Pub Date : 2017-09-20DOI: 10.4172/2329-8901.1000183
S. Hajare
Role of probiotics in regulation of immune system and development in microflora has begun to implicate in humans and animals. Inconsistent data exist regarding the effect of a single probiotic strain in malnutrate children. The aim of this study clinically proven Lactobacillus acidophilus strain LBKV-3 intended as probiotc for human was used to test its effect on modulation of immunoglobulin and composition of faecal microflora. To accomplish this task, 135 malnutrate children were orally administrated with buffalo milk as control, fresh curd as a special module for assessment of stimulatory effects and probiotic acidophilus milk used as a probiotic. During feeding, we evaluate the level of saliva IgA, serum IgG and IgE and faecal microflora. The result of our study demonstrated that the frequency of immunoglobulin and microflora was not affected when children consumed buffalo milk. In presence of fresh curd, the level of IgA, IgG and composition of friendly bacteria slightly increases and proportion of pathogenic bacteria declines. However, probiotic acidophilus milk significantly triggers the level of IgA and IgG as well as population of helpful bacteria rises and concentration of pathogenic bacteria declines in comparison to fresh curd. Irrespective of IgA and IgG, the ratio of IgE reduces in presence of fresh curd and probiotic acidophilus milk. Thus, our study showed that probiotic has positive effects on modulation of immunoglobulin and regulation of gut microflora. To confirm these findings, large-scale experiments involving the use of a large number of humans of different age groups and under different health and nutritional condition are required to be performed. Moreover, a different dose or strain of probiotic also warrants further confirmation.
{"title":"Oral Administration of LBKV-3 as Probiotic Enhances Immunoglobulin Level and Faecal Microflora in Malnutrate Children","authors":"S. Hajare","doi":"10.4172/2329-8901.1000183","DOIUrl":"https://doi.org/10.4172/2329-8901.1000183","url":null,"abstract":"Role of probiotics in regulation of immune system and development in microflora has begun to implicate in humans and animals. Inconsistent data exist regarding the effect of a single probiotic strain in malnutrate children. The aim of this study clinically proven Lactobacillus acidophilus strain LBKV-3 intended as probiotc for human was used to test its effect on modulation of immunoglobulin and composition of faecal microflora. To accomplish this task, 135 malnutrate children were orally administrated with buffalo milk as control, fresh curd as a special module for assessment of stimulatory effects and probiotic acidophilus milk used as a probiotic. During feeding, we evaluate the level of saliva IgA, serum IgG and IgE and faecal microflora. The result of our study demonstrated that the frequency of immunoglobulin and microflora was not affected when children consumed buffalo milk. In presence of fresh curd, the level of IgA, IgG and composition of friendly bacteria slightly increases and proportion of pathogenic bacteria declines. However, probiotic acidophilus milk significantly triggers the level of IgA and IgG as well as population of helpful bacteria rises and concentration of pathogenic bacteria declines in comparison to fresh curd. Irrespective of IgA and IgG, the ratio of IgE reduces in presence of fresh curd and probiotic acidophilus milk. Thus, our study showed that probiotic has positive effects on modulation of immunoglobulin and regulation of gut microflora. To confirm these findings, large-scale experiments involving the use of a large number of humans of different age groups and under different health and nutritional condition are required to be performed. Moreover, a different dose or strain of probiotic also warrants further confirmation.","PeriodicalId":16865,"journal":{"name":"Journal of Probiotics & Health","volume":"44 1","pages":"1-9"},"PeriodicalIF":0.0,"publicationDate":"2017-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73658481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-09-20DOI: 10.4172/2329-8901.1000182
De Marco Stefania, P. Mir, M. Diana, Zadra Claudia, Pagiotti Rita, Pietrella Donatella
Synbiotics are a combination of probiotic bacteria and the growth promoting prebiotic ingredients that purport “synergism”. Probiotics are well known for their ability to restore a healthful balance of commensal microbes supporting the repair of hyper-permeable epithelial barriers and interfering with infection. The present research was designed to evaluate the anti-virulence role of cell-free supernatants of synbiotic cultures. The effect of prebiotics fructo-oligosaccharide, inulin and isomaltose on probiotic Lactobacillus acidophilus, Lactococcus lactis, Lactobacillus casei, Lactobacillus reuteri and Saccharomyces boulardii growth, fermentation products has been investigated. Cell-free supernatants of synbiotics cultures have been studied for their antimicrobial, antibiofilm and anti-adhesion properties. Results highlight that prebiotics can enhance the antimicrobial and anti-virulence activities of probiotics against potential pathogens Staphylococcus aureus and Escherichia coli. The present research supports, for the first time, the value of anti-virulence activity of metabolites produced by synbiotics suggesting their use as a suitable adjuvant in antibacterial treatment.
{"title":"Antibiofilm and Antiadhesive Activities of Different Synbiotics","authors":"De Marco Stefania, P. Mir, M. Diana, Zadra Claudia, Pagiotti Rita, Pietrella Donatella","doi":"10.4172/2329-8901.1000182","DOIUrl":"https://doi.org/10.4172/2329-8901.1000182","url":null,"abstract":"Synbiotics are a combination of probiotic bacteria and the growth promoting prebiotic ingredients that purport “synergism”. Probiotics are well known for their ability to restore a healthful balance of commensal microbes supporting the repair of hyper-permeable epithelial barriers and interfering with infection. The present research was designed to evaluate the anti-virulence role of cell-free supernatants of synbiotic cultures. The effect of prebiotics fructo-oligosaccharide, inulin and isomaltose on probiotic Lactobacillus acidophilus, Lactococcus lactis, Lactobacillus casei, Lactobacillus reuteri and Saccharomyces boulardii growth, fermentation products has been investigated. Cell-free supernatants of synbiotics cultures have been studied for their antimicrobial, antibiofilm and anti-adhesion properties. Results highlight that prebiotics can enhance the antimicrobial and anti-virulence activities of probiotics against potential pathogens Staphylococcus aureus and Escherichia coli. The present research supports, for the first time, the value of anti-virulence activity of metabolites produced by synbiotics suggesting their use as a suitable adjuvant in antibacterial treatment.","PeriodicalId":16865,"journal":{"name":"Journal of Probiotics & Health","volume":"29 1","pages":"1-9"},"PeriodicalIF":0.0,"publicationDate":"2017-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81573976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-09-14DOI: 10.4172/2329-8901.1000181
A. Hoffmann, R. Daniels
Probiotics are defined as live microorganisms that beneficially affect the host. Probiotic bacteria have been used therapeutically for years to target gastrointestinal disease by rebalancing the complex microflora. Besides the gastrointestinal tract also the oral cavity is highly colonized by bacteria and many different bacterial species are part of the microbiota in the mouth, as it offers ideal conditions for bacteria with a stable temperature, moist surface with a relatively stable pH and regular supply of nutrients. By disturbing the balance of microorganisms in the oral cavity or by extensive accumulation of plaque, the ratio of pathogenic organisms can increase and lead to oral health problems. Probiotic bacteria, like lactobacilli, are a promising treatment strategy for oral diseases with a microbiological aetiology. Those include plaqueA¢Â€Âassociated diseases like dental caries, which is an infectious disease with microbial processes eroding and destroying the hard dental tissue or inflammation of periodontal tissue, namely gingivitis and the more severe periodontitis. Moreover, endodontic infections, and even fungal, viral and acute bacterial infections could be treated by a probiotic therapy. The interest of probiotics in the field of oral health is growing, although it is still in its infancy. The present review adresses criteria for the selection of probiotic lactobacilli strains. It encompasses existing evidence on the use of lactobacilli for caries, halitosis and candidiasis, as well as for periodontal disease like Gingivitis and periodontitis.
{"title":"Lactobacilli for the Treatment of Oral Diseases","authors":"A. Hoffmann, R. Daniels","doi":"10.4172/2329-8901.1000181","DOIUrl":"https://doi.org/10.4172/2329-8901.1000181","url":null,"abstract":"Probiotics are defined as live microorganisms that beneficially affect the host. Probiotic bacteria have been used therapeutically for years to target gastrointestinal disease by rebalancing the complex microflora. Besides the gastrointestinal tract also the oral cavity is highly colonized by bacteria and many different bacterial species are part of the microbiota in the mouth, as it offers ideal conditions for bacteria with a stable temperature, moist surface with a relatively stable pH and regular supply of nutrients. By disturbing the balance of microorganisms in the oral cavity or by extensive accumulation of plaque, the ratio of pathogenic organisms can increase and lead to oral health problems. Probiotic bacteria, like lactobacilli, are a promising treatment strategy for oral diseases with a microbiological aetiology. Those include plaqueA¢Â€Âassociated diseases like dental caries, which is an infectious disease with microbial processes eroding and destroying the hard dental tissue or inflammation of periodontal tissue, namely gingivitis and the more severe periodontitis. Moreover, endodontic infections, and even fungal, viral and acute bacterial infections could be treated by a probiotic therapy. The interest of probiotics in the field of oral health is growing, although it is still in its infancy. The present review adresses criteria for the selection of probiotic lactobacilli strains. It encompasses existing evidence on the use of lactobacilli for caries, halitosis and candidiasis, as well as for periodontal disease like Gingivitis and periodontitis.","PeriodicalId":16865,"journal":{"name":"Journal of Probiotics & Health","volume":"54 1","pages":"1-6"},"PeriodicalIF":0.0,"publicationDate":"2017-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83056768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-09-06DOI: 10.4172/2329-8901.1000176
K. Akram, Tajabadi Ali, R. M. Hassan, Tadayonfar Moosaalreza
Introduction: The role of inflammation and malnutrition in critically ill patients has been shown in some studies. The aim of the study is to determine the effect of symbiotic on serum systemic inflammatory marker (hs-CRP) and albumin in critically ill patients. Methods: Sixty patients admitted to the intensive care unit of Sabzevar Vasei hospital were randomized two groups that received symbiotic or placebo for 2 weeks. Levels of serum hs-CRP and albumin were measured before and after the study. Results: There was a significant differences between two groups the Levels of serum hs-CRP (P=0.0001) and albumin (P=0.0001). Conclusion: Results of study were showed that administration of symbiotic in critically ill patients reduced the levels of serum hs-CRP and increased the levels of serum albumin.
{"title":"Effect of Synbiotic Supplementation on Serum Systemic Inflammatory Marker and Serum Albumin in Patients Admitted to ICU","authors":"K. Akram, Tajabadi Ali, R. M. Hassan, Tadayonfar Moosaalreza","doi":"10.4172/2329-8901.1000176","DOIUrl":"https://doi.org/10.4172/2329-8901.1000176","url":null,"abstract":"Introduction: The role of inflammation and malnutrition in critically ill patients has been shown in some studies. The aim of the study is to determine the effect of symbiotic on serum systemic inflammatory marker (hs-CRP) and albumin in critically ill patients. \u0000Methods: Sixty patients admitted to the intensive care unit of Sabzevar Vasei hospital were randomized two groups that received symbiotic or placebo for 2 weeks. Levels of serum hs-CRP and albumin were measured before and after the study. \u0000Results: There was a significant differences between two groups the Levels of serum hs-CRP (P=0.0001) and albumin (P=0.0001). \u0000Conclusion: Results of study were showed that administration of symbiotic in critically ill patients reduced the levels of serum hs-CRP and increased the levels of serum albumin.","PeriodicalId":16865,"journal":{"name":"Journal of Probiotics & Health","volume":"1 1","pages":"1-2"},"PeriodicalIF":0.0,"publicationDate":"2017-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80224544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-08-30DOI: 10.4172/2329-8901.1000180
J. Samot, H. Belkhelfa, L. Haddioui, C. Badet
Bacterial competitiveness appears to be an efficient way to fight pathogenic oral flora. This competition may be facilitated by probiotics, particularly in periodontal diseases. The aim of this study was to investigate the probiotic properties of 61 clinical isolates of oral lactobacilli. The inhibitory activity of the tested strains against periodontopathogens was assessed with the agar overlay technique. The results obtained, as well as those resulting from previous work, led us to select the nine most promising strains on which we conducted further tests, such as evaluating their coaggregation capacities with various oral species and the production of proinflammatory cytokines by PBMC. We also evaluated the safety of the probiotics by assessing their sensitivity to antibiotics. Their possible involvement in halitosis was investigated by testing their ability to produce volatile sulfur compounds. The results of the agar overlay technique showed that all the lactobacilli strains had an antibacterial activity against Tanerella forsythia, Treponema denticola, and Aggregatibacter actinomycetemcomitans. Fifty-two strains slightly inhibited the growth of P gingivalis and only two had no activity on F. nucleatum. The nine strains tested did not coaggregate with P gingivalis, T forsythia, T denticola or A actinomycetemcomitans. Three strains strongly coaggregated with F nucleatum. Only three strains produced lower IL-6 than the activator at the maximum concentration tested in this study. However, none of the 9 strains produced a greater amount of IL-8 than that obtained with 1 μg/ml of LPS. These results show us that all the strains were sensitive to the antibiotics tested, except for one strain which showed resistance to penicillin. The production of CSV by the strains remained below the threshold of halitosis. Among the 61 strains tested, 9 proved to be of interest including one that was particularly promising.
{"title":"Probiotic Properties of Lactobacilli that could be Used Against Periodontitis","authors":"J. Samot, H. Belkhelfa, L. Haddioui, C. Badet","doi":"10.4172/2329-8901.1000180","DOIUrl":"https://doi.org/10.4172/2329-8901.1000180","url":null,"abstract":"Bacterial competitiveness appears to be an efficient way to fight pathogenic oral flora. This competition may be facilitated by probiotics, particularly in periodontal diseases. The aim of this study was to investigate the probiotic properties of 61 clinical isolates of oral lactobacilli. The inhibitory activity of the tested strains against periodontopathogens was assessed with the agar overlay technique. The results obtained, as well as those resulting from previous work, led us to select the nine most promising strains on which we conducted further tests, such as evaluating their coaggregation capacities with various oral species and the production of proinflammatory cytokines by PBMC. We also evaluated the safety of the probiotics by assessing their sensitivity to antibiotics. Their possible involvement in halitosis was investigated by testing their ability to produce volatile sulfur compounds. The results of the agar overlay technique showed that all the lactobacilli strains had an antibacterial activity against Tanerella forsythia, Treponema denticola, and Aggregatibacter actinomycetemcomitans. Fifty-two strains slightly inhibited the growth of P gingivalis and only two had no activity on F. nucleatum. The nine strains tested did not coaggregate with P gingivalis, T forsythia, T denticola or A actinomycetemcomitans. Three strains strongly coaggregated with F nucleatum. Only three strains produced lower IL-6 than the activator at the maximum concentration tested in this study. However, none of the 9 strains produced a greater amount of IL-8 than that obtained with 1 μg/ml of LPS. These results show us that all the strains were sensitive to the antibiotics tested, except for one strain which showed resistance to penicillin. The production of CSV by the strains remained below the threshold of halitosis. Among the 61 strains tested, 9 proved to be of interest including one that was particularly promising.","PeriodicalId":16865,"journal":{"name":"Journal of Probiotics & Health","volume":"15 1","pages":"1-6"},"PeriodicalIF":0.0,"publicationDate":"2017-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89162170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-08-16DOI: 10.4172/2329-8901.1000179
Rajesh Gopal, S. Elumalai
The use of chemical advances to mechanical research, improvement, and assembling has turned into a critical field. Since the creation of rough rennet in 1874, a few catalysts have been marketed, and utilized for restorative, supplementary, and different applications. Late headways in biotechnology now enable organizations to create more secure and more affordable chemicals with upgraded intensity and specificity. Cancer prevention agent catalysts are developing as another expansion to the pool of modern chemicals and are outperforming every single other compound as far as the volume of research and creation. In the 1990s, a cell reinforcement chemical-superoxide dismutase (SOD) was brought into the market. In spite of the fact that the catalyst at first demonstrated extraordinary guarantee in restorative applications, it didn't perform up to desires. Therefore, its utilization was restricted to nontranquilize applications in people and medication applications in creatures. This survey compresses the ascent and fall of SOD at the mechanical level, the purposes behind this, and potential future push territories that should be tended to. The audit likewise concentrates on other modernly significant parts of SOD, for example, mechanical significance, catalyst designing, generation procedures, and process streamlining and scale-up.
{"title":"Industrial Production of Superoxide Dismutase (SOD): A Mini Review","authors":"Rajesh Gopal, S. Elumalai","doi":"10.4172/2329-8901.1000179","DOIUrl":"https://doi.org/10.4172/2329-8901.1000179","url":null,"abstract":"The use of chemical advances to mechanical research, improvement, and assembling has turned into a critical field. Since the creation of rough rennet in 1874, a few catalysts have been marketed, and utilized for restorative, supplementary, and different applications. Late headways in biotechnology now enable organizations to create more secure and more affordable chemicals with upgraded intensity and specificity. Cancer prevention agent catalysts are developing as another expansion to the pool of modern chemicals and are outperforming every single other compound as far as the volume of research and creation. In the 1990s, a cell reinforcement chemical-superoxide dismutase (SOD) was brought into the market. In spite of the fact that the catalyst at first demonstrated extraordinary guarantee in restorative applications, it didn't perform up to desires. Therefore, its utilization was restricted to nontranquilize applications in people and medication applications in creatures. This survey compresses the ascent and fall of SOD at the mechanical level, the purposes behind this, and potential future push territories that should be tended to. The audit likewise concentrates on other modernly significant parts of SOD, for example, mechanical significance, catalyst designing, generation procedures, and process streamlining and scale-up.","PeriodicalId":16865,"journal":{"name":"Journal of Probiotics & Health","volume":"1 1","pages":"1-5"},"PeriodicalIF":0.0,"publicationDate":"2017-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91018685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-06-16DOI: 10.4172/2329-8901.1000178
Tai-Hwa Shih, Jengtung Chiang, Hung-Yi Wu, W. Tu, Cheng-Ta Tsai, S. Kuo, Chang-Young Fei
This study evaluated landscape characteristics associated with the incidence of ferret badger (Melogale moschata) rabies in the East Epidemic Area, Taiwan, where the disease has been endemic from 2013 till date. This study included 268 cases of ferret badger rabies reported from 59 townships during 2013-2016. A multivariable logistic regression analysis revealed that for townships with elevation between 0 and 200 m, or between 201 and 600 m, the estimated probability of the incidence of ferret badger rabies for each township increases with the rise of forest area. For townships with elevation higher than 600 m, the association is negative, a fact reflected by the interaction effect between variables of forest and elevation fitted in the model. This model provided by the combination of different land use types, human population density and elevation measures of townships, may be used in estimating the incidence probability of prioritizing areas for ferret badger rabies control on oral vaccination.
{"title":"The Landscape and Demographic Features Associated with Ferret Badger Rabies in Taiwan East Area","authors":"Tai-Hwa Shih, Jengtung Chiang, Hung-Yi Wu, W. Tu, Cheng-Ta Tsai, S. Kuo, Chang-Young Fei","doi":"10.4172/2329-8901.1000178","DOIUrl":"https://doi.org/10.4172/2329-8901.1000178","url":null,"abstract":"This study evaluated landscape characteristics associated with the incidence of ferret badger (Melogale moschata) rabies in the East Epidemic Area, Taiwan, where the disease has been endemic from 2013 till date. This study included 268 cases of ferret badger rabies reported from 59 townships during 2013-2016. A multivariable logistic regression analysis revealed that for townships with elevation between 0 and 200 m, or between 201 and 600 m, the estimated probability of the incidence of ferret badger rabies for each township increases with the rise of forest area. For townships with elevation higher than 600 m, the association is negative, a fact reflected by the interaction effect between variables of forest and elevation fitted in the model. This model provided by the combination of different land use types, human population density and elevation measures of townships, may be used in estimating the incidence probability of prioritizing areas for ferret badger rabies control on oral vaccination.","PeriodicalId":16865,"journal":{"name":"Journal of Probiotics & Health","volume":"21 1","pages":"1-5"},"PeriodicalIF":0.0,"publicationDate":"2017-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87526251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-06-13DOI: 10.4172/2329-8901.1000177
L. Angiolillo, A. Danza, A. Conte, D. Ma
In this study, the suitability of fish as substrate to be fortified by a probiotic bacterial strain was investigated. Lactobacillus rhamnosus GG (LGG) was used to the aim of the work. The study was first focused on the optimization of LGG microencapsulation by water-in-oil emulsion technique to avoid bacterial viability loss during cooking. Once the best microencapsulation conditions were individuated, the amount of microencapsulated probiotic to be added to the burger was increased to assure desired levels of viable LGG in the cooked fish burger. To assess the efficiency of the adopted fortification method, acid lactic bacterial count was monitored during every step of microcapsules preparation process as well as in both cooked and uncooked enriched fish samples. In order to evaluate the final acceptance of the fortified burger, the sensory properties of fortified fish burger were also assessed. Results demonstrated that proper microencapsulation conditions together with appropriate concentration of microencapsulated LGG into fish formulation would allow realizing probiotic-fortified burgers, also prized from a microbiological and sensory point of view with a consequent longer shelf life.
{"title":"How to Fortify a Fish Burger with Probiotic Microorganisms","authors":"L. Angiolillo, A. Danza, A. Conte, D. Ma","doi":"10.4172/2329-8901.1000177","DOIUrl":"https://doi.org/10.4172/2329-8901.1000177","url":null,"abstract":"In this study, the suitability of fish as substrate to be fortified by a probiotic bacterial strain was investigated. Lactobacillus rhamnosus GG (LGG) was used to the aim of the work. The study was first focused on the optimization of LGG microencapsulation by water-in-oil emulsion technique to avoid bacterial viability loss during cooking. Once the best microencapsulation conditions were individuated, the amount of microencapsulated probiotic to be added to the burger was increased to assure desired levels of viable LGG in the cooked fish burger. To assess the efficiency of the adopted fortification method, acid lactic bacterial count was monitored during every step of microcapsules preparation process as well as in both cooked and uncooked enriched fish samples. In order to evaluate the final acceptance of the fortified burger, the sensory properties of fortified fish burger were also assessed. Results demonstrated that proper microencapsulation conditions together with appropriate concentration of microencapsulated LGG into fish formulation would allow realizing probiotic-fortified burgers, also prized from a microbiological and sensory point of view with a consequent longer shelf life.","PeriodicalId":16865,"journal":{"name":"Journal of Probiotics & Health","volume":"96 1","pages":"1-7"},"PeriodicalIF":0.0,"publicationDate":"2017-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80819478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-06-05DOI: 10.4172/2329-8901.1000175
M. Talebi, M. Eshaghi, M. Bibalan, M. Rohani, M. Pourshafie
Background: Nowadays using of Bifidobacteria as a probiotic candidate for microbiome engineering and developing of human gut immunity have been considered. Given that many previous studies have emphasized probiotic properties are strain specific and exposing to stress shock such as low pH can be effective on probiotic criteria, so this study was performed for assessment and comparasion of antagonist activity of native Bifidobacterium with similar genotype homolgy isolated from paired mothers milk and infants feces. Methods: In this study, 56 mother’s milk and paired-infant feces were taken from rural area. Single colonies were picked up and confirmed by phenotypic and molecular identification. Randomly amplified polymorphic DNA (RAPD- PCR) and antibacterial activity carried out on selected strains. Results: Amongst all 56 samples, 31 samples, including 20 stools and 11 milks were positive for Bifidobacterium including B. breve, B. longum, B. bifidum. 12 of all strains were shared between stool and milk, including 6 Bifidobacterium longum, 4 B. breve and 2 B. bifidum. Between these 12 strains, only three strains of Bifidobacterium longum presented 100% similarity in RAPD- PCR analysis and antibacterial activity were strains specific and no similar activity were observed. Conclusion: In short, amongst all 56 paired infants stools and mothers milks, 12 different shared Bifidobacteria were isolated and identified that showed strain specific antagonist activity and some of these isolates possibly produced bacteriocin like proteins.
{"title":"Determination and Comparasion of Antibacterial Activity of NativeBifidobacterium Strains with Similar RAPD Profile Isolated from PairedInfants Stool and Mothers Milk","authors":"M. Talebi, M. Eshaghi, M. Bibalan, M. Rohani, M. Pourshafie","doi":"10.4172/2329-8901.1000175","DOIUrl":"https://doi.org/10.4172/2329-8901.1000175","url":null,"abstract":"Background: Nowadays using of Bifidobacteria as a probiotic candidate for microbiome engineering and developing of human gut immunity have been considered. Given that many previous studies have emphasized probiotic properties are strain specific and exposing to stress shock such as low pH can be effective on probiotic criteria, so this study was performed for assessment and comparasion of antagonist activity of native Bifidobacterium with similar genotype homolgy isolated from paired mothers milk and infants feces. \u0000Methods: In this study, 56 mother’s milk and paired-infant feces were taken from rural area. Single colonies were picked up and confirmed by phenotypic and molecular identification. Randomly amplified polymorphic DNA (RAPD- PCR) and antibacterial activity carried out on selected strains. \u0000Results: Amongst all 56 samples, 31 samples, including 20 stools and 11 milks were positive for Bifidobacterium including B. breve, B. longum, B. bifidum. 12 of all strains were shared between stool and milk, including 6 Bifidobacterium longum, 4 B. breve and 2 B. bifidum. Between these 12 strains, only three strains of Bifidobacterium longum presented 100% similarity in RAPD- PCR analysis and antibacterial activity were strains specific and no similar activity were observed. \u0000Conclusion: In short, amongst all 56 paired infants stools and mothers milks, 12 different shared Bifidobacteria were isolated and identified that showed strain specific antagonist activity and some of these isolates possibly produced bacteriocin like proteins.","PeriodicalId":16865,"journal":{"name":"Journal of Probiotics & Health","volume":"31 1","pages":"1-5"},"PeriodicalIF":0.0,"publicationDate":"2017-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82273061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-05-26DOI: 10.4172/2329-8901.1000174
Ro Bs, Lorella Paparo Bs, Rita Nocerino Rn, Carmen di Scala Ldn, Gaetano Polito Mlt, R. B. Canani
Lactobacillus rhamnosus GG survives in adequate amount after reconstitution of infant formulas according to the Food and Agriculture Organisation (FAO) and World Health Organisation (WHO) recommendations. Our results suggest that an infant formula containing this probiotic could be efficiently used in Countries where FAO/ WHO recommendations are mandatory.
{"title":"Evaluation of Lactobacillus rhamnosus GG Heat-Stability During Infant Formula Preparation","authors":"Ro Bs, Lorella Paparo Bs, Rita Nocerino Rn, Carmen di Scala Ldn, Gaetano Polito Mlt, R. B. Canani","doi":"10.4172/2329-8901.1000174","DOIUrl":"https://doi.org/10.4172/2329-8901.1000174","url":null,"abstract":"Lactobacillus rhamnosus GG survives in adequate amount after reconstitution of infant formulas according to the Food and Agriculture Organisation (FAO) and World Health Organisation (WHO) recommendations. Our results suggest that an infant formula containing this probiotic could be efficiently used in Countries where FAO/ WHO recommendations are mandatory.","PeriodicalId":16865,"journal":{"name":"Journal of Probiotics & Health","volume":"4 1","pages":"1-2"},"PeriodicalIF":0.0,"publicationDate":"2017-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72936917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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