Journal of invertebrate pathology最新文献

{"title":"Protective effects of gallic acid against Vibrio parahaemolyticus-induced translucent post-larvae disease in Litopenaeus vannamei: insights into antibacterial and anti-virulence mechanisms","authors":"Man-Hong Ye ,&nbsp;Qian-Nan Han ,&nbsp;Chuang Meng ,&nbsp;Feng Ji ,&nbsp;Bin Zhou","doi":"10.1016/j.jip.2025.108499","DOIUrl":"10.1016/j.jip.2025.108499","url":null,"abstract":"<div><div>Translucent post-larvae disease (TPD) is a lethal syndrome causing high mortality in post-larvae of <em>Litopenaeus vannamei</em>. This study investigated the protective efficacy of gallic acid (GA), a non-antibiotic compound, against TPD induced by a field isolate <em>Vibrio parahaemolyticus</em> TS-GE (<em>V</em>. <em>para</em>. TS-GE). Immersion challenge assays confirmed the high virulence of <em>V</em>. <em>para</em>. TS-GE, as it caused 100 % mortality in post-larvae within 24 h at 2.82 × 10⁷ CFU/mL. Whole-genome sequencing revealed its genome comprised two chromosomes (3.50 Mb and 1.92 Mb) and three plasmids (69.7 kb, 60.7 kb, 60.5 kb). The 69.7-kb plasmid harbored TPD-associated virulence genes vhvp1 and vhvp2, while chromosomal genes encoded 40 type III secretion system components and thermolabile hemolysin. Pangenome analysis revealed the open genome nature of <em>V</em>. <em>parahaemolyticus</em> strains. Multi-locus sequence typing identified <em>V</em>. <em>para</em>. TS-GE as ST2621. <em>In vitro</em>, GA exhibited growth-inhibitory activity against <em>V</em>. <em>para</em>. TS-GE. <em>In vivo</em>, 200 μg/mL GA significantly reduced cumulative mortality (<em>P</em> &lt; 0.01) from 100 % to 18.3 %, preserving hepatopancreatic epithelium and midgut structure in <em>V</em>. <em>para</em>. TS-GE infected post-larvae. Mechanistic investigations revealed GA disrupted bacterial cell wall/membrane integrity, inhibited swimming motility, and suppressed biofilm formation. Molecular docking simulations predicted favorable binding of GA to virulence proteins VHVP1 (−6.3 kcal/mol) and VHVP2 (−7.8 kcal/mol), suggesting dual antibacterial and anti-virulence activities. These findings highlight GA as a promising antibiotic alternative for TPD control. Genomic data provide insights into the pathogenic adaptation of <em>V</em>. <em>para</em>. TS-GE in aquaculture.</div></div>","PeriodicalId":16296,"journal":{"name":"Journal of invertebrate pathology","volume":"214 ","pages":"Article 108499"},"PeriodicalIF":2.4,"publicationDate":"2025-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145573662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diet quality improves survival and mediates larval response to densovirus infection in the Melissa blue butterfly","authors":"Kelli J. McKeegan ,&nbsp;Matthew L. Forister ,&nbsp;Taylor Bradford ,&nbsp;Mike B. Teglas ,&nbsp;Angela M. Smilanich","doi":"10.1016/j.jip.2025.108497","DOIUrl":"10.1016/j.jip.2025.108497","url":null,"abstract":"<div><div>Entomopathogenic viruses affect insect populations by reducing fitness and influencing other measures of performance such as development and longevity. Given the huge diversity of viruses, studies are needed to understand the mechanisms underlying how viruses affect their insect hosts. The Junonia coenia densovirus (JcDV) infects a wide range of Lepidoptera and can cause death in the larval and pupal stages. In this study, we used the Melissa blue butterfly (<em>Lycaeides melissa</em>: Lycaenidae) to describe infection progression at the individual level, the effects of infection on development, survival, and longevity, and whether plant diet influenced these factors. Caterpillars were infected with JcDV and reared on either a low-quality host plant (<em>Medicago sativa</em>) or one of two high-quality host plants (<em>Astragalus canadensis</em> or <em>Lotus nevadensis</em>). We found that average viral load was similar across six days post-inoculation with a slight peak on day four. Viral load was highest among individuals reared on the low-quality host plant. Infection resulted in faster development time compared to uninfected individuals, and diet-specific effects were evident in survival to adulthood, with the native host plant (<em>A. canadensis</em>) offering optimal conditions for survival. None of the infected individuals that reached adulthood successfully eliminated JcDV, but viral loads were much lower in adults than larvae, indicating individuals reduced or maintained low viral loads between life stages. These results suggest diet-dependent response to JcDV infection and have implications for understanding the mechanisms that facilitate or impede the colonization of novel host plants.</div></div>","PeriodicalId":16296,"journal":{"name":"Journal of invertebrate pathology","volume":"214 ","pages":"Article 108497"},"PeriodicalIF":2.4,"publicationDate":"2025-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145549591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Substitutions in the cardiac glycoside binding site of the Na+/K+-ATPase do not explain differences in salinity tolerance between Steinernema entomopathogenic nematodes","authors":"Perla Achi ,&nbsp;Cullen McCarthy ,&nbsp;Lanie Bavier ,&nbsp;Robert Pena ,&nbsp;W. Nate Collison ,&nbsp;Victoria Iglesias ,&nbsp;Preston Christensen ,&nbsp;Ahmed Aljidui ,&nbsp;Anil Baniya ,&nbsp;Connor Goldy ,&nbsp;Rose C. Adrianza ,&nbsp;Suraj Reddy ,&nbsp;Simon C. Groen ,&nbsp;Adler R. Dillman","doi":"10.1016/j.jip.2025.108494","DOIUrl":"10.1016/j.jip.2025.108494","url":null,"abstract":"<div><div>Soil salinity varies widely across geographies both due to natural factors and human activities, including agriculture, road salt application, sea level rise, and desertification. Increases in soil salinity may affect organisms widely and particularly impact soil food webs. As parasites, entomopathogenic nematodes (EPNs) occupy crucial links in soil food webs and are important for agriculture as biological control agents of insect pests. Previous research found that the EPN <em>Steinernema carpocapsae</em> may exhibit higher salt tolerance than several of its congeners. We recently identified that <em>S. carpocapsae</em> uniquely evolved two amino acid substitutions in the first extracellular loop of the sodium pump (Na⁺/K⁺-ATPase). Here, we tested whether these substitutions explain <em>S. carpocapsae</em>’s reported lower sensitivity to salt. Our results confirm that <em>S. carpocapsae</em> exhibits higher salt tolerance and show that it can more effectively locate and infect insect hosts than its congeners <em>S. feltiae</em> and <em>S. hermaphroditum</em> in highly saline environments. We then retraced the evolution of the two amino acid substitutions in <em>S. carpocapsae</em> by introducing them alone and in combination in <em>Caenorhabditis elegans</em> using CRISPR genome engineering. We found that <em>C. elegans</em> mutants with single substitutions showed improved salt tolerance. However, this improvement disappeared in the double mutant, whose sodium pump mimicked that of <em>S. carpocapsae</em>. This pattern of negative epistasis between the amino acid substitutions suggests they are not responsible for variation in salt tolerance between <em>Steinernema</em> species. Sodium pump evolution in <em>S. carpocapsae</em> might instead be driven by encounters with cardiac glycosides, which are released into soil by several clades of plants including milkweeds, sequestered by some of this EPN’s herbivorous insect hosts, and known to target the first extracellular loop of the sodium pump. Our findings provide valuable insights into EPN adaptation to changes in environmental sodium levels and may have implications for their use in biological control.</div></div>","PeriodicalId":16296,"journal":{"name":"Journal of invertebrate pathology","volume":"214 ","pages":"Article 108494"},"PeriodicalIF":2.4,"publicationDate":"2025-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145534647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Density of Schistocerca cancellata locusts (Orthoptera) affects infection development of Paranosema locustae (Microsporidia)","authors":"Martina E. Pocco ,&nbsp;Alina Cerquetti ,&nbsp;Mariela Theiller ,&nbsp;Carlos E. Lange","doi":"10.1016/j.jip.2025.108496","DOIUrl":"10.1016/j.jip.2025.108496","url":null,"abstract":"<div><div>We investigated the occurrence of density-dependent prophylactic resistance (DDPR) in the South American locust, <em>Schistocerca cancellata</em>, by exposing gregarious-phase nymphs to the microsporidium <em>Paranosema locustae</em> across a range of densities. Infection prevalence and intensity were highest at low densities and declined sharply at intermediate densities. At high densities, infections were virtually absent. A threshold density for the expression of DDPR may exist, likely above 130 nymphs/m². These findings support the presence of DDPR in <em>S. cancellata</em> and suggest that <em>P. locustae</em> has limited potential as a biocontrol agent for managing gregarious populations during outbreaks.</div></div>","PeriodicalId":16296,"journal":{"name":"Journal of invertebrate pathology","volume":"214 ","pages":"Article 108496"},"PeriodicalIF":2.4,"publicationDate":"2025-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145530666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Research progress on the function of Toll pathway in heteropteran insects: A review","authors":"Wen-Hao Dong ,&nbsp;Huai-Jun Xue ,&nbsp;Yi-Peng Ren","doi":"10.1016/j.jip.2025.108495","DOIUrl":"10.1016/j.jip.2025.108495","url":null,"abstract":"<div><div>As an important component of innate immunity in insects, the Toll pathway is a conserved signaling cascade that activates humoral immunity through NF-κB-related pathways, contributing to the production of antimicrobial peptides (AMPs) and effectors in response to fungal, bacterial and viral infections. Although extensive advances have been made in the identification and function of the Toll pathway in insects, knowledge of the regulatory mechanisms of this pathway in heteropteran insects, for which the available knowledge is rare, is essential for exploring their developmental and immune functions to exploit species-specific and environmentally friendly bioinsecticides for control of heteropteran pests. In addition, increasing attention has been given to the activation, signal transduction and regulation of the Toll pathway in response to various stimuli in heteropteran insects because of the absence of some components of the immune deficiency (IMD) pathway and interaction with gut symbionts. In this review, we outline the current research progress on the functional roles of the Toll pathway in several heteropteran insects, such as <em>Riptortus pedestris</em>, <em>Rhodnius prolixus</em>, <em>Triatoma pallidipennis</em>, <em>Cimex lectularius</em>, <em>Plautia stali</em>, and others, which are involved in the responses to changes in feeding conditions, pathogenic infection, embryogenesis and wing polyphenism, as well as interactions with gut symbionts. Ultimately, we propose a framework for future research on the identification and functional characterization of the Toll pathway to expand the current data and address potential limitations on the investigation and application of this pathway in the design of pioneering pest control strategies for heteropteran pests.</div></div>","PeriodicalId":16296,"journal":{"name":"Journal of invertebrate pathology","volume":"214 ","pages":"Article 108495"},"PeriodicalIF":2.4,"publicationDate":"2025-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145516324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Confirmation of susceptibility of the mud crab Scylla paramamosain to Decapod iridescent virus 1 (DIV1)","authors":"Meng Wang ,&nbsp;Xiao-Ling Chen ,&nbsp;Xiao-Meng Guo ,&nbsp;Yu Wang ,&nbsp;Xin Guan ,&nbsp;Liang Qiu ,&nbsp;Jie Huang","doi":"10.1016/j.jip.2025.108491","DOIUrl":"10.1016/j.jip.2025.108491","url":null,"abstract":"<div><div>Decapod iridescent virus 1 (DIV1) is a newly identified pathogen responsible for significant disease outbreaks and high mortality rates in farmed crustaceans. While previous studies have reported the susceptibility of mud crab <em>Scylla paramamosain</em>, a species of considerable commercial importance in Asia, to DIV1, detailed investigations into its infection by DIV1, particularly in situ confirmation evidence, are still lacking. This study experimentally confirms that <em>S. paramamosain</em> is susceptible to DIV1, demonstrating that the virus can infect and induce disease through both intramuscular injection and oral routes. The infection resulted in high mortality, especially in the high-dose injection group, while oral exposure led to slower disease progression. Quantitative PCR analysis revealed high viral loads in hepatopancreas, gonads, and gills. Histopathological examination identified typical signs of infection, including eosinophilic inclusions and nuclear pyknosis. Furthermore, in situ DIG-labeling loop-mediated isothermal amplification (ISDL) provided direct evidence of the widespread distribution of DIV1 across various tissues, including the gonads, suggesting potential implications for reproduction. Transmission electron microscopy (TEM) revealed the presence of icosahedral viral particles with typical iridovirus morphology in infected cells, further confirming DIV1 replication in <em>S. paramamosain</em>. The study underscores the importance of the route of exposure in determining disease progression and mortality, with oral exposure resulting in lower mortality compared to injection. These findings establish <em>S. paramamosain</em> as a susceptible species for DIV1, expanding the known host range of the virus and offering valuable insights into its pathogenesis, tissue tropism, and potential impacts on aquaculture.</div></div>","PeriodicalId":16296,"journal":{"name":"Journal of invertebrate pathology","volume":"214 ","pages":"Article 108491"},"PeriodicalIF":2.4,"publicationDate":"2025-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145495888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intestinal health deficits in precocious Chinese mitten crab (Eriocheir sinensis): Integrated exploration of feeding behavior, histopathology, and microbiota","authors":"Jitao Zheng ,&nbsp;Yuxuan Zhao ,&nbsp;Qin Wu ,&nbsp;Yuchen Chen ,&nbsp;Yongchun Ge ,&nbsp;Xiaozhen Yang ,&nbsp;Yongxu Cheng","doi":"10.1016/j.jip.2025.108493","DOIUrl":"10.1016/j.jip.2025.108493","url":null,"abstract":"<div><div>Sexual precocity is a common problem in the aquaculture of Chinese mitten crabs (<em>Eriocheir sinensis</em>), and can reduce the aquaculture performance. The healthy intestines play a vital role in sustaining aquaculture performance. Therefore, this study investigated the effects of sexual precocity on the feeding behavior, histopathology, and intestinal microbiota of <em>E. sinensis</em>. Based on the data obtained, precocious crabs showed a longer feeding response time (136.51 ± 41.51 min) and a shorter feeding duration (36.46 ± 11.86 s) than non-precocious crabs (13.37 ± 3.96 min and 49.33 ± 21.95 s). Meanwhile, histological abnormalities were also observed in the intestines of precocious crabs. The histopathological results of the precocious crabs’ intestine revealed a larger intestinal diameter (1.113 ± 0.071 mm) and luminal area (0.621 ± 0.037 mm2), a thicker peritrophic membrane (13.870 ± 3.236 μm), as well as broken-down hindgut glands and disordered-arranged columnar epithelial cells. 16S rRNA gene sequencing analysis revealed deeper changes related to intestinal health in precocious crabs, with greater abundance of potentially pathogenic bacteria such as <em>Vibrio</em> and <em>Roseimarinus</em>. The potentially pathogenic bacteria in precocious crabs’ intestine such as <em>Mycoplasmataceae</em> and <em>Alphaproteobacteria</em> were found in greater abundance, while beneficial bacteria including <em>Rhizobiales</em> and <em>ZOR0006</em> were lower in abundance. Univariate correlation analysis revealed that <em>Rhizobiales</em> and <em>ZOR0006</em> had negative correlations with various potentially pathogenic bacteria including <em>Vibrio</em> and <em>Roseimarinus</em>. Based on the determination of microbiota in the water environment and SourceTrack analysis, the results indicated a decline in the proportion of intestinal microbiota derived from the water environment in precocious crabs. In conclusion, precocious crabs exhibit poorer intestinal health, which exacerbates aquaculture risks.</div></div>","PeriodicalId":16296,"journal":{"name":"Journal of invertebrate pathology","volume":"214 ","pages":"Article 108493"},"PeriodicalIF":2.4,"publicationDate":"2025-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145488780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of natural products and synthetic analogs which inhibit microsporidia spores and prevent infection","authors":"Qingyuan Huang ,&nbsp;Lauren E. Brown ,&nbsp;Guoqing Pan ,&nbsp;Junhong Wei ,&nbsp;John A. Porco Jr ,&nbsp;Jie Chen ,&nbsp;Aaron W. Reinke","doi":"10.1016/j.jip.2025.108492","DOIUrl":"10.1016/j.jip.2025.108492","url":null,"abstract":"<div><div>Microsporidiosis, a disease caused by microsporidia, affects many animals, with symptoms ranging from diarrhea to death, especially in immunocompromised individuals. Current treatments, such as the drugs albendazole and fumagillin, are limited in efficacy. To address this problem, we utilized <em>Caenorhabditis elegans</em> infected with its naturally occurring microsporidian parasite <em>Nematocida parisii</em> to evaluate 4,080 structurally diverse compounds from the Boston University Center for Molecular Discovery (BU-CMD) chemical library. From this screen, we identified 34 compounds that restored the reproductive capacity of <em>C. elegans</em>. We chose 17 compounds for additional validation experiments and all of them prevented <em>N. parisii</em> infection in <em>C. elegans</em>, with 10 of these capable of suppressing microsporidia invasion by inactivating mature spores. Additionally, five of the identified compounds were effective against <em>Pancytospora epiphaga</em>, a species related to human<em>-</em>infecting microsporidia. Together this work identifies and characterizes compounds that inhibit microsporidia infection.</div></div>","PeriodicalId":16296,"journal":{"name":"Journal of invertebrate pathology","volume":"214 ","pages":"Article 108492"},"PeriodicalIF":2.4,"publicationDate":"2025-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145476867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First report of Bonamia ostreae in wild flat oysters (Ostrea denselamellosa) from the west coast of Korea: Molecular and histopathological confirmation","authors":"Seung-Hyeon Kim ,&nbsp;Jinxia Long ,&nbsp;Tae-Sung Song ,&nbsp;Donghyun Lee ,&nbsp;Hyoun Joong Kim ,&nbsp;Hyung-Bae Jeon ,&nbsp;Yu Chen ,&nbsp;H.M.V. Udayantha ,&nbsp;Hye-Mi Lee ,&nbsp;Isabelle Arzul ,&nbsp;Henry S. Lane ,&nbsp;S.D.N.K. Bathige ,&nbsp;Kyung-Il Park","doi":"10.1016/j.jip.2025.108489","DOIUrl":"10.1016/j.jip.2025.108489","url":null,"abstract":"<div><div><em>Bonamia ostreae</em> is a protozoan parasite of significant concern in oyster aquaculture due to its impact on flat oyster species. In this study, we screened 30 wild flat oysters (<em>Ostrea denselamellosa</em>) collected from the western coastal region of South Korea for <em>B. ostreae</em> infection using two molecular assays. Species-specific BOSTRE-F/R primers targeting the ITS region detected 14/30 (46.67 %) positive samples, while <em>Bonamia</em> genus-specific Bo/Boas primers targeting the SSU rRNA region yielded 19/30 (63.33 %) positives. Although the higher detection rate with Bo/Boas primers raised the possibility of other <em>Bonamia</em> species, further PCR using <em>B. exitiosa</em>-specific primers confirmed the absence of <em>B. exitiosa</em>. To enhance species identification and phylogenetic analysis, extended SSU rDNA sequences (1,094 bp) were amplified using two primer sets, one newly developed for this study. Phylogenetic analysis demonstrated that the South Korean isolate clustered tightly within the <em>B. ostreae</em> clade alongside strains from Europe and North America, suggesting low genetic diversity and a possible recent introduction via human-mediated oyster movements. Histological examination and in situ hybridization (ISH) confirmed the presence of <em>B. ostreae</em> microcells localized within hemocytes and surrounding connective tissues. Despite successful detection, the infection intensity was low, and histopathological changes were mild, suggesting that <em>O. denselamellosa</em> may be relatively less susceptible to <em>B. ostreae</em>, though further investigation is needed to assess its role in the parasite’s transmission dynamics. These findings highlight the need for enhanced molecular surveillance and international biosecurity measures, as this study represents the first confirmed report of <em>B. ostreae</em> infection in <em>O. denselamellosa</em> and the first record of this parasite in East Asia.</div></div>","PeriodicalId":16296,"journal":{"name":"Journal of invertebrate pathology","volume":"214 ","pages":"Article 108489"},"PeriodicalIF":2.4,"publicationDate":"2025-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145451986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Daphniaspora nanhuensis n. gen. n. sp. infecting the intestine of Moina micrura (Crustacea: Moinidae) in China","authors":"Meiqi Weng ,&nbsp;Nan Sun ,&nbsp;Xunbo Miao ,&nbsp;Chenxi Zhang ,&nbsp;Mei Cao ,&nbsp;Suxing Liu ,&nbsp;Xingqiang Wang ,&nbsp;Jinyong Zhang","doi":"10.1016/j.jip.2025.108490","DOIUrl":"10.1016/j.jip.2025.108490","url":null,"abstract":"<div><div>We reported a new microsporidium, <em>Daphniaspora nanhuensis</em> n. gen. n. sp., from the intestine of <em>Moina micrura</em> collected from Lake Nanhu in Wuhan City, Hubei Province, China. Infected daphnids generally appeared to have hypertrophied and opaque intestines under light microscopy. Electron microscopy revealed all developmental stages residing in direct contact with the host cell cytoplasm. The earliest stages observed were uninucleate meronts with a round nucleus. Multinucleate sporogonial plasmodia developed into uninucleate sporoblasts by rosette-like budding. Mature spores were dumbbell-shaped, measuring 4.57 ± 0.40 (3.75–5.54) µm long and 1.79 ± 0.18 (1.40–2.23) wide. The bipartite polaroplast was composed of tightly packed anterior lamellae and wide posterior lamellae. Isofilar polar filaments coiled 5–6 turns and arranged in one row. The exospore consisted of three layers. BLASTn searches of obtained SSU rDNA sequence showed the highest similarity (78.52 %) with <em>Liebermannia dichroplusae</em> (GenBank accession number: EF016249). Phylogenetic analysis based on the obtained SSU rDNA sequence indicated that the present species represented an independent branch but formed a sister relationship with the genus <em>Liebermannia</em>. Based on the morphological characteristics, ultrastructural features, and molecular data, we propose the establishment of a novel genus (<em>Daphniaspora</em> n. gen.) and species (<em>Daphniaspora nanhuensis</em> n. sp.) to accommodate the present species.</div></div>","PeriodicalId":16296,"journal":{"name":"Journal of invertebrate pathology","volume":"214 ","pages":"Article 108490"},"PeriodicalIF":2.4,"publicationDate":"2025-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145458924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}