Journal of oleo science最新文献

In this study, the effects of different cooking processes on the phytochemical profile and mineral content of garlic (Allium sativum L.) were determined. Different cooking processes had a significant effect on the moisture, pH, water-soluble solids, L*, a*, b*, C* and °h values. The phenolic profile was generally better preserved in the steamed garlic samples than in the control samples. The mineral content of garlic prepared different cooking processes, with the exception of copper and magnesium, was found to be relatively high. As a result, the phenolic acid and mineral contents of garlic samples prepared with different cooking processes were better preserved and were closest to those of the control samples. In addition, to preserve the valuable compounds in garlic, adding them to dishes after the cooking process is completed is recommended.

Adding of vegetable oils to skincare products or the use of plant oils for oil care is a current trend. Therefore, the safety and functionality of vegetable oils are of great concern to consumers and cosmetics manufacturers. This study focused on three types of vegetable oils: sunflower oil (SO), andiroba oil (AO) and hydrogenated olive oil (HOO). We conducted a comprehensive evaluation of the oils, which encompassed their ability to protect mouse skin keratinocytes (XB-2) and mouse fibroblasts (NIH 3T3) from damage caused by the surfactant sodium lauryl sulfate (SLS), their influence on the levels of filaggrin and collagen, their potential to aid in wound healing, and their effectiveness in anti-Staphylococcus aureus biofilm formation. The results showed that SO, AO and HOO at a concentration of 1.5 × 10^-4 % (v/v) have the ability to defend against SLS-induced cell damage, increase wound healing ability and the filaggrin and collagen content to XB-2 or NIH 3T3 cells. SO, AO and HOO at a concentration of 3.75 × 10^-3 % also have the anti-biofilm ability. Among the oils, AO can inhibit S. aureus biofilm composed of either polysaccharides or proteins. Therefore, the tested vegetable oils and can be applied to the cosmetics field as ingredients to repair damaged skin and preserve skin barrier stability.

In the present study, the mixed micellization behavior of gemini surfactant-1, 5-bis (N-hexadecyl- N, N-dimethylammonium) pentane dibromide (G5) with non-ionic surfactant triton X-100 (TX-100) was investigated in the micellar phase by utilizing the conductometric technique. The deviation of ideal critical micelle concentration (cmc*) from experimental critical micelle concentration (cmc) has been estimated using well-known Clint's theory of mixed micelles. The regular solution approximation was used to determine the interaction parameter (β) and found to be negative. The negative values of β at all mole fractions confirm an attractive interaction between two mixed components. The activity coefficients and excess Gibbs free energy of mixed micelles have been calculated by using different approximations, like Rubingh, Lange and Motomura. Counterion binding (g) computed from the post and premicellar slopes of specific conductance vs. concentration graph. Overall, in most of cases, in presence of TX-100, the counterion binding of gemini surfactant was found to be less in magnitude. The molecular interaction was also investigated by the density function theory (DFT). A polarizable continuum model (PCM) was used (with water as a solvent) to optimize the single surfactants and their mixture. The computational process was carried out by the B3LYP method and the 6-31G basis set.

Bile salts, present in the gastrointestinal tract as biosurfactants, play a crucial role in emulsifying and solubilizing fat-soluble nutrients and drugs, thereby facilitating their absorption. However, the cellular permeation of bile acid-mixed micelles solubilized with lipophilic substances remains inadequately explored. To comprehend the cell permeation behavior of bile salts and their mixed micelles, giant unilamellar vesicles (GUVs) were employed as a cell-mimetic system, prepared with dioleylphosphatidylcholine (DOPC). Confocal laser scanning microscopy, utilizing fluorescent dyes doped in the lipid membrane and solubilized substances, was employed to observe morphological changes in GUVs subsequent to the application of sodium cholate (NaC) alone and NaC-mixed micelles solubilized with lipophilic components. In the case of NaC alone, below the critical micelle concentration (CMC), the monomer interacts with the lipid membrane of the GUV, inducing endocytic morphological changes that result in the formation of small vesicles containing the bulk liquid inside the GUV. Conversely, when both monomers and micelles interacted with the lipid membrane beyond the CMC, lipid aggregates such as buds and threads protruded outward from the GUV. Contrastingly, upon application of three types of NaC mixed micelles-NaC-P solubilized with Palmitoyloleoylphosphocholine (POPC), NaC-P-F solubilized with oleic acid (OA) and monoolein (MO), and NaC-P-P solubilized with perylene, a liposoluble dye-to the GUV, the lipid membranes formed aggregates or vesicles and migrated into the interior of the GUV. In the case of NaC-P and NaC-P-P, the coexistence of drawn lipid aggregates and solubilized substances was scarcely observed. In contrast, for NaC-P-F, the coexistence of solubilized substances was observed in both lipid aggregates and small vesicles that migrated into the GUV. It is suggested that the partitioning of the solubilized substance from the mixed micelles adsorbed on the GUV to the lipid bilayer is implicated in the permeation of the solubilized substance through the cell membrane.

In this study, the effect of microwave drying on oil content, bioactive compounds, antioxidant activity, polyphenols and fatty acid profiles of fresh (control) and dried plum kernels was investigated. The oil quantities of plum seeds dried were found between 27.40% (control) and 42.42% (900 W). Total phenolic and flavonoid values of fresh (control) and dried-plum seeds were assessed to be between 9.77 (control) and 41.66 mgGAE/100 g (900 w) to 6.90 (control) and 23.67 mg/100 g (900 W), respectively. Total phenol and flavonoid quantities of the plum seeds dried at 900 W were slightly higher than those of the plum seeds dried at 720 W. L* (brightness) values of plum seeds changed between 55.97 and 59.62. Roasting in the microwave oven at 720 W was decreased the L* values of samples, while L* value of sample roasted at 900 W was closed to control. Gallic and 3,4-dihydroxybenzoic acid values of plum kernel samples were assigned to be between 1.19 (720 W) and 2.01 mg/100 g (900 W) to 0.22 (control) and 7.09 mg/100 g (900 W), respectively. Also, catechin and rutin quantities of plum seeds were established between 0.20 (control) and 7.55 mg/100 g (900 W) to 1.42 (control) and 3.59 mg/100 g (900 W), respectively. In general, the amount of phenolic compounds of plum seeds dried at every two watts showed an increase (except quercetin) compared to the control. Only the amount of quercetin decreased partially in the dried samples. While oleic acid quantities of raw (control) and dried plum kernel oils are reported between 68.28% (720 W) and 71.60% (900 W), linoleic acid amounts of plum kernel oils were found between 20.77% (900 W) and 23.49% (720 W). The quantities of saturated fatty acids in plum kernel oils were found to be quite low compared to the content of unsaturated fatty acids.

Near-infrared wavelength-selective soft actuators have attracted much attention for applications in microsystems in bioliving. It is desirable for the photothermal conversion materials in the actuators to be downsized to the molecular scale. However, in conventional actuator materials using copolymer gels composed of thermosensitive and photothermal conversion molecule-coordinated monomers, the strong cross-linking of molecules in the networks impairs the actuator deformation. In this study, we fabricated soft actuators consisting of interpenetrating polymer network (IPN) gels to suppress the cross-linking of the thermosensitive networks. Nd³⁺ and Yb³⁺ were used as wavelength-selective photothermal conversion molecules at 808 and 980 nm. Hydrophobic acrylamide derivatives and sodium acrylate were used as the thermosensitive and lanthanoid-ion-coordinated polymers, respectively. The lanthanoid ion concentrations in the IPN gels were about 0.2 M, which is 6 times larger than those of previous gels. The temperature response of swelling degrees (wt%) of the lanthanoid-ion-coordinated IPN gels were three times larger than that of the previous gels. Sandwich structure actuators consisting of Nd³⁺ and Yb ³⁺ IPN gels bent selectively toward the Nd ³⁺ gel side under 808 nm irradiation and toward the Yb³⁺ gel side under 980 nm irradiation.

Antibiotic resistance is a world wide problem mainly in developing countries. In this work, coelomic fluid (PCF) and paste (PBP) of Pheretima posthuma was assessed for its potential as antibiofilm and anti-quorum sensing (QS) agent against pathogenic bacterial biofilms. PCF and PBP were extracted and biofilm formation time kinetics was examined using crystal violet staining method by utilizing four bacterial isolates in bispecies biofilm (06 combinations; MH5-MH10) and multi species biofilms (05 combinations; MH11-MH15). QS study was performed by determining pyocyanin formation time kinetics using 03 P. aeruginosa strains at various time periods (0, 24, 48, 72, 96 and 120 hours). Following next, anti- QS effect was analyzed by measuring pyocyanin concentrations. Results showed that among bispecies bacterial biofilms, MH5-MH7 combinations showed significantly higher biofilm (p < 0.05) after 72 hours while other three (MH8-MH10) produced maximum biofilm after 48 to 72 hours. Likewise, for multispecies biofilms, maximum biofilm was noted after 48-72 hours. QS analysis revealed that PA1 strain synthesized highly significant (p < 0.001) pyocyanin (20 µg/mL) after 96 hours compared to PA2 and PA3 strains, which formed significant pyocyanin (18 µg/mL) after 72 hours. Also, 100-150 µg/mL of both PCF and PBP exhibited a significant (p < 0.05) reduction in biofilm while 200 µg/mL concentration showed highest QS against all selected strains. To our knowledge, this is the first assessment of its kind on the potential application of earthworm PCF and PBP for its antibiofilm and anti-QS potential. The study recommends precise chemistry of bioactive agents and their probable mechanism(s) of actions for the observed interference. Also, new pharmaceutical drugs synthesized using bioactive agents from PCF and PCB may surely have the potential to manage different infection problems caused by bacterial biofilms.

Tangeretin is one of the most abundant polymethoxyflavones in citrus peel and its pharmacological effects are extremely rich. However, due to its poor solubility, bitter taste and poor oral bioavailability, the oral administration of tangeretin is still limited, which seriously limits its application in industrial production. The establishment of encapsulation and delivery systems to improve bioavailability is an effective method. This paper reviewed the research progress of the structure and properties, pharmacological effects and main methods to improve bioavailability of tangeretin, including emulsion delivery, lipid encapsulation, microencapsulation and other delivery and utilization research and application. The article aims to provide theoretical basis for the high-value application of tangeretin in functional food and pharmaceutical industry.

In this study, the total phenol, total flavonoid content, antioxidant capacity, phenolic component and fatty acid profiles of caper seed oils extracted by solvent extraction, sonication extraction and cold press methods were revealed. Total phenol amounts of caper seed oils extracted by cold press, sonication and solvent systems were recorded as 0.10, 0.11 and 0.16 mg GAE/100 g, respectively. There was no statistically significant differences between the total phenol values of caper seed oils provided by sonication and cold press systems (p > 0.05). While the flavonoid amount of the oil extracted from caper seeds by solvent extraction system is determined as 358.9 mg CE/100 g, the total flavonoid amounts of caper seed oils extracted by sonication and cold pressing methods were established as 194.6 and 83.9 mgCE/100 g, respectively. The highest antioxidant capacity was established in the oil provided by solvent extraction (1.456%), followed by ultrasonic extraction (1.453%) and cold press oil (1.448%) in decreasing order. The dominant phenolic components of caper seed oils were quercetin, kaempferol, gallic acid, resveratrol and catechin. The fatty acid detected at the highest value in caper oils extracted by different extraction systems was linoleic acid (61.16-62.74%), followed by oleic, palmitic and stearic acids in decreasing order. Other fatty acids were recorded at low levels. As a result, it can be said that the caper oil extracted by solvent extraction is richer in quercetin and linoleic acid.