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Genetic identification of an invasive plant ( Parthenium hysterophorus) and its crude extract phytotoxicity against palm oil weed ( Diodia ocimifolia) 入侵植物Parthenium hysterophorus的遗传鉴定及其对棕榈油杂草(didia ocimifolia)的毒力
IF 1.1 Q3 AGRONOMY Pub Date : 2023-03-22 DOI: 10.24425/jppr.2022.141361
This paper reports a study on genetic identification of Parthenium hysterophorus and the ability of P. hysterophorus crude extract (PHCE) to inhibit germination of weeds in palm oil plantations, particularly slender buttonweed ( Diodia ocimifolia ). Parthenium hysterophorus is a noxious plant with invasive properties. It has become one of the world’s seven most devastating and hazardous weed‑s due to its allelopathic properties. However, a compre‑ hensive study on its genetic identification and its herbicidal activity against weeds in palm oil plantations had never been carried out. In the present study, internal transcribes spacer (ITS) region‑based analysis was used as a molec ular marker to ascertain the local Rumput Miang Mexico (RMM) as a P. hysterophorus species. From the results, the DNA sequence obtained was 99% matched with P. hysterophorus species database. In the dose‑response bioassay study, the phytotoxicity of PHCE on D. ocimifolia was simulated under laboratory conditions. Methanolic extract of P. hysterophorus significantly inhibited germination and growth of shoots and radicles of D. ocimifolia at low concentrations (1−5 g ∙ l −1 ). A study on the herbicidal activity of PHCE could be an additional management approach for this phy‑ totoxic species and an alternative way to naturally control weeds on palm oil plantations.
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引用次数: 0
Water retention on the surface of apples and sweet cherry leaves and fruits 苹果和甜樱桃的叶子和水果表面的水分潴留
IF 1.1 Q3 AGRONOMY Pub Date : 2023-03-22 DOI: 10.24425/jppr.2022.141352
Surface water retention of leaves and fruits of apple ( Malus domestica Borkh.) and sweet cherry ( Prunus avium L.), was evaluated under controlled environmental conditions in order to determine the retention potential at different growth stages. Dipping and spraying, with and without non-ionic surfactant, were used as application systems. Water retention was expressed as the ratio between the weight difference of the organ before and post application and organ weight before application. Leaf water retention by dipping was 62 and 64% for ‘Royal Gala’ and ‘Fuji’ apples, respectively, and 37 and 50% by spraying. The surfactant tended to reduce foliar water retention by spraying on both species. An exponential reduction of fruit water retention was observed during their growth. Fruit dipping generated the highest water retention, with values of 50% at the earliest stage. Then, water retention stabilized at 1–2%, when the apples and sweet cherries diameter reached 25 and 15 mm, respectively, despite dipping or spraying. The surfactant tended to increase water retention at early fruit stages and to reduce it with fruit growth. These results can be useful for estimating the potential residue on leaves and fruits in apple and sweet cherry trees, in both the orchard (spraying) and the packing house (dipping).
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引用次数: 0
Evaluation of the antagonistic potential of Trichoderma spp. against Fusarium oxysporum F.28.1A 木霉对尖孢镰刀菌拮抗潜力的评价
IF 1.1 Q3 AGRONOMY Pub Date : 2023-03-22 DOI: 10.24425/jppr.2023.144502
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引用次数: 0
Effects of different management practices of organic uphill grasslands on the abundance and diversity of soil mesofauna 有机上坡草地不同管理方式对土壤中动物群丰度和多样性的影响
IF 1.1 Q3 AGRONOMY Pub Date : 2023-03-22 DOI: 10.24425/JPPR.2018.124652
I. Gruss, Karolina Pastuszko, J. Twardowski, M. Hurej
In this study the effect of different grassland managements (cattle grazing with different intensities and mowing) on soil mesofauna, i.e. mites (Acari) and springtails (Collembola), was studied. Mites and springtails are the most numerous representatives of soil mesofauna organisms living in the upper soil layers (up to 5 cm). Soil mesofauna groups or species are commonly used as bioindicators of soil health. The experiment was carried out from 2007 to 2009 in the West Sudety Mountains, Poland. Pastures and meadows were under organic farming management, without pesticides or synthetic fertilizers, and restricted livestock density. Soil samples were taken three times a year (in May−June, July and October) from pastures grazed at different frequencies: once, twice and four times a year, alternate management (grazed and mown pasture) and mown meadow. Mites were identified according to orders or suborders (Oribatida, Gamasida, Prostigmata, Astigmata), while springtails to the species level. The data were analysed using a general linear model (GLM). The mesofauna taxa in relation to the treatment and date were analysed with the canonical correspondence analysis (CCA). The data from three years showed that most soil mesofauna assemblages occurred in significantly higher numbers on the pasture grazed once or twice and on alternate managed pasture than in pasture grazed four times a year and mown meadow. The CCA analysis showed the preference of most springtail species to pasture grazed once a year, while mites preferred pasture grazed twice a year and alternate management. The number of species and the abundance of the most numerous species (Protaphorura pannonica, Desoria multisetis and Folsomides parvulus) did not differ significantly between treatments. To summarize, cattle grazing once or twice a season or alternate management (grazing and mowing once a season) have a positive impact on soil mesofauna.
本文研究了不同草地管理(不同强度放牧和刈割)对土壤中系动物螨(蜱螨)和弹尾虫(弹尾虫)的影响。螨虫和弹尾虫是生活在土壤上层(5厘米以下)的土壤中系生物中数量最多的代表。土壤中系动物群或物种通常被用作土壤健康的生物指标。该实验于2007年至2009年在波兰西苏台德山脉进行。牧场和草地采用有机农业管理,不使用农药和合成肥料,并限制牲畜密度。每年3次(5 - 6月、7月和10月)从放牧频率不同的牧场采集土壤样本:每年1次、2次和4次,交替管理(放牧和刈割牧场)和刈割草甸。螨类按甲螨目、革螨目、原螨目、无毛螨目划分,弹尾目按种划分。使用一般线性模型(GLM)对数据进行分析。用典型对应分析(CCA)分析了与处理和日期有关的中系动物类群。3年数据表明,一年放牧1次或2次的草地和轮换放牧的草地土壤中动物群的数量明显高于一年放牧4次的草地和刈割的草地。CCA分析结果表明,大多数春尾虫偏好一年放牧一次,而螨虫则偏好一年放牧两次和交替管理。不同处理间的物种数量和最丰富的物种丰度无显著差异(Protaphorura pannonica, Desoria multisetis和Folsomides parvulus)。总而言之,每季放牧一次或两次或交替管理(每季放牧和割草一次)对土壤中系动物有积极影响。
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引用次数: 2
Molecular characterization of Cucumber mosaic virus sub group IB infecting Cavendish banana plants in Ethiopia 侵染埃塞俄比亚卡文迪什香蕉的黄瓜花叶病毒IB亚群的分子特征
IF 1.1 Q3 AGRONOMY Pub Date : 2023-03-22 DOI: 10.24425/jppr.2021.139249
Yohanis Kebede, S. Majumder
Banana is the major fruit crop produced in Ethiopia. Since Cucumber mosaic virus (CMV) is one of the most devastating plant viruses infecting banana, the present study was undertaken to survey and identify CMV strains infecting banana plants in Ethiopia. Dot immune-binding assay (DIBA) and reverse transcription-polymerase chain reaction (RT-PCR) revealed the presence of CMV in all of the symptomatic samples tested. The results of sequence and phylogenetic analysis revealed that the isolate under study was a CMV isolate from the IB subgroup. Multiple sequence alignment revealed a three nucleotide sequence variation that could be used to distinguish CMV subgroups. Selection pressure analysis showed the CMV-RNA1 region undergoing positive selection pressure. Tajima`s test of neutrality revealed a positive value of 0.86468 indicating CMV population contraction. To the best of our knowledge, this is the first report and molecular characterization of CMV IB subgroup isolate infecting banana plants in Ethiopia.
香蕉是埃塞俄比亚生产的主要水果作物。由于黄瓜花叶病毒(CMV)是感染香蕉的最具破坏性的植物病毒之一,本研究旨在调查和鉴定感染埃塞俄比亚香蕉植物的CMV菌株。点免疫结合试验(DIBA)和逆转录聚合酶链式反应(RT-PCR)显示,在所有测试的症状样本中都存在CMV。序列和系统发育分析结果表明,所研究的分离物是来自IB亚群的CMV分离物。多序列比对显示了一个可用于区分CMV亚群的三核苷酸序列变异。选择压力分析显示CMV-RNA1区域经历正选择压力。Tajima的中性测试显示阳性值为0.86468,表明CMV种群收缩。据我们所知,这是埃塞俄比亚首次报道感染香蕉植物的CMV IB亚群分离物及其分子特征。
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引用次数: 0
Characterization by GC/MS-FID and GC/MS-HS-SPME and insecticidal activity against Callosobruchus maculatus (Fabricius, 1775) of essential oils and powder of Xylopia aethiopica (Dunal) A. Rich from Senegal 用GC/MS-FID和GC/MS-HS-SPME表征塞内加尔木霉精油和粉末的杀虫活性
IF 1.1 Q3 AGRONOMY Pub Date : 2023-03-22 DOI: 10.24425/jppr.2021.137955
A. Thiam, M. Guèye, Cheikhna Hamala Sangharé, Papa Seyni Cissokho, Elhadji Barka Ndiaye, Serigne Mbacké Diop, M. Diop, I. Ndiaye, M. Fauconnier
Today the use of plant extracts, in particular essential oils, is a natural alternative to synthetic insecticides in the fight against crop pests. In this study, the insecticidal activity of essential oils and powder of Xylopia aethiopica (Annonaceae) were tested by both fumigation and contact against Callosobruchus maculatus. The essential oil of X. aethiopica, obtained by steam distillation and the powder, with a particle size of 1 mm, were used for the tests. The analysis of essential oils and powder of X. aethiopica by GC-MS/FID and GC/MS-HS-SPME, showed that the main compounds were β-pinene (28.9–19.0%), 1,8-ci neole (14.9–7.6%) and α-pinene (9.8–19.4%). Insecticidal activity of essential oils and powder of X. aethiopica, respectively, by fumigation (F) and contact (C) against C. maculatus showed toxicity LD50 = 0.2 ± 0.0 μl · cm –3, LT50 = 16.4 ± 1.2 h (F) and LD50 = 9.2 ± 0.7 g · kg –1, LT50 = 69.6 ± 0.4 h (C). The essential oil and powder of X. aethiopica can be considered as bio-insecticides against C. maculatus for the protection of cowpeas in Senegal.
如今,使用植物提取物,特别是精油,是对抗作物害虫的合成杀虫剂的天然替代品。本研究采用熏蒸和接触两种方法,测定了番荔枝科植物香精油和粉体对斑蝥的杀虫活性。使用通过蒸汽蒸馏获得的X.aethiopica精油和粒度为1mm的粉末进行测试。采用GC-MS/FID和GC/MS-HS-SPME对异尖线虫的精油和粉末进行分析,结果表明,其主要成分为β-蒎烯(28.9–19.0%)、1,8-新烯(14.9–7.6%)和α-蒎烷(9.8–19.4%),LT50=16.4±1.2 h(F),LD50=9.2±0.7 g·kg–1,LT50=69.6±0.4 h(C)。在塞内加尔,A.ethiopica精油和粉末可以被认为是防治斑蝥的生物杀虫剂,用于保护豇豆。
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引用次数: 0
Stress-tolerant antagonistic plant growth-promoting rhizobacteria from Zea mays 玉米耐胁迫拮抗植物促生长根瘤菌
IF 1.1 Q3 AGRONOMY Pub Date : 2023-03-22 DOI: 10.24425/119127
A. Kaur, S. Devi, P. Vyas
Biological control of plant diseases is strongly emerging as an effective alternative to the use of chemical pesticides and fungicides. Stress tolerance is an important attribute in the selection of bacteria for the development of microbial inoculants. Fourteen salt-tolerant bacteria showing different morphological features isolated from the rhizosphere of maize were evaluated for different plant growth-promoting activities. All isolates showed auxin production ranging from 5 to 24 μg ⋅ ml–1 after 48 h incubation in tryptophan supplemented media. Phosphate solubilization ranged from 15 to 419 μg ⋅ ml–1. 1-aminocycloproprane1-carboxylate (ACC) deaminase activity was shown by 6 isolates, ammonia production by 9 isolates, siderophore production by 8 isolates while HCN production by 4 isolates. Four bacterial isolates with all plant growth-promoting properties also showed strong antagonistic activities against Fusarium oxysporum, F. verticillioides, Curvularia lunata and Alternaria alternata and abiotic stress tolerance against salinity, temperature, pH and calcium salts. Two selected bacterial isolates significantly enhanced the growth of pea and maize test plants under greenhouse conditions. The bacterial isolate M1B2, which showed the highest growth promotion of test plants, was identified as Bacillus sp. based on phenotypic and 16S rDNA gene sequencing. The results indicated that Bacillus sp. M1B2 is a potential candidate for the development of microbial inoculants in stressful environments.
生物防治植物病害正迅速成为化学农药和杀菌剂的有效替代品。抗逆性是微生物接种剂开发中选择细菌的一个重要指标。从玉米根际分离出14株形态特征不同的耐盐菌,对其促生长活性进行了评价。在色氨酸补充培养基中培养48 h后,所有菌株的生长素产量均在5 ~ 24 μg⋅ml-1之间。磷酸盐增溶量为15 ~ 419 μg⋅ml-1。6株菌株具有1-氨基环丙酸-羧酸脱氨酶活性,9株菌株产氨,8株菌株产铁载体,4株菌株产HCN。4株具有促进植物生长特性的分离菌对尖孢镰刀菌、黄萎病镰刀菌、月曲孢菌和互交菌也表现出较强的拮抗活性,并对盐、温度、pH和钙盐具有非生物抗性。两株菌株在温室条件下显著促进了豌豆和玉米试验植株的生长。经表型分析和16S rDNA基因测序,鉴定菌株M1B2为芽孢杆菌。结果表明,芽孢杆菌M1B2是开发胁迫环境下微生物接种剂的潜在候选菌株。
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引用次数: 11
Endophytic colonization by Beauveria bassiana and Metarhizium anisopliae induces growth promotion effect and increases the resistance of cucumber plants against Aphis gossypii 球孢白僵菌和金龟子绿僵菌的内生定殖可促进黄瓜生长,增强黄瓜对棉蚜的抗性
IF 1.1 Q3 AGRONOMY Pub Date : 2023-03-22 DOI: 10.24425/jppr.2021.139244
The entomopathogenic fungi (EPF) are characterized as fungi with various functions and numerous mechanisms of action. The ability to establish themselves as beneficial endo-phytes provides a sound ground for their exploitation in crop production and protection. The purpose of this study was to evaluate the entomopathogenic strains of Beauveria bas­ siana and Mertarhizium anisopliae for their potential to colonize cucumber plants under natural environmental conditions in non-sterile substrate. Seed submersion in conidial suspension resulted in systemic colonization of cucumber plants 28 days post-inoculation. Scanning electron microscope micrographs demonstrated that conidia of both fungal genera have adhered, germinated and directly penetrated seed epidermal cells 24 hr post-sub-mersion. Treated with EPF cucumber seeds resulted seedlings tissues of which contained a significantly higher amount of total phenolic compounds and unchanged amounts of chlorophylls. There was a significant negative effect of endophytic colonization on the Aphis gossypii population size after 5 days of exposure as well as a positive effect on cucumber growth and development 7 weeks post-inoculation. We suggest that reduction of A. gossypii population on mature Cucumis sativus plants is caused via an endophyte-triggered improvement of plant’s physiological parameters such as enhanced plant growth with subsequent increase in plant resistance through augmented production of phenolic compounds.
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引用次数: 8
A comparative study of native growth-promoting rhizobacteria and commercial biofertilizer on maize ( Zea mays L.) and wheat ( Triticum aestivum L.) development in a saline environment 原生促生根瘤菌与商业生物肥料在盐碱化环境下对玉米和小麦发育影响的比较研究
IF 1.1 Q3 AGRONOMY Pub Date : 2023-03-22 DOI: 10.24425/jppr.2022.140296
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引用次数: 1
Temporal variation and spatial distribution of Piezodorus guildinii (Weestwood, 1837) in soybean ( Glycine max (L.) Merrill) 大豆(Glycine max (L.))中Piezodorus guildinii (westwood, 1837)的时空变化与空间分布美林)
IF 1.1 Q3 AGRONOMY Pub Date : 2023-03-22 DOI: 10.24425/jppr.2022.141357
The red-banded stink bug, Piezodorus guildinii , is an important pest in soybean. Information on its distribution is needed to determine the most effective timing for pest control and strategies to avoid yield losses, such as adequate monitoring. The present study was aimed at examining the temporal variation and spatial distribution of P. guildinii in soybean. The experimental design comprised an area of 5400 m² planted with soybean, with 54 plots of 100 m² each, in which plants were examined weekly for nymphs and adults of P. guildinii with a beating sheet. Evaluations were carried out from soybean emergence to harvest; however, P. guildinii occurred only during the reproductive stage. Based on aggregation indices, theoretical frequency distributions, and semivariograms, nymphs and adults were randomly distributed at the beginning of infestation but, tended to be aggregate during pod setting and seed filling. Our findings have a contribution to improving pest sampling systems and infestation mapping, including future semiochemi-cals studies.
{"title":"Temporal variation and spatial distribution of Piezodorus guildinii (Weestwood, 1837) in soybean ( Glycine max (L.) Merrill)","authors":"","doi":"10.24425/jppr.2022.141357","DOIUrl":"https://doi.org/10.24425/jppr.2022.141357","url":null,"abstract":"The red-banded stink bug, Piezodorus guildinii , is an important pest in soybean. Information on its distribution is needed to determine the most effective timing for pest control and strategies to avoid yield losses, such as adequate monitoring. The present study was aimed at examining the temporal variation and spatial distribution of P. guildinii in soybean. The experimental design comprised an area of 5400 m² planted with soybean, with 54 plots of 100 m² each, in which plants were examined weekly for nymphs and adults of P. guildinii with a beating sheet. Evaluations were carried out from soybean emergence to harvest; however, P. guildinii occurred only during the reproductive stage. Based on aggregation indices, theoretical frequency distributions, and semivariograms, nymphs and adults were randomly distributed at the beginning of infestation but, tended to be aggregate during pod setting and seed filling. Our findings have a contribution to improving pest sampling systems and infestation mapping, including future semiochemi-cals studies.","PeriodicalId":16848,"journal":{"name":"Journal of Plant Protection Research","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2023-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42727908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
Journal of Plant Protection Research
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