Pub Date : 2023-07-25DOI: 10.25081/jp.2023.v15.7914
Md. Amirul Alam, Md. Alauddin, Moomtahina Rahman, Md. Sajedur Rahman Rahman, G. M. Mohsin, M. Rahman
Rooftop farming is gaining rapid popularity in urban areas, especially since the beginning of the global COVID-19 pandemic. For housebound people rooftop farming is not only a way of potentially management of their time but also the execution of creativity. For rooftop farming vermicompost (VC) can be the most sustainable media for growing high value fruits and vegetables crops. In this regard, an experiment was carried out as a rooftop farming system at Charfassion upzila in the Bhola district of Bangladesh to observe the growth and yield performance of capsicum (Capsicum annuum) grown in different combinations of vermicompost in the winter season (2020-2021). Different agronomic and yield parameters were measured at the intervals of 30, 60, and 90 days and at the final harvest of 120 days after sowing. Among the measured parameters a mixed type of performance was achieved from varied doses of vermicompost applications and inorganic fertilizer treatment. Among the measured valuable parameters; the highest fruit length (8.85 cm), fruit diameter (10.3 cm), no. of fruits (9.51 plant-1), total wt. of fresh fruits (405.32 g/plant), fresh fruits yield (11.26 t ha-1), no. of branches (30.0 plant-1), stem girth (6.3 cm), root fresh wt. (8.80 g/plant), stem fresh wt. (23.35 g/plant), petiole fresh wt. (6.09 g/plant), total fresh biomass (65.54 g/plant), dry wt. of fruits (76.91 g/plant), dry wt. of total fruits: biomass ratio (7.35) and benefit-cost ratio (12.40), respectively, were observed in T5 (20t VC ha-1). So, from the overall findings of this study T5 with 20t VC application ha-1 is recommended to achieve better growth and yield of capsicum through the sustainable way of rooftop and urban farming systems and to improve soil fertility status.
{"title":"Vermicompost induced growth and yield performance of capsicum (Capsicum annuum L.) at sustainable rooftop farming system","authors":"Md. Amirul Alam, Md. Alauddin, Moomtahina Rahman, Md. Sajedur Rahman Rahman, G. M. Mohsin, M. Rahman","doi":"10.25081/jp.2023.v15.7914","DOIUrl":"https://doi.org/10.25081/jp.2023.v15.7914","url":null,"abstract":"Rooftop farming is gaining rapid popularity in urban areas, especially since the beginning of the global COVID-19 pandemic. For housebound people rooftop farming is not only a way of potentially management of their time but also the execution of creativity. For rooftop farming vermicompost (VC) can be the most sustainable media for growing high value fruits and vegetables crops. In this regard, an experiment was carried out as a rooftop farming system at Charfassion upzila in the Bhola district of Bangladesh to observe the growth and yield performance of capsicum (Capsicum annuum) grown in different combinations of vermicompost in the winter season (2020-2021). Different agronomic and yield parameters were measured at the intervals of 30, 60, and 90 days and at the final harvest of 120 days after sowing. Among the measured parameters a mixed type of performance was achieved from varied doses of vermicompost applications and inorganic fertilizer treatment. Among the measured valuable parameters; the highest fruit length (8.85 cm), fruit diameter (10.3 cm), no. of fruits (9.51 plant-1), total wt. of fresh fruits (405.32 g/plant), fresh fruits yield (11.26 t ha-1), no. of branches (30.0 plant-1), stem girth (6.3 cm), root fresh wt. (8.80 g/plant), stem fresh wt. (23.35 g/plant), petiole fresh wt. (6.09 g/plant), total fresh biomass (65.54 g/plant), dry wt. of fruits (76.91 g/plant), dry wt. of total fruits: biomass ratio (7.35) and benefit-cost ratio (12.40), respectively, were observed in T5 (20t VC ha-1). So, from the overall findings of this study T5 with 20t VC application ha-1 is recommended to achieve better growth and yield of capsicum through the sustainable way of rooftop and urban farming systems and to improve soil fertility status.","PeriodicalId":16777,"journal":{"name":"Journal of Phytology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48650303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-19DOI: 10.25081/jp.2023.v15.8366
R. Kambale, G. Ramasamy, Rajagopal Balasubramanian, S. Thiruvenkatasamy, V. Sekar
Doubled haploid (DH) technology remarkably accelerates the crop breeding by obtaining homozygous lines in a single generation. The present study was targeted in generating haploid plants through androgenesis. Anthers from immature flower buds of six tomato genotypes viz., LE-1230, LE-1236, LE-1256 TLCV 2, PKM 1 and TNAU tomato hybrid CO 3 were used for induction of haploids. A preliminary study based on callus induction frequency (CIF), more than 5% was helpful in short listing flower bud size, pre-treatments and growth regulator combinations. Subsequently, anthers from two different sized flower buds (4 and 6 mm length), dissected either from fresh or pre-treated flower buds (2 and 5 days in dark at 4 °C or gamma irradiated) were inoculated in MS medium fortified with different growth regulators for callus induction. Among the genotypes, TLCV 2 had recorded the maximum CIF (38.80%) from anthers of 4 mm long flower buds followed by TNAU tomato hybrid CO 3 (34.70%). Throughout the study, anthers from 4 mm long flower buds responded the best for callus induction. Among the pre-treatments, anthers from gamma irradiated flower buds recorded the highest CIF (31.90%) when compared to others. Cold shock (4 °C) in dark to flower buds for 2 days had improved the CIF of anthers when compared to fresh in LE 1230, LE 1238, TLCV2 and TNAU tomato hybrid CO 3, but when the cold shock was increased to 5 days, invariably there was a reduction in CIF in all the six genotypes. TA 8 (MS + 2iP (0.5 mg L-1) + NAA (0.5 mg L-1)) medium was found to be the best for maximum CIF in LE 1230 and PKM1, TA1 (MS + 2iP (1.0 mg L-1) + IAA (2.0 mg L-1)) in LE 1238, LE 1256 and TNAU tomato hybrid CO 3 and TA7 (MS + 2iP (0.5 mg L-1) + Kinetin (1.5 mg L-1) + NAA (1.0 mg L-1)) for TLCV 2 genotypes. The callus induced was sub cultured at monthly intervals in the same medium for proliferation and later transferred to regeneration medium. A good number of shoots got regenerated only from anther calli of TNAU hybrid CO 3 that was sub cultured in MS medium fortified with Zeatin (0.5 mg L-1). The clumps of shoots induced were separated and inoculated in MS medium supplemented with GA3 (0.5 mg L-1) for shoot elongation. After 4-6 weeks, the elongated shoots were transferred to half strength MS medium enhanced with IBA (1 mg L-1). Profuse rooting from the base of the shoot was noticed in 4-5 weeks. The stomatal count with leaves from the diploid plants and in vitro plants observed were 3-4 and 1 respectively indicating the haploidy nature of in vitro plants.
{"title":"Androgenesis in tomato (Solanum lycopersicum L.)-Effect of genotypes, microspore development stage, pre-treatments and media composition on induction of haploids","authors":"R. Kambale, G. Ramasamy, Rajagopal Balasubramanian, S. Thiruvenkatasamy, V. Sekar","doi":"10.25081/jp.2023.v15.8366","DOIUrl":"https://doi.org/10.25081/jp.2023.v15.8366","url":null,"abstract":"Doubled haploid (DH) technology remarkably accelerates the crop breeding by obtaining homozygous lines in a single generation. The present study was targeted in generating haploid plants through androgenesis. Anthers from immature flower buds of six tomato genotypes viz., LE-1230, LE-1236, LE-1256 TLCV 2, PKM 1 and TNAU tomato hybrid CO 3 were used for induction of haploids. A preliminary study based on callus induction frequency (CIF), more than 5% was helpful in short listing flower bud size, pre-treatments and growth regulator combinations. Subsequently, anthers from two different sized flower buds (4 and 6 mm length), dissected either from fresh or pre-treated flower buds (2 and 5 days in dark at 4 °C or gamma irradiated) were inoculated in MS medium fortified with different growth regulators for callus induction. Among the genotypes, TLCV 2 had recorded the maximum CIF (38.80%) from anthers of 4 mm long flower buds followed by TNAU tomato hybrid CO 3 (34.70%). Throughout the study, anthers from 4 mm long flower buds responded the best for callus induction. Among the pre-treatments, anthers from gamma irradiated flower buds recorded the highest CIF (31.90%) when compared to others. Cold shock (4 °C) in dark to flower buds for 2 days had improved the CIF of anthers when compared to fresh in LE 1230, LE 1238, TLCV2 and TNAU tomato hybrid CO 3, but when the cold shock was increased to 5 days, invariably there was a reduction in CIF in all the six genotypes. TA 8 (MS + 2iP (0.5 mg L-1) + NAA (0.5 mg L-1)) medium was found to be the best for maximum CIF in LE 1230 and PKM1, TA1 (MS + 2iP (1.0 mg L-1) + IAA (2.0 mg L-1)) in LE 1238, LE 1256 and TNAU tomato hybrid CO 3 and TA7 (MS + 2iP (0.5 mg L-1) + Kinetin (1.5 mg L-1) + NAA (1.0 mg L-1)) for TLCV 2 genotypes. The callus induced was sub cultured at monthly intervals in the same medium for proliferation and later transferred to regeneration medium. A good number of shoots got regenerated only from anther calli of TNAU hybrid CO 3 that was sub cultured in MS medium fortified with Zeatin (0.5 mg L-1). The clumps of shoots induced were separated and inoculated in MS medium supplemented with GA3 (0.5 mg L-1) for shoot elongation. After 4-6 weeks, the elongated shoots were transferred to half strength MS medium enhanced with IBA (1 mg L-1). Profuse rooting from the base of the shoot was noticed in 4-5 weeks. The stomatal count with leaves from the diploid plants and in vitro plants observed were 3-4 and 1 respectively indicating the haploidy nature of in vitro plants.","PeriodicalId":16777,"journal":{"name":"Journal of Phytology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44953171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-27DOI: 10.25081/jp.2023.v15.8387
Elycée Tindano, Benjamin Lankoandé, Stefan Porembski, Adjima Thiombiano
Faced with the loss of biodiversity, particularly plant diversity, due to anthropogenic pressure, particular ecosystems such as inselbergs can constitute refuge areas. The objective of this study is to determine the conservation potential of plant biodiversity on inselbergs in Burkina Faso. A comparative study was carried out between inselberg’s vegetation and the surrounding plains vegetation. In each of these vegetation types, stratified and random sampling was adopted and data were collected in plots of 900 m² for the woody stratum and 100 m² for the herbaceous stratum. The specific diversity was evaluated through the effective numbers of Hill of order Q = 0; 1; 2. The Hill index does not vary between inselbergs and plains and thus reflects a strong similarity in floristic diversity between the two ecosystems. Sorensen’s similarity coefficient also shows a similarity in terms of floristic composition between inselbergs and surrounding plains. In inselbergs, relict species that have disappeared from the surrounding plains of some phytogeographic sectors occur. The flora of inselbergs is also characterised by indicative species of anthropized ecosystem absence. However, these species are present on the surrounding plains. Indeed, the flora of inselbergs is characterised by 11 endemic species. Inselberg’s vegetation is characterised by unique plant communities such as rock pools and Afrotrilepis pilosa mats. The exploitation and mortality rates of woody plants are significantly higher on the surrounding plains than on the inselbergs. The flora and vegetation of inselbergs show the absence of indicator species of disturbed ecosystems. They present endemic and relict species and stable woody stands. Due to the steep slope, lack of suitable soil for agriculture and sacred status of someones, the inselbergs constitute a refuge for plant species and thus contribute to the conservation of biodiversity like the protected areas.
{"title":"Inselbergs: potential conservation areas for plant diversity in the face of anthropization","authors":"Elycée Tindano, Benjamin Lankoandé, Stefan Porembski, Adjima Thiombiano","doi":"10.25081/jp.2023.v15.8387","DOIUrl":"https://doi.org/10.25081/jp.2023.v15.8387","url":null,"abstract":"Faced with the loss of biodiversity, particularly plant diversity, due to anthropogenic pressure, particular ecosystems such as inselbergs can constitute refuge areas. The objective of this study is to determine the conservation potential of plant biodiversity on inselbergs in Burkina Faso. A comparative study was carried out between inselberg’s vegetation and the surrounding plains vegetation. In each of these vegetation types, stratified and random sampling was adopted and data were collected in plots of 900 m² for the woody stratum and 100 m² for the herbaceous stratum. The specific diversity was evaluated through the effective numbers of Hill of order Q = 0; 1; 2. The Hill index does not vary between inselbergs and plains and thus reflects a strong similarity in floristic diversity between the two ecosystems. Sorensen’s similarity coefficient also shows a similarity in terms of floristic composition between inselbergs and surrounding plains. In inselbergs, relict species that have disappeared from the surrounding plains of some phytogeographic sectors occur. The flora of inselbergs is also characterised by indicative species of anthropized ecosystem absence. However, these species are present on the surrounding plains. Indeed, the flora of inselbergs is characterised by 11 endemic species. Inselberg’s vegetation is characterised by unique plant communities such as rock pools and Afrotrilepis pilosa mats. The exploitation and mortality rates of woody plants are significantly higher on the surrounding plains than on the inselbergs. The flora and vegetation of inselbergs show the absence of indicator species of disturbed ecosystems. They present endemic and relict species and stable woody stands. Due to the steep slope, lack of suitable soil for agriculture and sacred status of someones, the inselbergs constitute a refuge for plant species and thus contribute to the conservation of biodiversity like the protected areas.","PeriodicalId":16777,"journal":{"name":"Journal of Phytology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135950867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-23DOI: 10.25081/jp.2023.v15.7397
Israt Yasmin, Sourov Adhikary, L. Hassan, G. Sagor
Rice blast caused by Magnoporthe oryzae is a major devastating fungal disease and represents a potential threat to world rice productions. However, information about the genetic and biochemical basis of disease tolerance is still limited. In this study, we tested the presence and diversity of resistant R genes using SSR markers, and the antioxidant enzymes catalase (CAT), ascorbate peroxidise (APX) and guaicol peroxidise (POD), activity and also the concentration of hydrogen peroxide (H2O2) and malondialdehyde (MDA) in resistant (BAUdhan 3) and susceptible (BRRIdhan 28) genotype. Molecular marker analysis reveals the presence of all ten studied resistant genes in BAUdhan 3. Among the markers studied, three markers namely RM224, RM72 and RM206 produce distinct band only in resistant genotype BAUdhan 3, which might be used to screen resistant genotypes. The enzymatic activity of APX, CAT and POD increased in the inoculated plant for both cultivars but the increase was more prominent for BAUdhan 3. The M. oryzae infections significantly increased the H2O2 content in BRRIdhan 28 and not much changed in BAUdhan 3. The MDA concentration was higher in the leaves of inoculated plants of BRRIdhan 28. The higher activities of APX and POD in the leaves of the inoculated plants of BAUdhan 3 resulted in lower H2O2 accumulation which can minimize the cellular damages possibly caused by reactive oxygen species. The result shows that the presence of more resistance genes and an effective antioxidative system in BAUdhan 3, which limits the damage caused due to fungal infection and thus contributes to greater resistance.
{"title":"Biochemical changes of rice genotypes against blast (Magnaporthe oryzae) disease and SSR marker validation for resistance genes","authors":"Israt Yasmin, Sourov Adhikary, L. Hassan, G. Sagor","doi":"10.25081/jp.2023.v15.7397","DOIUrl":"https://doi.org/10.25081/jp.2023.v15.7397","url":null,"abstract":"Rice blast caused by Magnoporthe oryzae is a major devastating fungal disease and represents a potential threat to world rice productions. However, information about the genetic and biochemical basis of disease tolerance is still limited. In this study, we tested the presence and diversity of resistant R genes using SSR markers, and the antioxidant enzymes catalase (CAT), ascorbate peroxidise (APX) and guaicol peroxidise (POD), activity and also the concentration of hydrogen peroxide (H2O2) and malondialdehyde (MDA) in resistant (BAUdhan 3) and susceptible (BRRIdhan 28) genotype. Molecular marker analysis reveals the presence of all ten studied resistant genes in BAUdhan 3. Among the markers studied, three markers namely RM224, RM72 and RM206 produce distinct band only in resistant genotype BAUdhan 3, which might be used to screen resistant genotypes. The enzymatic activity of APX, CAT and POD increased in the inoculated plant for both cultivars but the increase was more prominent for BAUdhan 3. The M. oryzae infections significantly increased the H2O2 content in BRRIdhan 28 and not much changed in BAUdhan 3. The MDA concentration was higher in the leaves of inoculated plants of BRRIdhan 28. The higher activities of APX and POD in the leaves of the inoculated plants of BAUdhan 3 resulted in lower H2O2 accumulation which can minimize the cellular damages possibly caused by reactive oxygen species. The result shows that the presence of more resistance genes and an effective antioxidative system in BAUdhan 3, which limits the damage caused due to fungal infection and thus contributes to greater resistance.","PeriodicalId":16777,"journal":{"name":"Journal of Phytology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42533504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-18DOI: 10.25081/jp.2023.v15.7742
Y. Othman, Tala S A’saf, Malik G. Al-Ajlouni, Muayyad Bany Hani, R. Hilaire
We assessed the influence of postharvest pulsing solutions pH and composition on cut flower quality of rose (Rosa hybrida cvs. Avalanche and Black Magic), gerbera (Gerbera jamesonii cv. Beaudine) and lily (Lilium × elegans cv. Fangio) under room (20±2 °C) and cold storage (4±1 °C) conditions. Cut flowers were placed in different acidic (pH, 3.5 - 4) or basic (pH, 7.0 - 7.5) preservative solutions containing water, sugar 5% (flower food), 100 mg/L silver nitrate (AgNO3 act as a bactericide), or a commercial product (2% sugar + bactericide and fungicide). Acidic solutions had higher or similar (never lower) vase life at both room and cold storage conditions and across species. In addition, vase life was 3-4 times longer in cold storage when compared to room conditions. Leaf chlorophyll concentrations for rose and lily were inconsistent or not significant across the species at both conditions (room and cold storage). The commercial preservative solution consistently and significantly had higher vase life than water for all tested cut flower species and under both room and cold storage environments. AgNO3 ranked second in terms of vase life enhancement. Overall, the use of only flower food (sugar) or bactericide (AgNO3) had a positive impact on vase life but only the combined use of a preservative substance (specifically at pH, 3.5 - 4.0) consistently guaranteed a high cut flower quality across flower species.
在室温（20±2°C）和冷藏（4±1°C）条件下，我们评估了采后脉冲溶液pH和成分对玫瑰（Rosa hybrida cvs.Avalanche and Black Magic）、非洲菊（gerbera jamesoii cv.Beaudine）和百合（Lilium×elegans cv.Fangio）切花质量的影响。将切花置于不同的酸性（pH，3.5-4）或碱性（pH，7.0-7.5）防腐剂溶液中，该溶液含有水、5%糖（花卉食品）、100mg/L硝酸银（AgNO3作为杀菌剂）或商业产品（2%糖+杀菌剂和杀菌剂）。酸性溶液在室温和冷藏条件下以及在不同物种之间具有更高或相似（从未降低）的花瓶寿命。此外，与室温条件相比，花瓶在冷藏中的寿命延长了3-4倍。在两种条件下（室温和冷藏），玫瑰和百合的叶片叶绿素浓度不一致或不显著。对于所有测试的切花品种，在室温和冷藏环境下，商业防腐剂溶液的花瓶寿命始终显著高于水。AgNO3在提高花瓶寿命方面排名第二。总体而言，仅使用花卉食品（糖）或杀菌剂（AgNO3）对花瓶寿命有积极影响，但只有联合使用防腐剂（特别是在pH值为3.5-4.0的条件下）才能保证所有花卉品种的高切花质量。
{"title":"Holding solution pH and composition consistently improve vase life of rose, lily and gerbera","authors":"Y. Othman, Tala S A’saf, Malik G. Al-Ajlouni, Muayyad Bany Hani, R. Hilaire","doi":"10.25081/jp.2023.v15.7742","DOIUrl":"https://doi.org/10.25081/jp.2023.v15.7742","url":null,"abstract":"We assessed the influence of postharvest pulsing solutions pH and composition on cut flower quality of rose (Rosa hybrida cvs. Avalanche and Black Magic), gerbera (Gerbera jamesonii cv. Beaudine) and lily (Lilium × elegans cv. Fangio) under room (20±2 °C) and cold storage (4±1 °C) conditions. Cut flowers were placed in different acidic (pH, 3.5 - 4) or basic (pH, 7.0 - 7.5) preservative solutions containing water, sugar 5% (flower food), 100 mg/L silver nitrate (AgNO3 act as a bactericide), or a commercial product (2% sugar + bactericide and fungicide). Acidic solutions had higher or similar (never lower) vase life at both room and cold storage conditions and across species. In addition, vase life was 3-4 times longer in cold storage when compared to room conditions. Leaf chlorophyll concentrations for rose and lily were inconsistent or not significant across the species at both conditions (room and cold storage). The commercial preservative solution consistently and significantly had higher vase life than water for all tested cut flower species and under both room and cold storage environments. AgNO3 ranked second in terms of vase life enhancement. Overall, the use of only flower food (sugar) or bactericide (AgNO3) had a positive impact on vase life but only the combined use of a preservative substance (specifically at pH, 3.5 - 4.0) consistently guaranteed a high cut flower quality across flower species.","PeriodicalId":16777,"journal":{"name":"Journal of Phytology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44581867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-20DOI: 10.25081/jp.2023.v15.8319
Young Chang Kim, W. Park, R. Sathasivam, H. Kim, J. K. Kim, Sang-Un Park
Liriope platyphylla can be multiplied either by planting seeds or dividing its tuberous roots. In this study, a method for L. platyphylla plant shoot organogenesis from meristem explants was developed employing medium and gelling agents. For the selection of optimal medium conditions for shoot organogenesis, the effects of full- and half-strength B5, SH, and MS media were examined. Different concentrations of the gelling agents such as phytagar (6, 7, 8, and 9 g L-1) and gellan gum (2, 3, 4, and 5 g L-1) were examined for efficient shoot formation. The results revealed the superiority of half-strength MS basal medium in shoot organogenesis and growth of Liriope platyphylla. But the half-strength B5 media performed poorly. Compared to plant agar, gellan gum performed well in terms of shoot regeneration and shoot length. When gellan gum was used at 3 g L-1 the maximum number of shoots explant-1 (5.8) and longest shoot (45.8 mm) was observed but the lowest number of shoots explant-1 (3.2) and shortest shoot (21.4 mm) was registered with 5 g L-1. It is proposed from our study that half-strength MS media and gellan gun gelling agent at 3 g/l could be applied in shoot organogenesis and growth of L. platyphylla.
鹅掌楸可以通过播种或分割其块茎根来繁殖。本研究采用培养基和胶凝剂，建立了以分生组织外植体为外植体的桔梗植株芽器官发生方法。为了选择芽器官发生的最佳培养基条件，考察了全强度和半强度B5、SH和MS培养基的影响。研究了不同浓度的胶凝剂，如植酸酶（6、7、8和9 g L-1）和结冷胶（2、3、4和5 g L-1）的有效芽形成。结果表明，半强度MS基础培养基在鹅掌楸枝条器官发生和生长方面具有优越性。但强度为一半的B5介质表现不佳。与植物琼脂相比，结兰胶在芽再生和芽长方面表现良好。当胶兰胶以3g L-1使用时，观察到芽外植体-1的最大数量（5.8）和最长芽（45.8mm），但以5g L-1记录的芽外植体1的最低数量（3.2）和最短芽（21.4mm）。我们的研究表明，半强度MS培养基和3g/l的结冷枪胶凝剂可以用于白桦的枝条器官发生和生长。
{"title":"Effect of media and gelling agents on shoot organogenesis of Liriope platyphylla","authors":"Young Chang Kim, W. Park, R. Sathasivam, H. Kim, J. K. Kim, Sang-Un Park","doi":"10.25081/jp.2023.v15.8319","DOIUrl":"https://doi.org/10.25081/jp.2023.v15.8319","url":null,"abstract":"Liriope platyphylla can be multiplied either by planting seeds or dividing its tuberous roots. In this study, a method for L. platyphylla plant shoot organogenesis from meristem explants was developed employing medium and gelling agents. For the selection of optimal medium conditions for shoot organogenesis, the effects of full- and half-strength B5, SH, and MS media were examined. Different concentrations of the gelling agents such as phytagar (6, 7, 8, and 9 g L-1) and gellan gum (2, 3, 4, and 5 g L-1) were examined for efficient shoot formation. The results revealed the superiority of half-strength MS basal medium in shoot organogenesis and growth of Liriope platyphylla. But the half-strength B5 media performed poorly. Compared to plant agar, gellan gum performed well in terms of shoot regeneration and shoot length. When gellan gum was used at 3 g L-1 the maximum number of shoots explant-1 (5.8) and longest shoot (45.8 mm) was observed but the lowest number of shoots explant-1 (3.2) and shortest shoot (21.4 mm) was registered with 5 g L-1. It is proposed from our study that half-strength MS media and gellan gun gelling agent at 3 g/l could be applied in shoot organogenesis and growth of L. platyphylla.","PeriodicalId":16777,"journal":{"name":"Journal of Phytology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48920721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-20DOI: 10.25081/jp.2023.v15.7902
R. M. Emon, M. N. Sakib, M. Khatun, M. A. Malek, Md. S. Haque, Md. Amirul Alam
Identification of genetic diversity and their relationships among breeding materials is crucial in crop improvement strategies. In this study, 38 sesame genotypes were characterized for their genetic diversity. The results revealed significant variations among various traits such as plant height, maturity, capsule plant-1 and seeds capsule-1. The number of capsule plant-1 showed significant positive correlation with seeds capsule-1. The highest heritability was found for the numbers of capsules plant-1 (98.67%). The 38 genotypes were separated into six distinct clusters. Comparison within the populations of the cluster IV and those of cluster VI had the highest capsules plant-1, seeds capsule-1 with enormous genetic diversity. For molecular characterization, 7 microsatellite markers and 5 SSR primers with polymorphism were finally chosen for genetic diversity analysis. Altogether, 19 alleles were identified among the 38 genotypes, and the average number of alleles per locus was 3.80. The lowest and the highest numbers of alleles were 3 and 5, respectively. The polymorphism information content (PIC) ranged from 0.3201 to 0.5934 and SI-ssr30 showed to be highest at 0.5934. The UPGMA based clustering depicted a significant variation at molecular level among the sesame genotypes, having a coefficient of similarity between 0.29 and 1.00. The present study confirmed that extensive genetic diversity existed among the sesame genotypes and valuable agronomic traits may result in the development of high yielding genotypes.
{"title":"Microsatellite marker assisted molecular and morpho-physiological genetic diversity assessment in 38 genotypes of sesame (Sesamum indicum L.)","authors":"R. M. Emon, M. N. Sakib, M. Khatun, M. A. Malek, Md. S. Haque, Md. Amirul Alam","doi":"10.25081/jp.2023.v15.7902","DOIUrl":"https://doi.org/10.25081/jp.2023.v15.7902","url":null,"abstract":"Identification of genetic diversity and their relationships among breeding materials is crucial in crop improvement strategies. In this study, 38 sesame genotypes were characterized for their genetic diversity. The results revealed significant variations among various traits such as plant height, maturity, capsule plant-1 and seeds capsule-1. The number of capsule plant-1 showed significant positive correlation with seeds capsule-1. The highest heritability was found for the numbers of capsules plant-1 (98.67%). The 38 genotypes were separated into six distinct clusters. Comparison within the populations of the cluster IV and those of cluster VI had the highest capsules plant-1, seeds capsule-1 with enormous genetic diversity. For molecular characterization, 7 microsatellite markers and 5 SSR primers with polymorphism were finally chosen for genetic diversity analysis. Altogether, 19 alleles were identified among the 38 genotypes, and the average number of alleles per locus was 3.80. The lowest and the highest numbers of alleles were 3 and 5, respectively. The polymorphism information content (PIC) ranged from 0.3201 to 0.5934 and SI-ssr30 showed to be highest at 0.5934. The UPGMA based clustering depicted a significant variation at molecular level among the sesame genotypes, having a coefficient of similarity between 0.29 and 1.00. The present study confirmed that extensive genetic diversity existed among the sesame genotypes and valuable agronomic traits may result in the development of high yielding genotypes.","PeriodicalId":16777,"journal":{"name":"Journal of Phytology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48633907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-14DOI: 10.25081/jp.2023.v15.8196
C. Park, H. Yeo, Min-Soon Choi, R. Sathasivam, Md. Romij Uddin, M. Muthuraman, H. Kim, Sang-Un Park
Buckwheat (Fagopyrum esculentum Monech) contains several secondary metabolites like phenolic chemicals. Pectin has been demonstrated to be an efficient elicitor from the biotic group for triggering the defensive response, which enhances the production of secondary metabolites. In this study, the effect of pectin on the growth of buckwheat sprouts and the production of phenylpropanoid compounds in common buckwheat sprouts was investigated by using high-performance liquid chromatography (HPLC). Pectin treatments of 0, 2, 4, 6, and 8 mg/L were administered on buckwheat sprouts for ten days to assess the growth characteristics and optimum concentrations. In comparison to the control treatment, 2 mg/L pectin enhances the shoot length by 24%. But when pectin concentration continued to rise, a tendency toward shorter shoots was seen. Pectin treatment decreased the fresh weight of the sprout as compared to the control treatment. The phenylpropanoid accumulation in buckwheat sprouts varied depending on the amount of pectin utilized. Pectin treatment at 6 mg/L resulted in a 15.10% increase in total phenylpropanoid accumulation. The findings of this study indicate that pectin is a possible elicitor, however, more research on how pectin affects the buildup of phenylpropanoids in buckwheat sprouts would be more intriguing to examine the implications of this work.
{"title":"Influence of pectin on phenylpropanoid accumulation in buckwheat (Fagopyrum esculentum) sprout","authors":"C. Park, H. Yeo, Min-Soon Choi, R. Sathasivam, Md. Romij Uddin, M. Muthuraman, H. Kim, Sang-Un Park","doi":"10.25081/jp.2023.v15.8196","DOIUrl":"https://doi.org/10.25081/jp.2023.v15.8196","url":null,"abstract":"Buckwheat (Fagopyrum esculentum Monech) contains several secondary metabolites like phenolic chemicals. Pectin has been demonstrated to be an efficient elicitor from the biotic group for triggering the defensive response, which enhances the production of secondary metabolites. In this study, the effect of pectin on the growth of buckwheat sprouts and the production of phenylpropanoid compounds in common buckwheat sprouts was investigated by using high-performance liquid chromatography (HPLC). Pectin treatments of 0, 2, 4, 6, and 8 mg/L were administered on buckwheat sprouts for ten days to assess the growth characteristics and optimum concentrations. In comparison to the control treatment, 2 mg/L pectin enhances the shoot length by 24%. But when pectin concentration continued to rise, a tendency toward shorter shoots was seen. Pectin treatment decreased the fresh weight of the sprout as compared to the control treatment. The phenylpropanoid accumulation in buckwheat sprouts varied depending on the amount of pectin utilized. Pectin treatment at 6 mg/L resulted in a 15.10% increase in total phenylpropanoid accumulation. The findings of this study indicate that pectin is a possible elicitor, however, more research on how pectin affects the buildup of phenylpropanoids in buckwheat sprouts would be more intriguing to examine the implications of this work.","PeriodicalId":16777,"journal":{"name":"Journal of Phytology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48399654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-07DOI: 10.25081/jp.2023.v15.8020
A. Singh, Sheenu Sharma, Sabir Hussain, D. Rai
The Elaeocarpus is considered the largest genus in the family Elaeocarpaceae. It is commonly called ‘Rudraksha’ in India and has a noteworthy attachment with the Indians, mostly Hindus. The plant is known for its beneficial aspects and supply of various ecosystem services, particularly in medicinal sciences. The present study provides a comprehensive review of the genus Elaeocarpus for its ecosystem services in the context of human wellbeing. A literature survey was conducted from 1970 to 2020 on internet scientific databases such as Scopus, Science Direct, Google Scholar, PubMed, and Web of Science using strings such as, Elaeocarpus, Rudraksha, medicine, conservation, tradition, and services. One hundred two publications were considered for data extraction and finalising the review. Firstly, we classified the ecosystem services under respective classes as provided by MEA (2005), and the remaining benefits that didn’t fit under the classification were presented separately. It has been reported that Rudraksha delivers all categories of ecosystem services: provisioning, regulating, cultural, and supporting services. In addition, the Rudraksha plant owes a high status in medicinal science, ayurveda, and religious mythology. In the last five decades, studies were conducted on various species of Elaeocarpus for their beneficial aspects. It was found that Elaeocarpus plants have a vital role in ayurveda, pharmaceuticals, and pharmacological and astrological science. In addition, they have been used as food, firewood, timber, and the production of secondary metabolites and their role in cultural and religious dimensions are very clearly discussed. The provisioning and cultural services delivered by Rudraksha constitute only 30% and 10% of the services mentioned in MEA (2005). However, only a little research has been conducted on regulatory and supporting services provided by Rudraksha. Therefore, for a long-term sustainability and multifunctionality assessment, the extension of ecosystem services regarding individual plant’s (tree) services should be highly recommended. Hence, the present review investigates the scientific knowledge about the ecosystem services of the Elaeocarpus.
Elaeocarpus被认为是elaeocarpacae科中最大的属。在印度，它通常被称为“Rudraksha”，与印度人(主要是印度教徒)有着值得注意的联系。这种植物以其有益的方面和提供各种生态系统服务而闻名，特别是在医学科学方面。本研究提供了一个全面的审查属Elaeocarpus在人类福祉的背景下，其生态系统服务。从1970年到2020年，对Scopus、Science Direct、b谷歌Scholar、PubMed和Web of Science等互联网科学数据库进行了文献调查，使用了Elaeocarpus、Rudraksha、medicine、conservation、tradition和services等字符串。考虑了102份出版物进行数据提取和最后审查。首先，根据MEA(2005)的分类，将生态系统服务功能划分为不同的类别，其余不适合该分类的生态系统服务功能分别进行了分类。据报道，Rudraksha提供所有类型的生态系统服务:供应、调节、文化和支持服务。此外，Rudraksha植物在医学科学、阿育吠陀和宗教神话中占有很高的地位。在过去的五十年里，人们对不同种类的树果进行了研究，以了解其有益的方面。人们发现，菖蒲属植物在阿育吠陀、制药、药理学和占星术科学中具有重要作用。此外，它们还被用作食物、柴火、木材和次生代谢物的生产，它们在文化和宗教方面的作用也得到了非常清楚的讨论。Rudraksha提供的供应和文化服务仅占MEA(2005)中提到的服务的30%和10%。然而，对Rudraksha提供的监管和支持服务进行的研究很少。因此，为了长期的可持续性和多功能性评估，应强烈建议将生态系统服务扩展到单个植物(树)服务。因此，本文对榆木属植物生态系统服务功能的科学认识进行了综述。
{"title":"A comprehensive analysis on the ecosystem services of Elaeocarpus L. (Elaeocarpaceae): a review","authors":"A. Singh, Sheenu Sharma, Sabir Hussain, D. Rai","doi":"10.25081/jp.2023.v15.8020","DOIUrl":"https://doi.org/10.25081/jp.2023.v15.8020","url":null,"abstract":"The Elaeocarpus is considered the largest genus in the family Elaeocarpaceae. It is commonly called ‘Rudraksha’ in India and has a noteworthy attachment with the Indians, mostly Hindus. The plant is known for its beneficial aspects and supply of various ecosystem services, particularly in medicinal sciences. The present study provides a comprehensive review of the genus Elaeocarpus for its ecosystem services in the context of human wellbeing. A literature survey was conducted from 1970 to 2020 on internet scientific databases such as Scopus, Science Direct, Google Scholar, PubMed, and Web of Science using strings such as, Elaeocarpus, Rudraksha, medicine, conservation, tradition, and services. One hundred two publications were considered for data extraction and finalising the review. Firstly, we classified the ecosystem services under respective classes as provided by MEA (2005), and the remaining benefits that didn’t fit under the classification were presented separately. It has been reported that Rudraksha delivers all categories of ecosystem services: provisioning, regulating, cultural, and supporting services. In addition, the Rudraksha plant owes a high status in medicinal science, ayurveda, and religious mythology. In the last five decades, studies were conducted on various species of Elaeocarpus for their beneficial aspects. It was found that Elaeocarpus plants have a vital role in ayurveda, pharmaceuticals, and pharmacological and astrological science. In addition, they have been used as food, firewood, timber, and the production of secondary metabolites and their role in cultural and religious dimensions are very clearly discussed. The provisioning and cultural services delivered by Rudraksha constitute only 30% and 10% of the services mentioned in MEA (2005). However, only a little research has been conducted on regulatory and supporting services provided by Rudraksha. Therefore, for a long-term sustainability and multifunctionality assessment, the extension of ecosystem services regarding individual plant’s (tree) services should be highly recommended. Hence, the present review investigates the scientific knowledge about the ecosystem services of the Elaeocarpus.","PeriodicalId":16777,"journal":{"name":"Journal of Phytology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47054298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-02-24DOI: 10.25081/jp.2023.v15.7641
Rahmad Fadli, S. Suwandi, Nurhayati Nurhayati, A. Muslim, C. Irsan
Basal stem rot caused by a soil-borne fungus, Ganoderma boninense, is one of the most severe diseases in monoculture oil palm plantations. The effect of mixed cropping with water yam on infection and inoculum potential of Ganoderma boninense on oil palm seedlings was assessed under a pot experiment. Plant inoculation with rubber woodblock (RWB)-colonized Ganoderma induced disease on both mixed cropped hosts (multiple host infection), but with less severe root necrotic and less plant mortality relative to inoculation on a single oil palm host. Inoculum potential of the pathogen was reduced under mixed cropping, as shown by more considerable RWB decay than a single host, and the fungal survival was suppressed to be 55% for 12 months trial. Water yam mixed cropping did not interfere with the growth of the inoculated oil palm seedling. This study revealed the multiple host infection under mixed cropping with water yam suppressed damage caused by Ganoderma boninense to oil palm as the primary host.
{"title":"Effect of mixed cropping of water yam (Dioscorea alata) on Ganoderma disease of oil palm","authors":"Rahmad Fadli, S. Suwandi, Nurhayati Nurhayati, A. Muslim, C. Irsan","doi":"10.25081/jp.2023.v15.7641","DOIUrl":"https://doi.org/10.25081/jp.2023.v15.7641","url":null,"abstract":"Basal stem rot caused by a soil-borne fungus, Ganoderma boninense, is one of the most severe diseases in monoculture oil palm plantations. The effect of mixed cropping with water yam on infection and inoculum potential of Ganoderma boninense on oil palm seedlings was assessed under a pot experiment. Plant inoculation with rubber woodblock (RWB)-colonized Ganoderma induced disease on both mixed cropped hosts (multiple host infection), but with less severe root necrotic and less plant mortality relative to inoculation on a single oil palm host. Inoculum potential of the pathogen was reduced under mixed cropping, as shown by more considerable RWB decay than a single host, and the fungal survival was suppressed to be 55% for 12 months trial. Water yam mixed cropping did not interfere with the growth of the inoculated oil palm seedling. This study revealed the multiple host infection under mixed cropping with water yam suppressed damage caused by Ganoderma boninense to oil palm as the primary host.","PeriodicalId":16777,"journal":{"name":"Journal of Phytology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49112993","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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