Pub Date : 2018-01-01DOI: 10.4172/2329-8901.1000198
S. Naseem, A. King
{"title":"Effect of Lactobacilli on Production, Selected Compounds in Blood and Liver, and Manure of Laying Hens","authors":"S. Naseem, A. King","doi":"10.4172/2329-8901.1000198","DOIUrl":"https://doi.org/10.4172/2329-8901.1000198","url":null,"abstract":"","PeriodicalId":16865,"journal":{"name":"Journal of Probiotics & Health","volume":"59 1","pages":"1-11"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84613068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-01-01DOI: 10.4172/2329-8901.1000194
S. Raghuwanshi, Swati Misra, Rohit Sharma, P. Bisen
The advent of various health care policies and digital revolution has fuelled interest in the direction of food supplements, and as preventive or curative drug containing live non-pathogenic bacteria, probiotics. The widespread usage of probiotics by consumers and in clinical practice has emphatically made to understand the benefits associated with these products. The probiotic products are now being marketed worldwide by several multinational companies. Present review attempts to overview the health benefits of probiotics in impeding various lifestyle or metabolic associated disorders, clinical significance, their efficacy and influence on immune system.
{"title":"Probiotics: Nutritional Therapeutic Tool","authors":"S. Raghuwanshi, Swati Misra, Rohit Sharma, P. Bisen","doi":"10.4172/2329-8901.1000194","DOIUrl":"https://doi.org/10.4172/2329-8901.1000194","url":null,"abstract":"The advent of various health care policies and digital revolution has fuelled interest in the direction of food supplements, and as preventive or curative drug containing live non-pathogenic bacteria, probiotics. The widespread usage of probiotics by consumers and in clinical practice has emphatically made to understand the benefits associated with these products. The probiotic products are now being marketed worldwide by several multinational companies. Present review attempts to overview the health benefits of probiotics in impeding various lifestyle or metabolic associated disorders, clinical significance, their efficacy and influence on immune system.","PeriodicalId":16865,"journal":{"name":"Journal of Probiotics & Health","volume":"40 1","pages":"1-8"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74687582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-01-01DOI: 10.4172/2329-8901.1000197
S. Naseem, A. King
{"title":"Effects of Multi-Strain Lactobacillus and Sunflower Seed Meal on Nitrogen containing Compounds in Laying Hens Manure and Biological Components in Blood Serum","authors":"S. Naseem, A. King","doi":"10.4172/2329-8901.1000197","DOIUrl":"https://doi.org/10.4172/2329-8901.1000197","url":null,"abstract":"","PeriodicalId":16865,"journal":{"name":"Journal of Probiotics & Health","volume":"136 1","pages":"1-10"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73470317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-01-01DOI: 10.4172/2329-8901.1000199
T. Jayampathi, S. Jayatilake
Probiotics are live microorganisms that benefit a host by sustaining healthy micro flora in the gut. However several factors including pH, metabolites and dissolved oxygen limit the growth and survival of probiotic organisms. Prebiotics are selectively fermented ingredients that may increase the survival and activity of probiotics. Arrowroot is rich in prebiotics and may increase the biomass of probiotics. This study was aimed at determining the prebiotic potential of water extractable carbohydrates of local yam arrowroot. Arrowroot carbohydrates were extracted by wet milling and recovery was 14.32% (w/w). The results of the proximate analysis revealed that the percentages of moisture, ash, protein and fat were 10.15%, 4.91%, 0.13% and 0.32% respectively. The contents of starch and low molecular weight carbohydrates were 79.65% and 1.93% respectively. No reducing sugars were detected. 12% (w/v) of arrowroot carbohydrates were incorporated into 12% skim milk and distilled water along with an inoculum of 109 colony forming units (CFU)/g of Lactobacillus acidophilus in order to test the growth performances. 12% skim milk was used as a control. Treatments were incubated at 37°C anaerobically for 12 h followed by storage at 4°C for 14 days. The pH, titrable acidity and colony counts of L. acidophilus were determined throughout the storage period. Media added with arrowroot gave a significantly (p<0.05) higher pH compared to the control although there was no significant difference (p>0.05) among the two media where arrowroot was added. Similar results were observed for titrable acidity as well. Results showed that arrowroot carbohydrates significantly (p<0.05) increased the CFU (log 10)/g of L. acidophilus compared to the control (6.71) in both distilled water (9.34) and skim milk (9.01) media. It was revealed that water extractable arrowroot carbohydrates shows significant prebiotic effect by maintaining a higher viability of probiotics.
{"title":"Arrowroot ( Maranta arundinacea ) Extract Increases the Survival of Probiotic Lactobacillus acidophilus","authors":"T. Jayampathi, S. Jayatilake","doi":"10.4172/2329-8901.1000199","DOIUrl":"https://doi.org/10.4172/2329-8901.1000199","url":null,"abstract":"Probiotics are live microorganisms that benefit a host by sustaining healthy micro flora in the gut. However several factors including pH, metabolites and dissolved oxygen limit the growth and survival of probiotic organisms. Prebiotics are selectively fermented ingredients that may increase the survival and activity of probiotics. Arrowroot is rich in prebiotics and may increase the biomass of probiotics. This study was aimed at determining the prebiotic potential of water extractable carbohydrates of local yam arrowroot. Arrowroot carbohydrates were extracted by wet milling and recovery was 14.32% (w/w). The results of the proximate analysis revealed that the percentages of moisture, ash, protein and fat were 10.15%, 4.91%, 0.13% and 0.32% respectively. The contents of starch and low molecular weight carbohydrates were 79.65% and 1.93% respectively. No reducing sugars were detected. 12% (w/v) of arrowroot carbohydrates were incorporated into 12% skim milk and distilled water along with an inoculum of 109 colony forming units (CFU)/g of Lactobacillus acidophilus in order to test the growth performances. 12% skim milk was used as a control. Treatments were incubated at 37°C anaerobically for 12 h followed by storage at 4°C for 14 days. The pH, titrable acidity and colony counts of L. acidophilus were determined throughout the storage period. Media added with arrowroot gave a significantly (p<0.05) higher pH compared to the control although there was no significant difference (p>0.05) among the two media where arrowroot was added. Similar results were observed for titrable acidity as well. Results showed that arrowroot carbohydrates significantly (p<0.05) increased the CFU (log 10)/g of L. acidophilus compared to the control (6.71) in both distilled water (9.34) and skim milk (9.01) media. It was revealed that water extractable arrowroot carbohydrates shows significant prebiotic effect by maintaining a higher viability of probiotics.","PeriodicalId":16865,"journal":{"name":"Journal of Probiotics & Health","volume":"93 1","pages":"1-5"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80240515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-01-01DOI: 10.4172/2329-8901.1000196
Vincent Oko Obinna
Clostridium difficile (C. difficile) is an opportunistic pathogen. It takes advantage of the aged, children and the immune-compromised people and has caused severe attacks that have led to several deaths in the UK and the USA. This is common with in-patients and could even be contracted through medical personnel. Multi-drug resistance by pathogenic microbes resulted in the prevalence C. diff. This review discovered that antibiotic resistance by patience could be traced to what we eat especially beef, milk, fish, and poultry products from animals that had earlier been placed on antibiotics. This review seeks an alternative since the antibiotic war on pathogens is failing to, rescue human race from this catastrophe. Probiotics is veritable replacement for antibiotics. Also, adequate and regular sanitisations of the hospital premises and more careful attention to personal hygiene by the personnel could be helpful in preventing its spread. Patients fed with probiotics showed better recovery and those that received more regular supplies of probiotics showed quicker recovery and improved health. Probiotics, therefore, is a panacea to the Clostridium difficle associated diseases (CDAD) insurgence.
{"title":"Clostridium difficile and Probiotics","authors":"Vincent Oko Obinna","doi":"10.4172/2329-8901.1000196","DOIUrl":"https://doi.org/10.4172/2329-8901.1000196","url":null,"abstract":"Clostridium difficile (C. difficile) is an opportunistic pathogen. It takes advantage of the aged, children and the immune-compromised people and has caused severe attacks that have led to several deaths in the UK and the USA. This is common with in-patients and could even be contracted through medical personnel. Multi-drug resistance by pathogenic microbes resulted in the prevalence C. diff. This review discovered that antibiotic resistance by patience could be traced to what we eat especially beef, milk, fish, and poultry products from animals that had earlier been placed on antibiotics. This review seeks an alternative since the antibiotic war on pathogens is failing to, rescue human race from this catastrophe. Probiotics is veritable replacement for antibiotics. Also, adequate and regular sanitisations of the hospital premises and more careful attention to personal hygiene by the personnel could be helpful in preventing its spread. Patients fed with probiotics showed better recovery and those that received more regular supplies of probiotics showed quicker recovery and improved health. Probiotics, therefore, is a panacea to the Clostridium difficle associated diseases (CDAD) insurgence.","PeriodicalId":16865,"journal":{"name":"Journal of Probiotics & Health","volume":"4 1","pages":"1-5"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88124661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-12-21DOI: 10.4172/2329-8901.1000190
Objective: Probiotics have been associated with many health benefits and have an established safety record. The purpose of this pilot study was to determine the safety and efficacy of inactivated Bacillus coagulans GBI-30, 6086 (Staimune®) in supporting the immune system at rest, in response to an in vitro bacterial challenge, and in response to strenuous exercise. Methods: In a randomized double-blind placebo-controlled pilot study, 16 healthy adults received either Staimune® or Placebo over 28-d. Basal immune system was assessed (salivary IgA, complete blood count with differential, helper T-cell (CD3+/CD4+), cytotoxic T-cells (CD3+/CD8+), and natural killer cells (CD3-/56+ or CD3-/16+/56+) percentages/proportions. In addition, cytokine (IL-1b, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12 (p70), IL-13, IFN gamma, GM-CSF, and TNF-a) in response to an in vitro lipopolysaccharide challenge as well examining basal serum cortisol values. Subjects performed 60-min treadmill running at 60-80% of their Karvonen predicted max heart rate. Blood and salivary samples were also collected at 10, 60, and 120-min post-exercise. Data analysis was based upon changes over time relative to baseline for within group changes as well as between-group differences (P<0.05), 95% CI. Results: Both conditions supported mucosal immunity. Ten minutes after exercise WBC was significantly greater in the Staimune group vs. Placebo, no difference between groups at 60 or 120-min. Neutrophils and Monocytes were significantly greater in the Study Product group. There were variable impacts on eosinophils and basophils. Markers of non-specific and innate immunity were significantly impacted in Staimune® as were the impacts on pro and anti-inflammatory cytokines. There was significantly enhanced recovery for cortisol in the Staimune® group in the post-exercise period. Conclusion: Inactivated Bacillus coagulans GBI-30, 6086 supports the immune system at rest and after strenuous exercise. The study product may lessen the window of immune system suppression post-exercise, and enhance stress recovery supporting immunity.
{"title":"Inactivated Probiotic Bacillus coagulans GBI-30 Demonstrates Immunosupportive Properties in Healthy Adults Following Stressful Exercise","authors":"","doi":"10.4172/2329-8901.1000190","DOIUrl":"https://doi.org/10.4172/2329-8901.1000190","url":null,"abstract":"Objective: Probiotics have been associated with many health benefits and have an established safety record. The purpose of this pilot study was to determine the safety and efficacy of inactivated Bacillus coagulans GBI-30, 6086 (Staimune®) in supporting the immune system at rest, in response to an in vitro bacterial challenge, and in response to strenuous exercise. \u0000Methods: In a randomized double-blind placebo-controlled pilot study, 16 healthy adults received either Staimune® or Placebo over 28-d. Basal immune system was assessed (salivary IgA, complete blood count with differential, helper T-cell (CD3+/CD4+), cytotoxic T-cells (CD3+/CD8+), and natural killer cells (CD3-/56+ or CD3-/16+/56+) percentages/proportions. In addition, cytokine (IL-1b, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12 (p70), IL-13, IFN gamma, GM-CSF, and TNF-a) in response to an in vitro lipopolysaccharide challenge as well examining basal serum cortisol values. Subjects performed 60-min treadmill running at 60-80% of their Karvonen predicted max heart rate. Blood and salivary samples were also collected at 10, 60, and 120-min post-exercise. Data analysis was based upon changes over time relative to baseline for within group changes as well as between-group differences (P<0.05), 95% CI. \u0000Results: Both conditions supported mucosal immunity. Ten minutes after exercise WBC was significantly greater in the Staimune group vs. Placebo, no difference between groups at 60 or 120-min. Neutrophils and Monocytes were significantly greater in the Study Product group. There were variable impacts on eosinophils and basophils. Markers of non-specific and innate immunity were significantly impacted in Staimune® as were the impacts on pro and anti-inflammatory cytokines. There was significantly enhanced recovery for cortisol in the Staimune® group in the post-exercise period. \u0000Conclusion: Inactivated Bacillus coagulans GBI-30, 6086 supports the immune system at rest and after strenuous exercise. The study product may lessen the window of immune system suppression post-exercise, and enhance stress recovery supporting immunity.","PeriodicalId":16865,"journal":{"name":"Journal of Probiotics & Health","volume":"7 1","pages":"1-7"},"PeriodicalIF":0.0,"publicationDate":"2017-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76456748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-11-30DOI: 10.4172/2329-8901.1000187
S. Shonyela, Guan Wang, Wen-Tao Yang, Guilian Yang, Chunfeng Wang
Lactobacillus species are non-spore-forming, Gram-positive bacteria and lactic acid producing bacteria (LAPB) that naturally inhabits the human and animal gastrointestinal and mouth organs. The aim of this review was to evaluate the new progress regarding the use of Lactobacillus species as live delivery vectors, prevention and treatment of pathogenic and metabolic diseases. Lactobacillus strains as probiotics have been extensively studied and have confirmed that they can absolutely improve performance as live delivery vectors, a treatment option of various diseases such as: hemorrhagic cecal coccidiosis in young poultry, Hypertension, avian flu, obesity, diabetes, Derzsy's disease or parvovirus infection, human immunodeficiency virus infections, irritable bowel syndrome, gastrointestinal disorders, Fungal infections, vaginal eubiosis, fish and shellfish species diseases. We give you an idea about that Lactobacillus species have been proficient in preventing and treating both disorders in animal models and some used for clinical trials. We present the most current studies on the use of Lactobacillus strains that had an impact on an effective immune response to a specific antigen because a variety of antigens have been expressed. Therefore Lactobacillus strains can be considered as good candidates because of its potential for diseases treatment and vaccine development as heterologous protein secretion to date.
{"title":"New Progress Regarding the Use of Lactic Acid Bacteria as Live Delivery Vectors, Treatment of Diseases and Induction of Immune Responses in Different Host Species Focusing on Lactobacillus Species","authors":"S. Shonyela, Guan Wang, Wen-Tao Yang, Guilian Yang, Chunfeng Wang","doi":"10.4172/2329-8901.1000187","DOIUrl":"https://doi.org/10.4172/2329-8901.1000187","url":null,"abstract":"Lactobacillus species are non-spore-forming, Gram-positive bacteria and lactic acid producing bacteria (LAPB) that naturally inhabits the human and animal gastrointestinal and mouth organs. The aim of this review was to evaluate the new progress regarding the use of Lactobacillus species as live delivery vectors, prevention and treatment of pathogenic and metabolic diseases. Lactobacillus strains as probiotics have been extensively studied and have confirmed that they can absolutely improve performance as live delivery vectors, a treatment option of various diseases such as: hemorrhagic cecal coccidiosis in young poultry, Hypertension, avian flu, obesity, diabetes, Derzsy's disease or parvovirus infection, human immunodeficiency virus infections, irritable bowel syndrome, gastrointestinal disorders, Fungal infections, vaginal eubiosis, fish and shellfish species diseases. \u0000We give you an idea about that Lactobacillus species have been proficient in preventing and treating both disorders in animal models and some used for clinical trials. We present the most current studies on the use of Lactobacillus strains that had an impact on an effective immune response to a specific antigen because a variety of antigens have been expressed. Therefore Lactobacillus strains can be considered as good candidates because of its potential for diseases treatment and vaccine development as heterologous protein secretion to date.","PeriodicalId":16865,"journal":{"name":"Journal of Probiotics & Health","volume":"23 1","pages":"1-12"},"PeriodicalIF":0.0,"publicationDate":"2017-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73533395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-11-03DOI: 10.4172/2329-8901.1000188
A. Ahola, V. Harjutsalo, C. Forsblom, R. Freese, S. Mäkimattila, P. Groop
In type 1 diabetes, various metabolic disturbances are frequently observed. Importantly, these may negatively affect individuals’ long-term health outcomes. The use of probiotics has, in other populations, been beneficially associated with many of these risk factors. We, therefore, assessed the cross-sectional associations between the use of probiotics-containing food products or supplements and various health markers in a large population of individuals with type 1 diabetes. Included were 1039 individuals (mean age 46 ± 14 years, 45% men) with type 1 diabetes and without end-stage renal disease. Based on the entries in the diet questionnaire and the food record, participants were divided into those using (Probiotics+) and not using (Probiotics–) probiotics-containing products. Various standard health markers, such as weight, waist circumference, blood pressure, blood lipids, and HbA1c, were measured during the study visit. In all, 403 (39%) individuals reported using probiotics. Adjusted with potential confounders, the rate of overweight/ obesity, body mass index, and waist-to-hip ratio were higher in the Probiotics– group. Moreover, the odds of metabolic syndrome, and its waist, blood pressure, HDL cholesterol, and triglyceride components were higher amongst those not using probiotics. In the normal-weight individuals, using probiotics was associated with significantly better glycaemic control. Using probiotics-containing food products or supplements may beneficially affect many of the traditional risk factors related to the diabetic complications. Randomized controlled trials are required to verify these observations.
{"title":"The Self-reported Use of Probiotics is Associated with Better Glycaemic Control and Lower Odds of Metabolic Syndrome and its Components in Type 1 Diabetes","authors":"A. Ahola, V. Harjutsalo, C. Forsblom, R. Freese, S. Mäkimattila, P. Groop","doi":"10.4172/2329-8901.1000188","DOIUrl":"https://doi.org/10.4172/2329-8901.1000188","url":null,"abstract":"In type 1 diabetes, various metabolic disturbances are frequently observed. Importantly, these may negatively affect individuals’ long-term health outcomes. The use of probiotics has, in other populations, been beneficially associated with many of these risk factors. We, therefore, assessed the cross-sectional associations between the use of probiotics-containing food products or supplements and various health markers in a large population of individuals with type 1 diabetes. \u0000Included were 1039 individuals (mean age 46 ± 14 years, 45% men) with type 1 diabetes and without end-stage renal disease. Based on the entries in the diet questionnaire and the food record, participants were divided into those using (Probiotics+) and not using (Probiotics–) probiotics-containing products. Various standard health markers, such as weight, waist circumference, blood pressure, blood lipids, and HbA1c, were measured during the study visit. \u0000In all, 403 (39%) individuals reported using probiotics. Adjusted with potential confounders, the rate of overweight/ obesity, body mass index, and waist-to-hip ratio were higher in the Probiotics– group. Moreover, the odds of metabolic syndrome, and its waist, blood pressure, HDL cholesterol, and triglyceride components were higher amongst those not using probiotics. In the normal-weight individuals, using probiotics was associated with significantly better glycaemic control. \u0000Using probiotics-containing food products or supplements may beneficially affect many of the traditional risk factors related to the diabetic complications. Randomized controlled trials are required to verify these observations.","PeriodicalId":16865,"journal":{"name":"Journal of Probiotics & Health","volume":"43 1","pages":"1-9"},"PeriodicalIF":0.0,"publicationDate":"2017-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84056711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-10-23DOI: 10.4172/2329-8901-C1-022
David Kyle
{"title":"From dysbiosis to recovery in the infant gut microbiome: A new paradigm in infant nutrition","authors":"David Kyle","doi":"10.4172/2329-8901-C1-022","DOIUrl":"https://doi.org/10.4172/2329-8901-C1-022","url":null,"abstract":"","PeriodicalId":16865,"journal":{"name":"Journal of Probiotics & Health","volume":"58 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83823641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-09-26DOI: 10.4172/2329-8901.1000184
Yang Yang, Han Song, Li Wang, Wei Dong, Zhou Yang, Peng Yuan, Kai Wang, Zhenhui Song
The Transmissible Gastroenteritis Virus (TGEV) causes severe diarrhea and other symptoms which end up in death in young piglets. We have studied the protective effects of the probiotic Lactobacillus plantarum strain N4(Lp) which metabolic products were added to the swine testis (ST) cells with three different orders using MTT cell proliferation assay and CPE analysis. Metabolic products led to dose-dependent rescue of viability of infected cells in a certain order: pre-treatment, post-infection, co-incubation. Pre-treatment of cells with probiotic metabolic products reduced viral proliferation up to 78% at non-cytotoxic concentration 1/4 dilution. The viral yields in pretreatment groups were reduced by over three log10 units. Quantitative real-time PCR revealed the optimal inhibition of TGEV RNA replication was 24 h apply pretreatment way that up to 71% of N gene. Analysised the composition of metabolic products by GC-MS revealed the major component is sugars. Then Exopolysaccharides (EPS) was extracted and showed inhibition effect that co-incubation with TGEV.
{"title":"Antiviral Effects of a Probiotic Metabolic Products against Transmissible Gastroenteritis Coronavirus","authors":"Yang Yang, Han Song, Li Wang, Wei Dong, Zhou Yang, Peng Yuan, Kai Wang, Zhenhui Song","doi":"10.4172/2329-8901.1000184","DOIUrl":"https://doi.org/10.4172/2329-8901.1000184","url":null,"abstract":"The Transmissible Gastroenteritis Virus (TGEV) causes severe diarrhea and other symptoms which end up in death in young piglets. We have studied the protective effects of the probiotic Lactobacillus plantarum strain N4(Lp) which metabolic products were added to the swine testis (ST) cells with three different orders using MTT cell proliferation assay and CPE analysis. Metabolic products led to dose-dependent rescue of viability of infected cells in a certain order: pre-treatment, post-infection, co-incubation. Pre-treatment of cells with probiotic metabolic products reduced viral proliferation up to 78% at non-cytotoxic concentration 1/4 dilution. The viral yields in pretreatment groups were reduced by over three log10 units. Quantitative real-time PCR revealed the optimal inhibition of TGEV RNA replication was 24 h apply pretreatment way that up to 71% of N gene. Analysised the composition of metabolic products by GC-MS revealed the major component is sugars. Then Exopolysaccharides (EPS) was extracted and showed inhibition effect that co-incubation with TGEV.","PeriodicalId":16865,"journal":{"name":"Journal of Probiotics & Health","volume":"5 1","pages":"1-6"},"PeriodicalIF":0.0,"publicationDate":"2017-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81900989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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