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Synthesis and in vitro Biological Studies of Copper Complexes Derived from Some Novel Hetero-Organoselanylquinoline Ligands 新型杂有机基喹啉配体铜配合物的合成及体外生物学研究
Pub Date : 2022-05-01 DOI: 10.3329/jsr.v14i2.56544
P. Moohambihai, K. Nagashri
A set of copper(II) complexes (3a-i) comprises (16E)-N-(2-phenylquinolin-4(1H)-ylidene)-3-(phenlselanyl)pyridine-2-amine (L1-L3), (16E)-2,6-dimethyl-N-(2-phenylquinolin-4(1H)-ylidene)-5-(phenlselanyl)pyrimidine-4-amine (L4-L6), 16E)-N-(2-phenylquinolin-4(1H)-ylidene)-2-(phenylselanyl)H-imidazo[1,2-α]pyridine-3-amine (L7-L9) ligands were synthesised and characterised using spectroscopic techniques. The metal-to-ligand 1:1 stoichiometry of prepared copper complexes was confirmed by mass spectra. The Cu(II) ion produces complexes with a distorted square planar, according to UV–Vis and ESR spectroscopy studies. The antioxidant activity of these complexes has been determined. The cytotoxic potential of the complexes was also investigated in vitro. The complex 3f was found to have significant cytotoxicity against MCF-7. The DNA binding characteristics of copper(II) complexes were investigated. The findings indicate that the complexes interacted with the calf thymus (CT-DNA). In addition, the complexes were tested in vitro for antimicrobial activity against three Gram-negative bacteria, three Gram-positive bacteria, and three Fungi.
合成了一组铜(II)配合物(3a-i),包括(16E)- n-(2-苯基喹啉-4(1H)-酰基)-3-(苯selanyl)吡啶-2-胺(L1-L3), (16E)-2,6-二甲基- n-(2-苯基喹啉-4(1H)-酰基)-5-(苯selanyl)嘧啶-4-胺(L4-L6), 16E)- n-(2-苯基喹啉-4(1H)-酰基)-2-(苯selanyl) h -咪唑[1,2-α]吡啶-3-胺(L7-L9)配体,并利用光谱技术进行了表征。质谱分析证实了铜配合物的金属-配体1:1的化学计量。根据紫外-可见和ESR光谱研究,Cu(II)离子产生具有扭曲方形平面的配合物。测定了这些配合物的抗氧化活性。体外研究了该复合物的细胞毒性潜能。发现复合物3f对MCF-7具有显著的细胞毒性。研究了铜(II)配合物的DNA结合特性。研究结果表明,这些复合物与小牛胸腺(CT-DNA)相互作用。此外,还对三种革兰氏阴性菌、三种革兰氏阳性菌和三种真菌进行了体外抗菌活性测试。
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引用次数: 0
On Certain Quadruple Fixed Points of Integral Type Contraction Mappings in G-Metric Spaces and its Applications g -度量空间中积分型收缩映射的若干四重不动点及其应用
Pub Date : 2022-05-01 DOI: 10.3329/jsr.v14i2.56189
B. Rao, L. V. Kumar, C. Maheswari, S. M. Shaw
In this paper, the existence of common quadruple fixed point results for integral type contraction of two mappings in G-metric spaces is established. Some interesting consequences of our results are achieved. Moreover, we give an illustration that presents the applicability of the achieved results.
本文建立了g -度量空间中两个映射的积分型收缩的公共四重不动点结果的存在性。我们的结果产生了一些有趣的结果。此外,我们给出了一个例子来说明所取得的结果的适用性。
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引用次数: 0
Modeling of the Peristaltic Lithogeic Bile Flow in the Calculus Duct Under the Influence of Heat Transfer with Slip Boundary Conditions 滑移边界条件下换热影响下结石管内岩石胆汁蠕动流动的模拟
Pub Date : 2022-05-01 DOI: 10.3329/jsr.v14i2.55882
T. Rawat, S. Kumari, S. Singh
This paper deals with a mathematical model that represents non-Newtonian bile flow through a calculus duct under the influence of heat transfer with wall slip conditions. The peristaltic flow of bile is characterized by a generalized Carreau's model. An ordinate transformation is initiated to map the cosine geometry of the stone into a rectangular grid. It allows evaluation velocity, pressure distribution, flow rate, and reflux occurrence conditions, adopting the perturbation technique, the analytical solution obtained under various parameters, such as Knudsen number, amplitude ratio, Grashof number, Weissenberg number, and power index. These mathematical expressions are analyzed by plotting the graph in MATLAB R2018b software and observed that the axial velocity and the pressure gradient are strongly affected by heat and wall slip parameters.
本文讨论了在壁面滑移条件下传热影响下非牛顿胆汁流过结石管的数学模型。胆汁的蠕动流动是用广义的卡鲁模型来描述的。开始进行纵坐标变换,将石头的余弦几何映射到矩形网格中。它可以评价流速、压力分布、流量和回流发生条件,采用摄动技术,得到各种参数下的解析解,如Knudsen数、振幅比、Grashof数、Weissenberg数和功率指数。在MATLAB R2018b软件中对这些数学表达式进行了分析,并观察到轴向速度和压力梯度受热量和壁面滑移参数的强烈影响。
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引用次数: 0
GC-MS, Phytochemical Analysis and In Silico Approaches of a Medicinal Plant Acalypha indica 药用植物白果的气相色谱-质谱、植物化学分析及计算机方法研究
Pub Date : 2022-05-01 DOI: 10.3329/jsr.v14i2.56648
P. Rajkumar, S. Sundari, S. Selvaraj, A. Natarajan, R. Suganya, R. Jayaprakash, K. Kasthuri, S. Kumaresan
The goal of this study is to look into the preliminary phytochemical and GC-MS analyses of a methanol extract of the leaves of Acalypha indica. Alkaloids, tannins, steroids, saponins, flavonoids, glycosides, and phenolic substances are found in leaf extract after phytochemical analysis. A. indica is a plant that grows in the southern Indian state of Tamil Nadu. It has medicinal characteristics and is used as a diuretic, anthelmintic, and respiratory disorders like bronchitis, asthma, and pneumonia. The present work is designed to investigate GC-MS. Thirty compounds are found in the leaf extract. The structure-based biological activities study supported the Pass online database findings from the current study support that the use of this plant compound responsible for the anticancer activity is based on structure-based drug development. Based on in-silico results, it was shown that the interactions of hydrogen bonds, binding affinity with JAK2 kinase inhibitors, tumor suppressor proteins pRB Retinoblastoma protein, and COX-2 target proteins were compared with known values. It can be considered an increase in the possibility of conniving potential anticancer drugs as an anti-inflammatory agent.
本研究的目的是初步研究白果叶甲醇提取物的植物化学和气相色谱-质谱分析。经植物化学分析,叶提取物中含有生物碱、单宁、甾体、皂苷、黄酮类、苷类和酚类物质。印度藜是一种生长在印度南部泰米尔纳德邦的植物。它具有药用特性,被用作利尿剂、驱虫药和呼吸系统疾病,如支气管炎、哮喘和肺炎。本工作旨在研究GC-MS。在叶提取物中发现了30种化合物。基于结构的生物活性研究支持了Pass在线数据库的研究结果,目前的研究结果支持这种植物化合物的抗癌活性的使用是基于结构的药物开发。基于in-silico结果,我们发现氢键的相互作用、与JAK2激酶抑制剂、肿瘤抑制蛋白pRB视网膜母细胞瘤蛋白和COX-2靶蛋白的结合亲和力与已知值进行了比较。它可以被认为增加了潜在抗癌药物作为抗炎剂的可能性。
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引用次数: 3
Transgenic Peanut (Arachis hypogaea L.) Plants Conferring Enhanced Protection Against Fungal Pathogens by Expressing Tc chitinase-I Gene 转基因花生(arachhis hypogaea L.)植物表达Tc几丁质酶- 1基因增强对真菌病原体的保护作用
Pub Date : 2022-05-01 DOI: 10.3329/jsr.v14i2.56542
R. Marka, G. S. Malothu, R. Nanna
In this study, an efficient Agrobacterium-mediated genetic transformation method followed by Tc chitiI gene for enhanced expression in peanut (Arachis hypogaea L.) cv ICG 7827 is reported. A. tumefaciens strain LBA 4404 harboring the plant transformation plasmid pBinAR contains the Tc chitI gene and nptII gene as a selectable marker. The precultured leaflet (LL) explants were infected with A. tumefaciens containing Tc chitiI gene and cocultivated on SIM (MMS+10 mg/L BAP+1 mg/L NAA+2 mg/L AgNO3) for four days. After co-cultivation, these explants were transferred to selection medium (SIM+75 mg/L Kan+250 mg/L Cefotaxime). In T0 generation, 70 % of transformation efficiency was recorded. T1 generation derived from the primary transgenic (T0) events revealed a Mendelian inheritance pattern (3:1) for the Tc chit I transgene. T1 transgenic peanut plants were tested for resistance against C. arachidicola, C. personatum, and P. arachidis by infection with the microspores using detached leaf assay. The Tc chitI gene expressed in T1 transgenic plants have shown longer incubation, longer latent period, and lower infection frequencies than non-transformed (WT) plants. A significant negative correlation was recorded between chitinase activity and the frequency of infection to the C. arachidicola, C. personatum and P. arachidis pathogens.
本研究报道了一种高效的农杆菌介导的花生ICG 7827基因转入tcchitii基因的方法。携带植物转化质粒pBinAR的a . tummefaciens菌株LBA 4404含有Tc chitI基因和nptII基因作为选择性标记。将预培养的小叶(LL)外植体感染含有Tc chitiI基因的瘤胃芽孢杆菌,在SIM (MMS+10 mg/L BAP+1 mg/L NAA+2 mg/L AgNO3)上共培养4 d。共培养后移栽至选择培养基(SIM+75 mg/L Kan+250 mg/L头孢噻肟)。在第0代中,记录了70%的转换效率。由原代转基因(T0)事件衍生的T1代显示了Tc chit I转基因的孟德尔遗传模式(3:1)。采用离叶法对T1转基因花生植株进行小孢子侵染,检测其对花生花生小孢子虫、人花生小孢子虫和花生花生小孢子虫的抗性。T1转基因植株中表达的tcchiti基因比未转化植株的潜伏期长、潜伏期长、侵染率低。几丁质酶活性与花生achiidicola、C. personatum和P. arachiidis病原菌感染频率呈显著负相关。
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引用次数: 0
Total Phenolic Contents and Anti-lipooxygenase Activity of the Methanolic Extracts from Durio zibethinus Murr 紫豆醇提物总酚含量及抗脂氧化酶活性研究
Pub Date : 2022-05-01 DOI: 10.3329/jsr.v14i2.56287
S. Adeniyi, G. Olatunji, O. Oguntoye
Durio zibethinus parts (leaves, stem bark, and root) used to treat malaria fever, vermifuge, diabetes, jaundice, inflammation, and oxidative stress management prompted the investigation of their total phenolic contents and anti-lipooxygenase activities. The total phenolic concentration of D. zibethinus parts was determined spectrophotometrically after collection and extraction. The anti-inflammatory activity was estimated using a lipooxygenase assay. Total phenolic content measured in gallic acid equivalent (GAE) gave the highest levels of 6.88 ± 1.54, 23.32 ± 1.73, and 29.00 ± 3.43 mg GAE/g sample weight leaves stem bark, and root extracts, respectively. The plant extracts showed lower anti- lipooxygenase activity in leaves (IC50 µg/mL- 1.464) and stem bark (IC50 µg/mL- 1.203), but had significant activity in root (IC50 µg/mL- 1.400) compared to standard indomethacin (IC50 µg/mL- 1.660). The plant extract's declining order of anti- lipooxygenase activity follows the same trend with the phenolic contents, indicating that the extract's magnitude of anti- lipooxygenase activity is comparable with the quantity of phenolic compounds present in the extracts. Hence, the D. zibethinus extracts are a potential candidate for a plant-derived anti-inflammatory agent.
紫豆部分(叶、茎、皮和根)用于治疗疟疾热、寄生虫病、糖尿病、黄疸、炎症和氧化应激管理,促使人们对其总酚含量和抗脂氧化酶活性进行研究。收集提取后,采用分光光度法测定了紫荆各部位总酚的含量。用脂氧化酶测定法估计其抗炎活性。未食子酸当量(GAE)中总酚含量最高,分别为6.88±1.54、23.32±1.73和29.00±3.43 mg GAE/g样品质量。与标准吲哚美辛(IC50µg/mL- 1.660)相比,该植物提取物在叶片(IC50µg/mL- 1.464)和茎皮(IC50µg/mL- 1.203)中的抗脂氧化酶活性较低,但在根(IC50µg/mL- 1.400)中的抗脂氧化酶活性显著。植物提取物抗脂氧化酶活性的下降顺序与酚类化合物含量的下降趋势一致,表明提取物抗脂氧化酶活性的强弱与酚类化合物含量相当。因此,zibethinus提取物是一种潜在的候选植物源抗炎剂。
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引用次数: 0
Video Summarization Using Deep Learning for Cricket Highlights Generation 使用深度学习生成板球集锦的视频摘要
Pub Date : 2022-05-01 DOI: 10.3329/jsr.v14i2.56856
D. Gaikwad, S. Sarap, D. Dhande
Recently, video surveillance technology has grown pervasive in many aspects of our lives. Automatic video monitoring produces massive amounts of data that need human examination at some point. The primary emphasis is on reducing storage usage by compressing or eliminating superfluous frames without sacrificing real information. The current effort seeks to close the growing gap between the amounts of real data and the volume. Searching through key events in large video collections is time-consuming and tedious. In this paper, smart surveillance for various applications by using video summarization has been presented. A method for generating highlights has presented which pre-processes extracted Video Frames. Convolutional Neural Networks are then used to evaluate these highlighted frames. The proposed technique extracts and calculates characteristics utilized to generate summary movies. For training deep neural networks, cricket datasets have been used. Experimental results show that the proposed solution attains improved results than other advanced summarization methodologies. Experimental results show that the proposed video summarization method consistently generates high-quality reviews for all types of videos. The proposed video summarization method is easy to use, and it can also help extract highlights of cricket games with high accuracy.
近年来,视频监控技术已经在我们生活的方方面面变得无处不在。自动视频监控会产生大量的数据,在某些时候需要人工检查。主要的重点是在不牺牲真实信息的情况下,通过压缩或消除多余的帧来减少存储使用。目前的努力旨在缩小实际数据量与数据量之间日益扩大的差距。在大型视频集合中搜索关键事件既耗时又乏味。本文介绍了基于视频摘要的智能监控技术在各种应用中的应用。提出了一种通过预处理提取视频帧来生成高光的方法。然后使用卷积神经网络来评估这些突出显示的帧。该技术提取并计算用于生成摘要电影的特征。为了训练深度神经网络,已经使用了板球数据集。实验结果表明,与其他先进的摘要方法相比,该方法取得了更好的效果。实验结果表明,所提出的视频摘要方法对所有类型的视频都能产生高质量的评论。所提出的视频摘要方法易于使用,并且能够以较高的准确率提取板球比赛的亮点。
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引用次数: 0
Classification of Hot Jupiter Population through Statistical Framework 基于统计框架的热木星人口分类
Pub Date : 2022-05-01 DOI: 10.3329/jsr.v14i2.56497
S. Mondal
The recent trend in the discovery of a range of exoplanets opens up a door to evaluate their origin and classification under the light of different planetary attributes. This paper enthusiastically focused on a typical branch of exoplanets, hot Jupiter, and several planetary characteristics were observed to frame the population into substantive categories. In this paper, a statistical framework was also established to understand different planetary formation processes for hot Jupiters. Finally, the relevance of hot Jupiters in search of habitable planets is also discussed briefly.
最近一系列系外行星的发现趋势为根据不同的行星属性来评估它们的起源和分类打开了一扇门。这篇论文热情地关注了系外行星的一个典型分支——热木星,并观察了几个行星的特征,将其划分为实质性的类别。本文还建立了一个统计框架,以了解热木星的不同行星形成过程。最后,还简要讨论了热木星与寻找可居住行星的相关性。
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引用次数: 0
Metronidazole and 2-Methylimidazole as Corrosion Inhibitors in Microbiologically Influenced Corrosion 甲硝唑和2-甲基咪唑在微生物影响腐蚀中的缓蚀剂作用
Pub Date : 2022-05-01 DOI: 10.3329/jsr.v14i2.56113
J. Girase, P. Kamble, R. Dubey
Microbial corrosion behavior of mild steel was studied by exposing mild steel coupons in Barr’s medium inoculated with sulfate-reducing bacteria (Desulfovibrio desulphuricans). During the investigation, a considerable loss in weight and deterioration of microstructure of mild steel coupons surface was observed. Coupons were further exposed to culture media containing different concentrations of metronidazole (MNZ) and 2- methylimidazole (MIZ) inhibitors. The corrosion behavior of mild steel was measured by weight loss, electrochemical studies, and SEM. Polarization studies indicated the mixed-type behavior of these inhibitors. MNZ and MIZ exhibited 82.23 and 78.30 % inhibition efficiencies, respectively, as revealed by polarization measurements. The results show that the inhibition efficiencies increased with inhibitor concentration. The surface analysis was performed by scanning electron microscopy (SEM).
通过将低碳钢试样置于接种了硫酸盐还原菌(Desulfovibrio desulphurans)的Barr培养基中,研究了低碳钢的微生物腐蚀行为。在研究过程中,观察到低碳钢片材表面的重量损失和组织恶化。将样品进一步暴露于含有不同浓度甲硝唑(MNZ)和2-甲基咪唑(MIZ)抑制剂的培养基中。通过失重、电化学研究和扫描电镜对低碳钢的腐蚀行为进行了测定。极化研究表明这些抑制剂的混合型行为。极化测量结果表明,MNZ和MIZ的缓蚀效率分别为82.23%和78.30%。结果表明,缓蚀剂浓度越大,缓蚀效率越高。表面分析采用扫描电镜(SEM)进行。
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引用次数: 0
Prevalence of anaemia in pregnant women among rural population of Kurhani block, Muzaffarpur district Bihar: A cross-sectional Primary Health Center based study 比哈尔邦Muzaffarpur区Kurhani街区农村人口孕妇贫血患病率:一项基于初级卫生中心的横断面研究
Pub Date : 2022-01-01 DOI: 10.37398/jsr.2022.660506
K. Abhishek, Md Zahid Ansari, R. Topno, Madhukar, D. R. Purakayastha, Hemant Mahajan, Rakesh Bihari Verma, S. Chaturvedi, Dharmendra Kumar, Vidya Nand Rabi Das, S. Sinha, K. Kunal, Chandra Shekhar Lal, K. Pandey
Anaemia is a major public problem which is a matter of great concern for developing countries. In India, about 63% of women of aged between15 to 49 years are anaemic. Anaemia has severe consequences on the growing child and pregnant women. In this study, pregnant women in the age range 18- 45 years who consulted the Kurhani PHC of Muzaffarpur district for regular antenatal check-up were recruited. The participants were grouped on the basis of age. The blood samples from the recruited subjects were collected and a complete blood profile was done. The median age of the pregnant women participants was 25 years. Among 345 participants, 95% of the participants were of the age group 18 - 30 years. A total of 156 (45%) pregnant women were found anaemic with 72 (20.03%) having mild anaemia, 76 (22.87%) with moderate anaemia and 8 (2.31%) were severely anaemic. The findings from this study reveal the status of anaemia among the pregnant women of the local population; which will be used to design further epidemiological studies, and will pave the ways to explore the various aspects of anaemia in pregnancy; thus contribute to fulfill the mandate of the setup of Model Rural Health Research Unit at Kurhani-Muzaffarpur, Bihar.
贫血是一个重大的公共问题,是发展中国家非常关注的问题。在印度,年龄在15岁至49岁之间的女性中约有63%患有贫血症。贫血对成长中的儿童和孕妇有严重后果。在这项研究中,招募了年龄在18- 45岁之间的孕妇,她们在Muzaffarpur区的Kurhani初级保健医院进行定期产前检查。参与者按年龄分组。收集了招募的受试者的血液样本,并完成了完整的血液分析。孕妇参与者的中位年龄为25岁。在345名参与者中,95%的参与者年龄在18 - 30岁之间。孕妇贫血156例(45%),其中轻度贫血72例(20.03%),中度贫血76例(22.87%),重度贫血8例(2.31%)。这项研究的结果揭示了当地人口中孕妇的贫血状况;这将用于设计进一步的流行病学研究,并将为探索妊娠贫血的各个方面铺平道路;从而有助于完成在比哈尔邦库尔哈尼-穆扎法尔布尔建立农村卫生示范研究所的任务。
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引用次数: 0
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