Journal of Radiation Research and Applied Sciences最新文献

{"title":"Concentration dependent gamma degradation of inulin in aqueous phase and its impact on antioxidant capacity and probiotic fermentability","authors":"Sudesh ,&nbsp;Sahayog N. Jamdar","doi":"10.1016/j.jrras.2025.102131","DOIUrl":"10.1016/j.jrras.2025.102131","url":null,"abstract":"<div><div>Inulin is a water soluble, non-digestible polysaccharide with diverse food and clinical applications depending on its degree of polymerization. This study investigated the effect of inulin concentration on its gamma-induced depolymerization in aqueous phase by analysing changes in relative viscosity (<em>η</em>_<em>r</em>), solubility (%), total reducing sugar (TRS), free fructose (<em>F</em>_<em>f</em>) content, and •OH radical scavenging activity. The extent of inulin degradation in aqueous solution was influenced by both irradiation dose and sample concentration. Degradation increased with irradiation dose, while concentration and degree of degradation were in inverse relationship. The level of degradation as a result of molecular fragmentation was confirmed by thin layer chromatography (TLC) and gel permeation chromatography (GPC). FT-IR spectral characterization of unirradiated and irradiated samples revealed no significant changes in peak profiles. Furthermore, concentration-dependent degradation was evaluated for its impact on antioxidant capacity and probiotic fermentability. Inulin irradiated at lower concentrations exhibited significantly higher (<em>p &lt; 0.05</em>) ABTS and DPPH radical scavenging activity and probiotic fermentability than samples irradiated at higher concentrations; specifically, at 25 kGy, inulin irradiated in a 5 % (w/v) aqueous system showed ∼3.5-fold higher antioxidant activity and a ∼2.5-log increase in probiotic fermentability compared to the 60 % (w/v) system.</div></div>","PeriodicalId":16920,"journal":{"name":"Journal of Radiation Research and Applied Sciences","volume":"19 1","pages":"Article 102131"},"PeriodicalIF":2.5,"publicationDate":"2025-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145787241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Engineering software-based evaluation of pelvic floor muscle defects in anterior vaginal wall prolapse","authors":"Lifan Shen ,&nbsp;Huijun Bai","doi":"10.1016/j.jrras.2025.102119","DOIUrl":"10.1016/j.jrras.2025.102119","url":null,"abstract":"<div><h3>Objective</h3><div>To assess the relationship between pelvic floor muscle morphology and anterior vaginal wall prolapse using MRI 3D reconstruction and engineering software.</div></div><div><h3>Methods</h3><div>This study conducted a retrospective analysis involving 68 patients diagnosed with anterior vaginal wall prolapse. Magnetic resonance images (MRI) of the relevant pelvic floor muscles from these patients were utilized for three-dimensional reconstruction using Mimics. Subsequently, the IGS models generated by Geomagic Wrap were employed for material property assignment and analysis within SolidWorks. The muscle models were then transformed into visual simulation entities, facilitating the derivation of metrics such as, volume, and surface area of the muscles associated with anterior vaginal wall prolapse. This methodology allowed for a direct assessment of muscle tissue defects and symmetry.</div></div><div><h3>Results</h3><div>In patients with anterior vaginal wall prolapse, statistically significant differences (P &lt; 0.05) were observed in the volume, and maximum thickness of the bulbocavernosus muscle relative to the POP-Q grading of anterior vaginal wall prolapse. Whether there was damage to the obturator internus, ischiocavernosus, levator ani muscle, external urethral sphincter, and external anal sphincter muscles was statistically significant in relation to anterior vaginal wall prolapse (P &lt; 0.05). Symmetry of the ischiocavernosus and levator ani muscle also showed statistical significance concerning anterior vaginal wall prolapse (P &lt; 0.05). Factors related to the level of anterior vaginal wall prolapse included the maximum thickness of the bulbocavernosus muscle (OR = 0.005719), damage to the ischiocavernosus muscle (OR = 0.084754), damage to the levator ani muscle (OR = 0.083995), damage to the external urethral sphincter (OR = 0.005917), and damage to the external anal sphincter (OR = 0.004091) (P &lt; 0.05).</div></div><div><h3>Conclusion</h3><div>Utilizing engineering software and 3D reconstruction to create muscle models offers an impartial and three-dimensional depiction of the morphological alterations in pelvic floor muscles, along with their connection to anterior vaginal wall prolapse. This can help predict and manage the potential impacts on patients.</div></div>","PeriodicalId":16920,"journal":{"name":"Journal of Radiation Research and Applied Sciences","volume":"19 1","pages":"Article 102119"},"PeriodicalIF":2.5,"publicationDate":"2025-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145787189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CT and MRI measurement of the femoral anterior tangent for the rotational reference axis of femoral prostheses","authors":"Haibo Liang,&nbsp;Haiquan Deng,&nbsp;Jianchao Sun,&nbsp;Kaitian Wu,&nbsp;Shuzhen Li","doi":"10.1016/j.jrras.2025.102130","DOIUrl":"10.1016/j.jrras.2025.102130","url":null,"abstract":"<div><h3>Background</h3><div>This study aims to evaluate the value of the femoral anterior tangent (FAT) as a reference axis for the rotation of femoral prostheses on computed tomography (CT) and magnetic resonance imaging (MRI).</div></div><div><h3>Methods</h3><div>We retrospectively included 66 patients (83 knees) with primary knee osteoarthritis. FAT, clinical transepicondylar axis (cTEA), surgical transepicondylar axis (sTEA), anterior-posterior axis (APA), perpendicular axis to the posterior-anterior axis of the femoral condyles (pAPA), and posterior condylar axis (PCA) were measured on CT and MRI. The rotation angles of FAT, pAPA, and PCA relative to cTEA and sTEA were measured on the same layer. Patients were further subgrouped based on Kellgren-Lawrence (K-L) grade and gender to assess the variance of different rotational reference axes.</div></div><div><h3>Results</h3><div>The mean values of FAT/cTEA, FAT/sTEA, and pAPA/cTEA measured on CT were significantly lower, indicating greater internal rotation, than those measured on MRI (P &lt; 0.05). There were no statistical differences between FAT/sTEA, FAT/cTEA, pAPA/cTEA, and PCA/cTEA among genders (P &gt; 0.05). The CT measurement of pAPA/cTEA in K-L grade 3 knees was −1.1° ± 3.7°, significantly higher than in K-L grade 4 knees (P = 0.036). FAT/cTEA, FAT/sTEA, and PCA/cTEA demonstrated higher stability in various subgroups of knee osteoarthritis.</div></div><div><h3>Conclusion</h3><div>The rotational angles of femoral prostheses measured by CT for FAT and other reference axes show increased internal rotation compared to MRI measurements. Both measurement methods provide relatively stable values for FAT measurements. Clinicians should be cautious of the numerical differences between MRI and CT measurements in clinical applications and should consider modality-specificreference values for pre-operative planning.</div></div>","PeriodicalId":16920,"journal":{"name":"Journal of Radiation Research and Applied Sciences","volume":"19 1","pages":"Article 102130"},"PeriodicalIF":2.5,"publicationDate":"2025-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145837399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Radiological safety assessment of packaged bottle drinking water in India – Age dependent analysis","authors":"Mahasin Gazi ,&nbsp;Arindam Kumar Naskar ,&nbsp;Argha Deb","doi":"10.1016/j.jrras.2025.102128","DOIUrl":"10.1016/j.jrras.2025.102128","url":null,"abstract":"<div><div>Radioactive radon (²²²Rn) concentrations in 121 different brands of packaged bottle drinking water (PBDW) samples from different states of India have been assessed in the present study using a potable AlphaGUARD radon monitor along with AlphaGUARD aqua kit. Among the collected samples, the production sites of 72 number of PBDW samples are different places of West Bengal and the remaining 49 samples are different places of other different states of India. Thus, assessing the radon levels in different brands of packaged drinking water that are accessed from most parts of India gives a general picture of the ²²²Rn concentrations in packaged bottled drinking water throughout India.</div><div>Measured radon concentration levels vary between 0.19 and 5.81 Bq/l with an average value of 1.36 Bq/l. Radon concentration of no sample has exceeded the WHO, IAEA and EU Commission prescribed reference level of 100 Bq/l or even USEPA's proposed MCL (11.11 Bq/l).</div><div>The measured radon concentrations of the collected samples manifest no significant correlation with either Total Dissolved Solids (TDS) or pH values of the samples.</div><div>Annual effective dose (AED) due to radon from these bottled drinking water for three age groups namely infants, children and adults are found to vary between 0.66 and 20.00 μSv/y with a mean of 4.68 ± 0.29 μSv/y, 0.82–25.00 μSv/y with a mean of 5.84 ± 0.36 μSv/y and 0.73–22.30 μSv/y with a mean of 5.21 ± 0.32 μSv/y respectively. Assessment of dose shows that total annual effective dose to the children is the highest among the age groups. No sample has exceeded the WHO, ICRP and the EU commission proposed reference dose limit of 100 μSv/y.</div></div>","PeriodicalId":16920,"journal":{"name":"Journal of Radiation Research and Applied Sciences","volume":"19 1","pages":"Article 102128"},"PeriodicalIF":2.5,"publicationDate":"2025-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145787138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Medical and radiation science analysis using a probability U-Net with a GRU model","authors":"Ahmed I. Taloba,&nbsp;Rayan Alanazi","doi":"10.1016/j.jrras.2025.102134","DOIUrl":"10.1016/j.jrras.2025.102134","url":null,"abstract":"<div><div>Accurate and interpretable segmentation of CT images of the lungs is critical for early diagnosis and treatment planning in pulmonary diseases. The current deep learning (DL) models tend to lack uncertainty quantification, lack consistency over time, and do not extrapolate into ambiguous areas. To solve these problems, this study suggests a hybrid DL model that combines U-Net with Gated Recurrent Units (GRUs) and a probabilistic latent space to segment based on uncertainty. The encoder learns both spatial and temporal features using GRU-enhanced convolutional blocks, and the probabilistic module of the bottleneck addresses aleatory and epistemic uncertainty via Monte Carlo sampling. This joint temporal modeling and probabilistic representation allows the system to perform temporally consistent segmentation with only one lightweight architecture. The experiments on the IQ-OTH/NCCD lung CT dataset (1295 annotated images) demonstrate that the proposed model achieves 98.9 % accuracy and a 91.5 % Dice coefficient, which is 7–15 % higher than state-of-the-art models, including ResNet18 with MC Dropout and SkinSAM. The model also achieves an Expected Calibration Error of just 2.8 % which justifies its validity in the clinic. Uncertainty maps can also aid decision-making regarding tumor boundaries. Compared with previous applications such as Probabilistic U-Net and GRU-Net, which operate independently, the suggested U-Net-GRU-Probabilistic framework offers an integrated, confident, and understandable method for segmenting lung CT. Demonstrates potential for real-time diagnostic deployment.</div></div>","PeriodicalId":16920,"journal":{"name":"Journal of Radiation Research and Applied Sciences","volume":"19 1","pages":"Article 102134"},"PeriodicalIF":2.5,"publicationDate":"2025-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145787137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ARHGAP11A promotes proliferation and invasion through RhoA/RAC1 in LUAD","authors":"Xinyu Liu ,&nbsp;Xuesong Zhao ,&nbsp;Haifeng Hu ,&nbsp;Tianyu Zhang ,&nbsp;Yi Qi ,&nbsp;Bo Liu ,&nbsp;Bing Lan ,&nbsp;Bin Li","doi":"10.1016/j.jrras.2025.102123","DOIUrl":"10.1016/j.jrras.2025.102123","url":null,"abstract":"<div><h3>Background</h3><div>Lung adenocarcinoma (LUAD) ranks among the most common subtypes of lung cancer, characterized by a poor prognosis due to the scarcity of effective treatment options. Functional role and underlying mechanisms of Rho GTPase-activating protein 11A (ARHGAP11A) in LUAD remain insufficiently characterized. The study aims to explore it, with the hypothesis that ARHGAP11A is a pivotal driver of tumor progression and a potential therapeutic target.</div></div><div><h3>Methods</h3><div>The research employed bioinformatics analysis to identify ARHGAP11A's overexpression in LUAD and its correlation with poor prognosis. To evaluate how reducing ARHGAP11A affects LUAD cell movement and invasion, various functional tests such as cell scratch and transwell cell invasion assays were conducted. Additionally, the study investigated ARHGAP11A's involvement in LUAD epithelial-mesenchymal transition and its regulation through the RhoA/RAC1 pathway using RAC1-specific inhibitor EHT-1864. In vivo tumor growth was assessed following ARHGAP11A knockdown, and the efficacy of the RAC1-specific inhibitor EHT-1864 in reducing tumor growth was tested.</div></div><div><h3>Results</h3><div>The bioinformatics investigation indicated that ARHGAP11A is upregulated in LUAD and correlates with unfavorable outcomes. Cell migration and invasion were inhibited after ARHGAP11A knockdown. The study also implicated ARHGAP11A in LUAD epithelial-mesenchymal transition and demonstrated its regulation through the RhoA/RAC1 pathway using a RAC1-specific inhibitor. In vivo tumor growth was significantly inhibited by ARHGAP11A knockdown, and treatment with EHT-1864 reduced tumor growth, indicating a potential therapeutic strategy.</div></div><div><h3>Conclusion</h3><div>The study's findings establish ARHGAP11A as a key factor in LUAD progression, enhancing cell proliferation and invasion. These results warrant further exploration of ARHGAP11A's mechanisms and clinical applications, potentially leading to new therapeutic strategies for LUAD treatment.</div></div>","PeriodicalId":16920,"journal":{"name":"Journal of Radiation Research and Applied Sciences","volume":"19 1","pages":"Article 102123"},"PeriodicalIF":2.5,"publicationDate":"2025-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145787132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Predicting cognitive decline in Parkinson's disease using multimodal MRI features and serum inflammatory markers","authors":"Ren Dong ,&nbsp;Haiqing Shen ,&nbsp;Qianning Li","doi":"10.1016/j.jrras.2025.102124","DOIUrl":"10.1016/j.jrras.2025.102124","url":null,"abstract":"<div><h3>Purpose</h3><div>Cognitive decline is a significant concern in Parkinson's disease, affecting patient quality of life and increasing healthcare burden. This study aims to construct and validate a risk prediction model for cognitive decline in Parkinson's disease patients using multimodal MRI features and serum inflammatory markers.</div></div><div><h3>Methods</h3><div>A retrospective case-control study was conducted involving 413 Parkinson's disease patients with normal cognition at baseline. These patients were divided into a primary cohort (n = 321) and an external validation cohort (n = 92). Patients were followed for 1–4 years, and patients with disease progression were compared with those without progression. Multimodal MRI scans and serum inflammatory marker assessments were performed. Univariate, multivariate logistic regression, and receiver operator characteristic (ROC) analyses were used to identify predictors of cognitive decline.</div></div><div><h3>Results</h3><div>Compared with the non-progression group, the progression group showed significant differences in T1 characteristics, including cortical thickness of the left hemisphere (LH) postcentral gyrus (P = 0.008), left hippocampal volume (P = 0.005), and right hippocampal volume (P = 0.004). The progression group showed significant differences in Susceptibility-Weighted Imaging (SWI) characteristics. Symptoms on the opposite side of Substantia Nigra pars compacta (SNc) were more pronounced in the non-progression group compared to the progression group (P = 0.006). Similarly, for symptoms on the same side of SNc, the non-progression group showed more prominent findings than the progression group (P &lt; 0.001). Serum inflammatory marker analysis indicated that the progression group also showed significant differences in lymphocyte count (P = 0.023), neutrophil-to-lymphocyte ratio (NLR) (P = 0.002), lymphocyte-to-monocyte ratio (LMR) (P = 0.003), and albumin-to-fibrinogen ratio (AFR) (P = 0.004). The combined prediction model demonstrated high diagnostic accuracy, with an area under the curve (AUC) of 0.809 in the primary cohort and 0.812 in the external validation cohort.</div></div><div><h3>Conclusion</h3><div>The developed model combining multimodal MRI features and serum inflammatory markers effectively identifies key factors influencing cognitive decline in Parkinson's disease, offering high diagnostic accuracy and valuable clinical insights for early detection and personalized treatment.</div></div>","PeriodicalId":16920,"journal":{"name":"Journal of Radiation Research and Applied Sciences","volume":"19 1","pages":"Article 102124"},"PeriodicalIF":2.5,"publicationDate":"2025-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145787194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SF3B3-mediated alternative splicing of ATP2B4 via exon 21 skipping contributes to the progression of bladder cancer","authors":"Cong Cheng ,&nbsp;Tao Yang ,&nbsp;Li Wang,&nbsp;Youji Yan,&nbsp;Yixiang Liao","doi":"10.1016/j.jrras.2025.102121","DOIUrl":"10.1016/j.jrras.2025.102121","url":null,"abstract":"<div><h3>Background</h3><div>While alternative splicing is increasingly recognized in cancer, its specific regulators and functional consequences in bladder cancer remain largely uncharted. This study aims to investigate the role of abnormal alternative splicing of ATP2B4 in the progression of bladder cancer.</div></div><div><h3>Methods</h3><div>Exon sequencing data from The Cancer Genome Atlas were analyzed using bioinformatics algorithms, revealing significant exon skipping events in bladder cancer. Then, confirm the dysregulated expression of specific transcripts in bladder cancer tissues. Additionally, ATP2B4 was overexpressed or knocked down in tumor cells for in vitro functional experiments.</div></div><div><h3>Results</h3><div>Experimental results show that ATP2B4 produces two transcripts: the full-length transcript (ATP2B4-V1) and the spliced transcript (ATP2B4-V2), where the spliced transcript skips exon 21. ATP2B4-v2 is highly expressed in bladder cancer tissues, while ATP2B4-v1 is mainly expressed in non-cancerous tissues and normal bladder epithelium. Moreover, a higher PSI (Percent Spliced In) value for exon 21, indicative of greater ATP2B4-V1 expression, was correlated with improved survival in bladder cancer patients. In vitro functional assays demonstrated that the ATP2B4 splicing variant (ATP2B4-V2) enhanced the proliferation, migration, and invasion of bladder cancer cells, whereas the full-length transcript (ATP2B4-V1) exerted an opposing effect. We also identified a group of splicing factors, among which the splicing factor SF3B3 (Splicing Factor 3b Subunit 3) was found to promote the skipping of exon 21 of ATP2B4, and explored their role in regulating ATP2B4 precursor mRNA. The results showed that SF3B3 could promote the skipping of exon 21 of ATP2B4, resulting in an increased proportion of bladder cancer cells and enhanced carcinogenicity.</div></div><div><h3>Conclusion</h3><div>SF3B3 promotes the skipping of ATP2B4 exon 21, leading to the preferential expression of an oncogenic isoform (ATP2B4-V2) which promotes the malignant phenotype of bladder cancer cells, suggesting that targeting alternative splicing regulation may represent a promising therapeutic strategy.</div></div>","PeriodicalId":16920,"journal":{"name":"Journal of Radiation Research and Applied Sciences","volume":"19 1","pages":"Article 102121"},"PeriodicalIF":2.5,"publicationDate":"2025-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145787195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effects of acute and chronic exposure of 2100 MHz radiofrequency radiation on rat mismatch negativity","authors":"Hakan Er ,&nbsp;Enis Hidisoglu ,&nbsp;Deniz Kantar ,&nbsp;Alev Duygu Acun ,&nbsp;Gokhan Akkoyunlu ,&nbsp;Sukru Ozen ,&nbsp;Piraye Yargicoglu","doi":"10.1016/j.jrras.2025.102126","DOIUrl":"10.1016/j.jrras.2025.102126","url":null,"abstract":"<div><div>Cell phones operate by emitting radiofrequency radiation (RFR), a form of electromagnetic radiation (EMR). Consequently, ongoing researches target to determine whether it poses potential risks to human health. One of these risks is related with brain and auditory system. This study aims to examine the impact of acute and chronic exposure to 2100 MHz radiofrequency radiation on mismatch negativity (MMN) in rats.</div><div>In this study, we established 1-week (RFR1) and 10-week (RFR10) RFR groups from rats, which were subjected to 2100 MHz RFR exposure. Cage control groups (CC1, CC10) and sham groups (S1, S10) that were not subjected to RFR for equivalent durations were also established. Following auditory event-related potential (AERP) recordings, MMN waves were computed and analyzed. Additionally, brain samples were collected and biochemical and histological analyses were performed.</div><div>The RFR1 group exhibited a reduction in AMPAR GluR2 subunit protein levels relative to the CC1 and S1 groups, although GFAP protein levels increased. Conversely, the opposite was observed in the chronic groups. Edema of astrocytic endfeet, mitochondrial damage, and lysosomal vesicles were identified in the RFR1 group. The MMN amplitude was found to be reduced in the RFR1 group relative to the CC1 group. The RFR1 group exhibited a reduction in delta and theta power relative to the S1/CC1 groups. Alpha coherence diminished in the RFR1 group relative to the S1 group, however it augmented in the RFR10 group compared to the S10 group.</div><div>The assessment of event-related potentials indicated that 2100 MHz RFR led to a decrease in MMN amplitude, power spectrum, and coherence values in the RFR1 group relative to the S1 and CC1 groups, but an increase was observed in the RFR10 group compared to the S10 group. Consequently, in the acute period, 2100 MHz RFR may have adverse effects on auditory sensory memory.</div></div>","PeriodicalId":16920,"journal":{"name":"Journal of Radiation Research and Applied Sciences","volume":"19 1","pages":"Article 102126"},"PeriodicalIF":2.5,"publicationDate":"2025-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145787133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isocitrate dehydrogenase 2 promotes tumor progression and glycolysis in cervical squamous cell carcinoma by activating the HIF1A signaling pathway","authors":"Xiaojuan Liu ,&nbsp;Lisha Shu ,&nbsp;Huiying Zhang","doi":"10.1016/j.jrras.2025.102113","DOIUrl":"10.1016/j.jrras.2025.102113","url":null,"abstract":"<div><h3>Background</h3><div>Isocitrate dehydrogenase 2 (IDH2), a mitochondrial enzyme central to metabolic processes, has been associated with tumorigenesis in multiple cancers; however, its functional significance in cervical squamous cell carcinoma (CSCC) remains unclear.</div></div><div><h3>Methods</h3><div>We assessed IDH2 expression in clinical CSCC samples and established cell lines. Functional analyses, including IDH2 knockdown in CaSki cells, were performed to evaluate its impact on cellular proliferation, migratory capacity, and glycolytic metabolism. To uncover the molecular mechanism, we investigated the interplay between IDH2 and hypoxia-inducible factor 1-alpha (HIF1α), along with the expression of downstream glycolytic targets such as hexokinase 2 (HK2) and lactate dehydrogenase A (LDHA). The in vivo role of IDH2 was further examined using xenograft models in nude mice, monitoring tumor growth and the levels of HIF1α, Ki67, and glycolytic indicators.</div></div><div><h3>Results</h3><div>IDH2 was markedly overexpressed in both CSCC tissues and cell lines, with elevated levels correlating with with elevated levels associated with enhanced malignant potential in functional assays. Depletion of IDH2 inhibited cell proliferation, migration, and glycolytic activity in CaSki cells. Mechanistically, IDH2 was shown to upregulate HIF1α, thereby enhancing the transcription of glycolytic enzymes HK2 and LDHA. Notably, the oncogenic and metabolic effects induced by IDH2 were reversed upon HIF1α silencing, confirming its essential mediating role. In mouse models, IDH2 knockdown significantly suppressed tumor growth and downregulated HIF1α, Ki67, and key glycolytic markers. Notably, the oncogenic and metabolic effects induced by IDH2 were reversed upon HIF1α silencing, confirming its essential mediating role.</div></div><div><h3>Conclusion</h3><div>Our study identifies an IDH2-HIF1α-glycolysis signaling cascade that promotes CSCC progression. These findings establish IDH2 as a key driver of metabolic reprogramming in cervical cancer and support its potential as a target for therapeutic strategies, potentially involving the repurposing of existing IDH2 inhibitors, aimed at disrupting cancer metabolism in CSCC.</div></div>","PeriodicalId":16920,"journal":{"name":"Journal of Radiation Research and Applied Sciences","volume":"19 1","pages":"Article 102113"},"PeriodicalIF":2.5,"publicationDate":"2025-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145787134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}