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Osteolipoma of the oral cavity: Case report and review of literature 口腔骨脂瘤:1例报告及文献复习
Q3 Medicine Pub Date : 2023-01-01 DOI: 10.4103/jmau.jmau_6_23
A. Anjali, Subraj J Shetty, S. Gotmare, T. Pereira
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引用次数: 0
Histological Assessment of Systemic Toxicity Induced by Zinc oxide Nanoparticles and the Prophylactic Potency of Ascorbate in Albino Rats. 氧化锌纳米颗粒对白化大鼠全身毒性的组织学评价及抗坏血酸的预防作用。
Q3 Medicine Pub Date : 2022-12-01 eCollection Date: 2024-10-01 DOI: 10.4103/jmau.jmau_68_22
Amira Osman, Said Mohamed Afify, Amira Frag, Sarah Mohammed Alghandour

Background: Nanoparticles of zinc oxide (ZnO-NPs) are frequently implemented in cosmetics, additives, and electronic devices. Moreover, their applications extend to water treatment, drug delivery, and cancer therapy. As a result, NP toxicity became an essential subject in biosafety research.

Aim: Using histological and immunohistochemical analysis, we attempted to investigate whether ascorbate ("vitamin C") (VC) could protect liver, lung, and spleen tissues from ZnO-NP systemic toxicity.

Materials and methods: Rats were classified as control group, NP group injected intraperitoneally (IP), once by dissolved ZnO-NPs (200 mg/kg), and NP + VC group injected IP, once by dissolved ZnO-NPs (200 mg/kg) and then ingested 100 mg/kg of VC orally. Blood samples were collected. Liver, lung, and spleen specimens were prepared for light, electron microscopic, and immunohistochemical analysis.

Results: In comparison to the control group, the NP group's liver enzyme, i.e. aspartate transaminase and alanine transaminase, values and counts of white blood cells (WBCs) were higher on the 7th day, but their red blood corpuscle (RBC) count, hemoglobin (Hgb) level, platelet count, and albumin values were lower. Histopathological analysis of liver, lung, and spleen tissues showed severe toxicity manifested by cell apoptosis, mononuclear cell infiltration, dilated blood vessels, and hemorrhage. In addition, the NP group showed a significantly higher expression of Ki67 and caspase-3 immunoreactivity. The biochemical, hematological, and histopathological results of the NP + VC group improved overall, reflecting VC's protective effect against systemic toxicity.

Conclusion: Our study revealed that ascorbate (VC) inhibited the systemic toxicity prompted by ZnO-NPs in lung, liver, and spleen tissues, indicating its importance for future treatment with ZnO-NPs.

背景:氧化锌纳米颗粒(ZnO-NPs)经常应用于化妆品、添加剂和电子设备中。此外,它们的应用还扩展到水处理、药物输送和癌症治疗。因此,NP毒性已成为生物安全研究的重要课题。目的:通过组织学和免疫组织化学分析,探讨抗坏血酸(“维生素C”)(VC)是否具有保护肝脏、肺和脾脏组织免受ZnO-NP全身毒性的作用。材料和方法:将大鼠分为对照组,NP组腹腔注射一次,溶解ZnO-NPs (200 mg/kg); NP + VC组腹腔注射一次,溶解ZnO-NPs (200 mg/kg),然后口服VC 100 mg/kg。采集了血液样本。制备肝脏、肺和脾脏标本进行光镜、电镜和免疫组织化学分析。结果:与对照组相比,NP组第7天的肝酶(天冬氨酸转氨酶、丙氨酸转氨酶)值和白细胞(wbc)计数较高,红细胞(RBC)计数、血红蛋白(Hgb)水平、血小板计数和白蛋白值较低。肝、肺、脾组织病理分析显示严重毒性,表现为细胞凋亡、单核细胞浸润、血管扩张、出血。此外,NP组Ki67和caspase-3的免疫反应性显著升高。NP + VC组的生化、血液学和组织病理学结果总体改善,反映了VC对全身毒性的保护作用。结论:我们的研究表明,抗坏血酸(VC)可以抑制ZnO-NPs引起的肺、肝和脾组织的全身毒性,这表明VC对未来使用ZnO-NPs治疗具有重要意义。
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引用次数: 0
ImageJ-FIJI-Assisted Estimation of Intestinal Layers' Volume: Study in Jejunum-Ileum of Rats. image - fiji辅助估计大鼠空肠-回肠肠层体积的研究。
Q3 Medicine Pub Date : 2022-12-01 eCollection Date: 2024-10-01 DOI: 10.4103/jmau.jmau_53_22
Satrio Adi Wicaksono, Yustina Andwi Ari Sumiwi, Dewi Kartikawati Paramita, Rina Susilowati

Background: Studies in the digestive tract often required precision quantification of intestinal volume to observe the effect of certain intervention/condition. Application of stereological methods could bring unbiased and accurate results but commercially computer-assisted systems are not widely available. ImageJ-FIJI is an open source software, which could become an alternative choice in the stereological measurement process.

Aim: This study describes simple stereological quantification methods during volume estimation of jejunum-ileum intestinal layers of the rats using a light microscope and ImageJ-FIJI stereological tool.

Material and methods: Six 3-months old male Sprague-Dawley rats were terminated and jejunum-ileum was harvested after perfusion. After removal of intestinal luminal content, whole jejunum-ileum weight was measured. The organ was sampled as 6-10 slabs of 1 cm length in a systematic uniformed random sampling manner. Slabs were cut longitudinally at random angles before flattened and put on filter papers for subsequent tissue processing into 2-3 paraffin blocks. One section of 3 μm thick was sampled from each block, stained using toluidine blue and documented using a light microscope connected to a microstepper apparatus. The volume of the intestinal layers was estimated using a point-counting grid on Image J-FIJI software.

Result: We compared two sets of counting methods i.e. minimal counting (MC) and rigorous counting (RC) approaches that differ in their respective a/p value. Quantification using RC approach resulted in significantly higher estimated volume of tunica submucosa and tunica muscularis while having more preferable stereological accuracy parameters (CE<5% & CV<10%).

Conclusion: Although it required longer counting time, rigorous approaches resulted in higher accuracy while still within the range of rule of thumb criteria of 0.2 < CE2/CV2 < 0.5.

背景:消化道研究往往需要精确定量肠容积来观察某种干预/条件的效果。应用立体学方法可以带来公正和准确的结果,但商用计算机辅助系统并不广泛使用。imagj - fiji是一个开源软件,它可以成为立体测量过程中的另一种选择。目的:介绍利用光学显微镜和imagj - fiji立体工具对大鼠空肠-回肠肠层进行体积估算的简单立体定量方法。材料与方法:处死6只3月龄雄性sd大鼠,灌注后取空肠-回肠。去除肠管内容物后,测量全空肠-回肠重量。以系统均匀随机抽样的方式,将器官取样为6-10块长度为1cm的薄片。将板坯按任意角度纵向切割,然后压平,放在滤纸上进行后续组织处理,切成2-3块石蜡。每个块取3 μm厚的切片,用甲苯胺蓝染色,用光学显微镜连接微步进仪记录。使用Image J-FIJI软件上的点计数网格估计肠层的体积。结果:我们比较了两组计数方法,即最小计数(MC)和严格计数(RC)方法,它们各自的a/p值不同。使用RC方法定量可以显著提高粘膜下层和肌肉层的估计体积,同时具有更好的立体学精度参数(cec结论:虽然需要更长的计数时间,但严格的方法可以提高精度,同时仍在0.2 < CE2/CV2 < 0.5的经验法则标准范围内。
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引用次数: 0
Categorizing Malignant Small Round Cell Tumors in Aspiration Cytology: An Institutional Experience. 抽吸细胞学中恶性小圆形细胞瘤的分类:机构经验。
Q3 Medicine Pub Date : 2022-12-01 eCollection Date: 2024-01-01 DOI: 10.4103/jmau.jmau_66_21
Nibedita Sahoo, Urvashi Ghosh, Debahuti Mohapatra, Priyadarshini Dehuri

Aim and objectives: The study aims to categorize malignant small round cell tumors (MSRCTs) originating in various sites of the body with the objective of utilization of cytomorphological features and ancillary techniques.

Study design: It is a cross-sectional study conducted over a time span of 3 years (2017-2020). 33 cases of tumors with round cell morphology were evaluated by fine needle aspiration cytology (FNAC).

Materials and methods: The application of cell block preparation supported by immunohistochemistry aided in the categorization of 23 cases with definite diagnosis and the rest were reported as MSRCTs.

Results: Among the categorized 23/33 cases, the most common diagnosis was Ewing's sarcoma (7/23) followed by 6 cases of lymphoma. There were 2 cases each of rhabdomyosarcoma and Langerhans cell histiocytosis (LCH) and 1 case each of neuroblastoma, desmoplastic small round cell tumor (DSRCT), myeloid sarcoma, neuroendocrine tumor of pancreas, plasmacytoma, and small cell carcinoma. Histopathology confirmation was available in 24/33 cases. Among the categorized tumors (23/33), biopsy correlation was available in 19 cases, of which concordant result was seen in 17 cases (89.47%), which were 6 cases of lymphoma, 5 cases of Ewing's sarcoma (EWS), 2 of rhabdomyosarcoma, and 1 each of neuroblastoma, small cell carcinoma, DSRCT, and LCH. Discordant result was seen in one case of rhabdomyosarcoma and a case of synovial sarcoma reported as extraskeletal EWS in cytology. Out of the uncategorized cases reported as MSRTCs, histopathology was available in 5 cases which were diagnosed as rhabdomyosarcoma (1 cases), lymphoma (1 case), amelanotic melanoma (1 case), and extraskeletal EWS (2 cases).

Conclusion: Categorization of MSRCTs should be done to implement appropriate therapeutic protocol. FNAC provides a rapid diagnosis contributing immensely for the timely management of the patient. Detailed cytomorphological evaluation serves as a guide for further evaluation by ancillary techniques leading to definitive diagnosis.

目的和目标本研究旨在利用细胞形态学特征和辅助技术,对起源于身体不同部位的恶性小圆形细胞肿瘤(MSRCTs)进行分类:这是一项横断面研究,时间跨度为3年(2017-2020年)。通过细针穿刺细胞学(FNAC)对33例圆形细胞形态的肿瘤进行评估:应用细胞块制备并辅以免疫组化技术,对 23 例病例进行了明确诊断分类,其余病例被报告为 MSRCTs:在已分类的 23/33 个病例中,最常见的诊断是尤文氏肉瘤(7/23),其次是 6 例淋巴瘤。横纹肌肉瘤和朗格汉斯细胞组织细胞增生症(Langerhans cell histiocytosis,LCH)各占 2 例,神经母细胞瘤、去瘤小圆细胞瘤(Desmoplastic small round cell tumor,DSRCT)、髓系肉瘤、胰腺神经内分泌瘤、浆细胞瘤和小细胞癌各占 1 例。组织病理学证实的病例有 24/33 例。在已分类的肿瘤(23/33)中,19 例有活检相关性,其中 17 例(89.47%)结果一致,包括 6 例淋巴瘤、5 例尤文氏肉瘤(EWS)、2 例横纹肌肉瘤,以及神经母细胞瘤、小细胞癌、DSRCT 和 LCH 各 1 例。有一例横纹肌肉瘤和一例滑膜肉瘤在细胞学中被报告为骨骼外 EWS,结果不一致。在报告为 MSRTC 的未分类病例中,有 5 例获得了组织病理学诊断,分别被诊断为横纹肌肉瘤(1 例)、淋巴瘤(1 例)、黑色素瘤(1 例)和骨外 EWS(2 例):结论:应将 MSRCT 分类,以实施适当的治疗方案。FNAC可提供快速诊断,为及时治疗患者做出巨大贡献。详细的细胞形态学评估可为辅助技术的进一步评估提供指导,最终明确诊断。
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引用次数: 0
Primary Sacral Activated B-Cell Like Diffuse Large B-Cell Lymphoma, Triple Expressor Type: A Case Report with Literature Review. 原发性骶骨活化b细胞样弥漫性大b细胞淋巴瘤,三重表达型:1例报告并文献复习。
Q3 Medicine Pub Date : 2022-12-01 eCollection Date: 2024-10-01 DOI: 10.4103/jmau.jmau_64_22
Debahuti Mohapatra, Pradipta Tripathy, Sandip Mohanty, Ankita Pal

Primary bone lymphomas are unusual, and accounts for 2% among all lymphomas. Primary sacral lymphomas are still rarer with only 12 cases reported till date. They predominantly affect elderly males, showing occasional spinal epidural space involvement. We report a case of 49-year-old male complaining of low backache and radicular pain for 2 months, followed by the development of parasthesia and bladder and bowel involvement. Lumbosacral spine magnetic resonance imaging revealed an expansile lytic lesion of approximately 6.5 cm with enhanced soft-tissue component extending to neural foramina compressing the spinal cord. Biopsy showed non-Hodgkin's lymphoma showing CD20, B-cell lymphoma (BCL) 6, BCL2, Multiple myeloma (MM) antigen 1 (MUM 1), and Cellular myelocytomatosis oncogene (C-Myc) positivity with CD10 negativity. Thus, the diagnosis of diffuse large B-cell lymphoma (DLBL)-activated B-cell type was rendered. Thirty cases of CD10-negative DLBL with Myc translocation in bone have been found which occur predominantly in Asians. Sacral primary DLBL with Myc translocation is extremely rare, only two cases are reported till date to the best of our knowledge. Because of its rarity, the prognosis and treatment of this entity are still not clear.

原发性骨淋巴瘤是罕见的,占所有淋巴瘤的2%。原发性骶骨淋巴瘤仍然是罕见的,只有12例报告到目前为止。它们主要影响老年男性,偶尔表现为脊髓硬膜外腔受累。我们报告一例49岁男性主诉腰痛和神经根性疼痛2个月,随后发展为感觉异常和膀胱及肠道受累。腰骶椎骨磁共振成像显示约6.5 cm的扩张性溶解性病变,软组织成分增强,延伸至压迫脊髓的神经孔。活检显示非霍奇金淋巴瘤CD20、b细胞淋巴瘤(BCL) 6、BCL2、多发性骨髓瘤(MM)抗原1 (MUM 1)和细胞髓细胞瘤癌基因(C-Myc)阳性,CD10阴性。因此,弥漫性大b细胞淋巴瘤(DLBL)活化b细胞型的诊断被提出。已发现30例cd10阴性DLBL伴Myc骨易位,主要发生在亚洲人。骶骨原发性DLBL伴Myc易位是极为罕见的,据我们所知至今仅有2例报道。由于其罕见,预后和治疗仍不清楚。
{"title":"Primary Sacral Activated B-Cell Like Diffuse Large B-Cell Lymphoma, Triple Expressor Type: A Case Report with Literature Review.","authors":"Debahuti Mohapatra, Pradipta Tripathy, Sandip Mohanty, Ankita Pal","doi":"10.4103/jmau.jmau_64_22","DOIUrl":"10.4103/jmau.jmau_64_22","url":null,"abstract":"<p><p>Primary bone lymphomas are unusual, and accounts for 2% among all lymphomas. Primary sacral lymphomas are still rarer with only 12 cases reported till date. They predominantly affect elderly males, showing occasional spinal epidural space involvement. We report a case of 49-year-old male complaining of low backache and radicular pain for 2 months, followed by the development of parasthesia and bladder and bowel involvement. Lumbosacral spine magnetic resonance imaging revealed an expansile lytic lesion of approximately 6.5 cm with enhanced soft-tissue component extending to neural foramina compressing the spinal cord. Biopsy showed non-Hodgkin's lymphoma showing CD20, B-cell lymphoma (BCL) 6, BCL2, Multiple myeloma (MM) antigen 1 (MUM 1), and Cellular myelocytomatosis oncogene (C-Myc) positivity with CD10 negativity. Thus, the diagnosis of diffuse large B-cell lymphoma (DLBL)-activated B-cell type was rendered. Thirty cases of CD10-negative DLBL with Myc translocation in bone have been found which occur predominantly in Asians. Sacral primary DLBL with Myc translocation is extremely rare, only two cases are reported till date to the best of our knowledge. Because of its rarity, the prognosis and treatment of this entity are still not clear.</p>","PeriodicalId":16340,"journal":{"name":"Journal of Microscopy and Ultrastructure","volume":"1 1","pages":"228-231"},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11729018/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70801410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Phenotypic and Molecular Spectrum of Guanidinoacetate N-Methyltransferase Deficiency: An Analytical Study of a Case Series and a Scoping Review of 53 Cases of Guanidinoacetate N-Methyltransferase. 胍乙酸 N-甲基转移酶缺乏症的表型和分子谱:胍基乙酸 N-甲基转移酶缺乏症的表型和分子谱:一个病例系列的分析研究和 53 例胍基乙酸 N-甲基转移酶缺乏症病例的范围回顾。
Q3 Medicine Pub Date : 2022-12-01 eCollection Date: 2024-04-01 DOI: 10.4103/jmau.jmau_16_22
Anas S Alyazidi, Osama Y Muthaffar, Mohammed K Shawli, Renad A Ahmed, Yara Fahad Aljefri, Layan Saleh Baaishrah, Abdulaziz T Jambi, Fahad A Alotibi

Background: Guanidinoacetate methyltransferase deficiency (GAMT) is an autosomal recessive inborn error of metabolism. A condition that results from a pathogenic variant in the GAMT gene that maps to 19p13.3. The prevalence can be estimated to be up to 1:2,640,000 cases; countries such as Saudi Arabia could have a higher prevalence due to high consanguinity rates. The clinical manifestations that a patient could obtain are broad and start to manifest in the patients' early childhood years.

Materials and methods: A thorough review of case reports in January 2022 was conducted. The retrieved literature was screened for demographic data. Patients of all ages were included. Qualitative variables were described as number and percentage (%), and quantitative data were described by the mean and standard deviation. In bivariate data, Chi-square test (χ2) was used and t-test for nonparametric variables.

Results: Gender distribution was 53% of males and 47% females. Reported age ranged from 8 to 31 months. At the age of onset, 50% of the cases were infants, 28% were toddlers, and 15% were children, concluding that 79% of the reported cases developed symptoms before 5 years old. 68% of the cases developed generalized seizures throughout their life. 84% of the cases expressed a form of developmental delay. 43% of the cases had intellectual disabilities and mental retardation that affected their learning process; most cases required special care. 23% of the affected cases were of consanguineous marriages, and 7% had affected relatives.

Conclusion: We described four novel case reports, the first to be reported in Saudi Arabia. Seizure was a leading finding in the majority of the cases. Developmental delay was broadly observed. Intellectual delay and language impairments are primary hallmarks. Further understanding and early diagnosis are recommended. Premarital testing of neurogenetic diseases using whole-exome sequencing is probably a future direction, especially in populations with high consanguinity rates.

背景:胍基乙酸甲基转移酶缺乏症(GAMT)是一种常染色体隐性遗传的先天性代谢错误。GAMT 基因的致病变体导致这种疾病,该基因映射到 19p13.3。据估计,该病的发病率高达 1:2,640,000;由于近亲结婚率较高,沙特阿拉伯等国家的发病率可能更高。患者可能出现的临床表现很广泛,在患者幼年时期就开始表现出来:对 2022 年 1 月的病例报告进行了全面审查。对检索到的文献进行了人口统计学数据筛选。所有年龄段的患者均包括在内。定性变量以数量和百分比(%)表示,定量数据以平均值和标准差表示。对于二元数据,采用卡方检验(χ2);对于非参数变量,采用 t 检验:性别分布:男性占 53%,女性占 47%。报告年龄从 8 个月到 31 个月不等。从发病年龄来看,50%的病例为婴儿,28%为幼儿,15%为儿童。68%的病例终生都会出现全身性癫痫发作。84%的病例表现出某种形式的发育迟缓。43%的病例有智力障碍和精神发育迟滞,影响了他们的学习过程;大多数病例需要特殊照顾。23%的病例为近亲结婚,7%的病例有患病亲属:我们描述了四例新的病例报告,这在沙特阿拉伯尚属首次。大多数病例的主要发现是癫痫发作。广泛观察到发育迟缓。智力发育迟缓和语言障碍是主要特征。建议进一步了解并及早诊断。利用全外显子组测序对神经遗传疾病进行婚前检测可能是未来的一个方向,尤其是在近亲结婚率较高的人群中。
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引用次数: 0
Peripheral Fibro-osseous Lesion: An Institutional Study of 38 Cases. 38例周围纤维性骨性病变的机构分析。
Q3 Medicine Pub Date : 2022-12-01 eCollection Date: 2024-10-01 DOI: 10.4103/jmau.jmau_67_22
Apeksha Jain, Rishikesh Mehta, Treville Pereira, Rutuja Gajanan Vidhale, A K Anjali, Subraj J Shetty

Background: The term "peripheral fibro-osseous lesion (PFOL)" is used relatively for common gingival lesions characterized histologically by hypercellular connective tissue showing either new bone-like formations or cementum-like substance and rarely dystrophic calcifications. These lesions are closely related to the other fibro-osseous lesions such as cemental periapical dysplasia, fibrous dysplasia, and other calcifying odontogenic cysts and tumors. The etiology is unknown, but certain authors suggest plaque, dental calculus, and ill-fitting dentures which might be the irritating agents causing irritation to the periodontal ligament which leads to such reactive growth.

Aim: The main aim of the present study is to present the clinicopathological features of a series of cases from the institute of D. Y Patil Deemed to be University, School of Dentistry.

Materials and methods: A total of 38 cases were evaluated over a period of 20 years. The age, size, gender, location, signs and symptoms, irritating agents, and the type of mineralized tissue present in the lesions were evaluated. Modified Gallego's Stain was performed to identify the hard tissues.

Results: The age range of patients (males = 23, females = 15) was 6-71 years (mean = 29.842 years). The lesions had more predisposition for males (males = 60.53%, females = 39.47%). The occurrence of the lesion was more in maxilla with 65% of cases occurring in anterior region.

Conclusion: According to the present study, PFOL has a slight predominance in males, with the mean age being 29.842 years with the highest occurrence in the anterior maxillary arch.

背景:“外周纤维-骨性病变(PFOL)”一词用于较为常见的牙龈病变,其组织学特征为细胞过多的结缔组织,表现为新的骨样形成或骨质样物质,很少有营养不良的钙化。这些病变与其他纤维骨性病变密切相关,如骨水泥尖周发育不良、纤维发育不良和其他钙化牙源性囊肿和肿瘤。病因尚不清楚,但某些作者认为,牙菌斑、牙石和不合适的假牙可能是刺激牙周韧带的刺激物,导致这种反应性生长。目的:本研究的主要目的是介绍来自被认为是大学牙科学院的D. Y . Patil研究所的一系列病例的临床病理特征。材料与方法:对20年来38例患者进行回顾性分析。评估患者的年龄、大小、性别、位置、体征和症状、刺激物以及病变中存在的矿化组织类型。改良Gallego染色法鉴定硬组织。结果:患者年龄6 ~ 71岁(男23例,女15例),平均29.842岁。男性易患该病变(男性= 60.53%,女性= 39.47%)。上颌病变多发,65%的病例发生在前牙区。结论:根据本研究,PFOL在男性中有轻微优势,平均年龄为29.842岁,上颌前弓发生率最高。
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引用次数: 0
Michelia Champaca L. Modulates Superoxide Dismutase and Apoptosis-Regulating Proteins in Hippocampus of Middle-Aged Female Rats. Michelia Champaca L. 可调节中年雌性大鼠海马中的超氧化物歧化酶和细胞凋亡调节蛋白。
Q3 Medicine Pub Date : 2022-12-01 eCollection Date: 2024-04-01 DOI: 10.4103/jmau.jmau_4_22
Alchalidi Alchalidi, Nora Veri, Emilda Emilda, Cut Mutiah, Magfirah Magfirah, Henniwati Henniwati, Meliani Sukmadewi Harahap, Elly Susilawati

Background and objective: The aging process in women is still a problem, especially in developing countries that will become developed countries, including Indonesia. Here, we investigated the action of Michelia champaca L. extract administration on antioxidant modulation to inhibit hippocampal apoptosis in middle-aged female rats.

Materials and methods: Thirty-two female Wistar rats were divided into four groups (n = 8 each group): the middle-aged rats without any treatment (control group) and three M. champaca L. extract groups (treated at doses of 100, 200, or 300 mg/kg b. w). The superoxide dismutase (SOD) levels and Bax and Bcl-2 expressions in the hippocampal region were analyzed using the technique of enzyme-linked immunosorbent assay.

Results: The second and third doses of M. champaca L. extract significantly increased the SOD hippocampal levels compared with the control (P < 0.05). This extract also decreased Bax expression (at the second and third doses) and significantly increased Bcl-2 expression (at the highest dose) than that of the control (P < 0.05).

Conclusions: The ethanol extract of M. champaca L. could modulate SOD and regulate apoptotic-related proteins in middle-aged female rats. Thus, the extract of M. champaca L. can be an alternative to prevent the degeneration of hippocampus due to the aging process.

背景和目的:妇女的衰老过程仍然是一个问题,特别是在即将成为发达国家的发展中国家,包括印度尼西亚。在此,我们研究了中年雌性大鼠服用 Michelia champaca L. 提取物对抑制海马凋亡的抗氧化调节作用:将32只雌性Wistar大鼠分为4组(每组8只):未进行任何处理的中年大鼠组(对照组)和3组Michelia champaca L.提取物组(处理剂量为100、200或300 mg/kg体重)。采用酶联免疫吸附法分析了海马区超氧化物歧化酶(SOD)水平、Bax和Bcl-2的表达:结果:与对照组相比,第二和第三剂量的 M. champaca L. 提取物能显著提高海马区的 SOD 水平(P < 0.05)。与对照组相比,该提取物还能降低 Bax 的表达(第二和第三剂量),并显著增加 Bcl-2 的表达(最高剂量)(P < 0.05):结论:M. champaca L.乙醇提取物能调节中年雌性大鼠体内的 SOD 和凋亡相关蛋白。因此,M. champaca L.乙醇提取物可作为预防衰老过程导致的海马退化的替代品。
{"title":"<i>Michelia Champaca</i> L. Modulates Superoxide Dismutase and Apoptosis-Regulating Proteins in Hippocampus of Middle-Aged Female Rats.","authors":"Alchalidi Alchalidi, Nora Veri, Emilda Emilda, Cut Mutiah, Magfirah Magfirah, Henniwati Henniwati, Meliani Sukmadewi Harahap, Elly Susilawati","doi":"10.4103/jmau.jmau_4_22","DOIUrl":"10.4103/jmau.jmau_4_22","url":null,"abstract":"<p><strong>Background and objective: </strong>The aging process in women is still a problem, especially in developing countries that will become developed countries, including Indonesia. Here, we investigated the action of <i>Michelia champaca L</i>. extract administration on antioxidant modulation to inhibit hippocampal apoptosis in middle-aged female rats.</p><p><strong>Materials and methods: </strong>Thirty-two female Wistar rats were divided into four groups (<i>n</i> = 8 each group): the middle-aged rats without any treatment (control group) and three <i>M</i>. <i>champaca L</i>. extract groups (treated at doses of 100, 200, or 300 mg/kg b. w). The superoxide dismutase (SOD) levels and Bax and Bcl-2 expressions in the hippocampal region were analyzed using the technique of enzyme-linked immunosorbent assay.</p><p><strong>Results: </strong>The second and third doses of <i>M</i>. <i>champaca L</i>. extract significantly increased the SOD hippocampal levels compared with the control (<i>P</i> < 0.05). This extract also decreased Bax expression (at the second and third doses) and significantly increased Bcl-2 expression (at the highest dose) than that of the control (<i>P</i> < 0.05).</p><p><strong>Conclusions: </strong>The ethanol extract of <i>M</i>. <i>champaca L</i>. could modulate SOD and regulate apoptotic-related proteins in middle-aged female rats. Thus, the extract of <i>M</i>. <i>champaca L</i>. can be an alternative to prevent the degeneration of hippocampus due to the aging process.</p>","PeriodicalId":16340,"journal":{"name":"Journal of Microscopy and Ultrastructure","volume":"1 1","pages":"71-74"},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11245127/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70800837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Impact of Conjugated Linoleic Acid on Obesity and Its Association with Macrophage Recruitment: Experimental and Immunohistochemical Study. 共轭亚油酸对肥胖的影响及其与巨噬细胞招募的关系:实验和免疫组织化学研究。
Q3 Medicine Pub Date : 2022-11-14 eCollection Date: 2024-07-01 DOI: 10.4103/jmau.jmau_25_22
Asmaa Gaber Abdou, Mohamed AbdElfattah Bendary, Sara E Abdou, Ghada S Amer

Background: Conjugated linoleic acid (CLA) has been shown in humans and animals to have anti-adipose effects. The current study aims to assess the prophylactic and therapeutic impact of CLA and its effect on recruited macrophage type using immunohistochemistry against CD68 and CD 163.

Materials and methods: Forty adult male albino rats of local strain were included in the study and divided into control, CLA-supplemented, obese, CLA-prophylactic obese, and CLA-treated obese groups. Biopsies from visceral fat of the investigated groups were obtained and assessed for histopathological changes and immunohistochemical staining for CD68 and CD163.

Results: Obese group showed hypertrophied adipocytes and infiltration by inflammatory cells compared to other groups. The obese group showed a marked increase in the CD68 positivity compared with that in the control and CLA-supplemented groups. CLA-prophylactic and CLA-treated groups showed mild immune reaction with a significant decrease in CD68 positivity compared to the obese group. The obese group showed a significant decrease in the CD163 positivity compared with that in the control and CLA-supplemented groups.

Conclusions: Adipose tissue in obese is characterized by inflammation with more M1 than M2 macrophages. CLA could direct the recruited macrophages toward the anti-inflammatory subtype (M2) which encourages its beneficial effects in prophylaxis from obesity.

背景:共轭亚油酸(CLA)在人类和动物身上已被证明具有抗脂肪作用。本研究旨在使用针对 CD68 和 CD 163 的免疫组化方法评估 CLA 的预防和治疗作用及其对招募的巨噬细胞类型的影响:40只当地品系成年雄性白化大鼠,分为对照组、CLA补充组、肥胖组、CLA预防性肥胖组和CLA治疗肥胖组。从研究组的内脏脂肪中获取活组织切片,评估组织病理学变化,并对 CD68 和 CD163 进行免疫组化染色:结果:与其他组相比,肥胖组脂肪细胞肥大,并有炎症细胞浸润。与对照组和补充 CLA 组相比,肥胖组的 CD68 阳性率明显升高。与肥胖组相比,CLA预防组和CLA处理组表现出轻微的免疫反应,CD68阳性率显著下降。与对照组和补充 CLA 组相比,肥胖组的 CD163 阳性率显著下降:结论:肥胖者脂肪组织的炎症特征是 M1 型巨噬细胞多于 M2 型巨噬细胞。结论:肥胖者的脂肪组织具有炎症特征,其中 M1 型巨噬细胞多于 M2 型巨噬细胞。CLA 可将招募的巨噬细胞导向抗炎亚型(M2),从而促进其在预防肥胖方面的有益作用。
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引用次数: 0
Is Programmed Death-Ligand 1 of Prognostic Significance in Triple-Negative Female Mammary Carcinoma? 程序性死亡配体 1 在三阴性女性乳腺癌中是否具有预后意义?
Q3 Medicine Pub Date : 2022-11-14 eCollection Date: 2024-01-01 DOI: 10.4103/jmau.jmau_77_21
Amal Mostafa Sanad, Wael Shawky Ibrahim, Iman Mohamed Ezzo, Rania Mohamed Sabry

Introduction: The most widespread female malignancy is breast cancer (BC), considerable percentage of patients with triple-negative BC (TNBC) experience rapid progression, recurrence, and metastasis. BC has not historically been treated as an immunogenic cancer. Nonetheless, several researchers have started to concentrate on immunotherapy.

Aim: The aim of the study is to investigate the immunohistochemical (IHC) expression of programmed death-ligand 1 (PD-L1) by stromal tumor-infiltrating lymphocytes (TILs) and tumor cells (TC) in female (TNBC) and to correlate with pathological features of such tumors, particularly those determine biologic behavior, such as the grade and stage the overall survival.

Methodology: This is a retrospective study which includes 49 paraffin-embedded tumor tissue sections which were collected from breast surgery specimens either radical or conservative of female patients with TNBC. The samples were analyzed immunohistochemically for PD-L1 expression.

Results: There were statistically significant relations among TC PD-L1 expression and TILs PD-L1 expression as well as relations among TILs PD-L1 expression with histologic grade, stromal TILs, and Ki-67 were statistically significant. Correlations between TC PD-L1 expression and N stage, histologic grade, and anatomic stage were statistically significant. Improved survival was detected within TILs PD-L1-positive cases; however, the correlation between the overall survival and PD-L1 expression in both TCs and stromal TIL was not statistically significant.

Conclusion: PD-L1 expressed in tumors with poor prognostic features such as the high grade, advanced T stage, and high Ki-67 index, TILs PD-L1-positive cases experienced improved survival supporting its prognostic significance. However, the correlation with overall survival was not statistically significant.

简介最常见的女性恶性肿瘤是乳腺癌(BC),相当比例的三阴性乳腺癌(TNBC)患者病情进展迅速、复发和转移。乳腺癌历来不被视为免疫原性癌症。目的:本研究旨在调查女性(TNBC)肿瘤基质浸润淋巴细胞(TILs)和肿瘤细胞(TC)表达程序性死亡配体1(PD-L1)的免疫组化(IHC)情况,并将其与此类肿瘤的病理特征,尤其是那些决定生物行为的特征(如分级和分期)以及总生存率相关联:这是一项回顾性研究,包括49份石蜡包埋的肿瘤组织切片,这些切片取自TNBC女性患者的根治性或保守性乳腺手术标本。对样本进行免疫组化分析,检测PD-L1的表达:结果:TC PD-L1表达与TILs PD-L1表达的关系有统计学意义,TILs PD-L1表达与组织学分级、基质TILs和Ki-67的关系也有统计学意义。TC PD-L1 表达与 N 分期、组织学分级和解剖分期之间的相关性具有统计学意义。在TIL PD-L1阳性病例中发现生存率有所提高;然而,总生存率与TC和基质TIL中PD-L1表达的相关性无统计学意义:结论:PD-L1在分级高、T期晚期、Ki-67指数高等预后不良的肿瘤中表达,PD-L1阳性的TILs可提高生存率,支持其预后意义。然而,与总生存期的相关性在统计学上并不显著。
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引用次数: 0
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