M. Vargová, K. Veszelits Laktičová, František Zigo, I. Rehan
This study objective was to evaluate the hygienic condition of surfaces by microbiological swabs and the effectiveness of disinfectant Virkon S. Level of hygiene was evaluated in a small-capacity slaughterhouse located in the Košice region with a maximum weekly capacity of 5 large livestock units. Microbiological swabs were taken from an area of 10 cm2 before the process of slaughtering, during the process, and after disinfection. Disinfectant Virkon S was used in a 1% concentration during an exposure time of 30 minutes for disinfection of monitored surfaces. Disinfectant was effective on surfaces of cage, wall, floor, and (p<0.0001), where was determined significant decrease of microorganisms, but we recorded 1.0 x 104 colony forming units (CFU)/10 cm2 of the total count of bacteria (TCB) on the cage, 7.5 x 103 CFU/10 cm2 of TCB on the wall and 1.5 x 104 CFU/10 cm2 of TCB on the floor after disinfection, which indicates an insufficient level of disinfection. From the achieved results, it is clear, that the disinfection provided by the disinfectant Virkon S was not effective, as the evaluated disinfectant did not achieve a decrease in the number of microorganisms and thus did not ensure a sufficient level of hygiene. We concluded that it is crucial to effectively disinfect products using the right disinfectant at the right exposure period since it helps to stop the spread of several germs that could contaminate products and have a negative impact on consumers' health.
这项研究的目的是通过微生物拭子评估屠宰场表面的卫生状况以及消毒剂维尔康 S 的效果。卫生水平是在位于科希策地区的一家小规模屠宰场进行评估的，该屠宰场每周最多可屠宰 5 头大型牲畜。在屠宰前、屠宰过程中和消毒后，分别从 10 平方厘米的区域采集微生物拭子。使用浓度为 1%的消毒剂 Virkon S 对监测到的表面进行消毒，消毒时间为 30 分钟。消毒剂对笼子、墙壁和地板表面均有效（P<0.0001），微生物数量显著减少，但我们记录到，消毒后笼子上的细菌总数（TCB）为 1.0 x 104 菌落总数（CFU）/10 平方厘米，墙壁上的细菌总数（TCB）为 7.5 x 103 CFU/10 平方厘米，地板上的细菌总数（TCB）为 1.5 x 104 CFU/10 平方厘米，这表明消毒水平不够。从所取得的结果来看，Virkon S 消毒剂的消毒效果显然不佳，因为所评估的消毒剂并没有减少微生物的数量，因此无法确保足够的卫生水平。我们的结论是，在正确的接触时间内使用正确的消毒剂对产品进行有效消毒至关重要，因为这有助于阻止可能污染产品并对消费者健康产生负面影响的多种病菌的传播。
{"title":"ASSESSMENT OF THE HYGIENIC CONDITION OF THE SLAUGHTERHOUSE BASED ON THE EVALUATION OF MICROBIOLOGICAL SWABS","authors":"M. Vargová, K. Veszelits Laktičová, František Zigo, I. Rehan","doi":"10.55251/jmbfs.9469","DOIUrl":"https://doi.org/10.55251/jmbfs.9469","url":null,"abstract":"This study objective was to evaluate the hygienic condition of surfaces by microbiological swabs and the effectiveness of disinfectant Virkon S. Level of hygiene was evaluated in a small-capacity slaughterhouse located in the Košice region with a maximum weekly capacity of 5 large livestock units. Microbiological swabs were taken from an area of 10 cm2 before the process of slaughtering, during the process, and after disinfection. Disinfectant Virkon S was used in a 1% concentration during an exposure time of 30 minutes for disinfection of monitored surfaces. Disinfectant was effective on surfaces of cage, wall, floor, and (p<0.0001), where was determined significant decrease of microorganisms, but we recorded 1.0 x 104 colony forming units (CFU)/10 cm2 of the total count of bacteria (TCB) on the cage, 7.5 x 103 CFU/10 cm2 of TCB on the wall and 1.5 x 104 CFU/10 cm2 of TCB on the floor after disinfection, which indicates an insufficient level of disinfection. From the achieved results, it is clear, that the disinfection provided by the disinfectant Virkon S was not effective, as the evaluated disinfectant did not achieve a decrease in the number of microorganisms and thus did not ensure a sufficient level of hygiene. We concluded that it is crucial to effectively disinfect products using the right disinfectant at the right exposure period since it helps to stop the spread of several germs that could contaminate products and have a negative impact on consumers' health.","PeriodicalId":16348,"journal":{"name":"Journal of microbiology, biotechnology and food sciences","volume":"140 1","pages":""},"PeriodicalIF":0.9,"publicationDate":"2023-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139318275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Požgajová, M. Kovár, A. Navrátilová, Veronika Fialková, Zuzana Chlebová
Glucose is the primary source of carbon and energy. Cells possess a sophisticated mechanism for sensing glucose and responding to it appropriately. However, hyperglycemia represents the predominant risk factor for the development of diabetes. On the other hand, nutrient starvation, including glucose, may lead to the development of malnutrition. Noteworthy, glucose restriction has been associated with significantly delayed aging. Thus, controlled glucose intake is vital for healthy living. The presented study shows the effect of the oyster mushroom Pleurotus ostreatus ethanolic extract on fission yeast Schizosaccharomyces pombe undergoing glucose-derived stress. Fruiting bodies of P. ostreatus were used for ethanolic extraction and its anti-hyper- and hypo-glycemic stress activity was investigated. Compared were cells treated with ethanolic extract of P. ostreatus (1 or 2%) with untreated control. Different glucose concentrations in growth media were used to analyze its impact on growth intensity, generation time (gt), metabolic activity, ROS generation, MDA content, and cell death and apoptosis of S. pombe cells. Glucose deprivation or its over-supplementation resulted in growth retardation and prolonged cell doubling time (gt) which was significantly adjusted by the oyster mushroom extract. Interestingly, oyster mushroom extract reduced excessive ROS and formed MDA that were produced as a consequence of glycemic stress. Unexpectedly, the extract was able to increase the metabolic activity of cells supplemented with high glucose content and thereby protected them from death. Taken together, our study suggests that growth media supplementation with the ethanolic extract of P. ostreatus protects S. pombe cells from glucose-mediated stress through alleviation of oxidative stress and enhancement of metabolic activity.
葡萄糖是碳和能量的主要来源。细胞具有感知葡萄糖并对其做出适当反应的复杂机制。然而，高血糖是糖尿病发病的主要风险因素。另一方面，营养饥饿（包括葡萄糖）可能导致营养不良。值得注意的是，限制葡萄糖摄入与明显延缓衰老有关。因此，控制葡萄糖摄入量对健康生活至关重要。本研究显示了牡蛎菇（Pleurotus ostreatus）乙醇提取物对承受葡萄糖应激的裂殖酵母（Schizosaccharomyces pombe）的影响。研究人员利用牡蛎菇的子实体进行乙醇提取，并研究了其抗高血糖和低血糖应激的活性。细胞经奥氏刺蒺藜乙醇提取物（1% 或 2%）处理后，与未经处理的对照组进行比较。使用生长培养基中不同浓度的葡萄糖分析其对 S. pombe 细胞的生长强度、生成时间（gt）、代谢活性、ROS 生成、MDA 含量、细胞死亡和凋亡的影响。葡萄糖剥夺或过量补充会导致生长迟缓和细胞倍增时间（gt）延长，而杏鲍菇提取物能显著调节这一点。有趣的是，杏鲍菇提取物减少了因血糖压力而产生的过量 ROS 和 MDA。意想不到的是，杏鲍菇提取物还能提高高糖细胞的代谢活性，从而保护它们免于死亡。综上所述，我们的研究表明，在生长介质中补充奥斯特罗草乙醇提取物可通过减轻氧化应激和提高代谢活性来保护 S. pombe 细胞免受葡萄糖介导的应激。
{"title":"OYSTER MUSHROOM (PLEUROTUS OSTREATUS) ETHANOLIC EXTRACT PROTECTS YEAST CELLS FROM HYPER- AND HYPO-GLYCEMIC STRESS","authors":"M. Požgajová, M. Kovár, A. Navrátilová, Veronika Fialková, Zuzana Chlebová","doi":"10.55251/jmbfs.9817","DOIUrl":"https://doi.org/10.55251/jmbfs.9817","url":null,"abstract":"Glucose is the primary source of carbon and energy. Cells possess a sophisticated mechanism for sensing glucose and responding to it appropriately. However, hyperglycemia represents the predominant risk factor for the development of diabetes. On the other hand, nutrient starvation, including glucose, may lead to the development of malnutrition. Noteworthy, glucose restriction has been associated with significantly delayed aging. Thus, controlled glucose intake is vital for healthy living. The presented study shows the effect of the oyster mushroom Pleurotus ostreatus ethanolic extract on fission yeast Schizosaccharomyces pombe undergoing glucose-derived stress. Fruiting bodies of P. ostreatus were used for ethanolic extraction and its anti-hyper- and hypo-glycemic stress activity was investigated. Compared were cells treated with ethanolic extract of P. ostreatus (1 or 2%) with untreated control. Different glucose concentrations in growth media were used to analyze its impact on growth intensity, generation time (gt), metabolic activity, ROS generation, MDA content, and cell death and apoptosis of S. pombe cells. Glucose deprivation or its over-supplementation resulted in growth retardation and prolonged cell doubling time (gt) which was significantly adjusted by the oyster mushroom extract. Interestingly, oyster mushroom extract reduced excessive ROS and formed MDA that were produced as a consequence of glycemic stress. Unexpectedly, the extract was able to increase the metabolic activity of cells supplemented with high glucose content and thereby protected them from death. Taken together, our study suggests that growth media supplementation with the ethanolic extract of P. ostreatus protects S. pombe cells from glucose-mediated stress through alleviation of oxidative stress and enhancement of metabolic activity.","PeriodicalId":16348,"journal":{"name":"Journal of microbiology, biotechnology and food sciences","volume":"7 1","pages":""},"PeriodicalIF":0.9,"publicationDate":"2023-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139317850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The need for natural antioxidants and phenolics additives is constantly growing, there are a number of aspects that need to be studied before the introduction of technology into production. Therefore, in presented work was studied the antioxidant properties of Stevia products that exist on the European market with comparison them to extracts of in vitro cultures of Stevia plants. The highest antioxidant activity was observed in leaf crops (89%) of in vitro cultures of Stevia rebaudiana which further were selected as components for the development of beverages based on tea, herbs and coffee with improved organoleptic properties. It was estimated that antioxidant activity in different varieties of tea, herbal drinks and coffee was lower compared to the same kinds plus 10% S. rebaudiana leaf culture extracts to be added to beverages. The profile of phenolic compounds in Stevia, tea and coffee consists of a wide range of compounds of phenolic nature, which includes phenolic acids, anthocyanins, flavonoids, catechins but shown different composition. Organoleptic evaluation of beverages (black, green, aronia, hibiscus, chamomile, mint, mate teas, yoga-tea, lapacho, Coffea arabica) and its change under the influence of Stevia additives has been presented.
{"title":"INFLUENCE OF STEVIA PRODUCTS ON BIOCHEMICAL AND ORGANOLEPTIC PROPERTIES OF TEA, COFFEE AND HERBAL DRINKS","authors":"O. Sytar, Diaa Mamdouh, I. Smetanska","doi":"10.55251/jmbfs.6184","DOIUrl":"https://doi.org/10.55251/jmbfs.6184","url":null,"abstract":"The need for natural antioxidants and phenolics additives is constantly growing, there are a number of aspects that need to be studied before the introduction of technology into production. Therefore, in presented work was studied the antioxidant properties of Stevia products that exist on the European market with comparison them to extracts of in vitro cultures of Stevia plants. The highest antioxidant activity was observed in leaf crops (89%) of in vitro cultures of Stevia rebaudiana which further were selected as components for the development of beverages based on tea, herbs and coffee with improved organoleptic properties. It was estimated that antioxidant activity in different varieties of tea, herbal drinks and coffee was lower compared to the same kinds plus 10% S. rebaudiana leaf culture extracts to be added to beverages. The profile of phenolic compounds in Stevia, tea and coffee consists of a wide range of compounds of phenolic nature, which includes phenolic acids, anthocyanins, flavonoids, catechins but shown different composition. Organoleptic evaluation of beverages (black, green, aronia, hibiscus, chamomile, mint, mate teas, yoga-tea, lapacho, Coffea arabica) and its change under the influence of Stevia additives has been presented.","PeriodicalId":16348,"journal":{"name":"Journal of microbiology, biotechnology and food sciences","volume":"1 1","pages":""},"PeriodicalIF":0.9,"publicationDate":"2023-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139321321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Sudirman, Aatikah Dewi Ghaisani, Indah Widiastuti, Herpandi Herpandi, Yohana Novelina Sirat, Miftahul Janna
Diabetes mellitus (DM) is a metabolic disorder that has a significant impact on the quality of life and health. This condition is indicated by high blood glucose or hyperglycemia. Alpha-glucosidase is an important enzyme during carbohydrate digestion to obtain monosaccharides such as glucose. This study aimed to investigate the alpha-glucoside inhibitory activity of polysaccharides from water lettuce (Pistia stratiotes) leaf with different extraction methods. The leaf was extracted by hot water extraction (HWE) and ultrasound-assisted extraction (UAE). The total sugar, crude fiber, and alpha-glucosidase inhibitory activity were analysis by in vitro, then an independent-sample t-test was used to analyze the statistically different. The UAE method showed a high yield of crude extract than the HWE method. However, the total sugar of HWE is higher than UAE, whereas no different of crude fiber level. The extract from the HWE method more effectively inhibits the alpha-glucosidase activity when compared to UAE. Therefore, hot water extraction is the suitable method to extract polysaccharides from a water lettuce (Pistia stratiotes) leaf. This condition also indicated polysaccharides extract potential to develop as an antidiabetes agent.
{"title":"INHIBITION OF ALPHA-GLUCOSIDASE BY POLYSACCHARIDES FROM WATER LETTUCE (Pistia stratiotes) LEAF EXTRACTS WITH DIFFERENT EXTRACTION METHODS","authors":"S. Sudirman, Aatikah Dewi Ghaisani, Indah Widiastuti, Herpandi Herpandi, Yohana Novelina Sirat, Miftahul Janna","doi":"10.55251/jmbfs.9805","DOIUrl":"https://doi.org/10.55251/jmbfs.9805","url":null,"abstract":"Diabetes mellitus (DM) is a metabolic disorder that has a significant impact on the quality of life and health. This condition is indicated by high blood glucose or hyperglycemia. Alpha-glucosidase is an important enzyme during carbohydrate digestion to obtain monosaccharides such as glucose. This study aimed to investigate the alpha-glucoside inhibitory activity of polysaccharides from water lettuce (Pistia stratiotes) leaf with different extraction methods. The leaf was extracted by hot water extraction (HWE) and ultrasound-assisted extraction (UAE). The total sugar, crude fiber, and alpha-glucosidase inhibitory activity were analysis by in vitro, then an independent-sample t-test was used to analyze the statistically different. The UAE method showed a high yield of crude extract than the HWE method. However, the total sugar of HWE is higher than UAE, whereas no different of crude fiber level. The extract from the HWE method more effectively inhibits the alpha-glucosidase activity when compared to UAE. Therefore, hot water extraction is the suitable method to extract polysaccharides from a water lettuce (Pistia stratiotes) leaf. This condition also indicated polysaccharides extract potential to develop as an antidiabetes agent.","PeriodicalId":16348,"journal":{"name":"Journal of microbiology, biotechnology and food sciences","volume":"30 1","pages":""},"PeriodicalIF":0.9,"publicationDate":"2023-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139323775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
K. R. Oluoch, E. Muge, Yvonne Wanjiku Mwangi, Francis Jakim Mulaa
The demand for pectinases in the global market has been on the rise in recent years due to their significant biotechnological uses. The present study involves the characterization of a crude alkaline pectinase from Bacillus halodurans LBW 5117 and the demonstration of its bioscouring potential. The enzyme was an endo polygalacturonase (PGase LBW 5117) that was brown and had a slight fermentation odor. It exhibited an activity of 0.21 U/ml and retained 67, 90.5, 95.2, and 95.2 % of this activity after 1 year of storage at -20, 4, 20, and 30 oC, respectively. Its operational pH and temperature ranged between 10.2 - 11.0 (optimum, 10.5) and 45 - 57 oC (optimum 50 oC), respectively. Metal ions e.g., K+, Ca2+, Mg2+, Fe3+, Cu2+, Na+, Mn2+, and Zn2+ either stimulated or did not significantly affect its activity at 1 mM. The enzyme retained only 15 % of its activity after 8 h of incubation at 50 oC, but this improved to 200 % in the presence of 1.5 mM Ca2+ and 0.05 mM Tween 20. The enzyme was cellulase-free and hydrolyzed pectin in an endo-manner. Moreover, when used under the established optimized operating conditions, it degraded and eliminated pectin (and other non-cellulosic hydrophobic impurities that adhere to it) from woven cotton (weight loss = 0.68 %) and produced a more hydrophilic fabric with improved wettability for water (drop test = 10 sec) and dye (capillary rise test = 28 mm after 30 min). This result shows that PGase LBW 5117 possesses good operational properties, potentially making it a good bioscouring agent. It is, however, recommended that the bioscouring process be optimized, and its effectiveness be compared with that of standard bio- and/or chemical-scouring process(es).
{"title":"CHARACTERIZATION OF AN ALKALINE ENDO-POLYGALACTURONASE (PGase LBW 5117) FROM ALKALIPHILIC BACILLUS HALODURANS LBW 5117 AND DEMONSTRATION OF ITS BIO-SCOURING POTENTIAL","authors":"K. R. Oluoch, E. Muge, Yvonne Wanjiku Mwangi, Francis Jakim Mulaa","doi":"10.55251/jmbfs.9945","DOIUrl":"https://doi.org/10.55251/jmbfs.9945","url":null,"abstract":"The demand for pectinases in the global market has been on the rise in recent years due to their significant biotechnological uses. The present study involves the characterization of a crude alkaline pectinase from Bacillus halodurans LBW 5117 and the demonstration of its bioscouring potential. The enzyme was an endo polygalacturonase (PGase LBW 5117) that was brown and had a slight fermentation odor. It exhibited an activity of 0.21 U/ml and retained 67, 90.5, 95.2, and 95.2 % of this activity after 1 year of storage at -20, 4, 20, and 30 oC, respectively. Its operational pH and temperature ranged between 10.2 - 11.0 (optimum, 10.5) and 45 - 57 oC (optimum 50 oC), respectively. Metal ions e.g., K+, Ca2+, Mg2+, Fe3+, Cu2+, Na+, Mn2+, and Zn2+ either stimulated or did not significantly affect its activity at 1 mM. The enzyme retained only 15 % of its activity after 8 h of incubation at 50 oC, but this improved to 200 % in the presence of 1.5 mM Ca2+ and 0.05 mM Tween 20. The enzyme was cellulase-free and hydrolyzed pectin in an endo-manner. Moreover, when used under the established optimized operating conditions, it degraded and eliminated pectin (and other non-cellulosic hydrophobic impurities that adhere to it) from woven cotton (weight loss = 0.68 %) and produced a more hydrophilic fabric with improved wettability for water (drop test = 10 sec) and dye (capillary rise test = 28 mm after 30 min). This result shows that PGase LBW 5117 possesses good operational properties, potentially making it a good bioscouring agent. It is, however, recommended that the bioscouring process be optimized, and its effectiveness be compared with that of standard bio- and/or chemical-scouring process(es).","PeriodicalId":16348,"journal":{"name":"Journal of microbiology, biotechnology and food sciences","volume":"15 1","pages":""},"PeriodicalIF":0.9,"publicationDate":"2023-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139336394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Beer is an age-old alcoholic, recreational beverage popular worldwide. It is produced by the process of brewing which involves the enzymatic fermentation of the mixture of water, starch, and hops by yeast in a specific condition. Production of beer from cereal grains involved several steps including grain treatment, malting, mashing, lautering, filtration, fermentation, maturation, finishing, and packaging. The brewing process has a marked effect on the quality, taste, aroma, consistency and alcoholic content. Several types of beers with distinct characteristics can be produced by varying the brewing conditions and yeast flora. Apart from psychoactive affects beer can have several medicinal benefits when consumed in moderation. This review presents a detailed understanding of the several steps for enhancing the brewing process for commercial beer production with desirable quality and flavour. The review also highlights current research progress in the innovative methods and techniques adapted for the cost-effective production of beer, including key advances, potential applications and limitations. Therefore, keeping in view the demands of beer in human daily life, there is an extensive need to commercialize these processes by developing and optimizing novel strategies for cost-effective beer production.
{"title":"BEER PRODUCTION BY FERMENTATION PROCESS: A REVIEW","authors":"Archana Goyal, Gaurav Shukla, Shruti Mishra, Sarita Mallik, Anuradha Singh, Manish Dubey","doi":"10.55251/jmbfs.9532","DOIUrl":"https://doi.org/10.55251/jmbfs.9532","url":null,"abstract":"Beer is an age-old alcoholic, recreational beverage popular worldwide. It is produced by the process of brewing which involves the enzymatic fermentation of the mixture of water, starch, and hops by yeast in a specific condition. Production of beer from cereal grains involved several steps including grain treatment, malting, mashing, lautering, filtration, fermentation, maturation, finishing, and packaging. The brewing process has a marked effect on the quality, taste, aroma, consistency and alcoholic content. Several types of beers with distinct characteristics can be produced by varying the brewing conditions and yeast flora. Apart from psychoactive affects beer can have several medicinal benefits when consumed in moderation. This review presents a detailed understanding of the several steps for enhancing the brewing process for commercial beer production with desirable quality and flavour. The review also highlights current research progress in the innovative methods and techniques adapted for the cost-effective production of beer, including key advances, potential applications and limitations. Therefore, keeping in view the demands of beer in human daily life, there is an extensive need to commercialize these processes by developing and optimizing novel strategies for cost-effective beer production.","PeriodicalId":16348,"journal":{"name":"Journal of microbiology, biotechnology and food sciences","volume":"14 1","pages":""},"PeriodicalIF":0.9,"publicationDate":"2023-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139336631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nanoparticles are unique and show different physical and chemical properties from their bulk material. Silver nanoparticles have a variety of antimicrobial applications. In the present study Silver nanoparticles (Ag-NPs) were synthesized by green methodology using the plant Moringa oliefera Lam. leaf extract. The main objective of this study was to synthesize silver nanoparticles without using any hazardous chemicals for future biomedical uses. M. oleifera Lam. is a well-known medicinal plant and is used in the preparation of different ayurvedic medicines. This plant is a rich source of polyphenols, folic acid, and beta-carotene. The effect of temperature on the synthesis of nanoparticles was also determined. The synthesized silver nanoparticles showed an absorbance peak at 430 nm with a UV-Vis spectrophotometer. X-ray diffraction study and TEM analysis exhibited the crystalline nature of silver nanoparticles and revealed that their size is 20-30 nm. An antimicrobial activity study was also performed against the most common nosocomial pathogens and the results revealed that the synthesized Ag-NPs have significant antimicrobial properties against MRSA, Escherichia coli, Klebsiella (ESBL), and Salmonella enterica. The antibacterial efficacy of silver nanoparticles was also determined using MIC and MBC. This study concluded that M. oliefera Lam. exhibited strong potential for the synthesis of silver nanoparticles. These synthesized silver nanoparticles are stable and eco-friendly and could be used to control bacterial growth of common pathogenic microorganisms including some multi-drug resistant (MDR) bacterial strains such as MRSA and Klebsiella (ESBL).
纳米粒子是独特的，其物理和化学性质与块状材料不同。银纳米粒子具有多种抗菌用途。本研究采用绿色方法，利用辣木叶提取物合成了银纳米粒子（Ag-NPs）。本研究的主要目的是在不使用任何有害化学物质的情况下合成银纳米粒子，用于未来的生物医学用途。M. oleifera Lam. 是一种著名的药用植物，可用于制备不同的阿育吠陀药物。这种植物是多酚、叶酸和β-胡萝卜素的丰富来源。还测定了温度对纳米粒子合成的影响。在紫外可见分光光度计上，合成的银纳米粒子在 430 纳米处显示出吸光度峰。X 射线衍射研究和 TEM 分析表明，银纳米粒子具有结晶性质，尺寸为 20-30 纳米。结果表明，合成的 Ag-NPs 对 MRSA、大肠杆菌、克雷伯氏菌（ESBL）和肠炎沙门氏菌具有显著的抗菌特性。此外，还利用 MIC 和 MBC 测定了银纳米粒子的抗菌效果。这项研究认为，M. oliefera Lam.具有合成银纳米粒子的强大潜力。这些合成的银纳米粒子既稳定又环保，可用于控制常见病原微生物的细菌生长，包括一些耐多药（MDR）细菌菌株，如 MRSA 和克雷伯氏菌（ESBL）。
{"title":"DRUMSTICK TREE (Moringa oleifera) Lam. LEAF EXTRACT MEDIATED SYNTHESIS OF SILVER NANOPARTICLES AND THEIR ANTIBACTERIAL ACTIVITY AGAINST NOSOCOMIAL BACTERIAL PATHOGENS","authors":"Nidhi Pal, Shweta Agrawal","doi":"10.55251/jmbfs.6015","DOIUrl":"https://doi.org/10.55251/jmbfs.6015","url":null,"abstract":"Nanoparticles are unique and show different physical and chemical properties from their bulk material. Silver nanoparticles have a variety of antimicrobial applications. In the present study Silver nanoparticles (Ag-NPs) were synthesized by green methodology using the plant Moringa oliefera Lam. leaf extract. The main objective of this study was to synthesize silver nanoparticles without using any hazardous chemicals for future biomedical uses. M. oleifera Lam. is a well-known medicinal plant and is used in the preparation of different ayurvedic medicines. This plant is a rich source of polyphenols, folic acid, and beta-carotene. The effect of temperature on the synthesis of nanoparticles was also determined. The synthesized silver nanoparticles showed an absorbance peak at 430 nm with a UV-Vis spectrophotometer. X-ray diffraction study and TEM analysis exhibited the crystalline nature of silver nanoparticles and revealed that their size is 20-30 nm. An antimicrobial activity study was also performed against the most common nosocomial pathogens and the results revealed that the synthesized Ag-NPs have significant antimicrobial properties against MRSA, Escherichia coli, Klebsiella (ESBL), and Salmonella enterica. The antibacterial efficacy of silver nanoparticles was also determined using MIC and MBC. This study concluded that M. oliefera Lam. exhibited strong potential for the synthesis of silver nanoparticles. These synthesized silver nanoparticles are stable and eco-friendly and could be used to control bacterial growth of common pathogenic microorganisms including some multi-drug resistant (MDR) bacterial strains such as MRSA and Klebsiella (ESBL).","PeriodicalId":16348,"journal":{"name":"Journal of microbiology, biotechnology and food sciences","volume":"30 1","pages":""},"PeriodicalIF":0.9,"publicationDate":"2023-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139336673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Rola Najib, M. Mezher, Tarek Houri, Yara Kairallah, Mahmoud Khalil
Invasion of pathogenic infections, antibiotic resistance, and formation of biofilms represent serious contemporary issues that threaten human health. Quercus cerris L. is a deciduous Mediterranean tree that has been used in folk medicine since the ancient times but without knowing its phytochemical profile. The aim of this study was to evaluate the antibacterial potential of aqueous and ethanolic leaf extracts against two Gram-positive bacteria (Streptococcus intermedius and Enterococcus faecium) and two Gram-negative bacteria (Escherichia coli and Stenotrophomonas maltophilia) using agar well diffusion and broth microdilution methods. The antibiofilm properties of Quercus cerris L. extracts were also tested against the listed bacteria. The results of this study showed that both extracts displayed antibacterial capacity. However, the test cultures were found to be more susceptible to the ethanolic extract than the aqueous one. The lowest minimum inhibitory concentration (MIC) (12 mg/ml) and minimum bactericidal concentration (MBC) (25 mg/ml) values of the ethanolic extract were registered against Escherichia coli, while the highest values were noted against Streptococcus intermedius. As for the aqueous extract, it showed only a bacteriostatic activity against all the tested bacteria with a MIC of 100 mg/ml. The results of the antibiofilm assays also showed that the ethanolic extract exhibited significant antibiofilm effects against the investigated microorganisms. In the light of the findings of this research study, the aqueous and ethanolic extracts of Quercus cerris L. may be very useful in the development of new plant-based antimicrobial agents.
病原体感染、抗生素耐药性和生物膜的形成是当代威胁人类健康的严重问题。柞树（Quercus cerris L.）是一种地中海落叶乔木，自古以来就被用于民间医药，但人们对其植物化学成分并不了解。本研究的目的是采用琼脂井扩散法和肉汤微稀释法，评估水提取物和乙醇叶提取物对两种革兰氏阳性菌（中间链球菌和粪肠球菌）和两种革兰氏阴性菌（大肠杆菌和嗜麦芽血单胞菌）的抗菌潜力。还测试了柞树提取物对所列细菌的抗生物膜特性。研究结果表明，两种提取物都具有抗菌能力。不过，与水提取物相比，测试培养物对乙醇提取物的敏感性更高。乙醇提取物对大肠杆菌的最低抑菌浓度（MIC）（12 毫克/毫升）和最低杀菌浓度（MBC）（25 毫克/毫升）值最低，而对中间链球菌的抑菌浓度（MIC）和杀菌浓度（MBC）值最高。至于水提取物，它只对所有受测细菌显示出抑菌活性，其 MIC 值为 100 毫克/毫升。抗生物膜试验的结果也表明，乙醇提取物对所研究的微生物具有显著的抗生物膜作用。根据这项研究的结果，柞树的水提取物和乙醇提取物可能对开发新的植物抗菌剂非常有用。
{"title":"QUERCUS CERRIS L.: ANTIBACTERIAL AND ANTIBIOFILM POTENTIAL","authors":"Rola Najib, M. Mezher, Tarek Houri, Yara Kairallah, Mahmoud Khalil","doi":"10.55251/jmbfs.9843","DOIUrl":"https://doi.org/10.55251/jmbfs.9843","url":null,"abstract":"Invasion of pathogenic infections, antibiotic resistance, and formation of biofilms represent serious contemporary issues that threaten human health. Quercus cerris L. is a deciduous Mediterranean tree that has been used in folk medicine since the ancient times but without knowing its phytochemical profile. The aim of this study was to evaluate the antibacterial potential of aqueous and ethanolic leaf extracts against two Gram-positive bacteria (Streptococcus intermedius and Enterococcus faecium) and two Gram-negative bacteria (Escherichia coli and Stenotrophomonas maltophilia) using agar well diffusion and broth microdilution methods. The antibiofilm properties of Quercus cerris L. extracts were also tested against the listed bacteria. The results of this study showed that both extracts displayed antibacterial capacity. However, the test cultures were found to be more susceptible to the ethanolic extract than the aqueous one. The lowest minimum inhibitory concentration (MIC) (12 mg/ml) and minimum bactericidal concentration (MBC) (25 mg/ml) values of the ethanolic extract were registered against Escherichia coli, while the highest values were noted against Streptococcus intermedius. As for the aqueous extract, it showed only a bacteriostatic activity against all the tested bacteria with a MIC of 100 mg/ml. The results of the antibiofilm assays also showed that the ethanolic extract exhibited significant antibiofilm effects against the investigated microorganisms. In the light of the findings of this research study, the aqueous and ethanolic extracts of Quercus cerris L. may be very useful in the development of new plant-based antimicrobial agents.","PeriodicalId":16348,"journal":{"name":"Journal of microbiology, biotechnology and food sciences","volume":"7 1","pages":""},"PeriodicalIF":0.9,"publicationDate":"2023-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139336195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Azo-dyes such as crystal violet are commonly used ingredient in all types of dying industries which more often eventually reach water and soil due to washing and disposal practices. They are non-biodegradable inside higher organisms and are less biodegradable due to their xenobiotic character. However, some of the microbes exhibit their extensive potential to degrade such recalcitrants. This study reports the isolation and characterization of a newly isolated Klebsiella sp. strain RGUDBI01 from petroleum-contaminated soil samples. The species-level identification was done by 16S rDNA gene sequencing (ON945611.1). The isolate exhibited 88% azo-dye degradation just after 3 days of incubation under optimized conditions. The treated samples exhibited a significant increase in the growth of Cicer arietinum seeds compared to the control group. This result suggests that the dye-contaminated samples, after treatment, displayed non-cytotoxic behavior. The strain also showed its potential to tolerate and withstand a wide range of pH, salinity, and substrate concentration which supports its futuristic environmental application. In addition to the above, the strain could also produce biosurfactants with ʋC=C, ʋC=C-H, and ʋC-C functional groups which were evaluated based on FTIR spectral analysis.
{"title":"CHARACTERIZATION OF A NEWLY ISOLATED AZO-DYE DEGRADING KLEBSIELLA SP. STRAIN RGUDBI01","authors":"Sahiba Khan, Indukalpa Das, Prapti Baishya, Madhumita Roy, Susmita Das, Debasish Borbora, Debajit Borah","doi":"10.55251/jmbfs.10275","DOIUrl":"https://doi.org/10.55251/jmbfs.10275","url":null,"abstract":"Azo-dyes such as crystal violet are commonly used ingredient in all types of dying industries which more often eventually reach water and soil due to washing and disposal practices. They are non-biodegradable inside higher organisms and are less biodegradable due to their xenobiotic character. However, some of the microbes exhibit their extensive potential to degrade such recalcitrants. This study reports the isolation and characterization of a newly isolated Klebsiella sp. strain RGUDBI01 from petroleum-contaminated soil samples. The species-level identification was done by 16S rDNA gene sequencing (ON945611.1). The isolate exhibited 88% azo-dye degradation just after 3 days of incubation under optimized conditions. The treated samples exhibited a significant increase in the growth of Cicer arietinum seeds compared to the control group. This result suggests that the dye-contaminated samples, after treatment, displayed non-cytotoxic behavior. The strain also showed its potential to tolerate and withstand a wide range of pH, salinity, and substrate concentration which supports its futuristic environmental application. In addition to the above, the strain could also produce biosurfactants with ʋC=C, ʋC=C-H, and ʋC-C functional groups which were evaluated based on FTIR spectral analysis.","PeriodicalId":16348,"journal":{"name":"Journal of microbiology, biotechnology and food sciences","volume":"36 1","pages":""},"PeriodicalIF":0.9,"publicationDate":"2023-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139337038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
P. Haščík, A. Pavelkova, M. Čech, Lukáš Jurčaga, Andrea Mesárošová, M. Fik
The aim of the work was to evaluate the colour and basic chemical composition (% of water, protein, fat and cholesterol) of the muscles: back (m. longissimus dorsi - MLD), shoulder (m. deltoideus - MD) and thigh (m. semimembranosus - MSM) (n=12) of the European badger. The L* values of the European badger ranged from 31.02 (MLD) to 36.81 (MSM) and significant differences (P≤0.05) were observed between the MSM and MD, respectively MLD. The a* value ranged from 12.60 (MD) to 13.80 (MSM). By evaluating the b* parameter, we observed a similar tendency as with the L* value. The highest values were measured in the MSM (11.17) and lower in the MD (8.58), respectively MLD (8.10). The water content varied from 73.13% (MLD) to 74.11% (MD) with no significant differences between the monitored muscles of the European badger (P≥0.05). The average protein content of MD, MLD and MSM was 22.67 g.100 g-1, whereas the highest content of protein was recorded in MLD (22.77 g.100 g-1) (P≥0.05). The highest fat content was measured in MSM (2.06 g.100 g-1) and the lowest (0.95 g.100 g-1) in MD. We observed significant differences (P≤0.05) in fat content of MLD and MD compared to MSM. The cholesterol content in meat from wild European badgers hunted in Slovakia ranged from 0.52 g.100 g-1 (MD) to 0.61 g.100 g-1 (MSM). The significant differences (P≤0.05) were between the MSM and MD.
{"title":"PHYSICOCHEMICAL EVALUATION OF BADGER (MELES MELES) MEAT","authors":"P. Haščík, A. Pavelkova, M. Čech, Lukáš Jurčaga, Andrea Mesárošová, M. Fik","doi":"10.55251/jmbfs.10423","DOIUrl":"https://doi.org/10.55251/jmbfs.10423","url":null,"abstract":"The aim of the work was to evaluate the colour and basic chemical composition (% of water, protein, fat and cholesterol) of the muscles: back (m. longissimus dorsi - MLD), shoulder (m. deltoideus - MD) and thigh (m. semimembranosus - MSM) (n=12) of the European badger. The L* values of the European badger ranged from 31.02 (MLD) to 36.81 (MSM) and significant differences (P≤0.05) were observed between the MSM and MD, respectively MLD. The a* value ranged from 12.60 (MD) to 13.80 (MSM). By evaluating the b* parameter, we observed a similar tendency as with the L* value. The highest values were measured in the MSM (11.17) and lower in the MD (8.58), respectively MLD (8.10). The water content varied from 73.13% (MLD) to 74.11% (MD) with no significant differences between the monitored muscles of the European badger (P≥0.05). The average protein content of MD, MLD and MSM was 22.67 g.100 g-1, whereas the highest content of protein was recorded in MLD (22.77 g.100 g-1) (P≥0.05). The highest fat content was measured in MSM (2.06 g.100 g-1) and the lowest (0.95 g.100 g-1) in MD. We observed significant differences (P≤0.05) in fat content of MLD and MD compared to MSM. The cholesterol content in meat from wild European badgers hunted in Slovakia ranged from 0.52 g.100 g-1 (MD) to 0.61 g.100 g-1 (MSM). The significant differences (P≤0.05) were between the MSM and MD.","PeriodicalId":16348,"journal":{"name":"Journal of microbiology, biotechnology and food sciences","volume":"26 1","pages":""},"PeriodicalIF":0.9,"publicationDate":"2023-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139341942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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