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Aneuploidy Rate, DNA Fragmentation, Tubulin and Centrin Content in Different Groups of Male Infertility 不同男性不育群体的非整倍体率、DNA片段化、微管蛋白和中心蛋白含量
Pub Date : 2020-09-01 DOI: 10.7176/jmpb/68-04
Ö. Budak, Furkan S Ceylan, Veysel Toprak, Ender Yalçınkaya, S. Kurnaz, M. Kasap, E. Çalışkan
In this study, we aimed to compare DNA fragmentation ratios, centrosomal protein concentrations (centrin and tubulin) and numerical chromosomal abnormalities between four groups including asthenozoospermia, teratozoospermia, oligoasthenozoospermia and normozoospermia patients, and to emphasize the necessity of investigating genetic and protein content of the spermatozoa also in patients with a semen analysis within normal reference range. Evaluation of semen analysis of the patients was performed based on WHO 2010 criteria. As a result of the statistical analysis, it was observed that the sperm DNA fragmentation rate in the normozoospermia group (6.50 ± 3.46%) was significantly lower than those in the teratozoospermia (19.69 ± 9.86%) and oligoasthenoteratozoospermia group (32.47± 14.13%) (p<0.001). For centrin and tubulin proteins, highest concentrations were observed in normozospermia group (p<0.05). When incidence of numerical abnormalities were compared between four groups, it was found that there were statistically significant differences between groups in terms of nullisomies X, Y and 13, and  21 (p<0.05). In conclusion, we suggest that abnormalities in semen parameters seem to be associated with chromosomal abnormalities; and sperm aneuploidy rate is also at a considerable level even in normozoospermic patients. Keywords: DNA fragmentation, teratozoospermia, centrin, tubulin DOI: 10.7176/JMPB/68-04 Publication date: September 30 th 2020
在本研究中,我们旨在比较弱精子症、畸形精子症、少弱精子症和正常精子症四组患者的DNA片段率、中心体蛋白浓度(中心体蛋白和微管蛋白)和染色体数量异常,并强调在正常参考范围内进行精液分析的患者中,也有必要调查精子的遗传和蛋白质含量。根据世卫组织2010年标准对患者精液分析进行评估。统计分析发现,正常精子症组精子DNA断裂率(6.50±3.46%)显著低于畸形精子症组(19.69±9.86%)和少弱畸形精子症组(32.47±14.13%),差异有统计学意义(p<0.001)。中心蛋白(centrin)和微管蛋白(tubulin)以无精子症组最高(p<0.05)。比较四组间数值异常发生率,X、Y、13、21的零组间差异有统计学意义(p<0.05)。总之,我们认为精液参数异常似乎与染色体异常有关;精子非整倍体率在正常精子患者中也处于相当高的水平。关键词:DNA片段化,畸形精子症,中心化,微管蛋白DOI: 10.7176/JMPB/68-04出版日期:2020年9月30日
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引用次数: 0
Our Experience in the Surgical Treatment of Chronic Constrictive Pericarditis 慢性缩窄性心包炎的外科治疗体会
Pub Date : 2020-09-01 DOI: 10.7176/jmpb/68-03
E. Likaj, S. Kuçi, S. Dumani, A. Refatllari
Introduction: We reviewed the cases of 21 consecutive patients who underwent surgery for chronic constrictive pericarditis between January 2004 and January 2016, to determine causes, surgical techniques, mortality and morbidity rates, and improvement of functional capacity. Patients and Methods: Eleven of our patients were men (52.3%) and ten were women (47.7%); their average age was 48.71±15.6 years. The pericarditis was tuberculous in 13 patients (61.9%), idiopathic in 4 patients (19%), rheumatic in two patients (9.5%), post surgical in one patient (4.75%) and neoplastic in one patient (4.75%).  Results: In all the cases, our approach was median sternotomy. All anterior pericardium was resected from left phrenic nerve to right phrenic nerve. No patient required cardiopulmonary bypass. We had no early postoperative mortality. In the 1st postoperative month, functional capacity improved dramatically: the number of patients in New York Heart Association functional class IV moved from 13 (preoperatively) to 4; in class III, from 7 to 3; in class II, from 1 to 6; and in class I, from 0 to 10.  Conclusions: We recommend the median sternotomy approach for chronic constrictive pericarditis. Tuberculosis still remains the main cause at our country. It can be treated with excellent results nowadays. Keywords: surgical treatment, chronic constrictive pericarditis DOI: 10.7176/JMPB/68-03 Publication date: September 30 th 2020
我们回顾了2004年1月至2016年1月期间连续21例接受手术治疗的慢性缩窄性心包炎患者的病例,以确定病因、手术技术、死亡率和发病率以及功能能力的改善。患者与方法:男性11例(52.3%),女性10例(47.7%);平均年龄48.71±15.6岁。心包炎结核性13例(61.9%),特发性4例(19%),风湿性2例(9.5%),术后1例(4.75%),肿瘤性1例(4.75%)。结果:所有病例均采用胸骨正中切开术。从左膈神经至右膈神经全部切除前心包。没有病人需要体外循环。我们没有术后早期死亡率。术后1个月,功能能力显著改善:纽约心脏协会功能等级IV的患者数量从术前的13例增加到4例;第三类,从7级到3级;第二类,1至6级;在第一类,从0到10。结论:我们推荐胸骨正中切口入路治疗慢性缩窄性心包炎。结核病仍然是我国的主要病因。现在治疗效果很好。关键词:手术治疗,慢性缩窄性心包炎DOI: 10.7176/JMPB/68-03出版日期:2020年9月30日
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引用次数: 0
Gastrointestinal Problems and Their Risk Factors Among Arba Minch Town Male Super League Football Club Players 阿尔巴明奇镇足球超级联赛男球员胃肠问题及其危险因素分析
Pub Date : 2020-08-05 DOI: 10.7176/jmpb/69-04
Belay Silesh, Molla Doyou
Objective: The objective of this study was to assess the prevalence of exercise induced gastrointestinal problems and their risk factors among Arba Minch town male super league football club players. Result: A total of 31subjects participated in the study with a response rate of 100 %. The prevalence of GI symptoms is found to be 45.2%. Taking of NSAIDs frequently, doing exercise frequently and intensively, performing exercise on hot and humid environment and taking laxatives frequently identified as high risk factors. Unlikely, intake of tea/coffee for a long time and taking hypertonic fluid frequently are less risky to develop GI problem. According to this study prevalence exercise-induced gastrointestinal problems is high. Therefore, the concerned bodies shall intervene to tackle the problem.
目的:本研究的目的是评估Arba Minch镇超级足球俱乐部男性球员运动性胃肠问题的患病率及其危险因素。结果:共有31名受试者参与研究,应答率为100%。胃肠道症状的患病率为45.2%。频繁服用非甾体抗炎药,频繁高强度运动,在湿热环境下进行运动,频繁服用泻药被认为是高危因素。不太可能,长期摄入茶/咖啡和经常服用高渗液体会降低患胃肠道疾病的风险。根据这项研究,运动引起的胃肠问题的患病率很高。因此,有关机构应介入解决这一问题。
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引用次数: 0
FREQUENCY OF ANEMIA AMONG WOMEN PRESENTING WITH SHORT INTER-PREGNANCY INTERVAL AT A TERTIARY CARE HOSPITAL. 三级保健医院妊娠间隔短的妇女贫血的频率。
Pub Date : 2020-08-01 DOI: 10.7176/jmpb/67-05
M. Masood, N. Nazar, M. A. Khan
Background; The time interval between pregnancies is considered to be an important and modifiable risk factor in terms of adverse perinatal outcomes. This study was done to  determine the frequency of anemia in pregnant women having short interpregnancy interval. Materials and methods; All the pregnant women with short interpregnancy interval were registered from Department of Gynecology and Obstetrics, Nishtar Hospital Multan, Pakistan. Examination was done including general physical examination. Those with interpregnancy interval less than 6 months were included in study. Detailed history was taken regarding parity, duration of pregnancy. Duration of gestation was estimated according to patients last menstrual period. Three ml venous blood sample was drawn and sent to the pathology laboratory of Nishtar Hospital Multan for Hb levels analysis. Data was entered and analyzed by SPSS version 20. Results; Mean age of our study cases was noted to be 27.83 ± 4.95 years (with minimum age of our study cases was 21 years while maximum age was noted to be 38 years). Of these 90 study cases, 51 (56.7%) were from rural areas, 73 (81.1%) were normal weight 11(12.2%) were overweight and 6 (6.7%) were obese. Seventy eight (86.7%) were illiterate. Of these 90 study cases, 73 (81.1%) were having poor socioeconomic status, 11 (12.2%) were diabetic and 17 (18.9%) were hypertensive. Mean body mass index (BMI) of our study cases was noted to be 22.31 ± 2.07 Kg/m2. Mean gestational age of our study cases was noted to be 24.02 ± 8.31 weeks. Mean Hb level was noted to 10.67 ± 0.893 g/dl (with minimum Hb level was 9.6 g/dl while maximum Hb level was 12.5 g/dl). Mean interpregnancy interval was 4.23 ± 0.98 months (with minimum interpregnancy interval was 2.5 months while maximum interpregnancy interval was 6 months). Anemia was noted in 74 (82.2%) our study cases and only 23 (25.6%) of our study cases were taking iron supplements. Conclusion; Short interpregnancy interval less than 6 months is related with adverse pregnancy outcomes. Very high frequency of anemia was noted in our study cases having interpregnancy interval less than 6 months. Anemia was significantly associated with residential status, hypertension and iron supplementation. Birth spacing is an issue which women should have some control over. Educational interventions, including birth control, should be applied during prenatal visits and following delivery. Keywords ; Short interpregnancy Interval, anemia, hemoglobin. DOI : 10.7176/JMPB/67-05 Publication date :August 31st 2020
背景;怀孕之间的时间间隔被认为是一个重要的和可改变的危险因素,就不利的围产期结局而言。本研究旨在确定妊娠间隔较短的孕妇贫血的发生频率。材料和方法;所有解释间隔较短的孕妇均登记于巴基斯坦木尔坦Nishtar医院妇产科。检查包括一般体格检查。翻译间隔小于6个月者纳入研究。详细记录了胎次、妊娠期。根据患者最后一次月经估计妊娠期。抽取静脉血3 ml送到木尔坦Nishtar医院病理实验室进行Hb水平分析。数据录入和分析采用SPSS version 20。结果;我们研究病例的平均年龄为27.83±4.95岁(我们研究病例的最小年龄为21岁,最大年龄为38岁)。90例病例中,农村51例(56.7%),体重正常73例(81.1%),超重11例(12.2%),肥胖6例(6.7%)。文盲78人(86.7%)。在90例研究病例中,73例(81.1%)社会经济状况较差,11例(12.2%)患有糖尿病,17例(18.9%)患有高血压。我们研究病例的平均体重指数(BMI)为22.31±2.07 Kg/m2。本研究病例的平均胎龄为24.02±8.31周。平均Hb水平为10.67±0.893 g/dl(最低Hb水平为9.6 g/dl,最高Hb水平为12.5 g/dl)。平均解释间隔为4.23±0.98个月(最小解释间隔2.5个月,最大解释间隔6个月)。74例(82.2%)的研究病例出现贫血,只有23例(25.6%)的研究病例服用了铁补充剂。结论;妊娠间隔短,小于6个月与不良妊娠结局有关。在我们的研究病例中,解释间隔小于6个月的贫血发生率很高。贫血与居住状态、高血压和补铁显著相关。生育间隔是一个女性应该有所控制的问题。应在产前检查和分娩后实施教育干预,包括节育。关键字;短解释间隔,贫血,血红蛋白。DOI: 10.7176/JMPB/67-05出版日期:2020年8月31日
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引用次数: 0
Assessment of Mothers Level of Knowledge About Neonatal Danger Signs and Its Associated Factors in St Paul’s Hospital Millenium Medical College Addis Ababa Ethiopia 埃塞俄比亚亚的斯亚贝巴圣保罗医院千禧医学院母亲对新生儿危险体征及其相关因素的知识水平评估
Pub Date : 2020-08-01 DOI: 10.7176/jmpb/67-02
M. Haile
Background: Neonatal danger has become a substantial problem in many developing countries like Ethiopia. More specifically, neonatal rates in Ethiopia are among the highest in the world. In this regard, health-seeking behavior of mothers for neonatal care highly relies on their knowledge about neonatal danger sign, and it has been hardly investigated. Therefore, this study was intended to determine the level of mother’s knowledge about neonatal danger signs and to identify factors associated with good mother’s knowledge. Objective: To assess mother’s level of knowledge about neonatal danger sign and its associated factors in St. Paul Hospital, Addis Ababa, Ethiopia. Methods: Institution based quantitative cross sectional study design was used to assess the knowledge and associated factors that influence neonatal danger signs knowledge among mothers attending postnatal. A total of 159 postnatal mothers was the calculated sample size and selected by simple random sampling technique. Data was collected by using a structured interview questionnaire through face to face interview by six data collectors. Data quality was assured through pretesting the questionnaire on 5% of postnatal mothers and findings from the pretest was used to amend the questionnaire. The data was entered and analyzed by SPSS version 23.0. Result: A total of 155 mothers were interviewed with the response rate of 97.5%. The mean age of mothers was 28.8 (SD ±5.4). More than one third (39.4%) of mothers were in the age range 25-30 years. The majority of mothers were married, 140 (90.3%); orthodox by religion, 84 (54.2%) and Oromo by ethnic group which was 75 (48.7%). Only 46 (29.7%) of all mothers had good knowledge which were having knowledge of at least four of the ten defined neonatal danger signs. Fever was more commonly known danger sign by 55.6% of the mothers. There were no any significant associations based on multivariate logistic regression. Conclusion and recommendations: Majority of mothers had poor knowledge of the majority of neonatal danger signs. Strengthening postnatal counselling at a health facility or dissemination of information community level about danger sign is highly recommended. DOI: 10.7176/JMPB/67-02 Publication date: August 31 st 2020
背景:在埃塞俄比亚等许多发展中国家,新生儿危险已成为一个重大问题。更具体地说,埃塞俄比亚的新生儿死亡率是世界上最高的。在这方面,母亲寻求新生儿保健的行为在很大程度上依赖于其对新生儿危险体征的了解,而这方面的调查很少。因此,本研究旨在确定母亲对新生儿危险体征的知识水平,并确定与好母亲知识相关的因素。目的:了解埃塞俄比亚亚的斯亚贝巴圣保罗医院产妇对新生儿危险体征及其相关因素的认知水平。方法:采用基于机构的定量横断面研究设计,评估产后产妇对新生儿危险体征知识的了解及其影响因素。计算样本量,采用简单随机抽样方法抽取159名产后母亲。数据收集采用结构化访谈问卷,由6位数据收集者进行面对面访谈。通过对5%的产后母亲进行问卷预测来保证数据质量,并使用预测的结果来修改问卷。采用SPSS 23.0版本进行数据录入和分析。结果:共访谈155名母亲,回复率为97.5%。母亲平均年龄28.8岁(SD±5.4)。超过三分之一(39.4%)的母亲年龄在25-30岁之间。大多数母亲已婚,140人(90.3%);宗教正统派84人(54.2%),民族奥罗莫派75人(48.7%)。所有母亲中只有46人(29.7%)有良好的知识,即至少了解10种确定的新生儿危险体征中的4种。55.6%的母亲认为发烧是更常见的危险信号。多变量logistic回归分析无显著相关性。结论和建议:大多数母亲对大多数新生儿危险体征的认识不足。强烈建议在保健机构加强产后咨询或在社区一级传播关于危险标志的信息。DOI: 10.7176/JMPB/67-02出版日期:2020年8月31日
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引用次数: 0
Investigation of Serum NO, ADMA and Apelin Levels in Thyroid Dysfunction 甲状腺功能障碍患者血清NO、ADMA、Apelin水平的研究
Pub Date : 2020-08-01 DOI: 10.7176/jmpb/67-03
A. Şahin, M. Gül, Ö. Akyol, E. Simsek, F. Çelebi, Akar Karakoç
Thyroid gland diseases are among the most common endocrine diseases and still continue to be an important health problem especially in developing countries. It was aimed to investigate serum NO, ADMA and Apelin levels in patients with thyroid dysfunction. This study was conducted with 150 thyroid patients and 50 healthy subjects. Study subjects were divided into three groups; control (n=50), hyperthyroid ( n=75) and hypothyroid (n=75). Serum TSH, FT3, FT4   levels  were  measured  by  chemiluminescence  method  NO  level  were  measured  by spectrophotometric method, ADMA and apelin levels were measured by ELISA. Serum NO levels were higher in hypothyroid group than in hyperthyroid group, and the difference was statistically significant. Serum ADMA levels of the hyperthyroid group were significantly higher than the other two groups and the difference was statistically significant. The levels  of  serum  apelin  were  statistically significantly higher in the hyperthyroid group than the other two groups. In patients with hyperthyroidism, ADMA and Apelin levels were higher, while NO level was lower. However, NO level was higher in patients with hypothyroidims than the other two groups. Apelin, which has been emphasized  as  a  preventive  and  therapeutic  agent  particularly  for  the cardiovascular system, might have increased in hyperthyroid patients, regardless of NO, to protect cardiovascular system from possible adverse effects of ADMA. Keywords: Asymmetric dimethylarginine, apelin, nitric oxide, thyroid dysfunction DOI: 10.7176/JMPB/67-03 Publication date: August 31 st 2020
甲状腺疾病是最常见的内分泌疾病之一,仍然是一个重要的健康问题,特别是在发展中国家。目的探讨甲状腺功能障碍患者血清NO、ADMA和Apelin水平。本研究对150名甲状腺患者和50名健康受试者进行了研究。研究对象被分为三组;对照组(n=50)、甲亢组(n=75)和甲亢组(n=75)。化学发光法测定血清TSH、FT3、FT4水平,分光光度法测定NO水平,酶联免疫吸附法测定ADMA和apelin水平。甲状腺功能减退组血清NO水平高于甲状腺功能亢进组,差异有统计学意义。甲亢组血清ADMA水平显著高于其他两组,差异有统计学意义。甲亢组血清apelin水平高于其他两组,差异有统计学意义。甲亢患者ADMA、Apelin水平较高,NO水平较低。甲减组一氧化氮水平明显高于其他两组。Apelin一直被强调为一种预防和治疗药物,特别是对心血管系统,可能在甲亢患者中增加,无论NO,以保护心血管系统免受ADMA可能的不良影响。关键词:不对称二甲基精氨酸,apelin,一氧化氮,甲状腺功能障碍DOI: 10.7176/JMPB/67-03出版日期:2020年8月31日
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引用次数: 0
COMPARISON OF POST OPERATIVE PAIN WITH EFFICACY OF INTRAVENOUS ACETAMINOPHEN VERSUS KETAMINE AMONG PATIENTS UNDERGOING ABDOMINAL HYSTERECTOMY 腹式子宫切除术患者术后疼痛及静脉注射对乙酰氨基酚与氯胺酮的疗效比较
Pub Date : 2020-08-01 DOI: 10.7176/jmpb/67-04
Ikram Umar Buttar, M. Ikram, M. Kashif
Objective; To determine the effectiveness in terms of postoperative pain with intravenous acetaminophen versus ketamine among women undergoing abdominal hysterectomy. Material and Methods; A total of 114 women undergoing abdominal hysterectomy were taken in this study. The study was conducted at Akhtar Saeed Trust Hospital, Lahore and DHQ Hospital Dera Ghazi Khan. Data was analyzed with statistical analysis program (SPSS version 21). Analysis was done to compare proportion of acetaminophen group and ketamine group. Chi-square test was applied to compare both groups in terms of efficacy taken p ≤0.05 as significant. Results; Our study comprised of 114 ladies undergoing abdominal hysterectomy, mean age of the study cases was 54.13 ± 6.89 years (with minimum age of the patients was 39 years while maximum age was 68 years). Majority of our study cases i.e. 92 (80.7 %) had parity more than 3 and American Society of Anesthesiology (ASA) score level 1 in 93 (81.6%). Sixty one (53.1%) belonged to rural areas, while mean duration of surgery was 72.80 ± 11.32 minutes. Mean BMI level in our study cases was noted to be 23.98 ±4.17 Kg/m 2 and obesity was present in 34 (29.8%) of our study cases. Efficacy was noted in 60 (52.6 %) of our study cases. Efficacy was 73.7% study cases in group A while 18 (31.6%) in group B (p=0.000). Conclusion; Our study results support the use of intravenous acetaminophen (IVA) for pain management in patients undergoing abdominal hysterectomy compared with ketamine. Intravenous acetaminophen is safe, reliable and effective for the pain management in postoperative abdominal hysterectomy patients which was associated with shorter hospital stay and cost effective for the suffering families. No adverse reactions were noted in this study with the use of intravenous acetaminophen. Keywords; Intravenous acetaminophen, Ketamine, Abdominal Hysterectomy. DOI: 10.7176/JMPB/67-04 Publication date: August 31 st 2020
客观的;目的:探讨对乙酰氨基酚与氯胺酮对腹部子宫切除术妇女术后疼痛的影响。材料与方法;本研究共收集了114例接受腹部子宫切除术的妇女。这项研究是在拉合尔的Akhtar Saeed信托医院和Dera Ghazi Khan DHQ医院进行的。数据分析采用统计分析程序(SPSS version 21)。分析比较对乙酰氨基酚组与氯胺酮组的比例。两组疗效比较采用卡方检验,p≤0.05为差异有统计学意义。结果;我们的研究包括114例接受腹部子宫切除术的女性,研究病例的平均年龄为54.13±6.89岁(最小年龄39岁,最大年龄68岁)。我们的大多数研究病例,即92例(80.7%)的胎次大于3,93例(81.6%)的美国麻醉学会(ASA)评分为1。农村61例(53.1%),平均手术时间72.80±11.32 min。我们研究病例的平均BMI水平为23.98±4.17 Kg/ m2, 34例(29.8%)存在肥胖。我们的研究病例中有60例(52.6%)有效率。A组有效率为73.7%,B组有效率为18例(31.6%)(p=0.000)。结论;我们的研究结果支持与氯胺酮相比,静脉注射对乙酰氨基酚(IVA)用于腹部子宫切除术患者的疼痛管理。静脉注射对乙酰氨基酚治疗腹部子宫切除术后疼痛安全、可靠、有效,缩短住院时间,对患者家属具有成本效益。本研究未发现静脉注射对乙酰氨基酚的不良反应。关键字;静脉注射对乙酰氨基酚,氯胺酮,腹部子宫切除术。DOI: 10.7176/JMPB/67-04出版日期:2020年8月31日
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引用次数: 0
PATTERN AND BURDEN OF ANTIMICROBIAL RESISTANCE AMONG CAUSATIVE ORGANISMS OF SPONTANEOUS BACTERIAL PERITONITIS. 自发性细菌性腹膜炎病原菌的耐药性模式和负担。
Pub Date : 2020-08-01 DOI: 10.7176/jmpb/67-07
M. Ikram, Ikram Umar Buttar, M. Kashif
Background; Spontaneous bacterial peritonitis is a common bacterial infection in patients with cirrhosis and ascities with in-hospital mortality rates ranging from 20 to 30%. Early diagnosis and a prompt antibiotic therapy have considerably decreased the mortality rate and this study was done to determine drug resistance pattern among causative organisms of SBP. Material and Methods; All the cases (n = 183) of Cirrhosis of liver with ascities, fulfilling inclusion criteria were recruited from Department of Medicine Akhtar Saeed Trust Hospital Lahore. All selected patients were subjected to ascitic fluid tap, under aseptic measures by inserting a needle of 22 / 18 gauge in the left flank of abdomen. Ten ml of ascitic fluid was aspirated in a heparinised disposable syringe; which was immediately inoculated into blood culture bottle at bedside and send for bacterial culture and causative organisms was tested for their antibiotic drug resistance pattern. Data was entered and analyzed by computer program SPSS-18. Results; Of these 183 study cases, 103 (56.3%) were male patients while 80 (43.7%) were female patients. Mean age of our study cases was 51.73 ± 9.28 years. Previous history of use of antibiotics before 72 hours was noted in 103 (56.3%), anti – HCV was positive in 103 (56.3%) while HBs Ag was positive in 13.7% of our study cases. Among these study cases, E.coli was noted in 135 (73.8%), Staph. Aureus in 17.5% and Klebsiella was noted in 8.7%. Chloramphenicol was resistant in 24 (13.1%), ampicillin was resistant in 143 (78.1%), co – trimoxazole in 61.2%, ciprofloxacin in 55.7%, Ofloxacin in 56.3%, Cefotaxime was resistant in 78.1% and amoxicillin was resistant in 74.9%. Conclusion; High degree of drug resistance was observed among  these strains in our study which points towards well directed efforts of our health policy makers to adopt certain guidelines regarding use of these drugs in our population. Our study results have indicated that E.coli was major causative organisms among patients with spontaneous bacterial peritonitis having cirrhosis of liver followed by staphylococcus aureus and klebsiella. Keywords; Cirrhosis, spontaneous bacterial peritonitis, E. coli. DOI: 10.7176/JMPB/67-07 Publication date: August 31 st 2020
背景;自发性细菌性腹膜炎是肝硬化和腹膜炎患者常见的细菌感染,住院死亡率为20%至30%。早期诊断和及时的抗生素治疗大大降低了死亡率,本研究旨在确定SBP致病生物的耐药模式。材料与方法;所有符合纳入标准的肝硬化合并腹水病例(183例)均来自拉合尔Akhtar Saeed信托医院内科。所有入选的患者均在无菌条件下,通过在腹部左侧插入22 / 18号针头进行腹水抽吸。用肝素化一次性注射器抽吸腹水10ml;立即接种到床边的血培养瓶中,送去细菌培养,检测病原菌的抗生素耐药模式。数据由SPSS-18计算机程序输入和分析。结果;183例患者中,男性103例(56.3%),女性80例(43.7%)。研究病例的平均年龄为51.73±9.28岁。103例(56.3%)有72小时前使用抗生素史,103例(56.3%)抗HCV阳性,13.7%的HBs Ag阳性。其中大肠杆菌135例(73.8%)检出;葡萄球菌135例(73.8%)检出;金黄色菌占17.5%,克雷伯菌占8.7%。氯霉素耐药24例(13.1%),氨苄西林耐药143例(78.1%),复方新诺明耐药61.2%,环丙沙星耐药55.7%,氧氟沙星耐药56.3%,头孢噻肟耐药78.1%,阿莫西林耐药74.9%。结论;在我们的研究中观察到这些菌株具有高度耐药性,这表明我们的卫生政策制定者应作出有针对性的努力,采用有关在我国人口中使用这些药物的某些指导方针。我们的研究结果表明,在肝硬化自发性细菌性腹膜炎患者中,大肠杆菌是主要的致病菌,其次是金黄色葡萄球菌和克雷伯菌。关键字;肝硬化,自发性细菌性腹膜炎,大肠杆菌。DOI: 10.7176/JMPB/67-07出版日期:2020年8月31日
{"title":"PATTERN AND BURDEN OF ANTIMICROBIAL RESISTANCE AMONG CAUSATIVE ORGANISMS OF SPONTANEOUS BACTERIAL PERITONITIS.","authors":"M. Ikram, Ikram Umar Buttar, M. Kashif","doi":"10.7176/jmpb/67-07","DOIUrl":"https://doi.org/10.7176/jmpb/67-07","url":null,"abstract":"Background; Spontaneous bacterial peritonitis is a common bacterial infection in patients with cirrhosis and ascities with in-hospital mortality rates ranging from 20 to 30%. Early diagnosis and a prompt antibiotic therapy have considerably decreased the mortality rate and this study was done to determine drug resistance pattern among causative organisms of SBP. Material and Methods; All the cases (n = 183) of Cirrhosis of liver with ascities, fulfilling inclusion criteria were recruited from Department of Medicine Akhtar Saeed Trust Hospital Lahore. All selected patients were subjected to ascitic fluid tap, under aseptic measures by inserting a needle of 22 / 18 gauge in the left flank of abdomen. Ten ml of ascitic fluid was aspirated in a heparinised disposable syringe; which was immediately inoculated into blood culture bottle at bedside and send for bacterial culture and causative organisms was tested for their antibiotic drug resistance pattern. Data was entered and analyzed by computer program SPSS-18. Results; Of these 183 study cases, 103 (56.3%) were male patients while 80 (43.7%) were female patients. Mean age of our study cases was 51.73 ± 9.28 years. Previous history of use of antibiotics before 72 hours was noted in 103 (56.3%), anti – HCV was positive in 103 (56.3%) while HBs Ag was positive in 13.7% of our study cases. Among these study cases, E.coli was noted in 135 (73.8%), Staph. Aureus in 17.5% and Klebsiella was noted in 8.7%. Chloramphenicol was resistant in 24 (13.1%), ampicillin was resistant in 143 (78.1%), co – trimoxazole in 61.2%, ciprofloxacin in 55.7%, Ofloxacin in 56.3%, Cefotaxime was resistant in 78.1% and amoxicillin was resistant in 74.9%. Conclusion; High degree of drug resistance was observed among  these strains in our study which points towards well directed efforts of our health policy makers to adopt certain guidelines regarding use of these drugs in our population. Our study results have indicated that E.coli was major causative organisms among patients with spontaneous bacterial peritonitis having cirrhosis of liver followed by staphylococcus aureus and klebsiella. Keywords; Cirrhosis, spontaneous bacterial peritonitis, E. coli. DOI: 10.7176/JMPB/67-07 Publication date: August 31 st 2020","PeriodicalId":16339,"journal":{"name":"Journal of Medicine, Physiology and Biophysics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89937221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Knowledge, Attitude and Practice Among Type II Diabetic Patients in Ambo University Referral Hospital, Oromia, Ethiopia 埃塞俄比亚奥罗米亚安博大学转诊医院II型糖尿病患者的知识、态度和行为
Pub Date : 2020-06-01 DOI: 10.7176/jmpb/66-01
Agama Daba, Tamiru Yazew
Background: Diabetes is a chronic illness significantly affecting health of population throughout the world. In Africa, about 19.8 million adults are affected by diabetes and of these, more than 50% are from Nigeria, South Africa, Ethiopia and Tanzania. Knowledge is the greatest weapon in the fight against diabetes mellitus (DM). Self-management of patients with type two DM largely depends on the knowledge, attitude and practices of such people regarding the condition. Objective : The main objective of this study was to assess knowledge, attitude and practices of type II diabetes (T2DM) patients attending Ambo University referral hospital, Oromia, Ethiopia. Method : An institutional based cross sectional study was conducted among randomly selected 248 T2DM patients aged ≥30 years old from Ambo University Referral Hospital. Data regarding KAP of diabetic patients were collected using interview techniques. In addition, anthropometric data (body mass index and waist hip ratio) of the participants were collected and assessed. The tool for data collection contains socio demographic characteristics and medical factors. Descriptive statistics was used to set the KAP level among the respondents while logistic regression model was applied to test the association of KAP with various independent variables. Results: The mean of age among the respondents was 44(±7.2) years. Of 248 respondents, 105 (42.3%) were from rural. Among the total 248 respondents, about 111(44.8%), 117(47.2%) and 121(48.8%) were differentiated as not knowledgeable, having poor attitude and poor practice towards diabetes mellitus, respectively. Age, place of residence, level of education, average monthly income, history of diabetes and duration of living with DM were identified as factors affecting KAP towards diabetes. Both sex and occupational status were also factors affecting the knowledge level of T2DM patients, but not attitude and practice. Furthermore, knowledge level was affecting booth attitude and practice level of participants in the study area. Conclusion and Recommendation: This study found that there was a gap in knowledge, attitude and practice towards diabetes among T2DM patients in the study area. Therefore, a structured nutrition and health education should be given for T2DM patients through community based behavioral change communication to assist and improve the level of KAP towards DM in the study area. Keywords: Type 2 diabetes mellitus, Knowledge, Attitude, Practice, Adults DOI: 10.7176/JMPB/66-01 Publication date: June 30th 2020
背景:糖尿病是一种严重影响全球人口健康的慢性疾病。在非洲,约有1980万成年人患有糖尿病,其中50%以上来自尼日利亚、南非、埃塞俄比亚和坦桑尼亚。知识是对抗糖尿病最有力的武器。2型糖尿病患者的自我管理在很大程度上取决于这些人对病情的认识、态度和实践。目的:本研究的主要目的是评估在埃塞俄比亚奥罗米亚Ambo大学转诊医院就诊的2型糖尿病(T2DM)患者的知识、态度和行为。方法:在安博大学转诊医院随机选取248例年龄≥30岁的T2DM患者进行横断面研究。采用访谈法收集糖尿病患者的KAP数据。此外,收集和评估参与者的人体测量数据(身体质量指数和腰臀比)。数据收集工具包含社会人口特征和医学因素。采用描述性统计设置被调查者的KAP水平,采用logistic回归模型检验KAP与各自变量的相关性。结果:调查对象平均年龄44(±7.2)岁。248名受访者中,农村105人(42.3%)。在248名受访者中,分别有约111人(44.8%)、117人(47.2%)和121人(48.8%)被划分为对糖尿病不了解、态度差和行为差。年龄、居住地、受教育程度、平均月收入、糖尿病史和糖尿病患者生活时间是影响糖尿病KAP的因素。性别和职业状况也是影响T2DM患者知识水平的因素,但态度和行为不受影响。此外,知识水平对研究区域参与者的展台态度和实践水平有影响。结论与建议:本研究发现研究区T2DM患者对糖尿病的认识、态度和行为存在差距。因此,应通过以社区为基础的行为改变交流,对T2DM患者进行结构化的营养健康教育,以帮助和提高研究地区对DM的KAP水平。关键词:2型糖尿病,知识,态度,实践,成人DOI: 10.7176/JMPB/66-01出版日期:2020年6月30日
{"title":"Knowledge, Attitude and Practice Among Type II Diabetic Patients in Ambo University Referral Hospital, Oromia, Ethiopia","authors":"Agama Daba, Tamiru Yazew","doi":"10.7176/jmpb/66-01","DOIUrl":"https://doi.org/10.7176/jmpb/66-01","url":null,"abstract":"Background: Diabetes is a chronic illness significantly affecting health of population throughout the world. In Africa, about 19.8 million adults are affected by diabetes and of these, more than 50% are from Nigeria, South Africa, Ethiopia and Tanzania. Knowledge is the greatest weapon in the fight against diabetes mellitus (DM). Self-management of patients with type two DM largely depends on the knowledge, attitude and practices of such people regarding the condition. Objective : The main objective of this study was to assess knowledge, attitude and practices of type II diabetes (T2DM) patients attending Ambo University referral hospital, Oromia, Ethiopia. Method : An institutional based cross sectional study was conducted among randomly selected 248 T2DM patients aged ≥30 years old from Ambo University Referral Hospital. Data regarding KAP of diabetic patients were collected using interview techniques. In addition, anthropometric data (body mass index and waist hip ratio) of the participants were collected and assessed. The tool for data collection contains socio demographic characteristics and medical factors. Descriptive statistics was used to set the KAP level among the respondents while logistic regression model was applied to test the association of KAP with various independent variables. Results: The mean of age among the respondents was 44(±7.2) years. Of 248 respondents, 105 (42.3%) were from rural. Among the total 248 respondents, about 111(44.8%), 117(47.2%) and 121(48.8%) were differentiated as not knowledgeable, having poor attitude and poor practice towards diabetes mellitus, respectively. Age, place of residence, level of education, average monthly income, history of diabetes and duration of living with DM were identified as factors affecting KAP towards diabetes. Both sex and occupational status were also factors affecting the knowledge level of T2DM patients, but not attitude and practice. Furthermore, knowledge level was affecting booth attitude and practice level of participants in the study area. Conclusion and Recommendation: This study found that there was a gap in knowledge, attitude and practice towards diabetes among T2DM patients in the study area. Therefore, a structured nutrition and health education should be given for T2DM patients through community based behavioral change communication to assist and improve the level of KAP towards DM in the study area. Keywords: Type 2 diabetes mellitus, Knowledge, Attitude, Practice, Adults DOI: 10.7176/JMPB/66-01 Publication date: June 30th 2020","PeriodicalId":16339,"journal":{"name":"Journal of Medicine, Physiology and Biophysics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85826569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Assessment of Intension to Voluntary HIV Counseling and Testing Utilization and Associated Factors Among Youth Living in Mizan Aman Town, South-West Ethiopia By Health Belief Model, A Cross Sectional Study 基于健康信念模型的埃塞俄比亚西南部Mizan Aman镇青少年自愿接受艾滋病咨询检测意愿及相关因素评估
Pub Date : 2020-06-01 DOI: 10.7176/jmpb/66-03
Medhanit Worku Zewude
Background : - Voluntary Counseling and Testing (VCT) services have become one of the most common means of preventing, detecting and improving access to care and support to HIV/AIDS. Currently in Ethiopia, young people are most affected group of HIV/AIDS. Moreover, youth VCT utilization is low in studies among university students. Therefore, this study was aimed to assess youth’s voluntary counseling and testing utilization and associated factors in Mizan-Aman Town, Southwest Ethiopia. Community based cross sectional study was conducted among youth living in Mizan-Aman Twon. The data was collected using pre-tested interviewer administrating questionnaires using simple random sampling technique. Descriptive statistics, bivariate analysis, multivariate logistic regressions ware used accordingly. A total of 389 youths responded the questionnaire making the response rate of 96.6%. Two hundred thirty five (60.4%) were female. VCT utilization was 42.4% (95% CI=37.8%, 47.6%). Factor associated with intension to VCT service utilization are females [AOR=1.69, 95%CI= (1.017-2.814)]; attending secondary education level (AOR=2.099, 95%CI=(1.12-3.929)) and attending college education level (AOR=3.39, 95%CI=(1.783-6.45)); And having low perceived barrier and cue to action are [AOR=2.63,95%CI (1.236-5.614) and [AOR=10.85, 95 % CI=(6.05-19.46)] respectively. Being female, educated, high perceived barrier and cue to action were identified as significant associated factors for intension to utilize voluntary counseling and testing. More emphases should be given by concerned bodies in Mizan-Aman town health unit, Mizan Aman Health Science College on information translation regarding VCT, focus on male youth, collaborating with educational sectors, and assuring youth to benefit more on VCT services. Keywords: Voluntary Counseling and Testing (VCT), Mizan-Aman, Youth. DOI: 10.7176/JMPB/66-03 Publication date: June 30th 2020
背景:自愿咨询和检测(VCT)服务已成为预防、检测和改善获得艾滋病毒/艾滋病护理和支持的最常见手段之一。目前在埃塞俄比亚,年轻人是受艾滋病毒/艾滋病影响最大的群体。此外,青少年VCT在大学生研究中的使用率较低。因此,本研究旨在评估埃塞俄比亚西南部Mizan-Aman镇青少年自愿咨询和检测的使用情况及其相关因素。本研究对居住在米赞安曼镇的青少年进行了以社区为基础的横断面研究。数据的收集采用预先测试的采访者管理问卷,采用简单的随机抽样技术。描述性统计,双变量分析,多变量逻辑回归的应用。共有389名青少年回应问卷,回复率为96.6%。235例(60.4%)为女性。VCT使用率为42.4% (95% CI=37.8%, 47.6%)。与VCT服务使用强度相关的因素为女性[AOR=1.69, 95%CI= (1.017-2.814)];中等教育程度(AOR=2.099, 95%CI=(1.12-3.929))和大学教育程度(AOR=3.39, 95%CI=(1.783-6.45));低感知障碍和低动作提示分别为[AOR=2.63,95%CI(1.236 ~ 5.614)]和[AOR=10.85, 95%CI =(6.05 ~ 19.46)]。女性、受教育程度高、认知障碍和行动暗示被确定为利用自愿咨询和检测的意向的重要相关因素。米赞阿曼镇卫生单位、米赞阿曼健康科学学院的有关机构应更加重视有关VCT的信息翻译,关注男性青年,与教育部门合作,并确保青年更多地受益于VCT服务。关键词:自愿咨询与检测(VCT),米赞阿曼,青年。DOI: 10.7176/JMPB/66-03出版日期:2020年6月30日
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引用次数: 0
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Journal of Medicine, Physiology and Biophysics
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