Zahra Mousavi, Abbas Tanhaeian, Mehdi Mirzaii, Asghar Arab Asadi, Mitra Riasi
This study optimized the expression of the enterocin P peptide (EntP) in CHO cells using medium additions. Furthermore, the minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), and anti-biofilm effects of Ent-P were examined against several gram positive and gram-negative microorganisms. The findings indicated that increasing medium with 0.75 percent glycerol and 0.75 percent DMSO boosted Ent-P peptide synthesis by around two times. According to Ent-P antimicrobial evaluations, Shigella disentri (4 g/mL) and Escherichia coli (128 g/mL) had the lowest and highest MIC values, respectively. In comparison to Enterococcus faecalis and Escherichia coli (3+ to 2+), the Ent-P peptide shown a strong ability to suppress the formation of the biofilm generated by Staphylococcus aureus and Pseudomonas aeruginosa (4 + to +1).
{"title":"OPTIMIZATION OF EXPRESSION AND EVALUATION OF RECOMBINANT ENTEROCIN-P, AS FOOD BIO PRESERVATIVE ON SOME FOOD SPOILAGE BACTERIA","authors":"Zahra Mousavi, Abbas Tanhaeian, Mehdi Mirzaii, Asghar Arab Asadi, Mitra Riasi","doi":"10.55251/jmbfs.6019","DOIUrl":"https://doi.org/10.55251/jmbfs.6019","url":null,"abstract":"This study optimized the expression of the enterocin P peptide (EntP) in CHO cells using medium additions. Furthermore, the minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), and anti-biofilm effects of Ent-P were examined against several gram positive and gram-negative microorganisms. The findings indicated that increasing medium with 0.75 percent glycerol and 0.75 percent DMSO boosted Ent-P peptide synthesis by around two times. According to Ent-P antimicrobial evaluations, Shigella disentri (4 g/mL) and Escherichia coli (128 g/mL) had the lowest and highest MIC values, respectively. In comparison to Enterococcus faecalis and Escherichia coli (3+ to 2+), the Ent-P peptide shown a strong ability to suppress the formation of the biofilm generated by Staphylococcus aureus and Pseudomonas aeruginosa (4 + to +1).","PeriodicalId":16348,"journal":{"name":"Journal of microbiology, biotechnology and food sciences","volume":"30 1","pages":""},"PeriodicalIF":0.9,"publicationDate":"2023-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139342887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Matúš Kučka, K. Ražná, Simona Čerteková, M. Chňapek, Lucia Mikolášová, Z. Gálová, Ž. Balážová
Buckwheat is a pseudocereal from the Polygonaceae family. Two species from this family are commercially exploited – common buckwheat and tartary buckwheat. Buckwheat comes from China, although in recent years, the highest production has been noted in Russia. With its nutritional composition (mainly rutin), it has a beneficial effect on human health. Rutin is mainly contained in the flowers and leaves of buckwheat, and it has antidiabetic, neuroprotective and antioxidant properties; it improves blood pressure and lowers cholesterol levels. In addition to rutin, buckwheat contains bioactive peptides that serve as trypsin inhibitors and have antioxidant and antimicrobial properties. Buckwheat found its use mainly in the field of food and feed production. Amplification polymorphism detection techniques are currently used for the genomic analyses of buckwheat, with 8,884 available markers that include 756 loci. The most frequently used type of molecular markers in buckwheat is the microsatellite markers, which form tandem repeats of short nucleotide motifs. The total number of microsatellites in the tartary buckwheat genome is 37,572, with a frequency of 83.25 microsatellites per 1 Mb. Based on their genetic variability, the buckwheat varieties can be divided into the European and Asian groups, with a lower diversity among the varieties in the European group. Genomic analyses can reveal the genetic relatedness or differences between the individual varieties, as well as losses in genetic purity. The transcriptomic analyses are primarily devoted to the expression of genes responsible for the synthesis of flavonoids, but also those involved in the plant's defense mechanisms, development etc. Molecular analyses revealed that the expression of genes supporting the synthesis of rutin can be favorably influenced by light, darkness, methyl jasmonate, abscisic acid etc. Some buckwheat genes were introduced into Arabidopsis, which subsequently showed improved properties, for example, resistance to drought. These findings not only enhance our understanding of buckwheat at a fundamental level but also hold practical significance for breeding programs focused on enhancing nutritional and agronomic traits in buckwheat varieties.
{"title":"BUCKWHEAT – A GENOMIC, TRANSCRIPTOMIC AND PROTEOMIC VIEW","authors":"Matúš Kučka, K. Ražná, Simona Čerteková, M. Chňapek, Lucia Mikolášová, Z. Gálová, Ž. Balážová","doi":"10.55251/jmbfs.10059","DOIUrl":"https://doi.org/10.55251/jmbfs.10059","url":null,"abstract":"Buckwheat is a pseudocereal from the Polygonaceae family. Two species from this family are commercially exploited – common buckwheat and tartary buckwheat. Buckwheat comes from China, although in recent years, the highest production has been noted in Russia. With its nutritional composition (mainly rutin), it has a beneficial effect on human health. Rutin is mainly contained in the flowers and leaves of buckwheat, and it has antidiabetic, neuroprotective and antioxidant properties; it improves blood pressure and lowers cholesterol levels. In addition to rutin, buckwheat contains bioactive peptides that serve as trypsin inhibitors and have antioxidant and antimicrobial properties. Buckwheat found its use mainly in the field of food and feed production. Amplification polymorphism detection techniques are currently used for the genomic analyses of buckwheat, with 8,884 available markers that include 756 loci. The most frequently used type of molecular markers in buckwheat is the microsatellite markers, which form tandem repeats of short nucleotide motifs. The total number of microsatellites in the tartary buckwheat genome is 37,572, with a frequency of 83.25 microsatellites per 1 Mb. Based on their genetic variability, the buckwheat varieties can be divided into the European and Asian groups, with a lower diversity among the varieties in the European group. Genomic analyses can reveal the genetic relatedness or differences between the individual varieties, as well as losses in genetic purity. The transcriptomic analyses are primarily devoted to the expression of genes responsible for the synthesis of flavonoids, but also those involved in the plant's defense mechanisms, development etc. Molecular analyses revealed that the expression of genes supporting the synthesis of rutin can be favorably influenced by light, darkness, methyl jasmonate, abscisic acid etc. Some buckwheat genes were introduced into Arabidopsis, which subsequently showed improved properties, for example, resistance to drought. These findings not only enhance our understanding of buckwheat at a fundamental level but also hold practical significance for breeding programs focused on enhancing nutritional and agronomic traits in buckwheat varieties.","PeriodicalId":16348,"journal":{"name":"Journal of microbiology, biotechnology and food sciences","volume":"17 1","pages":""},"PeriodicalIF":0.9,"publicationDate":"2023-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139342910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Róbert Nagy, Chaima Neji, Judit Remenyik, Péter Sipos
Recently, sorghum production and utilisation experienced a considerable increase in the food industry due to its outstanding nutritional and functional properties as a gluten-free material. Depending on the genotype, it is a good source of antioxidants and contains a high amount of slowly digestible and resistant starch. This study evaluated the effect of physical (heat) treatment on the functional and rheological properties (water and oil absorption capacities etc.) of commercial sorghum flour. Gluten-free bread were also developed with the use of sorghum as base material and the addition of other gluten-free ingredients and the utilisation of heat treatment. Bread quality and texture profile characteristics (hardness, springiness) were evaluated, and an organoleptic test was also performed. Heat treatment proved to be beneficial in improving some of the functional properties of sorghum flour: it increased water absorption capacity by 20% compared to the control flour. It also improved the development of dough and the texture profile of bread on the baking day but resulted in a rapid quality decrease during storage. Sorghum bread with 10 % fibre content received the highest score during sensory evaluation, and a higher fibre content combined with heat treatment caused a significant decrease in hardness values. In conclusion, physical treatment had a favourable effect on some of the functional properties of sorghum flour and bread, especially water absorption capacity and dough development, and according to the organoleptic evaluation results sorghum bread products can potentially compete with other gluten-free bakery products.
{"title":"EVALUATION OF FUNCTIONAL PROPERTIES OF PHYSICALLY TREATED SORGHUM FLOURS AND DEVELOPMENT OF GLUTEN-FREE SORGHUM BREAD","authors":"Róbert Nagy, Chaima Neji, Judit Remenyik, Péter Sipos","doi":"10.55251/jmbfs.9959","DOIUrl":"https://doi.org/10.55251/jmbfs.9959","url":null,"abstract":"Recently, sorghum production and utilisation experienced a considerable increase in the food industry due to its outstanding nutritional and functional properties as a gluten-free material. Depending on the genotype, it is a good source of antioxidants and contains a high amount of slowly digestible and resistant starch. This study evaluated the effect of physical (heat) treatment on the functional and rheological properties (water and oil absorption capacities etc.) of commercial sorghum flour. Gluten-free bread were also developed with the use of sorghum as base material and the addition of other gluten-free ingredients and the utilisation of heat treatment. Bread quality and texture profile characteristics (hardness, springiness) were evaluated, and an organoleptic test was also performed. Heat treatment proved to be beneficial in improving some of the functional properties of sorghum flour: it increased water absorption capacity by 20% compared to the control flour. It also improved the development of dough and the texture profile of bread on the baking day but resulted in a rapid quality decrease during storage. Sorghum bread with 10 % fibre content received the highest score during sensory evaluation, and a higher fibre content combined with heat treatment caused a significant decrease in hardness values. In conclusion, physical treatment had a favourable effect on some of the functional properties of sorghum flour and bread, especially water absorption capacity and dough development, and according to the organoleptic evaluation results sorghum bread products can potentially compete with other gluten-free bakery products.","PeriodicalId":16348,"journal":{"name":"Journal of microbiology, biotechnology and food sciences","volume":"60 1","pages":""},"PeriodicalIF":0.9,"publicationDate":"2023-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139342837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
K. Habbadi, Faiçal Aoujil, Hiba Yahyaoui, A. Benbouazza, Salma El Iraqui El Houssaini, E. Achbani
Grapevine (Vitis vinifera L.) is one of the most widespread and economically important fruit crops in the world based on its capacity to produce high yields of quality fruit, hectares cultivated and its ability to grow in a wide range of climates and soils. However, it is greatly exposed to a wide variety of pathogens, affecting production and fruit quality. Among the potential threats, Allorhizobium vitis, the causal agent of grapevine crown gall represents a limiting factor in grape production worldwide. It causes vine decline and mortality especially in young vineyards and orchards with important economic losses. Owing of the systemic survival of Allorhizobium vitis in grapevine, copper bactericides and antibiotics are ineffective, and they are able to kill only the bacterium on contact. Therefore, the knowledge of pathogen, effective control and prevention strategies, and sensitive detection methods of pathogen are needed to improve the management of the disease. This review highlights the current state of research and the major acquisitions in this field and provides efficient procedures for isolating from tumors and soil. In addition, this paper discusses the different strategies used for the management of grapevine crown gall along with their drawbacks. Moreover, detection methods for rapid and proper identification of the disease bacteria were provided to enhance the efficiency of control measures and prevent the spread of the pathogen.
{"title":"GRAPEVINE CROWN GALL: CURRENT DATA AND RESEARCH PERSPECTIVES","authors":"K. Habbadi, Faiçal Aoujil, Hiba Yahyaoui, A. Benbouazza, Salma El Iraqui El Houssaini, E. Achbani","doi":"10.55251/jmbfs.10198","DOIUrl":"https://doi.org/10.55251/jmbfs.10198","url":null,"abstract":"Grapevine (Vitis vinifera L.) is one of the most widespread and economically important fruit crops in the world based on its capacity to produce high yields of quality fruit, hectares cultivated and its ability to grow in a wide range of climates and soils. However, it is greatly exposed to a wide variety of pathogens, affecting production and fruit quality. Among the potential threats, Allorhizobium vitis, the causal agent of grapevine crown gall represents a limiting factor in grape production worldwide. It causes vine decline and mortality especially in young vineyards and orchards with important economic losses. Owing of the systemic survival of Allorhizobium vitis in grapevine, copper bactericides and antibiotics are ineffective, and they are able to kill only the bacterium on contact. Therefore, the knowledge of pathogen, effective control and prevention strategies, and sensitive detection methods of pathogen are needed to improve the management of the disease. This review highlights the current state of research and the major acquisitions in this field and provides efficient procedures for isolating from tumors and soil. In addition, this paper discusses the different strategies used for the management of grapevine crown gall along with their drawbacks. Moreover, detection methods for rapid and proper identification of the disease bacteria were provided to enhance the efficiency of control measures and prevent the spread of the pathogen. ","PeriodicalId":16348,"journal":{"name":"Journal of microbiology, biotechnology and food sciences","volume":" ","pages":""},"PeriodicalIF":0.9,"publicationDate":"2023-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42666028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Zafar, Syeda Hafiza Afsheen Zafar, Mehwish Faiz, Zubia Rashid, Natasha Abbas Butt, Afsheen Aman
Exploration of biodiversity led towards the development of commercially important biological metabolites. This study focus on the molecular characterization of dextransucrase biosynthezing lactic acid bacterial strain, L. mesenteroides KIBGE-IB40. Fermentation system was designed to optimize the crucial production parameters of the biosynthesis of dextransucrase by L. mesenteroides. Data acquired from the experimental analysis revealed a significant increase of about 1.69-fold in enzyme titer from 67.13 DSU/ml to 114.04 DSU/ml. The optimum fermentation conditions were found to be fermentation time, 18 hours; fermentation temperature, 25°C; fermentation pH, 7.5. Structural analysis of purified dextran was performed. The morphological analysis of dextran by SEM revealed a porous structure of the polysaccharide. FTIR analysis demonstrates the functional group analysis of the polymer. The significant increase in dextransucrase yield demonstrates the crucial impact of fermentation conditions on the metabolic properties of microbial cells. The obtained results demonstrate a practical approach for the commercial application of L. mesenteroides KIBGE-IB40 dextransucrase in food and other industrial sectors.
对生物多样性的探索有助于开发具有重要商业价值的生物代谢物。本研究的重点是右旋糖苷酶生物合成乳酸菌菌株 L. mesenteroides KIBGE-IB40 的分子特征。研究人员设计了发酵系统，以优化 L. mesenteroides 生物合成葡聚糖酶的关键生产参数。实验分析获得的数据显示，酶滴度从 67.13 DSU/ml 到 114.04 DSU/ml，显著增加了约 1.69 倍。最佳发酵条件为：发酵时间 18 小时；发酵温度 25°C；发酵 pH 值 7.5。对纯化的葡聚糖进行了结构分析。扫描电镜对葡聚糖的形态分析表明，该多糖具有多孔结构。傅立叶变换红外光谱分析显示了聚合物的官能团分析。葡聚糖琥珀酸酶产量的大幅增加表明了发酵条件对微生物细胞代谢特性的重要影响。所获得的结果证明了在食品和其他工业领域商业应用 L. mesenteroides KIBGE-IB40 右旋糖酐酶的实用方法。
{"title":"PRODUCTION AND CHARACTERIZATION OF DEXTRAN BIOSYNTHESIZING GLUCOSYLTRANSFERASE FROM L. MESENETEROIDES KIBGE-IB40","authors":"S. Zafar, Syeda Hafiza Afsheen Zafar, Mehwish Faiz, Zubia Rashid, Natasha Abbas Butt, Afsheen Aman","doi":"10.55251/jmbfs.9562","DOIUrl":"https://doi.org/10.55251/jmbfs.9562","url":null,"abstract":"Exploration of biodiversity led towards the development of commercially important biological metabolites. This study focus on the molecular characterization of dextransucrase biosynthezing lactic acid bacterial strain, L. mesenteroides KIBGE-IB40. Fermentation system was designed to optimize the crucial production parameters of the biosynthesis of dextransucrase by L. mesenteroides. Data acquired from the experimental analysis revealed a significant increase of about 1.69-fold in enzyme titer from 67.13 DSU/ml to 114.04 DSU/ml. The optimum fermentation conditions were found to be fermentation time, 18 hours; fermentation temperature, 25°C; fermentation pH, 7.5. Structural analysis of purified dextran was performed. The morphological analysis of dextran by SEM revealed a porous structure of the polysaccharide. FTIR analysis demonstrates the functional group analysis of the polymer. The significant increase in dextransucrase yield demonstrates the crucial impact of fermentation conditions on the metabolic properties of microbial cells. The obtained results demonstrate a practical approach for the commercial application of L. mesenteroides KIBGE-IB40 dextransucrase in food and other industrial sectors.","PeriodicalId":16348,"journal":{"name":"Journal of microbiology, biotechnology and food sciences","volume":"6 1","pages":""},"PeriodicalIF":0.9,"publicationDate":"2023-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139351426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
V. Zmrhal, A. Svoradová, L. Olexiková, P. Chrenek, J. Vašíček, Petr Slama
The goal of the experiment was to evaluate susceptibility of lymphocytes populations derived from bursa of Fabricius, spleen, thymus, and blood to temperature treatments in vitro. Temperatures used in study was 37 °C (thermoneutral), 41 °C (mild heat stress) and 45 °C (severe heat stress). Cells were incubated for 8 hours and after 3 and 8 hours were measured numbers of apoptotic, necrotic and CellROX positive cells by flow cytometry. Representation of live lymphocyte phenotypes (CD3, CD4, CD8, BU1) in cell populations were also measured. At all temperature treatments, thymus lymphocytes population had the lowest (p < 0.05) percentage of apoptotic cells. On the other hand, significantly highest numbers (p < 0.05) of apoptotic cells were found in bursal lymphocytes after 8 hours of incubation. Differences in numbers of necrotic cells were more pronounced at 45 °C, where after 3 and 8 hours of incubation were significantly highest numbers (p < 0.05) of necrotic cells in spleen. Increasing temperature induce production of reactive oxygen species (ROS) in cells and highest proportion (p < 0.05) of ROS-positive cells were found in bursal and spleen cell population after 8 hours of incubation. Transmission electron microscopy showed loss of mitochondrial matrix and disrupted mitochondrial membrane in mitochondria of lymphocytes at 45 °C as initial damage caused by ROS. From individual cell phenotypes, there were significantly decreased (p < 0.05) B lymphocytes in bursa of Fabricius, CD4+ and CD8+ lymphocytes in thymus and whole T lymphocytes population in spleen at 45 °C. Various susceptibility to temperature can be predictive factor of worse function in some organs in stress conditions where body temperature increase.
{"title":"COMPARISON OF CELL DEATH RATE IN VARIOUS CHICKEN LYMPHOCYTES POPULATIONS IN A RESPONSE TO THREE TEMPERATURE TREATMENTS","authors":"V. Zmrhal, A. Svoradová, L. Olexiková, P. Chrenek, J. Vašíček, Petr Slama","doi":"10.55251/jmbfs.10306","DOIUrl":"https://doi.org/10.55251/jmbfs.10306","url":null,"abstract":"The goal of the experiment was to evaluate susceptibility of lymphocytes populations derived from bursa of Fabricius, spleen, thymus, and blood to temperature treatments in vitro. Temperatures used in study was 37 °C (thermoneutral), 41 °C (mild heat stress) and 45 °C (severe heat stress). Cells were incubated for 8 hours and after 3 and 8 hours were measured numbers of apoptotic, necrotic and CellROX positive cells by flow cytometry. Representation of live lymphocyte phenotypes (CD3, CD4, CD8, BU1) in cell populations were also measured. At all temperature treatments, thymus lymphocytes population had the lowest (p < 0.05) percentage of apoptotic cells. On the other hand, significantly highest numbers (p < 0.05) of apoptotic cells were found in bursal lymphocytes after 8 hours of incubation. Differences in numbers of necrotic cells were more pronounced at 45 °C, where after 3 and 8 hours of incubation were significantly highest numbers (p < 0.05) of necrotic cells in spleen. Increasing temperature induce production of reactive oxygen species (ROS) in cells and highest proportion (p < 0.05) of ROS-positive cells were found in bursal and spleen cell population after 8 hours of incubation. Transmission electron microscopy showed loss of mitochondrial matrix and disrupted mitochondrial membrane in mitochondria of lymphocytes at 45 °C as initial damage caused by ROS. From individual cell phenotypes, there were significantly decreased (p < 0.05) B lymphocytes in bursa of Fabricius, CD4+ and CD8+ lymphocytes in thymus and whole T lymphocytes population in spleen at 45 °C. Various susceptibility to temperature can be predictive factor of worse function in some organs in stress conditions where body temperature increase.","PeriodicalId":16348,"journal":{"name":"Journal of microbiology, biotechnology and food sciences","volume":"81 1","pages":""},"PeriodicalIF":0.9,"publicationDate":"2023-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139351476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
I. Alja’afreh, A. Tarawneh, Yaqeen Al Shbailat, Abla Alzagameem, Husam Al-Marafi, Haitham Albadaineh, T. Rababah, M. Al-U’datt
Medicinal plants have been used for longtime for treatment of many illnesses. Many plants were described in folk heritage to have anti-inflammatory activity to treat digestive diseases. In this study, thirteen plants commonly used by Jordanian folks to treat digestive disorders were investigated to find a connection between their contents and activities and their relationship with their assigned folk use. The ethanolic extracts of the plants were tested for their Total Phenolic Content (TPC), Total Tannin Content (TTC), Total Flavonoid Content (TFC) and their antioxidants activity using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS). In addition to α-Amylase inhibitory activity of the plant extracts was determined. Correlations between TPC, TFC, TTC, protein content with the antioxidant activities (DPPH and ABTS), α-amylase inhibition were determined using Pearson correlation. The phenolic content and flavonoid content showed a high correlation with ABTS activity. The studied plants varied from activation to inhibition of α-Amylase, this may benefit people with hypoglycemia. This diversity of values directs people to which plant to use in case of multi-case disease.
{"title":"ANTIOXIDANT AND Alfa-AMYLASE INHIBITION ACTIVITIES OF MEDICINAL PLANTS ARE TRADITIONALLY USED FOR THE TREATMENT OF DIGESTIVE DISORDERS IN THE NEAR EAST REGION","authors":"I. Alja’afreh, A. Tarawneh, Yaqeen Al Shbailat, Abla Alzagameem, Husam Al-Marafi, Haitham Albadaineh, T. Rababah, M. Al-U’datt","doi":"10.55251/jmbfs.9702","DOIUrl":"https://doi.org/10.55251/jmbfs.9702","url":null,"abstract":"Medicinal plants have been used for longtime for treatment of many illnesses. Many plants were described in folk heritage to have anti-inflammatory activity to treat digestive diseases. In this study, thirteen plants commonly used by Jordanian folks to treat digestive disorders were investigated to find a connection between their contents and activities and their relationship with their assigned folk use. The ethanolic extracts of the plants were tested for their Total Phenolic Content (TPC), Total Tannin Content (TTC), Total Flavonoid Content (TFC) and their antioxidants activity using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS). In addition to α-Amylase inhibitory activity of the plant extracts was determined. Correlations between TPC, TFC, TTC, protein content with the antioxidant activities (DPPH and ABTS), α-amylase inhibition were determined using Pearson correlation. The phenolic content and flavonoid content showed a high correlation with ABTS activity. The studied plants varied from activation to inhibition of α-Amylase, this may benefit people with hypoglycemia. This diversity of values directs people to which plant to use in case of multi-case disease.","PeriodicalId":16348,"journal":{"name":"Journal of microbiology, biotechnology and food sciences","volume":" ","pages":""},"PeriodicalIF":0.9,"publicationDate":"2023-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42407833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Masoud Kazeminia, R. Mahmoudi, S. Mousavi, A. Mehrabi
Milk serves as a vital source of essential nutrients in human diets and must be safe for consumption. In the dairy industry, it is crucial to analyze the physicochemical and microbiological properties of raw cow milk (RCM) throughout the year to gather fundamental information, as milk quality significantly influences the final quality of dairy products. This study aimed to investigate the physicochemical and microbiological properties of 60 RCM samples collected from 15 primary milk collection centers in Qazvin, Iran, and assess the impact of seasonal variation on these properties. The study discovered that the RCM samples contained >7 Log10 cfu/mL of Staphylococcus aureus, total coliform count, aerobic mesophilic plate count, and mold and yeast in 15%, 30%, 37%, and 28% of the samples, respectively. The results indicated that aerobic mesophilic plate count, mold and yeast, total coliform count, and Staphylococcus aureus were higher in warmer seasons compared to colder seasons. Furthermore, the study revealed that the average pH, freezing point, solid-not-fat, and protein content were higher in warm seasons, while the average acidity, lactose, and fat content were higher in cold seasons. The physicochemical and microbiological properties of RCM in the Qazvin region were found to be below the acceptable quality value. Although seasonal variation throughout the year affected the microbial and physicochemical quality of RCM, the differences were not statistically significant (p>0.05), except for the solid-not-fat value in winter (p<0.05).
{"title":"RAW COW MILK QUALITY: PHYSICOCHEMICAL, MICROBIOLOGICAL, AND SEASONAL VARIATION","authors":"Masoud Kazeminia, R. Mahmoudi, S. Mousavi, A. Mehrabi","doi":"10.55251/jmbfs.10078","DOIUrl":"https://doi.org/10.55251/jmbfs.10078","url":null,"abstract":"Milk serves as a vital source of essential nutrients in human diets and must be safe for consumption. In the dairy industry, it is crucial to analyze the physicochemical and microbiological properties of raw cow milk (RCM) throughout the year to gather fundamental information, as milk quality significantly influences the final quality of dairy products. This study aimed to investigate the physicochemical and microbiological properties of 60 RCM samples collected from 15 primary milk collection centers in Qazvin, Iran, and assess the impact of seasonal variation on these properties. The study discovered that the RCM samples contained >7 Log10 cfu/mL of Staphylococcus aureus, total coliform count, aerobic mesophilic plate count, and mold and yeast in 15%, 30%, 37%, and 28% of the samples, respectively. The results indicated that aerobic mesophilic plate count, mold and yeast, total coliform count, and Staphylococcus aureus were higher in warmer seasons compared to colder seasons. Furthermore, the study revealed that the average pH, freezing point, solid-not-fat, and protein content were higher in warm seasons, while the average acidity, lactose, and fat content were higher in cold seasons. The physicochemical and microbiological properties of RCM in the Qazvin region were found to be below the acceptable quality value. Although seasonal variation throughout the year affected the microbial and physicochemical quality of RCM, the differences were not statistically significant (p>0.05), except for the solid-not-fat value in winter (p<0.05).","PeriodicalId":16348,"journal":{"name":"Journal of microbiology, biotechnology and food sciences","volume":" ","pages":""},"PeriodicalIF":0.9,"publicationDate":"2023-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41731531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abdelaziz Hajjaji, Bahia Rached, R. AIT M'HAND, N. Rhallabi, F. Mellouki
The purpose of this study was to evaluate the efficiency of Pseudomonas aeruginosa strain Dokkala (OK383444) isolate against cyst nematode Globodera pallida strain Dokkala (MZ959187) as a biocontrol agent on potato cyst nematode. The test was first conducted in vitro with bacterial suspensions at 105, 106, 107 and 108 CFU/mL against 100 eggs, 100 second-stage juveniles (J2), and 10 cysts of G. pallida and then in vivo on potato variety Desiree infested by cyst nematode in the greenhouse. Four plant growing parameters were evaluated in the treated potato plants by P. aeruginosa in the presence of G. pallida; plant height (cm), tuber weight (kg), wet and dry weight (kg) of the aerial part, and root length (cm). The application of P. aeruginosa in vitro reduced the rate of eggs of G. pallida by 42% and juveniles by 56%. However, the percentages of colonized females with bacteria were not significant (8% and 16% after 96 and 240 h, respectively), whereas normal J2 controls hatched at 100% after 48 h. Furthermore, the in vivo treatment showed an enhancement of 650 g in tuber weight and 19.2 cm in plant length compared to untreated G. Pallida infested plants and 33.34% colonized cysts. This research clearly indicates the ability of P. aeruginosa to reduce the density of potato cyst nematodes and has the potential for the biological control of G. pallida.
{"title":"EFFECT OF THE PSEUDOMONAS AERUGINOSA ON POTATO CYST NEMATODE GLOBODERA PALLIDA","authors":"Abdelaziz Hajjaji, Bahia Rached, R. AIT M'HAND, N. Rhallabi, F. Mellouki","doi":"10.55251/jmbfs.5847","DOIUrl":"https://doi.org/10.55251/jmbfs.5847","url":null,"abstract":"The purpose of this study was to evaluate the efficiency of Pseudomonas aeruginosa strain Dokkala (OK383444) isolate against cyst nematode Globodera pallida strain Dokkala (MZ959187) as a biocontrol agent on potato cyst nematode. The test was first conducted in vitro with bacterial suspensions at 105, 106, 107 and 108 CFU/mL against 100 eggs, 100 second-stage juveniles (J2), and 10 cysts of G. pallida and then in vivo on potato variety Desiree infested by cyst nematode in the greenhouse. Four plant growing parameters were evaluated in the treated potato plants by P. aeruginosa in the presence of G. pallida; plant height (cm), tuber weight (kg), wet and dry weight (kg) of the aerial part, and root length (cm). The application of P. aeruginosa in vitro reduced the rate of eggs of G. pallida by 42% and juveniles by 56%. However, the percentages of colonized females with bacteria were not significant (8% and 16% after 96 and 240 h, respectively), whereas normal J2 controls hatched at 100% after 48 h. Furthermore, the in vivo treatment showed an enhancement of 650 g in tuber weight and 19.2 cm in plant length compared to untreated G. Pallida infested plants and 33.34% colonized cysts. This research clearly indicates the ability of P. aeruginosa to reduce the density of potato cyst nematodes and has the potential for the biological control of G. pallida.","PeriodicalId":16348,"journal":{"name":"Journal of microbiology, biotechnology and food sciences","volume":" ","pages":""},"PeriodicalIF":0.9,"publicationDate":"2023-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44790537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
G. Ramnath, Shathya Jayaraman, R. Thirugnanasampandan, Bhuvaneswari Gunasekaran, Akila Gopalakrishnan
Plant derived essential oils are easily available source for healthcare purpose and it creates increased attention due to undesirable effects of chemically synthesized drugs. Murraya koenigii (L.) Spreng. raw fruit oil was extracted, and its chemical constituents was analyzed. Antioxidant and acetylcholinesterase inhibitory effect was tested under in vitro. MTT assay for evaluating cytotoxic effect on HeLa cells was done. β-phellandrene was identified as major compound in the extracted fruit oil. Scavenging of DPPH radical, inhibition of lipid peroxidation and protection of deoxyribose were found to be excellent. A dose dependent antiacetylcholinesterase activity of oil was observed with IC50 value as 448.12±0.02µg/mL. A significant growth inhibitory effect of oil on cervical cancer cells (HeLa) was measured with an IC50 value of 194.1±0.03µg/mL. Overall, the essential oil extracted from raw fruit of Murraya koenigii (L.) Spreng exhibited several biological properties, and it could be considered for plant-based medicine preparations.
{"title":"CHEMICAL COMPOSITION ANALYSIS AND EVALUATION OF ANTIOXIDANT, ANTIACETYLCHOLINESTERASE AND CYTOTOXICITY OF MURRAYA KOENIGII (L.) SPRENG FRUIT OIL","authors":"G. Ramnath, Shathya Jayaraman, R. Thirugnanasampandan, Bhuvaneswari Gunasekaran, Akila Gopalakrishnan","doi":"10.55251/jmbfs.6135","DOIUrl":"https://doi.org/10.55251/jmbfs.6135","url":null,"abstract":"Plant derived essential oils are easily available source for healthcare purpose and it creates increased attention due to undesirable effects of chemically synthesized drugs. Murraya koenigii (L.) Spreng. raw fruit oil was extracted, and its chemical constituents was analyzed. Antioxidant and acetylcholinesterase inhibitory effect was tested under in vitro. MTT assay for evaluating cytotoxic effect on HeLa cells was done. β-phellandrene was identified as major compound in the extracted fruit oil. Scavenging of DPPH radical, inhibition of lipid peroxidation and protection of deoxyribose were found to be excellent. A dose dependent antiacetylcholinesterase activity of oil was observed with IC50 value as 448.12±0.02µg/mL. A significant growth inhibitory effect of oil on cervical cancer cells (HeLa) was measured with an IC50 value of 194.1±0.03µg/mL. Overall, the essential oil extracted from raw fruit of Murraya koenigii (L.) Spreng exhibited several biological properties, and it could be considered for plant-based medicine preparations.","PeriodicalId":16348,"journal":{"name":"Journal of microbiology, biotechnology and food sciences","volume":" ","pages":""},"PeriodicalIF":0.9,"publicationDate":"2023-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46022532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：