C. Ruston, D. Holtkamp, Peiyang Li, J. Koziel, Aaron Stephan, Tina Loesekann, M. Torremorell, D. Murray, Katie Wedel, Clayton Johnson, P. Zaabel, P. Sundberg
The application of ultraviolet-C (UVC) light is not well understood in the swine industry, and best practices for applying UVC technology effectively and safely are lacking. This paper aims to summarize swine industry best practices for using UVC safely and maintenance requirements created as a result of a UVC workshop organized by the Swine Health Information Center. By understanding basic UVC physics, mechanism of action, safety procedures, and general maintenance requirements, the swine industry will be able to use UVC technology safely and effectively for decontamination of surfaces on swine farms.
紫外- c (UVC)光在养猪业中的应用还不是很清楚，并且缺乏有效和安全应用UVC技术的最佳实践。本文旨在总结养猪业安全使用UVC的最佳实践以及由养猪健康信息中心组织的UVC研讨会所产生的维护要求。通过了解基本的UVC物理、作用机制、安全程序和一般维护要求，养猪业将能够安全有效地使用UVC技术对养猪场表面进行净化。
{"title":"Effectively using ultraviolet-C light for supply decontamination on swine farms","authors":"C. Ruston, D. Holtkamp, Peiyang Li, J. Koziel, Aaron Stephan, Tina Loesekann, M. Torremorell, D. Murray, Katie Wedel, Clayton Johnson, P. Zaabel, P. Sundberg","doi":"10.54846/jshap/1248","DOIUrl":"https://doi.org/10.54846/jshap/1248","url":null,"abstract":"The application of ultraviolet-C (UVC) light is not well understood in the swine industry, and best practices for applying UVC technology effectively and safely are lacking. This paper aims to summarize swine industry best practices for using UVC safely and maintenance requirements created as a result of a UVC workshop organized by the Swine Health Information Center. By understanding basic UVC physics, mechanism of action, safety procedures, and general maintenance requirements, the swine industry will be able to use UVC technology safely and effectively for decontamination of surfaces on swine farms.","PeriodicalId":17095,"journal":{"name":"Journal of Swine Health and Production","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2022-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43898234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Alexandra K. Ford, R. Palinski, B. Lubbers, L. Tokach, A. G. Cino-Ozuna
We document a case series of abortions and placentitis in domestic pigs from the Midwest United States where aerobic bacterial cultures consistently isolated Trueperella abortisuis. Cases were submitted between 2017-2020 to the Kansas State Veterinary Diagnostic Lab. Microscopically, there was suppurative placentitis with necrosis and intralesional, gram-positive coccobacilli. In all cases, molecular diagnostics were negative for major causes of abortion in pigs. This is the first known report of T abortisuis isolated from swine abortions or placentitis in the United States.
{"title":"Placentitis and abortion in domestic pigs (Sus scrofa domesticus) associated with Trueperella abortisuis on US swine farms","authors":"Alexandra K. Ford, R. Palinski, B. Lubbers, L. Tokach, A. G. Cino-Ozuna","doi":"10.54846/jshap/1258","DOIUrl":"https://doi.org/10.54846/jshap/1258","url":null,"abstract":"We document a case series of abortions and placentitis in domestic pigs from the Midwest United States where aerobic bacterial cultures consistently isolated Trueperella abortisuis. Cases were submitted between 2017-2020 to the Kansas State Veterinary Diagnostic Lab. Microscopically, there was suppurative placentitis with necrosis and intralesional, gram-positive coccobacilli. In all cases, molecular diagnostics were negative for major causes of abortion in pigs. This is the first known report of T abortisuis isolated from swine abortions or placentitis in the United States.","PeriodicalId":17095,"journal":{"name":"Journal of Swine Health and Production","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43903826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
R. Hinson, K. McCormick, R. Moser, M. Ackerman, R. Main, J. Mahoney
Objective: To evaluate performance and physiological vitamin status of sows and progeny fed 2 vitamin supplementation levels, industry vs reduced (all vitamins reduced with fat-soluble vitamins added at National Research Council recommendations). Materials and methods: Sows (n = 244) were allotted in a randomized complete block design to 1 of 2 vitamin supplementation levels. At weaning, 765 progeny from a subset of sows were allotted to treatments in a 2 × 2 factorial arrangement of two sow and two nursery vitamin supplementation levels with 15 pens/treatment. Performance and vitamin status of sows and progeny were measured from farrowing to nursery exit. Results: Reduced vitamin supplementation reduced sow lactation feed intake (P = .01), hepatic vitamin A (P = .001), and serum vitamin D (P < .001), but did not affect sow body weight or litter performance. Regardless of vitamin levels fed to the sow, progeny fed reduced levels post weaning had decreased circulating (P < .001) and stored (P = .03) vitamin levels and a reduction in average daily gain (P < .001), average daily feed intake (P < .001), gain:feed ratio (P = .002), and body weight (P < .001) at the end of the nursery period compared to progeny fed industry levels. Implications: Reduced vitamin supplementation reduced sow feed intake without affecting sow or litter performance, but decreased circulating and stored vitamin levels in sows could impact long-term reproductive performance. Reduced vitamin inclusion levels in nursery diets reduced performance and serum vitamin concentrations compared to industry vitamin levels.
{"title":"Reduced vitamin supplementation with fat-soluble vitamins A, D, and E added at National Research Council requirements may not be adequate for optimal sow and progeny performance","authors":"R. Hinson, K. McCormick, R. Moser, M. Ackerman, R. Main, J. Mahoney","doi":"10.54846/jshap/1259","DOIUrl":"https://doi.org/10.54846/jshap/1259","url":null,"abstract":"Objective: To evaluate performance and physiological vitamin status of sows and progeny fed 2 vitamin supplementation levels, industry vs reduced (all vitamins reduced with fat-soluble vitamins added at National Research Council recommendations). Materials and methods: Sows (n = 244) were allotted in a randomized complete block design to 1 of 2 vitamin supplementation levels. At weaning, 765 progeny from a subset of sows were allotted to treatments in a 2 × 2 factorial arrangement of two sow and two nursery vitamin supplementation levels with 15 pens/treatment. Performance and vitamin status of sows and progeny were measured from farrowing to nursery exit. Results: Reduced vitamin supplementation reduced sow lactation feed intake (P = .01), hepatic vitamin A (P = .001), and serum vitamin D (P < .001), but did not affect sow body weight or litter performance. Regardless of vitamin levels fed to the sow, progeny fed reduced levels post weaning had decreased circulating (P < .001) and stored (P = .03) vitamin levels and a reduction in average daily gain (P < .001), average daily feed intake (P < .001), gain:feed ratio (P = .002), and body weight (P < .001) at the end of the nursery period compared to progeny fed industry levels. Implications: Reduced vitamin supplementation reduced sow feed intake without affecting sow or litter performance, but decreased circulating and stored vitamin levels in sows could impact long-term reproductive performance. Reduced vitamin inclusion levels in nursery diets reduced performance and serum vitamin concentrations compared to industry vitamin levels.","PeriodicalId":17095,"journal":{"name":"Journal of Swine Health and Production","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46207608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Lifetime performance and longevity are very important parameters of profitability in sow breeding. Opportunity to improve lifetime performance and longevity may be found in the rearing period and preparation of gilts for their future reproductive role. With the aim to prevent premature culling, it is possible to influence body condition, limb condition, mammary gland development, and proper function of the reproductive tract through nutrition, technology, and rearing strategies. Nutrition plays a very important role, as it can affect all the basic requirements for achieving satisfactory gilt performance. Selecting the most effective rearing strategy can be difficult because there are many factors affecting performance and longevity. The aim of this literature review is to provide up-to-date information on how sow longevity and performance can be influenced through choice of gilt rearing strategies and the important area of nutrition.
{"title":"Gilt rearing impacts on sow performance and longevity – a review","authors":"J. Belkova, M. Rozkot","doi":"10.54846/jshap/1254","DOIUrl":"https://doi.org/10.54846/jshap/1254","url":null,"abstract":"Lifetime performance and longevity are very important parameters of profitability in sow breeding. Opportunity to improve lifetime performance and longevity may be found in the rearing period and preparation of gilts for their future reproductive role. With the aim to prevent premature culling, it is possible to influence body condition, limb condition, mammary gland development, and proper function of the reproductive tract through nutrition, technology, and rearing strategies. Nutrition plays a very important role, as it can affect all the basic requirements for achieving satisfactory gilt performance. Selecting the most effective rearing strategy can be difficult because there are many factors affecting performance and longevity. The aim of this literature review is to provide up-to-date information on how sow longevity and performance can be influenced through choice of gilt rearing strategies and the important area of nutrition.","PeriodicalId":17095,"journal":{"name":"Journal of Swine Health and Production","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2022-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45286013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
B. Pepin, Todd E Williams, Carissa A. Odland, T. Spronk, J. Nerem
Carbon dioxide (CO2) is an American Veterinary Medical Association approved swine euthanasia method. A limitation for the use of CO2 during depopulation events, such as a foreign animal disease diagnosis, is the ability to apply the method to finisher and adult pigs. The common euthanasia methods in finishing and adult swine require individual animal handling and restraint, increasing human safety risk in large-scale depopulation events. This project validates the modification of a standard dump trailer into a mobile CO2 depopulation unit. The success of the method was verified with subdermal implants for monitoring heart rate, activity, and body temperature.
{"title":"Modification of a standard dump trailer into a mobile carbon dioxide depopulation unit for swine","authors":"B. Pepin, Todd E Williams, Carissa A. Odland, T. Spronk, J. Nerem","doi":"10.54846/jshap/1243","DOIUrl":"https://doi.org/10.54846/jshap/1243","url":null,"abstract":"Carbon dioxide (CO2) is an American Veterinary Medical Association approved swine euthanasia method. A limitation for the use of CO2 during depopulation events, such as a foreign animal disease diagnosis, is the ability to apply the method to finisher and adult pigs. The common euthanasia methods in finishing and adult swine require individual animal handling and restraint, increasing human safety risk in large-scale depopulation events. This project validates the modification of a standard dump trailer into a mobile CO2 depopulation unit. The success of the method was verified with subdermal implants for monitoring heart rate, activity, and body temperature.","PeriodicalId":17095,"journal":{"name":"Journal of Swine Health and Production","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2022-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43928164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This case study describes sudden deaths of pigs in a North Carolina production system at three different finishing facilities between June 2019 and February 2020. The cases involved 3- to 6-month-old pigs of the same genetics but from different sow farm flows. Pigs at all three sites had gross lesions that included firm, deep red to purple lungs. Based on laboratory results from tissue samples, all cases were diagnosed with Actinobacillus suis. Treatments and the significance of this disease in swine are discussed.
{"title":"Three cases of Actinobacillus suis in eastern North Carolina","authors":"E. Mahan-Riggs","doi":"10.54846/jshap/1239","DOIUrl":"https://doi.org/10.54846/jshap/1239","url":null,"abstract":"This case study describes sudden deaths of pigs in a North Carolina production system at three different finishing facilities between June 2019 and February 2020. The cases involved 3- to 6-month-old pigs of the same genetics but from different sow farm flows. Pigs at all three sites had gross lesions that included firm, deep red to purple lungs. Based on laboratory results from tissue samples, all cases were diagnosed with Actinobacillus suis. Treatments and the significance of this disease in swine are discussed.","PeriodicalId":17095,"journal":{"name":"Journal of Swine Health and Production","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2022-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41468600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Dr C. Grace Elijah, Manhattan KS Denison Ave, C. Grace, O. Harrison, Allison K. Blomme, PhD Jason C. Woodworth, PhD Cassandra K. Jones, PhD Chad B. Paulk, Dvm Jordan T. Gebhardt
Two feed mills and three breed-to-wean facilities were investigated after being diagnosed with porcine deltacoronavirus (PDCoV) with initial suspicion that feed manufacture and delivery processes were involved in disease transmission. Both feed mills were audited, and environmental samples collected in areas that were deemed high risk for virus contamination. All breed-to-wean facilities had PDCoV detected as would be expected, while the only positive samples for enteric coronaviruses associated with feed mills were feed delivery trucks. These results indicate that feed delivery surfaces can help spread virus during an ongoing disease outbreak and must be considered when determining the outbreak origin.
{"title":"Understanding the role of feed manufacturing and delivery within a series of porcine deltacoronavirus investigations","authors":"Dr C. Grace Elijah, Manhattan KS Denison Ave, C. Grace, O. Harrison, Allison K. Blomme, PhD Jason C. Woodworth, PhD Cassandra K. Jones, PhD Chad B. Paulk, Dvm Jordan T. Gebhardt","doi":"10.54846/jshap/1250","DOIUrl":"https://doi.org/10.54846/jshap/1250","url":null,"abstract":"Two feed mills and three breed-to-wean facilities were investigated after being diagnosed with porcine deltacoronavirus (PDCoV) with initial suspicion that feed manufacture and delivery processes were involved in disease transmission. Both feed mills were audited, and environmental samples collected in areas that were deemed high risk for virus contamination. All breed-to-wean facilities had PDCoV detected as would be expected, while the only positive samples for enteric coronaviruses associated with feed mills were feed delivery trucks. These results indicate that feed delivery surfaces can help spread virus during an ongoing disease outbreak and must be considered when determining the outbreak origin.","PeriodicalId":17095,"journal":{"name":"Journal of Swine Health and Production","volume":"1 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2022-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70951904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
B. Pepin, Carissa A. Odland, T. Spronk, Roy Elder, Todd E Williams
Objectives: This study aimed to evaluate the physiological and behavioral responses of pigs administered sodium nitrite, determine an ideal dosing rate by oral drenching of sodium nitrite for depopulation events, and evaluate the nitrite residue present in the ocular fluid and skeletal muscle after sodium nitrite administration. Materials and methods: Four groups of 10 market weight pigs (40 market weight pigs total) and 1 group of 10 sows were used. Each group of market weight animals received a different oral drench dose of sodium nitrite solution (1× [400-441 mg/kg], 2× [800-882 mg/kg], 2.5× [1000-1102 mg/kg], and 3× [1200-1323 mg/kg]) and was observed for distress behaviors. Two market weight animals in each treatment group were implanted with a monitor to measure body temperature, heart rate, and activity levels. The dosing rate with apparent best behavioral and physiological response was applied to the 10 sows and the same behaviors monitored. After death was confirmed, ocular fluid and skeletal muscle samples were collected from the sows. Results: An increased dosage of sodium nitrite greatly reduced the time to distress with a significant linear relationship. A higher frequency of vocalizations and the most frequent spikes in activity levels were observed in the lowest dosing group. No correlation was found between ocular fluid nitrite and skeletal muscle sodium nitrite concentrations. Implications: Oral drenching of sodium nitrite is a viable method for swine depopulation events. Higher doses of sodium nitrite have better welfare associations. Ocular fluid nitrite anion concentrations do not correlate with sodium nitrite skeletal muscle concentrations.
{"title":"Swine behavioral and physiological response to increasing sodium nitrite oral drench administration and resulting tissue residues","authors":"B. Pepin, Carissa A. Odland, T. Spronk, Roy Elder, Todd E Williams","doi":"10.54846/jshap/1241","DOIUrl":"https://doi.org/10.54846/jshap/1241","url":null,"abstract":"Objectives: This study aimed to evaluate the physiological and behavioral responses of pigs administered sodium nitrite, determine an ideal dosing rate by oral drenching of sodium nitrite for depopulation events, and evaluate the nitrite residue present in the ocular fluid and skeletal muscle after sodium nitrite administration. Materials and methods: Four groups of 10 market weight pigs (40 market weight pigs total) and 1 group of 10 sows were used. Each group of market weight animals received a different oral drench dose of sodium nitrite solution (1× [400-441 mg/kg], 2× [800-882 mg/kg], 2.5× [1000-1102 mg/kg], and 3× [1200-1323 mg/kg]) and was observed for distress behaviors. Two market weight animals in each treatment group were implanted with a monitor to measure body temperature, heart rate, and activity levels. The dosing rate with apparent best behavioral and physiological response was applied to the 10 sows and the same behaviors monitored. After death was confirmed, ocular fluid and skeletal muscle samples were collected from the sows. Results: An increased dosage of sodium nitrite greatly reduced the time to distress with a significant linear relationship. A higher frequency of vocalizations and the most frequent spikes in activity levels were observed in the lowest dosing group. No correlation was found between ocular fluid nitrite and skeletal muscle sodium nitrite concentrations. Implications: Oral drenching of sodium nitrite is a viable method for swine depopulation events. Higher doses of sodium nitrite have better welfare associations. Ocular fluid nitrite anion concentrations do not correlate with sodium nitrite skeletal muscle concentrations.","PeriodicalId":17095,"journal":{"name":"Journal of Swine Health and Production","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2021-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43511838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. O’Connor, S. Totton, C. Winder, D. Holtkamp, Gustavo Silva, J. Sargeant
A checklist for guiding authors in comprehensive reporting of swine individually or cluster-randomized controlled trials for journal abstracts or conference proceedings is shown. It is recommended that authors, conference organizers, and journal editors adopt this guideline to enhance study interpretation and use and reduce research wastage.
{"title":"TRaiTS: Template for Reporting of Trials in Short format - swine examples","authors":"A. O’Connor, S. Totton, C. Winder, D. Holtkamp, Gustavo Silva, J. Sargeant","doi":"10.54846/jshap/1247","DOIUrl":"https://doi.org/10.54846/jshap/1247","url":null,"abstract":"A checklist for guiding authors in comprehensive reporting of swine individually or cluster-randomized controlled trials for journal abstracts or conference proceedings is shown. It is recommended that authors, conference organizers, and journal editors adopt this guideline to enhance study interpretation and use and reduce research wastage.","PeriodicalId":17095,"journal":{"name":"Journal of Swine Health and Production","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2021-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44628915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Branko Angjelovski, B. Atanasov, Miroslav Kjosevski
Objective: Identify the occurrence of certain virulence genes of Escherichia coli vaginal isolates associated with postpartum dysgalactia syndrome (PDS) in sows. Materials and methods: Two hundred and two sows from 5 Macedonian pig farms were clinically examined for PDS 12 to 24 hours after farrowing. Vaginal swabs for bacteriological testing were taken from PDS-affected (PDSA, n = 47) and PDS-unaffected (PDSU, n = 155) sows. In total, 74 isolates of E coli were tested by multiplex polymerase chain reaction for the presence of virulence genes related to specific pathogenic strains. Results: Genes associated with extra-intestinal pathogenic E coli (ExPEC) strains were the most prevalent among all tested E coli isolates. The most dominant gene among all E coli isolates was fimC. The iss gene was more prevalent in PDSA sows compared to PDSU sows (P = .02). Multivariable logistic regression showed that lower parity sows (P ≤ .001) and presence of the iss (P = .003) and astA genes (P = .03) were correlated with the occurrence of PDS. Implications: Lower parity sows vaginally infected with E coli associated with particular ExPEC strains are at higher risk of developing PDS. Positive vaginal swabs for E coli and iss gene found early after farrowing were associated with PDS in sows. Classification of E coli into specific ExPEC pathotype was not possible by virulence genotyping only.
目的:确定与母猪产后泌乳不良综合征(PDS)相关的大肠杆菌阴道分离株的某些毒力基因的发生情况。材料与方法:对5个马其顿猪场的122头母猪进行产后12 ~ 24小时PDS临床检查。从感染pds (PDSA, n = 47)和未感染pds (PDSU, n = 155)的母猪身上取阴道拭子进行细菌学检测。采用多重聚合酶链反应法检测74株大肠杆菌是否存在与特定致病菌株相关的毒力基因。结果:与肠外致病性大肠杆菌(ExPEC)菌株相关的基因在所有检测的大肠杆菌分离株中最为普遍。所有大肠杆菌分离株中最显性基因为fimC。与PDSU母猪相比，iss基因在PDSA母猪中更为普遍(P = 0.02)。多变量logistic回归分析显示，低胎次母猪(P≤0.001)、iss基因(P = 0.003)和astA基因(P = 0.03)的存在与PDS的发生相关。结论:低胎次的母猪阴道感染与特定的大肠杆菌菌株相关的大肠杆菌，发生PDS的风险更高。分娩后早期阴道棉签中大肠杆菌和iss基因阳性与母猪PDS有关。仅通过毒力基因分型无法将大肠杆菌分类为特定的expc致病型。
{"title":"Virulence genes of Escherichia coli vaginal isolates associated with postpartum dysgalactia syndrome in sows","authors":"Branko Angjelovski, B. Atanasov, Miroslav Kjosevski","doi":"10.54846/jshap/1236","DOIUrl":"https://doi.org/10.54846/jshap/1236","url":null,"abstract":"Objective: Identify the occurrence of certain virulence genes of Escherichia coli vaginal isolates associated with postpartum dysgalactia syndrome (PDS) in sows. Materials and methods: Two hundred and two sows from 5 Macedonian pig farms were clinically examined for PDS 12 to 24 hours after farrowing. Vaginal swabs for bacteriological testing were taken from PDS-affected (PDSA, n = 47) and PDS-unaffected (PDSU, n = 155) sows. In total, 74 isolates of E coli were tested by multiplex polymerase chain reaction for the presence of virulence genes related to specific pathogenic strains. Results: Genes associated with extra-intestinal pathogenic E coli (ExPEC) strains were the most prevalent among all tested E coli isolates. The most dominant gene among all E coli isolates was fimC. The iss gene was more prevalent in PDSA sows compared to PDSU sows (P = .02). Multivariable logistic regression showed that lower parity sows (P ≤ .001) and presence of the iss (P = .003) and astA genes (P = .03) were correlated with the occurrence of PDS. Implications: Lower parity sows vaginally infected with E coli associated with particular ExPEC strains are at higher risk of developing PDS. Positive vaginal swabs for E coli and iss gene found early after farrowing were associated with PDS in sows. Classification of E coli into specific ExPEC pathotype was not possible by virulence genotyping only.","PeriodicalId":17095,"journal":{"name":"Journal of Swine Health and Production","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2021-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48216667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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