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Chemical profiles of essential oils of two cultivars of Melaleuca cajuputi leaves and flowers Melaleuca cajuputi 两个栽培品种的叶和花的精油化学成分概况
Q4 Agricultural and Biological Sciences Pub Date : 2024-03-14 DOI: 10.25081/jp.2024.v16.8007
Hong Thia Le, Nguyen Tuong An Huynh
In the present study, the differences between the chemical compositions of the essential oils obtained from the leaves and flowers of two cultivars of M. cajuputi collected from Moc Hoa district, Long An province. By using Gas Chromatography-Mass Spectrometry, a total of 105 components have been identified in the essential oils of four samples of two M. cajuputi cultivars such as “Tràm gió” leaves, “Tràm gió” flowers, “Tràm cừ” leaves and “Tràm cừ” flowers. The Agglomerative Hierarchical Cluster (AHC) and Principal Component Analysis (PCA) were performed to show the similarities/dissimilarities in chemical compositions among the four studied samples. As a result, the components of the essential oils of four studied samples were divided into two clusters. Cluster I included two samples such as “Tràm gió” leaf and “Tràm gió” flower with high presence of 1,8-cineole (35.12 and 17.69%), linalool (3.31 and 5.03%), (R)-α-terpinyl acetate (9.17 and 8.1%). Cluster II comprised “Tràm cừ” leaf and “Tràm cừ” flower with the high concentration of α-pinene (9.87 and 12.19%), γ-terpinene (10.48 and 11.3%), p-mentha-2,4(8)-diene (8.8 and 12.7%).
本研究探讨了从隆安省莫华县采集的两种 M. cajuputi 栽培品种的叶和花中提取的精油化学成分之间的差异。通过气相色谱-质谱分析法,我们鉴定出两种 M. cajuputi 栽培品种的四种精油中的 105 种成分,如 "Tràm gió "叶、"Tràm gió "花、"Tràm cừ "叶和 "Tràm cừ "花。聚类分析(AHC)和主成分分析(PCA)显示了四种研究样本化学成分的相似性和不相似性。结果,四个研究样本的精油成分被分为两组。聚类 I 包括两个样本,如 "Tràm gió "叶和 "Tràm gió "花,其中 1,8-蒎烯(35.12% 和 17.69%)、芳樟醇(3.31% 和 5.03%)、(R)-α-松油醇乙酸酯(9.17% 和 8.1%)含量较高。第 II 组包括 "Tràm cừ "叶和 "Tràm cừ "花,α-蒎烯(9.87 和 12.19%)、γ-松油醇(10.48 和 11.3%)、对薄荷-2,4(8)-二烯(8.8 和 12.7%)含量较高。
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引用次数: 0
Combination of composted poultry manure and inorganic fertilizers enhance growth and yield of tomato (Lycopersicon esculentum Mill.) in a rooftop growing system 将堆肥家禽粪便和无机肥结合使用,提高屋顶种植系统中番茄(Lycopersicon esculentum Mill.)的生长和产量
Q4 Agricultural and Biological Sciences Pub Date : 2024-03-10 DOI: 10.25081/jp.2024.v16.8717
Moomtahina Rahman, Md. Alauddin, G. M. Mohsin, Md. Amirul Alam, M. K. Rahman
Rooftop vegetable productions are becoming an important part of the recent rejuvenation of urban agriculture particularly in densely populated urban areas. However, due to weight limits often associated with rooftop growing systems, rooftop plant growing media including soil, may not contain optimal fertility levels required to maximize plant growth and productivity. Therefore, the success of rooftop vegetable production often depends on proper fertility management schemes to create optimal plant growing conditions. Therefore, an experiment was conducted to investigate combine impacts of composted poultry manure (CPM) and inorganic fertilizers on growth and yield of tomato under rooftop growing conditions. The experiment was arranged in a completely randomized design (CRD) with eight treatments and three replications. The treatments included T1: Control (no CPM and no NPK), T2: 4 ton CPM ha-1, T3: N55P15K50 kg ha-1, T4: N110P30K100 kg ha-1, T5: N165P45K150 kg ha-1, T6: 4 ton CPM ha-1+ N55P15K50 kg ha-1, T7: 4 ton CPM ha-1+ N110P30K100 kg ha-1, and T8: 4 ton CPM ha-1+N165P45K150 kg ha-1. The results showed that NPK fertilizers alone and CPM combined with NPK fertilizers greatly improved tomato plant growth and fruit yield. However, maximum plant growth and tomato yields (68 t ha-1) and economic benefits (benefit: cost ratio 6.9) were achieved with 4 t ha-1 CPM amendment and 100% recommended doses of NPK fertilizers. Control treatment (T1: -CPM, -NPK) had the lowest tomato yield (6.2 t ha-1). Composted poultry manure alone contributed for around 15.0 t ha-1 tomato yield and supplemented for around 30 kg ha-1 N fertilizer. Combine application of 4 t ha-1 CPM and 100% RDF of NPK fertilizers indicated as the agreeable combination in this study for optimal tomato plant growth and yield under rooftop growing conditions.
屋顶蔬菜生产正在成为近年来城市农业复兴的重要组成部分,尤其是在人口稠密的城市地区。然而,由于屋顶种植系统通常存在重量限制,包括土壤在内的屋顶植物生长介质可能无法提供植物生长和产量最大化所需的最佳肥力水平。因此,屋顶蔬菜生产的成功往往取决于适当的肥力管理方案,以创造最佳的植物生长条件。因此,我们进行了一项实验,研究堆肥家禽粪便(CPM)和无机肥料对屋顶种植条件下番茄生长和产量的综合影响。实验采用完全随机设计(CRD),共设 8 个处理和 3 次重复。处理包括 T1:对照(不施用 CPM 和 NPK),T2:4 吨 CPM ha-1,T3:N55P15K50 kg ha-1,T4:T5: N165P45K150 kg ha-1,T6: 4 吨 CPM ha-1+N55P15K50 kg ha-1,T7: 4 吨 CPM ha-1+N110P30K100 kg ha-1,T8: 4 吨 CPM ha-1+N165P45K150 kg ha-1。结果表明,单独施用氮磷钾肥料和 CPM 与氮磷钾肥料结合施用可大大提高番茄植株的生长和果实产量。然而,在施用 4 吨/公顷 CPM 和 100%推荐剂量的氮磷钾肥时,植株生长和番茄产量(68 吨/公顷)以及经济效益(效益成本比 6.9)均达到最大值。对照处理(T1:-CPM、-NPK)的番茄产量最低(6.2 吨/公顷-1)。单独施用堆肥的番茄产量约为 15.0 吨/公顷,补充氮肥约为 30 千克/公顷。在这项研究中,结合施用 4 吨/公顷 CPM 和 100% RDF 的氮磷钾肥料是屋顶种植条件下番茄植株生长和产量最佳的组合。
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引用次数: 0
Exploring genetic diversity and trait associations with foliar blast disease among parental lines in pearl millet [Pennisetum glaucum (L.) R Br] 探索珍珠粟[Pennisetum glaucum (L.) R Br]亲本品系的遗传多样性及其与叶瘟病的性状相关性
Q4 Agricultural and Biological Sciences Pub Date : 2024-03-05 DOI: 10.25081/jp.2024.v16.8797
R. Rasitha, R. Kalaiyarasi, K. Iyanar, N. Senthil, I. Johnson
Thirty-seven pearl millet genotypes were evaluated for morphometric traits and disease incidence and severity during summer and kharif, 2022. Pooled ANOVA revealed significant variation were present in all genotypes across different season. Association studies identified high positive correlations between grain yield and traits such as single earhead weight, single ear head threshed weight, and test weight, with direct and indirect effects on grain yield through key characters. E2 (kharif – 2022) showed favourable conducive weather parameters for disease infection throughout the growing season compared to E1. The higher PCV relative to GCV for disease incidence underscores the environmental influence in disease resistance programs. Negative correlations between disease metrics and yield traits highlight blast disease’s detrimental effect on grain yield. Disease severity indirectly suggests environmental factors may enhance its impact. Disease incidence exhibited a direct negative impact on yield, supported by negative genotypic correlations. The line, PT 6679, exhibit both high yield and highly resistant to blast. Restorer lines (PT 6029, PT 6067, PT 6300, PT 6707, PT 7068) and B lines (ICMB 01666, ICMB 02777) showed promising yield attributes with high to moderate disease resistant for future breeding programs. In D2 analysis, five clusters revealed distinct genetic diversity with Clusters II and V indicating strong hybrid vigor, while Clusters IV (PT 6946, ICMB 06111) and V (ICMB 93111, ICMB 95444) excelled in disease resistance. Clusters I (PT 6029, PT 7068) and II (GMR 58) exhibited superior grain yield, particularly Cluster I, had potential restorer lines for future breeding. Clear differentiation between B and R lines underscored genetic distinctions in trait expression, validating the use of morphological data for assessing genetic diversity.
在 2022 年夏季和秋季,对 37 个珍珠粟基因型的形态特征、病害发生率和严重程度进行了评估。汇总方差分析显示,所有基因型在不同季节都存在显著差异。关联研究发现,谷物产量与单穗重、单穗脱粒重和测试重等性状之间存在高度正相关,主要性状对谷物产量有直接或间接的影响。与 E1 相比,E2(喀里多尼亚-2022 年)在整个生长季节都表现出有利于病害感染的天气参数。病害发生率的 PCV 相对于 GCV 较高,凸显了环境对抗病计划的影响。病害指标与产量性状之间的负相关突出表明了稻瘟病对谷物产量的不利影响。病害严重程度间接表明环境因素可能会增强其影响。病害发生率对产量有直接的负面影响,并得到基因型负相关的支持。PT 6679 这个品系既高产又高度抗病。恢复系(PT 6029、PT 6067、PT 6300、PT 6707、PT 7068)和 B 系(ICMB 01666、ICMB 02777)表现出高至中等抗病的产量属性,有望用于未来的育种计划。在 D2 分析中,五个聚类显示出明显的遗传多样性,聚类 II 和聚类 V 显示出较强的杂交活力,而聚类 IV(PT 6946、ICMB 06111)和聚类 V(ICMB 93111、ICMB 95444)则在抗病性方面表现突出。第 I 组(PT 6029、PT 7068)和第 II 组(GMR 58)表现出优异的谷物产量,尤其是第 I 组,有可能成为未来育种的恢复系。B 品系和 R 品系之间的明显区分突出了性状表达的遗传差异,验证了利用形态学数据评估遗传多样性的有效性。
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引用次数: 0
Phytochemical analysis of root extracts of Rhynchostylis retusa (L.) Blume from the Eastern Ghats of India 印度东高止山Rhynchostylis retusa (L.) Blume根提取物的植物化学分析
Q4 Agricultural and Biological Sciences Pub Date : 2024-02-14 DOI: 10.25081/jp.2024.v16.8755
Shaik Noorjahan, M. Rahamtulla, S. M. khasim
Rhynchostylis retusa (Orchidaceae) is an important ethnomedicinal herb in Indian systems of medicine. Tribal healers commonly employ the roots of this epiphytic orchid to treat various ailments. In the present study, the shade-dried root powder of R. retusa was subjected to cold extraction with four solvents, such as n-hexane, acetone, ethyl acetate, and methanol. The crude root extracts were then taken for qualitative phytochemical screening. Furthermore, GC-MS analysis of ethyl acetate and methanol root extracts was carried out. The methanol root extract of R. retusa showed a positive result for all the phytochemicals tested except for saponins. GC-MS analysis of R. retusa methanol root extract revealed the presence of 16 phytoconstituents. Major phytoconstituents such as 9-Hexadecenoic acid, methyl ester, [Z] Heptadecanoic acid, 16-methyl-, methyl ester, Ethanone,1-[4-(4-morpholyl benzylidene amino)phenyl]-, are recorded in the methanol root extract. In the present study, both methanol and ethyl acetate root extracts showed the presence of9-Hexadecenoic acid, methyl ester, [Z] as the major phytocompound. The phytocompounds identified in methanol and ethyl acetate root extracts exhibit various biological activities, including anti-inflammatory, antibacterial, antifungal, antioxidant, and anticancer properties. Based on the findings of the current study, it can be inferred that the roots of R. retusa contain diverse bioactive compounds with medicinal properties. Further investigation of these R. retusa root extracts holds the potential for the discovery and development of innovative pharmaceuticals.
在印度医学体系中,Rhynchostylis retusa(兰科)是一种重要的民族药用植物。部落医士通常使用这种附生兰花的根来治疗各种疾病。本研究采用正己烷、丙酮、乙酸乙酯和甲醇等四种溶剂对阴干的 R. retusa 根粉进行冷萃取。然后对粗根提取物进行定性植物化学筛选。此外,还对乙酸乙酯和甲醇根提取物进行了气相色谱-质谱分析。除了皂苷外,R. retusa 的甲醇根提取物对所有测试的植物化学物质都显示出阳性结果。R. retusa 甲醇根提取物的气相色谱-质谱分析表明,其中含有 16 种植物成分。甲醇根提取物中记录的主要植物成分有:9-十六烯酸甲酯、[Z] 十七烷酸 16-甲基甲酯、乙酮、1-[4-(4-吗啉基苯亚氨基)苯基]-。在本研究中,甲醇和乙酸乙酯根提取物均显示出 9-十六碳烯酸甲酯[Z]是主要的植物化合物。甲醇和乙酸乙酯根提取物中鉴定出的植物化合物具有多种生物活性,包括抗炎、抗菌、抗真菌、抗氧化和抗癌特性。根据目前的研究结果,可以推断出网纹草根含有多种具有药用价值的生物活性化合物。对这些 R. retusa 根提取物的进一步研究有望发现和开发创新药物。
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引用次数: 0
Phytochemical analysis of root extracts of Rhynchostylis retusa (L.) Blume from the Eastern Ghats of India 印度东高止山Rhynchostylis retusa (L.) Blume根提取物的植物化学分析
Q4 Agricultural and Biological Sciences Pub Date : 2024-02-14 DOI: 10.25081/jp.2024.v16.8755
Shaik Noorjahan, M. Rahamtulla, S. M. khasim
Rhynchostylis retusa (Orchidaceae) is an important ethnomedicinal herb in Indian systems of medicine. Tribal healers commonly employ the roots of this epiphytic orchid to treat various ailments. In the present study, the shade-dried root powder of R. retusa was subjected to cold extraction with four solvents, such as n-hexane, acetone, ethyl acetate, and methanol. The crude root extracts were then taken for qualitative phytochemical screening. Furthermore, GC-MS analysis of ethyl acetate and methanol root extracts was carried out. The methanol root extract of R. retusa showed a positive result for all the phytochemicals tested except for saponins. GC-MS analysis of R. retusa methanol root extract revealed the presence of 16 phytoconstituents. Major phytoconstituents such as 9-Hexadecenoic acid, methyl ester, [Z] Heptadecanoic acid, 16-methyl-, methyl ester, Ethanone,1-[4-(4-morpholyl benzylidene amino)phenyl]-, are recorded in the methanol root extract. In the present study, both methanol and ethyl acetate root extracts showed the presence of9-Hexadecenoic acid, methyl ester, [Z] as the major phytocompound. The phytocompounds identified in methanol and ethyl acetate root extracts exhibit various biological activities, including anti-inflammatory, antibacterial, antifungal, antioxidant, and anticancer properties. Based on the findings of the current study, it can be inferred that the roots of R. retusa contain diverse bioactive compounds with medicinal properties. Further investigation of these R. retusa root extracts holds the potential for the discovery and development of innovative pharmaceuticals.
在印度医学体系中,Rhynchostylis retusa(兰科)是一种重要的民族药用植物。部落医士通常使用这种附生兰花的根来治疗各种疾病。本研究采用正己烷、丙酮、乙酸乙酯和甲醇等四种溶剂对阴干的 R. retusa 根粉进行冷萃取。然后对粗根提取物进行定性植物化学筛选。此外,还对乙酸乙酯和甲醇根提取物进行了气相色谱-质谱分析。除了皂苷外,R. retusa 的甲醇根提取物对所有测试的植物化学物质都显示出阳性结果。R. retusa 甲醇根提取物的气相色谱-质谱分析表明,其中含有 16 种植物成分。甲醇根提取物中记录的主要植物成分有:9-十六烯酸甲酯、[Z] 十七烷酸 16-甲基甲酯、乙酮、1-[4-(4-吗啉基苯亚氨基)苯基]-。在本研究中,甲醇和乙酸乙酯根提取物均显示出 9-十六碳烯酸甲酯[Z]是主要的植物化合物。甲醇和乙酸乙酯根提取物中鉴定出的植物化合物具有多种生物活性,包括抗炎、抗菌、抗真菌、抗氧化和抗癌特性。根据目前的研究结果,可以推断出网纹草根含有多种具有药用价值的生物活性化合物。对这些 R. retusa 根提取物的进一步研究有望发现和开发创新药物。
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引用次数: 0
Influence of medium and gelling agents concentration on in vitro rooting of Polygonum tinctorium 培养基和胶凝剂浓度对何首乌离体生根的影响
Q4 Agricultural and Biological Sciences Pub Date : 2024-02-07 DOI: 10.25081/jp.2024.v16.8793
Minsol Choi, Haeng-Hoon Kim, Hyewon Seo, R. Sathasivam, Jae Kwang Kim, Sang Un Park
Polygonum tinctorium has long been employed in the medical and dye industries in many nations, particularly in Korea and Japan, for traditional fabric staining as a source of blue color. The plant tissue culture method has proven to be a rapid and sustainable approach for the regeneration of various plant species, particularly those with medicinal or ornamental value. In this study, we explored the in vitro root regeneration and growth of P. tinctorium in response to different growth media and gelling agents. Among the tested media, Schenk and Hildebrandt medium (SH medium) demonstrated superior performance, resulting in the highest number and length of roots per explant. SH media produced about 53 and 18% higher root per explant and a 40% and 14% increase in root length compared to B5 and MS media, respectively. Half strength of SH medium proved to be the optimal condition for both root number (7.46 roots per explant) and root length (3.81 mm). Among the gelling agents, 2 g/L of Gelrite medium was most effective in promoting the highest and tallest roots. These findings have the potential to enhance rooting abilities in various crops, particularly in the case of medicinal and ornamental plants, and may offer valuable insights for future industrial-scale root production of P. tinctorium.
许多国家,尤其是韩国和日本,长期以来一直将何首乌用于医药和染料行业,作为传统织物染色的蓝色来源。植物组织培养方法已被证明是一种快速、可持续的再生方法,适用于多种植物物种,尤其是具有药用或观赏价值的植物物种。在这项研究中,我们探讨了锡兰的体外根再生和生长对不同生长介质和胶凝剂的反应。在测试的培养基中,Schenk 和 Hildebrandt 培养基(SH 培养基)表现优异,每个外植体生根的数量和长度都最高。与 B5 和 MS 培养基相比,SH 培养基每个外植体产生的根分别增加了 53% 和 18%,根的长度分别增加了 40% 和 14%。事实证明,半强度的 SH 培养基是根数量(每外植体 7.46 根)和根长度(3.81 毫米)的最佳条件。在胶凝剂中,2 克/升的 Gelrite 培养基对促进生出最多和最高的根最为有效。这些发现有可能提高各种作物的生根能力,尤其是药用和观赏植物的生根能力,并可能为未来工业化大规模生产洋二仙草的根提供宝贵的见解。
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引用次数: 0
Influence of medium and gelling agents concentration on in vitro rooting of Polygonum tinctorium 培养基和胶凝剂浓度对何首乌离体生根的影响
Q4 Agricultural and Biological Sciences Pub Date : 2024-02-07 DOI: 10.25081/jp.2024.v16.8793
Minsol Choi, Haeng-Hoon Kim, Hyewon Seo, R. Sathasivam, Jae Kwang Kim, Sang Un Park
Polygonum tinctorium has long been employed in the medical and dye industries in many nations, particularly in Korea and Japan, for traditional fabric staining as a source of blue color. The plant tissue culture method has proven to be a rapid and sustainable approach for the regeneration of various plant species, particularly those with medicinal or ornamental value. In this study, we explored the in vitro root regeneration and growth of P. tinctorium in response to different growth media and gelling agents. Among the tested media, Schenk and Hildebrandt medium (SH medium) demonstrated superior performance, resulting in the highest number and length of roots per explant. SH media produced about 53 and 18% higher root per explant and a 40% and 14% increase in root length compared to B5 and MS media, respectively. Half strength of SH medium proved to be the optimal condition for both root number (7.46 roots per explant) and root length (3.81 mm). Among the gelling agents, 2 g/L of Gelrite medium was most effective in promoting the highest and tallest roots. These findings have the potential to enhance rooting abilities in various crops, particularly in the case of medicinal and ornamental plants, and may offer valuable insights for future industrial-scale root production of P. tinctorium.
许多国家,尤其是韩国和日本,长期以来一直将何首乌用于医药和染料行业,作为传统织物染色的蓝色来源。植物组织培养方法已被证明是一种快速、可持续的再生方法,适用于多种植物物种,尤其是具有药用或观赏价值的植物物种。在这项研究中,我们探讨了锡兰的体外根再生和生长对不同生长介质和胶凝剂的反应。在测试的培养基中,Schenk 和 Hildebrandt 培养基(SH 培养基)表现优异,每个外植体生根的数量和长度都最高。与 B5 和 MS 培养基相比,SH 培养基每个外植体产生的根分别增加了 53% 和 18%,根的长度分别增加了 40% 和 14%。事实证明,半强度的 SH 培养基是根数量(每外植体 7.46 根)和根长度(3.81 毫米)的最佳条件。在胶凝剂中,2 克/升的 Gelrite 培养基对促进生出最多和最高的根最为有效。这些发现有可能提高各种作物的生根能力,尤其是药用和观赏植物的生根能力,并可能为未来工业化大规模生产洋二仙草的根提供宝贵的见解。
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引用次数: 0
The influence of elevated CO2 concentrations and UVB radiation in antioxidant activity of selected Chenopodium quinoa varieties 高浓度二氧化碳和紫外线辐射对部分藜麦品种抗氧化活性的影响
Q4 Agricultural and Biological Sciences Pub Date : 2023-12-20 DOI: 10.25081/jp.2023.v15.8738
Saif Ali Matar Al Blooshi, Nasser Abdullah Ghdayer Al Kaabi, Karthishwaran Kandhan, Abdul Jaleel, Mohammed Alyafei
Ecosystems have been affected by climate change. Both agriculture and environmental changes are correlated with various features since climate change is the main cause of abiotic and biotic stress, which affects crop plants. Climate change and its severe impact on plant productivity showed great intensity due to the effects of abiotic stress. In the present investigation, we selected two quinoa varieties to study the response to future climatic factors such as eCO2, enhanced UVB radiation, and UVB+eCO2 combined effects in open-top chambers in the hot climate of the UAE. The treatments were administered for 90 days in the hot UAE weather conditions and the experiment was carried out in a transparent OTC facility. The response of the studied quinoa varieties was measured by analyzing their non-enzymatic antioxidant and antioxidant enzyme activities. Our findings showed that quinoa varieties are suitable as industrial crops for their levels of antioxidants under stimulating climatic conditions because the quantity and quality of their yield have not been affected. Based on the results obtained in the present investigation, further study is warranted for screening more varieties with the addition of climate change factors such as temperature and humidity to find more tolerant varieties of quinoa suitable for future climatic conditions.
生态系统受到气候变化的影响。农业和环境变化都与各种特征相关,因为气候变化是造成非生物和生物胁迫的主要原因,而非生物和生物胁迫会影响农作物。由于非生物胁迫的影响,气候变化及其对植物生产力的严重影响表现得非常强烈。在本次调查中,我们选择了两个藜麦品种,在阿联酋炎热气候下的敞篷室中研究其对未来气候因素(如二氧化碳、增强的紫外线辐射和紫外线+二氧化碳联合效应)的响应。在阿联酋炎热的天气条件下进行了为期 90 天的处理,实验在透明的 OTC 设施中进行。所研究的藜麦品种的反应是通过分析其非酶抗氧化活性和抗氧化酶活性来衡量的。我们的研究结果表明,在刺激性气候条件下,藜麦品种的抗氧化剂水平适合作为工业作物,因为它们的产量和质量没有受到影响。根据本次调查的结果,我们有必要进一步研究,在增加温度和湿度等气候变化因素的情况下筛选更多品种,以找到更多适合未来气候条件的耐受性更强的藜麦品种。
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引用次数: 0
Nutritional composition and antioxidant capacity of Urtica hyperborea: A phytofood of Trans-Himalayan region of Ladakh, India 荨麻的营养成分和抗氧化能力:印度拉达克跨喜马拉雅地区的一种植物食品
Q4 Agricultural and Biological Sciences Pub Date : 2023-12-07 DOI: 10.25081/jp.2023.v15.8607
Disket Zomba, M. Dar, Rupali Jandrotia, Mansi Sharma, Rishikesh Singh, S. Kaur, D. Batish
Urtica hyperborea Jacquem. ex Wedd., a perennial plant of Urticaceae family is considered as a wild vegetable in the mountainous region of Ladakh. Due to its application in many forms of traditional culinary in every household during the harsh winter season, the plant ensures the food security. However, the nutritional composition and phytochemical analysis of U. hyperborea responsible for these beneficial features have not been explored widely. The present study aims to determine the nutritional composition (e.g., macromolecules, pigments, minerals, phenolics and flavonoid contents), antioxidant activity and the phytochemical analysis of this plant species present in Ladakh, India. The radical scavenging and antioxidant potential of the plant were evaluated by assays like 2,2‒diphenyl‒1‒picrylhydrazyl (DPPH), hydrogen peroxide (H2O2), hydroxyl (ºOH), and ferric ion reducing antioxidant power (FRAP) for different extracts prepared in water, methanol, ethyl acetate, and petroleum ether. Macromolecules such as protein, carbohydrate, total phenolic and flavonoid contents in U. hyperborea were found to be 62.28±6.67, 170.80±3.98, 24.47±0.39 and 5.43±0.97 mg g-1, respectively. Similarly, dried powder of U. hyperborea was found to be rich in different mineral contents such as potassium, magnesium, sodium, manganese, zinc and iron. Among the various solvents used for exploring scavenging and antioxidant potential, aqueous extracts showed highest activity with 79.2% in DPPH assay as compared to other extracts. Similar trend was observed for other assays where aqueous extracts exhibited higher activity followed by methanolic, ethyl acetate and petroleum ether extracts. Significant positive linear correlations were observed between the radical scavenging/antioxidant activity of aqueous extracts and their content of phenolic/flavonoid compounds. The identification of phenolic compounds such as coumarin, quercetin, and ferulic acid confirm the antioxidative nature of the plant. Overall, rich macromolecule and mineral contents, as well as higher radical scavenging/antioxidant activities in aqueous extracts of U. hyperborea revealed that the plant has significant potential to be utilized as a phytofood source in harsh environmental conditions.
雅可姆的荨麻疹。Wedd交货。是一种荨麻科多年生植物,被认为是拉达克山区的一种野生蔬菜。由于在严酷的冬季,它以多种形式应用于每个家庭的传统烹饪,确保了粮食安全。然而,对这些有益特性的营养成分和植物化学分析尚未得到广泛的探讨。本研究旨在确定印度拉达克地区这种植物的营养成分(如大分子、色素、矿物质、酚类物质和类黄酮含量)、抗氧化活性和植物化学分析。通过对水、甲醇、乙酸乙酯和石油醚制备的不同提取物进行DPPH、过氧化氢、羟基和铁离子还原抗氧化能力(FRAP)的测定,评价了该植物的自由基清除能力和抗氧化能力。大分子蛋白质、碳水化合物、总酚和类黄酮含量分别为62.28±6.67、170.80±3.98、24.47±0.39和5.43±0.97 mg g-1。同样地,人们发现木犀草的干粉富含不同的矿物质,如钾、镁、钠、锰、锌和铁。在各种溶剂中,水提液的DPPH活性最高,为79.2%。类似的趋势也被观察到其他测定,其中水提取物表现出较高的活性,其次是甲醇,乙酸乙酯和石油醚提取物。水提物的自由基清除/抗氧化活性与其酚类/类黄酮含量呈显著的线性正相关。酚类化合物如香豆素、槲皮素和阿魏酸的鉴定证实了该植物的抗氧化性质。总之,北方木犀草水提取物中丰富的大分子和矿物质含量,以及较高的自由基清除/抗氧化活性表明,该植物具有在恶劣环境条件下作为植物食物来源的巨大潜力。
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引用次数: 0
Entomotoxicity of ZnO NPs synthesized using Clausena anisata Hook.f. ex Benth(ulmaayii) leaf extract against maize weevil, Sitophilus zeamais Mostch (Coleoptera: Curculionidae) 使用 Clausena anisata Hook.f. ex Benth(ulmaayii) 叶提取物合成的氧化锌纳米粒子对玉米象鼻虫(鞘翅目:虫科)的昆虫毒性研究
Q4 Agricultural and Biological Sciences Pub Date : 2023-12-04 DOI: 10.25081/jp.2023.v15.8620
Adugna Gindaba, M. Negeri, Abel Saka
The application of leaf extract to synthesize nanoparticles has been taken as a green method. In this study, the potential for synthesizing zinc oxide nanoparticles (ZnO NPs) from Clausena anisata Hook.f. ex Benth. leaf extract was investigated. The source of zinc was zinc nitrate hexahydrate (Zn(NO3)2.6H2O). The characterization study was done by Ultraviolet–visible (UV-Vis) spectroscopy, X-ray diffraction (XRD), scanning electron microscope (SEM), Transmission electron microscope (TEM) and atomic force microscopy (AFM). The crystalline shape of nanoparticles is disclosed inside the XRD result, morphology is confirmed through SEM effects, and consequently, the ZnO NPs scale was predicted. ZnO NPs were synthesized to work against Sitophilus zeamais adults. A mortality count was carried out in 14 days and all the 3 dosages (0.2 g, 0.4 g and 0.6 g) were effective in killing S. zeamais. F1 progeny emergence was highly reduced in comparison to untreated control. Maize seeds were successfully germinated after treatment application with ZnO NPs.
利用叶提取物合成纳米颗粒被认为是一种绿色的方法。在本研究中,研究了以山葵为原料合成氧化锌纳米粒子(ZnO NPs)的潜力。Benth交货。对叶提取物进行了研究。锌的来源为六水硝酸锌(Zn(NO3)2.6H2O)。采用紫外可见光谱(UV-Vis)、x射线衍射(XRD)、扫描电镜(SEM)、透射电镜(TEM)和原子力显微镜(AFM)对其进行了表征。XRD结果揭示了纳米颗粒的晶体形状,SEM结果证实了纳米颗粒的形貌,并对ZnO纳米粒子的尺度进行了预测。合成了氧化锌NPs对玉米象成虫的杀伤作用。14 d后进行死亡计数,0.2 g、0.4 g和0.6 g 3种剂量均能有效杀灭玉米玉米蚜。与未经处理的对照相比,F1后代的羽化程度大大降低。施氧化锌NPs处理后,玉米种子成功发芽。
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引用次数: 0
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Journal of Phytology
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