Pub Date : 2024-08-05DOI: 10.1016/j.jksus.2024.103388
Bread waste in Saudi Arabia is a significant environmental issue that has caused significant losses. Insects, specifically Tenebrio molitor larvae, offer potential bioconversion solutions for food waste. Larvae were fed diets comprising different proportions of leftover bread (LB) powder mixed with wheat bran (WB), and feeding trials were conducted over 50 days. Results indicated that including LB powder did not significantly affect larval survival, affirming its safety. The larvae fed the diets LB100, LB75WB25, LB50WB50, LB25WB75, and WB reached the final weights of 27.64, 95.27, 105.012, 98.74, and 67.64 mg. Similarly, the highest mean pupal weight, at 0.133 mg, was observed when larvae were reared on LB75WB25, while the lowest mean pupal weight, at 0.107 mg, was recorded on the WB diet. Protein content varied significantly among diets, with mixed diets exhibiting protein content ranging from 16.98 % to 55.26 %. The primary fatty acid found in mealworm oil was oleic acid (C18:9), which made up 54.11 % of the total fatty acid content for larvae raised on 75LB:25WB, 52.01 % for LB, and 46.942 % for WB, with LB incorporation positively impacting growth, pupal weight, protein content, and antioxidant properties. Utilizing LB powder highlights its potential for sustainable insect farming, addressing environmental concerns, and enhancing resource sustainability.
{"title":"Leftover bread as a potential feed additive: Impact on growth, fatty acid content, and antioxidant properties in Tenebrio molitor larvae","authors":"","doi":"10.1016/j.jksus.2024.103388","DOIUrl":"10.1016/j.jksus.2024.103388","url":null,"abstract":"<div><p>Bread waste in Saudi Arabia is a significant environmental issue that has caused significant losses. Insects, specifically <em>Tenebrio molitor</em> larvae, offer potential bioconversion solutions for food waste. Larvae were fed diets comprising different proportions of leftover bread (LB) powder mixed with wheat bran (WB), and feeding trials were conducted over 50 days. Results indicated that including LB powder did not significantly affect larval survival, affirming its safety. The larvae fed the diets LB100, LB75WB25, LB50WB50, LB25WB75, and WB reached the final weights of 27.64, 95.27, 105.012, 98.74, and 67.64 mg. Similarly, the highest mean pupal weight, at 0.133 mg, was observed when larvae were reared on LB75WB25, while the lowest mean pupal weight, at 0.107 mg, was recorded on the WB diet. Protein content varied significantly among diets, with mixed diets exhibiting protein content ranging from 16.98 % to 55.26 %. The primary fatty acid found in mealworm oil was oleic acid (C18:9), which made up 54.11 % of the total fatty acid content for larvae raised on 75LB:25WB, 52.01 % for LB, and 46.942 % for WB, with LB incorporation positively impacting growth, pupal weight, protein content, and antioxidant properties. Utilizing LB powder highlights its potential for sustainable insect farming, addressing environmental concerns, and enhancing resource sustainability.</p></div>","PeriodicalId":16205,"journal":{"name":"Journal of King Saud University - Science","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1018364724003008/pdfft?md5=87519671180498da99c315d0f36a19b7&pid=1-s2.0-S1018364724003008-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141948822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-08-05DOI: 10.1016/j.jksus.2024.103385
Background
Soil is an important resource for improving the quality of human well-being and creating sustainable environmental awareness. Therefore, it is imperative to protect the soil health. This study determined quality of the soils in the Araban plain, Türkiye using three different methods, i.e., principal component analysis (PCA), analytic hierarchy process (AHP), and expert opinion (EO). The major aim was to determine the most appropriate method for determining soil quality and recommending soil protection practices for improving quality.
Methods
This study was conducted on an area of approximately 25,000 ha. A total of 230 soil samples (7 pasture areas, 56 orchards, and 167 arable crops) were analyzed to determine key soil quality indicators. A minimum dataset consisting of parameters that best represent the soils of the region was created. Subsequently, PCA was used to predict soil quality with the optimal accuracy using the least amount of data. The EO was incorporated to validate the PCA results. The AHP was used to assign weights to the parameters. Indicators at all sampling points were converted to unitless scorers. These scores were then formulated as soil quality index (SQI) using the weighted summation method.
Results
Organic matter (OM) exerted the highest impact on soil quality. Significant variations in SQI values were observed among arable land, orchards land and pastureland (P < 0.01). Significant variations were recorded in SQI values of the assessment methods employed in this study. The lowest (0.552) and highest (0.829) SQI values were recorded for AHP and EO, respectively. Overall, streamlining indicators through PCA gave the most accurate result for SQI value. Subsequently, SQI determination should involve weighing with AHP, particularly towards EO.
Conclusion
The AHP was found to be the most appropriate method for evaluating soil quality in Araban. This integrated approach ensures a robust evaluation of soil quality, facilitating more accurate and nuanced insights for informed land management decisions. Overall, 92.61 % of the region’s soil had poor and medium soil quality. Therefore, increasing OM content and aggregate stability are suggested to improve soil quality in the study region.
{"title":"Soil quality assessment in the Araban plain across various land use types","authors":"","doi":"10.1016/j.jksus.2024.103385","DOIUrl":"10.1016/j.jksus.2024.103385","url":null,"abstract":"<div><h3>Background</h3><p>Soil is an important resource for improving the quality of human well-being and creating sustainable environmental awareness. Therefore, it is imperative to protect the soil health. This study determined quality of the soils in the Araban plain, Türkiye using three different methods, i.e., principal component analysis (PCA), analytic hierarchy process (AHP), and expert opinion (EO). The major aim was to determine the most appropriate method for determining soil quality and recommending soil protection practices for improving quality.</p></div><div><h3>Methods</h3><p>This study was conducted on an area of approximately 25,000 ha. A total of 230 soil samples (7 pasture areas, 56 orchards, and 167 arable crops) were analyzed to determine key soil quality indicators. A minimum dataset consisting of parameters that best represent the soils of the region was created. Subsequently, PCA was used to predict soil quality with the optimal accuracy using the least amount of data. The EO was incorporated to validate the PCA results. The AHP was used to assign weights to the parameters. Indicators at all sampling points were converted to unitless scorers. These scores were then formulated as soil quality index (SQI) using the weighted summation method.</p></div><div><h3>Results</h3><p>Organic matter (OM) exerted the highest impact on soil quality. Significant variations in SQI values were observed among arable land, orchards land and pastureland (P < 0.01). Significant variations were recorded in SQI values of the assessment methods employed in this study. The lowest (0.552) and highest (0.829) SQI values were recorded for AHP and EO, respectively. Overall, streamlining indicators through PCA gave the most accurate result for SQI value. Subsequently, SQI determination should involve weighing with AHP, particularly towards EO.</p></div><div><h3>Conclusion</h3><p>The AHP was found to be the most appropriate method for evaluating soil quality in Araban. This integrated approach ensures a robust evaluation of soil quality, facilitating more accurate and nuanced insights for informed land management decisions. Overall, 92.61 % of the region’s soil had poor and medium soil quality. Therefore, increasing OM content and aggregate stability are suggested to improve soil quality in the study region.</p></div>","PeriodicalId":16205,"journal":{"name":"Journal of King Saud University - Science","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1018364724002970/pdfft?md5=033a61728d5c64316b535ecf80e1a9f5&pid=1-s2.0-S1018364724002970-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141948829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-08-03DOI: 10.1016/j.jksus.2024.103383
Objectives.
In silico techniques are a highly efficient, cost-effective, and rapid approach to identify potent herbal lead compounds with therapeutic potential against cancer. Phytochemicals are abundant in plants and may represent a promising cancer prevention and treatment approach. The plant species Oxalis corniculata has long been employed in traditional medicine to treat various diseases. Indeed, previous studies have demonstrated the anti-cancer properties of the crude extract obtained from this plant. However, further research is essential to elucidate precisely the underlying molecular mechanisms responsible for these activities.
Methods.
The study investigated the potential of bioactive compounds from O. corniculata to bind with different targets of anti-cancer drugs. Employing molecular docking and drug-likeness, 19 bioactive compounds from the plant were tested as potential anti-cancer leads. Compounds that exhibit both favorable drug-likeness and binding energies comparable to those of standard drugs like curcumin, doxorubicin, and paclitaxel were selected for further evaluation of their pharmacokinetic properties.
Results and Conclusions.
Among the 19 selected phytoconstituents of O. corniculata, compounds 15 and 16 exhibited the most effective binding energy (−8.68, −8.22 and −8.70, −8.22, −8.52 kcal/mol, respectively) with key cancer targets including Programmed death-ligand 1 (PDB: 7bea), B-Raf proto-oncogene, serine/threonine kinase (PDB: 8c7y) and poly (ADP-ribose) polymerase (PDB: 7kk4). Pharmacokinetic and toxicity analysis provided additional insights into the potential of these compounds as anti-cancer drugs. The computational analysis holds promise in accelerating the development of novel drug therapies aimed at treating various cancer types.
目标。
{"title":"A computational approach for screening of phytochemicals from Oxalis corniculata as promising anti-cancer candidates","authors":"","doi":"10.1016/j.jksus.2024.103383","DOIUrl":"10.1016/j.jksus.2024.103383","url":null,"abstract":"<div><p>Objectives.</p><p><em>In silico</em> techniques are a highly efficient, cost-effective, and rapid approach to identify potent herbal lead compounds with therapeutic potential against cancer. Phytochemicals are abundant in plants and may represent a promising cancer prevention and treatment approach. The plant species <em>Oxalis corniculata</em> has long been employed in traditional medicine to treat various diseases. Indeed, previous studies have demonstrated the anti-cancer properties of the crude extract obtained from this plant. However, further research is essential to elucidate precisely the underlying molecular mechanisms responsible for these activities.</p><p>Methods.</p><p>The study investigated the potential of bioactive compounds from <em>O. corniculata</em> to bind with different targets of anti-cancer drugs. Employing molecular docking and drug-likeness, 19 bioactive compounds from the plant were tested as potential anti-cancer leads. Compounds that exhibit both favorable drug-likeness and binding energies comparable to those of standard drugs like curcumin, doxorubicin, and paclitaxel were selected for further evaluation of their pharmacokinetic properties.</p><p>Results and Conclusions.</p><p>Among the 19 selected phytoconstituents of <em>O. corniculata</em>, compounds 15 and 16 exhibited the most effective binding energy (−8.68, −8.22 and −8.70, −8.22, −8.52 kcal/mol, respectively) with key cancer targets including Programmed death-ligand 1 (PDB: 7bea), B-Raf proto-oncogene, serine/threonine kinase (PDB: 8c7y) and poly (ADP-ribose) polymerase (PDB: 7kk4). Pharmacokinetic and toxicity analysis provided additional insights into the potential of these compounds as anti-cancer drugs. The computational analysis holds promise in accelerating the development of novel drug therapies aimed at treating various cancer types.</p></div>","PeriodicalId":16205,"journal":{"name":"Journal of King Saud University - Science","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1018364724002957/pdfft?md5=96506597c6db5f84725bb4b39ca10815&pid=1-s2.0-S1018364724002957-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141948707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-08-02DOI: 10.1016/j.jksus.2024.103382
Background
Barley (Hordeum vulgare L.) is the most cultivated cool-season cereal after wheat in Türkiye. This can be attributed to its uses in the malt industry, human consumption, and animal nutrition etc. This study investigated yield, physiological, and quality traits of different barley varieties using heatmap and principal component analysis (PCA) technique to identify the varieties with better yield and superior quality.
Methods
A total of 12 barley varieties (8 two-row and 4 six-row) were used in the study. The varieties were sown under natural conditions and data relating to yield, phenology and quality traits (grain yield, heading time, plant height, number of spikes m−2, 1000-grain weight, and starch ratio) were recorded. The recorded data were analyzed by PCA and heatmap techniques to determine the better-performing varieties.
Results
Significant variations were observed among the tested varieties for all evaluated traits (p < 0.01). Heatmap categorized the recorded traits into two clusters, and traits within the same cluster exhibiting interconnectedness. The primary cluster comprised of plant height, heading time, and 1000-grain weight. Similarly, the second significant cluster contained the varieties with similar grain yield, starch ratio, and the number of spikes m−2. The varieties ‘Bozlak’ and ‘Mert’, ‘Aydanhanim’ and ‘Tosunpaşa’, ‘Erciyes’ and ‘Çatalhüyük’, and ‘Sabribey’ and ‘Asutay’, exhibited significant resemblance for the recorded traits. The PCA revealed that 1000-grain weight was associated with the heading time, whereas starch ratio was associated with the grain yield. ‘Erciyes’ (3.58 tons ha−1), ‘Çatalhüyük 2001′ (2.95 tons ha−1), and ‘Bozlak’ (2.72 tons ha−1) recorded the highest yield, whereas ‘Erciyes’ variety resulted in the delayed heading. Similarly, ‘Çatalhüyük’, and ‘Erciyes’ produced the highest number of spikes m−2. Likewise, ‘Çatalhüyük 2001′, and ‘Tosunpaşa’ resulted in the heaviest 1000-grains, while ‘Asutay’ resulted in the highest starch ratio.
Conclusion
It is concluded that ‘Çatalhüyük’, ‘Erciyes’, and ‘Tosunpaşa’ are superior varieties in terms of yield-related traits, whereas ‘Asutay’ proved better for starch ratio. Therefore, these varieties could be used in future breeding programs to improve these traits.
{"title":"Interpretation of yield and quality traits of barley (Hordeum vulgare L.) varieties by heatmap technique","authors":"","doi":"10.1016/j.jksus.2024.103382","DOIUrl":"10.1016/j.jksus.2024.103382","url":null,"abstract":"<div><h3>Background</h3><p>Barley (<em>Hordeum vulgare</em> L.) is the most cultivated cool-season cereal after wheat in Türkiye. This can be attributed to its uses in the malt industry, human consumption, and animal nutrition etc. This study investigated yield, physiological, and quality traits of different barley varieties using heatmap and principal component analysis (PCA) technique to identify the varieties with better yield and superior quality.</p></div><div><h3>Methods</h3><p>A total of 12 barley varieties (8 two-row and 4 six-row) were used in the study. The varieties were sown under natural conditions and data relating to yield, phenology and quality traits (grain yield, heading time, plant height, number of spikes m<sup>−2</sup>, 1000-grain weight, and starch ratio) were recorded. The recorded data were analyzed by PCA and heatmap techniques to determine the better-performing varieties.</p></div><div><h3>Results</h3><p>Significant variations were observed among the tested varieties for all evaluated traits (p < 0.01). Heatmap categorized the recorded traits into two clusters, and traits within the same cluster exhibiting interconnectedness. The primary cluster comprised of plant height, heading time, and 1000-grain weight. Similarly, the second significant cluster contained the varieties with similar grain yield, starch ratio, and the number of spikes m<sup>−2</sup>. The varieties ‘Bozlak’ and ‘Mert’, ‘Aydanhanim’ and ‘Tosunpaşa’, ‘Erciyes’ and ‘Çatalhüyük’, and ‘Sabribey’ and ‘Asutay’, exhibited significant resemblance for the recorded traits. The PCA revealed that 1000-grain weight was associated with the heading time, whereas starch ratio was associated with the grain yield. ‘Erciyes’ (3.58 tons ha<sup>−1</sup>), ‘Çatalhüyük 2001′ (2.95 tons ha<sup>−1</sup>), and ‘Bozlak’ (2.72 tons ha<sup>−1</sup>) recorded the highest yield, whereas ‘Erciyes’ variety resulted in the delayed heading. Similarly, ‘Çatalhüyük’, and ‘Erciyes’ produced the highest number of spikes m<sup>−2</sup>. Likewise, ‘Çatalhüyük 2001′, and ‘Tosunpaşa’ resulted in the heaviest 1000-grains, while ‘Asutay’ resulted in the highest starch ratio.</p></div><div><h3>Conclusion</h3><p>It is concluded that ‘Çatalhüyük’, ‘Erciyes’, and ‘Tosunpaşa’ are superior varieties in terms of yield-related traits, whereas ‘Asutay’ proved better for starch ratio. Therefore, these varieties could be used in future breeding programs to improve these traits.</p></div>","PeriodicalId":16205,"journal":{"name":"Journal of King Saud University - Science","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1018364724002945/pdfft?md5=daefecaa1864f33f89226b0baa31fb36&pid=1-s2.0-S1018364724002945-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141948824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-08-02DOI: 10.1016/j.jksus.2024.103380
The biogas production from microalgae has gained attention due to fast depleting of fossil fuels and oil reserves. This study evaluated the anaerobic co-digestion of microalgae in various concentrations with cow manure to enhance biogas production. The biogas production of each experiment was measured using the water displacement method. The results indicated that the addition of microalgae significantly enhanced biogas production. Particularly, high methane yield of Anabaena sp. 50 %, Chlorella sp. 50 %, control was 345 ± 2.88 mL CH4/g VS, 297.96 ± 0.49 mL CH4/g VS, 138.32 ± 0.50 CH4/g VS respectively. The slurry produced by 50 % Anabaena sp. biogas plant exhibited the greatest level of seed germination. The current study demonstrated that Sorgham bicolor had the highest seed germination rate (94.2 %) root and shoot length of all crops. Therefore, it is possible to employ Anabaena sp. (50 %) and Chlorella sp. (50 %) in the rapid production of biogas. Moreover, agricultural output would be increased by using biogas slurry.
{"title":"Anaerobic co-digestion of cow manure and microalgae to increase biogas production: A sustainable bioenergy source","authors":"","doi":"10.1016/j.jksus.2024.103380","DOIUrl":"10.1016/j.jksus.2024.103380","url":null,"abstract":"<div><p>The biogas production from microalgae has gained attention due to fast depleting of fossil fuels and oil reserves. This study evaluated the anaerobic co-digestion of microalgae in various concentrations with cow manure to enhance biogas production. The biogas production of each experiment was measured using the water displacement method. The results indicated that the addition of microalgae significantly enhanced biogas production. Particularly, high methane yield of <em>Anabaena</em> sp. 50 %, <em>Chlorella</em> sp. 50 %, control was 345 ± 2.88 mL CH4/g VS, 297.96 ± 0.49 mL CH4/g VS, 138.32 ± 0.50 CH4/g VS respectively. The slurry produced by 50 % <em>Anabaena</em> sp. biogas plant exhibited the greatest level of seed germination. The current study demonstrated that Sorgham bicolor had the highest seed germination rate (94.2 %) root and shoot length of all crops. Therefore, it is possible to employ <em>Anabaena</em> sp. (50 %) and Chlorella sp. (50 %) in the rapid production of biogas. Moreover, agricultural output would be increased by using biogas slurry.</p></div>","PeriodicalId":16205,"journal":{"name":"Journal of King Saud University - Science","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1018364724002921/pdfft?md5=464e751b532a7e7f6202b8d548834765&pid=1-s2.0-S1018364724002921-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141962365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-31DOI: 10.1016/j.jksus.2024.103377
Hepatitis B virus (HBV) and its associated chronic liver disease pose a significant health hazard. Chronic HBV infection is a major contributor to the development of liver cirrhosis, liver fibrosis, and liver cancer. This study focused on ISG12a and its inhibitory effect in Huh7 cells on HBV gene expression and replication. The mammalian hepatoma cells Huh7 were transfected with the pHBV1.3 vector (pCAGGS) or HA-tagged ISG12a to determine the overexpression, to test this hypothesis further, we did knockdown or silencing by transducing Huh7 cells with lentiviruses containing shRNAs targeting ISG12a or scramble control shRNA (shControl). The expression of ISG12a was evaluated through western blot analysis. The HBsAg and HBeAg secretion in the Huh7 cells’ culture media was examined using an ELISA test. The qRT-PCR confirmed the mRNA and 3.5 mRNA Kb of ISG12a. The HBV total RNA was extracted and evaluated through a Northern blot. The isolated DNA was detected through qPCR. Additionally, a mechanistic study of enhancer II (EnhII/Cp) was examined through luciferase reporter testing. Our research findings indicate that ISG12a, an interferon-stimulated gene (ISG), plays a crucial role in suppressing the replication and gene expression of HBV. According to our study using the Huh7 cell system, overexpression of ISG12a resulted in a notable reduction in HBV protein levels as well as intracellular core-associated DNA and RNA levels. On the other hand, silencing ISG12a in Huh7 cells resulted in increased HBV RNA transcripts, DNA, and secreted proteins, indicating that ISG12a plays a role in suppressing HBV replication. Additionally, the research revealed that ISG12a inhibits the function of the EnhII/Cp promoter, which results in reduced HBV gene expression. The EnhII/Cp promoter is involved in regulating HBV gene expression, and the study showed that ISG12a restricts its activity. ISG12a may have a regulatory role in controlling the expression of HBV genes. These findings highlight the importance of ISG12a in HBV gene expression and provide valuable insights for understanding its antiviral function.
{"title":"Interferon-stimulated gene (ISG12a) suppresses hepatitis B virus replication in Huh 7 cells line","authors":"","doi":"10.1016/j.jksus.2024.103377","DOIUrl":"10.1016/j.jksus.2024.103377","url":null,"abstract":"<div><p>Hepatitis B virus (HBV) and its associated chronic liver disease pose a significant health hazard. Chronic HBV infection is a major contributor to the development of liver cirrhosis, liver fibrosis, and liver cancer. This study focused on ISG12a and its inhibitory effect in Huh7 cells on HBV gene expression and replication. The mammalian hepatoma cells Huh7 were transfected with the pHBV1.3 vector (pCAGGS) or HA-tagged ISG12a to determine the overexpression, to test this hypothesis further, we did knockdown or silencing by transducing Huh7 cells with lentiviruses containing shRNAs targeting ISG12a or scramble control shRNA (shControl). The expression of ISG12a was evaluated through western blot analysis. The HBsAg and HBeAg secretion in the Huh7 cells’ culture media was examined using an ELISA test. The qRT-PCR confirmed the mRNA and 3.5 mRNA Kb of ISG12a. The HBV total RNA was extracted and evaluated through a Northern blot. The isolated DNA was detected through qPCR. Additionally, a mechanistic study of enhancer II (EnhII/Cp) was examined through luciferase reporter testing. Our research findings indicate that ISG12a, an interferon-stimulated gene (ISG), plays a crucial role in suppressing the replication and gene expression of HBV. According to our study using the Huh7 cell system, overexpression of ISG12a resulted in a notable reduction in HBV protein levels as well as intracellular core-associated DNA and RNA levels. On the other hand, silencing ISG12a in Huh7 cells resulted in increased HBV RNA transcripts, DNA, and secreted proteins, indicating that ISG12a plays a role in suppressing HBV replication. Additionally, the research revealed that ISG12a inhibits the function of the EnhII/Cp promoter, which results in reduced HBV gene expression. The EnhII/Cp promoter is involved in regulating HBV gene expression, and the study showed that ISG12a restricts its activity. ISG12a may have a regulatory role in controlling the expression of HBV genes. These findings highlight the importance of ISG12a in HBV gene expression and provide valuable insights for understanding its antiviral function.</p></div>","PeriodicalId":16205,"journal":{"name":"Journal of King Saud University - Science","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1018364724002891/pdfft?md5=49dc7039f29637f073a0d40dc73c82cc&pid=1-s2.0-S1018364724002891-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141948825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-31DOI: 10.1016/j.jksus.2024.103378
Trichoderma species are widely acknowledged as growth-promoting fungi that have been utilized to enhance the growth and yield of numerous crops. This research examined the capacity of 30 Trichoderma strains, isolated from two organic rice fields in West Java, Indonesia, to enhance the germination, growth, and physiological characteristics of rice plants (Oryza sativa L.). In general, Trichoderma strain TM10 demonstrated the greatest ability to increase seed germination (97.25 %), vigor index (3122.83), and germination speed (59.91 seeds/day). This strain also increased seedling root length and seedling height by 101.62 % and 112.20 %, respectively. Plants treated with TM10 exhibited a notable improvement in root length, plant height, fresh weight, and dry weight compared to control plants, demonstrating increases of 188.68 %, 69.90 %, 157.41 %, and 159.38 %, respectively. Furthermore, the total chlorophyll content and stomatal number in TM10-treated plants exhibited increments of 75.23 % and 75.53 %, respectively. Five selected isolates (TM7, TM10, SB2, SB8, and SB14) were evaluated for their potential to produce plant growth-promoting compounds, including phosphatase enzyme (ranging from 0.54 to 11.14 µg pNP g−1h−1), indole-3-acetic acid (IAA) (ranging from 28.96 to 63.91 µg/mL), ammonia (ranging from 1.96 to 5.79 µg/mL), and hydrogen cyanide (HCN) (ranging from 221.76 to 274.82 ppm). The best strain, namely TM10, was then molecularly identified as Trichoderma yunnanense. This investigation demonstrates that Trichoderma spp. isolated from organic rice fields could be used as a bioinoculant in sustainable rice production.
{"title":"Isolation, screening, and molecular characterization of indigenous Trichoderma isolates from West Java, Indonesia and their plant growth-promoting capability on rice plants (Oryza sativa L.)","authors":"","doi":"10.1016/j.jksus.2024.103378","DOIUrl":"10.1016/j.jksus.2024.103378","url":null,"abstract":"<div><p><em>Trichoderma</em> species are widely acknowledged as growth-promoting fungi that have been utilized to enhance the growth and yield of numerous crops. This research examined the capacity of 30 <em>Trichoderma</em> strains, isolated from two organic rice fields in West Java, Indonesia, to enhance the germination, growth, and physiological characteristics of rice plants (<em>Oryza sativa</em> L.). In general, <em>Trichoderma</em> strain TM10 demonstrated the greatest ability to increase seed germination (97.25 %), vigor index (3122.83), and germination speed (59.91 seeds/day). This strain also increased seedling root length and seedling height by 101.62 % and 112.20 %, respectively. Plants treated with TM10 exhibited a notable improvement in root length, plant height, fresh weight, and dry weight compared to control plants, demonstrating increases of 188.68 %, 69.90 %, 157.41 %, and 159.38 %, respectively. Furthermore, the total chlorophyll content and stomatal number in TM10-treated plants exhibited increments of 75.23 % and 75.53 %, respectively. Five selected isolates (TM7, TM10, SB2, SB8, and SB14) were evaluated for their potential to produce plant growth-promoting compounds, including phosphatase enzyme (ranging from 0.54 to 11.14 µg pNP g<sup>−1</sup>h<sup>−1</sup>), indole-3-acetic acid (IAA) (ranging from 28.96 to 63.91 µg/mL), ammonia (ranging from 1.96 to 5.79 µg/mL), and hydrogen cyanide (HCN) (ranging from 221.76 to 274.82 ppm). The best strain, namely TM10, was then molecularly identified as <em>Trichoderma yunnanense</em>. This investigation demonstrates that <em>Trichoderma</em> spp. isolated from organic rice fields could be used as a bioinoculant in sustainable rice production.</p></div>","PeriodicalId":16205,"journal":{"name":"Journal of King Saud University - Science","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1018364724002908/pdfft?md5=73c19faa4b98985ad21677adf07eaaf4&pid=1-s2.0-S1018364724002908-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141948826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-30DOI: 10.1016/j.jksus.2024.103354
This work aims to motivate the bipolar parametric metric space introduced by Pasha et al. We introduce the concept of bipolar parametric -metric space. Afterward, we state and investigate new fixed-point theorems. Suitable examples are given based on our outcomes. An application is provided to strengthen the findings we obtained.
{"title":"Efficient techniques on bipolar parametric ν-metric space with application","authors":"","doi":"10.1016/j.jksus.2024.103354","DOIUrl":"10.1016/j.jksus.2024.103354","url":null,"abstract":"<div><p>This work aims to motivate the bipolar parametric metric space introduced by Pasha et al. We introduce the concept of bipolar parametric <span><math><mi>ν</mi></math></span>-metric space. Afterward, we state and investigate new fixed-point theorems. Suitable examples are given based on our outcomes. An application is provided to strengthen the findings we obtained.</p></div>","PeriodicalId":16205,"journal":{"name":"Journal of King Saud University - Science","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1018364724002660/pdfft?md5=7b2df1f2d2c49d7b11b3974443247d0e&pid=1-s2.0-S1018364724002660-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141948828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-29DOI: 10.1016/j.jksus.2024.103373
Herein, we report the synthesis of ZnO nanoparticles (ZnO-CB NPs) by employing the solution combustion method using an aqueous extract of brinjal calyxes as fuel. Characterization techniques, such as X-ray diffraction (XRD), Fourier transform Infrared spectroscopy (FTIR), UV–visible spectroscopy, and Scanning electron microscopy (SEM), were used to investigate the structural, optical, and morphological properties of synthesized nanoparticles, respectively. Highly porous hexagonal crystalline ZnO-CB NPs with less than 7 nm particle size were obtained. The photocatalytic performance of synthesized material is measured with Malachite green (MG), Basic brown 1 (BB1), and Acid orange 36 (AO36) as benchmark dyes. It showed that the synthesized material worked effectively under pH 10 with UV light irradiation. The synthesized ZnO-CB NP shows good removal effectiveness of the MG, BB1, and AO36 dyes with 99.3 %, 99.6 %, and 99.5 %, respectively, which can be promising photocatalysts for ecological applications such as wastewater remediation. Further, the synthesized ZnO-CB NP was used as blends in the methyl ester of Millettia pinnata oil (MPME), which is blended 20 % with commercial diesel (MPME20). The synthesized ZnO-CB NP was added to the MPME20 in varying amounts to ascertain its effects on the quality of emissions of various greenhouse gases such as hydrocarbons, COx, and NOx. Moreover, brake thermal efficiency (BTHE) and brake-specific fuel consumption (BSFC) were studied for the blends. The blend MPME20 with 25 mg of ZnO-CB NP, i.e., MPME20-25 mg, ZnO-CB, displays the best performance and reduced emissions.
{"title":"Photocatalytic and eco-emission applications of green synthesized ZnO-CB nanoparticles","authors":"","doi":"10.1016/j.jksus.2024.103373","DOIUrl":"10.1016/j.jksus.2024.103373","url":null,"abstract":"<div><p>Herein, we report the synthesis of ZnO nanoparticles (ZnO-CB NPs) by employing the solution combustion method using an aqueous extract of brinjal calyxes as fuel. Characterization techniques, such as X-ray diffraction (XRD), Fourier transform Infrared spectroscopy (FTIR), UV–visible spectroscopy, and Scanning electron microscopy (SEM), were used to investigate the structural, optical, and morphological properties of synthesized nanoparticles, respectively. Highly porous hexagonal crystalline ZnO-CB NPs with less than 7 nm particle size were obtained. The photocatalytic performance of synthesized material is measured with Malachite green (MG), Basic brown 1 (BB1), and Acid orange 36 (AO36) as benchmark dyes. It showed that the synthesized material worked effectively under pH 10 with UV light irradiation. The synthesized ZnO-CB NP shows good removal effectiveness of the MG, BB1, and AO36 dyes with 99.3 %, 99.6 %, and 99.5 %, respectively, which can be promising photocatalysts for ecological applications such as wastewater remediation. Further, the synthesized ZnO-CB NP was used as blends in the methyl ester of <em>Millettia pinnata</em> oil (MPME), which is blended 20 % with commercial diesel (MPME20). The synthesized ZnO-CB NP was added to the MPME20 in varying amounts to ascertain its effects on the quality of emissions of various greenhouse gases such as hydrocarbons, CO<sub>x</sub>, and NO<sub>x</sub>. Moreover, brake thermal efficiency (BTHE) and brake-specific fuel consumption (BSFC) were studied for the blends. The blend MPME20 with 25 mg of ZnO-CB NP, i.e., MPME20-25 mg, ZnO-CB, displays the best performance and reduced emissions.</p></div>","PeriodicalId":16205,"journal":{"name":"Journal of King Saud University - Science","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1018364724002854/pdfft?md5=527cd91abb78655845f30e35b2d46a77&pid=1-s2.0-S1018364724002854-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141961243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-29DOI: 10.1016/j.jksus.2024.103375
In the present study, potential probiotics were isolated from dairy products and characterized on a molecular basis. Male and female (16 + 16, n = 4) Wistar rats were randomly assigned to 5 groups to revise the effects of isolated potential probiotics on the gut immune system: 0-day, negative control, positive control (commercial product Lactobacillus acidophilus-14), laboratory isolated strains Pro-3 (Weissella confusa MZ735961), and Pro-4 (Lactiplantibacillus plantarum MZ727611). Aspartate-aminotransferase, Interleukin-6, bilirubin, and Alanine-transaminase values in both genders were expected but AST and creatinine values had a minor increase in the female group compared to the male probiotic-treated group. Cellular blood count level of mean corpuscular hemoglobin in male groups showed no considerable differences (p = 0.95), while there were decreased differences (p = 0.00) in female groups. The level of mean corpuscular hemoglobin concentration (MCHC) showed distinct variations (p = 0.02) in male groups, while these values were insignificant changes (p > 0.05) among female groups. No damage to the liver, thymus, or colon was ascertained based on morphological findings. The colon wall thickness, external muscle, crypt depth, and mucosa were significantly (p = 0.03) improved in rats given potential probiotics. In conclusion, probiotic supplementation stimulates the immune system. It may protect the intestinal mucosa by strengthening the gut’s immune system and promoting intestinal efficiency (nutrient absorption and number of goblet cells). L. plantarum can therefore offer a reliable, affordable, and safe treatment for functional gastrointestinal illnesses.
{"title":"Effect of probiotics on hematology and histopathology of organs associated with immune system in male and female Wistar rats","authors":"","doi":"10.1016/j.jksus.2024.103375","DOIUrl":"10.1016/j.jksus.2024.103375","url":null,"abstract":"<div><p>In the present study, potential probiotics were isolated from dairy products and characterized on a molecular basis. Male and female (16 + 16, n = 4) Wistar rats were randomly assigned to 5 groups to revise the effects of isolated potential probiotics on the gut immune system: 0-day, negative control, positive control (commercial product <em>Lactobacillus acidophilus</em>-14), laboratory isolated strains Pro-3 (<em>Weissella confusa</em> MZ735961), and Pro-4 (<em>Lactiplantibacillus plantarum</em> MZ727611). Aspartate-aminotransferase, Interleukin-6, bilirubin, and Alanine-transaminase values in both genders were expected but AST and creatinine values had a minor increase in the female group compared to the male probiotic-treated group. Cellular blood count level of mean corpuscular hemoglobin in male groups showed no considerable differences (p = 0.95), while there were decreased differences (p = 0.00) in female groups. The level of mean corpuscular hemoglobin concentration (MCHC) showed distinct variations (p = 0.02) in male groups, while these values were insignificant changes (p > 0.05) among female groups. No damage to the liver, thymus, or colon was ascertained based on morphological findings. The colon wall thickness, external muscle, crypt depth, and mucosa were significantly (p = 0.03) improved in rats given potential probiotics. In conclusion, probiotic supplementation stimulates the immune system. It may protect the intestinal mucosa by strengthening the gut’s immune system and promoting intestinal efficiency (nutrient absorption and number of goblet cells). <em>L. plantarum</em> can therefore offer a reliable, affordable, and safe treatment for functional gastrointestinal illnesses.</p></div>","PeriodicalId":16205,"journal":{"name":"Journal of King Saud University - Science","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1018364724002878/pdfft?md5=5a744bb32258c9f71139b2b047185457&pid=1-s2.0-S1018364724002878-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141948860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}