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CONTENTS Volume 74, Issue 12, December 2025. 目录74卷,12期,2025年12月。
IF 1.8 4区 农林科学 Q3 CHEMISTRY, APPLIED Pub Date : 2025-01-01 DOI: 10.5650/jos.74contents12
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引用次数: 0
Pulsed Microwave-Induced Growth Enhancement of Euglena gracilis Microalgae - Approach for Improved Industrial Cultivation. 脉冲微波诱导细叶藻微藻生长的研究——改进工业栽培的方法。
IF 1.8 4区 农林科学 Q3 CHEMISTRY, APPLIED Pub Date : 2025-01-01 DOI: 10.5650/jos.ess25152
Satoshi Horikoshi, Shurei Kawasako, Nick Serpone

This study investigates the effects of microwave radiation on Euglena gracilis (Euglena) during brief exposure periods, as well as the subsequent enhancement of growth under different conditions. The growth patterns of Euglena in these environments were systematically analyzed. Additionally, the study examined whether the growth rate of microwave-irradiated heterotrophic cultures of Euglena could be improved with 24-hour or shorter light exposure, which is an important factor in the industrial production of this organism. Results indicated that a 1-minute pulse of microwave radiation caused a twofold increase in growth compared to the control, even under autotrophic culture conditions without added CO2. Furthermore, there was a significant 2.6-fold increase in growth when daily light exposure was reduced from 24 to 10 hours.

本文研究了微波辐射在短时间内对细叶榆(Euglena gracilis)的影响,以及不同条件下对其生长的促进作用。系统分析了绿藻在这些环境中的生长规律。此外,本研究还考察了24小时或更短的光照是否能提高Euglena异养培养物的生长速度,这是该生物工业化生产的一个重要因素。结果表明,即使在不添加CO2的自养培养条件下,1分钟的微波辐射脉冲也能使生长速度比对照提高两倍。此外,当每日光照从24小时减少到10小时时,生长显著增加2.6倍。
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引用次数: 0
Analysis of Volatile Organic Compounds in Olive Oil of 'Koroneiki' with Different Maturity Indices by GC-IMS. 不同成熟度指标的“Koroneiki”橄榄油中挥发性有机物的GC-IMS分析
IF 1.6 4区 农林科学 Q3 CHEMISTRY, APPLIED Pub Date : 2025-01-01 DOI: 10.5650/jos.ess24339
Hui-Min Wang, Hong-Jie Zhang, Jun-Yi Ma, You-Qiong Liu, Wei-Bao Kong

This study aims to determine the optimal harvest period of olives by distinguishing the olive oils with different fruit maturity indices (MIs). Gas chromatography ion-mobility spectrometry (GC-IMS) technology was employed to qualitatively and differently analyze the volatile organic compounds (VOCs) of olive oil extracted from eight MIs of 'Koroneiki' olive fruits, harvested in Longnan City, Gansu Province, China. The results showed that 40 signal peaks were isolated in the eight olive oils with different MIs, and 33 VOCs were identified. These include alcohols (7 kinds), esters (7 kinds), aldehydes (6 kinds), ketones (5 kinds), acids (2 kinds), olefins (2 kinds), and other compounds (4). A total of 20 differential markers for key flavors, with variable importance in the projection (VIP) > 1, were screened out by orthogonal partial least squares - discriminant analysis (OPLS-DA). The results showed that the olive oil samples of the 7th maturity index (QJ7), QJ8, and QJ5, QJ6 have significant differences from the other four olive oils. This suggests that olive oils with different maturity indices can be effectively distinguished.

本研究旨在通过区分不同果实成熟度指数(MIs)的橄榄油,确定橄榄的最佳采收期。采用气相色谱-离子迁移谱(GC-IMS)技术对甘肃陇南产的“Koroneiki”橄榄果实中8种橄榄油的挥发性有机化合物(VOCs)进行了定性和差异性分析。结果表明,从8种不同MIs的橄榄油中分离出40个信号峰,鉴定出33种挥发性有机化合物。包括醇类(7种)、酯类(7种)、醛类(6种)、酮类(5种)、酸类(2种)、烯烃类(2种)和其他化合物(4种)。采用正交偏最小二乘-判别分析(OPLS-DA)筛选出20个在投影(VIP) >1中具有可变重要性的关键风味差异标记。结果表明,橄榄油样品的第7成熟度指标(QJ7)、QJ8和QJ5、QJ6与其他4种橄榄油有显著差异。说明不同成熟度指标的橄榄油可以有效区分。
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引用次数: 0
A Biotechnological Approach to Insect Waste Valorization: Silkworm Pupae as a Functional Feed Ingredient for Pangasius pangasius, Improving Growth and Physiological Responses. 昆虫废物增值的生物技术途径:蚕蛹作为巴沙鱼的功能性饲料成分,改善生长和生理反应。
IF 1.8 4区 农林科学 Q3 CHEMISTRY, APPLIED Pub Date : 2025-01-01 DOI: 10.5650/jos.ess25093
Ayesha Tariq, Sajida Naseem, Mehwish Faheem, Riffat Iqbal, Samia Majid, Fakhar-Un-Nisa Yunus, Abid Ali, Muhammad Nauman Aftab, Sikander Ali, Iram Liaqat

The rising cost of fish meal and soybean meal for aquafeeds has encouraged researchers to look for alternative protein sources. To find an alternative, this study was designed to replace soybean meal with silkworm pupae meal. An experimental feeding trial of 75 days was conducted on Pangasius fingerlings to assess the effects of partially substituted soybean meal with silkworm pupae meal (SWPM) on growth performance, antioxidant activity, and digestive enzymes. Four isonitrogenous diets were made with 0% SWPM as control and 25%, 50%, and 75% silkworm pupae meal as experimental diets. The fingerlings were given control and experimental diets at 3% of their body weight. The study found that the groups given SWPM at 25% and 50% concentration had the best growth performance, feed efficiency (up to 0.80±0.00%), and feed conversion ratio (up to 1.24±0.00%). Both groups showed increased highest final weight (up to 22.25±0.76), weight gain % (up to 95.03±0.35%), and improved survival growth rates (100%) as compared to the control. The control and treated groups (25% and 50%) exhibited significant differences (p < 0.05) in terms of antioxidant activity and digestive enzymes. The histomorphometry examination revealed no damage in hepatic tissues, intestinal villi, and podocytes as a result of the inclusion of silkworm pupae meal. The data suggest that silkworm pupae meal may serve as an adequate replacement for soybean meal, up to 50%, without negatively impacting the growth responses, antioxidant, and digestive enzyme activities of Pangasius.

作为水产饲料的鱼粉和豆粕价格的上涨促使研究人员寻找替代蛋白质来源。为了寻找蚕蛹粉替代豆粕的替代品,本研究设计了蚕蛹粉替代豆粕的方法。为研究蚕蛹粕部分替代豆粕(SWPM)对巴沙鱼鱼种生长性能、抗氧化活性和消化酶的影响,进行了75 d的试验饲养试验。以0% SWPM为对照,25%、50%和75%蚕蛹粉为试验饲料,配制4种等氮饲粮。分别饲喂体重3%的对照饲料和试验饲料。研究发现,25%和50% SWPM组生长性能最佳,饲料效率最高(0.80±0.00%),饲料系数最高(1.24±0.00%)。与对照组相比,两组的最高末重(22.25±0.76)、增重%(95.03±0.35%)和成活率(100%)均有提高。对照组(25%)和处理组(50%)在抗氧化活性和消化酶方面差异显著(p < 0.05)。组织形态学检查显示,蚕蛹粉未对肝组织、肠绒毛和足细胞造成损伤。这些数据表明,蚕蛹粉可以替代50%的豆粕,而不会对巴沙鱼的生长反应、抗氧化和消化酶活性产生负面影响。
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引用次数: 0
Effects of Addition of 1,3-Dipalmitoyl-2-palmitoleoyl-sn-glycerol on the Physical Properties of Cocoa Butter and Fat Bloom Stability of Dark Chocolate. 添加1,3-双棕榈酰-2-棕榈酰-sn-甘油对可可脂物理性质和黑巧克力脂肪开花稳定性的影响。
IF 1.8 4区 农林科学 Q3 CHEMISTRY, APPLIED Pub Date : 2025-01-01 DOI: 10.5650/jos.ess25105
Shimpei Watanabe, Kenji Ishiwari, Shinichi Yoshikawa, Kiyotaka Sato

Fat bloom is a serious problem that occurs when chocolate and related products are stored at high temperatures for long periods. Various techniques have been used to retard and prevent the fat bloom phenomenon. In this study, we investigated the ability of a new type of symmetrical triacylglycerol, 1,3-dipalmitoyl-2-palmitoleoyl-sn-glycerol (PPoP), containing palmitic acid (P) at positions sn-1 and sn-3 and palmitoleic acid (Po) at position sn-2, to retard or prevent fat bloom formation. An XRD study of cocoa butter (CB) and TAG mixtures of CB/PPoP and CB/POP revealed that CB and CB/POP mixtures transformed into polymorphic Form VI under conditions where the storage temperature was gradually raised from 20°C to 28°C over a period of 100 days. In contrast, the XRD patterns of CB/PPoP mixtures after 100 days of storage was similar to that of Form V of CB, and unlike that of Form VI of CB. These results are the first to indicate that the adding PPoP may prevent or delay the polymorphic transformation of CB from Form V to Form VI. We also investigated the physical properties and fat bloom stability of cocoa butter equivalent (CBE) and dark chocolates made with different PPoP concentration and found that the solid fat contents of CBE decreased with increasing amounts of PPoP. the addition of PPoP tended to soften the dark chocolate. Finally, we found that the fat bloom formation of dark chocolate under thermal cycle storage conditions between 18°C-30.5°C and 20°C-32°C could be retarded by adding of PPoP. However, further studies are warranted to elucidate the PPoP-induced retarding mechanisms during the transition from Form V to Form VI.

当巧克力和相关产品在高温下长时间储存时,脂肪开花是一个严重的问题。各种技术已被用来延缓和防止脂肪开花现象。在这项研究中,我们研究了一种新型对称三酰基甘油,1,3-二棕榈酰-2-棕榈酰-sn-甘油(PPoP),在sn-1和sn-3位置含有棕榈酸(P),在sn-2位置含有棕榈油酸(Po),以延缓或防止脂肪华形成的能力。对可可脂(CB)和TAG的CB/PPoP和CB/POP混合物的XRD研究表明,在贮存温度从20℃逐渐升高到28℃的100天条件下,CB和CB/POP混合物转变为多晶型VI。相比之下,CB/PPoP混合物在贮存100天后的XRD谱图与CB的V型相似,而与CB的VI型不同。这些结果首次表明,添加PPoP可以阻止或延缓CB从形态V向形态VI的多态转化。我们还研究了添加不同PPoP浓度的可可脂当量(CBE)和黑巧克力的物理性质和脂肪开花稳定性,发现CBE的固体脂肪含量随着PPoP添加量的增加而降低。PPoP的加入会使黑巧克力变软。最后,我们发现在18°C-30.5°C和20°C-32°C的热循环贮存条件下,添加PPoP可以延缓黑巧克力脂肪华的形成。然而,需要进一步的研究来阐明ppop诱导的从V型到VI型转变的延缓机制。
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引用次数: 0
Precipitation-Dissolution Phase Diagrams of Lead Halide Perovskites in Solutions for Single Crystallization in Capillaries. 卤化铅钙钛矿在毛细管单晶溶液中的沉淀-溶解相图。
IF 1.8 4区 农林科学 Q3 CHEMISTRY, APPLIED Pub Date : 2025-01-01 DOI: 10.5650/jos.ess25200
Satoshi Watanabe, Shun Inouchi, Tenyu Date

Organic-inorganic halide perovskite materials have attracted significant attention in the field of optoelectronics, and their single crystals, in particular, exhibit high carrier mobility. However, because single crystals were typically grown from highly polar donor solvents, the crystallization process was complicated by the formation of complexes, solvated crystals, and polymorphs. Despite these complexities, many studies have relied on empirical trial-and-error approaches, without systematically integrating the crystallization process into crystal engineering through phase diagrams. In this study, we constructed a phase diagram of methylammonium lead iodide perovskite and clarified its solubility and precipitation curves. From van't Hoff analysis, we determined the enthalpy and entropy of dissolution. Based on the supersaturation conditions obtained from the precipitation and solubility curves, we successfully grew single crystals. These insights are expected to provide important guidance for the fabrication of solar cells, photodetectors, and integrated devices.

有机-无机卤化物钙钛矿材料在光电子学领域引起了广泛的关注,特别是其单晶具有较高的载流子迁移率。然而,由于单晶通常是从高极性的供体溶剂中生长出来的,因此结晶过程由于络合物、溶剂化晶体和多晶的形成而变得复杂。尽管存在这些复杂性,但许多研究都依赖于经验试错方法,而没有通过相图系统地将结晶过程整合到晶体工程中。在本研究中,我们构建了甲基碘化铅钙钛矿的相图,并澄清了其溶解度和沉淀曲线。通过范霍夫分析,我们确定了溶解的焓和熵。根据沉淀和溶解度曲线得到的过饱和条件,我们成功地生长出了单晶。这些发现有望为太阳能电池、光电探测器和集成器件的制造提供重要的指导。
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引用次数: 0
Hydrolyzed Polyphenols from Water Chestnut (Trapa japonica) Suppress Lipase Activity in vitro and Blood Triacylglycerols in Mice. 从荸荠(Trapa japonica)中提取的水解多酚可抑制小鼠体外脂肪酶活性和血液中的三酰甘油。
IF 1.6 4区 农林科学 Q3 CHEMISTRY, APPLIED Pub Date : 2025-01-01 DOI: 10.5650/jos.ess24196
Midori Yasuda, Kenichiro Yasutake, Daichi Oikawa, Madoka Saiki, Ai Mitsuta-Takeyama

Water chestnut (Trapa japonica) is a thorny aquatic floating plant that contains hydrolyzable polyphenols (WCPs) such as eugeniin, 1,2,3,6-tetra-O-galloyl-β-d-glucopyranose (TGG), and trapain. Previous studies have revealed that WCPs have antioxidant properties and can suppress postprandial blood glucose elevation in mice and human participants. However, the functionality of WCPs in treating obesity and metabolic syndrome, which have become significant issues in recent years, remains unclear. Here, we aimed to determine the effects of WCPs on lipid metabolism. We initially investigated the effects of WCPs on pancreatic lipase in vitro. Among WCPs, eugeniin and trapain, which contain a hexahydroxybiphenyl group, particularly inhibited lipase activity. We also found that WCPs suppressed fat accumulation in mouse adipose progenitor 3T3-L1 cells. We compared the effects of WCPs on lipid metabolism among groups of mice (n=7 each) fed for four weeks without (standard control and high-fat (HF) diet controls) or with HFWP 0.5 or HFWP 1 supplemented with 0.5 and 1% WCPs, respectively. Subsequently, we examined lipid-related parameters in adipose tissue, liver, and blood. We found significantly lower plasma triacylglycerol content in the HFWP1 than in the HF group. These findings suggest that WCPs may help prevent the onset of obesity and metabolic syndrome.

荸荠(Trapa japonica)是一种多刺的水生漂浮植物,含有可水解的多酚(wcp),如eugeniin, 1,2,3,6-四- o -没食子酰-β-d-葡萄糖吡喃糖(TGG)和trapain。先前的研究表明,WCPs具有抗氧化特性,可以抑制小鼠和人类餐后血糖升高。然而,wcp在治疗肥胖和代谢综合征方面的功能,近年来已成为重要问题,尚不清楚。在这里,我们的目的是确定WCPs对脂质代谢的影响。我们在体外初步研究了WCPs对胰脂肪酶的影响。在wcp中,含有六羟基联苯基团的eugeniin和trapain对脂肪酶活性有明显的抑制作用。我们还发现WCPs抑制小鼠脂肪祖细胞3T3-L1的脂肪积累。我们比较了各组小鼠(每组n=7)饲喂4周不饲喂(标准对照和高脂(HF)饲料对照)或饲喂HFWP 0.5或HFWP 1(分别添加0.5和1% WCPs)的脂质代谢的影响。随后,我们检查了脂肪组织、肝脏和血液中的脂质相关参数。我们发现HFWP1组的血浆甘油三酯含量明显低于HF组。这些发现表明,WCPs可能有助于预防肥胖和代谢综合征的发生。
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引用次数: 0
In vitro Antioxidant and Antibacterial Activities, Bioactive Compounds, Organic Acids, Sugars, Micro- and Macro-elements of Aquilaria agallocha Root Organic Extracts. Aquilaria agallocha 根有机提取物的体外抗氧化和抗菌活性、生物活性化合物、有机酸、糖类、微量和宏观元素。
IF 1.6 4区 农林科学 Q3 CHEMISTRY, APPLIED Pub Date : 2025-01-01 DOI: 10.5650/jos.ess24228
Hatice Feyza Akbulut

Aquilaria agallocha is an economically valuable plant facing endangerment, sought after globally for its production of agarwood. A. agallocha tree possesses medicinal and aromatic properties in its fruits, branches, leaves, wood, and roots, which are being studied for their effective compounds and their potential bioactive effects on human health. This study aimed to uncover the phytochemical properties and biological activities of A. agallocha roots, which possess medicinal and aromatic characteristics. The phenolic, organic acid, and sugar profiles of A. agallocha roots were determined using HPLC-DAD, while mineral distributions were analyzed via ICP-AES. In vitro antioxidant capacity was assessed using the 2,2-diphenyl- 1-picrylhydrazyl (DPPH) assay. Furthermore, the antibacterial effects of methanol extracts of A. agalocha roots against S. aureus, S. carnosus, B. cereus, L. monocytogenes, E. coli, L. innocua, K. pneumoniae, and E. faecalis were estimated with disc diffusion and MIC methods. The amount of total phenolic and in vitro DPPH radical scavenging capacity of A. agalocha roots were determined as 7.529 mg GAE/g and 18.83 µmol TE/g, respectively. Eight phenolic components were detected in the extracts of A. agalocha roots. The most dominant among these phenolic compounds detected was tannic acid, which was followed by 4-hydrobenzoic acid and oleuropein, respectively. In the aqueous extracts of A. agalocha roots, four organic acids were identified, including citric, tartaric, malic and succinic acid, and succinic acid was the most dominant organic acid. Only fructose sugar was detected in the water extracts of A. agalocha roots. The extracts of A. agalocha roots had a strong antibacterial effect against all gram-positive pathogens except for S. carnosus, but did not have any effect against gram-negative bacteria.

沉香木香是一种濒临灭绝的具有经济价值的植物,因其生产沉香木而在全球范围内受到追捧。沉香树在其果实、树枝、叶子、木材和根中具有药用和芳香的特性,正在研究其有效化合物及其对人类健康的潜在生物活性作用。本研究旨在揭示具有药用和芳香特性的沉香根的植物化学特性和生物活性。采用HPLC-DAD法测定了沉香根的酚类、有机酸和糖谱,采用ICP-AES法分析了矿物质的分布。采用2,2-二苯基- 1-苦味酰肼(DPPH)法测定体外抗氧化能力。此外,采用碟扩散法和MIC法研究了沉香根甲醇提取物对金黄色葡萄球菌、肉毒杆菌、蜡样芽孢杆菌、单核增生乳杆菌、大肠杆菌、无性乳杆菌、肺炎克雷伯菌和粪肠杆菌的抑菌效果。测定沉香根总酚含量为7.529 mg GAE/g,体外清除DPPH自由基能力为18.83µmol TE/g。在沉香根提取物中检测到8种酚类成分。这些酚类化合物中最主要的是单宁酸,其次是4-羟基苯甲酸和橄榄苦苷。在沉香根水提液中,鉴定出柠檬酸、酒石酸、苹果酸和琥珀酸4种有机酸,其中琥珀酸是最主要的有机酸。芦荟根水提取物中仅检测到果糖。芦荟根提取物对除肉毒杆菌外的革兰氏阳性病原菌均有较强的抑菌作用,而对革兰氏阴性菌无抑菌作用。
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引用次数: 0
Growth Stage-Dependent Polyphenol Profiles and Antioxidant Properties in Medicinal Plants of Iraqi Kurdistan. 伊拉克库尔德斯坦药用植物生长阶段相关多酚谱及抗氧化特性
IF 1.6 4区 农林科学 Q3 CHEMISTRY, APPLIED Pub Date : 2025-01-01 DOI: 10.5650/jos.ess24263
Mohammad Saadatian, Samiaa Jamil Abdulwahid-Kurdi, Kadhim Sedeeq Mohammed, Rawen Abdulhadi Abdullah

Medicinal plants, rich in polyphenolic compounds, play a crucial role in traditional and modern medicine due to their antioxidant, anti-inflammatory, and disease-preventing properties. This study investigated the polyphenol content and antioxidant activity across various growth stages (pre-flowering, flowering, and post-flowering) of four medicinal plants: Primula auriculata L., Stachys vulgaris L., Verbascum spp., and Ajuga oblongata L., cultivated in the Soran region, Erbil Province, Iraqi Kurdistan. Using high-performance liquid chromatography (HPLC), nine types of polyphenols were identified and quantified, including gallic, caffeic, and chlorogenic acids. The results showed significant variations in polyphenol concentrations across different plant species and growth stages. For example, Primula exhibited the highest levels of gallic acid (41.91 mg/kg) during pre-flowering, while Ajuga showed peak chlorogenic acid content (827.62 mg/kg) post-flowering. Antioxidant activity was highest in Ajuga during flowering (75.33%) and lowest during post-flowering. Overall, polyphenol content was positively correlated with antioxidant activity, highlighting the importance of growth stage in optimizing the medicinal benefits of these plants. These findings contribute to the ethnobotanical knowledge of Kurdish medicinal plants and offer insights into the optimal harvest times for maximizing bioactive compounds.

药用植物富含多酚类化合物,由于其抗氧化、抗炎和预防疾病的特性,在传统和现代医学中发挥着至关重要的作用。研究了伊拉克库尔德斯坦省索兰地区4种药用植物Primula auriculata L.、Stachys vulgaris L.、Verbascum spp.和Ajuga oblongata L.在开花前、开花和开花后不同生长阶段(开花前、开花后)的多酚含量和抗氧化活性。采用高效液相色谱法(HPLC)对九种多酚进行了鉴定和定量,包括没食子酸、咖啡酸和绿原酸。结果表明,不同植物种类和不同生长阶段多酚浓度存在显著差异。报春花的没食子酸含量在开花前最高(41.91 mg/kg),而绿原酸含量在开花后最高(827.62 mg/kg)。开花期间抗氧化活性最高(75.33%),花后抗氧化活性最低。综上所述,多酚含量与抗氧化活性呈正相关,说明了生长阶段对优化这些植物药用价值的重要性。这些发现有助于库尔德药用植物的民族植物学知识,并为最大化生物活性化合物的最佳收获时间提供见解。
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引用次数: 0
CONTENTS Volume 74, Issue 1, January 2025. 目录74卷,第1期,2025年1月。
IF 1.6 4区 农林科学 Q3 CHEMISTRY, APPLIED Pub Date : 2025-01-01 DOI: 10.5650/jos.74contents1
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引用次数: 0
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Journal of oleo science
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