P. Herc, J. Čuboň, M. Čech, P. Haščík, Lukáš Jurčaga, M. Bobko, O. Bučko, Andrea Mesárošová, Jozef Kupec
The aim of the work was to analyze the effect of storage in freezing conditions (-18 ° C) during 3 months of storage on the formation of malondialdehyde MDA, TVB-N (total volatile basic nitrogen), and colour. Rainbow trout up to 500 g (RT) aged 1-1.5 years (n=15) and trout (RT1) up to 3.5 kg aged 2-2.5 years (n=15) were included in the experiment. The stated weight of the fish is already after processing. After slaughtering the fish, samples were taken from each fish for analysis of meat colour in the CIE colour space with a KONICA MINOLTA 2600D (L*, a*, b*) on the 1st day after slaughter and after the 1st, 2nd, 3rd month of storage. The content of TVB-N and MDA was measured parallel. For both weight categories of trout, after three months of freezing, we observed significant (RT1- P≤0.05; RT- P≤0.05) increase in TVB-N compared to the first day of measurement. Malondialdehyde content after three months of freezing was significantly (P≤0.05) higher than at the first measurement in both weight categories of rainbow trout. In the RT experimental group, trout meat after three months of freezing was significantly (P≤0.05) lighter (L*) and less yellow (b*). The meat of the RT1 group after 3 months of freezing storage was significantly (P≤0.05) darker , less red and less yellow.
{"title":"FAT OXIDATION, PROTEIN DEGRADATION AND COLOUR OF RAINBOW TROUT (ONCORHYNCHUS MYKISS) MEAT DURING 3 MONTHS OF FREEZER STORAGE","authors":"P. Herc, J. Čuboň, M. Čech, P. Haščík, Lukáš Jurčaga, M. Bobko, O. Bučko, Andrea Mesárošová, Jozef Kupec","doi":"10.55251/jmbfs.9196","DOIUrl":"https://doi.org/10.55251/jmbfs.9196","url":null,"abstract":"The aim of the work was to analyze the effect of storage in freezing conditions (-18 ° C) during 3 months of storage on the formation of malondialdehyde MDA, TVB-N (total volatile basic nitrogen), and colour. Rainbow trout up to 500 g (RT) aged 1-1.5 years (n=15) and trout (RT1) up to 3.5 kg aged 2-2.5 years (n=15) were included in the experiment. The stated weight of the fish is already after processing. After slaughtering the fish, samples were taken from each fish for analysis of meat colour in the CIE colour space with a KONICA MINOLTA 2600D (L*, a*, b*) on the 1st day after slaughter and after the 1st, 2nd, 3rd month of storage. The content of TVB-N and MDA was measured parallel. For both weight categories of trout, after three months of freezing, we observed significant (RT1- P≤0.05; RT- P≤0.05) increase in TVB-N compared to the first day of measurement. Malondialdehyde content after three months of freezing was significantly (P≤0.05) higher than at the first measurement in both weight categories of rainbow trout. In the RT experimental group, trout meat after three months of freezing was significantly (P≤0.05) lighter (L*) and less yellow (b*). The meat of the RT1 group after 3 months of freezing storage was significantly (P≤0.05) darker , less red and less yellow.","PeriodicalId":16348,"journal":{"name":"Journal of microbiology, biotechnology and food sciences","volume":" ","pages":""},"PeriodicalIF":0.9,"publicationDate":"2023-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45262235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
One of the essential elements in the in expression of recombinant proteins in Eshecichia coli is a promoter. It is a sequence of DNA that drives the expression of recombinant protein production. Along with the choice of promoter a number of factors such as protein parameters, induction temperature, inducer, induction conditions and protein solubility play a major role in deciding the yield of target protein. In the present study, we have evaluated the strength of three different promoters (phoA, cstA and pLpR) and compared it with one of the strong promoters known, T7. A vector with these three promoters were constructed for expression of FCTRX 1 – 15 tagged proinsulin gene. Protein expressed was isolated, solubilized and purified using chromatography. Yields of proinsulin obtained from different promoter driven constructs was compared and it was observed that the inclusion bodies (IB) yield obtained from temperature inducible pLpR system was comparable with T7 system and this can be further increased by optimizing the fermentation conditions.
{"title":"SCREENING OF VARIOUS PROMOTERS FOR INCREASED PROTEIN EXPRESSION","authors":"Nandini B NATARAJ, R. Sudhakaran","doi":"10.55251/jmbfs.9863","DOIUrl":"https://doi.org/10.55251/jmbfs.9863","url":null,"abstract":"One of the essential elements in the in expression of recombinant proteins in Eshecichia coli is a promoter. It is a sequence of DNA that drives the expression of recombinant protein production. Along with the choice of promoter a number of factors such as protein parameters, induction temperature, inducer, induction conditions and protein solubility play a major role in deciding the yield of target protein. In the present study, we have evaluated the strength of three different promoters (phoA, cstA and pLpR) and compared it with one of the strong promoters known, T7. A vector with these three promoters were constructed for expression of FCTRX 1 – 15 tagged proinsulin gene. Protein expressed was isolated, solubilized and purified using chromatography. Yields of proinsulin obtained from different promoter driven constructs was compared and it was observed that the inclusion bodies (IB) yield obtained from temperature inducible pLpR system was comparable with T7 system and this can be further increased by optimizing the fermentation conditions.","PeriodicalId":16348,"journal":{"name":"Journal of microbiology, biotechnology and food sciences","volume":" ","pages":""},"PeriodicalIF":0.9,"publicationDate":"2023-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46634174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This present paper is intended to demonstrate the desirable probiotic properties of Lactiplantibacillus plantarum TRG1 isolated from the Algerian goat rumen. The study focused on assessing the strain’s survival rate in stress conditions, namely in phenol 0.4%, bile salts 0.3%, in saliva and the Gastrointestinal tract (GIT) simulated conditions. The adhesion ability of the strain with GIT cells, cell surface properties, the production of EPS and β-galactosidase activity were also analyzed. Besides, biogenic amines production, sensitivity to antibiotics and hemolytic activity were evaluated as safety properties. The results showed that the strain can tolerate phenol 0.4% (13.88 ± 2.54%), bile salts 0.3% (10.91± 0.71%), and displayed good viability in saliva and different parts of the GIT. Viability rates were 99.26 % in saliva, 69.68 % in gastric juice, 37.4 % in the ileum. In the duodenum, 93.09 % of viability was attained after 90 min and 53.73% after 180 min of incubation. Furthermore, the strain displayed good adhesion and acceptable surface properties: hydrophobicity (76.94 ±3.36%), auto-aggregation (29.52% ± 6.95), and co-aggregation with E. coli (15.38 ± 05.43), P. aeruginosa (16.05 ± 2.14) and S. aureus (17.33 ± 2.31). A considerable amount of EPS (942.50 ± 32.78 mg/l) was noticed too. Results also showed that the strain did not present hemolytic activity while it can produce the β-galactosidase enzyme. The strain displaced sensitivity to nitroxolin and tetracycline and is resistant to other antibiotics.
{"title":"IN VITRO EVALUATION OF PROBIOTIC POTENTIAL OF Lactiplantibacillus plantarum TRG1 ISOLATED FROM AN ALGERIAN’S GOAT RUMEN","authors":"Tarek Khennouf, Amina Bouchefra, T. Idoui","doi":"10.55251/jmbfs.4278","DOIUrl":"https://doi.org/10.55251/jmbfs.4278","url":null,"abstract":"This present paper is intended to demonstrate the desirable probiotic properties of Lactiplantibacillus plantarum TRG1 isolated from the Algerian goat rumen. The study focused on assessing the strain’s survival rate in stress conditions, namely in phenol 0.4%, bile salts 0.3%, in saliva and the Gastrointestinal tract (GIT) simulated conditions. The adhesion ability of the strain with GIT cells, cell surface properties, the production of EPS and β-galactosidase activity were also analyzed. Besides, biogenic amines production, sensitivity to antibiotics and hemolytic activity were evaluated as safety properties. The results showed that the strain can tolerate phenol 0.4% (13.88 ± 2.54%), bile salts 0.3% (10.91± 0.71%), and displayed good viability in saliva and different parts of the GIT. Viability rates were 99.26 % in saliva, 69.68 % in gastric juice, 37.4 % in the ileum. In the duodenum, 93.09 % of viability was attained after 90 min and 53.73% after 180 min of incubation. Furthermore, the strain displayed good adhesion and acceptable surface properties: hydrophobicity (76.94 ±3.36%), auto-aggregation (29.52% ± 6.95), and co-aggregation with E. coli (15.38 ± 05.43), P. aeruginosa (16.05 ± 2.14) and S. aureus (17.33 ± 2.31). A considerable amount of EPS (942.50 ± 32.78 mg/l) was noticed too. Results also showed that the strain did not present hemolytic activity while it can produce the β-galactosidase enzyme. The strain displaced sensitivity to nitroxolin and tetracycline and is resistant to other antibiotics.","PeriodicalId":16348,"journal":{"name":"Journal of microbiology, biotechnology and food sciences","volume":" ","pages":""},"PeriodicalIF":0.9,"publicationDate":"2023-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43551021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Chňapek, Z. Gálová, Ž. Balážová, Z. Hromadová, Lucia Mikolášová, M. Vivodík, J. Drábeková, D. Rajnincová
The number of people suffering from food allergies and intolerances has been increasing in recent years and cereal proteins are the most common food allergens. Pseudocereals represent perspective alternative in nutrition with a positive effect on the human body. The aim of the work was to analyze the proteome of selected varieties of wheat (Triticum aestivum L.), oats (Avena sativa) and buckwheat (Fagopyrum esculentum Moench.) using two-dimensional electrophoresis (2DE) and mass spectrometry in order to detect the presence of potentially allergenic proteins. Using the PDQuest program, 221 protein spots ranging from 4.13 to 9.89 µl with experimental molecular weights from 12.42 kDa to 140 kDa were quantified in 2DE gels of wheat. In the oat sample, 168 protein spots were quantified in the range pI of 4.02 to 9.93 and an experimental molecular weight of 14.81 kDa to 67.96 kDa. Buckwheat proteins were separated on a 2DE gel into 208 protein spots in the 3 to 9.83 pI region with an experimental molecular weight of 10.10 kDa to 115 kDa. By comparing the data with the Allergome database, allergens Tri and 26, Tri and 33, Tri and 36, Tri and alpha Gliadin, Tri and 20 were detected in wheat, Ave s 11S allergens in oats and Fag e 1 allergen in buckwheat. 2DE together with mass spectrometry have been shown to be suitable and sensitive methods for the detection of allergens in food crops.
{"title":"APPLICATION OF TWO-DIMENSIONAL ELECTROPHORESIS AND MASS SPECTROMETRY FOR THE DETECTION OF ALLERGENS IN SELECTED VARIETIES OF WHEAT, OATS AND BUCKWHEAT","authors":"M. Chňapek, Z. Gálová, Ž. Balážová, Z. Hromadová, Lucia Mikolášová, M. Vivodík, J. Drábeková, D. Rajnincová","doi":"10.55251/jmbfs.9934","DOIUrl":"https://doi.org/10.55251/jmbfs.9934","url":null,"abstract":"The number of people suffering from food allergies and intolerances has been increasing in recent years and cereal proteins are the most common food allergens. Pseudocereals represent perspective alternative in nutrition with a positive effect on the human body. The aim of the work was to analyze the proteome of selected varieties of wheat (Triticum aestivum L.), oats (Avena sativa) and buckwheat (Fagopyrum esculentum Moench.) using two-dimensional electrophoresis (2DE) and mass spectrometry in order to detect the presence of potentially allergenic proteins. Using the PDQuest program, 221 protein spots ranging from 4.13 to 9.89 µl with experimental molecular weights from 12.42 kDa to 140 kDa were quantified in 2DE gels of wheat. In the oat sample, 168 protein spots were quantified in the range pI of 4.02 to 9.93 and an experimental molecular weight of 14.81 kDa to 67.96 kDa. Buckwheat proteins were separated on a 2DE gel into 208 protein spots in the 3 to 9.83 pI region with an experimental molecular weight of 10.10 kDa to 115 kDa. By comparing the data with the Allergome database, allergens Tri and 26, Tri and 33, Tri and 36, Tri and alpha Gliadin, Tri and 20 were detected in wheat, Ave s 11S allergens in oats and Fag e 1 allergen in buckwheat. 2DE together with mass spectrometry have been shown to be suitable and sensitive methods for the detection of allergens in food crops.","PeriodicalId":16348,"journal":{"name":"Journal of microbiology, biotechnology and food sciences","volume":" ","pages":""},"PeriodicalIF":0.9,"publicationDate":"2023-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42625493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ojochenemi Rebecca Egwumah, Kayode Rmo, B. I. Kayode, A. Abdulkadir
The by-product of soybean processing, soybean-hull, can have its fibre broken down by solid-state fermentation to improve digestibility and nutrient absorption. This research investigated the impacts of microbial strains in a solid-state fermentation on quality characteristics of soybean-hull. Soybean-hull was inoculated with a mono-culture of fungal (Aspergillus oryzae, Saccharomyces cerevisiae) and bacterial species (Bacillus subtilis, Lactobacillus plantarum) while unfermented soybean-hull served as control at (27±2oC) for 0hour, 24hours, 48hours and 72hours. At 24hours of fermentation, pH ranged from 6.17-6.42. TSS decreased significantly (p < 0.05) while TTA gradually increased in all samples. Soybean-hull with L plantarum at 24hours of fermentation had the highest value of iron (3.18 mg/l). Ca:P interactions were influenced, as there was an increase from 0.15 in the control to 3.45 in L. plantarum at 72hours. The protein (4.98-22.42%), lipid (3.58-21.04%), moisture (7.07-8.23%) increased significantly (P < 0.05) while carbohydrate (36.29-26.04%) and fibre (60.32-15.97%) decreased as fermentation progresses. Phytate and trypsin inhibitors reduced significantly. Fibre fractions of the fermented substrate decreased except NDS which increased. This study revealed that fermented soybean-hulls innoculated with Bacillus substilis and Aspergillus oryzae at 72hours offers better nutritional values and could be adopted as a new nutrient source.
{"title":"EFFECTS OF MICROBIAL STRAINS IN A SOLID-STATE FERMENTATION ON QUALITY ATTRIBUTES OF SOYBEAN-HULL","authors":"Ojochenemi Rebecca Egwumah, Kayode Rmo, B. I. Kayode, A. Abdulkadir","doi":"10.55251/jmbfs.9876","DOIUrl":"https://doi.org/10.55251/jmbfs.9876","url":null,"abstract":"The by-product of soybean processing, soybean-hull, can have its fibre broken down by solid-state fermentation to improve digestibility and nutrient absorption. This research investigated the impacts of microbial strains in a solid-state fermentation on quality characteristics of soybean-hull. Soybean-hull was inoculated with a mono-culture of fungal (Aspergillus oryzae, Saccharomyces cerevisiae) and bacterial species (Bacillus subtilis, Lactobacillus plantarum) while unfermented soybean-hull served as control at (27±2oC) for 0hour, 24hours, 48hours and 72hours. At 24hours of fermentation, pH ranged from 6.17-6.42. TSS decreased significantly (p < 0.05) while TTA gradually increased in all samples. Soybean-hull with L plantarum at 24hours of fermentation had the highest value of iron (3.18 mg/l). Ca:P interactions were influenced, as there was an increase from 0.15 in the control to 3.45 in L. plantarum at 72hours. The protein (4.98-22.42%), lipid (3.58-21.04%), moisture (7.07-8.23%) increased significantly (P < 0.05) while carbohydrate (36.29-26.04%) and fibre (60.32-15.97%) decreased as fermentation progresses. Phytate and trypsin inhibitors reduced significantly. Fibre fractions of the fermented substrate decreased except NDS which increased. This study revealed that fermented soybean-hulls innoculated with Bacillus substilis and Aspergillus oryzae at 72hours offers better nutritional values and could be adopted as a new nutrient source.","PeriodicalId":16348,"journal":{"name":"Journal of microbiology, biotechnology and food sciences","volume":" ","pages":""},"PeriodicalIF":0.9,"publicationDate":"2023-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45757891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Widiati Purnawita, W. P. Rahayu, H. Lioe, S. Nurjanah, Romsyah Maryam
Reuterin is a secondary metabolite of the lactic acid bacteria Limosilactobacillus reuteri having broad antimicrobial activity. This study aimed to determine the antifungal activity of reuterin against aflatoxigenic Aspergillus flavus (A. flavus). The aflatoxigenic A. flavus BIO 33212 isolated from Indonesian nutmeg was used in this study. The minimum inhibitory concentration (MIC) of reuterin based on radial growth and colony-forming assays in solid and liquid fat-enriched media against A. flavus was determined. The effect of reuterin on the morphological structure of A. flavus was investigated by scanning electron microscopy (SEM). The aflatoxin B1 (AFB1) produced by A. flavus was evaluated by enzyme-linked immunosorbent assay. Reuterin effectively prolonged the lag phase and decreased the growth rate of A. flavus. At 2 and 4 mM, reuterin demonstrated radial growth inhibition by 5.59% and 35.40%, respectively, while the reductions in colony numbers were 18.36% and 69.51%, respectively. At higher concentrations (6 and 8 mM), the fungal growth was inhibited and reduced completely (100%). SEM revealed the disruption of hyphae and conidiophore development and conidial damage. However, a contradictory phenomenon was found when reuterin at < 6 mM triggered a higher production of AFB1.
{"title":"ANTIFUNGAL ACTIVITY OF REUTERIN AGAINST AFLATOXIGENIC ASPERGILLUS FLAVUS","authors":"Widiati Purnawita, W. P. Rahayu, H. Lioe, S. Nurjanah, Romsyah Maryam","doi":"10.55251/jmbfs.10032","DOIUrl":"https://doi.org/10.55251/jmbfs.10032","url":null,"abstract":"Reuterin is a secondary metabolite of the lactic acid bacteria Limosilactobacillus reuteri having broad antimicrobial activity. This study aimed to determine the antifungal activity of reuterin against aflatoxigenic Aspergillus flavus (A. flavus). The aflatoxigenic A. flavus BIO 33212 isolated from Indonesian nutmeg was used in this study. The minimum inhibitory concentration (MIC) of reuterin based on radial growth and colony-forming assays in solid and liquid fat-enriched media against A. flavus was determined. The effect of reuterin on the morphological structure of A. flavus was investigated by scanning electron microscopy (SEM). The aflatoxin B1 (AFB1) produced by A. flavus was evaluated by enzyme-linked immunosorbent assay. Reuterin effectively prolonged the lag phase and decreased the growth rate of A. flavus. At 2 and 4 mM, reuterin demonstrated radial growth inhibition by 5.59% and 35.40%, respectively, while the reductions in colony numbers were 18.36% and 69.51%, respectively. At higher concentrations (6 and 8 mM), the fungal growth was inhibited and reduced completely (100%). SEM revealed the disruption of hyphae and conidiophore development and conidial damage. However, a contradictory phenomenon was found when reuterin at < 6 mM triggered a higher production of AFB1.","PeriodicalId":16348,"journal":{"name":"Journal of microbiology, biotechnology and food sciences","volume":" ","pages":""},"PeriodicalIF":0.9,"publicationDate":"2023-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45290188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Quorum sensing (QS) mechanism is cell communication that plays vital role in the development of infection by many pathogenic microorganisms. It controls multiple virulence factors such as pigmentation, biofilm formation, swarming motility, resistance towards antibiotics, extracellular polysaccharide production (EPS) and expression of several collective traits. The disruption of quorum sensing mechanism can be a solution to the emerging problem of multi-drug resistance among pathogenic bacteria. The effector molecule for Quorum sensing inhibition may be enzymatic or non- enzymatic in nature termed as Quorum Quenching (QQ) or Quorum sensing inhibitory (QSIs)/anti –QS compound, respectively. We used marine epibiotic bacteria as a source to obtained novel bacterial strain as QSI producer. One of the potent isolate, SJ4 was identified as Pseudomonas stutzeri SJ4, it is a short rod, gram negative bacterium. The ethyl acetate extract from P. stutzeri SJ4, showed highest QSI activity against monitor strain Chromobacterium violaceum (MTCC 5526). The extracted compound was tested against P. aeruginosa PAO1 at minimum inhibitiory concentration (MIC) and sub-MIC to study the effect on virulence factors. The significant inhibition of pycocyanin pigment, EPS production, rhamnolipid production and reduced swimming and swarming motility was observed. In addition, biofilm formation was notably inhibited which was confirmed by staining and spectometric method. Based on this observation, QS interption by extract which contain QSI remarkebly reduced the virulence of pathogen hence, can be use as therapeutic agents. The Thin Layer Chomatography (TLC) and Gas chrmoatography-Mass Spectrometry (GC-MS) identified major compound as n-Hexadecanoic acid. However, further research is required on purification of compound and its potential applications for the treatement of infections.
{"title":"EVALUATION OF ANTI-QUORUM SENSING ACTIVITY OF-HEXADECANOIC ACID PRODUCED BY PSEUDOMONAS STUTZERI SJ4 – A MARINE EPIBIOTIC BACTERIUM","authors":"Siddhi D. Shah, Nikita Vadadoriya, B. Bajpai","doi":"10.55251/jmbfs.5644","DOIUrl":"https://doi.org/10.55251/jmbfs.5644","url":null,"abstract":"Quorum sensing (QS) mechanism is cell communication that plays vital role in the development of infection by many pathogenic microorganisms. It controls multiple virulence factors such as pigmentation, biofilm formation, swarming motility, resistance towards antibiotics, extracellular polysaccharide production (EPS) and expression of several collective traits. The disruption of quorum sensing mechanism can be a solution to the emerging problem of multi-drug resistance among pathogenic bacteria. The effector molecule for Quorum sensing inhibition may be enzymatic or non- enzymatic in nature termed as Quorum Quenching (QQ) or Quorum sensing inhibitory (QSIs)/anti –QS compound, respectively. We used marine epibiotic bacteria as a source to obtained novel bacterial strain as QSI producer. One of the potent isolate, SJ4 was identified as Pseudomonas stutzeri SJ4, it is a short rod, gram negative bacterium. The ethyl acetate extract from P. stutzeri SJ4, showed highest QSI activity against monitor strain Chromobacterium violaceum (MTCC 5526). The extracted compound was tested against P. aeruginosa PAO1 at minimum inhibitiory concentration (MIC) and sub-MIC to study the effect on virulence factors. The significant inhibition of pycocyanin pigment, EPS production, rhamnolipid production and reduced swimming and swarming motility was observed. In addition, biofilm formation was notably inhibited which was confirmed by staining and spectometric method. Based on this observation, QS interption by extract which contain QSI remarkebly reduced the virulence of pathogen hence, can be use as therapeutic agents. The Thin Layer Chomatography (TLC) and Gas chrmoatography-Mass Spectrometry (GC-MS) identified major compound as n-Hexadecanoic acid. However, further research is required on purification of compound and its potential applications for the treatement of infections.","PeriodicalId":16348,"journal":{"name":"Journal of microbiology, biotechnology and food sciences","volume":" ","pages":""},"PeriodicalIF":0.9,"publicationDate":"2023-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42759696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Silvia Jakabová, J. Árvay, L. Benešová, P. Zajác, Jozef Čapla, J. Čurlej, J. Golian
Goat cheese and sheep cheese can offer a range of unique flavors and potential health benefits, making them popular choices among cheese lovers. Both types of cheese contain biogenic amines, which are naturally present in many types of food. The purpose of this study was to assess the levels of the seven most commonly occurring biogenic amines in semi-hard and hard goat and sheep cheeses intended for the Slovak market. The HPLC-DAD method with pre-column derivatization using dansylchloride was employed for the analysis of the hard and semi-hard cheese samples. The presence of biogenic amines was confirmed in all cheese samples, with total levels ranging from 14.4 mg.kg-1 to 1322 mg.kg-1. The most abundant compounds found in the samples were tyramine and tryptamine, followed by 2-phenylethylamine and spermine. Histamine was present in 54.5% of the samples, but its concentration did not exceed 68.7 mg.kg-1. Statistically significant differences in the levels of individual biogenic amines were observed between goat and sheep cheeses, except for spermidine and spermine.
{"title":"EVALUATION OF BIOGENIC AMINES IN GOAT AND SHEEP CHEESES OF SLOVAK ORIGIN","authors":"Silvia Jakabová, J. Árvay, L. Benešová, P. Zajác, Jozef Čapla, J. Čurlej, J. Golian","doi":"10.55251/jmbfs.10000","DOIUrl":"https://doi.org/10.55251/jmbfs.10000","url":null,"abstract":"Goat cheese and sheep cheese can offer a range of unique flavors and potential health benefits, making them popular choices among cheese lovers. Both types of cheese contain biogenic amines, which are naturally present in many types of food. The purpose of this study was to assess the levels of the seven most commonly occurring biogenic amines in semi-hard and hard goat and sheep cheeses intended for the Slovak market. The HPLC-DAD method with pre-column derivatization using dansylchloride was employed for the analysis of the hard and semi-hard cheese samples. The presence of biogenic amines was confirmed in all cheese samples, with total levels ranging from 14.4 mg.kg-1 to 1322 mg.kg-1. The most abundant compounds found in the samples were tyramine and tryptamine, followed by 2-phenylethylamine and spermine. Histamine was present in 54.5% of the samples, but its concentration did not exceed 68.7 mg.kg-1. Statistically significant differences in the levels of individual biogenic amines were observed between goat and sheep cheeses, except for spermidine and spermine.","PeriodicalId":16348,"journal":{"name":"Journal of microbiology, biotechnology and food sciences","volume":" ","pages":""},"PeriodicalIF":0.9,"publicationDate":"2023-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48538385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Smail Merzougui, Youcef Mahdi, A. Yeddou, B. Nadjemi
Soil properties have an important role in the movement of minerals in the environment. Therefore, the study of mineral adsorption and soil properties can help solve environmental pollution problems. In the present work, batch adsorption of zinc and lead was studied on some Algerian soils (clay soil, sandy loam soil, and sandy soil), as a function of contact time, pH, metal concentration and physicochemical properties. The results showed the metal adsorption amount depends on soil pH and initial ion concentration, soil type, temperature, exchange capacity, and metal hydrolysis. Ionic strength and organic matter content investigation indicate that, clay soil presents greater adsorption of both metals than the two other soils. The metal ions’ adsorption decreases with decreasing pH and increasing ionic strength. The results showed that the adsorption amount of lead is better than that of zinc in all studied samples. The equilibrium data good fit well with Langmuir and Freundlich’s models. The tested kinetic models showed that soil adsorption of these metals are biphasic and diffusion-controlled process. For all pH values, the adsorption was relatively fast at the beginning followed by a slower phase at reaching the equilibrium time.
{"title":"Zn (II) AND Pb (II) ADSORPTION ON TO AGRICULTURAL SOILS FROM EAST OF ALGERIA","authors":"Smail Merzougui, Youcef Mahdi, A. Yeddou, B. Nadjemi","doi":"10.55251/jmbfs.8257","DOIUrl":"https://doi.org/10.55251/jmbfs.8257","url":null,"abstract":"Soil properties have an important role in the movement of minerals in the environment. Therefore, the study of mineral adsorption and soil properties can help solve environmental pollution problems. In the present work, batch adsorption of zinc and lead was studied on some Algerian soils (clay soil, sandy loam soil, and sandy soil), as a function of contact time, pH, metal concentration and physicochemical properties.\u0000The results showed the metal adsorption amount depends on soil pH and initial ion concentration, soil type, temperature, exchange capacity, and metal hydrolysis. Ionic strength and organic matter content investigation indicate that, clay soil presents greater adsorption of both metals than the two other soils. The metal ions’ adsorption decreases with decreasing pH and increasing ionic strength. The results showed that the adsorption amount of lead is better than that of zinc in all studied samples. The equilibrium data good fit well with Langmuir and Freundlich’s models. The tested kinetic models showed that soil adsorption of these metals are biphasic and diffusion-controlled process. For all pH values, the adsorption was relatively fast at the beginning followed by a slower phase at reaching the equilibrium time.","PeriodicalId":16348,"journal":{"name":"Journal of microbiology, biotechnology and food sciences","volume":" ","pages":""},"PeriodicalIF":0.9,"publicationDate":"2023-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41774924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Harini Keerthana Suresh Kumar, Trishala Gopikrishna, T. K, Kumar Perumal, Elavarashi E
Bacillus subtilis Miz-8 is isolated from Bekang, a traditional fermented soybean food of Mizoram, India. Here, we report computationally analyzed strain Miz-8 mapped to the annotated genome of the reference strain Bacillus subtilis subsp. natto BEST195. Miz-8 has a circular chromosome and a small plasmid with genome size of 4105264 bp and 5838 bp, respectively. Genome contains the genes responsible for synthesis of biosynthetic metabolites like surfactin, fengycin, bacillibactin, bacilysin, subtilosin A and alpha amylase production. The strain Miz-8 harbored virulence genes identical to the strain BEST195 render themselves harmless for human consumption. However, Strain Miz-8 has one intact prophage region but no integrase protein, making it incapable of lateral transfer of antimicrobials. Antibiotic resistance genes were predicted among which tmrB gene was on perfect hit. Plasmid of strain Miz-8 contains no prophage sequences and antibiotic resistance genes. Furthermore, there were several single nucleotide polymorphisms and 344 insertion-deletion polymorphisms. Bacillus subtilis Miz-8's genomic information unmasks its functional significance on human health.
{"title":"A COMPUTATIONAL GENOME ANALYSIS OF STRAIN BACILLUS SUBTILIS MIZ-8 ISOLATED FROM BEKANG REVEALS A DISTINCT CHROMOSOME AND PLASMID CONFERRING SELECTIVE ADVANTAGE","authors":"Harini Keerthana Suresh Kumar, Trishala Gopikrishna, T. K, Kumar Perumal, Elavarashi E","doi":"10.55251/jmbfs.9141","DOIUrl":"https://doi.org/10.55251/jmbfs.9141","url":null,"abstract":"Bacillus subtilis Miz-8 is isolated from Bekang, a traditional fermented soybean food of Mizoram, India. Here, we report computationally analyzed strain Miz-8 mapped to the annotated genome of the reference strain Bacillus subtilis subsp. natto BEST195. Miz-8 has a circular chromosome and a small plasmid with genome size of 4105264 bp and 5838 bp, respectively. Genome contains the genes responsible for synthesis of biosynthetic metabolites like surfactin, fengycin, bacillibactin, bacilysin, subtilosin A and alpha amylase production. The strain Miz-8 harbored virulence genes identical to the strain BEST195 render themselves harmless for human consumption. However, Strain Miz-8 has one intact prophage region but no integrase protein, making it incapable of lateral transfer of antimicrobials. Antibiotic resistance genes were predicted among which tmrB gene was on perfect hit. Plasmid of strain Miz-8 contains no prophage sequences and antibiotic resistance genes. Furthermore, there were several single nucleotide polymorphisms and 344 insertion-deletion polymorphisms. Bacillus subtilis Miz-8's genomic information unmasks its functional significance on human health.","PeriodicalId":16348,"journal":{"name":"Journal of microbiology, biotechnology and food sciences","volume":" ","pages":""},"PeriodicalIF":0.9,"publicationDate":"2023-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48383331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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