Pub Date : 2021-01-01DOI: 10.4103/jrptps.jrptps_22_21
Jaspreet Kaur, Parminder Nain, Sunil Kumar, M. Bhatia
{"title":"Enhancing cognitive performance with rejuvenation of brain antioxidant markers and acetylcholinesterase activity by ethanolic extract of Cucurbita pepo L. seeds in scopolamine-induced model of dementia in rats","authors":"Jaspreet Kaur, Parminder Nain, Sunil Kumar, M. Bhatia","doi":"10.4103/jrptps.jrptps_22_21","DOIUrl":"https://doi.org/10.4103/jrptps.jrptps_22_21","url":null,"abstract":"","PeriodicalId":16966,"journal":{"name":"Journal of Reports in Pharmaceutical Sciences","volume":"1 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70821494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Tin (IV) oxide (SnO2) nanoparticles inhibit the viability of cervical cancer HeLa cells through induction of apoptosis","authors":"E. Eftekhar, Parisa Bazsefidpar, Shabnaz Koochakkhani, Behnaz Rahnama Inchehsablagh, Elahe Aliasgari","doi":"10.4103/jrptps.jrptps_109_20","DOIUrl":"https://doi.org/10.4103/jrptps.jrptps_109_20","url":null,"abstract":"","PeriodicalId":16966,"journal":{"name":"Journal of Reports in Pharmaceutical Sciences","volume":"1 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70821539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.4103/jrptps.jrptps_12_20
Padmavathi Pamulapati, R. Birudu, Sathish Kumar Manoharan
{"title":"Effects of Aegle marmelos (L.) methanolic leaf extracts on biochemical parameters in diabetic rats","authors":"Padmavathi Pamulapati, R. Birudu, Sathish Kumar Manoharan","doi":"10.4103/jrptps.jrptps_12_20","DOIUrl":"https://doi.org/10.4103/jrptps.jrptps_12_20","url":null,"abstract":"","PeriodicalId":16966,"journal":{"name":"Journal of Reports in Pharmaceutical Sciences","volume":"1 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70821678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-07-01DOI: 10.4103/jrptps.JRPTPS_23_19
Hayam S Ahmed, A. Moawad, A. Owis, S. AbouZid
Background: Calligonum polygonoides subsp. comosum is a perennial desert plant. Most of the previous chemical investigation of this plant was performed on the whole herb but there were no data about quantification of active constituent in different organs of C. polygonoides. Materials and Methods: in vitro antioxidant activity, total phenolic, and total flavonoid contents of the different organs were determined using 2, 2-diphenyl-1-picrylhydrazyl (DPPH), Folin–Ciocalteu, and aluminum chloride (AlCl3) methods, respectively. Quantitative analysis of the phenolic compounds was determined in the different organs of the plant using high-performance liquid chromatography (HPLC). Results: Both bark and leaves showed the highest radical scavenging activity with the values of 450.30 and 398.10 μg/g ascorbic acid equivalent, respectively. The total phenolic content of the samples was in the range of 27.9–281.5 μg/g gallic acid equivalent and total flavonoid content of the samples was in the range of 53.9–257.4 μg/g rutin equivalent where the leaves and bark showed the highest contents. HPLC analysis showed that flavonol glycosides content was higher in all organs compared to the aglycones. Flowers and fruits were the richest organs in flavonols, whereas leaves, stems, and bark were the richest in taxifolin and catechin. Conclusion: Depending on the obtained results C. polygonoides is an excellent source of natural antioxidants.
{"title":"Antioxidant capacity and HPLC determination of phenolic in different organs of Calligonum polygonoides subspecies comosum","authors":"Hayam S Ahmed, A. Moawad, A. Owis, S. AbouZid","doi":"10.4103/jrptps.JRPTPS_23_19","DOIUrl":"https://doi.org/10.4103/jrptps.JRPTPS_23_19","url":null,"abstract":"Background: Calligonum polygonoides subsp. comosum is a perennial desert plant. Most of the previous chemical investigation of this plant was performed on the whole herb but there were no data about quantification of active constituent in different organs of C. polygonoides. Materials and Methods: in vitro antioxidant activity, total phenolic, and total flavonoid contents of the different organs were determined using 2, 2-diphenyl-1-picrylhydrazyl (DPPH), Folin–Ciocalteu, and aluminum chloride (AlCl3) methods, respectively. Quantitative analysis of the phenolic compounds was determined in the different organs of the plant using high-performance liquid chromatography (HPLC). Results: Both bark and leaves showed the highest radical scavenging activity with the values of 450.30 and 398.10 μg/g ascorbic acid equivalent, respectively. The total phenolic content of the samples was in the range of 27.9–281.5 μg/g gallic acid equivalent and total flavonoid content of the samples was in the range of 53.9–257.4 μg/g rutin equivalent where the leaves and bark showed the highest contents. HPLC analysis showed that flavonol glycosides content was higher in all organs compared to the aglycones. Flowers and fruits were the richest organs in flavonols, whereas leaves, stems, and bark were the richest in taxifolin and catechin. Conclusion: Depending on the obtained results C. polygonoides is an excellent source of natural antioxidants.","PeriodicalId":16966,"journal":{"name":"Journal of Reports in Pharmaceutical Sciences","volume":"9 1","pages":"251 - 255"},"PeriodicalIF":0.6,"publicationDate":"2020-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42609303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-07-01DOI: 10.4103/jrptps.JRPTPS_98_19
Abbas Abbasi, A. Azizi, Seidmostafa Nachvak, E. Alizadeh, R. Abbsavaran, E. Mirtaheri, Mehdi Moradinazar, M. Rahimi
Aim: Ginger is a natural dietary rhizome with antioxidant, anti-inflammatory, and anticarcinogenic properties. It has many medical beneficial properties such as anti-proliferation and antiapoptotic effects on cancerous esophageal cells. Materials and Methods: Esophageal cancer cells ESO26 were cultured in the presence and absence of ginger extract at various concentrations for 12, 18, and 24h. Then, the viability was determined by 3-(4,5-dimethylthiazol)-2,5-diphenyl tetrazolium bromide (MTT) assay. Western blot analysis of caspase-3 was performed to detect apoptosis. p21, Bax, and Bcl-2 gene expression was measured using quantitative polymerase chain reaction (PCR). Data were analyzed using one-way analysis of variance (ANOVA) followed by Tukey test. Results: The ginger extract increased the cleavage of caspase-3 in cells (P < 0.05). Results of real-time PCR have shown that ginger decreased the expression of Bcl-2 and increased Bax and p21 gene expression (P < 0.05). Conclusions: Results showed that the process of cell proliferation has been stopped. Also, this study indicated that ginger might exert a chemopreventive effect on esophageal cancer through the suppression of proliferation and the growth of tumor cells as well as the induction of apoptosis.
{"title":"Apoptotic effects of ginger extract (Zingiber officinale) on esophageal cancer cells ESO26: An in vitro study","authors":"Abbas Abbasi, A. Azizi, Seidmostafa Nachvak, E. Alizadeh, R. Abbsavaran, E. Mirtaheri, Mehdi Moradinazar, M. Rahimi","doi":"10.4103/jrptps.JRPTPS_98_19","DOIUrl":"https://doi.org/10.4103/jrptps.JRPTPS_98_19","url":null,"abstract":"Aim: Ginger is a natural dietary rhizome with antioxidant, anti-inflammatory, and anticarcinogenic properties. It has many medical beneficial properties such as anti-proliferation and antiapoptotic effects on cancerous esophageal cells. Materials and Methods: Esophageal cancer cells ESO26 were cultured in the presence and absence of ginger extract at various concentrations for 12, 18, and 24h. Then, the viability was determined by 3-(4,5-dimethylthiazol)-2,5-diphenyl tetrazolium bromide (MTT) assay. Western blot analysis of caspase-3 was performed to detect apoptosis. p21, Bax, and Bcl-2 gene expression was measured using quantitative polymerase chain reaction (PCR). Data were analyzed using one-way analysis of variance (ANOVA) followed by Tukey test. Results: The ginger extract increased the cleavage of caspase-3 in cells (P < 0.05). Results of real-time PCR have shown that ginger decreased the expression of Bcl-2 and increased Bax and p21 gene expression (P < 0.05). Conclusions: Results showed that the process of cell proliferation has been stopped. Also, this study indicated that ginger might exert a chemopreventive effect on esophageal cancer through the suppression of proliferation and the growth of tumor cells as well as the induction of apoptosis.","PeriodicalId":16966,"journal":{"name":"Journal of Reports in Pharmaceutical Sciences","volume":"9 1","pages":"183 - 188"},"PeriodicalIF":0.6,"publicationDate":"2020-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49489997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-07-01DOI: 10.4103/jrptps.JRPTPS_70_19
F. Dar, Tanveer Bilal Pirzadah, I. Tahir, R. Rehman
Context: The Kashmir and Ladakh Himalayan regions are having a rich diversity of buckwheat germplasm, which is an excellent source of nutrition and functional food. The objective of this study was based on comparative in vitro flavonoid, antioxidant, and mineral analyses of Fagopyrum species grown in these regions. Materials and Methods: To achieve this goal, leaf samples from the four buckwheat species were subjected to antioxidant analysis. Besides, the mineral analysis of the groat samples of different buckwheat species was carried out by atomic absorption spectroscopy (AAS). Results: Results indicated that the methanolic extract shows higher total phenolic content (TPC) and total flavonoid content (TFC) in the samples of Fagopyrum sagittatum followed by Fagopyrum tataricum, Fagopyrum kashmirianum, and Fagopyrum esculentum. Total reducing power (TRP), ferric reducing antioxidant power (FRAP), 1,1-diphenyl-2-picrylhydrazyl (DPPH), superoxide anion radical (SOR), and hydrogen peroxide (H2O2) radical scavenging assays indicated excellent results from the leaf extracts of F. sagittatum. The results suggested that the crude methanolic extract of buckwheat species had effective reducing power, radical scavenging activity, and metal-chelating ability compared to other standard antioxidants. AAS analysis revealed that calcium content was higher in F. sagittatum (21,600 ppm), whereas the iron and zinc contents were higher in F. kashmirianum (1,122.5 ppm) and F. sagittatum (166.75 ppm), respectively. Conclusion: Our study suggested that methanolic extracts of Fagopyrum species could act as a potent source of natural antioxidants to the pharmaceutical and food industry. In addition, the study also revealed that the rich elemental profiles of buckwheat species specify their therapeutic value and thus could be used as a potential biofortification crop.
{"title":"Deciphering the in vitro antioxidant potential and mineral analysis of Fagopyrum species from Kashmir and Ladakh regions","authors":"F. Dar, Tanveer Bilal Pirzadah, I. Tahir, R. Rehman","doi":"10.4103/jrptps.JRPTPS_70_19","DOIUrl":"https://doi.org/10.4103/jrptps.JRPTPS_70_19","url":null,"abstract":"Context: The Kashmir and Ladakh Himalayan regions are having a rich diversity of buckwheat germplasm, which is an excellent source of nutrition and functional food. The objective of this study was based on comparative in vitro flavonoid, antioxidant, and mineral analyses of Fagopyrum species grown in these regions. Materials and Methods: To achieve this goal, leaf samples from the four buckwheat species were subjected to antioxidant analysis. Besides, the mineral analysis of the groat samples of different buckwheat species was carried out by atomic absorption spectroscopy (AAS). Results: Results indicated that the methanolic extract shows higher total phenolic content (TPC) and total flavonoid content (TFC) in the samples of Fagopyrum sagittatum followed by Fagopyrum tataricum, Fagopyrum kashmirianum, and Fagopyrum esculentum. Total reducing power (TRP), ferric reducing antioxidant power (FRAP), 1,1-diphenyl-2-picrylhydrazyl (DPPH), superoxide anion radical (SOR), and hydrogen peroxide (H2O2) radical scavenging assays indicated excellent results from the leaf extracts of F. sagittatum. The results suggested that the crude methanolic extract of buckwheat species had effective reducing power, radical scavenging activity, and metal-chelating ability compared to other standard antioxidants. AAS analysis revealed that calcium content was higher in F. sagittatum (21,600 ppm), whereas the iron and zinc contents were higher in F. kashmirianum (1,122.5 ppm) and F. sagittatum (166.75 ppm), respectively. Conclusion: Our study suggested that methanolic extracts of Fagopyrum species could act as a potent source of natural antioxidants to the pharmaceutical and food industry. In addition, the study also revealed that the rich elemental profiles of buckwheat species specify their therapeutic value and thus could be used as a potential biofortification crop.","PeriodicalId":16966,"journal":{"name":"Journal of Reports in Pharmaceutical Sciences","volume":"9 1","pages":"235 - 245"},"PeriodicalIF":0.6,"publicationDate":"2020-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41750604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-07-01DOI: 10.4103/jrptps.JRPTPS_57_19
Afshin Ramian, I. Javadi, H. Sadeghi, H. Sadeghi, E. Panahi kokhdan, A. Doustimotlagh, R. Abbasi, S. Alizadeh, Hamed Nikbakht
Background: Depression is one of the most prevalent and life-threatening forms of mental disorders in chronic kidney disease. Antidepressant agents such as fluvoxamine are broadly prescribed in this situation. This study investigated the effects of fluvoxamine on gentamicin (GEN)-induced nephrotoxicity in rats. Materials and Methods: Twenty-four male Wistar rats were randomly divided into four groups (n = 6) including (1) control group, (2) GEN group, (3) GEN + fluvoxamine (25 mg/kg) group, and (4) GEN + fluvoxamine (50 mg/kg) group. Fluvoxamine was orally given to animals 45 min before GEN was injected (100 mg/kg, intraperitoneally [i.p.]). Blood urea nitrogen (BUN), creatinine (Cr), sodium (Na+), potassium (K+), and malondialdehyde (MDA) levels in serum were measured. Moreover, the glucose (Glu) and protein (Pro) levels in urine and the ratio of kidney to body weight (g/100g body weight) were determined. Histopathological alterations in kidney were evaluated. Results: GEN significantly increased the Cr and BUN serum levels as well as urine Glu and Pro concentrations (P ≤ 0.001). Fluvoxamine exacerbated the elevation in the indicated parameters. GEN also significantly increased the serum MDA levels. Fluvoxamine had no effect on the elevated serum levels of MDA. GEN did not show any effect on the K+ and Na+ serum concentrations. Increased kidney-to-body weight ratio due to GEN nephrotoxicity was further exacerbated by 25 mg/kg of fluvoxamine (P ≤ 0.001). Pathologic findings also confirm the biochemical results. Conclusion: The data suggest that fluvoxamine worsens the nephrotoxicity of GEN. However, further clinical and animal investigations are required to elucidate the mechanism of this interaction.
{"title":"Repeated administration of fluvoxamine worsens gentamicin-induced nephrotoxicity in rats","authors":"Afshin Ramian, I. Javadi, H. Sadeghi, H. Sadeghi, E. Panahi kokhdan, A. Doustimotlagh, R. Abbasi, S. Alizadeh, Hamed Nikbakht","doi":"10.4103/jrptps.JRPTPS_57_19","DOIUrl":"https://doi.org/10.4103/jrptps.JRPTPS_57_19","url":null,"abstract":"Background: Depression is one of the most prevalent and life-threatening forms of mental disorders in chronic kidney disease. Antidepressant agents such as fluvoxamine are broadly prescribed in this situation. This study investigated the effects of fluvoxamine on gentamicin (GEN)-induced nephrotoxicity in rats. Materials and Methods: Twenty-four male Wistar rats were randomly divided into four groups (n = 6) including (1) control group, (2) GEN group, (3) GEN + fluvoxamine (25 mg/kg) group, and (4) GEN + fluvoxamine (50 mg/kg) group. Fluvoxamine was orally given to animals 45 min before GEN was injected (100 mg/kg, intraperitoneally [i.p.]). Blood urea nitrogen (BUN), creatinine (Cr), sodium (Na+), potassium (K+), and malondialdehyde (MDA) levels in serum were measured. Moreover, the glucose (Glu) and protein (Pro) levels in urine and the ratio of kidney to body weight (g/100g body weight) were determined. Histopathological alterations in kidney were evaluated. Results: GEN significantly increased the Cr and BUN serum levels as well as urine Glu and Pro concentrations (P ≤ 0.001). Fluvoxamine exacerbated the elevation in the indicated parameters. GEN also significantly increased the serum MDA levels. Fluvoxamine had no effect on the elevated serum levels of MDA. GEN did not show any effect on the K+ and Na+ serum concentrations. Increased kidney-to-body weight ratio due to GEN nephrotoxicity was further exacerbated by 25 mg/kg of fluvoxamine (P ≤ 0.001). Pathologic findings also confirm the biochemical results. Conclusion: The data suggest that fluvoxamine worsens the nephrotoxicity of GEN. However, further clinical and animal investigations are required to elucidate the mechanism of this interaction.","PeriodicalId":16966,"journal":{"name":"Journal of Reports in Pharmaceutical Sciences","volume":"9 1","pages":"196 - 202"},"PeriodicalIF":0.6,"publicationDate":"2020-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44293981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-07-01DOI: 10.4103/jrptps.JRPTPS_64_18
Zeynab Zarrabi, L. Saghaie, A. Fassihi, N. Pestechian, S. Saberi
Background: Leishmaniasis infection threatens millions of people in under developing and developing countries. Treatment of this neglected disease is very complicated. Subjects and Methods: A novel series of antimony (V) complexes using bidentate ligands of hydroxypyranones and hydroxypyridinones have been designed and synthesized. For the synthesis of the complexes, SbCl5 in water was added to the solution of each ligand at 60°C and the pH of mixture was adjusted to 8 using aqueous NaOH. After 24 h stirring, extraction of produced compound into acetone gave the desired complex. The structure of complexes was achieved by using FTIR, 1HNMR, and electron spin ionization mass spectroscopic techniques. All compounds were evaluated for in vitro anti amastogote form of Leishmania major. Results and Conclusion: The most potent antimony complexes against amastigotes were 5b (after 48 and 72 h) and 5a (after 72 h) with IC50 values of 24.4, 16.3, and 30.1 µg/mL, respectively. Furthermore, antimony and iron complexes were used together for in vitro anti amastigote form of L. major activity. These compounds were toxic for macrophages and destroyed them.
{"title":"Synthesis and comparison of anti-Leishmania major activity of antimony and iron complexes of 3-hydroxypyran-4-one and 3-hydroxypyridine-4-one as bi-dentate ligands","authors":"Zeynab Zarrabi, L. Saghaie, A. Fassihi, N. Pestechian, S. Saberi","doi":"10.4103/jrptps.JRPTPS_64_18","DOIUrl":"https://doi.org/10.4103/jrptps.JRPTPS_64_18","url":null,"abstract":"Background: Leishmaniasis infection threatens millions of people in under developing and developing countries. Treatment of this neglected disease is very complicated. Subjects and Methods: A novel series of antimony (V) complexes using bidentate ligands of hydroxypyranones and hydroxypyridinones have been designed and synthesized. For the synthesis of the complexes, SbCl5 in water was added to the solution of each ligand at 60°C and the pH of mixture was adjusted to 8 using aqueous NaOH. After 24 h stirring, extraction of produced compound into acetone gave the desired complex. The structure of complexes was achieved by using FTIR, 1HNMR, and electron spin ionization mass spectroscopic techniques. All compounds were evaluated for in vitro anti amastogote form of Leishmania major. Results and Conclusion: The most potent antimony complexes against amastigotes were 5b (after 48 and 72 h) and 5a (after 72 h) with IC50 values of 24.4, 16.3, and 30.1 µg/mL, respectively. Furthermore, antimony and iron complexes were used together for in vitro anti amastigote form of L. major activity. These compounds were toxic for macrophages and destroyed them.","PeriodicalId":16966,"journal":{"name":"Journal of Reports in Pharmaceutical Sciences","volume":"9 1","pages":"177 - 182"},"PeriodicalIF":0.6,"publicationDate":"2020-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43434269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-07-01DOI: 10.4103/jrptps.JRPTPS_71_19
F. Bayat, Atefeh Hajiagha Bozorgi
In this study, a hollow fiber liquid-phase microextraction (HF-LPME) method coupled with high-performance liquid chromatography (HPLC) was successfully developed for the determination of trace levels of lovastatin in urine and plasma samples. Lovastatin was extracted from 15 mL of the acidic sample solution with a pH of 2 into an organic extracting solvent (n-octanol) impregnated in the pores of a hollow fiber and then back extracted into an acidified aqueous solution in the lumen of the hollow fiber. After extraction, 10 µL of the acceptor phase was injected into HPLC system. To obtain high extraction efficiency, the parameters affecting the HF-LPME, including pH of the sample and extractant phases, type of organic phase, ionic strength, stirring rate, extraction time, and temperature, were studied and optimized. Under the optimized conditions (solvent 1-octanol, pH = 2, 45 min stirring at 45°C with 750rpm), the relative recovery percentage was 85.2–97, which shows the capability of the method to analyze the analyte concentration. This technique provided preconcentration factor 199, 185, and 170 for water, urine, and plasma, respectively. Good precisions values (with relative standard division ≤ 10.5%) were obtained. The results indicated that the HF-LPME method has an excellent cleanup capacity and a high preconcentration factor and could serve as a simple and sensitive method for monitoring the drug in biological samples.
本研究成功建立了一种中空纤维液相微萃取(HF-LPME) -高效液相色谱(HPLC)联用法测定尿和血浆样品中微量洛伐他汀的方法。将洛伐他汀从15 mL pH为2的酸性样品溶液中提取到浸渍在中空纤维孔隙中的有机提取溶剂(正辛醇)中，然后再将其提取到中空纤维管腔中的酸化水溶液中。提取后，将受体相10µL注入高效液相色谱系统。为了获得较高的萃取效率，研究并优化了影响HF-LPME的参数，包括样品和萃取剂的pH、有机相类型、离子强度、搅拌速率、萃取时间和温度。在优化条件下(溶剂为1-辛醇，pH = 2, 45℃，750rpm搅拌45 min)，相对回收率为85.2 - 97%，表明该方法具有分析分析被分析物浓度的能力。该技术对水、尿和血浆的预富集因子分别为199、185和170。获得了较好的精密度值(相对标准分割≤10.5%)。结果表明，HF-LPME方法具有良好的清除能力和较高的富集系数，可作为一种简便、灵敏的生物样品中药物监测方法。
{"title":"Hollow fiber-based liquid-phase microextraction and HPLC-UV determination of lovastatin in biological fluids","authors":"F. Bayat, Atefeh Hajiagha Bozorgi","doi":"10.4103/jrptps.JRPTPS_71_19","DOIUrl":"https://doi.org/10.4103/jrptps.JRPTPS_71_19","url":null,"abstract":"In this study, a hollow fiber liquid-phase microextraction (HF-LPME) method coupled with high-performance liquid chromatography (HPLC) was successfully developed for the determination of trace levels of lovastatin in urine and plasma samples. Lovastatin was extracted from 15 mL of the acidic sample solution with a pH of 2 into an organic extracting solvent (n-octanol) impregnated in the pores of a hollow fiber and then back extracted into an acidified aqueous solution in the lumen of the hollow fiber. After extraction, 10 µL of the acceptor phase was injected into HPLC system. To obtain high extraction efficiency, the parameters affecting the HF-LPME, including pH of the sample and extractant phases, type of organic phase, ionic strength, stirring rate, extraction time, and temperature, were studied and optimized. Under the optimized conditions (solvent 1-octanol, pH = 2, 45 min stirring at 45°C with 750rpm), the relative recovery percentage was 85.2–97, which shows the capability of the method to analyze the analyte concentration. This technique provided preconcentration factor 199, 185, and 170 for water, urine, and plasma, respectively. Good precisions values (with relative standard division ≤ 10.5%) were obtained. The results indicated that the HF-LPME method has an excellent cleanup capacity and a high preconcentration factor and could serve as a simple and sensitive method for monitoring the drug in biological samples.","PeriodicalId":16966,"journal":{"name":"Journal of Reports in Pharmaceutical Sciences","volume":"9 1","pages":"203 - 208"},"PeriodicalIF":0.6,"publicationDate":"2020-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47993107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-07-01DOI: 10.4103/jrptps.JRPTPS_99_19
Amandeep, S. Bhatt, Manish Kumar, S. Devi, P. Upadhyay, V. Saini, Amit Mittal, N. Mehan, Anupam Saini
Topical fungal infections are one of the often faced diseases worldwide. The first choice for the treatment of fungal infection is topical therapy due to its advantages such as decreasing the risk of systemic side effects and targeting the drug at the site of fungal infection. The treatment of the fungal infection depends on the penetration of the drug molecules through the outermost layer of the skin (stratum corneum) at an effective concentration. The disadvantages of topical treatment are its lack of drug adherence at the site of application and bigger particle size of drug molecules. Nanostructured lipid carriers are the advanced form of lipid nanoparticles and carry the nano range of the drug molecules, which helps to achieve localized and slow release. The topical route is generally preferred due to the possible side effects of oral medication. Advances in the field of formulation may soon render outdated conventional products such as creams, ointments, and gels. Several carrier systems loaded with antifungal drugs have shown promising results in the treatment of skin fungal infections.
{"title":"Recent advances in the development of the nanostructured lipid carriers for the topical fungal infections","authors":"Amandeep, S. Bhatt, Manish Kumar, S. Devi, P. Upadhyay, V. Saini, Amit Mittal, N. Mehan, Anupam Saini","doi":"10.4103/jrptps.JRPTPS_99_19","DOIUrl":"https://doi.org/10.4103/jrptps.JRPTPS_99_19","url":null,"abstract":"Topical fungal infections are one of the often faced diseases worldwide. The first choice for the treatment of fungal infection is topical therapy due to its advantages such as decreasing the risk of systemic side effects and targeting the drug at the site of fungal infection. The treatment of the fungal infection depends on the penetration of the drug molecules through the outermost layer of the skin (stratum corneum) at an effective concentration. The disadvantages of topical treatment are its lack of drug adherence at the site of application and bigger particle size of drug molecules. Nanostructured lipid carriers are the advanced form of lipid nanoparticles and carry the nano range of the drug molecules, which helps to achieve localized and slow release. The topical route is generally preferred due to the possible side effects of oral medication. Advances in the field of formulation may soon render outdated conventional products such as creams, ointments, and gels. Several carrier systems loaded with antifungal drugs have shown promising results in the treatment of skin fungal infections.","PeriodicalId":16966,"journal":{"name":"Journal of Reports in Pharmaceutical Sciences","volume":"9 1","pages":"271 - 278"},"PeriodicalIF":0.6,"publicationDate":"2020-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47908599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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