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Physiological Responses of Adults during Soil-mixing Activities Based on the Presence of Soil Microorganisms: A Metabolomics Approach 基于土壤微生物存在的土壤混合活动中成虫生理反应:代谢组学方法
IF 1.9 4区 农林科学 Q3 HORTICULTURE Pub Date : 2022-05-01 DOI: 10.21273/jashs05146-21
Seon-Ok Kim, S. Son, Min Ji Kim, C. Lee, Sin-Ae Park
Mycobacterium vaccae is a species of nonpathogenic bacterium that lives naturally in soil. This study compared the physiological effects at a metabolomic level with autonomic nervous system responses in adults during soil-mixing activities, based on the presence or absence of M. vaccae in the soil. Twenty-nine adult participants performed soil-mixing activities for 5 minutes using sterilized soil with culture media and M. vaccae, respectively. Blood samples were drawn twice from each participant after each activity. Electroencephalograms and electrocardiograms were measured during the activity. Serum metabolites underwent metabolite profiling by gas chromatography, followed by multivariate analyses. Soil-emitted volatile organic compounds were identified using the solid-phase microextraction and gas chromatography-mass spectroscopy, followed by multivariate analyses. The volatile compound analysis revealed that the metabolites related to esters and sulfur-containing compounds are greater in soil with M. vaccae. Serum metabolomics revealed that the treatment group (soil inoculated by M. vaccae) possesses relatively higher levels of inter-alia organic and amino acids compared with the control group (soil mixed with culture media). In the treatment group, the electroencephalogram and electrocardiogram revealed that alpha band activity of the occipital lobe increases, while heart rate decreases. This study concludes that M. vaccae soil contact can affect human metabolic and autonomic reactions.
母牛分枝杆菌是一种自然生活在土壤中的非致病细菌。这项研究比较了在土壤混合活动中,基于土壤中是否存在M.vaccae,成年人在代谢组水平上的生理效应和自主神经系统反应。29名成年参与者分别使用无菌土壤和培养基和M.vaccae进行了5分钟的土壤混合活动。每个参与者在每次活动后抽取两次血样。在活动期间测量脑电图和心电图。血清代谢物通过气相色谱法进行代谢物分析,然后进行多变量分析。采用固相微萃取和气相色谱-质谱法,然后进行多元分析,对土壤排放的挥发性有机化合物进行了鉴定。挥发性化合物分析表明,土壤中与酯类和含硫化合物有关的代谢产物较多。血清代谢组学显示,与对照组(混合培养基的土壤)相比,处理组(接种M.vaccae的土壤)具有相对较高的有机酸和氨基酸水平。在治疗组中,脑电图和心电图显示枕叶的α带活动增加,而心率下降。本研究的结论是,与土壤接触会影响人类的代谢和自主反应。
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引用次数: 4
DNA Content Estimation in the Genus Salvia 丹参属植物DNA含量的测定
IF 1.9 4区 农林科学 Q3 HORTICULTURE Pub Date : 2022-05-01 DOI: 10.21273/jashs05175-21
R. Maynard, J. Ruter
Salvia is a genetically diverse genus in the Lamiaceae family, with hundreds of species distributed globally. With base chromosome numbers ranging from 6 to 19 and ploidy levels ranging from diploid to octoploid, the genus has been proposed to be subdivided based on molecular data rather than morphology. However, little is known about total DNA content across the genus. The DNA content of 141 Salvia genotypes were analyzed using flow cytometry. Samples of Salvia were stained with propidium iodide and compared with the internal standards Pisum sativum ‘Ctirad’ and Solanum lycopersicum ‘Stupické’ to generate estimations of DNA content. Holoploid 2C genome sizes of the analyzed Salvia ranged from 0.63 pg to 6.12 pg. DNA content showed a wide distribution across chromosome number, ploidy, and clade. The wide distribution of DNA content across the genus further indicates the diversity of Salvia and may be useful for future breeding efforts.
鼠尾草是鼠尾草科的一个遗传多样性属,在全球分布着数百种。基染色体数目从6到19不等,倍性水平从二倍体到八倍体不等,该属已被提出根据分子数据而不是形态学来细分。然而,对整个属的总DNA含量知之甚少。用流式细胞术分析了141个鼠尾草基因型的DNA含量。用碘化丙啶对丹参样品进行染色,并与内标Pisum sativum ' Ctirad '和Solanum lycopersicum ' stupick '进行比较,得出DNA含量的估计。所分析的鼠尾草全倍体2C基因组大小在0.63 ~ 6.12 pg之间,DNA含量在染色体数目、倍性和进化枝上分布广泛。DNA含量在整个属中的广泛分布进一步表明了鼠尾草的多样性,并可能对未来的育种工作有用。
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引用次数: 2
Proteomic Analysis of Pollen–Stigma Interaction between Vitis rotundifolia and Vitis vinifera 圆叶葡萄与葡萄花粉柱头互作的蛋白质组学分析
IF 1.9 4区 农林科学 Q3 HORTICULTURE Pub Date : 2022-05-01 DOI: 10.21273/jashs05153-21
Bo Wang, Wei-min Wu, Xi-cheng Wang, Zhuang-wei Wang, Y. Qian
Muscadine grape (Vitis rotundifolia) is highly resistant to many diseases and insects that attack european grape (Vitis vinifera). However, distant hybridization incompatibility between V. rotundifolia (female) and V. vinifera (male) impedes the utilization of V. rotundifolia in grape breeding. This study used fourth-dimension label-free protein quantitation to detect the key genes and pathways in the V. rotundifolia stigma after self-pollination (V. rotundifolia × V. rotundifolia) and cross-pollination (V. rotundifolia × V. vinifera). A histological analysis showed that pollen tube growth in the stigma of V. rotundifolia was arrested 8 hours after cross-pollination, but not after self-pollination. A proteomic analysis identified 32 differentially expressed proteins (DEPs) in the stigma of V. rotundifolia between self-pollination and cross-pollination. A heatmap analysis grouped these DEPs into four clusters. The top gene ontology terms were ATPase-coupled transmembrane transporter activity, extracellular region, DNA replication, and cellular carbohydrate biosynthetic process. A Kyoto Encyclopedia of Genes and Genomes analysis revealed that these DEPs participated in DNA replication and starch and sucrose metabolism pathways. The downregulated A5AY88, D7TJ35, D7SU26, F6HJI1, and F6GUE7 may have a role in cross incompatibility. This study revealed the cross incompatibility of grapes at histological and proteomic levels.
麝香葡萄(Vitis rotundifolia)对侵袭欧洲葡萄(Vitis vinifera)的许多疾病和昆虫具有很强的抵抗力。然而,雌性圆叶葡萄与雄性圆叶葡萄的远缘杂交不亲和性阻碍了圆叶葡萄在葡萄育种中的利用。本研究采用四维无标记蛋白定量技术,对自花授粉(V. rotundifolia × V. rotundifolia)和异花授粉(V. rotundifolia × V. vinifera)后柱头的关键基因和通路进行了检测。组织学分析表明,异花授粉后8小时柱头花粉管生长停止,自花授粉后无花粉管生长。通过蛋白质组学分析,鉴定了自花授粉和异花授粉过程中圆形叶柱头中32个差异表达蛋白(DEPs)。热图分析将这些dep分为四类。排在前几位的基因本体术语是atp酶偶联跨膜转运蛋白活性、细胞外区域、DNA复制和细胞碳水化合物生物合成过程。京都基因和基因组百科分析显示,这些dep参与DNA复制和淀粉和蔗糖代谢途径。下调的A5AY88、D7TJ35、D7SU26、F6HJI1和F6GUE7可能在交叉不亲和中起作用。本研究揭示了葡萄在组织学和蛋白质组学水平上的杂交不亲和性。
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引用次数: 0
Assessment of Prunus Rootstock Accessions Using Chloroplast and Nuclear Microsatellites 利用叶绿体和核微卫星评价李树砧木材料
IF 1.9 4区 农林科学 Q3 HORTICULTURE Pub Date : 2022-03-01 DOI: 10.21273/jashs05137-21
Chunxian Chen, L. Rodríguez-Bonilla, T. Beckman
A rootstock collection of Prunus species and hybrids is maintained at the U.S. Department of Agriculture stone fruit breeding program at Byron, GA. We genotyped 66 Prunus rootstock accessions and clones using chloroplast and nuclear microsatellites in this study. Chloroplast microsatellites revealed that the accessions belong to four previously defined maternal lineage groups (MLG-1 to -4) and five new ones (MLG-9 to -13). MLG-1 and -2 share the same chloroplast alleles of ‘Chinese Cling’ peach (Prunus persica) derived scions and American scions and rootstocks related to early European introductions, respectively. MLG-3 included ‘Guardian’ rootstock and its descendants. MLG-4 had a single genotype, ‘Okinawa’, that is the maternal parent of ‘Flordaking’. MLG-9 and MLG-11 to -13 included hybrids with different plums (Prunus salicina, Prunus cerasifera, Prunus tomentosa, or Prunus angustifolia) in their maternal parentage. MLG-10 included hybrids from almond (Prunus. dulcis) in the maternal parentage. The neighbor-joining phylogenetic tree based on nuclear microsatellite genotyping data showed several clusters. Cluster I included only one scion cultivar Elberta from MLG-1. Clusters II, III, and V contained peach accessions mostly in MLG-2. Clusters IV and VI included accessions mostly in MLG-3. Cluster VII included most accessions of plum-peach hybrid origin and those found within MLG-13. Cluster VIII was found to be mixed with different plum-peach hybrids and hybrids from other Prunus species, most of which were found in MLG-10, -11, and -12. Most accessions in Cluster IX were related to plums in MLG-11 and a few accessions in MLG-9.
美国农业部位于佐治亚州拜伦市的核果育种项目保留了一个李品种和杂交种的砧木集合。在本研究中,我们使用叶绿体和核微卫星对66个李砧木材料和克隆进行了基因分型。叶绿体微卫星显示,这些材料属于四个先前定义的母系谱系组(MLG-1至-4)和五个新的母系类群(MLG-9至-13)。MLG-1和-2分别与‘中国林’桃(Prunus persica)衍生的接穗和与早期欧洲引种有关的美国接穗和砧木具有相同的叶绿体等位基因。MLG-3包括“守护者”砧木及其后代。MLG-4具有单一基因型“Okinawa”,即“Flordaking”的母本。MLG-9和MLG-11-13包括在母系中具有不同李子(李、樱桃李、毛李或狭叶李)的杂交种。MLG-10包括母系中来自杏仁(Prunus.dulcis)的杂交种。基于核微卫星基因分型数据的邻居连接系统发育树显示了几个聚类。聚类I仅包括来自MLG-1的一个接穗品种Elberta。簇II、簇III和簇V包含主要在MLG-2中的桃材料。簇IV和簇VI包括主要在MLG-3中的材料。簇VII包括大多数李桃杂交来源的材料和在MLG-13中发现的材料。簇VIII被发现与不同的李-桃杂交种和其他李属物种的杂交种混合,其中大多数在MLG-10、-11和-12中发现。簇IX中的大多数材料与MLG-11中的李有关,少数材料与MLG-9有关。
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引用次数: 0
Root Absorption and Xylem Movement of Ethephon in Tomato 乙烯利在番茄中的根系吸收和木质部运动
IF 1.9 4区 农林科学 Q3 HORTICULTURE Pub Date : 2022-03-01 DOI: 10.21273/jashs05116-21
W. Miller, Wanxiang Lu, Dongqin Tang
Although ethephon is commonly used as a plant growth regulator during commercial production of horticultural crops, information on its movement within plants is limited. In this study, we developed a method to detect ethephon in plant tissues, and determined ethephon localization and movement using tomato (Solanum lycopersicum L.) as a model system. Tissues were ground in an acidic buffer that preserved ethephon intact. Ethylene was released from the extracts by adding sodium hydroxide and was determined subsequently by gas chromatography. Ethephon was detected in leaves within 1 hour of application to peat-based root zones and within 10 minutes in hydroponics. In a pulse–chase experiment, ethephon levels increased initially, then decreased after the plants were returned to ethephon-free solutions. Ethephon was present in directly collected xylem fluid; fluid collected from petiole stumps (after leaf blade excision) had similar ethephon levels between the different petioles. Stem girdling had no effect on ethephon accumulation in leaves. Together, these data indicate ethephon is readily mobile in the xylem stream and provides insight into the commercial use of ethephon as a root zone-applied growth regulator.
尽管乙烯利在园艺作物的商业化生产中通常被用作植物生长调节剂,但有关其在植物体内运动的信息有限。本研究建立了植物组织中乙烯利的检测方法,并以番茄(Solanum lycopersicum L.)为模型系统,确定了乙烯利的定位和运动。组织在酸性缓冲液中研磨,以保存乙烯利的完整。用氢氧化钠释放乙烯,用气相色谱法测定乙烯含量。在泥炭根区施用1小时内,在水培中施用10分钟内,在叶片中检测到乙烯利。在脉冲追逐实验中,乙烯利水平最初增加,然后在植物返回到不含乙烯利的溶液后下降。直接采集的木质部液中存在乙烯利;从叶柄残端(叶片切除后)收集的液体在不同叶柄之间具有相似的乙烯利水平。茎束对乙烯利在叶片中的积累没有影响。总之,这些数据表明乙烯利在木质部流中很容易移动,并为乙烯利作为根区应用生长调节剂的商业用途提供了见解。
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引用次数: 0
Temperature and Photoperiod Change the Flowering Process in Prunella vulgaris by Inducing Changes in Morphology, Endogenous Hormones, Carbon/Nitrogen Metabolites, and Gene Expression 温度和光周期通过诱导形态、内源激素、碳/氮代谢物和基因表达的变化来改变夏枯草的开花过程
IF 1.9 4区 农林科学 Q3 HORTICULTURE Pub Date : 2022-03-01 DOI: 10.21273/jashs05144-21
Zanzan Li, Jinyu Hu, H. Tang, L. Cao, Yu-hang Chen, Q. Guo, Chang-lin Wang
The spicas of Prunella vulgaris are widely used in the medical, beverage, and ornamental fields. Temperature and photoperiod are the two main ecological factors that determine the transformation of many plants from vegetative growth to reproductive growth. To explore the response of P. vulgaris flowering to temperature and photoperiod induction, we adopted vernalization long-day, vernalization short-day, nonvernalization long-day, and nonvernalization short-day treatments. The results showed that the morphology (total number of leaves, number of branches, number of leaves per branch, and branch length) of the vernalization treatment groups was significantly different from that of other nonvernalization groups, and the photosynthetic pigments, net photosynthetic rate, water use efficiency, stomatal conductance, intercellular CO2 concentration, and transpiration rate increased in the vernalization treatment group. However, the gibberellin 3 (GA3), indole-3-acetic acid and zeatin riboside (ZR) contents were significantly increased under the short-day treatments groups, and the results were the same for the expression of endogenous hormone synthesis genes, except for abscisic acid (ABA). The flowering-related genes soc1, elf3, svp, ga20ox, and cry1 were highly expressed under the vernalization short-day. Therefore, the induction of vernalization is more conducive to the increase in the photosynthetic rate. Temperature and photoperiod synergistically induced the synthesis and accumulation of starch, sugar, amino acids, and protein and affected the content of endogenous hormones and the expression of genes involved in their synthesis. GA3 and ZR had thresholds for their regulation of the flowering process in P. vulgaris, and high concentrations of ABA promoted flowering. Temperature and photoperiod coordinate the expression of the flowering-related genes soc1, elf3, svp, ga20ox, and cry1, thereby affecting the flowering process in P. vulgaris.
夏枯草的五倍子广泛应用于医药、饮料、观赏等领域。温度和光周期是决定许多植物从营养生长向生殖生长转变的两个主要生态因素。为了探讨寻常草开花对温度和光周期诱导的反应,我们采用了春化长日、春化短日、非春化长天和非春化短天处理。结果表明,春化处理组的形态(总叶数、分枝数、每分枝叶数和分枝长度)与其他非春化组有显著差异,光合色素、净光合速率、水分利用效率、气孔导度、细胞间CO2浓度,春化处理组蒸腾速率增加。然而,短日处理组的赤霉素3(GA3)、吲哚-3-乙酸和玉米素核糖(ZR)含量显著增加,除脱落酸(ABA)外,内源激素合成基因的表达结果相同。开花相关基因soc1、elf3、svp、ga20ox和cry1在春化短日条件下高表达。因此,春化的诱导更有利于光合速率的提高。温度和光周期协同诱导淀粉、糖、氨基酸和蛋白质的合成和积累,并影响内源性激素的含量和参与其合成的基因的表达。GA3和ZR对P.vulgaris的开花过程具有调控阈值,高浓度的ABA促进了其开花。温度和光周期协调开花相关基因soc1、elf3、svp、ga20ox和cry1的表达,从而影响P.vulgaris的开花过程。
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引用次数: 0
Photosynthetic Responses of Greenhouse Ornamentals to Interaction of Irradiance, Carbon Dioxide Concentration, and Temperature 温室观赏植物对光照、二氧化碳浓度和温度互作的光合响应
IF 1.9 4区 农林科学 Q3 HORTICULTURE Pub Date : 2022-03-01 DOI: 10.21273/jashs05115-21
Ian K. Atkins, J. Boldt
Supplemental lighting, temperature control, and CO2 enrichment can improve the productivity of greenhouse crops, but operating costs for greenhouse control systems to maintain environmental parameters at desired setpoints can be expensive. To balance operating costs with productivity, growers need to be able to predict how a crop will perform as a function of photosynthetic photon flux density (PPFD), CO2 concentration, and temperature. The objective of this study was to explore the response of net photosynthetic rate (Pn) to PPFD and CO2 concentration, for plants acclimated to different growth environment temperatures or light intensities. We measured Pn at all combinations of 14 irradiances and four CO2 concentrations of calibrachoa (Calibrachoa ×hybrida ‘Superbells Lemon Slice’), petunia (Petunia ×hybrida ‘Supertunia Mini Strawberry Pink Veined’), and verbena (Verbena ×hybrida ‘Superbena Royale Whitecap’) grown at three light intensities, and of geranium (Pelargonium ×hortorum ‘Maverick Red’), pepper (Capsicum annuum ‘California Wonder’), and sunflower (Helianthus annuus ‘Pacino Gold’) grown at three different temperatures. Sunflower, pepper, and geranium were fit to a model representing Pn as a function of PPFD, CO2 concentration, and leaf temperature. Photosynthetic light response curves, at each CO2 concentration, were fit for each species and growth environment using a nonrectangular hyperbola. These models can be used to identify multiple combinations of PPFD, CO2 concentration, and leaf temperature that would result in equivalent rates of photosynthesis, allowing the most cost-effective combination to be chosen.
补充照明、温度控制和二氧化碳富集可以提高温室作物的生产力,但温室控制系统将环境参数保持在所需设定点的操作成本可能很高。为了平衡运营成本和生产力,种植者需要能够预测作物的表现,作为光合光子通量密度(PPFD)、二氧化碳浓度和温度的函数。本研究的目的是探索适应不同生长环境温度或光照强度的植物的净光合速率(Pn)对PPFD和CO2浓度的响应。我们测量了在三种光照强度下生长的calbrachoa(calibrachoa×hybrida‘Superbells Lemon Slice’)、矮牵牛(petunia×hyhybrida’Supertunia Mini Strawberry Pink Veined’)和马鞭草(verbena×hybrida‘Superbena Royale Whitecap’)的14种辐射和四种CO2浓度的所有组合下的Pn,以及天竺葵(Pelargonium×hortorum‘Maverick Red’)的Pn,辣椒(Capsicum annuum‘California Wonder’)和向日葵(Helianthus annuus‘Pacino Gold’)在三种不同温度下生长。向日葵、胡椒和天竺葵被拟合到一个模型中,该模型将Pn表示为PPFD、CO2浓度和叶片温度的函数。在每个CO2浓度下,使用非矩形双曲线拟合每个物种和生长环境的光合光响应曲线。这些模型可用于识别PPFD、CO2浓度和叶片温度的多种组合,这些组合将产生相等的光合作用速率,从而选择最具成本效益的组合。
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引用次数: 0
Piloting a full-year, optics-based high school course on quantum computing. 试行基于光学的全年量子计算高中课程。
4区 农林科学 Q3 HORTICULTURE Pub Date : 2022-03-01 Epub Date: 2021-12-29 DOI: 10.1088/1361-6552/ac3dc2
Joel A Walsh, Mic Fenech, Derrick L Tucker, Catherine Riegle-Crumb, Brian R La Cour

Quantum computing was once regarded as a mere theoretical possibility, but recent advances in engineering and materials science have brought practical quantum computers closer to reality. Currently, representatives from industry, academia, and governments across the world are working to build the educational structures needed to produce the quantum workforce of the future. Less attention has been paid to growing quantum computing capacity at the high school level. This article details work at The University of Texas at Austin to develop and pilot the first full-year high school quantum computing class. Over the course of two years, researchers and practitioners involved with the project learned several pedagogical and practical lessons that can be helpful for quantum computing course design and implementation at the secondary level. In particular, we find that the use of classical optics provides a clear and accessible avenue for representing quantum states and gate operators and facilitates both learning and the transfer of knowledge to other Science, Technology, and Engineering (STEM) skills. Furthermore, students found that exploring quantum optical phenomena prior to the introduction of mathematical models helped in the understanding and mastery of the material.

量子计算曾一度被认为只是一种理论上的可能性,但最近在工程学和材料科学方面取得的进展使实用量子计算机更接近现实。目前,来自全球工业界、学术界和政府的代表正在努力建立培养未来量子人才所需的教育结构。在高中阶段提高量子计算能力的工作关注较少。本文详细介绍了德克萨斯大学奥斯汀分校为开发和试行首个全年制高中量子计算课程所做的工作。在两年的时间里,参与该项目的研究人员和从业人员学到了一些教学和实践经验,这些经验对中学量子计算课程的设计和实施很有帮助。特别是,我们发现经典光学的使用为表现量子态和门运算符提供了一个清晰易懂的途径,有利于学习和将知识迁移到其他科学、技术和工程(STEM)技能中。此外,学生们发现,在引入数学模型之前探索量子光学现象有助于理解和掌握教材。
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引用次数: 0
Burn Shock and Resuscitation: Review and State of the Science. 烧伤休克与复苏:科学回顾与现状》。
IF 1.4 4区 农林科学 Q3 HORTICULTURE Pub Date : 2022-02-26 DOI: 10.1093/jbcr/irac025
Robert Cartotto, David M Burmeister, John C Kubasiak

Burn shock and acute fluid resuscitation continue to spark intense interest and debate among burn clinicians. Following a major burn injury, fluid resuscitation of burn shock is life-saving, but paradoxically can also be a source of increased morbidity and mortality because of the unintended consequence of systemic edema formation. Considerable research over the past two decades has been devoted to understanding the mechanisms of edema formation, and to develop strategies to curb resuscitation fluids and limit edema development. Recognition of burn endotheliopathy - injury to the endothelium's glycocalyx layer- is one of the most important recent developments in our understanding of burn shock pathophysiology. Newer monitoring approaches and resuscitation endpoints, along with alternative resuscitation strategies to crystalloids alone, such as administration of albumin, or plasma, or high dose ascorbic acid, have had mixed results in limiting fluid creep. Clear demonstration of improvements in outcomes with all of these approaches remains elusive. This comprehensive review article on burn shock and acute resuscitation accompanies the American Burn Association's State of the Science meeting held in New Orleans, LA on November 2-3, 2021 and the Proceedings of that conference published in this journal.

烧伤休克和急性液体复苏一直是烧伤临床医生关注和争论的焦点。大面积烧伤后,对烧伤休克进行液体复苏可以挽救生命,但矛盾的是,由于系统性水肿形成的意外后果,液体复苏也可能成为增加发病率和死亡率的根源。在过去的二十年中,大量研究致力于了解水肿形成的机制,并开发出抑制复苏液体和限制水肿发展的策略。烧伤内皮病变--内皮糖萼层损伤--是我们了解烧伤休克病理生理学的最重要的最新进展之一。较新的监测方法和复苏终点,以及晶体液以外的其他复苏策略,如给予白蛋白、血浆或大剂量抗坏血酸,在限制液体蠕动方面的效果不一。所有这些方法在改善预后方面都没有明显的效果。这篇关于烧伤休克和急性复苏的综合评论文章随同美国烧伤协会于 2021 年 11 月 2-3 日在洛杉矶新奥尔良举行的 "科学现状 "会议以及该会议在本杂志上发表的论文集一起发表。
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引用次数: 0
Transcriptome-wide Identification and Characterization of microRNAs and Their Targets in a Highly Adaptable Conifer Platycladus orientalis 高适应性侧柏中微小RNA及其靶标的转录组鉴定与表征
IF 1.9 4区 农林科学 Q3 HORTICULTURE Pub Date : 2022-01-01 DOI: 10.21273/jashs05091-21
Ying Yang, Xian-Ge Hu, Bingsong Zheng, Yue Li, Tongli Wang, Anket Sharma, Huwei Yuan, Jian-Feng Mao
MicroRNAs (miRNAs) are short noncoding RNAs (20–25 nucleotides) that regulate gene expression posttranscriptionally. However, identification and characterization of miRNAs remain limited for conifer species. In this study, we applied transcriptome-wide miRNAs sequencing to a conifer species Platycladus orientalis, which is highly adaptable to a wide range of environmental adversities, including drought, barren soil, and mild salinity. A total of 17,181,542 raw reads were obtained from the Illumina sequencing platform; 31 conserved and 91 novel miRNAs were identified, and their unique characteristics were further analyzed. Ten randomly selected miRNAs were validated by quantificational real-time polymerase chain reaction. Through miRNA target predictions based on psRNATarget, 2331 unique mRNAs were predicted to be targets of P. orientalis miRNAs that involved in 187 metabolic pathways in KEGG database. These targets included not only important transcription factors (e.g., class III homeodomain leucine zipper targeted by por-miR166d) but also indispensable nontranscriptional factor proteins (i.e., por-miR482a-3p regulated nucleotide-binding site leucine-rich repeat protein). Interestingly, six miRNAs (por-miR16, -miR44, -miR60-5p, -miR69–3p, -miR166b-5p, and -miR395c) were found in adaptation-related pathways (e.g., drought), indicating their possible involved in this species’ stress-tolerance characteristics. The present study provided essential information for understanding the regulatory role of miRNAs in P. orientalis and sheds light on their possible use in tree improvement for stress tolerance.
微小RNA(miRNA)是一种短的非编码RNA(20-25个核苷酸),可在转录后调节基因表达。然而,对于针叶树物种来说,miRNA的鉴定和表征仍然有限。在这项研究中,我们将转录组范围的miRNA测序应用于针叶树物种侧柏,侧柏高度适应各种环境逆境,包括干旱、贫瘠土壤和轻度盐度。从Illumina测序平台获得总共17181542个原始读数;鉴定了31个保守的和91个新的miRNA,并进一步分析了它们的独特特征。10个随机选择的miRNA通过定量实时聚合酶链反应进行验证。通过基于psRNATarget的miRNA靶点预测,在KEGG数据库中,2331个独特的mRNA被预测为东方假单胞菌miRNA的靶点,这些miRNA参与187种代谢途径。这些靶标不仅包括重要的转录因子(例如,由por-miR166d靶向的III类同源结构域亮氨酸拉链),还包括不可或缺的非转录因子蛋白(即,por-miR482a-3p调节的核苷酸结合位点富含亮氨酸的重复蛋白)。有趣的是,在适应相关途径(如干旱)中发现了六种miRNA(por-miR16、-miR44、-miR60-5p、-miR69-3p、-mmiR166b-5p和-miR395c),表明它们可能参与了该物种的应激耐受特征。本研究为理解miRNA在东方松中的调节作用提供了重要信息,并阐明了它们在改善树木抗逆性方面的可能用途。
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引用次数: 2
期刊
Journal of the American Society for Horticultural Science
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