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JIS Reference Liquid Detergent Considering the Characteristics of Commercially Available Liquid Detergents. 考虑到市售液体洗涤剂的特性,JIS参考液体洗涤剂。
IF 1.8 4区 农林科学 Q3 CHEMISTRY, APPLIED Pub Date : 2026-01-01 DOI: 10.5650/jos.ess25110
Sumiko Goto, Masaru Oya, Satomi Kuwabara, Yoshitaka Miyamae, Isao Yamada, Masafumi Nomura, Ryo Hyodo

In recent years, Japan has been moving away from powder detergents to liquid detergents, but only powder types have been set as standard indicator detergents for JIS detergency tests. Therefore, optimizing the liquid reference detergent composition, the standard model for commercial liquid detergents in the JIS K 3362 revision for household synthetic detergents, was studied based on the detergency and mechanism of commercial liquid and powder detergents. A JIS standard liquid composition was proposed based on the AATCC liquid detergent composition (the 2003 AATCC standard reference liquid laundry detergent), and detergency evaluations were conducted using a Terg-O-Tometer using natural collar dirt-soiled cloth and various commercially available soiled cloths. As a result, in the cleaning experiment using collar dirt-soiled cloth, the new JIS liquid reference detergent showed the same detergency as the conventional JIS powder detergent and commercial liquid detergents. It also showed the same detergency as commercial liquid detergents for artificial sebum stains, indicating that it is suitable as an indicator detergent for commercial liquid detergents. However, it was confirmed that there are some points to note, such as the fact that liquid detergents have a weaker alkaline component and, therefore, have a lower detergency for artificial sebum stains than powder detergents and that the new JIS reference liquid detergent has a higher cleaning power for triacylglycerol stains than commercially available liquid and powder detergents due to their high surfactant concentration.

近年来,日本逐渐从粉状洗涤剂转向液体洗涤剂,但JIS去污力测试只将粉状类型作为标准指示洗涤剂。因此,基于商品液体和粉末洗涤剂的去污力和机理,对JIS K 3362修订家用合成洗涤剂中商品液体洗涤剂的标准模型——液体参考洗涤剂成分进行了优化研究。基于AATCC液体洗涤剂成分(2003 AATCC标准参考洗衣液)提出了JIS标准液体成分,并使用天然领脏布和各种市售脏布使用Terg-O-Tometer进行了去污力评估。结果表明,在领巾清洗实验中,新型JIS液体基准洗涤剂与常规JIS粉末洗涤剂和商用液体洗涤剂具有相同的去污力。对人造皮脂污渍的去污力与市售液体洗涤剂相同,适合作为市售液体洗涤剂的指示剂。然而,经证实,有一些要点需要注意,例如液体洗涤剂的碱性成分较弱,因此对人造皮脂污渍的去除率低于粉末洗涤剂,而新的JIS参考液体洗涤剂由于表面活性剂浓度高,对三酰甘油污渍的去除率高于市卖的液体和粉末洗涤剂。
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引用次数: 0
Treatment of Ulcerative Colitis with Myrrh Essential Oil Microemulsion. 没药精油微乳治疗溃疡性结肠炎。
IF 1.8 4区 农林科学 Q3 CHEMISTRY, APPLIED Pub Date : 2026-01-01 DOI: 10.5650/jos.ess25047
Mengmeng Wang, Tiantian Tang, Mei Liu, Zihao Niu, Le Zhang, Xuan Wang, Ning Xia, Jie Wang, Jinhui Wang, Chao Chen, Xiaoxiao Ge, Qin Chen, Shihao Zhang, Huanxian Shi, Yundong Xie, Chongbo Zhao, Junbo Zou, Dongyan Guo, Yajun Shi, Ming Yang, Xiaofei Zhang, Jing Sun

Ulcerative colitis (UC) is a chronic inflammatory colon disease that is a major public health problem. The long-term administration of traditional drugs is likely to trigger adverse reactions. The myrrh essential oil (MEO) exhibits promising therapeutic efficacy against ulcerative colitis. However, due to the inherent instability and volatility of MEO, it was formulated into myrrh essential oil microemulsion (MM) with the aim of enhancing its stability. This study explored the optimal formulation for synthesizing MM, and a series of relevant indices were employed to meticulously investigate its stability characteristics. In animal experiments, a UC mouse model was established with the assistance of Dextran Sulfate Sodium (DSS). The therapeutic efficacy was comprehensively evaluated by recording Disease Activity Index (DAI) scores, detecting the levels of Tumor Necrosis Factor-α (TNF-α) and Interleukin-1β (IL-1β), and observing the morphological features of mouse colonic tissues through hematoxylin-eosin (HE) staining and immunohistochemistry (IHC) techniques. The results confirmed that both MEO and MM are effective in treating ulcerative colitis.

溃疡性结肠炎(UC)是一种慢性炎症性结肠疾病,是一个主要的公共卫生问题。长期服用传统药物有可能引发不良反应。没药精油(MEO)显示出治疗溃疡性结肠炎的良好疗效。然而,由于MEO固有的不稳定性和挥发性,为了提高其稳定性,将其配制成没药精油微乳液(MM)。本研究探索了MM的最佳合成配方,并采用一系列相关指标对其稳定性特性进行了细致的考察。动物实验中,采用葡聚糖硫酸钠(DSS)辅助建立UC小鼠模型。通过记录疾病活动指数(DAI)评分,检测肿瘤坏死因子-α (TNF-α)和白细胞介素-1β (IL-1β)水平,并通过苏木精-伊红(HE)染色和免疫组化(IHC)技术观察小鼠结肠组织形态学特征,综合评价治疗效果。结果证实MEO和MM治疗溃疡性结肠炎均有效。
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引用次数: 0
Evaluation of Antioxidant Activity and Phenolic Content of Medicinal Plants Extracted by Ethanol, Methanol, and Water. 乙醇、甲醇和水提取药用植物抗氧化活性及酚类物质含量的评价
IF 1.8 4区 农林科学 Q3 CHEMISTRY, APPLIED Pub Date : 2026-01-01 DOI: 10.5650/jos.ess25164
Burcu Çakmak Sancar, Halil İbrahim Binici, Meryem Akhan

The aim of this study was to compare both the efficiency of different extraction solvents and the phenolic content of various plant species. Analyses included pH, total phenolic content (TPC), total flavonoid content (TFC), antioxidant capacity (FRAP, CUPRAC), and DPPH IC₅₀. Methanol extract pH ranged from hibiscus (Hibiscus sabdariffa L.) at 3.71 to 8.29 green tea leaf (Camellia sinensis L.). Quince leaf (Cydonia oblonga) water extract had the highest TPC (949.25 mg GAE/100 g) and flavonoid content (5166.21 mg QE/100 g). Lemon balm (Melissa officinalis) showed the highest FRAP and CUPRAC values in both methanol and water extracts. Rosehip (Rosa canina L.) water extract had the strongest DPPH activity (IC₅₀: 8.61 µg/mL). These findings suggest that lemon balm (Melissa officinalis), quince leaf (Cydonia oblonga), and rosehip (Rosa canina) have strong potential as functional food ingredients, owing to their rich phenolic and flavonoid content and potent antioxidant activities, which could be beneficial in preventing oxidative stress-related health conditions.

本研究的目的是比较不同提取溶剂的提取效率和不同植物中酚类物质的含量。分析包括pH值、总酚含量(TPC)、总黄酮含量(TFC)、抗氧化能力(FRAP、CUPRAC)和DPPH IC₅0。甲醇提取物pH值范围为芙蓉(hibiscus sabdariffa L.)为3.71 ~ 8.29,绿茶(Camellia sinensis L.)。木瓜叶水提物TPC (949.25 mg GAE/100 g)和黄酮类化合物含量(5166.21 mg QE/100 g)最高。香蜂草(Melissa officinalis)在甲醇和水提取物中FRAP和CUPRAC值最高。玫瑰果(Rosa canina L.)水提取物具有最强的DPPH活性(IC₅₀:8.61µg/mL)。这些发现表明,柠檬香蜂草(Melissa officinalis)、榅桲叶(Cydonia oblonga)和玫瑰果(Rosa canina)因其丰富的酚类和类黄酮含量以及强大的抗氧化活性,可能有助于预防与氧化应激相关的健康疾病,具有很强的功能性食品成分潜力。
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引用次数: 0
Immunomodulatory and Anti-Inflammatory Potential of Shark Liver Oil: A Scoping Review of Human and Animal Studies. 鲨鱼鱼肝油的免疫调节和抗炎潜力:人类和动物研究的范围综述。
IF 1.8 4区 农林科学 Q3 CHEMISTRY, APPLIED Pub Date : 2026-01-01 DOI: 10.5650/jos.ess25208
Dalila Najia Suhaimi, Mohd Hanif Zulfakar

Shark liver oil (SLO) is commercially promoted as an immunity booster to fight off infections, heal wounds, and for its beneficial effects in cancer treatment. Despite the growing body of research on SLO, scattered data and reported outcomes in understanding its immunomodulatory and anti-inflammatory mechanisms exist across study designs and populations, and the experimental design variances complicate data interpretation for meaningful translation into clinical applications. This scoping review aims to address this by comprehensively charting human and animal models to identify patterns of immunomodulatory and anti-inflammatory effects of SLO and its bioactive components, thus highlighting areas requiring further investigation. A scoping search through Cochrane, PubMed®, MEDLINE® via Ovid and Scopus using keywords such as "shark liver oil", "immunomodulation", "anti-inflammatory", similar keywords and their combinations was performed. Quantitative and qualitative measurements of immune or inflammatory responses were recorded and mapped. A total of 16 articles met the inclusion criteria, including human studies and in vivo animal models with various baseline health conditions. SLO has a broad yet selective immunomodulatory effect that shows an adaptive response depending on context, such as inflammatory conditions, cancer, or infection. The inconsistencies in results highlighted the biological complexity and the context-dependent role of SLO in the immune system. Despite this, alkylglycerols (AKG) emerge as a key bioactive component, with some studies suggesting potential dose-dependent effects and exploring the structure-activity relationship of different AKG forms in modulating the immune response. Overall, the current evidence is promising but preliminary, with methodological differences in the literature that highlight a clear need for rigorous, standardized clinical trials.

鲨鱼鱼肝油(SLO)在商业上被推广为免疫增强剂,可以抵抗感染,愈合伤口,并对癌症治疗有有益的影响。尽管对SLO的研究越来越多,但在了解其免疫调节和抗炎机制方面,不同研究设计和人群存在分散的数据和报告结果,并且实验设计差异使数据解释复杂化,难以有意义地转化为临床应用。本综述旨在通过全面绘制人类和动物模型来确定SLO及其生物活性成分的免疫调节和抗炎作用模式,从而突出需要进一步研究的领域。通过Ovid和Scopus对Cochrane、PubMed®、MEDLINE®进行检索,检索关键词为“鲨鱼肝油”、“免疫调节”、“抗炎”、类似关键词及其组合。记录免疫或炎症反应的定量和定性测量并绘制地图。共有16篇文章符合纳入标准,包括人体研究和具有各种基线健康状况的体内动物模型。SLO具有广泛而选择性的免疫调节作用,根据环境(如炎症、癌症或感染)表现出适应性反应。结果的不一致突出了生物复杂性和SLO在免疫系统中的环境依赖性作用。尽管如此,烷基甘油(AKG)作为一种关键的生物活性成分出现,一些研究表明潜在的剂量依赖效应,并探索不同形式的AKG在调节免疫反应中的构效关系。总的来说,目前的证据是有希望的,但只是初步的,文献中方法上的差异突出了严格的、标准化的临床试验的明确需要。
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引用次数: 0
Enzymatic Potential and Antifungal Susceptibility Profile of Aspergillus terreus Against Essential Oils. 土曲霉对精油的酶促电位及抗真菌敏感性分析。
IF 1.8 4区 农林科学 Q3 CHEMISTRY, APPLIED Pub Date : 2026-01-01 DOI: 10.5650/jos.ess25176
Saba Sana, Nida Sana, Areeba Nadeem, Layyaba Nazir, Rukhma Sattar, Sikander Ali, Najeeb Ullah, Rania Ali El Hadi Mohamed, Nawal Al-Hoshani, Maher S Alwethaynani, Fakhria A Al-Joufi, Tariq Aziz

Aspergillus terreus is a pathogen as well as an industrial organism. Current study deals with in vitro evaluation of A. terreus as a potential candidate for starch-based industries and susceptibility of toxigenic isolates to plant essential oils. Conventionally identified A. terreus soil isolates (n=25) were screened for mycotoxin production by TLC. Non- toxigenic isolates were selected for starch hydrolysis using starch agar. Toxigenic A. terreus were selected for antifungal activity of plant-derived essential oils (Nigella sativa, Elettaria cardamomum, Eucalyptus globulus, Cinnamomum verum Cuminum cyminum, and Syzygium aromaticum) by agar well diffusion and micro-broth dilution method. The lowest minimum inhibitory concentration (MIC ≥ 0.65±0.22µL/mL) was observed for Cuminum cyminum. So, it was used for inhibitory effect on growth and toxin production in broken grains. Cuminum cyminum EO inhibited the growth of A. terreus (0.00±0.00 cfu/g) in inoculated group and OTA production was also close to uninoculated group. Starch hydrolysis to colony diameter ratio helped for selection of isolates for further experiments. In starch hydrolysis, two isolates AST-01 and AST-02 produced highest ratios, which were 2.25±.08 and 1.76±.04 respectively. Influence of maize flour, wheat bran and rice husk with varying concentration, incubation temperature and pH were evaluated on starch hydrolysis potential. The hydrolytic potential quantified by dinitorosalisylic acid (DNS) method. A. terreus AST-02 had the highest hydrolytic potential (102.96±2.61IU) under incubation of 3% wheat bran at 37 °C and pH 6.0. It is concluded that A. terreus is a potential candidate for starch-based industries and further crop contamination by toxigenic species could be curtailed using essential oil as a feed preservative.

土曲霉是一种病原体,也是一种工业生物。目前的研究主要集中在体外评价田螺作为淀粉基工业的潜在候选物,以及产毒菌株对植物精油的敏感性。采用薄层色谱法对常规鉴定的土孢霉分离株(25株)进行霉菌毒素产率筛选。选择不产毒的菌株,用淀粉琼脂进行淀粉水解。采用琼脂孔扩散法和微肉汤稀释法对植物源性精油(黑鬼、小豆角、蓝桉、肉桂和香薷)的抑菌活性进行了筛选。最低抑菌浓度(MIC≥0.65±0.22µL/mL)。因此,它对破碎颗粒的生长和毒素产生具有抑制作用。接种组Cuminum cyminum EO抑制了A. terreus的生长(0.00±0.00 cfu/g), OTA产量也接近未接种组。淀粉水解与菌落直径比有助于进一步筛选分离菌株。在淀粉水解中,AST-01和AST-02的水解率最高,为2.25±。08和1.76±。04。考察了玉米粉、麦麸和稻壳不同浓度、培养温度和pH对淀粉水解电位的影响。二硝基水杨酸(DNS)法测定水解电位。在37℃、pH 6.0、3%麦麸条件下,A. terreus AST-02水解电位最高(102.96±2.61IU)。综上所述,土刺蒿是淀粉基工业的潜在候选者,使用精油作为饲料防腐剂可以减少毒素物种对作物的进一步污染。
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引用次数: 0
CONTENTS Volume 75, Issue 2, February 2026. 目录75卷,第2期,2026年2月。
IF 1.8 4区 农林科学 Q3 CHEMISTRY, APPLIED Pub Date : 2026-01-01 DOI: 10.5650/jos.75contents2
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引用次数: 0
Infrared Spectroscopic Analysis Indicates That Cocamidopropyl Betaine Has Little Effect on the Stratum Corneum. 红外光谱分析表明,椰油酰胺丙基甜菜碱对角质层的影响不大。
IF 1.8 4区 农林科学 Q3 CHEMISTRY, APPLIED Pub Date : 2026-01-01 DOI: 10.5650/jos.ess25210
Madoka Kage, Aki Oda, Yutaka Takagi

Cocamidopropyl betaine (CAPB) is an amphoteric surfactant that is commonly used in skin care materials. Although there are many reports about the safety of CAPB, there is limited data about its physical effects on the stratum corneum. Porcine stratum corneum sheets were soaked in 5% or 10% CAPB solution (pH 4.0, 7.0 or 9.0) for up to 60 min at room temperature. After rinsing with distilled water, the properties of the porcine stratum corneum sheets were analyzed using attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR). Sixty min of treatment with 5% CAPB or 10 min of treatment with 10% CAPB did not induce any peak shift at any pH. However, 60 min of treatment with 10% CAPB induced peak shifts of CH2 asymmetric stretching vibration and NH stretching vibration to higher wavenumber values. Although all pH solutions induced those peak shifts, the higher pH solutions caused larger peak shifts. However, 24 hrs treatment with 10% CAPB did not cause swelling at any pH. These results indicate that a long time and/or higher dose of CAPB affects the surface of stratum corneum, but this effect may not be caused by the regular use of CAPB in skin care materials.

椰油酰胺丙基甜菜碱(CAPB)是一种两性表面活性剂,常用于皮肤护理材料中。虽然有许多关于CAPB安全性的报道,但关于其对角质层的物理影响的数据有限。猪角质层片在5%或10% CAPB溶液(pH 4.0, 7.0或9.0)中室温浸泡60分钟。用蒸馏水冲洗后,利用衰减全反射-傅里叶变换红外光谱(ATR-FTIR)分析了猪角质层片的性质。5% CAPB处理60 min或10% CAPB处理10 min在任何ph值下均未引起峰移,但10% CAPB处理60 min引起CH2不对称拉伸振动和NH拉伸振动的峰移至更高的波数值。虽然所有pH溶液都能引起峰移,但pH值越高,峰移越大。然而,用10%的CAPB治疗24小时,在任何ph值下都没有引起肿胀。这些结果表明,长时间和/或更高剂量的CAPB会影响角质层表面,但这种影响可能不是由于在护肤材料中经常使用CAPB引起的。
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引用次数: 0
Design and Characterization of Food-Grade Liposomes for Enhanced Food Function and Delivery of p-Coumaric Acid Extracted from Sugarcane Bagasse. 食品级脂质体的设计与表征,用于增强甘蔗渣中对香豆酸的食物功能和输送。
IF 1.8 4区 农林科学 Q3 CHEMISTRY, APPLIED Pub Date : 2026-01-01 DOI: 10.5650/jos.ess25224
Naru Miyagi, Shuri Yamamoto, Makoto Takahashi

This study evaluated liposomes prepared using three food-grade lecithins-SLP-WHITE and SLP-PC70 (soy-derived), and PL-30S (egg yolk-derived)-to enhance the food function and delivery of p-coumaric acid (CA), a phenolic antioxidant extracted from sugarcane bagasse. Among the tested formulations, liposomes composed of SLP-WHITE and PL-30S at a 90:10 (w/w) ratio exhibited the highest encapsulation efficiency (82%). Notably, dynamic light scattering and confocal laser scanning microscopy revealed a strong negative zeta potential (-75.26 mV), small particle size (~100 nm), and a unilamellar structure, confirming this formulation as the optimum condition. Interestingly, encapsulated CA prepared with this optimum combination retained its antioxidant activity for 28 days. Furthermore, in a Caco-2 monolayer model, the liposomal CA showed significantly improved intestinal permeability compared to free CA, suggesting enhanced bioavailability via endocytic uptake. These results demonstrate that rational combinations of food-grade lecithins with complementary characteristics-such as the electrostatic stability of SLP-WHITE and membrane rigidity from PL-30S-can improve both physical stability and functionality of liposomes. This study provides a practical strategy for developing functional liposomal carriers suitable for food applications, particularly for delivering poorly bioavailable phenolic compounds like p-coumaric acid.

本研究评估了用三种食品级卵磷脂——slp - white和SLP-PC70(大豆衍生)以及PL-30S(蛋黄衍生)制备的脂质体,以增强对香豆酸(CA)的食物功能和传递,对香豆酸是一种从蔗渣中提取的酚类抗氧化剂。其中,SLP-WHITE与PL-30S以90:10 (w/w)比例组成的脂质体包封率最高(82%)。动态光散射和激光共聚焦扫描显微镜结果显示,该配方具有较强的负zeta电位(-75.26 mV)、较小的粒径(~100 nm)和单层结构,证实了该配方为最佳配方。有趣的是,以该最佳组合制备的胶囊化CA的抗氧化活性保持了28天。此外,在Caco-2单层模型中,与游离CA相比,脂质体CA显着改善了肠道通透性,表明通过内吞摄取增强了生物利用度。这些结果表明,食品级卵磷脂与互补特性(如SLP-WHITE的静电稳定性和pl - 30s的膜刚性)的合理组合可以提高脂质体的物理稳定性和功能。该研究为开发适合食品应用的功能性脂质体载体提供了实用策略,特别是用于递送生物利用度较差的酚类化合物,如对香豆酸。
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引用次数: 0
Optimising Extraction Methods for Enhanced Lipid Recovery from Macroalgae. 大型藻脂质提取方法优化研究。
IF 1.8 4区 农林科学 Q3 CHEMISTRY, APPLIED Pub Date : 2026-01-01 DOI: 10.5650/jos.ess25136
Bhayu Gita Bhernama, Z A Muchlisin, Febriani, Teuku M Iqbalsyah

Biodiesel feedstocks have evolved from the use of food-grade vegetable oils to lipids from microalgae and microorganisms. The challenges faced in the production of biodiesel from this third-generation lipids lie in the efficient extraction techniques and production costs. This study aimed to optimise extraction techniques to enhance lipid recovery from macroalgae. Six macroalgal species from the northwest coast of Aceh, Indonesia, were initially screened to evaluate their lipid yields using the Soxhlet extraction (S) method. Caulerpa taxifolia was selected for further optimisation based on its high lipid yield and favourable fatty acid profile, consisting of 85.8 % palmitic acid (C16:0) and 14.2 % myristic acid (C14:0). Various extraction methods were tested, including ultrasonication (S), enzymatic extraction (E), and combinations of ultrasonication-Soxhlet extraction (US), enzymatic-ultrasonication (EU), enzymatic-Soxhlet extraction (ES), and enzymatic-ultrasonication-Soxhlet extraction (EUS). The ES method produced the highest lipid yield of 21.5 %, a 15-fold increase compared to that of the S method alone. Physicochemical analysis of the extracted lipids showed properties suitable for biodiesel production, although further refinement is required to fully meet the quality standards. This study demonstrates the potential of optimised extraction techniques to significantly enhance lipid recovery from macroalgae, thereby contributing to the development of sustainable marine-biofuel resources. Further research is required to refine these methods and explore their large-scale applicability in the field.

生物柴油的原料已经从食品级植物油发展到微藻和微生物的脂质。从第三代脂质生产生物柴油面临的挑战在于高效的提取技术和生产成本。本研究旨在优化提取工艺,提高巨藻脂质回收率。采用索氏提取法对印度尼西亚亚齐西北海岸的6种大型藻类进行了初步筛选,以评估其脂质产量。以紫杉树茎叶为原料,选择其高脂质产率和良好的脂肪酸结构,由85.8%的棕榈酸(C16:0)和14.2%的肉豆蔻酸(C14:0)组成。试验了超声法(S)、酶法(E)、超声-索氏提取法(US)、酶-索氏提取法(EU)、酶-索氏提取法(ES)、酶-超声-索氏提取法(EUS)等多种提取方法。ES法的脂质得率最高,为21.5%,比单独使用S法提高了15倍。对提取的脂质进行理化分析,结果表明其性质适合生产生物柴油,但仍需进一步提纯才能完全达到质量标准。这项研究证明了优化提取技术的潜力,可以显著提高大型藻类的脂质回收率,从而有助于可持续海洋生物燃料资源的开发。这些方法需要进一步的研究来完善,并探索其在该领域的大规模适用性。
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引用次数: 0
The Stability and Gastrointestinal Digestion Characteristics of Natural Walnut Oil Body Emulsion. 天然核桃油体乳的稳定性及胃肠消化特性研究。
IF 1.8 4区 农林科学 Q3 CHEMISTRY, APPLIED Pub Date : 2026-01-01 DOI: 10.5650/jos.ess25187
Shenghua He, Sanjiu Zhou, Yonghui Wang, Guanghui Li, Xueli Gao, Weiyun Guo

To understand the stability and gastrointestinal digestion characteristics of the natural walnut oil bodies (OBs) emulsion, walnut OBs were extracted from fresh walnuts and dispersed in aqueous phase to form O/W natural emulsions. The results showed that the natural walnut OBs emulsion droplets were uniformly dispersed in the aqueous phase as spherical particles, with triglycerides in the core and proteins surrounding them. SDS-PAGE indicated that the molecular weight of these proteins was below 55 kDa. The natural walnut OBs emulsion exhibited unstable at pH 4.0 and 6.0. Within the salt ion concentration range of 0-500 mM, the natural walnut OBs emulsion exhibited stable. Within the heat treatment temperature range of 0-85°C, the natural walnut OBs emulsion presented stable, but the droplets of the natural walnut OBs emulsion showed obvious aggregation at 95°C and became unstable. During the 0-120 min gastric digestion process, the droplets of the natural walnut OBs emulsion showed obvious aggregation and oil-water phases separation, and the particle size significantly increased with the extension of digestion time. During the 0-120 min small intestinal digestion stage, as the digestion time increased, the droplet size and the number of droplets decreased, while the release of free fatty acids continuously increased. This study lays a foundation for the application of walnut OBs in the food industry and the delivery of liposoluble bioactive substances.

为了解天然核桃油体乳状液的稳定性和胃肠道消化特性,从新鲜核桃中提取核桃油体,并在水相中分散形成O/W天然乳状液。结果表明:天然核桃OBs乳液滴在水相中呈球形均匀分散,其核心为甘油三酯,周围为蛋白质;SDS-PAGE分析表明,这些蛋白的分子量在55 kDa以下。天然核桃OBs乳液在pH为4.0和6.0时表现出不稳定性。在盐离子浓度0 ~ 500 mM范围内,天然核桃OBs乳状液表现稳定。在0 ~ 85℃的热处理温度范围内,天然核桃OBs乳状液呈现稳定状态,但在95℃时,天然核桃OBs乳状液液滴出现明显聚集,变得不稳定。在0 ~ 120 min胃消化过程中,天然核桃OBs乳状液液滴呈现明显的聚集和油水相分离,且随着消化时间的延长,粒径显著增大。在0 ~ 120 min小肠消化阶段,随着消化时间的增加,微滴大小和微滴数量减少,游离脂肪酸的释放量不断增加。本研究为核桃OBs在食品工业中的应用和脂溶性生物活性物质的输送奠定了基础。
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Journal of oleo science
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