Journal of invertebrate pathology最新文献_第10页

Exploring the environmental distribution of the oyster parasite Haplosporidium costale 牡蛎寄生单孢子虫（Haplosporidium costale）的环境分布研究。

IF 2.4 3区生物学 Q1 ZOOLOGY

Journal of invertebrate pathology

Pub Date : 2025-09-21 DOI: 10.1016/j.jip.2025.108462

Isabelle Arzul , Cyrielle Lecadet , Bruno Chollet , Delphine Serpin , Lydie Canier , Xavier de Montaudouin

The protozoan parasite Haplosporidium costale is known to occur in the USA where it has been associated with sharp seasonal mortality of the Eastern oyster Crassostrea virginica since the 1960′s. In 2019, the parasite was detected for the first time in the Pacific oyster Magallana gigas in France in the context of light mortality and was subsequently detected in archived material collected since 2008. This detection raised several questions regarding the ability of the parasite to survive in the ecosystem and the potential involvement of other species in its life cycle. To answer these questions, an integrated sampling approach was deployed seasonally in three oyster farming areas where the parasite was known to occur. Parasite presence was evaluated after checking the presence of PCR inhibitors and using a previously developed and validated Real Time PCR assay, optimized in this study to detect parasite DNA in various environmental compartments. Parasite DNA was almost only detected in M. gigas. Considering the high number of oysters with low infection intensity, a complementary experiment was undertaken to better characterize sub-clinical infections in oysters. The presence of the parasite was tested twice a week in water and sediment from aquaria hosting M. gigas from a known infected site. After one month, oysters were sacrificed and tested using PCR or histology for the presence of the parasite at the tissular level. Altogether, field and experimental results indicate that the parasite is consistently established in oyster tissues, particularly in gills, which may act as a year-round reservoir of infection. The detection of parasite DNA in nanoplankton and sediment suggests that H. costale is released from the oysters in between mortality events. The potential for other species than M. gigas, particularly periwinkles, to be involved in the parasite life cycle deserves to be further investigated.

原生动物寄生虫单孢子虫已知发生在美国，自20世纪60年代以来，它与东部牡蛎的急剧季节性死亡有关。2019年，在法国的太平洋牡蛎Magallana gigas中首次发现了这种寄生虫，这是在轻度死亡的背景下发现的，随后在2008年以来收集的档案材料中发现了这种寄生虫。这一发现提出了关于寄生虫在生态系统中生存的能力以及其他物种在其生命周期中的潜在参与的几个问题。为了回答这些问题，在三个已知存在寄生虫的牡蛎养殖区采用了季节性的综合采样方法。在检查了PCR抑制剂的存在，并使用先前开发和验证的Real Time PCR检测后，评估了寄生虫的存在，本研究优化了该方法，用于检测各种环境隔间中的寄生虫DNA。寄生虫DNA几乎只在M. gigas中检测到。考虑到牡蛎数量多，感染强度低，进行了一项补充实验，以更好地表征牡蛎的亚临床感染。该寄生虫的存在每周两次在来自已知感染地点的养殖池的水和沉积物中进行检测。一个月后，牡蛎被处死，用PCR或组织学检测组织水平上寄生虫的存在。总之，现场和实验结果表明，这种寄生虫一直存在于牡蛎组织中，特别是在鳃中，这可能是全年感染的储存库。纳米浮游生物和沉积物中寄生虫DNA的检测表明，在两次死亡事件之间，牡蛎释放出了海岸螺杆菌。除长尾螺外，其他物种，特别是长春花螺，参与寄生虫生命周期的可能性值得进一步研究。

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引用次数: 0

Ingestion of polystyrene microplastics by Bombyx mori larvae disrupts midgut epithelial barrier integrity and potentially promotes susceptibility to BmNPV infection 家蚕幼虫摄入聚苯乙烯微塑料会破坏中肠上皮屏障的完整性，并可能促进对BmNPV感染的易感性。

IF 2.4 3区生物学 Q1 ZOOLOGY

Journal of invertebrate pathology

Pub Date : 2025-09-20 DOI: 10.1016/j.jip.2025.108463

Wenjing Shao , Yani Kang , Wenbin Yu , Yifei Kong , Yiming Huo , Zihan He , Xiaolong Hu , Xing Zhang

Microplastics (MPs) are ubiquitous environmental contaminants with potential implications for host-virus interactions, which has attracted global interest because MPs have been recognized as emerging pollutants. Despite growing concern over MPs, their influence on viral infections, particularly in invertebrate models, remains poorly understood. In this study, we employed the silkworm, Bombyx mori, as a model organism to investigate the impact of microplastic (MP) exposure on viral replication and the gut microbiome. Real-time PCR and Western blotting results showed that exposure to MPs was accompanied by significantly increased BmNPV viral gene expression (ie-1, vp39, gp41) and VP39 production in the midgut, suggesting an increase in BmNPV replication. Exposure to MPs disrupted gut microbiome composition, leading to a decrease in beneficial bacteria (e.g., Vibrionaceae) and an increase in opportunistic pathogens, potentially contributing to increased susceptibility to BmNPV. Notably, the increased permeability of the midgut epithelium in response to MP exposure likely facilitated viral entry and enhanced infection. These findings underscore the potential risks of MPs in facilitating viral replication and systemic infection and emphasize the necessity of further research to explore the complex relationships between MPs, gut microbiota, and viral infections across different ecological settings.

微塑料（MPs）是普遍存在的环境污染物，具有潜在的宿主-病毒相互作用的影响，这引起了全球的兴趣，因为MPs已被认为是新兴的污染物。尽管人们越来越关注MPs，但它们对病毒感染的影响，特别是在无脊椎动物模型中，仍然知之甚少。本研究以家蚕（Bombyx mori）为模型生物，研究微塑料（MP）暴露对病毒复制和肠道微生物组的影响。Real-time PCR和Western blotting结果显示，暴露于MPs后，BmNPV病毒基因表达（ie-1、vp39、gp41）和中肠vp39的产生显著增加，表明BmNPV复制增加。暴露于MPs会破坏肠道微生物组组成，导致有益细菌（如弧菌科）减少和机会致病菌增加，这可能会增加对BmNPV的易感性。值得注意的是，MP暴露后中肠上皮通透性的增加可能促进了病毒的进入和感染的增强。这些发现强调了MPs在促进病毒复制和全身性感染方面的潜在风险，并强调了进一步研究MPs、肠道微生物群和不同生态环境下病毒感染之间复杂关系的必要性。

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引用次数: 0

When a mosquito ingests blood, the phagocytic activity of hemocytes increases with warmer environmental temperature and aging 当蚊子摄取血液时，血细胞的吞噬活性随着环境温度的升高和老化而增加

IF 2.4 3区生物学 Q1 ZOOLOGY

Journal of invertebrate pathology

Pub Date : 2025-09-20 DOI: 10.1016/j.jip.2025.108459

Jordyn S. Barr, Saksham R. Saksena, Abby Callahan-Muller, Edith Simpson, Julián F. Hillyer

Most female mosquitoes feed on blood to gain the nutrients needed for oogenesis, but the process of blood feeding often exposes mosquitoes to pathogens. Blood feeding and infection both activate the hemocytes that drive cellular immune responses, and the efficacy of immune responses like phagocytosis varies with environmental temperature, aging, and their interaction. Here, we quantified the hemocyte-mediated phagocytosis response in the hemocoel of adult female mosquitoes during the digestion of a blood meal and thereafter, focusing on mosquitoes that had been reared at 27 °C, 30 °C or 32 °C, and were 3, 5 or 10 days old at the time of blood feeding. We discovered that, in blood-fed mosquitoes, phagocytic activity generally increases when the temperature is warmer and when the mosquito is older. Moreover, within the first three days after a blood meal, warmer temperature does not accelerate the senescence of the phagocytosis response. Altogether, these findings demonstrate that ingesting blood changes how warmer temperature, aging, and their interaction alters the phagocytic response of hemocytes, which has implications for how mosquitoes respond to infection and survive in their environment.

大多数雌蚊以血液为食，以获取产卵所需的营养，但吸血的过程经常使蚊子暴露于病原体。血液摄食和感染都激活了驱动细胞免疫反应的血细胞，而吞噬等免疫反应的效果随环境温度、衰老及其相互作用而变化。在此，我们量化了成年雌蚊在消化血粉期间和之后的血液中血细胞介导的吞噬反应，重点是在27°C， 30°C或32°C饲养的蚊子，吸血时为3,5和10日龄。我们发现，在血液喂养的蚊子中，当温度变暖和蚊子变老时，吞噬活动通常会增加。此外，在血餐后的前三天内，较高的温度并不会加速吞噬反应的衰老。总之，这些发现表明，摄入血液改变了温度升高、衰老以及它们之间的相互作用如何改变血细胞的吞噬反应，这对蚊子如何应对感染并在其环境中生存具有影响。

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引用次数: 0

Prevalence, replication, and tissue-specific localization of Euschistus heros virus (EhV) across life stages of the Neotropical brown stinkbug Euschistus heros (Hemiptera: Pentatomidae) 新热带褐臭虫Euschistus heros病毒（EhV）在整个生命阶段的流行、复制和组织特异性定位。

IF 2.4 3区生物学 Q1 ZOOLOGY

Journal of invertebrate pathology

Pub Date : 2025-09-17 DOI: 10.1016/j.jip.2025.108457

Ethiane Rozo Santos , Raul Alberto Laumann , Bergmann Morais Ribeiro , Andreza F. de Bem , Daniel M.P. Ardisson-Araújo

The Neotropical brown stink bug, Euschistus heros (Hemiptera: Pentatomidae), is a major agricultural pest in Brazilian soybean crops, exhibiting increasing insecticide resistance in field. This study investigates the dynamics of Euschistus heros virus (EhV), a multispecies iflavirus, in laboratory populations of E. heros, revealing a persistent, systemic virus infection across all life stages. Screening of 99 adults showed 100 % EhV genome prevalence in mated individuals, while virgin males and females had an 80 % infection rate. Viral loads peaked in freshly laid eggs (590,462 ± 27,426 copies/ng RNA), suggesting likely maternal transmission, before declining sharply in nymphs with an 18.08-fold reduction by the fifth instar. Adults exhibited sex- and mating-status-dependent variation, with virgin females harboring the highest copy number (227,380 ± 144,938 copies/ng RNA), which is 2.87-fold higher than mated females. Tissue-specific analysis revealed elevated EhV genome accumulation in legs/wings (221,723 ± 102,120 copies/ng RNA in males) and heads, with significantly lower loads in thoraces and abdomens. Strand-specific RT-qPCR suggested that EhV may exhibit replicative activity across developmental stages, as indicated by approximately balanced levels of genomic and replicative strands. Additionally, transmission electron microscopy of antennal tissues from an infected male individual revealed cytoplasmic icosahedral virions (∼30 nm), providing the first ultrastructural evidence of EhV assembly. These findings suggest that EhV may establish a stable, replicative association with E. heros and point to vertical transmission as a plausible mechanism of persistence. Nonetheless, confirmation of this pattern will require additional analyses with larger sample sizes, field collected insects, and complementary experimental approaches.

新热带褐臭虫（Euschistus heros，半翅目：蝽科）是巴西大豆田的主要农业害虫，在田间表现出越来越强的抗药性。本研究调查了一种多种黄疸病毒（EhV）在实验室种群中的动态。英雄们，在生命的各个阶段都有持续的全身性感染。99个成年个体的筛查显示，交配个体的EhV基因组患病率为100% %，而处女雄性和雌性的感染率为80% %。病毒载量在新产的卵中达到峰值（590,462 ± 27,426拷贝/ng RNA），表明可能是母体传播，然后在若虫中急剧下降，到第5龄时减少了18.08倍。成虫表现出性别和交配状态相关的变异，其中处女的RNA拷贝数最高（227,380 ± 144,938拷贝/ng RNA），是已交配雌虫的2.87倍。组织特异性分析显示，EhV基因组在腿/翅膀（雄性为221,723 ± 102,120拷贝/ng RNA）和头部的积累量升高，而在胸部和腹部的负荷显著降低。链特异性RT-qPCR表明，EhV可能在发育阶段表现出复制活性，这表明基因组和复制链的水平大致平衡。此外，来自受感染男性个体的触角组织的透射电子显微镜显示细胞质二十面体病毒粒子（~ 30 nm），提供了EhV组装的第一个超微结构证据。这些发现表明，超高压病毒可能与e.s heros病毒建立了稳定的、可复制的联系，并指出垂直传播是一种可能的持久性机制。然而，要确认这一模式，还需要对更大的样本量、实地采集的昆虫和补充性的实验方法进行进一步分析。

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引用次数: 0

Epidemiological investigation and whole genome of Vp AHPND CGVP22 isolated from an acute hepatopancreatic necrosis disease (AHPND) outbreak in Korea 韩国一起急性肝胰腺坏死病（AHPND）暴发中分离的Vp AHPND CGVP22的流行病学调查和全基因组分析

IF 2.4 3区生物学 Q1 ZOOLOGY

Journal of invertebrate pathology

Pub Date : 2025-09-16 DOI: 10.1016/j.jip.2025.108456

C.W.R. Gunasekara, L.G.T.G. Rajapaksha, S.H.M.P. Wimalasena, H.N.K.S. Pathirana, Gee-wook Shin

AHPN disease, caused by Vibrio parahaemolyticus, poses a significant threat to global shrimp farming. Here, we investigated the epidemiological relationships of the AHPND- causing Vp _AHPND CGVP22, isolated from the 2016 AHPND outbreak in Pyeongtaek, Korea, through whole-genome sequencing (WGS). Initially, the presence of pir^AB genes was confirmed by PCR and nested PCR assays, which produced specific bands of 284 base pairs (bp) for pir^A and 392 bp for pir^B, and a 230 bp fragment in the AP4 nested PCR. The assembled whole-genome sequence of Vp _AHPND CGVP22 comprised of a total size of approximately 5.4 Mbp, consisting of 2 circular chromosomes (3.4 Mbp and 2.0 Mbp) and a 69 kbp plasmid. The RAST genome annotation identified 386 subsystems, including those related to virulence, motility, and iron acquisition. In silico multi-locus sequence typing (MLST) assigned Vp _AHPND CGVP22 to sequence type ST1166, and clustered with other ST1166 strains from Vietnam and China. Comparative genomic analysis revealed that Vp _AHPND CGVP22 exhibited 52 single nucleotide polymorphisms (SNPs) and 99.96 % average nucleotide identity (ANI) relative to the 13028/A3 strain, and 973 SNPs with 99.87 % ANI compared to the 20130629002S01 strain. The plasmid of Vp _AHPND CGVP22 showed 99.94 % nucleotide identity to the pVPA3-1 plasmid of the 13028/A3 strain, suggesting a close phylogenetic relationship. Furthermore, Vp _AHPND CGVP22 and 13028/A3 shared identical prophages (12B12), antibiotic resistance genes, and virulence factors, while other ST1166 strains exhibit distinct profiles. Collectively, these results provide valuable insights into the genomic and evolutionary relationships of Vp _AHPND CGVP22, contributing to a better understanding of the epidemiology of AHPN disease.

由副溶血性弧菌引起的AHPN疾病对全球虾类养殖构成重大威胁。在这里，我们通过全基因组测序（WGS）研究了从韩国平泽市2016年AHPND暴发中分离的AHPND Vp CGVP22的流行病学关系。最初，通过PCR和巢式PCR检测证实了pirAB基因的存在，在AP4巢式PCR中产生了pirA的284个碱基对（bp）和pirB的392 bp的特异条带，以及230 bp的片段。组装的Vp AHPND CGVP22全基因组序列总大小约为5.4 Mbp，由2条圆形染色体（3.4 Mbp和2.0 Mbp）和一个69 kbp的质粒组成。RAST基因组注释鉴定出386个子系统，包括那些与毒力、运动性和铁获取相关的子系统。通过多位点序列分型（MLST）将Vp AHPND CGVP22定位于ST1166序列型，并与来自越南和中国的其他ST1166菌株聚类。对比基因组分析结果显示，Vp AHPND CGVP22与13028/A3相比，存在52个单核苷酸多态性（snp），平均核苷酸同源性（ANI）为99.96 %；与20130629002S01相比，存在973个snp，平均核苷酸同源性（ANI）为99.87 %。vpahpnd CGVP22与13028/A3株pVPA3-1质粒核苷酸同源性为99.94 %，系统发育关系密切。此外，Vp AHPND CGVP22和13028/A3具有相同的噬菌体（12B12）、抗生素抗性基因和毒力因子，而其他ST1166菌株具有不同的特征。总的来说，这些结果为Vp AHPND CGVP22的基因组和进化关系提供了有价值的见解，有助于更好地了解AHPN疾病的流行病学。

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引用次数: 0

Pathogen diversity of the non-native narrow-clawed crayfish (Pontastacus leptodactylus) in a UK water body 英国水体中非本地窄爪小龙虾的病原体多样性。

IF 2.4 3区生物学 Q1 ZOOLOGY

Journal of invertebrate pathology

Pub Date : 2025-09-16 DOI: 10.1016/j.jip.2025.108458

Matthew Harwood , Josie South , Alison M. Dunn , Paul D. Stebbing , Amy Burgess , Jamie Bojko

Biological invasions are intrinsically linked to introducing associated symbiotic organisms, some of which can be parasitic or pathogenic. The pathogenic risk of an ‘invasive parasite’ (aka. exotic pathogen) stems from its potential to infect native hosts and induce behavioural change or mortality, with the pathogen potentially presenting a greater risk than the host. Conversely, parasites translocated by invasive hosts may also reduce the impact of their host, indirectly curbing the hosts impact on the invaded ecosystem. In this study, we develop a pathogen profile for the narrow-clawed crayfish, Pontastacus leptodactylus. This is a non-native species in the United Kingdom, and poses a possible risk as a sink for invasive parasites. We use histopathology, metagenomics and metratranscriptomics to outline the symbiotic diversity harboured by a P. leptodactylus population from West Yorkshire, England.

We discovered several protozoan and bacterial species that appear to be putatively commensal with this invader, as well as several RNA viruses (Hepelivirales; Picornavirales; Nodaviridae, and others) that may be more pathogenic in nature. Microsporidia and Nudiviridae were absent in our population sample set, as were all metazoan obligate parasites, such as trematodes and acanthocephalans. Using the novel genomic and pathological data available to us, we have explored the evolutionary history of each symbiotic species and provided an initial assessment on the putative risk to native species.

生物入侵与引入相关的共生生物有着内在的联系，其中一些共生生物可能是寄生的或致病的。“侵入性寄生虫”的致病风险。外来病原体)源于其感染本地宿主并引起行为改变或死亡的潜力，病原体可能比宿主具有更大的风险。相反，被入侵宿主转移的寄生虫也可能减少其宿主的影响，间接抑制宿主对被入侵生态系统的影响。在这项研究中，我们建立了窄爪小龙虾，Pontastacus leptodactylus病原谱。这是英国的一种非本地物种，可能会成为入侵寄生虫的聚集地。我们使用组织病理学、宏基因组学和大转录组学来概述来自英国西约克郡的钩足绦虫种群的共生多样性。我们发现了几种原生动物和细菌物种似乎与这种入侵者共生，以及几种RNA病毒（Hepelivirales; Picornavirales； noddaviridae等），这些病毒在本质上可能更具致病性。我们的种群样本集中没有微孢子虫和裸病毒科，也没有所有的后生动物特异性寄生虫，如吸虫和棘头动物。利用新的基因组和病理学数据，我们探索了每个共生物种的进化史，并提供了对本地物种的假定风险的初步评估。

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引用次数: 0

Morphological and molecular characterization of a new species, Cyclopispora nantong n. gen. n. sp. from the adipose tissue of Eucyclops speratus (Crustacea: Cyclopidae) 一新种环棘虫南通n. Gen. n. Sp.的形态和分子特征。

IF 2.4 3区生物学 Q1 ZOOLOGY

Journal of invertebrate pathology

Pub Date : 2025-09-15 DOI: 10.1016/j.jip.2025.108454

Meiqi Weng , Chuangzheng Yang , Xunbo Miao , Nan Sun , Mei Cao , Suxin Liu , Jinyong Zhang , Xingqiang Wang

A new microsporidium, Cyclopispora nantong n. gen. n. sp., was described from the adipose tissue of Eucyclops speratus collected from a eutrophic water body of Nantong city, Jiangsu province, China. Infected copepods generally appeared opaque due to numerous spores accumulated in the adipose tissue. The earliest developmental stages observed were uninucleate meronts which resided in direct contact with the host cell cytoplasm. Mature spores were pyriform, monokaryotic, and measured 4.38 ± 0.20 (4.14–4.81) µm in length and 2.94 ± 0.09 (2.78–3.12) µm in width. The bipartite polaroplast consisted of the wide anterior lamellae and the tightly packed posterior lamellae. Isofilar polar filament coiled 13–16 turns and arranged in 3–4 rows. The 1306 bp SSU sequence obtained from E. speratus showed the highest similarity (85.9 %) to Hazardia milleri (AY09006) among the identified microsporidia. Phylogenetic analysis indicated that the present species clustered with an unidentified B. latissima lata-infecting species with high support values to form an independent branch which positioned the base of the Conglomerata-Gurleya-Agglomerata-Binucleata-Senoma-Pseudoberwaldia lineage. Based on the morphological characters and ultrastructural features, as well as SSU rDNA-inferred phylogenetic relationships, we propose the establishment of a novel genus (Cyclopispora n. gen.) and species (Cyclopispora nantong n. sp.) to contain this parasite.

摘要从江苏南通市富营养化水体中分离到一种新的小孢子虫Cyclopispore南通n. gen. n. sp.。由于大量孢子积聚在脂肪组织中，感染的桡足类动物通常表现为不透明。最早观察到的发育阶段是与宿主细胞质直接接触的无核小体。成熟孢子为梨形单核，长4.38 ± 0.20（4.14-4.81）µm，宽2.94 ± 0.09（2.78-3.12）µm。双侧极质体由宽的前片和紧密排列的后片组成。等丝极性灯丝卷成13-16圈，排列成3-4排。得到的1306 bp的SSU序列与所鉴定的细孢子虫AY09006的相似性最高（85.9 %）。系统发育分析表明，本种与一个未确定的具有高支持值的感染黑桫椤的种聚集在一起，形成了一个独立的分支，定位于glomerata- gurleya - agglomerata - binucleata - senoma - pseudoberwaldia谱系的基础。根据其形态特征和超微结构特征，以及SSU rdna推断的系统发育关系，我们提出了一个新的属（Cyclopispore n. gen.）和种（Cyclopispore n通n. sp.）来包含该寄生虫。

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引用次数: 0

American lobster (Homarus americanus) immune gene expression during Aerococcus viridans var. homari challenge 美国龙虾（Homarus americanus）在 绿色气球菌 var. homari 攻击中的免疫基因表达。

IF 2.4 3区生物学 Q1 ZOOLOGY

Journal of invertebrate pathology

Pub Date : 2025-09-15 DOI: 10.1016/j.jip.2025.108455

Zohreh Fazelan , Nicolas Argenta , Spencer J. Greenwood , K. Fraser Clark

The Canadian lobster (Homarus americanus) fishery is Canada’s most economically significant fishing industry and is a critical component of many rural Atlantic Canadian communities. Aerococcus viridans var. homari is a Gram-positive bacterium that can cause mortalities in wild and live-stocked lobsters. We used an RNA sequencing (RNA-seq) approach to examine the expression of more than 29,000 hepatopancreatic genes in H. americanus during an A. viridans var. homari infection challenge to determine how lobster immune gene expression changes during infection. The infection challenge identified 1,803 differentially expressed genes, of which at least 87 are related to immune or stress responses. Of particular note are several anti-lipopolysaccharide factors (ALFs), pentraxin serum amyloid A (SAA), lectins, Toll-like proteins, cytokines, relish, cactus, myeloid differentiation factor, and genes related to antioxidant defence, coagulation, and hyperglycemic hormone regulation. Compared to a previous study using microarray technology, our RNA-seq approach uncovered a broader array of immune genes that were previously undetected in H. americanus. This study provides additional evidence that ALFs and SAA-5-like proteins are critical players in immune defence. Differential expression of ALFs and lectin genes may indicate a tailored response specific to A. viridans var. homari, highlighting the complexity of the immune system of lobster in recognizing and responding to specific pathogens.

加拿大龙虾（Homarus americanus）渔业是加拿大最具经济意义的渔业，是许多大西洋加拿大农村社区的重要组成部分。同质绿气球菌是一种革兰氏阳性细菌，可导致野生和养殖龙虾死亡。我们使用RNA测序（RNA-seq）方法检测了在绿斑拟南蛙homari变异感染挑战期间美洲龙虾中超过29,000个肝胰腺基因的表达，以确定龙虾免疫基因表达在感染期间的变化。感染挑战鉴定了1803个差异表达基因，其中至少87个与免疫或应激反应有关。特别值得注意的是几种抗脂多糖因子（ALFs）、戊烷素血清淀粉样蛋白A （SAA）、凝集素、toll样蛋白、细胞因子、津津味、仙人掌、髓样分化因子以及与抗氧化防御、凝血和高血糖激素调节相关的基因。与先前使用微阵列技术的研究相比，我们的RNA-seq方法揭示了以前未在美洲人中检测到的更广泛的免疫基因序列。这项研究提供了额外的证据，证明ALFs和saa -5样蛋白在免疫防御中起关键作用。alf和凝集素基因的差异表达可能表明龙虾对特定病原体的特异性免疫反应，这凸显了龙虾免疫系统在识别和响应特定病原体方面的复杂性。

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引用次数: 0

Corrigendum to “Dissecting multitrophic interactions: The relationships among Entomophthora, their dipteran hosts, and associated bacteria” [J. Invertebr. Pathol. 213 (2025) 108425] 对“解剖多营养相互作用：昆虫疫霉及其双翅目宿主和相关细菌之间的关系”的更正[J]。Invertebr。中华病理学杂志。213 (2025)108425 [j]。

IF 2.4 3区生物学 Q1 ZOOLOGY

Journal of invertebrate pathology

Pub Date : 2025-09-11 DOI: 10.1016/j.jip.2025.108449

Zuzanna Płoszka , Karol H. Nowak , Marta Tischer , Anna Michalik , Michał R. Kolasa , Piotr Łukasik

引用次数: 0

Impact of helminth infection on sexual morphology, reproduction and survival of the freshwater amphipod, Hyalella patagonica 寄生虫感染对淡水片足类巴塔哥尼亚透明藻性形态、繁殖和存活的影响。

IF 2.4 3区生物学 Q1 ZOOLOGY

Journal of invertebrate pathology

Pub Date : 2025-09-09 DOI: 10.1016/j.jip.2025.108453

Carlos Rauque, Verónica Flores

Effects of helminth infection on sexual morphology and reproduction of the crustacean intermediate host are not well understood. Therefore, in the present study, we investigated the effects of parasitic infections on the amphipod Hyalella patagonica. Specimens were collected from Lake Mascardi, Argentina, between spring 2018 and summer 2019. We focused on three parasites: the acanthocephalans Pseudocorynosoma enrietti and Acanthocephalus tumescens and the nematode Hedruris suttonae. Morphological alterations in sexual traits, pairing success, and survival rates were analyzed. Significant morphological alterations were induced by P. enrietti, including castration and mixed sexual characteristics. Hedruris suttonae was the parasite most frequently found in amphipods in precopula, indicating that this parasite does not affect pairing success. Reduced survival rates were found in amphipods infected with H. suttonae or co-infected with P. enrietti and A. tumescens. These findings underscore the varying pathogenic effects of parasites on amphipod hosts and highlight the importance of considering multiple factors when evaluating host-parasite interactions and their ecological consequences in aquatic ecosystems.

蠕虫感染对甲壳类中间寄主性形态和繁殖的影响尚不清楚。因此，在本研究中，我们研究了寄生虫感染对片足类巴塔哥尼卡透明藻的影响。标本于2018年春季至2019年夏季期间从阿根廷马斯卡尔迪湖采集。我们重点研究了三种寄生虫：棘头虫、刺头虫、刺头虫和刺头线虫。分析了性性状、配对成功率和存活率的形态学变化。豚鼠的形态发生了明显的变化，包括去势和混合性征。在片脚类动物中最常见的寄生虫是suttonae，表明该寄生虫不影响配对成功。在片脚类动物中，感染了suttonae或同时感染了P. enrietti和A. tumescens的存活率降低。这些发现强调了寄生虫对片足类宿主的不同致病作用，并强调了在评估水生生态系统中宿主-寄生虫相互作用及其生态后果时考虑多种因素的重要性。

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