forty human single-rooted teeth were accessed and randomly assigned into 4 groups (n = 10): EA (17% EDTA), MA (7% maleic acid), GA (10% gallic acid), and SA (saline). After irrigation, the root canals were coated with AH Plus sealer and obturated using gutta-percha. The root canals were sectioned horizontally into coronal, middle, and apical thirds for evaluating the push-out bond strength. A universal testing machine with a compressive loading of 1 mm/min was employed to test the samples. Results: An increased push-out bond strength was noted with GA in coronal one-third compared to EA and SA but not significant compared to MA (P > 0.05). Similar observations were seen in the middle one-third; however, it was statistically significant compared to all other groups (P < 0.05). Also, the difference between the groups in the apical one-third was not significant. SA showed the lowest push-out bond strength than experimental groups, which was significant in all three sections (P < 0.05). Conclusion: The final rinse of 10% gallic acid increased the push-out bond strength of AH Plus to the root dentin in all thirds of the root canal. Hence, gallic acid 10% might be an effective alternative solution in place of synthetic chelators.","PeriodicalId":15934,"journal":{"name":"Journal of HerbMed Pharmacology","volume":"67 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139351065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Behzad Imani, Sahar Alizade, S. Khazaei, Pouran Hajian
Introduction: The occurrence of nausea and vomiting following anesthesia and surgery is a prevalent and distressing issue, ranking second only to pain. In this study, the effects of ginger and ondansetron in mitigating these symptoms in patients who underwent cesarean section surgery were compared. Methods: This double-blinded randomized clinical trial included 150 eligible patients who were randomly assigned to one of three groups: ginger, ondansetron, and control. The ginger group was given one 1000 mg ginger capsule, the ondansetron group was given one 16 mg ondansetron capsule, and the control group was given one placebo capsule. Participants took their designated capsules with 30 mL of water one hour before their scheduled surgery. Nausea intensity and vomiting frequency were assessed throughout the surgical procedure and at post-operation intervals of 0.5, 1, 2, and 4 hours. Results: Compared to the control group, the ginger group had significantly less severe nausea during the surgery (P=0.03) and one hour after surgery (P=0.01). The ginger group also had significantly fewer vomiting episodes during the surgery (P=0.007) and half an hour after surgery (P=0.001). There was no significant difference between the ginger and ondansetron groups regarding the severity of nausea and the number of vomiting (P>0.05). Conclusion: The administration of ginger was found to be successful in alleviating the severity of nausea and vomiting both during and after spinal anesthesia for cesarean section procedures. It could be a viable alternative to ondansetron.
{"title":"Effects of ginger and ondansetron on intra and postoperative nausea and vomiting in cesarean section under spinal anesthesia: A double-blinded randomized clinical trial","authors":"Behzad Imani, Sahar Alizade, S. Khazaei, Pouran Hajian","doi":"10.34172/jhp.2023.46050","DOIUrl":"https://doi.org/10.34172/jhp.2023.46050","url":null,"abstract":"Introduction: The occurrence of nausea and vomiting following anesthesia and surgery is a prevalent and distressing issue, ranking second only to pain. In this study, the effects of ginger and ondansetron in mitigating these symptoms in patients who underwent cesarean section surgery were compared. Methods: This double-blinded randomized clinical trial included 150 eligible patients who were randomly assigned to one of three groups: ginger, ondansetron, and control. The ginger group was given one 1000 mg ginger capsule, the ondansetron group was given one 16 mg ondansetron capsule, and the control group was given one placebo capsule. Participants took their designated capsules with 30 mL of water one hour before their scheduled surgery. Nausea intensity and vomiting frequency were assessed throughout the surgical procedure and at post-operation intervals of 0.5, 1, 2, and 4 hours. Results: Compared to the control group, the ginger group had significantly less severe nausea during the surgery (P=0.03) and one hour after surgery (P=0.01). The ginger group also had significantly fewer vomiting episodes during the surgery (P=0.007) and half an hour after surgery (P=0.001). There was no significant difference between the ginger and ondansetron groups regarding the severity of nausea and the number of vomiting (P>0.05). Conclusion: The administration of ginger was found to be successful in alleviating the severity of nausea and vomiting both during and after spinal anesthesia for cesarean section procedures. It could be a viable alternative to ondansetron.","PeriodicalId":15934,"journal":{"name":"Journal of HerbMed Pharmacology","volume":"20 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139351108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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