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BIOHM Probiotics Retain Viability in Low pH Environments Simulating the Digestive Environment BIOHM益生菌在模拟消化环境的低pH环境中保持活力
Pub Date : 2019-01-01 DOI: 10.35248/2329-8901.19.7.211
M. Ghannoum, A. Ghannoum, L. Long, Sun Pl, N. Isham
Probiotics in various combinations and in various forms have become popular supplements for establishing and maintaining gut health. However, commercial products vary in the effectiveness of their specific probiotic strains and the ability of these strains to survive the acidic environment of the stomach. A novel probiotic, manufactured by BIOHM, LLC, has a unique formulation of Saccharomyces boulardii, Lactobacillus acidophilus, L. rhamnosus, and Bifidobacterium breve, in combination with amylase, which has been developed to re-balance the bacterial and fungal population of the human gastrointestinal tract and combat digestive biofilms formed by pathogenic bacteria and fungi. Our data shows that these strains have the ability to survive the acidic environment when taken within 30 minutes of meal, a factor that is vital to ensure probiotic effectiveness.
各种组合和形式的益生菌已成为建立和维持肠道健康的流行补充剂。然而,商业产品在其特定益生菌菌株的有效性和这些菌株在胃的酸性环境中生存的能力方面各不相同。BIOHM, LLC生产的一种新型益生菌,具有博拉氏酵母菌、嗜酸乳杆菌、鼠李糖乳杆菌和短双歧杆菌的独特配方,与淀粉酶结合,用于重新平衡人类胃肠道的细菌和真菌种群,并对抗由致病菌和真菌形成的消化生物膜。我们的数据显示,这些菌株在饭后30分钟内就能在酸性环境中存活下来,这是确保益生菌有效性的关键因素。
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引用次数: 1
Potential Using of Ulvan Polysaccharide from Ulva lactuca as a Prebiotic in Synbiotic Yogurt Production 乳Ulva Ulvan多糖作为益生元在合成酸奶生产中的潜在应用
Pub Date : 2019-01-01 DOI: 10.35248/2329-8901.19.7.208
M. SamahShalaby, H. HadearAmin
Background and objective: There is growing interesting for edible marine algae species as a source for beneficial polysaccharides. Ulva lactuca is a widespread macroalga and highly consumed by humans and livestock because of its great nutritional values. Many studies showed that different extracts of U. lactuca possess nutritional and biological values. Ulvan is one of its structural water-soluble sulfated polysaccharides. The main objective of this study is preparing synbiotic yogurt by using ulvan polysaccharide by different percentage as a prebiotic ingredient and using probiotic starter bacteria in the manufacture of set synbiotic yogurt. Then study the quality properties of the resultant synbiotic yogurt. Methodology: ulvan polysaccharide was extracted from Ulva lactuca using the methods of hot water-extraction and ethanol-precipitation. The synbiotic yogurt was prepared from fresh skim milk and ulvan polysaccharide as prebiotics, which was added by different concentrations: 1%, 2% and 4% (w/v). The mixtures were heated to 90°C for 10 min and immediately cooled to 39°-40°C; and inoculated with 3% of probiotic starter culture (containing of Lactobacillus acidophilus LA-5 Streptococcus thermophilus TH-4, and Bifidobacterium sp. Bb-12). The inoculated milks were distributed into 100 mL plastic cups and incubated at 40°C till complete coagulation. The synbiotic yogurt were transferred to the refrigerator at 7° ± 1°C and storage for 9 days; then the treatments were analyzed for the bacteriological, physical, chemical, and organoleptic assessments at fresh time and after 9 days of cold storage. Results: The results showed that adding of 1-2% of the ulvan polysaccharide presented synbiotic yogurt with good chemical and physical properties, as well as stimulated the growth and activity of probiotic bacteria. But the addition of high percentage (4%) gave opposite results in terms of flavor, delayed fermentation and weak texture with syneresis also. Conclusion: Therefore, it is recommended to use ulvan polysaccharide as prebiotic in the manufacture of synbiotic yogurt by adding 1-2%; and the flavor can be improved by adding any natural flavor to the synbiotic yogurt.
背景与目的:人们对食用海藻作为有益多糖的来源越来越感兴趣。Ulva lactuca是一种分布广泛的大型藻类,因其丰富的营养价值而被人类和牲畜大量食用。许多研究表明,乳酸菌的不同提取物具有一定的营养和生物学价值。Ulvan是其结构水溶性硫酸多糖之一。本研究的主要目的是利用不同比例的紫万多糖作为益生元成分,并利用益生菌发酵菌制作固定式合成酸奶。然后对合成酸奶的品质特性进行研究。方法:采用热水提取法和乙醇沉淀法提取枸杞多糖。以新鲜脱脂牛奶和ulvan多糖为益生元,分别添加1%、2%和4% (w/v)的浓度,制备了益生酸奶。将混合物加热到90°C 10分钟,然后立即冷却到39°-40°C;接种3%的益生菌发酵剂(含嗜酸乳杆菌LA-5、嗜热链球菌TH-4、双歧杆菌Bb-12)。将接种后的乳汁分装于100 mL塑料杯中,40℃孵育至完全凝固。将合成酸奶移入7°±1°C的冰箱中,保存9天;然后对不同处理进行保鲜和冷藏9 d后的细菌学、物理、化学和感官评价。结果:结果表明,添加1-2%的ulvan多糖制备的合成酸奶具有良好的化学和物理性能,并能促进益生菌的生长和活性。而高添加量(4%)的发酵效果则相反,发酵时间延长,口感变弱,且有增效作用。结论:因此,建议在合成酸奶中添加1-2%的ulvan多糖作为益生元;在合成酸奶中加入任何天然风味都可以改善酸奶的风味。
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引用次数: 16
The Probiotic BIOHM Improves Nutrient Absorption by Disrupting Gastrointestinal Biofilms 益生菌BIOHM通过破坏胃肠道生物膜改善营养吸收
Pub Date : 2019-01-01 DOI: 10.35248/2329-8901.19.7.213
M. Ghannoum, A. Ghannoum, C. Hager, M. Retuerto, N. Isham, T. McCormick
Aim: To demonstrate the ability of a probiotic supplement (BIOHM) to disrupt pathogenic gastrointestinal biofilms, thus enhancing nutrient absorption. Methods: A filter insert model using Caco-2 cells to mimic an intestinal monolayer was employed to determine the ability of BIOHM to disrupt a mixed species (Candida tropicalis, Escherichia coli and Serratia marcescens) biofilm known to occur in patients with Crohn’s disease. Penetration of vitamin C and casein as representative of vitamins and proteins, respectively, was then measured. Results: Application of BIOHM led to a significant increase in casein penetration through the Caco-2 cell epithelial monolayer in the absence of biofilms (p value<0.0001). Additionally, the combination of mixed species biofilms grown on a Caco-2 cell monolayer with BIOHM filtrate resulted in higher penetration of vitamin C and casein through the monolayer compared to untreated controls. Conclusion: Our in vitro data indicates that the combination of ingredients in the BIOHM probiotic may enhance nutrient permeability, thus leading to increased overall absorption of proteins and vitamins.
目的:证明益生菌补充剂(BIOHM)破坏致病性胃肠道生物膜的能力,从而促进营养吸收。方法:采用Caco-2细胞模拟肠道单层的过滤器插入模型来确定BIOHM破坏已知发生在克罗恩病患者中的混合物种(热带假丝酵母、大肠杆菌和粘质沙氏菌)生物膜的能力。然后分别测量作为维生素和蛋白质代表的维生素C和酪蛋白的渗透。结果:在没有生物膜的情况下,BIOHM的应用导致酪蛋白通过Caco-2细胞上皮单层的渗透显著增加(p值<0.0001)。此外,与未经处理的对照相比,在Caco-2细胞单层上生长的混合物种生物膜与BIOHM滤液的组合导致维生素C和酪蛋白通过单层的渗透率更高。结论:我们的体外实验数据表明,BIOHM益生菌中的成分组合可以增强营养物质的通透性,从而增加蛋白质和维生素的整体吸收。
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引用次数: 0
Assessment of Probiotic Properties of Some Bacteria Isolated from Dairy Products 乳制品中某些细菌益生菌特性的评价
Pub Date : 2019-01-01 DOI: 10.35248/2329-8901.19.7.207
Rahman Mr, Kabir Ms, Khan Zum, Pramanik Mk
Objectives: A study was undertaken to isolate bacteria from fermented dairy products and evaluate their probiotic potential. Methods: From the colonies formed on MRS agar plates, 8 bacterial isolates were selected and evaluated for their anti-microbial and cholesterol assimilation capability. Results: Among the tested isolates, P3 showed maximum antimicrobial activity against Staphylococcus aureus ATCC 6538 and P8 showed maximum antimicrobial activity against Salmonella enteritidis ATCC 13076, Listeria monocytogenes ATCC 7644 and Escherichia coli ATCC 35150. The isolate P8 also showed highest cholesterol assimilation capability (41.91%) which is followed by isolate P3 (35.95%). Based on the morphological, biochemical and physiological characteristics the isolate P3 and P8 were identified as Lactococcus lactis and Streptococcus thermophilus, respectively. Conclusion: The studied fermented dairy products available in Bangladesh contain adequate number of lactic acid bacteria and some of the bacterial isolates possess appreciable probiotic properties.
目的:研究从发酵乳制品中分离细菌并评估其益生菌潜力。方法:从MRS琼脂平板上形成的菌落中筛选出8株细菌,并对其抑菌和胆固醇同化能力进行评价。结果:P3对金黄色葡萄球菌ATCC 6538的抑菌活性最高,P8对肠炎沙门氏菌ATCC 13076、单核增生李斯特菌ATCC 7644和大肠杆菌ATCC 35150的抑菌活性最高。分离物P8对胆固醇的同化能力最高(41.91%),其次是分离物P3(35.95%)。根据形态、生化和生理特征鉴定分离物P3和P8分别为乳酸乳球菌和嗜热链球菌。结论:所研究的孟加拉国发酵乳制品中含有足够数量的乳酸菌,其中一些菌株具有明显的益生菌特性。
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引用次数: 0
Potential Using of Ulvan Polysaccharide from Ulva lactuca as a Prebiotic in Synbiotic Yogurt Production 乳Ulva Ulvan多糖作为益生元在合成酸奶生产中的潜在应用
Pub Date : 2019-01-01 DOI: 10.35248/2329-8901.7.1.208
Samah Shalaby M, Hadear Amin H
Background and objective: There is growing interesting for edible marine algae species as a source for beneficial polysaccharides. Ulva lactuca is a widespread macroalga and highly consumed by humans and livestock because of its great nutritional values. Many studies showed that different extracts of U. lactuca possess nutritional and biological values. Ulvan is one of its structural water-soluble sulfated polysaccharides. The main objective of this study is preparing synbiotic yogurt by using ulvan polysaccharide by different percentage as a prebiotic ingredient and using probiotic starter bacteria in the manufacture of set synbiotic yogurt. Then study the quality properties of the resultant synbiotic yogurt. Methodology: ulvan polysaccharide was extracted from Ulva lactuca using the methods of hot water-extraction and ethanol-precipitation. The synbiotic yogurt was prepared from fresh skim milk and ulvan polysaccharide as prebiotics, which was added by different concentrations: 1%, 2% and 4% (w/v). The mixtures were heated to 90°C for 10 min and immediately cooled to 39°-40°C; and inoculated with 3% of probiotic starter culture (containing of Lactobacillus acidophilus LA-5 Streptococcus thermophilus TH-4, and Bifidobacterium sp. Bb-12). The inoculated milks were distributed into 100 mL plastic cups and incubated at 40°C till complete coagulation. The synbiotic yogurt were transferred to the refrigerator at 7° ± 1°C and storage for 9 days; then the treatments were analyzed for the bacteriological, physical, chemical, and organoleptic assessments at fresh time and after 9 days of cold storage. Results: The results showed that adding of 1-2% of the ulvan polysaccharide presented synbiotic yogurt with good chemical and physical properties, as well as stimulated the growth and activity of probiotic bacteria. But the addition of high percentage (4%) gave opposite results in terms of flavor, delayed fermentation and weak texture with syneresis also. Conclusion: Therefore, it is recommended to use ulvan polysaccharide as prebiotic in the manufacture of synbiotic yogurt by adding 1-2%; and the flavor can be improved by adding any natural flavor to the synbiotic yogurt..
背景与目的:人们对食用海藻作为有益多糖的来源越来越感兴趣。Ulva lactuca是一种分布广泛的大型藻类,因其丰富的营养价值而被人类和牲畜大量食用。许多研究表明,乳酸菌的不同提取物具有一定的营养和生物学价值。Ulvan是其结构水溶性硫酸多糖之一。本研究的主要目的是利用不同比例的紫万多糖作为益生元成分,并利用益生菌发酵菌制作固定式合成酸奶。然后对合成酸奶的品质特性进行研究。方法:采用热水提取法和乙醇沉淀法提取枸杞多糖。以新鲜脱脂牛奶和ulvan多糖为益生元,分别添加1%、2%和4% (w/v)的浓度,制备了益生酸奶。将混合物加热到90°C 10分钟,然后立即冷却到39°-40°C;接种3%的益生菌发酵剂(含嗜酸乳杆菌LA-5、嗜热链球菌TH-4、双歧杆菌Bb-12)。将接种后的乳汁分装于100 mL塑料杯中,40℃孵育至完全凝固。将合成酸奶移入7°±1°C的冰箱中,保存9天;然后对不同处理进行保鲜和冷藏9 d后的细菌学、物理、化学和感官评价。结果:结果表明,添加1-2%的ulvan多糖制备的合成酸奶具有良好的化学和物理性能,并能促进益生菌的生长和活性。而高添加量(4%)的发酵效果则相反,发酵时间延长,口感变弱,且有增效作用。结论:因此,建议在合成酸奶中添加1-2%的ulvan多糖作为益生元;在合成酸奶中加入任何天然风味都可以改善酸奶的风味。
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引用次数: 8
The Probiotic BIOHM Improves Nutrient Absorption by Disrupting Gastrointestinal Biofilms 益生菌BIOHM通过破坏胃肠道生物膜改善营养吸收
Pub Date : 2019-01-01 DOI: 10.35248/2329-8901.7.2.213
G. M, Ghannoum A, Hager C, R. M, I. N, Mccormick Ts
{"title":"The Probiotic BIOHM Improves Nutrient Absorption by Disrupting Gastrointestinal Biofilms","authors":"G. M, Ghannoum A, Hager C, R. M, I. N, Mccormick Ts","doi":"10.35248/2329-8901.7.2.213","DOIUrl":"https://doi.org/10.35248/2329-8901.7.2.213","url":null,"abstract":"","PeriodicalId":16865,"journal":{"name":"Journal of Probiotics & Health","volume":"41 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88003756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Use of flow cytometry to assess the health of probiotic bacillus endospores 利用流式细胞术评估益生菌孢子内芽孢的健康状况
Pub Date : 2018-12-12 DOI: 10.4172/2329-8901-C2-030
pDana Buckmanp
{"title":"Use of flow cytometry to assess the health of probiotic bacillus endospores","authors":"pDana Buckmanp","doi":"10.4172/2329-8901-C2-030","DOIUrl":"https://doi.org/10.4172/2329-8901-C2-030","url":null,"abstract":"","PeriodicalId":16865,"journal":{"name":"Journal of Probiotics & Health","volume":"13 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75855370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Anti-obesity effect of kefir-derived exopolysaccharides 开菲尔衍生胞外多糖的抗肥胖作用
Pub Date : 2018-09-27 DOI: 10.4172/2329-8901-c1-027
pHyunsook Kimp
{"title":"Anti-obesity effect of kefir-derived exopolysaccharides","authors":"pHyunsook Kimp","doi":"10.4172/2329-8901-c1-027","DOIUrl":"https://doi.org/10.4172/2329-8901-c1-027","url":null,"abstract":"","PeriodicalId":16865,"journal":{"name":"Journal of Probiotics & Health","volume":"20 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82000505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Effects of Salinity on Acid Production and Growth of Three Probiotic Microbes with Potential for Application in Intensive Shrimp Aquaculture 盐度对三种具有集约化对虾养殖潜力的益生菌产酸及生长的影响
Pub Date : 2018-03-12 DOI: 10.4172/2329-8901.1000191
Gustavo Pinoargote, S. Ravishankar
Shrimp has been among the top value-added products targeted for production by the aquaculture industry. The increasing demand for shrimp has led to a massive increase in production in several countries across the world. Intensive and super-intensive production systems are facing great challenges handling newly emerging shrimp diseases. The use of antibiotics was one of the first approaches when dealing with such diseases, but the effects of misusing antibiotics and the appearance of antibiotic resistant bacteria are of public concern. As an alternative, probiotics have been applied in aquaculture systems to increase disease resistance, improve feed efficiency, maintain water quality and enhance the growth of aquatic organisms. In this study, the ability of three probiotic microbes to tolerate salinity levels commonly found in intensive shrimp production systems were evaluated. Lactobacillus casei, Saccharomyces cerevisiae and Rhodopseudomonas palustris were cultured in MRS broth, yeast and mold broth, and Van Neil’s broth, respectively, enriched with 1 and 2% NaCl. Microbial survival between treatments were compared as well as the metabolic activity in terms of acidity levels. Additionally, cell morphology was compared using scanning electron microscopy. L. casei and S. cerevisiae showed no significant differences (P>0.05) in media with 1% and 2% NaCl in terms of microbial survival and media acidity levels at 24 h. R. palustris showed a prolonged lag phase extending up to 12 h in 1% and 48 h in 2% NaCl media, and acidity of the media did not vary significantly. Cell morphology of all microbes did not change significantly across all treatments. From these results, it was concluded that L. casei, S. cerevisiae and R. palustris are suitable for application in aquaculture ponds with up to 2% salinity.
虾一直是水产养殖业生产的最高附加值产品之一。对虾的需求不断增加,导致世界上几个国家的产量大幅增加。集约化和超集约化生产系统在处理新出现的虾类疾病方面面临巨大挑战。抗生素的使用是处理这些疾病的最初方法之一,但滥用抗生素的影响和抗生素耐药细菌的出现是公众关注的问题。作为一种替代品,益生菌已被应用于水产养殖系统中,以增强抗病性、提高饲料效率、维持水质和促进水生生物的生长。在这项研究中,三种益生菌微生物对集约化虾生产系统中常见的盐度水平的耐受能力进行了评估。分别在MRS培养液、酵母和霉菌培养液和Van Neil’s培养液中培养干酪乳杆菌、酿酒酵母菌和palustris红假单胞菌。比较了不同处理之间的微生物存活率以及酸度水平方面的代谢活性。此外,用扫描电镜比较细胞形态。在1%和2% NaCl培养基中,干酪乳杆菌和酿酒酵母的微生物存活率和培养基酸度水平在24 h无显著差异(P>0.05), palustris在1%和2% NaCl培养基中表现出较长的滞后期,分别可达12 h和48 h,培养基酸度变化不显著。所有微生物的细胞形态在所有处理中都没有显著变化。结果表明,干酪乳杆菌、酿酒葡萄球菌和palustris适合在盐度不超过2%的养殖池塘中施用。
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引用次数: 1
Nutritional and Microbiological Evaluation of Ricebean (Vigna umbellata) Based Probiotic Food Multi Mix Using Lactobacillus plantarum and Lactobacillus rhamnosus 植物乳杆菌与鼠李糖乳杆菌复合益生菌复合食品的营养与微生物学评价
Pub Date : 2018-01-01 DOI: 10.4172/2329-8901.1000200
D. Baruah, M. Das, R. K. Sharma
The sustainable developmental goal emphasized a holistic approach to achieve zero hunger, good health and wellbeing till 2030. To achieve such goal, food diversifications like food fortification can be employed to prevent hunger, starvation and micronutrient deficiencies on a long term basis. One of the best food based approach to address these issues is by development of new value added products like Food Multi Mix from local functional ingredients for better affordability, accessibility and availability among vulnerable section of our community and also for reducing the major risk factors for noncommunicable diseases. WHO and FAO also focus on utilization of some underutilized legume or pulses in recent years. The use of microbial food additives in improving productivity of functional foods is currently generating a great deal of interest in scientific food industry. The objective of the study was to standardize ricebean based probiotic FMM and to evaluate its nutritional quality. Two FMM were developed namely FMM I and FMM II. All the ingredients were preprocessed before mixing together. FMM I was formulated based on energy density value between 1512.00-1890.00 kJ (360-450 kcal) per 100 g of sample and further by mixing all the ingredients at appropriate amount. Subsequently FMM II was formulated by inoculating probiotic bacteria viz Lactobacillus plantarum and Lactobacillus rhamnosus in FMM I both individually and in combination in different test samples. The test sample containing highest microbial viability after 30 days of storage was designated as FMM II. Both the Food Multi mixes had optimum macro and micronutrients. Results showed that probiotification played an important role in enhancing the nutrient content in FMM II. The Food Multi Mixes also had good physical properties in terms of bulk density, viscosity, water holding and fat holding capacity. Thus, the FMM concept had been an effective tool in developing food products from underutilized crops to bridge the gap between protein energy malnutrition in developing countries.
可持续发展目标强调采取综合办法,到2030年实现零饥饿、良好健康和福祉。为了实现这一目标,可以采用食物强化等食物多样化,以长期预防饥饿、饥饿和微量营养素缺乏。解决这些问题的最佳方法之一是以食品为基础,开发新的增值产品,如从当地功能成分中提取的多种食品,以提高我们社区弱势群体的可负担性、可及性和可获得性,并减少非传染性疾病的主要风险因素。近年来,世卫组织和粮农组织还注重利用一些未充分利用的豆类。利用微生物食品添加剂提高功能性食品的生产效率是目前科学食品工业的一大研究热点。本研究旨在对豆豉益生菌FMM进行标准化,并对其营养品质进行评价。开发了两种FMM,即FMM I和FMM II。所有的配料在混合之前都经过了预处理。FMM I的配方是基于每100 g样品的能量密度值在1512.00-1890.00 kJ (360-450 kcal)之间,并进一步将所有成分以适当的量混合。随后在不同的试验样品中分别接种植物乳杆菌和鼠李糖乳杆菌这两种益生菌配制FMM II。将贮藏30天后微生物活力最高的样品命名为FMM II。两种食品组合均具有最佳的宏量营养素和微量营养素。结果表明,益生菌对提高FMM II的营养成分含量具有重要作用。该食品复合混合物在容重、粘度、持水和持脂能力等方面也具有良好的物理性能。因此,FMM概念是利用未充分利用的作物开发粮食产品的有效工具,以弥合发展中国家蛋白质能量营养不良之间的差距。
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引用次数: 5
期刊
Journal of Probiotics & Health
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