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Digit Ratio (2D:4D) and Performance of Chinese Elite Archers 中国优秀弓箭手的数字比(2D:4D)与成绩
IF 0.7 4区 医学 Q4 Medicine Pub Date : 2022-11-14 DOI: 10.31083/j.jomh1811214
Jin Wu, Xinlong Liu, Yujia Wang, Yunlu Zhang, Yongzhao Fan
Background : Previous studies have found a correlation between digit ratio (2D:4D) and performance in sporting events which require physical competence. Such correlation, however, has not been well studied in technique-oriented sports. This study explored the correlation between 2D:4D and sporting performance of Chinese elite archers. Also it provides some references for sports research in other fields. Methods : We conducted the study with 31 Chinese elite archers (15 males and 16 females) preparing for the 2020 Tokyo Olympic Games and collected their 2D:4D ratios by measuring their index and ring fingers. The study also asked them to take physical fitness tests including push-ups, decline sit-ups, half-squat and a 4000-meter run, and obtained their results in former ranking tournaments and Olympic elimination series. The study recorded every entry of data in a “mean ± standard deviation” (M ± SD) format and processed them in SPSS 24.0 to produce One-Way ANOVA and Pearson correlations. Results : Our study found no significant difference between varied sporting levels in the left 2D:4D, and between world-class and national-level archers in the right 2D:4D ( p > 0.05), yet a significant one between left-right 2D:4D of world-class, national-level, and level-1 archers ( p < 0.01). We also found no significant correlation between the left 2D:4D and specific physical fitness test results, and between those archers’ results. Similarly, there was a significant negative correlation between the right 2D:4D and specific test results ( p < 0.05), and a strong negative correlation between that and results of ranking tournaments ( p < 0.01) and Olympic elimination series ( p < 0.01). Conclusions : Chinese elite archers with lower right 2D:4D are of better physical fitness and enhanced sport performance. Hence, the right 2D:4D may act as a critical indicator in selecting excellent archers.
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引用次数: 0
An Examination of Longitudinal Changes in Korean Middle School Students’ Psychological Health According to Their Body Mass Index and Weekly Exercise Hours 从体重指数和每周运动时数看韩国中学生心理健康的纵向变化
IF 0.7 4区 医学 Q4 Medicine Pub Date : 2022-11-11 DOI: 10.31083/j.jomh1811212
Eun-sung Kim, H. Ahn, Minhye Shin, Young-ho So
Background : It is necessary for adolescents to maintain proper body mass index (BMI) and regular exercise for proper growth, development, and psychological health. Existing studies have demonstrated the significant positive effect of regular physical activity or exercise on preventing socio-psychological problems and cognitive body image issues in adolescents. This study aimed to investigate the longitudinal changes in psychological health factors of mental health, self-concept, self-esteem, and self-efficacy according to BMI and weekly exercise hours among Korean middle school students. Methods : Cohort data were obtained from the Gyeonggi Institute of Education for three years (2015–2017), and a total of 16,027 data points were analyzed. We performed a repeated-measures analysis of variance (3 grades × 4 body mass index groups × 5 weekly exercise hours groups) using SPSS software (version 21.0, IBM Corp., Armonk, NY, USA) for data analysis. Results : The results obtained through the analysis are as follows. As school grades rose among Korean middle school students, psychological health factors such as mental health, self-concept, self-esteem, and self-efficacy decreased. And the deterioration of psychological health factors showed lower positive results for normal and underweight students than for over-weight and obese students. Students with a lot of exercise time every week showed a lower decrease in psychological health factors. Conclusions : The results suggest that education for health promotion, considering body mass index and daytime exercise, is necessary to prevent the deterioration of psychological health factors in Korean middle school students.
背景:青少年有必要保持适当的体重指数(BMI)和定期锻炼,以促进适当的生长、发育和心理健康。现有研究表明,有规律的体育活动或锻炼对预防青少年的社会心理问题和认知身体形象问题具有显著的积极作用。本研究旨在根据BMI和每周锻炼时间,调查韩国中学生心理健康、自我概念、自尊和自我效能等心理健康因素的纵向变化。方法:从京畿道教育研究院获得三年(2015-2017)的队列数据,共分析16027个数据点。我们使用SPSS软件(21.0版,IBM Corp.,Armonk,NY,USA)进行了重复测量方差分析(3个等级×4个体重指数组×5个每周运动时间组),用于数据分析。结果:通过分析得到的结果如下。随着韩国中学生学业成绩的提高,心理健康因素如心理健康、自我概念、自尊和自我效能感下降。心理健康因素的恶化显示,正常和体重不足的学生的阳性结果低于超重和肥胖的学生。每周有大量运动时间的学生心理健康因素的下降幅度较小。结论:为防止韩国中学生心理健康因素的恶化,有必要进行健康促进教育,同时考虑体重指数和日间锻炼。
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引用次数: 1
Health Perceptions of Korean and Japanese Adolescents During the Prolonged COVID-19 Pandemic: An Importance Performance Analysis 韩国和日本青少年在COVID-19大流行期间的健康观念:一项重要绩效分析
IF 0.7 4区 医学 Q4 Medicine Pub Date : 2022-11-11 DOI: 10.31083/j.jomh1811211
Hyun-su Youn, Eui-jae Lee, Sukou Riki, Chizuru Mizno, Chul-Min Kim, Yoshinori Okade
Background: This study compares and analyzes the importance and performance of Korean and Japanese adolescents' health awareness in the long COVID-19 pandemic situation. Methods: A frequency analysis was conducted on data collected from 1341 Korean and Japanese adolescents in September 2021 through online and offline surveys to confirm their characteristics (reliability was verified through Cronbach's alpha). A paired sample test was conducted to analyze health awareness differences and performance of each variable between Korean and Japanese middle-school students and between male and female participants, substantiated by importance-performance analysis (IPA). Results: First, Korean adolescents perceived importance for all factors of health perception greater as compared to their Japanese counterparts. Second, performance differences between Korean and Japanese adolescents were especially significant in hygiene management, disease management and physical activity. Third, in Quadrant 4 of the IPA matrix, there were similarities and differences in a particular factor of health perception between Korean and Japanese adolescents. On this basis, we proposed measures emphasizing the importance of health, to enhance Korean and Japanese adolescents' performance. Conclusions: It is important for national government, public education institutions, and families to couple a therapeutic approach with a preventive and management approach that encourages periodic exercise, desirable diet, and adequate sleep when exploring measures to maintain and promote adolescents' health.
背景:本研究对比分析韩国和日本青少年健康意识在长期COVID-19大流行形势下的重要性和表现。方法:对2021年9月收集的1341名韩国和日本青少年的在线和离线调查数据进行频率分析,以确定其特征(通过Cronbach's alpha验证信度)。本研究采用配对样本检验,分析了韩国和日本中学生、男女被试之间健康意识差异和各变量的表现,并采用重要性-表现分析(IPA)加以证实。结果:第一,与日本青少年相比,韩国青少年对健康感知的所有因素的重要性都更高。第二,韩国和日本青少年在卫生管理、疾病管理和体育活动方面的表现差异尤其显著。第三,在IPA矩阵的象限4中,韩国和日本青少年在健康感知的特定因素上存在相似和差异。在此基础上,我们提出了强调健康重要性的措施,以提高韩国和日本青少年的成绩。结论:在探索维持和促进青少年健康的措施时,国家政府、公共教育机构和家庭应将治疗方法与预防和管理方法结合起来,鼓励定期锻炼、合理饮食和充足睡眠。
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引用次数: 0
A Comparative Study on the Effect of Self-Determined Motivation of Generation Z on Their Exercise Adherence Intention According to Their Satisfaction with Body Image and Gender Z世代自我决定动机对身体形象满意度和性别满意度运动坚持意愿影响的比较研究
IF 0.7 4区 医学 Q4 Medicine Pub Date : 2022-11-11 DOI: 10.31083/j.jomh1811213
In-Ock Lee, Chulhwan Choi, Chul-Ho Bum
Background : Generation Z is accustomed to using social media to expose themselves to others. Due to the characteristics of this generation, the intention to continue exercise differs depending on the satisfaction with one’s body image. Methods : This study analyzed (a) the effect of Generation Z’s self-determined motivation to exercise on their exercise adherence intention and (b) the moderating effect of satisfaction with body image and gender between self-determined motivation and exercise adherence intention. Data from 451 Generation Z (born during 1995–2010) university students in Republic of Korea were analyzed on self-determined motivation and exercise adherence intention by structural equation modeling (SEM). Results : The effect of self-determined motivation on exercise adherence intention showed that external regulation had a significant negative effect on exercise adherence intention. In contrast, identified regulation and intrinsic motivation had a significant positive effect on exercise adherence intention. Moreover, this study compared the differences between the four classified groups according to the gap between perceived and ideal body images, level of satisfaction or dissatisfaction with the body image, and gender. The comparison results indicated that external regulation had a significant negative effect on exercise adherence intention in all groups. In the group of men who were satisfied with their body image, intrinsic motivation had a positive effect on their exercise adherence intention. Conclusions : Based on the analysis results, Generation Z’s exercise adherence intention was more affected by internal satisfaction rather than external motivations. It is significance lies in the fact that it focused on Generation Z, who will be the future leading consumers.
背景:Z世代习惯于利用社交媒体向他人展示自己。由于这一代人的特点,继续锻炼的意愿因对身体形象的满意度而异。方法:本研究分析了Z世代自主运动动机对其运动坚持意愿的影响,以及对身体形象和性别的满意度对自主运动动机和运动坚持意愿之间的调节作用。采用结构方程模型(SEM)对韩国451名Z世代(1995-2010年出生)大学生的自主动机和运动坚持意愿进行了分析。结果:自主动机对运动坚持意向的影响表明,外部调节对运动坚持意愿有显著的负向影响。相反,确定的调节和内在动机对运动坚持意愿有显著的积极影响。此外,本研究根据感知和理想身体形象之间的差距、对身体形象的满意或不满意程度以及性别,比较了四个分类组之间的差异。比较结果表明,在所有组中,外部调节对运动坚持意愿都有显著的负面影响。在对自己的身体形象感到满意的男性组中,内在动机对他们的运动坚持意愿有积极影响。结论:根据分析结果,Z世代的运动坚持意愿更多地受到内部满意度的影响,而不是外部动机的影响。它的意义在于它关注的是Z世代,他们将是未来的领先消费者。
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引用次数: 1
An Analysis of Difference in Preventive Health Behaviors for COVID-19 by Personality Characteristics in College Athletes 大学生运动员人格特征对新冠肺炎预防健康行为的差异分析
IF 0.7 4区 医学 Q4 Medicine Pub Date : 2022-11-01 DOI: 10.31083/j.jomh1811210
Yeon Kim, Youngjae Kim, Jeong-hyung Cho
Background: Dueto the uniqueness of COVID-19 compared with other infectious diseases, the need for various countermeasures against it should be emphasized, and considering that most athletes are not allowed to wear masks during competitions, stricter preventive health behaviors for COVID-19 are required among these individuals. Therefore, in an attempt to yield data that may be used to increase the engagement in preventive health behaviors for COVID-19 among college athletes, this study investigated the differences in preventive health behaviors for COVID-19 according to personality types. Material and Methods: Based on the Big Five personality theory, the relationship between preventive health behaviors for COVID-19 and personality characteristics was tested. Data were collected from 65 participants (male: n = 53, female: n = 12) through a questionnaire survey. Results: The results showed no differences in preventive health behaviors according to demographic characteristics such as sex, grade, sports type, and career. The highest score for a personality type was for conscientiousness, and those with a higher level of conscientiousness, extraversion, neuroticism, and openness reported higher scores for preventive health behavior for COVID-19. Interestingly, those with low openness also reported higher scores for preventive health behaviors for COVID-19. Conclusions: These results are expected to be used as important information to restrict the spread of COVID-19 and promote preventive health behaviors among college athletes.
背景:由于COVID-19与其他传染病相比具有独特性,需要强调各种应对措施的必要性,并且考虑到大多数运动员在比赛中不允许戴口罩,因此需要对这些个体进行更严格的COVID-19预防健康行为。因此,为了获得可用于提高大学生运动员COVID-19预防健康行为参与度的数据,本研究根据人格类型调查了COVID-19预防健康行为的差异。材料与方法:基于大五人格理论,对新冠肺炎预防健康行为与人格特征的关系进行检验。通过问卷调查的方式收集了65名参与者(男性53人,女性12人)的数据。结果:预防健康行为在性别、年级、运动类型、职业等人口统计学特征上无显著差异。一种人格类型的最高得分是尽责性,而那些尽责性、外向性、神经质和开放性水平较高的人在COVID-19的预防性健康行为方面得分较高。有趣的是,那些开放度低的人在COVID-19的预防性健康行为方面也报告了更高的分数。结论:本研究结果可为限制新冠病毒在高校运动员中的传播、促进预防健康行为提供重要信息。
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引用次数: 0
Comparative Analysis of Exercise Intensity in Taekkyeon Training Movements 跆拳道训练动作中运动强度的对比分析
IF 0.7 4区 医学 Q4 Medicine Pub Date : 2022-10-26 DOI: 10.31083/j.jomh1810209
Suzuki Jin, Wilson. So, Dong‐il Seo
Background : Taekkyeon is designated as Korea’s Important Intangible Cultural Property No. 76 and listed as a UNESCO intangible cultural heritage. This study aimed to analyze the exercise intensity of Taekkyeon, with a focus on Honjayikhigi (“the movements for practice on their own while moving or in place; the basic movements of Taekkyeon”), Bondaeboegi (“the method of practicing movements that harmoniously connect Taekkyeon’s offense and defense movements”), and Majumegigi (“the method of practicing hand and kick movements for offense”) training movements, which are components of Taekkyeon. Methods : Eleven Taekkyeon instructors were included in this study. The performance of Taekkyeon movements was assessed at Dongguk University in March 2022. During Honjayikhigi, Bondaeboegi, and Majumegigi training movements, heart rates were measured using a polar monitor. For all variables, the means and standard deviations were calculated using descriptive statistics, and a paired sample t -test was used to compare the heart rates among Honjayikhigi, Bondaeboegi, and Majumegigi training movements. Statistical significance was set at α = 0.05. Results : The average heart rates during Honjayikhigi, Bondaeboegi, and Majumegigi training movements were 131.42 ± 8.85, 128.53 ± 8.43, and 126.34 ± 9.26 beats/min, respectively. The exercise intensity of Honjayikhigi was significantly higher than that of Majumegigi ( p = 0.200). In Honjayikhigi, the exercise intensities of Naagamyoegigi ( p = 0.007) and Mannajungikhigi ( p = 0.017) were significantly higher than that of Seoseoikhigi. In Bondaeboegi, the exercise intensities of Apaugery Yeodulmadang and Diaugery Nemadang were not significantly different. In Majumegigi, the exercise intensities of Ikhimsu ( p < 0.001) and Majuchagi ( p < 0.001) were higher than that of Majugeori, whereas those of Ikhimsu ( p = 0.003) and Majuchagi ( p < 0.001) were higher than that of Maguemgil. The exercise intensities of Ikhimsu ( p = 0.001) and Majuchagi ( p < 0.001) were higher than that of Allermegigi. Conclusions : Adjusting the training sequence and duration according to Taekkyeon level must be considered when establishing a training program because of the apparent differences in exercise intensity among the training movements. Our findings suggest that a greater training efficacy can be achieved if training levels in each Taekkyeon movement are properly classified and customized for beginners.
背景:跆拳道被指定为韩国重要非物质文化遗产第76号,被列入联合国教科文组织非物质文化遗产名录。本研究旨在分析跆拳道的运动强度,重点是跆拳道的组成部分“Honjayikhigi”(“在移动或原地进行自己练习的动作;跆拳道的基本动作”),“Bondaeboegi”(“将跆拳道的进攻和防守动作和谐地联系起来的练习动作的方法”)和“Majumegigi”(“练习进攻的手和脚动作的方法”)训练动作。方法:以11名跆拳道教练为研究对象。2022年3月在东国大学对跆拳道动作进行了评价。在“Honjayikhigi”、“Bondaeboegi”和“Majumegigi”训练运动中,使用极地监测器测量心率。对于所有变量,使用描述性统计计算均值和标准差,并使用配对样本t检验比较Honjayikhigi, Bondaeboegi和Majumegigi训练动作的心率。统计学意义设为α = 0.05。结果:Honjayikhigi、Bondaeboegi和Majumegigi训练动作时的平均心率分别为131.42±8.85、128.53±8.43和126.34±9.26次/分。Honjayikhigi的运动强度显著高于Majumegigi (p = 0.200)。在Honjayikhigi中,Naagamyoegigi (p = 0.007)和Mannajungikhigi (p = 0.017)的运动强度显著高于Seoseoikhigi。在Bondaeboegi, Apaugery yeduulmadang和Diaugery Nemadang的运动强度无显著差异。在Majumegigi中,Ikhimsu (p < 0.001)和Majuchagi (p < 0.001)的运动强度高于majugei,而Ikhimsu (p = 0.003)和Majuchagi (p < 0.001)的运动强度高于Maguemgil。Ikhimsu (p = 0.001)和Majuchagi (p < 0.001)的运动强度高于Allermegigi (p < 0.001)。结论:由于各训练动作的运动强度存在明显差异,在制定训练计划时应考虑根据跆拳道水平调整训练顺序和时间。我们的研究结果表明,如果对每个跆拳道动作的训练水平进行适当的分类和定制,可以达到更大的训练效果。
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引用次数: 0
Degendering Male Nursing Students’ Intimate Care Provision: A South African Perspective 非性别化男护生亲密照护的提供:一个南非的视角
IF 0.7 4区 医学 Q4 Medicine Pub Date : 2022-10-26 DOI: 10.31083/j.jomh1810208
S. Shakwane
Background : Male nursing students remain a minority group in nursing education and training programmes. They are thought to have diverted from their prescribed roles as men, leading to professional stereotypes and fear of providing intimate care. In addition, the lack of role models and support for male nurses adds to their frustration during clinical placement. The aim of this study was thus to understand male nursing students’ experiences when providing intimate care to diverse patients during clinical placement. Methods : A descriptive phenomenology design was employed. Male nursing students from two nursing education institutions in Gauteng province, South Africa, were purposively sampled based on their experiences and their exposure to providing basic nursing care requiring physical closeness and touch. Twelve (12) male nursing students participated in individual semi-structured interviews. Data were analysed using phenomenological analysis of horizontalisation, creating meaning and essence of intimate care experiences. Results : Four themes emerged from intimate care experiences, which were (1) helping others with basic physical care; (2) cultural issues related to touching naked bodies of diverse patients; (3) adherence to basic nursing care principles; and (4) discomforting reactions during intimate care and touch. Conclusions : Male nursing students are willing to provide quality care to diverse patients. However, some do not accept intimate care and touch based on gender. For male nursing students to be competent in caring for patients, they must be able to provide intimate care to diverse patients confidently and comfortably. Thus, degendering intimate care provision is essential for male nursing students—to provide care without fear of being stereotyped and misinterpreted as sexual predators. Instead, they must be accepted as nurses who are helping patients with their physical needs.
背景:在护理教育和培训项目中,男护理专业学生仍然是少数群体。他们被认为偏离了规定的男性角色,导致了职业刻板印象和对提供亲密护理的恐惧。此外,缺乏对男护士的榜样和支持,增加了他们在临床实习期间的挫败感。因此,本研究的目的是了解男性护理专业学生在临床实习期间为不同患者提供亲密护理的经历。方法:采用描述性现象学设计。来自南非豪登省两所护理教育机构的男护理学生根据他们的经历和提供需要身体亲密和触摸的基本护理的接触情况进行了有针对性的抽样。十二(12)名男护生参加了个人半结构化访谈。数据分析采用现象学分析的水平化,创造意义和本质的亲密护理经验。结果:亲密护理经历产生了四个主题,分别是:(1)帮助他人进行基本的身体护理;(2)与触摸不同患者裸体有关的文化问题;(3)遵守基本护理原则;(4)亲密护理和触摸过程中的不适反应。结论:男护生愿意为不同类型的患者提供优质护理。然而,有些人不接受基于性别的亲密照顾和触摸。男护生要想有能力照顾病人,就必须能够自信舒适地为不同的病人提供亲密的护理。因此,对男性护理专业的学生来说,提供降级的亲密护理至关重要——提供护理时不必担心被定型和误解为性侵犯者。相反,他们必须被接纳为护士,帮助患者满足他们的身体需求。
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引用次数: 0
Green Environment, Mental Health, and Loyalty among Male and Female Patients 绿色环境、心理健康与男女患者忠诚度的关系
IF 0.7 4区 医学 Q4 Medicine Pub Date : 2022-10-25 DOI: 10.31083/j.jomh1810207
E. Untaru, Antonio Ariza-Montes, Hyeran Kim, Heesup Han
Background : Existing studies revealed that exposure to green spaces within healthcare establishments has multiple physical and mental health benefits to patients. In this context, the concept of biophilic design has received growing attention among environmental psychology researchers. Several studies indicated that the positive effect of green environment may be different for males and females. Therefore, the present study sought to investigate the influence of biophilic design elements (i.e., green items and natural light) on patients’self-rated mental health value, satisfaction with medical care, and loyalty toward the healthcare establishment. The study also investigated the possible influence of gender differences in the relationships between the variables. Methods : A structural equation modeling was employed as a data analysis technique. Results : Our empirical result indicated that biophilic design elements significantly improved the patients’ self-rated mental health value, and this dimension had a positive effect on their satisfaction with medical care and loyalty toward the health care facility. Our findings indicated that the relationships among biophilic design elements, self-rated mental health value, satisfaction with medical care, and loyalty toward the healthcare establishment were substantially different across male and female groups. Moreover, self-rated mental health value and satisfaction with medical care acted as significant mediators between bio-philic design elements and loyalty. Conclusions : Results of this study offer healthcare practitioners and researchers valuable strategies to effectively incorporate biophilic design elements into the interior spaces of a healthcare establishment.
背景:现有研究表明,接触医疗机构内的绿色空间对患者的身心健康有多种好处。在这种背景下,亲生物设计的概念越来越受到环境心理学研究者的关注。一些研究表明,绿色环境对男性和女性的积极影响可能不同。因此,本研究旨在探讨亲生物设计元素(即绿色项目和自然光)对患者自评心理健康价值、医疗服务满意度和对医疗机构忠诚度的影响。该研究还调查了性别差异对变量之间关系的可能影响。方法:采用结构方程模型作为数据分析技术。结果:亲生物设计元素显著提高了患者自评心理健康价值,且该维度对患者的医疗服务满意度和对医疗机构的忠诚度有正向影响。研究结果显示,亲生物设计元素、自评心理健康价值、医疗服务满意度和对医疗机构的忠诚度之间的关系在男性和女性群体中存在显著差异。此外,自评心理健康价值和医疗服务满意度在亲生物设计元素与忠诚度之间起显著中介作用。结论:本研究的结果为医疗从业者和研究人员提供了有效地将亲生物设计元素融入医疗机构内部空间的宝贵策略。
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引用次数: 2
Effect of Rehydration with Mineral Water on Cardiorespiratory Fitness Following Exercise-Induced Dehydration in Athletes 矿泉水补液对运动员运动性脱水后心肺功能的影响
IF 0.7 4区 医学 Q4 Medicine Pub Date : 2022-10-25 DOI: 10.31083/j.jomh1810206
Talal M. AL-Qurashi, K. Aljaloud, Abdulaziz M. Aldayel, Y. Alsharif, A. Alaqil, G. Alshuwaier
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引用次数: 2
SARS-CoV-2 Spike Protein Influences Expression of ICOSL and ICAM-2 in Prostate Cancer SARS-CoV-2刺突蛋白对癌症前列腺ICOSL和ICAM-2表达的影响
IF 0.7 4区 医学 Q4 Medicine Pub Date : 2022-10-18 DOI: 10.31083/j.jomh1810201
McKay Echols, Zuliang Deng, Coby G. D. Powers, HuaPing Xiao, Ziwen Zhu, Marco Lequio, Samuel Leung, Qian Bai, M. Wakefield, Yujiang Fang
Background: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the novel coronavirus responsible for the COVID-19 pandemic. The viral protein of SARS-CoV-2, spike protein (SP), mediates entry into host cells, contributing to pathogenesis of COVID-19. Prostate cancer is the most common cancer among men in the United States. Inducible T-cell costimulator ligand (ICOSL) and intercellular cell adhesion molecule 2 (ICAM-2) are expressed in cancer cells and their roles in cancer growth remain controversial. It is unknown if SP can affect the expression of ICAM-2 or ICOSL in prostate cancer. This study investigated the effects of SARS-CoV-2 SP on the expression of ICAM-2 and ICOSL and the time-dependent effect of SP on growth and survival of prostate cancer cells. Methods: The effect of SARS-CoV-2 SP on the survival of a widely-used prostate cancer cell line, LNCaP, was assessed using clonogenic cell survival assay and quick cell proliferation assay. Reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry (IHC) were performed to investigate the expression of ICAM-2 and ICOSL. The survival of an additional prostate cancer cell line, PC-3, was also evaluated by clonogenic survival assay. Results: After 3 days, a significant decrease in the percentage of colonies in LNCaP cells treated with SP was found, which was paralleled by a decrease in optical density (OD) value in LNCaP cells in the presence of SP. A significant decrease in the percentage of colonies treated with SP was also found in PC-3 cells evaluated by clonogenic survival assay. In addition, the mRNA expression of ICAM-2 was lower, whereas the mRNA expression of ICOSL was higher in SP-treated LNCaP cells. This was supported by protein expressions for ICAM-2 and ICOSL evaluated with IHC. Conclusions: In LNCaP cells, SARS-CoV-2 SP downregulates the expression of ICAM-2 but upregulates the expression of ICOSL. SARS-CoV-2 SP inhibits growth of prostate cancer cells in a time-dependent manner. Further studies are needed to fully address the roles of ICAM-2 and ICOSL in the inhibition prostate cancer growth by SARS-CoV-2 SP.
背景:严重急性呼吸综合征冠状病毒2 (SARS-CoV-2)是导致COVID-19大流行的新型冠状病毒。SARS-CoV-2的病毒蛋白刺突蛋白(spike protein, SP)介导进入宿主细胞,参与COVID-19的发病机制。前列腺癌是美国男性中最常见的癌症。诱导型t细胞共刺激配体(ICOSL)和细胞间细胞粘附分子2 (ICAM-2)在癌细胞中表达,但它们在肿瘤生长中的作用仍存在争议。SP是否能影响前列腺癌中ICAM-2或ICOSL的表达尚不清楚。本研究探讨了SARS-CoV-2 SP对前列腺癌细胞ICAM-2和ICOSL表达的影响以及SP对前列腺癌细胞生长和存活的时间依赖性。方法:采用克隆细胞存活试验和快速细胞增殖试验,观察SARS-CoV-2 SP对前列腺癌细胞LNCaP存活的影响。采用逆转录聚合酶链反应(RT-PCR)和免疫组化(IHC)检测ICAM-2和ICOSL的表达。另外一种前列腺癌细胞系PC-3的存活也通过克隆生存试验进行了评估。结果:SP作用3天后,LNCaP细胞的菌落百分比显著降低,SP作用下LNCaP细胞的光密度(OD)值也显著降低。克隆生存实验显示PC-3细胞中SP作用后的菌落百分比也显著降低。此外,sp处理的LNCaP细胞ICAM-2 mRNA表达量较低,ICOSL mRNA表达量较高。IHC检测的ICAM-2和ICOSL蛋白表达支持了这一点。结论:在LNCaP细胞中,SARS-CoV-2 SP下调ICAM-2的表达,上调ICOSL的表达。SARS-CoV-2 SP以时间依赖性方式抑制前列腺癌细胞的生长。ICAM-2和ICOSL在SARS-CoV-2 SP抑制前列腺癌生长中的作用有待进一步研究。
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引用次数: 1
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Journal of Men's Health
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