A newly populated sow herd suffered an outbreak of splayleg and congenital tremors in the offspring. Some piglets were affected by one or the other condition, others by both. The problem lasted for about 9 months and was associated with significant losses, mainly because of the splayleg component. Most piglets with only congenital tremors were able to survive and their condition improved as they got older. Piglets with congenital tremors had histological lesions consistent with this condition, and pestivirus K (formerly atypical porcine pestivirus) was identified from their nervous tissues.
{"title":"An outbreak of splayleg and congenital tremors in piglets farrowed by a newly populated sow herd","authors":"R. Desrosiers, Elisabeth Carriere, A. Broes","doi":"10.54846/jshap/1275","DOIUrl":"https://doi.org/10.54846/jshap/1275","url":null,"abstract":"A newly populated sow herd suffered an outbreak of splayleg and congenital tremors in the offspring. Some piglets were affected by one or the other condition, others by both. The problem lasted for about 9 months and was associated with significant losses, mainly because of the splayleg component. Most piglets with only congenital tremors were able to survive and their condition improved as they got older. Piglets with congenital tremors had histological lesions consistent with this condition, and pestivirus K (formerly atypical porcine pestivirus) was identified from their nervous tissues.","PeriodicalId":17095,"journal":{"name":"Journal of Swine Health and Production","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42302385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Monteiro, A. Poor, B. Muro, R. Carnevale, D. Leal, C. Garbossa, Andrea Moreno, G. Almond
The development of new generation sequencing methods and the reduction in the cost per base sequenced over the past few years is drawing the attention of the pig industry to microbiome understanding and modulation. In recent years, there has been an increase in the number of articles published related to microbiome studies in swine. With respect to sows, microbiome studies mainly focused on the gut, with some studies evaluating the reproductive tract and mammary microbiome. However, studies about urinary microbiome are still lacking. The present literature indicates that the microbiome in the sow’s gut can affect the microbiome in other body parts. Moreover, the understanding of the dynamics and interactions among microbial populations within the sow or the herd has led to improvements in animal health and reproductive performance. This review provides new insights related to sow intestinal, urinary, mammary, and reproductive microbiomes and their relationships with reproductive outcomes, diseases, and early colonization in offspring by gathering the most recent work in this field as well as pinpoints information gaps that require further investigation. This literature review also sheds light on the knowledge regarding the role of microbiomes in the reduction of antimicrobial use.
{"title":"The sow microbiome: Current and future perspectives to maximize the productivity in swine herds","authors":"M. Monteiro, A. Poor, B. Muro, R. Carnevale, D. Leal, C. Garbossa, Andrea Moreno, G. Almond","doi":"10.54846/jshap/1277","DOIUrl":"https://doi.org/10.54846/jshap/1277","url":null,"abstract":"The development of new generation sequencing methods and the reduction in the cost per base sequenced over the past few years is drawing the attention of the pig industry to microbiome understanding and modulation. In recent years, there has been an increase in the number of articles published related to microbiome studies in swine. With respect to sows, microbiome studies mainly focused on the gut, with some studies evaluating the reproductive tract and mammary microbiome. However, studies about urinary microbiome are still lacking. The present literature indicates that the microbiome in the sow’s gut can affect the microbiome in other body parts. Moreover, the understanding of the dynamics and interactions among microbial populations within the sow or the herd has led to improvements in animal health and reproductive performance. This review provides new insights related to sow intestinal, urinary, mammary, and reproductive microbiomes and their relationships with reproductive outcomes, diseases, and early colonization in offspring by gathering the most recent work in this field as well as pinpoints information gaps that require further investigation. This literature review also sheds light on the knowledge regarding the role of microbiomes in the reduction of antimicrobial use.","PeriodicalId":17095,"journal":{"name":"Journal of Swine Health and Production","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45090686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
K. Vonnahme, M. Vasquez-Hidalgo, J. Angulo, D. Amodie, M. Mellencamp, Lucina Galina Pantoja
Objective: To determine viremia, percentage lung lesions, average daily gain (ADG), and their associations after a porcine reproductive and respiratory syndrome virus-2 (PRRSV-2) lineage 1 (open reading frame 5 restriction fragment length polymorphism 1-7-4 [ORF5 RFLP 1-7-4]) challenge in pigs vaccinated with either a PRRSV-2 lineage 8 modified live virus (MLV) vaccine, a PRRSV-2 lineage 1 MLV vaccine, or not vaccinated. Materials and methods: Pigs were vaccinated with either Fostera PRRS (n = 52), Prevacent PRRS (n = 50), or sterile water (nonvaccinated; n = 47). Four weeks after vaccination, all animals were challenged with PRRSV-2 lineage 1 ORF5 RFLP 1-7-4. Viremia was determined at 3-, 6-, and 12-days post challenge. Body weights were recorded to determine ADG throughout the experiment. Percentage lung lesions were assessed on day 40 (12 days post challenge). Results: Vaccination with either vaccine reduced (P < .001) lung lesions, increased (P < .001) ADG post challenge, and better controlled viremia (P < .001) compared to nonvaccinated pigs. Implication: A commercially available PRRSV-2 lineage 8 vaccine was as effective as a PRRSV-2 lineage 1 vaccine against a heterologous PRRSV-2 lineage 1 viral challenge.
{"title":"Viral load, lung lesions, and average daily gain in a porcine reproductive and respiratory syndrome virus-2 challenge model","authors":"K. Vonnahme, M. Vasquez-Hidalgo, J. Angulo, D. Amodie, M. Mellencamp, Lucina Galina Pantoja","doi":"10.54846/jshap/1257","DOIUrl":"https://doi.org/10.54846/jshap/1257","url":null,"abstract":"Objective: To determine viremia, percentage lung lesions, average daily gain (ADG), and their associations after a porcine reproductive and respiratory syndrome virus-2 (PRRSV-2) lineage 1 (open reading frame 5 restriction fragment length polymorphism 1-7-4 [ORF5 RFLP 1-7-4]) challenge in pigs vaccinated with either a PRRSV-2 lineage 8 modified live virus (MLV) vaccine, a PRRSV-2 lineage 1 MLV vaccine, or not vaccinated. Materials and methods: Pigs were vaccinated with either Fostera PRRS (n = 52), Prevacent PRRS (n = 50), or sterile water (nonvaccinated; n = 47). Four weeks after vaccination, all animals were challenged with PRRSV-2 lineage 1 ORF5 RFLP 1-7-4. Viremia was determined at 3-, 6-, and 12-days post challenge. Body weights were recorded to determine ADG throughout the experiment. Percentage lung lesions were assessed on day 40 (12 days post challenge). Results: Vaccination with either vaccine reduced (P < .001) lung lesions, increased (P < .001) ADG post challenge, and better controlled viremia (P < .001) compared to nonvaccinated pigs. Implication: A commercially available PRRSV-2 lineage 8 vaccine was as effective as a PRRSV-2 lineage 1 vaccine against a heterologous PRRSV-2 lineage 1 viral challenge.","PeriodicalId":17095,"journal":{"name":"Journal of Swine Health and Production","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46622398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective: To determine the impact of lameness on sow productivity and longevity and evaluate the effects of housing management on the removal of lame sows in herds using pen gestation. Materials and methods: Retrospective production records and information on housing methods were collected from 23 farms using pen gestation and analyzed for the removal of 214,254 sows from 2014 through 2020. Statistical analyses were performed to evaluate differences in longevity, productivity, and the impact of housing methods. Results: Lameness was the third most reported cause of removal for sows in the study (13.7%). Sows culled for lameness spent significantly fewer days in the herd (P < .001), resulting in fewer litters (P < .001). The odds of removal for lameness were increased by several farm level factors including using dynamic groups and decreasing square footage (P < .05). Implications: Lameness is one of the top 3 reasons reported for sow removal and those sows are costly as they leave the herd earlier, are less productive, and are more likely to die or be euthanized versus culled. Housing methods play a role in the odds of removal for lameness and should be further investigated.
{"title":"Effects of lameness on productivity and longevity for sows in pen","authors":"A. Hallowell, M. Pierdon","doi":"10.54846/jshap/1271","DOIUrl":"https://doi.org/10.54846/jshap/1271","url":null,"abstract":"Objective: To determine the impact of lameness on sow productivity and longevity and evaluate the effects of housing management on the removal of lame sows in herds using pen gestation. Materials and methods: Retrospective production records and information on housing methods were collected from 23 farms using pen gestation and analyzed for the removal of 214,254 sows from 2014 through 2020. Statistical analyses were performed to evaluate differences in longevity, productivity, and the impact of housing methods. Results: Lameness was the third most reported cause of removal for sows in the study (13.7%). Sows culled for lameness spent significantly fewer days in the herd (P < .001), resulting in fewer litters (P < .001). The odds of removal for lameness were increased by several farm level factors including using dynamic groups and decreasing square footage (P < .05). Implications: Lameness is one of the top 3 reasons reported for sow removal and those sows are costly as they leave the herd earlier, are less productive, and are more likely to die or be euthanized versus culled. Housing methods play a role in the odds of removal for lameness and should be further investigated.","PeriodicalId":17095,"journal":{"name":"Journal of Swine Health and Production","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43769746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Estienne, K. Williams, N. Emami, S. Clark-Deener, R. Dalloul
Objective: To determine the effects of iron treatments on hematology, hepcidin, and growth in weaned pigs fed copper-supplemented diets. Materials and methods: Pigs were allocated to a 3 × 2 factorial arrangement of treatments (4 pens/treatment combination, 3 pigs/pen) with factors being intramuscular iron (200 mg at birth; 100 mg at birth and weaning [22.4 days of age]; or 100 mg at birth and 14 days of age) and dietary copper (14 [control] or 250 ppm [supplemented]). Blood was sampled at days 0, 7, and 49 post weaning. Results: Pigs receiving 100 mg iron at birth and weaning, but not pigs in the other groups, had hemoglobin concentrations consistent with iron deficiency at day 0 (iron treatment × day, P < .001). For pigs receiving 100 mg iron at birth and 14 days of age, hepcidin concentrations were greater in control pigs than copper-supplemented pigs (iron treatment × diet, P = .06). A diet × day interaction (P = .07) existed for hepcidin, with concentrations greater in control vs copper-supplemented pigs on day 49. Pigs receiving iron at day 14 of age had the greatest (P = .01) weaning weights. Gain from day 0 to 7 was enhanced (P = .03) by 250 ppm copper but nursery performance (day 0-49) was unaffected by iron treatment. Implications: Pigs receiving 100 mg iron at birth were iron deficient at weaning. Treatment with iron at 14 days of age could improve weaning weights and prevent iron deficiency at weaning. Age-related increases in hepcidin were decreased by additional copper supplementation.
{"title":"Effects of different parenteral iron treatment regimens on hematology characteristics, serum concentrations of hepcidin, and growth performance in pigs fed nursery diets supplemented with copper","authors":"M. Estienne, K. Williams, N. Emami, S. Clark-Deener, R. Dalloul","doi":"10.54846/jshap/1288","DOIUrl":"https://doi.org/10.54846/jshap/1288","url":null,"abstract":"Objective: To determine the effects of iron treatments on hematology, hepcidin, and growth in weaned pigs fed copper-supplemented diets. Materials and methods: Pigs were allocated to a 3 × 2 factorial arrangement of treatments (4 pens/treatment combination, 3 pigs/pen) with factors being intramuscular iron (200 mg at birth; 100 mg at birth and weaning [22.4 days of age]; or 100 mg at birth and 14 days of age) and dietary copper (14 [control] or 250 ppm [supplemented]). Blood was sampled at days 0, 7, and 49 post weaning. Results: Pigs receiving 100 mg iron at birth and weaning, but not pigs in the other groups, had hemoglobin concentrations consistent with iron deficiency at day 0 (iron treatment × day, P < .001). For pigs receiving 100 mg iron at birth and 14 days of age, hepcidin concentrations were greater in control pigs than copper-supplemented pigs (iron treatment × diet, P = .06). A diet × day interaction (P = .07) existed for hepcidin, with concentrations greater in control vs copper-supplemented pigs on day 49. Pigs receiving iron at day 14 of age had the greatest (P = .01) weaning weights. Gain from day 0 to 7 was enhanced (P = .03) by 250 ppm copper but nursery performance (day 0-49) was unaffected by iron treatment. Implications: Pigs receiving 100 mg iron at birth were iron deficient at weaning. Treatment with iron at 14 days of age could improve weaning weights and prevent iron deficiency at weaning. Age-related increases in hepcidin were decreased by additional copper supplementation.","PeriodicalId":17095,"journal":{"name":"Journal of Swine Health and Production","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45943403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Sweeney, Lacie A Gunnett, D. Mohan Kumar, B. Lunt, Lucina Galina Pantoja, D. Bade, Chandra M. Machin
Objective: To report the in vitro susceptibility to veterinary antimicrobial agents of Actinobacillus pleuropneumoniae, Bordetella bronchiseptica, Pasteurella multocida, and Streptococcus suis isolated from diseased pigs in the United States and Canada from 2016 to 2020. Materials and methods: In vitro broth microdilution susceptibility testing for minimal inhibitory concentration values were performed using ten antimicrobial agents (ampicillin, ceftiofur, danofloxacin, enrofloxacin, florfenicol, penicillin, tetracycline, tilmicosin, trimethoprim-sulfamethoxazole, and tulathromycin) with A pleuropneumoniae (n = 250), B bronchiseptica (n = 602), P multocida (n = 874), and S suis (n = 1223) following methods and susceptibility breakpoints approved by the Clinical and Laboratory Standards Institute. Results: Actinobacillus pleuropneumoniae isolates were 100% susceptible to ceftiofur, florfenicol, and tulathromycin and P multocida isolates were 100% susceptible to ceftiofur. High rates of susceptibility (95% to > 99%) were observed for A pleuropneumoniae to tilmicosin; for P multocida to ampicillin, enrofloxacin, florfenicol, penicillin, tilmicosin, and tulathromycin; for S suis to ampicillin and florfenicol; and for B bronchiseptica to tulathromycin. Tetracycline exhibited low susceptibility rates against A pleuropneumoniae (0% to 10.6%), P multocida (23.2% to 38.2%), and S suis (0.8% to 2.1%). No susceptibility of B bronchiseptica to ampicillin (0%) and low rates of susceptibility to florfenicol (3.9% to 15.2%) were also observed. Implications: Under the conditions of this study, the predominant pathogens associated with swine respiratory disease in the United States and Canada, A pleuropneumoniae, B bronchiseptica, P multocida, and S suis collected during 2016 to 2020, display high rates of susceptibility to most veterinary antimicrobial agents.
{"title":"Antimicrobial susceptibility of Actinobacillus pleuropneumoniae, Bordetella bronchiseptica, Pasteurella multocida, and Streptococcus suis isolated from diseased pigs in the United States and Canada, 2016 to 2020","authors":"M. Sweeney, Lacie A Gunnett, D. Mohan Kumar, B. Lunt, Lucina Galina Pantoja, D. Bade, Chandra M. Machin","doi":"10.54846/jshap/1282","DOIUrl":"https://doi.org/10.54846/jshap/1282","url":null,"abstract":"Objective: To report the in vitro susceptibility to veterinary antimicrobial agents of Actinobacillus pleuropneumoniae, Bordetella bronchiseptica, Pasteurella multocida, and Streptococcus suis isolated from diseased pigs in the United States and Canada from 2016 to 2020. Materials and methods: In vitro broth microdilution susceptibility testing for minimal inhibitory concentration values were performed using ten antimicrobial agents (ampicillin, ceftiofur, danofloxacin, enrofloxacin, florfenicol, penicillin, tetracycline, tilmicosin, trimethoprim-sulfamethoxazole, and tulathromycin) with A pleuropneumoniae (n = 250), B bronchiseptica (n = 602), P multocida (n = 874), and S suis (n = 1223) following methods and susceptibility breakpoints approved by the Clinical and Laboratory Standards Institute. Results: Actinobacillus pleuropneumoniae isolates were 100% susceptible to ceftiofur, florfenicol, and tulathromycin and P multocida isolates were 100% susceptible to ceftiofur. High rates of susceptibility (95% to > 99%) were observed for A pleuropneumoniae to tilmicosin; for P multocida to ampicillin, enrofloxacin, florfenicol, penicillin, tilmicosin, and tulathromycin; for S suis to ampicillin and florfenicol; and for B bronchiseptica to tulathromycin. Tetracycline exhibited low susceptibility rates against A pleuropneumoniae (0% to 10.6%), P multocida (23.2% to 38.2%), and S suis (0.8% to 2.1%). No susceptibility of B bronchiseptica to ampicillin (0%) and low rates of susceptibility to florfenicol (3.9% to 15.2%) were also observed. Implications: Under the conditions of this study, the predominant pathogens associated with swine respiratory disease in the United States and Canada, A pleuropneumoniae, B bronchiseptica, P multocida, and S suis collected during 2016 to 2020, display high rates of susceptibility to most veterinary antimicrobial agents.","PeriodicalId":17095,"journal":{"name":"Journal of Swine Health and Production","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2022-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45364579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Senecavirus A (SVA) has been demonstrated to be a causative agent for vesicular disease in swine. It is clinically indistinguishable from other agents that cause vesicular disease such as foot-and-mouth disease virus (FMDV), which is a reportable foreign animal disease (FAD). Thus, an investigation is initiated to rule out FMDV every time a vesicle is observed. Senecavirus A has now been reported across the Americas and Asia, and it appears the ecology of this virus has changed from sporadic infections to an endemic disease in some areas. In addition to vesicular disease, there have also been reports of increased neonatal mortality on affected sow farms. Knowledge about the pathogenesis of SVA in swine can provide many benefits to the swine industry. Understanding how long the virus can be detected in various sample types after infection can aide in choosing the correct samples to collect for diagnosis. In addition, the duration of virus shedding can help determine measures to control virus spread between animals. Prevention of SVA infection and disease with an efficacious vaccine could improve swine welfare, minimize SVA transmission, and reduce the burden of FAD investigations.
{"title":"Senecavirus A: Frequently asked questions","authors":"A. Buckley, K. Lager","doi":"10.54846/jshap/1270","DOIUrl":"https://doi.org/10.54846/jshap/1270","url":null,"abstract":"Senecavirus A (SVA) has been demonstrated to be a causative agent for vesicular disease in swine. It is clinically indistinguishable from other agents that cause vesicular disease such as foot-and-mouth disease virus (FMDV), which is a reportable foreign animal disease (FAD). Thus, an investigation is initiated to rule out FMDV every time a vesicle is observed. Senecavirus A has now been reported across the Americas and Asia, and it appears the ecology of this virus has changed from sporadic infections to an endemic disease in some areas. In addition to vesicular disease, there have also been reports of increased neonatal mortality on affected sow farms. Knowledge about the pathogenesis of SVA in swine can provide many benefits to the swine industry. Understanding how long the virus can be detected in various sample types after infection can aide in choosing the correct samples to collect for diagnosis. In addition, the duration of virus shedding can help determine measures to control virus spread between animals. Prevention of SVA infection and disease with an efficacious vaccine could improve swine welfare, minimize SVA transmission, and reduce the burden of FAD investigations.","PeriodicalId":17095,"journal":{"name":"Journal of Swine Health and Production","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2022-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47396914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
O. Osemeke, T. Donovan, Katelyn Dion, D. Holtkamp, D. Linhares
Using retrospective data from 6 breed-to-wean herds over 4 years, porcine reproductive and respiratory syndrome virus (PRRSV) statuses were assigned by week according to the 2021 American Association of Swine Veterinarians PRRSV classification. Productivity changes were characterized as herds transitioned through status categories. Overall, productivity improved as farm status improved.
{"title":"Characterization of changes in productivity parameters as breeding herds transitioned through the 2021 PRRSV breeding herd classification system","authors":"O. Osemeke, T. Donovan, Katelyn Dion, D. Holtkamp, D. Linhares","doi":"10.54846/jshap/1269","DOIUrl":"https://doi.org/10.54846/jshap/1269","url":null,"abstract":"Using retrospective data from 6 breed-to-wean herds over 4 years, porcine reproductive and respiratory syndrome virus (PRRSV) statuses were assigned by week according to the 2021 American Association of Swine Veterinarians PRRSV classification. Productivity changes were characterized as herds transitioned through status categories. Overall, productivity improved as farm status improved.","PeriodicalId":17095,"journal":{"name":"Journal of Swine Health and Production","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2022-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46132519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
R. Magtoto, B. Armenta-Leyva, P. Dizon-Magtoto, Ting-Yu Cheng, M. Clavijo, C. Johnson, W. Lopez, D. Baum, J. Zimmerman, L. Gimenez-Lirola
The diagnostic performance of a commercial Mycoplasma hyopneumoniae (MHP) serum enzyme-linked immunosorbent assay (ELISA) was evaluated for MHP antibody detection in processing fluids (n = 494) using samples from three commercial swine farms. Based on historical monitoring, one farm was considered MHP positive and two were considered MHP negative. Samples were tested at a 1:10 dilution and diagnostic sensitivities and specificities estimated for specific ELISA sample-to-positive (S:P) cutoffs. At S:P ≥ 0.40, diagnostic sensitivity and specificity were estimated as 97.6% and 100.0%, respectively. Overall, the results suggest that processing fluids can be used for MHP antibody surveillance in breeding herds.
{"title":"Performance of a Mycoplasma hyopneumoniae serum ELISA for antibody detection in processing fluids","authors":"R. Magtoto, B. Armenta-Leyva, P. Dizon-Magtoto, Ting-Yu Cheng, M. Clavijo, C. Johnson, W. Lopez, D. Baum, J. Zimmerman, L. Gimenez-Lirola","doi":"10.54846/jshap/1265","DOIUrl":"https://doi.org/10.54846/jshap/1265","url":null,"abstract":"The diagnostic performance of a commercial Mycoplasma hyopneumoniae (MHP) serum enzyme-linked immunosorbent assay (ELISA) was evaluated for MHP antibody detection in processing fluids (n = 494) using samples from three commercial swine farms. Based on historical monitoring, one farm was considered MHP positive and two were considered MHP negative. Samples were tested at a 1:10 dilution and diagnostic sensitivities and specificities estimated for specific ELISA sample-to-positive (S:P) cutoffs. At S:P ≥ 0.40, diagnostic sensitivity and specificity were estimated as 97.6% and 100.0%, respectively. Overall, the results suggest that processing fluids can be used for MHP antibody surveillance in breeding herds.","PeriodicalId":17095,"journal":{"name":"Journal of Swine Health and Production","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2022-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48292515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A 2500-sow herd previously free of, and unvaccinated for, Mycoplasma hyopneumoniae became infected. Both M hyopneumoniae and influenza A H1N1pdm09 virus were identified in sows showing clinical signs. Coughing lasting 2 to 4 days was observed in approximately 10% of sows and 26 sows died over the course of the outbreak. There was no apparent impact on performance indicators. Polymerase chain reaction and serological results showed that M hyopneumoniae progression within the herd was fast and that infection may have occurred within a few weeks. An elimination program was quickly implemented so that sale of negative animals could resume.
{"title":"Infection of a naïve sow herd with Mycoplasma hyopneumoniae","authors":"R. Desrosiers, Jacques Miclette, A. Broes","doi":"10.54846/jshap/1262","DOIUrl":"https://doi.org/10.54846/jshap/1262","url":null,"abstract":"A 2500-sow herd previously free of, and unvaccinated for, Mycoplasma hyopneumoniae became infected. Both M hyopneumoniae and influenza A H1N1pdm09 virus were identified in sows showing clinical signs. Coughing lasting 2 to 4 days was observed in approximately 10% of sows and 26 sows died over the course of the outbreak. There was no apparent impact on performance indicators. Polymerase chain reaction and serological results showed that M hyopneumoniae progression within the herd was fast and that infection may have occurred within a few weeks. An elimination program was quickly implemented so that sale of negative animals could resume.","PeriodicalId":17095,"journal":{"name":"Journal of Swine Health and Production","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2022-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43831657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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