Journal of Radiation Research and Applied Sciences最新文献

{"title":"Silver ion alginate dressing for infection control in type Ⅱ diabetic rats wound: an in vitro and in vivo study","authors":"Yao Liang ,&nbsp;Shiyuan Xie ,&nbsp;Zhongwen Yu ,&nbsp;Bin Yang ,&nbsp;Jinbin Wei","doi":"10.1016/j.jrras.2025.102156","DOIUrl":"10.1016/j.jrras.2025.102156","url":null,"abstract":"<div><h3>Background</h3><div>Diabetic wounds present a formidable therapeutic challenge due to their persistent nature and high complication rates. In this study, the new silver alginate dressing was evaluated to determine whether it improves wound healing in type Ⅱ diabetic rats by comparison with the commercially available silver alginate dressing Biatain® in vivo and in vitro. This study demonstrates that the novel dressing may provide a viable alternative approach to the current management of diabetic wounds.</div></div><div><h3>Methods</h3><div>The in vitro part involved the evaluation of its water vapor transmission ability, water-uptake ability, and antibacterial ability. Then, Goto-Kakizaki diabetic rats were selected for in vivo experiments to establish the diabetic infection wound model. The healing rate of wounds and the bacterial count on wounds and wound dressings were studied. Detection of fibrinogen (FIB), procalcitonin (PCT), and high-sensitivity C-reactive protein (hs-CRP) by ELISA, Platelet endothelial cell adhesion molecule-1 (CD31) and Alpha-smooth muscle actin (α-SMA) protein expression by histological staining and determination of silver by microwave digestion-inductively coupled plasma mass spectrometry.</div></div><div><h3>Results</h3><div>In comparison with the commercially available Biatain®, The silver alginate ion dressing has about twice the water absorption capacity of Biatain® (P &lt; 0.01) and On day 14, the wound healing rate in the sample group demonstrated a significant increase of 15.6 % relative to the model group, with wounds approaching near-complete closure.</div></div><div><h3>Conclusion</h3><div>The newly developed silver-ion alginate dressing enhanced wound closure, reduced infection and healing time, and lowered dressing change frequency, demonstrating comparable efficacy to the commercial reference product (Biatain®) as a practical alternative for diabetic wound care.</div></div>","PeriodicalId":16920,"journal":{"name":"Journal of Radiation Research and Applied Sciences","volume":"19 1","pages":"Article 102156"},"PeriodicalIF":2.5,"publicationDate":"2026-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145925209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A predictive model for functional outcomes in patients with acute anterior circulation stroke based on DWI-ASPECTS and serum homocysteine","authors":"Meilin Wang ,&nbsp;Jinsong Cheng","doi":"10.1016/j.jrras.2026.102163","DOIUrl":"10.1016/j.jrras.2026.102163","url":null,"abstract":"<div><h3>Background</h3><div>Precise forecasting of functional outcomes is crucial for managing acute anterior circulation stroke (AACS). We sought to develop and validate a predictive model for functional outcomes by integrating the DWI-ASPECTS score and serum homocysteine levels.</div></div><div><h3>Methods</h3><div>We retrospectively examined information from consecutive AACS patients admitted between January 2022 and January 2025. The individuals were classified into favorable (modified Rankin Scale [mRS] ≤ 2) and unfavorable (mRS &gt;2) outcomes groups depending on their 90-day mRS score. Upon admission, clinical parameters, including the National Institutes of Health Stroke Scale score, were assessed. Diffusion-weighted imaging was performed within 24 h to calculate the DWI-ASPECTS. Blood samples taken from a vein after an overnight fast were gathered within a 24-h timeframe for laboratory testing, including serum homocysteine, glycated hemoglobin, and lipid profiles.</div></div><div><h3>Results</h3><div>We included 122 patients, among whom 68 cases belonged to the favorable and 54 cases belonged to the unfavorable outcome group. Unfavorable outcome patients were significantly older (P = 0.017), exhibited higher baseline NIHSS scores, lower DWI-ASPECTS scores, and demonstrated elevated serum homocysteine levels (all P &lt; 0.001). Multivariate analysis showed that DWI-ASPECTS score (OR = 0.270, P &lt; 0.001) and serum homocysteine level (OR = 1.539, P &lt; 0.001) independently predicted functional outcome. DWI-ASPECTS (AUC = 0.852) and homocysteine (AUC = 0.844) demonstrated high predictive accuracy. The final nomogram model, which combined these two variables, showed excellent discrimination with an AUC of 0.939 and was well-calibrated.</div></div><div><h3>Conclusion</h3><div>The developed nomogram, incorporating DWI-ASPECTS and serum homocysteine, demonstrates potential as an effective and accurate method for forecasting functional outcomes in AACS patients, offering a novel approach that integrates neuroanatomical and systemic metabolic information beyond models relying on single parameters.</div></div>","PeriodicalId":16920,"journal":{"name":"Journal of Radiation Research and Applied Sciences","volume":"19 1","pages":"Article 102163"},"PeriodicalIF":2.5,"publicationDate":"2026-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145925212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dose optimization of stereotactic radiosurgery by 18F-FHBG PET/CT tracking of HSV-TK gene vectors in recurrent glioblastoma: A prospective controlled study","authors":"Jing Ye ,&nbsp;Wenbo Cai ,&nbsp;Fobao Huang ,&nbsp;Xi zhen Xu","doi":"10.1016/j.jrras.2025.102127","DOIUrl":"10.1016/j.jrras.2025.102127","url":null,"abstract":"<div><h3>Objective</h3><div>To test whether ¹⁸F-FHBG PET tracking of HSV-TK gene-vector expression can guide stereotactic radiosurgery (SRS) dose escalation to improve outcomes versus MRI-only SRS.</div></div><div><h3>Methods</h3><div>In a single-centre, open-label, randomized phase II trial, 90 patients with recurrent glioblastoma were assigned (1:1) to PET-guided biological target volume SRS (PET-BTV) or standard single-fraction SRS (S-SRS). Primary endpoint was 6-month progression-free survival (PFS₆); key secondary endpoints included local control, 12-month overall survival (OS₁₂), ≥grade-3 toxicity, neurocognition (Trail-Making Test-B), and PET reproducibility. PET test–retest reliability and inter-observer agreement were assessed. Time-to-event outcomes used Kaplan–Meier/Cox models; toxicity used Firth logistic regression; repeated measures used appropriate ANOVA.</div></div><div><h3>Results</h3><div>PET metrics (SUV_max, SUV_mean, MTV) showed excellent test–retest reliability (intraclass correlation coefficients &gt;0.85) and robust contouring agreement. Versus S-SRS, PET-BTV improved PFS (hazard ratio [HR] ≈ 0.54) and local control (HR ≈ 0.48; both <em>P</em> &lt; 0.05), with no significant differences in OS₁₂ (HR ≈ 0.71) or ≥ grade-3 toxicity (HR ≈ <em>0.43;</em> both <em>P</em> &gt; 0.05). Multivariable Cox confirmed treatment group (HR ≈ 0.55), smaller tumour volume (HR ≈ 1.06 per unit), and higher Karnofsky score (HR ≈ 0.97) as independent predictors of longer PFS. PET response (decrease in SUV_max) correlated inversely with risk of progression. Trail-B favored PET-BTV (significant group effect and interaction), suggesting preserved neurocognition.</div></div><div><h3>Conclusions</h3><div>¹⁸F-FHBG PET-guided intratumoral dose painting is feasible, reproducible, and improves PFS and local control without increasing high-grade toxicity, supporting imaging-driven precision re-irradiation for recurrent glioblastoma.</div></div>","PeriodicalId":16920,"journal":{"name":"Journal of Radiation Research and Applied Sciences","volume":"19 1","pages":"Article 102127"},"PeriodicalIF":2.5,"publicationDate":"2026-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145925210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Coptisine suppresses pulmonary nodule carcinogenesis by inducing CAFs mitochondrial apoptosis via Sirt3/YME1L1","authors":"Jin-Liang Hu ,&nbsp;Kun Lv ,&nbsp;Jie-Zhong Hu ,&nbsp;Zheng Tang ,&nbsp;Pia Vanessa C. Basilio","doi":"10.1016/j.jrras.2025.102148","DOIUrl":"10.1016/j.jrras.2025.102148","url":null,"abstract":"<div><div>Coptisine, an active alkaloid from <em>Coptis chinensis</em>, exhibits potent anticancer effects by targeting cancer-associated fibroblasts (CAFs) in the tumor microenvironment. This study investigates its mechanism in attenuating lung via Sirt3-mediated deacetylation of YME1L1, regulating mitochondrial apoptosis in CAFs. Orthotopic lung cancer models were established in C57BL/6 mice using SCLC-2 cells, treated with Coptisine (60 or 120 mg/kg/day). In vitro, primary pulmonary fibroblasts (CAFs) co-cultured with SCLC-2 cells were assessed for proliferation (CCK-8), metastasis (Transwell), apoptosis (ELISA), mitochondrial potential (JC-1), and ROS (DCFH-DA). Coptisine inhibited tumor growth, improved lung function, and reduced myofibroblast markers (α-SMA, FAP). It upregulated Bax and Sirt3 while downregulating YME1L1, BCL-2, and EMT proteins (e.g., Vimentin), damaging to mitochondrial function. Mechanistically, Coptisine activated Sirt3 to deacetylate YME1L1, inducing mitochondrial fragmentation in CAFs. These findings highlight Coptisine's potential as a lung cancer therapy by targeting the Sirt3/YME1L1 pathway in CAFs.</div></div>","PeriodicalId":16920,"journal":{"name":"Journal of Radiation Research and Applied Sciences","volume":"19 1","pages":"Article 102148"},"PeriodicalIF":2.5,"publicationDate":"2026-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145925213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sound-based navigation system for visually impaired individuals","authors":"Emad Malaekah ,&nbsp;Othman Alfahad ,&nbsp;Mohsen Bakouri ,&nbsp;Adel Gadallah ,&nbsp;Suvad Selman ,&nbsp;Ahmed Al Rashdi ,&nbsp;Husham Saied","doi":"10.1016/j.jrras.2026.102160","DOIUrl":"10.1016/j.jrras.2026.102160","url":null,"abstract":"<div><div>Millions of people worldwide have impaired vision for various reasons, such as cataracts, glaucoma, AMD, diabetic retinopathy, trauma, infections, genetic disorders, and amblyopia. Visual impairment ranges from normal vision to complete blindness, with varying severities that affect daily life. This study presents a sound-based navigation system to improve indoor mobility for visually impaired individuals. The system combines voice recognition, LED-guided floor paths, and real-time haptic feedback to help users follow predefined routes. Users issue voice commands to activate illuminated floor strips, which a robot cart follows using light-dependent resistor (LDR) sensors. An electromechanical compass provides accurate orientation, while a vibrating handle delivers haptic alerts for real-time directional adjustments. Bluetooth Low Energy (BLE) determines the user's location and destination to guide them along the correct route. Experimental results show high accuracy: 91.04 % voice recognition, 4 % navigation error due to sensor noise, and 3 % speed regulation error from electrical fluctuations. The proposed solution is cost-effective and user-friendly, promoting greater independence and safety for visually impaired individuals.</div></div>","PeriodicalId":16920,"journal":{"name":"Journal of Radiation Research and Applied Sciences","volume":"19 1","pages":"Article 102160"},"PeriodicalIF":2.5,"publicationDate":"2026-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145925211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring thermodynamic processes through CFD of free convection of Bingham fluids in innovative hexagonal enclosure (Papanastasiou model)","authors":"Benhanifia Kada ,&nbsp;Mebarki Brahim ,&nbsp;Ahmed Remlaoui ,&nbsp;Keddar Mohammed ,&nbsp;Syed M. Hussain ,&nbsp;Hijaz Ahmad ,&nbsp;Neissrien Alhubieshi ,&nbsp;Assmaa Abd-Elmonem ,&nbsp;Bandar M. Fadhl ,&nbsp;Basim M. Makhdoum","doi":"10.1016/j.jrras.2026.102161","DOIUrl":"10.1016/j.jrras.2026.102161","url":null,"abstract":"<div><div>This research presents a unique enclosure geometry, namely, a hexagonal finned cavity, for the analysis of Bingham–Papanastasiou fluid natural convection, which is examined numerically through COMSOL Multiphysics. The momentum and energy equations that govern the phenomena are solved via the Galerkin approach. The external walls have been assumed to be cold, while the inner wall has been taken as hot. A thorough investigation of the thermo-hydrodynamic flow is carried out considering the nominal value of the Rayleigh number (Ra), the range of plasticity Bn (1–50), and the obstacle lengths L (0.1, 0.4). The findings demonstrate that the rate of heat transfer (<em>Nu</em>) is positively correlated with the buoyancy parameter (Ra) while it is inversely related to the plasticity parameter (Bn), with larger values of the latter resulting in lesser heat transfer. This reduction seems to occur due to lame yield stress at a bigger Bn, damping the fluid flow inside the enclosure. The streamline patterns further confirm this trend, showing a significant weakening of circulation at high plasticity levels, particularly for Bn = 50. The effect of obstacle length can be seen on the streamline and isotherm contours, which may indicate the intermediate fin length (L = 0.2) for Rayleigh Number (Ra) as the best, as it increases vortex creation and supports good circulation in the cavity, thus enhancing the heat transfer performance.</div></div>","PeriodicalId":16920,"journal":{"name":"Journal of Radiation Research and Applied Sciences","volume":"19 1","pages":"Article 102161"},"PeriodicalIF":2.5,"publicationDate":"2026-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145924602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Artificial intelligence in MRI clinical practice: From historical innovation to emerging trends","authors":"Shrooq T. Aldahery","doi":"10.1016/j.jrras.2026.102158","DOIUrl":"10.1016/j.jrras.2026.102158","url":null,"abstract":"","PeriodicalId":16920,"journal":{"name":"Journal of Radiation Research and Applied Sciences","volume":"19 1","pages":"Article 102158"},"PeriodicalIF":2.5,"publicationDate":"2026-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145920842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A machine learning-based gene signature reveals radiotherapy-induced remodeling of the immune microenvironment in esophageal cancer","authors":"Xiaoyu He ,&nbsp;Zhuo Li ,&nbsp;Yao Liu ,&nbsp;Junsheng Chen ,&nbsp;Zihao Yuan ,&nbsp;Lina Huang ,&nbsp;Ting Wang","doi":"10.1016/j.jrras.2025.102129","DOIUrl":"10.1016/j.jrras.2025.102129","url":null,"abstract":"<div><h3>Objective</h3><div>This study aimed to develop a machine learning-based signature using radiotherapy-responsive genes to characterize immune infiltration dynamics and evaluate drug sensitivity in esophageal cancer (EC) before and after radiotherapy.</div></div><div><h3>Methods</h3><div>Based on the GSE137867 dataset from GEO, which includes 4 pre-radiotherapy and 4 post-radiotherapy esophageal cancer samples, we identified significantly differentially expressed genes (DEGs) through differential analysis. Gene Ontology (GO) analysis and Gene Set Enrichment Analysis (GSEA) were performed based on the DEGs. Signature genes were screened using Lasso and SVM machine learning algorithms. These signature genes underwent CIBERSORT immune infiltration analysis, ssGSEA, ESTIMATE immune scoring, drug sensitivity analysis, and ceRNA network analysis. To validate the expression profiles of the target genes, we cultured the esophageal cancer OE19 cell line and performed Reverse Transcription Quantitative Real-Time Polymerase Chain Reaction (RT-qPCR).</div></div><div><h3>Results</h3><div>After identifying 28 DEGs, GO and GSEA analysis revealed that these DEGs were enriched in regulation of memory/regulatory T cell differentiation, chemokine activity and cytokine-cytokine receptor interaction pathways. Through Lasso and SVM, three signature genes were identified: ZDHHC11B, CD46, and PFKFB3. CIBERSORT analysis showed a marked elevation in CD8⁺ T cells after radiotherapy. Drug sensitivity analysis revealed that CD46 was negatively correlated with cisplatin, positively correlated with sapitinib, while PFKFB3 showed a negative correlation with linsitinib. The ceRNA network included 21 miRNAs, 3 mRNAs, and 18 lncRNAs. Specifically, 21 miRNA-mRNA regulatory pairs and 18 lncRNA-mRNA regulatory pairs were identified. RT-qPCR showed that compared with the control group, ZDHHC11B decreased significantly in the post-radiotherapy group, while CD46 and PFKFB3 increased significantly.</div></div><div><h3>Conclusion</h3><div>This study comprehensively investigated the immune landscape of EC pre- and post-radiotherapy and analyzed drug sensitivity of these signature genes, thereby elucidating alterations in the immune microenvironment of EC and nominating ZDHHC11, CD46, and PFKFB3 as potential biomarkers for predicting immune status and therapeutic sensitivity.</div></div>","PeriodicalId":16920,"journal":{"name":"Journal of Radiation Research and Applied Sciences","volume":"19 1","pages":"Article 102129"},"PeriodicalIF":2.5,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145880593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated RP-HPLC degradation profiling and Monte Carlo modeling for evaluating gamma-ray radiosterilization suitability of imidazole antifungals","authors":"Abdelmoneim Saleh ,&nbsp;Zehra Üstün ,&nbsp;Ümit Kara","doi":"10.1016/j.jrras.2025.102132","DOIUrl":"10.1016/j.jrras.2025.102132","url":null,"abstract":"<div><div>This study introduces an integrated approach combining forced degradation analysis and Monte Carlo gamma modeling to assess the radiosterilization suitability of pharmaceutical compounds. Three imidazole antifungals were subjected to controlled UV (365 nm) and oxidative (3 % H₂O₂) stress conditions, and their degradation behavior was quantitatively assessed using validated reversed phase high performance liquid chromatography (RP-HPLC), yielding percentage degradation, rate constants, and half-life values. UV degradation after 3h was highest for lanoconazole (70.97 %), followed by bifonazole (50.80 %) and butoconazole (15.44 %), confirming greater photostability for butoconazole. Under oxidative stress, bifonazole showed the highest resistance, while lanoconazole fully degraded within 12h. MCNPX Monte Carlo simulations revealed enhanced gamma attenuation and localized energy deposition for bifonazole, consistent with its experimental degradation resistance. Kerma and dose-rate analyses supported a balanced energy deposition profile for bifonazole, correlating with its experimental stability. The integration of chemical degradation profiling via RP-HPLC with physical interaction modeling via Monte Carlo simulations offers a predictive, quantitative framework for assessing radiosterilization tolerance. This approach reduces reliance on empirical irradiation trials, enables early identification of the most stable active pharmaceutical ingredients, and supports the optimization of sterilization protocols in pharmaceutical development. This framework enables early identification of radiation-stable APIs, minimizing empirical trials and supporting optimized sterilization strategies without compromising drug efficacy.</div></div>","PeriodicalId":16920,"journal":{"name":"Journal of Radiation Research and Applied Sciences","volume":"19 1","pages":"Article 102132"},"PeriodicalIF":2.5,"publicationDate":"2025-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145880525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Network pharmacology, molecular dynamics simulation, and experiments uncover icariin's role in ferroptosis during postmenopausal osteoporosis","authors":"Feng Chen ,&nbsp;Yueping Chen ,&nbsp;Xiaoyun Zhang","doi":"10.1016/j.jrras.2025.102147","DOIUrl":"10.1016/j.jrras.2025.102147","url":null,"abstract":"<div><h3>Background</h3><div>Icariin (ICA) shows therapeutic potential for postmenopausal osteoporosis (PMO), yet its mechanism in regulating osteoblast ferroptosis remains unclear. We combined network pharmacology, molecular docking, molecular dynamics simulation, and in vitro validation to elucidate ICA-mediated anti-ferroptotic effects in PMO.</div></div><div><h3>Methods</h3><div>Potential ICA targets were obtained from PubChem, the Comparative Toxicogenomics Database, SwissTargetPrediction, and the Similarity Ensemble Approach, while PMO-associated genes were identified through GeneCards, OMIM, DrugBank, and the Therapeutic Target Database. Ferroptosis-related genes were extracted from FerrDb. Intersecting targets were used to construct drug–target and protein–protein interaction (PPI) networks, and key nodes were identified by topological analysis. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were used to perform functional enrichment. Molecular docking assessed ICA affinity toward candidate proteins, and molecular dynamics simulations evaluated complex stability. Reactive oxygen species (ROS), apoptosis, and the expression of markers linked to ferroptosis, antioxidants, and osteogenesis were measured using osteoblasts in vitro using quantitative RT-PCR and western blotting.</div></div><div><h3>Results</h3><div>Eight key targets were identified through PPI network analysis. GO and KEGG enrichment analyses highlighted pathways associated with redox regulation and ferroptosis. Docking studies revealed the strongest binding affinity between ICA and Nrf2 (Nuclear Factor Erythroid 2–Related Factor 2), further confirmed by stable interactions in molecular dynamics simulations. Experimentally, ICA promoted osteoblast proliferation, reduced intracellular ROS levels and apoptosis, and upregulated antioxidant, osteogenic, and ferroptosis-related proteins, including Nrf2, heme oxygenase-1 (HO-1), glutathione peroxidase-4 (GPX4), solute carrier family 7 member 11 (SLC7A11), alkaline phosphatase (ALP), and runt-related transcription factor-2 (RUNX2), while suppressing caspase-3 activation.</div></div><div><h3>Conclusion</h3><div>ICA activates the Nrf2/HO-1 pathway to suppress ferroptosis and oxidative stress, reduce apoptosis, and promote osteogenic activity, thereby alleviating PMO-related osteoblast dysfunction. These findings provide mechanistic support for ICA as a multi-target candidate for PMO management.</div></div>","PeriodicalId":16920,"journal":{"name":"Journal of Radiation Research and Applied Sciences","volume":"19 1","pages":"Article 102147"},"PeriodicalIF":2.5,"publicationDate":"2025-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145880591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}