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Investigation of class 1 integrons and biofilm formation in multi-drug resistance uropathogenic Escherichia coli isolated from patients with urinary tract infection in Shohadaye Qom hospital, Iran 伊朗Shohadaye Qom医院尿路感染患者多药耐药尿路致病性大肠杆菌1类整合子及生物膜形成的调查
Pub Date : 2022-01-01 DOI: 10.4103/iahs.iahs_163_21
A. Khorshidi, N. Zadeh, A. Khaledi, G. Moosavi, Ali Shakerimoghaddam, Azade Matinpur
Aims: This study aimed to investigate class 1 integrons and biofilm formation in multi-drug resistance (MDR) Uropathogenic Escherichia coli (UPEC) isolated from patients with urinary tract infection (UTI). Materials and Methods: Three hundred and eighty positive cultures were collected from patients with UTI referred to Shohadaye Qom hospital from 2018 to 2019. Suitable tests were done to diagnose UPEC, and confirmed by usp gene polymerase chain reaction (PCR). Antibiotic susceptibility testing was performed using Kirby Bauer disk diffusion. Analysis of biofilm production was conducted using microtiter plate assay. Next, the presence of Class 1 integrons and dfr-17 gene was surveyed by PCR. Data analyzed using Chi-squared and Fisher's exact tests in SPSS software, P < 0.05 was considered statistically significant. Findings: In total, 166 isolates of UPEC were retrieved. Among them, 120 isolates were MDR. The highest resistance of MDRs was observed against ampicillin. Among MDRs, 71, 18, 15, and 16 isolates were negative, weak, moderate, and strong biofilm producers, respectively. Meanwhile, 47.5% of the isolates were positive for int-1 gene and 25.8% of the isolates were positive for dfr-17-gene cassette. Out of 57 int-1 positive MDRs, 15 isolates (26.3%) showed strong biofilm which indicated a significant correlation (P < 0.001). Furthermore, among 31 MDRs with the positive dfr-17, 8 isolates (25.8%) had strong biofilm which statistically was significant (P < 0.001). Conclusion: The present study reported a significant correlation between cassettes genes, Class 1 integrons, and biofilm formation with antibiotic resistance pattern. Hence, continuous screening for antibiotics resistance is vital for infection control and prevention.
目的:研究尿路感染(UTI)患者多药耐药(MDR)尿路致病性大肠杆菌(UPEC) 1类整合子及其生物膜的形成。材料与方法:收集2018 - 2019年在Shohadaye Qom医院转诊的UTI患者中380例阳性培养物。采用usp基因聚合酶链反应(PCR)证实了UPEC的诊断。采用Kirby Bauer纸片扩散法进行药敏试验。采用微滴板法分析生物膜的生成情况。接下来,通过PCR检测1类整合子和dfr-17基因的存在。数据在SPSS软件中采用χ 2检验和Fisher精确检验,P < 0.05为差异有统计学意义。结果:共检索到166株UPEC。其中120株为耐多药菌株。耐多药耐药性最高的是氨苄西林。在mdr中,71株、18株、15株和16株分别为阴性、弱、中等和强生物膜产生菌。同时,有47.5%的分离株int-1基因阳性,25.8%的分离株dfr-17基因盒阳性。57株int-1阳性mdr中,有15株(26.3%)呈现强生物膜,相关性显著(P < 0.001)。31株dfr-17阳性mdr中,8株(25.8%)具有强生物膜,差异有统计学意义(P < 0.001)。结论:本研究报道了cassettes基因、1类整合子和生物膜形成与抗生素耐药模式之间的显著相关性。因此,持续筛查抗生素耐药性对感染控制和预防至关重要。
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引用次数: 0
Causes of road accidents in northwestern iran in the period 2010–2018 2010-2018年伊朗西北部道路交通事故原因分析
Pub Date : 2022-01-01 DOI: 10.4103/iahs.iahs_175_21
H. Mehryar, N. Alizadeh, Fatemeh Rostampour, S. Paryab, Sattar Bab, O. Garkaz
Aims: Traffic accidents are one of the major causes of death and disability worldwide. The aim of this study was to determine the causes of road accidents in northwestern Iran in the period of 2010–2018. Materials and Methods: This cross-sectional study was performed on all road traffic accidents recorded by traffic police of West Azerbaijan Province during 2010–2018. Data were analyzed using descriptive statistics, Chi-square t-test, and time series by SPSS 16. Results: A total of 95,788 registered accidents were included in the study. Most of the accidents were in September with the frequency of 9960 cases (10.4%), in residential, office, and industrial regions 58,550 (56%), by cars and taxi 80,949 (66%), in collisions between a vehicle with a bicycle and a motorcycle 56,728 (58%), in front-to-rear and right-side crashes 49,714 (47%), in rural and main roads 59,855 (62%), in clean weather 73,887 (73%), and on Thursday 14891 (15%); the occurrence of traffic accidents showed a significant relationship with all of these variables (P = 0.001). Conclusions: Month of accident, type of accident, day of the week, location of accident, use of vehicle, type of collision, mode of collision, accident path, and weather were the effective factors contributing in the occurrence of the traffic accidents. It is suggested that, in addition to educating people regarding the prevention of traffic accidents, policymakers take steps to improve the safety and standardization of roads and increase the safety of vehicles.
目的:交通事故是全世界造成死亡和残疾的主要原因之一。本研究的目的是确定2010-2018年伊朗西北部道路交通事故的原因。材料和方法:本横断面研究对2010-2018年西阿塞拜疆省交警记录的所有道路交通事故进行了研究。数据分析采用描述性统计、卡方t检验和SPSS 16时间序列分析。结果:该研究共纳入95,788起登记事故。大部分事故发生在9月份,9960起(10.4%),其次是住宅、办公和工业区58,550起(56%),汽车和出租车80,949起(66%),自行车和摩托车碰撞56,728起(58%),前后和右侧碰撞49,714起(47%),农村和主要道路59,855起(62%),晴朗天气73,887起(73%),周四14891起(15%);交通事故的发生与这些变量均有显著相关(P = 0.001)。结论:事故月份、事故类型、事故发生日期、事故地点、车辆使用、碰撞类型、碰撞方式、事故路径、天气是影响交通事故发生的有效因素。建议决策者除了教育人们如何预防交通事故外,还应采取措施提高道路的安全性和标准化,并提高车辆的安全性。
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引用次数: 0
Evaluation effect of chlorhexidine and green tea mouthwash on saliva pH and plaque index 氯己定与绿茶漱口水对唾液pH值及菌斑指数的影响
Pub Date : 2022-01-01 DOI: 10.4103/iahs.iahs_201_21
Mahshad Soltanian, M. Barekatain, S. Farhad, Mina Etemad
Aims: Applying mouthwash has an important role in reducing caries when compared to other prevention methods. The purpose of this study is to compare saliva pH and plaque index before and after using chlorhexidine mouthwash and a mouthwash with green tea extract. Materials and Methods: Forty dental students were randomly assigned to two groups (chlorhexidine and green tea mouthwash). A week before research getting started and during that, the students were asked to use normal diet and Bass brushing method. Salivary pH and plaque index of the participants were measured before, 1 week, and also 2 weeks after the use of mouth washes. Then, the data were analyzed based on Friedman, Mann–Whitney, and repeated-measures ANOVA tests. Results: The mean pH was significantly different in the three times of 1 week prior, during, and 2 week after the treatment for the green tea group (P = 0.01). However, there was no significant difference in the chlorhexidine group (P = 0.211). The mean of new plaque in both chlorhexidine green tea groups was significant (P < 0.001). The mean of plaque formation at <48 h in chlorhexidine and in green tea was both significant (P < 0.001). Conclusion: The effect of chlorhexidine mouthwash and green tea on salivary pH and plaque index was the same in a week. However, the effect of green tea was more than that of chlorhexidine in a 3-week application.
目的:与其他预防方法相比,使用漱口水在减少龋齿方面具有重要作用。本研究的目的是比较使用氯己定漱口水和绿茶提取物漱口水前后的唾液pH值和菌斑指数。材料与方法:将40名牙科学生随机分为两组(氯己定组和绿茶漱口水组)。在研究开始前一周和研究期间,学生们被要求使用正常饮食和Bass刷牙方法。分别在使用漱口水前、1周和2周后测量受试者唾液pH值和菌斑指数。然后,根据Friedman, Mann-Whitney和重复测量ANOVA检验对数据进行分析。结果:绿茶组治疗前1周、治疗中、治疗后2周3次pH值差异均有统计学意义(P = 0.01)。而氯己定组差异无统计学意义(P = 0.211)。两组新斑块的平均值均有显著性差异(P < 0.001)。氯己定和绿茶在<48 h时的平均斑块形成量均显著(P < 0.001)。结论:洗必泰漱口水与绿茶对口腔口腔唾液pH值及牙菌斑指数的影响在一周内相同。然而,在3周的应用中,绿茶的效果比氯己定要好。
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引用次数: 0
Case series of sudden death in sports from the year 2004 to 2020 in the city of Kashan, Iran: An education prospective 2004 - 2020年伊朗卡尚市体育运动猝死病例系列:教育前瞻性
Pub Date : 2022-01-01 DOI: 10.4103/iahs.iahs_120_20
M. Sayyah, Faeze Tohiditabar, Mohammmad Pourabbasi
Aim: The aim of this study is to study the frequency of sudden death in sports from the year 2004–2020 in the city of Kashan, Iran. Materials and Methods: This case series research included fatal sports injuries from the years 2004 to year 2020. Anecdotal data were collected by interviewing different sports organizations and close relatives of the victims. Result: The result of descriptive analysis of collected data showed that 17 cases of sudden sports death occurred during the years 2004 until 2020. The highest frequency of the cases occurred in mountain claiming with 9 (52.9%), followed by swimming with 3 (17.6%), and handball with 2 (11.8%), horse riding 1 (5.9%), basketball 1 (5.9%), and martial arts 1 (5.9%). All the analysis was performed using the SPSS: pc version 21. Conclusion: Fatal sports injuries are rare; however, they occur incidentally due to poor knowledge and education of the sport participants. It was concluded that nearly all fatal incidents were preventable.
目的:本研究的目的是研究2004-2020年伊朗卡尚市体育运动中猝死的频率。材料和方法:本研究包括2004年至2020年的致命性运动损伤病例系列研究。通过采访不同的体育组织和受害者的近亲收集了轶事数据。结果:对收集的资料进行描述性分析,2004 ~ 2020年共发生17例运动猝死。以山地运动发病最多,为9例(52.9%),其次为游泳3例(17.6%),手球2例(11.8%),骑马1例(5.9%),篮球1例(5.9%),武术1例(5.9%)。所有分析均使用SPSS: pc version 21进行。结论:致死性运动损伤罕见;然而,由于运动参与者缺乏知识和教育,它们偶然发生。结论是,几乎所有的致命事故都是可以避免的。
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引用次数: 0
Ecology and species composition of scorpions (Arachnida, Scorpiones) in Masjed-Soleyman County, Southwestern Iran 伊朗西南部Masjed-Soleyman县蝎子(蛛形纲,蝎类)的生态学和种类组成
Pub Date : 2021-10-01 DOI: 10.4103/iahs.iahs_161_21
H. Kassiri, M. Yousefi
Aims: The current study aimed to study the scorpion ecology and fauna of Masjed-Soleyman County, southwestern Iran to manage scorpion sting-related problems during 2016–2017. Materials and Methods: Scorpions were collected from different localities of Masjed-Soleyman using rock-rolling, digging the ground, Ultra Violet flashlight, pouring water in holes, and ordinary flashlight methods. The collected scorpions were placed to 70% alcohol. Morphological studies were completed under a stereomicroscope. The scorpion species were identified using diagnostic valid key for the Iranian scorpions. Results: Out of the 318 collected scorpions, 10 species from three families, buthidae (71.1%), scorpionidae (2.2%), and hemiscorpiidae (26.7%) were identified. The most abundant species were Androctonus crassicauda (32.4%), Hemiscorpius lepturus (26.7%), and Mesobuthus phillipsii (21.4%). The females were the dominant sex, i.e., 1.7:1 (F/M) sex ratio. The highest number of scorpions were caught in August (27.7%). The highest abundant of scorpions were collected in summer (65.7%). Furthermore, the majority of specimens were hunted through of ultraviolet flashlight (44.4%). The most abundant scorpions were caught in animal husbandry (23.6%). Conclusion: Because treatment of the patients who received stings should be managed according to the kind of their venom and the scorpion species, it is important for planning treatment and prevention according to the species composition of scorpions of each region.
目的:本研究旨在研究伊朗西南部Masjed-Soleyman县的蝎子生态和区系,以管理2016-2017年与蝎子蜇伤有关的问题。材料与方法:在Masjed-Soleyman的不同地点采集蝎子,采用摇石、挖地、紫外线手电筒、洞内灌水和普通手电筒等方法。将收集的蝎子放入70%的酒精中。形态学研究在体视显微镜下完成。利用伊朗蝎子的诊断有效密钥对蝎子种类进行了鉴定。结果:共采集318只蝎类,鉴定出蝎科(71.1%)、蝎科(2.2%)、半蝎科(26.7%)3科10种。种类最多的是粗尾雄蚊(32.4%)、lepturus(26.7%)和菲利浦中buthus(21.4%)。雌性为优势性别,雌雄比为1.7:1。8月捕获蝎子最多(27.7%)。夏季蝎子数量最多(65.7%);此外,绝大多数标本是通过紫外线手电筒捕获的(44.4%)。捕获蝎子最多的是畜牧业(23.6%)。结论:蜇伤患者的治疗应根据其毒液种类和蝎子种类进行管理,应根据各地区蝎子的种类组成来规划治疗和预防。
{"title":"Ecology and species composition of scorpions (Arachnida, Scorpiones) in Masjed-Soleyman County, Southwestern Iran","authors":"H. Kassiri, M. Yousefi","doi":"10.4103/iahs.iahs_161_21","DOIUrl":"https://doi.org/10.4103/iahs.iahs_161_21","url":null,"abstract":"Aims: The current study aimed to study the scorpion ecology and fauna of Masjed-Soleyman County, southwestern Iran to manage scorpion sting-related problems during 2016–2017. Materials and Methods: Scorpions were collected from different localities of Masjed-Soleyman using rock-rolling, digging the ground, Ultra Violet flashlight, pouring water in holes, and ordinary flashlight methods. The collected scorpions were placed to 70% alcohol. Morphological studies were completed under a stereomicroscope. The scorpion species were identified using diagnostic valid key for the Iranian scorpions. Results: Out of the 318 collected scorpions, 10 species from three families, buthidae (71.1%), scorpionidae (2.2%), and hemiscorpiidae (26.7%) were identified. The most abundant species were Androctonus crassicauda (32.4%), Hemiscorpius lepturus (26.7%), and Mesobuthus phillipsii (21.4%). The females were the dominant sex, i.e., 1.7:1 (F/M) sex ratio. The highest number of scorpions were caught in August (27.7%). The highest abundant of scorpions were collected in summer (65.7%). Furthermore, the majority of specimens were hunted through of ultraviolet flashlight (44.4%). The most abundant scorpions were caught in animal husbandry (23.6%). Conclusion: Because treatment of the patients who received stings should be managed according to the kind of their venom and the scorpion species, it is important for planning treatment and prevention according to the species composition of scorpions of each region.","PeriodicalId":160562,"journal":{"name":"International Archives of Health Sciences","volume":"34 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130210433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Risk factors for mortality in hospitalized moderate-to-severe COVID-19 patients: A single-center retrospective study 住院中重度COVID-19患者死亡率的危险因素:一项单中心回顾性研究
Pub Date : 2021-10-01 DOI: 10.4103/iahs.iahs_66_21
Leyla Bahadorizadeh, S. Fard, A. Behnagh, Seyed Hashemi Kiapey, S. Saneii, S. Minaeian
Aims: The aim of this study was to determine the mortality risk factors of hospitalized moderate-to-severe coronavirus disease 2019 (COVID-19) patients in Tehran. Materials and Methods: We retrospectively evaluated the baseline characteristics and clinical and paraclinical parameters of 223 deceased and discharged patients who were hospitalized in Firoozabadi General Hospital between March 1 and April 1, 2020. Results: According to our multiple logistic regression model, advanced age (odds ratio [OR], 1.06; 95% confidence interval [CI], 1.02–1.09; P < 0.01), reduced oxygen saturation (OR, 0.92; 95% CI, 0.87–0.96; P < 0.01), and hypertension (OR, 2.59; 95% CI, 1.04–6.46; P = 0.04) can be perceived as independent risk factors for mortality. Conclusion: Our results suggest that patients with older age, lower oxygen saturation, and hypertension are predisposed to an increased risk of mortality. Thus, to lower the COVID-19 mortality rates, patients with these characteristics should be the primary targets for early treatment, vaccination, or monitoring strategies.
目的:本研究的目的是确定德黑兰中重度冠状病毒病2019 (COVID-19)住院患者的死亡危险因素。材料和方法:我们回顾性评估2020年3月1日至4月1日期间在Firoozabadi综合医院住院的223例死亡和出院患者的基线特征和临床及临床旁参数。结果:根据多元logistic回归模型,高龄患者(优势比[OR], 1.06;95%置信区间[CI], 1.02-1.09;P < 0.01),血氧饱和度降低(OR, 0.92;95% ci, 0.87-0.96;P < 0.01),高血压(OR, 2.59;95% ci, 1.04-6.46;P = 0.04)可被视为死亡率的独立危险因素。结论:我们的研究结果表明,年龄较大、血氧饱和度较低和高血压的患者易增加死亡风险。因此,为了降低COVID-19死亡率,具有这些特征的患者应成为早期治疗、疫苗接种或监测策略的主要目标。
{"title":"Risk factors for mortality in hospitalized moderate-to-severe COVID-19 patients: A single-center retrospective study","authors":"Leyla Bahadorizadeh, S. Fard, A. Behnagh, Seyed Hashemi Kiapey, S. Saneii, S. Minaeian","doi":"10.4103/iahs.iahs_66_21","DOIUrl":"https://doi.org/10.4103/iahs.iahs_66_21","url":null,"abstract":"Aims: The aim of this study was to determine the mortality risk factors of hospitalized moderate-to-severe coronavirus disease 2019 (COVID-19) patients in Tehran. Materials and Methods: We retrospectively evaluated the baseline characteristics and clinical and paraclinical parameters of 223 deceased and discharged patients who were hospitalized in Firoozabadi General Hospital between March 1 and April 1, 2020. Results: According to our multiple logistic regression model, advanced age (odds ratio [OR], 1.06; 95% confidence interval [CI], 1.02–1.09; P < 0.01), reduced oxygen saturation (OR, 0.92; 95% CI, 0.87–0.96; P < 0.01), and hypertension (OR, 2.59; 95% CI, 1.04–6.46; P = 0.04) can be perceived as independent risk factors for mortality. Conclusion: Our results suggest that patients with older age, lower oxygen saturation, and hypertension are predisposed to an increased risk of mortality. Thus, to lower the COVID-19 mortality rates, patients with these characteristics should be the primary targets for early treatment, vaccination, or monitoring strategies.","PeriodicalId":160562,"journal":{"name":"International Archives of Health Sciences","volume":"8 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128838942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Cultural competence in clinical nursing: A qualitative study 临床护理中的文化能力:一项定性研究
Pub Date : 2021-10-01 DOI: 10.4103/iahs.iahs_139_21
M. Asadizaker, A. Ebadi, Shahram Molavynejad, S. Yadollahi
Aims: Given the wide cultural diversity of patients in the current world, nurses need to have great cultural competence. This study aimed to explore the concept of cultural competence in Iranian nurses. Materials and Methods: This qualitative study was conducted in 2017–2018 using Graneheim and Lundman's approach to qualitative content analysis. A purposeful sample of eighteen clinical nurses was recruited with maximum variation from hospitals of Isfahan and Ahvaz, Iran. Data were collected through eighteen semistructured interviews and simultaneously analyzed through directed content analysis. Findings: Data analysis resulted in the formation of thirteen subcategories which were respectively grouped into the four categories of cultural cognition (cultural awareness, cultural knowledge, and cultural insight), cultural care intention (cultural encounter, cultural desire, and cultural eagerness), cultural flexibility (cultural attitude, cultural sensitivity, and resolving cultural conflicts), and cultural care skills (communication skills, cultural evaluation skills, behavioral skills, and skills to get feedback about cultural care). Conclusion: Iran has many ethnic and religious groups which have a variety of subcultures, so Iranian nurses need to be sensitive about cultural diversity in health-care settings and have the cultural competence to facilitate the delivery of quality culturally competent care to the patients of different cultures.
目的:鉴于当今世界患者的广泛文化多样性,护士需要有很强的文化能力。本研究旨在探讨伊朗护士文化能力的概念。材料和方法:本定性研究于2017-2018年进行,采用Graneheim和Lundman的定性内容分析方法。从伊朗伊斯法罕和阿瓦士的医院招募了18名临床护士,其中差异最大。数据通过18个半结构化访谈收集,同时通过定向内容分析进行分析。发现:通过数据分析,形成了13个子类别,分别分为文化认知(文化意识、文化知识和文化洞察力)、文化关怀意愿(文化遭遇、文化欲望和文化渴望)、文化灵活性(文化态度、文化敏感性和解决文化冲突)和文化关怀技能(沟通技能、文化评价技能、行为技能、行为技能)四大类。以及获得文化关怀反馈的技能)。结论:伊朗有许多种族和宗教群体,这些群体有各种各样的亚文化,因此伊朗护士需要对保健机构的文化多样性敏感,并具有文化能力,以促进向不同文化的患者提供高质量的文化上合格的护理。
{"title":"Cultural competence in clinical nursing: A qualitative study","authors":"M. Asadizaker, A. Ebadi, Shahram Molavynejad, S. Yadollahi","doi":"10.4103/iahs.iahs_139_21","DOIUrl":"https://doi.org/10.4103/iahs.iahs_139_21","url":null,"abstract":"Aims: Given the wide cultural diversity of patients in the current world, nurses need to have great cultural competence. This study aimed to explore the concept of cultural competence in Iranian nurses. Materials and Methods: This qualitative study was conducted in 2017–2018 using Graneheim and Lundman's approach to qualitative content analysis. A purposeful sample of eighteen clinical nurses was recruited with maximum variation from hospitals of Isfahan and Ahvaz, Iran. Data were collected through eighteen semistructured interviews and simultaneously analyzed through directed content analysis. Findings: Data analysis resulted in the formation of thirteen subcategories which were respectively grouped into the four categories of cultural cognition (cultural awareness, cultural knowledge, and cultural insight), cultural care intention (cultural encounter, cultural desire, and cultural eagerness), cultural flexibility (cultural attitude, cultural sensitivity, and resolving cultural conflicts), and cultural care skills (communication skills, cultural evaluation skills, behavioral skills, and skills to get feedback about cultural care). Conclusion: Iran has many ethnic and religious groups which have a variety of subcultures, so Iranian nurses need to be sensitive about cultural diversity in health-care settings and have the cultural competence to facilitate the delivery of quality culturally competent care to the patients of different cultures.","PeriodicalId":160562,"journal":{"name":"International Archives of Health Sciences","volume":"18 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134048218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Molecular identification and frequency of cyst-forming coccidia (Sarcocystis, Toxoplasma gondii, and Neospora caninum) in native slaughtered cattle in Kashan, Central Iran 伊朗中部卡尚地区本地屠宰牛中囊状球虫(肉囊虫、刚地弓形虫和犬新孢子虫)的分子鉴定和频率
Pub Date : 2021-10-01 DOI: 10.4103/iahs.iahs_186_21
Hossein Hooshyar, F. Chehrazi, M. Arbabi
Aim: Cattle is one of the main sources of food supply chain for humans in most countries. The present study aimed to identify the infection rates for Toxoplasma gondii, Neospora caninum, and Sarcocystis spp. by polymerase chain reaction (PCR) method in native slaughtered cattle in Kashan, central Iran. Materials and Methods: Totally, 159 diaphragm, esophagus, and muscle samples (53 samples of each) were collected from native beef cattle from Kashan slaughterhouse, central Iran. The genomic DNA was extracted, and PCR method was used separately for detection of N. caninum, Sarcocystis, and T. gondii species using specific primers. Finding: Sarcocystis was found in 84.9% of muscles, 83% of esophagus, and 84.9% of diaphragm samples. Mixed infection (Sarcocystis cruzi–Sarcocystis hominis) was the most common infection, followed by S. cruzi and S. hominis. Sarcocystis hirsuta was not detected in any samples. T. gondii was detected only in three (5.7%) out of the 53 muscle tissues samples of cattle. N. caninum was found in 18.9% of muscles, 24.5% of esophagus, and 28.3% of diaphragm samples. One of the cattle had coinfection to Neospora, Toxoplasma, and Sarcocystis in muscles simultaneously. There was no statistically significant difference between infection rates and age as well as sex in each organ. Conclusion: This study revealed a low prevalence rate of T. gondii, but a high prevalence of infection to N. caninum and S. cruzi or mixed infection of S. cruzi with S. hominis among slaughtered cattle. Prevention measures such as keeping away dogs from cattle grazing are recommended.
目的:牛是大多数国家人类食品供应链的主要来源之一。本研究旨在利用聚合酶链反应(PCR)方法对伊朗中部卡尚地区本地屠宰牛中刚地弓形虫、犬新孢子虫和肉囊虫的感染率进行鉴定。材料与方法:从伊朗中部Kashan屠宰场采集本土肉牛横膈膜、食道和肌肉样本共159份(各53份)。提取基因组DNA,利用特异引物分别采用PCR方法检测犬奈瑟菌、肉囊菌和弓形虫。结果:84.9%的肌肉、83%的食道和84.9%的横膈膜样本中发现了肌囊炎。混合感染(克氏肌囊虫-人肉囊虫)最为常见,其次是克氏和人肉囊虫。所有样本均未检出毛性肉囊菌。在53份牛肌肉组织样本中仅检出3份(5.7%)弓形虫。在18.9%的肌肉、24.5%的食道和28.3%的膈肌样本中发现了犬乳杆菌。其中1头牛肌肉同时感染新孢子虫、弓形虫和肌囊虫。各脏器感染率与年龄、性别无统计学差异。结论:刚地弓形虫的流行率较低,但在屠宰牛中,犬奈瑟菌和克氏球菌感染或克氏球菌与人奈瑟菌混合感染的流行率较高。建议采取预防措施,例如让狗远离牧场。
{"title":"Molecular identification and frequency of cyst-forming coccidia (Sarcocystis, Toxoplasma gondii, and Neospora caninum) in native slaughtered cattle in Kashan, Central Iran","authors":"Hossein Hooshyar, F. Chehrazi, M. Arbabi","doi":"10.4103/iahs.iahs_186_21","DOIUrl":"https://doi.org/10.4103/iahs.iahs_186_21","url":null,"abstract":"Aim: Cattle is one of the main sources of food supply chain for humans in most countries. The present study aimed to identify the infection rates for Toxoplasma gondii, Neospora caninum, and Sarcocystis spp. by polymerase chain reaction (PCR) method in native slaughtered cattle in Kashan, central Iran. Materials and Methods: Totally, 159 diaphragm, esophagus, and muscle samples (53 samples of each) were collected from native beef cattle from Kashan slaughterhouse, central Iran. The genomic DNA was extracted, and PCR method was used separately for detection of N. caninum, Sarcocystis, and T. gondii species using specific primers. Finding: Sarcocystis was found in 84.9% of muscles, 83% of esophagus, and 84.9% of diaphragm samples. Mixed infection (Sarcocystis cruzi–Sarcocystis hominis) was the most common infection, followed by S. cruzi and S. hominis. Sarcocystis hirsuta was not detected in any samples. T. gondii was detected only in three (5.7%) out of the 53 muscle tissues samples of cattle. N. caninum was found in 18.9% of muscles, 24.5% of esophagus, and 28.3% of diaphragm samples. One of the cattle had coinfection to Neospora, Toxoplasma, and Sarcocystis in muscles simultaneously. There was no statistically significant difference between infection rates and age as well as sex in each organ. Conclusion: This study revealed a low prevalence rate of T. gondii, but a high prevalence of infection to N. caninum and S. cruzi or mixed infection of S. cruzi with S. hominis among slaughtered cattle. Prevention measures such as keeping away dogs from cattle grazing are recommended.","PeriodicalId":160562,"journal":{"name":"International Archives of Health Sciences","volume":"3 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126943992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Evaluation of thallium levels in opioid substance users: A case–control study 评估阿片类物质使用者的铊水平:一项病例对照研究
Pub Date : 2021-10-01 DOI: 10.4103/iahs.iahs_109_21
R. Eghtesadi, Peyman Esmaili-Shahzade-Ali-Akbari, Fateme Shahmirzaei, Hamidreza Banafsheh, A. Ghaderi
Aims: Drug abuse is one of the major problems threatening human health. The presence of heavy metals (e.g., lead and thallium) in illicit drugs has raised concerns. This study was designed and performed to indicate the status of thallium in the urine of opioid users with symptoms similar to thallotoxicosis and compare them with the control group. Materials and Methods: This case–control study was conducted in Kashan with the participation of 200 people (including 100 people in the opioid user group and 100 people in the control group). Then, electrothermal atomic absorption spectrometry was used to determine the concentrations of urinary thallium. Findings: In the opioid group, the median (interquartile range) urinary thallium concentrations was 72/29 ± 49/33 μg/l, whereas in the control group, it was 5/57 ± 3/015 μg/l. There was a significant difference in the concentrations of urinary thallium between the opioid group and the control group. Furthermore, the prevalence of thallotoxicosis-related clinical symptoms was significantly higher in the opioid group with high urinary thallium concentrations compared with the control group (weakness, fatigue, paresthesia, ataxia, vertigo, memory deficits, tremor, aggressiveness, tinnitus, sweating, rashes, dry skin, constipation, nausea, vomiting, abdominal pain, and diarrhea). Conclusions: The results of the present study indicated that the levels of urinary thallium in patients using illicit opioids are significantly higher, which can be due to contamination of drugs with thallium.
目的:药物滥用是威胁人类健康的主要问题之一。非法药物中重金属(例如铅和铊)的存在引起了关注。本研究的设计和实施是为了表明症状与铊中毒相似的阿片类药物使用者尿液中铊的状况,并将其与对照组进行比较。材料与方法:本病例-对照研究在喀山进行,共200人参与,其中阿片类药物使用者组100人,对照组100人。采用电热原子吸收光谱法测定尿铊浓度。结果:阿片类药物组尿铊浓度中位数(四分位数差)为72/29±49/33 μg/l,对照组为5/57±3/015 μg/l。阿片类药物组与对照组尿铊浓度差异有统计学意义。此外,与对照组相比,尿铊浓度高的阿片类药物组铊中毒相关临床症状(虚弱、疲劳、感觉异常、共济失调、眩晕、记忆缺陷、震颤、攻击性、耳鸣、出汗、皮疹、皮肤干燥、便秘、恶心、呕吐、腹痛和腹泻)的发生率明显更高。结论:本研究结果表明,使用非法阿片类药物的患者尿铊水平明显较高,这可能是由于药物被铊污染所致。
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引用次数: 1
Usability of electronic death registration system: A case study of heuristic evaluation 电子死亡登记系统的可用性:启发式评估的案例研究
Pub Date : 2021-10-01 DOI: 10.4103/iahs.iahs_86_21
F. Jeddi, Razieh Farrahi, Sara Chopannejad
Aim: This study was conducted to identify gaps in the user interface design of Iran's Electronic Death Registration System (National Registry) using the heuristic method. Materials and Methods: Five independent evaluators assessed the user interface using the heuristic evaluation. Problems were reassessed in the presence of all evaluators, and similar cases were merged, and a single list of unique problems was eventually prepared. By re-evaluating the system, the evaluators determined and categorized the severity of the problem in five domains, including no problem (zero point), a cosmetic problem (1 point), a minor problem (2 points), a major problem (3 points), and a catastrophic problem (4 points). The data were then analyzed in an excel sheet using descriptive statistics. Findings: Forty-two unique problems were identified. The “recognition rather than recall” principle with ten problems (23.8% of all cases) had the most frequent problem, while the “help and documentation” principle with one problem (2.4% of all cases) had the least frequency but the highest mean severity (4). Conclusion: The results of the heuristic evaluation of the electronic death registration system indicated that a large portion of the problems in this system was classified under the minor category, which primarily indicates the good usability of this system. However, this system suffers from several usability problems. Therefore, it could be concluded that considering the national-level use of this system, it has problems that could be resolved through meeting system design principles and standards.
目的:本研究采用启发式方法确定伊朗电子死亡登记系统(国家登记处)用户界面设计中的差距。材料与方法:五名独立评估者采用启发式评估法对用户界面进行评估。在所有评价人员在场的情况下对问题进行了重新评估,并合并了类似的案例,最后编制了一份独特问题的单一清单。通过重新评估系统,评估人员确定并将问题的严重性分为五个领域,包括没有问题(零分),一个外观问题(1分),一个次要问题(2分),一个主要问题(3分)和一个灾难性问题(4分)。然后使用描述性统计在excel表格中对数据进行分析。发现:确定了42个独特的问题。“识别而不是回忆”原则有10个问题(占所有病例的23.8%)是最常见的问题,而“帮助和文档”原则有1个问题(占所有病例的2.4%)出现的频率最少,但平均严重程度最高(4)。对电子死亡登记系统的启发式评价结果表明,该系统存在的大部分问题属于次要问题,这主要表明该系统具有良好的可用性。然而,这个系统存在一些可用性问题。因此,可以得出结论,考虑到该系统在国家层面的使用,它存在一些问题,可以通过满足系统设计原则和标准来解决。
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引用次数: 0
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International Archives of Health Sciences
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