Pub Date : 2024-10-22DOI: 10.1016/j.jpba.2024.116540
Xina Yu , Shanshan Song , Zhanhua Li , Tiantian Wang , Hui Huang , Qing Shen , Zongyuan Wu , Pei Luo
Panaxynol is a bioactive polyacetylene in food plants; however, its specific benefits in diabetes and metabolic disorders remain unclear. Previous studies have mainly focused on biochemical indicators and clinical evaluations. Limited research has systematically elucidated the beneficial effects of panaxynol from the oxylipins perspective. In this study, we employed an oxylipin analysis platform we previously established using high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) based on the multiple reaction monitoring (MRM) method for the profiling of oxylipins. After a 7-week administration of panaxynol to db/db mice, significant alterations in serum oxylipins and potential benefits to hyperglycemia, insulin resistance, and hepatic steatosis were observed. Our analysis also revealed correlations among epoxygenase products derived from arachidonic acid (AA), linoleic acid (LA), and α-linolenic acid (ALA) via cytochrome P450 (CYP) pathways. Furthermore, six potential oxylipins were identified, as offering insights into the mechanisms by which panaxynol may modulate diabetes. These results provide the first in vivo evidence of the impact of panaxynol on oxylipin metabolism and lay the foundation for developing panaxynol as a nutraceutical for diabetes management.
{"title":"Targeted LC-MS/MS method of oxylipin profiling reveals differentially expressed serum metabolites in type 2 diabetes mice with panaxynol","authors":"Xina Yu , Shanshan Song , Zhanhua Li , Tiantian Wang , Hui Huang , Qing Shen , Zongyuan Wu , Pei Luo","doi":"10.1016/j.jpba.2024.116540","DOIUrl":"10.1016/j.jpba.2024.116540","url":null,"abstract":"<div><div>Panaxynol is a bioactive polyacetylene in food plants; however, its specific benefits in diabetes and metabolic disorders remain unclear. Previous studies have mainly focused on biochemical indicators and clinical evaluations. Limited research has systematically elucidated the beneficial effects of panaxynol from the oxylipins perspective. In this study, we employed an oxylipin analysis platform we previously established using high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) based on the multiple reaction monitoring (MRM) method for the profiling of oxylipins. After a 7-week administration of panaxynol to db/db mice, significant alterations in serum oxylipins and potential benefits to hyperglycemia, insulin resistance, and hepatic steatosis were observed. Our analysis also revealed correlations among epoxygenase products derived from arachidonic acid (AA), linoleic acid (LA), and α-linolenic acid (ALA) via cytochrome P450 (CYP) pathways. Furthermore, six potential oxylipins were identified, as offering insights into the mechanisms by which panaxynol may modulate diabetes. These results provide the first <em>in vivo</em> evidence of the impact of panaxynol on oxylipin metabolism and lay the foundation for developing panaxynol as a nutraceutical for diabetes management.</div></div>","PeriodicalId":16685,"journal":{"name":"Journal of pharmaceutical and biomedical analysis","volume":"253 ","pages":"Article 116540"},"PeriodicalIF":3.1,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142546064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-22DOI: 10.1016/j.jpba.2024.116541
Baiyun Zhao , Jing Zhang , Kaiyue Zhao , Bin Wang , Jing Liu , Chaoxuan Wang , Ling Zeng , Xin Zeng , Yan Luo
Rapamycin (Rapa) is an inhibitor of mTOR complex, and its therapeutic effect on liver function was examined in non-alcoholic fatty liver disease (NAFLD) rats here. And the possible mechanism of Rapa in NAFLD was preliminarily elucidated based on the non-targeted metabolomics analysis. Adult male SD rats were fed with a high-fat and high-cholesterol diet (HFD) to establish NAFLD model. For Rapa group, 0.8 mg/(kg.d) Rapa was given to the HFD rats. Ultra-performance liquid chromatography and Q-Tof-mass spectrometry (UPLC and Q-TOF/MS) analysis were applied for the identification of metabolites in the serum of rats, which were annotated using Kyoto Encyclopedia of Genes and Genomes (KEGG). NAFLD rats presented with disturbed liver function, lipid metabolism and oxidative stress, but Rapa exerted a mitigating influence on the disorders. The metabolite profile data identified 579 metabolites that varied remarkably between the Rapa and HFD groups, with the main classes of amino acids and peptides, benzene, lipids and fatty acids. The differential metabolites were mainly involved in biosynthesis of cofactors, bile secretion, and glycerophospholipid metabolism were mainly enriched. In conclusion, Rapa has a potential protective effect against HFD-induced NAFLD, its hepatoprotective effect may achieved through mediating bile secretion and glycerophospholipid metabolism.
{"title":"Effect of rapamycin on hepatic metabolomics of non-alcoholic fatty liver rats based on non-targeted platform","authors":"Baiyun Zhao , Jing Zhang , Kaiyue Zhao , Bin Wang , Jing Liu , Chaoxuan Wang , Ling Zeng , Xin Zeng , Yan Luo","doi":"10.1016/j.jpba.2024.116541","DOIUrl":"10.1016/j.jpba.2024.116541","url":null,"abstract":"<div><div>Rapamycin (Rapa) is an inhibitor of mTOR complex, and its therapeutic effect on liver function was examined in non-alcoholic fatty liver disease (NAFLD) rats here. And the possible mechanism of Rapa in NAFLD was preliminarily elucidated based on the non-targeted metabolomics analysis. Adult male SD rats were fed with a high-fat and high-cholesterol diet (HFD) to establish NAFLD model. For Rapa group, 0.8 mg/(kg.d) Rapa was given to the HFD rats. Ultra-performance liquid chromatography and Q-Tof-mass spectrometry (UPLC and Q-TOF/MS) analysis were applied for the identification of metabolites in the serum of rats, which were annotated using Kyoto Encyclopedia of Genes and Genomes (KEGG). NAFLD rats presented with disturbed liver function, lipid metabolism and oxidative stress, but Rapa exerted a mitigating influence on the disorders. The metabolite profile data identified 579 metabolites that varied remarkably between the Rapa and HFD groups, with the main classes of amino acids and peptides, benzene, lipids and fatty acids. The differential metabolites were mainly involved in biosynthesis of cofactors, bile secretion, and glycerophospholipid metabolism were mainly enriched. In conclusion, Rapa has a potential protective effect against HFD-induced NAFLD, its hepatoprotective effect may achieved through mediating bile secretion and glycerophospholipid metabolism.</div></div>","PeriodicalId":16685,"journal":{"name":"Journal of pharmaceutical and biomedical analysis","volume":"253 ","pages":"Article 116541"},"PeriodicalIF":3.1,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142531589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-20DOI: 10.1016/j.jpba.2024.116539
Wenchang Fu , Kaige Yang , Mingyuan Wu , Yan Wang
The Mucin1 (MUC1) protein, involved in cytoprotective and signaling pathways, is abnormally elevated in various cancers, making it a key cancer indicator. Exosomes, which reflect the status of their originating cells, offer potential for cancer diagnosis. Thus, developing a method to detect MUC1-positive exosomes is crucial for the early diagnosis of certain cancers. In this study, we developed a highly sensitive, specific, and simple UV–visible signal amplification method to detect MUC1-positive exosomes using terminal deoxynucleotidyl transferase (TdT). Initially, exosomes were captured on magnetic beads using a CD63 aptamer(apt). The Primer-AuNPs-MUC1 apt complex which we synthesized by low pH loading method was then attached MUC1 proteins on the surface of the exosomes to create a sandwich structure. TdT catalyzed the extension of Biotin-dATP at the 3′ end of the primer, introducing multiple biotin sites into the sandwich structure. These sites subsequently bound multiple streptavidin-horseradish peroxidase (streptavidin-HRP), which catalyzed the oxidative color change of the substrate, which can be detected by colorimetric method. This method can detect A549 exosomes in the range of 1.4E+6 to 4.2E+8 particles/mL and shows high specificity for cell lines with different MUC1 expression. Additionally, it successfully distinguished cholangiocarcinoma (CCA) patients (n=11) from healthy individuals (n=7) in clinical serum assays, demonstrating good performance in real sample detection.
{"title":"Terminal deoxynucleotidyl transferase (TdT) based template-free signal amplification for the detection of exosomes in MUC1-positive cells","authors":"Wenchang Fu , Kaige Yang , Mingyuan Wu , Yan Wang","doi":"10.1016/j.jpba.2024.116539","DOIUrl":"10.1016/j.jpba.2024.116539","url":null,"abstract":"<div><div>The Mucin1 (MUC1) protein, involved in cytoprotective and signaling pathways, is abnormally elevated in various cancers, making it a key cancer indicator. Exosomes, which reflect the status of their originating cells, offer potential for cancer diagnosis. Thus, developing a method to detect MUC1-positive exosomes is crucial for the early diagnosis of certain cancers. In this study, we developed a highly sensitive, specific, and simple UV–visible signal amplification method to detect MUC1-positive exosomes using terminal deoxynucleotidyl transferase (TdT). Initially, exosomes were captured on magnetic beads using a CD63 aptamer(apt). The Primer-AuNPs-MUC1 apt complex which we synthesized by low pH loading method was then attached MUC1 proteins on the surface of the exosomes to create a sandwich structure. TdT catalyzed the extension of Biotin-dATP at the 3′ end of the primer, introducing multiple biotin sites into the sandwich structure. These sites subsequently bound multiple streptavidin-horseradish peroxidase (streptavidin-HRP), which catalyzed the oxidative color change of the substrate, which can be detected by colorimetric method. This method can detect A549 exosomes in the range of 1.4E+6 to 4.2E+8 particles/mL and shows high specificity for cell lines with different MUC1 expression. Additionally, it successfully distinguished cholangiocarcinoma (CCA) patients (n=11) from healthy individuals (n=7) in clinical serum assays, demonstrating good performance in real sample detection.</div></div>","PeriodicalId":16685,"journal":{"name":"Journal of pharmaceutical and biomedical analysis","volume":"253 ","pages":"Article 116539"},"PeriodicalIF":3.1,"publicationDate":"2024-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142502481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-20DOI: 10.1016/j.jpba.2024.116538
Yang Hu , Xin Jiang , Xiefeng Zhang , Yuxin Lan , Shaohui Cai , Taotao Xu , Xinyue Zhuang , Morili Asheng , Jing Zeng , Yongping Qin , Guangsheng Qian
L-Boronophenylalanine (BPA), a widely used 10B carrier for clinical boron neutron capture therapy (BNCT), was quantified in rat plasma through a simple, effective and stable ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method. Chromatographic separation was performed on an ACQUITY UPLC HSS T3 (100 mm × 2.1 mm, 1.8 μm) column with the mobile phase of 0.5 % formic acid aqueous solution and acetonitrile. For the detector, the m/z ion pairs used for quantification were 209.1→120.1 for BPA and 210.1→120.1 for internal standard in a positive mode by electrospray ionization (ESI) using multiple reaction monitoring (MRM). The method is specific and robust with rare affection by endogenous substances in the matrix. A good linear relationship was observed over 80–80000 ng/mL (r2 = 0.9993). The values of inter- and intra-day accuracy and precision were within the acceptance criteria of ±15 %. BPA was found to be stable under different experimental conditions. This developed method was successfully applied on a pharmacokinetic experiment on Sprague-Dawley rats (intravenous injection, 125 mg/kg) and a comparation between UHPLC-MS/MS and ICP-MS for BPA was carried out.
通过一种简单、有效和稳定的超高效液相色谱-串联质谱（UHPLC-MS/MS）方法对大鼠血浆中广泛应用于临床硼中子俘获疗法（BNCT）的10B载体--L-硼苯丙氨酸（BPA）进行了定量分析。色谱分离采用ACQUITY UPLC HSS T3色谱柱（100 mm × 2.1 mm, 1.8 μm），流动相为0.5%甲酸水溶液和乙腈。检测器为电喷雾离子化（ESI），采用多反应监测（MRM）正离子模式，双酚 A 的 m/z 离子对为 209.1→120.1，内标离子对为 210.1→120.1。该方法特异性强，稳定性好，很少受到基质中内源性物质的影响。在 80-80000 纳克/毫升的范围内观察到良好的线性关系（r2 = 0.9993）。日间和日内的准确度和精密度均在±15%的标准范围内。在不同的实验条件下，双酚 A 的稳定性良好。该方法成功地应用于Sprague-Dawley大鼠的药代动力学实验（静脉注射，125 mg/kg），并进行了UHPLC-MS/MS和ICP-MS测定双酚A的比较。
{"title":"A novel quantitative method for the determination of 10B-carrier boronophenylalanine in rat plasma by UHPLC-MS/MS and comparison with ICP-MS","authors":"Yang Hu , Xin Jiang , Xiefeng Zhang , Yuxin Lan , Shaohui Cai , Taotao Xu , Xinyue Zhuang , Morili Asheng , Jing Zeng , Yongping Qin , Guangsheng Qian","doi":"10.1016/j.jpba.2024.116538","DOIUrl":"10.1016/j.jpba.2024.116538","url":null,"abstract":"<div><div>L-Boronophenylalanine (BPA), a widely used <sup>10</sup>B carrier for clinical boron neutron capture therapy (BNCT), was quantified in rat plasma through a simple, effective and stable ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method. Chromatographic separation was performed on an ACQUITY UPLC HSS T3 (100 mm × 2.1 mm, 1.8 μm) column with the mobile phase of 0.5 % formic acid aqueous solution and acetonitrile. For the detector, the <em>m/z</em> ion pairs used for quantification were 209.1→120.1 for BPA and 210.1→120.1 for internal standard in a positive mode by electrospray ionization (ESI) using multiple reaction monitoring (MRM). The method is specific and robust with rare affection by endogenous substances in the matrix. A good linear relationship was observed over 80–80000 ng/mL (r<sup>2</sup> = 0.9993). The values of inter- and intra-day accuracy and precision were within the acceptance criteria of ±15 %. BPA was found to be stable under different experimental conditions. This developed method was successfully applied on a pharmacokinetic experiment on Sprague-Dawley rats (intravenous injection, 125 mg/kg) and a comparation between UHPLC-MS/MS and ICP-MS for BPA was carried out.</div></div>","PeriodicalId":16685,"journal":{"name":"Journal of pharmaceutical and biomedical analysis","volume":"253 ","pages":"Article 116538"},"PeriodicalIF":3.1,"publicationDate":"2024-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142502445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Strobilanthes sarcorrhiza, a folk medicine from China, is known to treat kidney deficiency and lumbago. However, its protective effects and mechanisms against diabetic nephropathy (DN) remain unclear. This study aimed to investigate the effects and mechanisms of Strobilanthes sarcorrhiza root phenolic extract (CTS) on streptozotocin (STZ)-induced DN in mice. Firstly, the constituents in CTS were characterized by UPLC-QTOF-MS. Thirty-three constituents were identified, including 12 phenylethanoid glycosides and their derivatives, 14 phenylpropanoid glycosides derivatives, 6 polyphenols derivatives, and 1 other constituent. Then, utilizing the identified constituents of CTS, network pharmacology was used to anticipate potential pathways against DN. Thirty-two out of thirty-three constituents showed anti-DN activity; their mechanism of action was significantly linked to tumor-, glycosylation-, metabolism-related pathways, etc. Furthermore, the effectiveness of CTS against DN and its in vivo mechanism was assessed by combining immunohistochemistry, untargeted metabolomics, biochemical evaluation, and histopathological examination. The findings showed that CTS improved blood glucose and lipid levels in diabetic mice, reduced serum levels of ALT, CREA, UREA, IL-1β, and IL-17, decreased pathological damage and fibrosis in kidney tissue, and lowered the protein expression of VEGF, Laminin, TNF-α, and NF-κB in kidney tissue. Metabolomics results indicated that CTS alleviated DN mainly by regulating glycerophospholipid metabolism. To the best of our knowledge, this study is the first to report that Strobilanthes sarcorrhiza attenuates DN, potentially through the inhibition of the NF-κB pathway, leading to a reduction in the inflammatory response and fibrosis of renal tissue. These findings suggest that Strobilanthes sarcorrhiza could be a promising therapeutic agent for DN.
{"title":"Strobilanthes sarcorrhiza root phenolic extract prevent diabetic nephropathy in mice by regulating NF-κB/IL-1β signaling and glycerophospholipid metabolism","authors":"Rongchang Chen , Jiaping Fan , Yiwei Wu , Xueli Huang , Wenting Zhang , Yuyan Xu , Yunhan Zhang , Longyu Li , Chaojie Wang , Meng Yu , Yindi Zhu","doi":"10.1016/j.jpba.2024.116534","DOIUrl":"10.1016/j.jpba.2024.116534","url":null,"abstract":"<div><div><em>Strobilanthes sarcorrhiza</em>, a folk medicine from China, is known to treat kidney deficiency and lumbago. However, its protective effects and mechanisms against diabetic nephropathy (DN) remain unclear. This study aimed to investigate the effects and mechanisms of <em>Strobilanthes sarcorrhiza</em> root phenolic extract (CTS) on streptozotocin (STZ)-induced DN in mice. Firstly, the constituents in CTS were characterized by UPLC-QTOF-MS. Thirty-three constituents were identified, including 12 phenylethanoid glycosides and their derivatives, 14 phenylpropanoid glycosides derivatives, 6 polyphenols derivatives, and 1 other constituent. Then, utilizing the identified constituents of CTS, network pharmacology was used to anticipate potential pathways against DN. Thirty-two out of thirty-three constituents showed anti-DN activity; their mechanism of action was significantly linked to tumor-, glycosylation-, metabolism-related pathways, etc. Furthermore, the effectiveness of CTS against DN and its <em>in vivo</em> mechanism was assessed by combining immunohistochemistry, untargeted metabolomics, biochemical evaluation, and histopathological examination. The findings showed that CTS improved blood glucose and lipid levels in diabetic mice, reduced serum levels of ALT, CREA, UREA, IL-1β, and IL-17, decreased pathological damage and fibrosis in kidney tissue, and lowered the protein expression of VEGF, Laminin, TNF-α, and NF-κB in kidney tissue. Metabolomics results indicated that CTS alleviated DN mainly by regulating glycerophospholipid metabolism. To the best of our knowledge, this study is the first to report that <em>Strobilanthes sarcorrhiza</em> attenuates DN, potentially through the inhibition of the NF-κB pathway, leading to a reduction in the inflammatory response and fibrosis of renal tissue. These findings suggest that <em>Strobilanthes sarcorrhiza</em> could be a promising therapeutic agent for DN.</div></div>","PeriodicalId":16685,"journal":{"name":"Journal of pharmaceutical and biomedical analysis","volume":"253 ","pages":"Article 116534"},"PeriodicalIF":3.1,"publicationDate":"2024-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142502479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-19DOI: 10.1016/j.jpba.2024.116537
Yan Cao , Xiaoxue Yang , Pengliang Shi, Guozhong Niu, Suzhen Zhang, Zhengwei Gu, Qingmei Guo
Moutan Cortex, is the root bark of Paeonia suffruticosa Andrews, which is classified into three specifications according to whether or not it is peeled and cored: Liandanpi, Guadanpi and whole root. In this study, the cork layer, cortex, phloem and xylem of P. suffruticosa fresh root were precisely separated by laser microdissection technique. UPLC-Q-Orbitrap-MS and UPLC-QQQ-MS techniques were used to analyse the differences in the chemical composition of different tissue parts of P. suffruticosa fresh root and Liandanpi, and to determine the optimal processing method of P. suffruticosa root. As a result, a total of 90 compounds were characterised, among which the cork layer had more types and higher contents of chemical constituents, and the xylem had fewer types and lower contents of chemical constituents. The proportion of xylem is larger, while the type and content of active ingredients is smaller. Therefore, the processing method of removing the wood core and retaining the cork bark can be used in the processing of Moutan Cortex. In this study, laser microdissection and ultra performance liquid chromatography-mass spectrometry were used to provide a theoretical basis for optimising the processing method of Moutan Cortex to enhance its pharmacological effects.
{"title":"Tissue-specific chemical expression and quantitative analysis of bioactive components of Moutan Cortex by laser-microdissection combined with UPLC-Q-Orbitrap-MS technique","authors":"Yan Cao , Xiaoxue Yang , Pengliang Shi, Guozhong Niu, Suzhen Zhang, Zhengwei Gu, Qingmei Guo","doi":"10.1016/j.jpba.2024.116537","DOIUrl":"10.1016/j.jpba.2024.116537","url":null,"abstract":"<div><div>Moutan Cortex, is the root bark of <em>Paeonia suffruticosa</em> Andrews, which is classified into three specifications according to whether or not it is peeled and cored: Liandanpi, Guadanpi and whole root. In this study, the cork layer, cortex, phloem and xylem of <em>P. suffruticosa</em> fresh root were precisely separated by laser microdissection technique. UPLC-Q-Orbitrap-MS and UPLC-QQQ-MS techniques were used to analyse the differences in the chemical composition of different tissue parts of <em>P. suffruticosa</em> fresh root and Liandanpi, and to determine the optimal processing method of <em>P. suffruticosa</em> root. As a result, a total of 90 compounds were characterised, among which the cork layer had more types and higher contents of chemical constituents, and the xylem had fewer types and lower contents of chemical constituents. The proportion of xylem is larger, while the type and content of active ingredients is smaller. Therefore, the processing method of removing the wood core and retaining the cork bark can be used in the processing of Moutan Cortex. In this study, laser microdissection and ultra performance liquid chromatography-mass spectrometry were used to provide a theoretical basis for optimising the processing method of Moutan Cortex to enhance its pharmacological effects.</div></div>","PeriodicalId":16685,"journal":{"name":"Journal of pharmaceutical and biomedical analysis","volume":"253 ","pages":"Article 116537"},"PeriodicalIF":3.1,"publicationDate":"2024-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142502482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-19DOI: 10.1016/j.jpba.2024.116513
Jingru Song , Yanping Huang , Lu Liu , Dengcheng Hui , Zheng Wang , Dong Xie , Yulang Jiang , Hongyan Cao , Yancheng Dai , Guan Ye , Shibing Su , Mingmei Zhou , Qin Zhang , Mingyu Sun
Yinchenhao decoction (YCHD) is widely used in the treatment of damp-heat syndrome of chronic hepatitis B (CHB), but it remains unclear about the active compounds in YCHD and its potential mechanism for treating CHB. The purpose of this work is to evaluate the clinical efficacy of YCHD combined with nucleoside analogues (NAs) for the treatment of CHB. Besides, based on the exact clinical efficacy, we combined serum metabolomics and network pharmacology to screen differential metabolites and related pathways regulated by YCHD to investigate the possible mechanism for treating CHB. It revealed that NAs plus YCHD could significantly improve alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels, increase HBV-DNA negative rate (P<0.05), reduce the levels of inflammatory factors and LSM (both P<0.05), regulate lipids (P<0.05), and improve the symptoms of traditional Chinese medicine (TCM) (P<0.05) in CHB patients. YCHD was relatively safe. It showed 30 active compounds including chlorogenic acid, geniposide, emodin, quercetin, kaempferol, β-sitosterol and aloe emodin, and 115 key targets which were related to the regulation of lipids and reduction of oxidative stress related to the effect of YCHD in CHB in the network pharmacology analysis. We found 9 core targets and 4 key metabolites according to metabolomics, which were partly consistent with the network pharmacology findings. It proved that network pharmacology combined with metabolomics can well explain the “multi-component-multi-target” mechanism of complex TCM.
{"title":"Integrated metabolomics and network pharmacology to explore the clinical efficacy and mechanism of Yinchenhao decoction combined with nucleoside analogues on chronic hepatitis B","authors":"Jingru Song , Yanping Huang , Lu Liu , Dengcheng Hui , Zheng Wang , Dong Xie , Yulang Jiang , Hongyan Cao , Yancheng Dai , Guan Ye , Shibing Su , Mingmei Zhou , Qin Zhang , Mingyu Sun","doi":"10.1016/j.jpba.2024.116513","DOIUrl":"10.1016/j.jpba.2024.116513","url":null,"abstract":"<div><div>Yinchenhao decoction (YCHD) is widely used in the treatment of damp-heat syndrome of chronic hepatitis B (CHB), but it remains unclear about the active compounds in YCHD and its potential mechanism for treating CHB. The purpose of this work is to evaluate the clinical efficacy of YCHD combined with nucleoside analogues (NAs) for the treatment of CHB. Besides, based on the exact clinical efficacy, we combined serum metabolomics and network pharmacology to screen differential metabolites and related pathways regulated by YCHD to investigate the possible mechanism for treating CHB. It revealed that NAs plus YCHD could significantly improve alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels, increase HBV-DNA negative rate (<em>P</em><0.05), reduce the levels of inflammatory factors and LSM (both <em>P</em><0.05), regulate lipids (<em>P</em><0.05), and improve the symptoms of traditional Chinese medicine (TCM) (<em>P</em><0.05) in CHB patients. YCHD was relatively safe. It showed 30 active compounds including chlorogenic acid, geniposide, emodin, quercetin, kaempferol, β-sitosterol and aloe emodin, and 115 key targets which were related to the regulation of lipids and reduction of oxidative stress related to the effect of YCHD in CHB in the network pharmacology analysis. We found 9 core targets and 4 key metabolites according to metabolomics, which were partly consistent with the network pharmacology findings. It proved that network pharmacology combined with metabolomics can well explain the “multi-component-multi-target” mechanism of complex TCM.</div></div>","PeriodicalId":16685,"journal":{"name":"Journal of pharmaceutical and biomedical analysis","volume":"253 ","pages":"Article 116513"},"PeriodicalIF":3.1,"publicationDate":"2024-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142502449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-18DOI: 10.1016/j.jpba.2024.116535
Jaime Millán-Santiago, Carlos Calero-Cañuelo, Rafael Lucena, Soledad Cárdenas
Direct coupling sample preparation with mass spectrometry has risen as a reliable analytical strategy in bioanalysis as it provides a high sample throughput. This approach avoids an exhaustive separation step, thus being cost-effective compared to the traditional analytical workflow. The selectivity and sensitivity levels rely on the mass spectrometric analysis and the appropriate selection of the sample preparation. Miniaturized extraction techniques have demonstrated particular utility in this coupling thanks to their ability to pre-concentrate the target analytes while removing many of the matrix components. This article reviews the main developments in combining microextraction techniques with mass spectrometry based on electrospray ionization, a consolidated ionization technique in bioanalysis. The article aims to provide an overview of the potential of these techniques by describing the most significant examples. The different approaches are classified according to the materials or devices used to perform the extraction and analysis.
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Pub Date : 2024-10-18DOI: 10.1016/j.jpba.2024.116529
Yanni Xue , Changling Lv , Lu Jin , Di Tan , Dingyu Wu , Fang Peng
Xinmailong (XML), a traditional Chinese medicine derived from Periplaneta americana, is commonly used in China to treat chronic heart failure (CHF). However, its pharmacological mechanism remains unclear. In our research, we employed Doxorubicin (Dox) to create a CHF animal model and administered XML treatment to investigate the pharmacological effects of XML on CHF rats by combining transcriptomic and proteomic analyses. XML improved dox-induced CHF and improved cardiac function, and a joint multi-omics analysis demonstrated that it reduced cardiomyocyte fibrosis during CHF. There is further evidence that XML may alleviate cardiomyocyte fibrosis through its effects on the cGMP-PKG signaling pathway or by reducing the expression levels of COL1A1, COL3A1, MMP9, and CXCR2. In this study, the effects of XML on rats with CHF are examined at the transcriptional and protein levels, as well as its mechanism and mode of action in treating CHF. There may be novel therapeutic targets or clinical indications for XML-based CHF therapy resulting from the study's identification of significant differential genes and signaling pathways.
新麦隆（Xinmailong，XML）是一种从美洲长春花（Periplaneta americana）中提取的传统中药，在中国常用于治疗慢性心力衰竭（CHF）。然而，其药理机制仍不清楚。在我们的研究中，我们利用多柔比星（Dox）建立了一个CHF动物模型，并给予XML治疗，通过结合转录组学和蛋白质组学分析，研究XML对CHF大鼠的药理作用。XML改善了Dox诱导的CHF并改善了心脏功能，一项多组学联合分析表明，XML减少了CHF期间的心肌细胞纤维化。有进一步的证据表明，XML 可通过影响 cGMP-PKG 信号通路或降低 COL1A1、COL3A1、MMP9 和 CXCR2 的表达水平来减轻心肌细胞纤维化。本研究从转录和蛋白质水平研究了 XML 对慢性心力衰竭大鼠的影响及其治疗慢性心力衰竭的机制和作用模式。通过本研究发现的重要差异基因和信号通路，基于 XML 的慢性心力衰竭疗法可能会有新的治疗靶点或临床适应症。
{"title":"Study on the mechanism of Xinmailong injection against chronic heart failure based on transcriptomics and proteomics","authors":"Yanni Xue , Changling Lv , Lu Jin , Di Tan , Dingyu Wu , Fang Peng","doi":"10.1016/j.jpba.2024.116529","DOIUrl":"10.1016/j.jpba.2024.116529","url":null,"abstract":"<div><div>Xinmailong (XML), a traditional Chinese medicine derived from Periplaneta americana, is commonly used in China to treat chronic heart failure (CHF). However, its pharmacological mechanism remains unclear. In our research, we employed Doxorubicin (Dox) to create a CHF animal model and administered XML treatment to investigate the pharmacological effects of XML on CHF rats by combining transcriptomic and proteomic analyses. XML improved dox-induced CHF and improved cardiac function, and a joint multi-omics analysis demonstrated that it reduced cardiomyocyte fibrosis during CHF. There is further evidence that XML may alleviate cardiomyocyte fibrosis through its effects on the cGMP-PKG signaling pathway or by reducing the expression levels of COL1A1, COL3A1, MMP9, and CXCR2. In this study, the effects of XML on rats with CHF are examined at the transcriptional and protein levels, as well as its mechanism and mode of action in treating CHF. There may be novel therapeutic targets or clinical indications for XML-based CHF therapy resulting from the study's identification of significant differential genes and signaling pathways.</div></div>","PeriodicalId":16685,"journal":{"name":"Journal of pharmaceutical and biomedical analysis","volume":"253 ","pages":"Article 116529"},"PeriodicalIF":3.1,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142502480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-18DOI: 10.1016/j.jpba.2024.116536
Abdelaziz Moutcine , Charaf Laghlimi , Younes Ziat , Soumia El Bahraoui , Hamza Belkhanchi , Ahmed Jouaiti
This study reviews advances in chemical detection methods applied to the metabolic products known as uric acid (UA) and xanthine (XA), which are residues of purine metabolism, with XA being an important intermediate preceding UA. UA and XA play crucial roles in maintaining physiological homeostasis in organisms. Chemical modification of electrodes is a widely used method to address the issues of poor sensitivity and selectivity encountered with bare electrodes. This article reviews various materials commonly used to modify electrode surfaces for the detection of uric acid and xanthine, focusing on properties that enhance electrocatalytic activity. We highlight recent trends in detecting these compounds using electrochemical methods with microfabricated devices and explore cutting-edge modification techniques involving novel nanomaterials, carbon derivatives, metallic nanoparticles, and polymers. The review includes a comparative analysis of these materials, addressing their strengths, limitations, and recent advancements, such as in carbon-based materials and metal-organic frameworks (MOFs). Finally, we critically examine the challenges and future prospects of electrochemical detection of UA and XA in real samples, offering strategies to address these issues.
The challenges associated with determination of UA and XA in real samples are also discussed.
尿酸（UA）和黄嘌呤（XA）是嘌呤代谢的残留物，其中 XA 是 UA 的重要中间产物。UA 和 XA 在维持生物体内生理平衡方面发挥着至关重要的作用。为解决裸电极灵敏度和选择性差的问题，对电极进行化学修饰是一种广泛使用的方法。本文回顾了常用于修饰电极表面以检测尿酸和黄嘌呤的各种材料，重点介绍了可提高电催化活性的特性。我们重点介绍了使用微加工设备的电化学方法检测这些化合物的最新趋势，并探讨了涉及新型纳米材料、碳衍生物、金属纳米颗粒和聚合物的前沿改性技术。综述包括对这些材料的比较分析，探讨它们的优势、局限性和最新进展，如碳基材料和金属有机框架（MOFs）。最后，我们对实际样品中 UA 和 XA 的电化学检测所面临的挑战和未来前景进行了认真研究，并提出了解决这些问题的策略。我们还讨论了在实际样品中测定 UA 和 XA 所面临的挑战。
{"title":"Advanced design of chemically modified electrodes for the electrochemical analysis of uric acid and xanthine","authors":"Abdelaziz Moutcine , Charaf Laghlimi , Younes Ziat , Soumia El Bahraoui , Hamza Belkhanchi , Ahmed Jouaiti","doi":"10.1016/j.jpba.2024.116536","DOIUrl":"10.1016/j.jpba.2024.116536","url":null,"abstract":"<div><div>This study reviews advances in chemical detection methods applied to the metabolic products known as uric acid (UA) and xanthine (XA), which are residues of purine metabolism, with XA being an important intermediate preceding UA. UA and XA play crucial roles in maintaining physiological homeostasis in organisms. Chemical modification of electrodes is a widely used method to address the issues of poor sensitivity and selectivity encountered with bare electrodes. This article reviews various materials commonly used to modify electrode surfaces for the detection of uric acid and xanthine, focusing on properties that enhance electrocatalytic activity. We highlight recent trends in detecting these compounds using electrochemical methods with microfabricated devices and explore cutting-edge modification techniques involving novel nanomaterials, carbon derivatives, metallic nanoparticles, and polymers. The review includes a comparative analysis of these materials, addressing their strengths, limitations, and recent advancements, such as in carbon-based materials and metal-organic frameworks (MOFs). Finally, we critically examine the challenges and future prospects of electrochemical detection of UA and XA in real samples, offering strategies to address these issues.</div><div>The challenges associated with determination of UA and XA in real samples are also discussed.</div></div>","PeriodicalId":16685,"journal":{"name":"Journal of pharmaceutical and biomedical analysis","volume":"253 ","pages":"Article 116536"},"PeriodicalIF":3.1,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142546062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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