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Sensitivity of Trypanosome Isolates From Pigs In Enugu North Senatorial Zone of Enugu State To Diminazene Aceturate And Isometamidium Chloride 埃努古州北部参议院埃努古地区猪分离的锥虫对醋酸氨基苯和氯异甲酰胺的敏感性
Pub Date : 2018-12-01 DOI: 10.36108/jvbs/8102.10.0261
J. Omeje, J.S Akinbobols
The sensitivity of trypanosome isolates from naturally infected pigs in Enugu North Senatorial Zone was evaluated in mice at two dose levels each of diminazene aceturate (7 and 28 mg/kg body weight) and isometamidium chloride (0.25 and 2 mg/kg) using the infection and treatment methods. Multiple drug resistance was prevalent in the trypanosome isolates, as all 18 isolates (16 T. brucei and 2 T. congolense) tested were resistant to both diminazene aceturate (7 mg/kg b.w) and isometamidium chloride (0.25 mg/ kg b.w,), at the low dose levels tested. Sixteen of the isolates resisted the high dose levels of diminazene aceturate (28 mg/kg b.w), while six isolates were resistant to isometamidium chloride (2 mg/kg b.w). It was concluded that trypanosome isolates from pigs in the study area exhibited resistance to both diminazene aceturate and isometamidium chloride, the two most commonly used trypanocides in the area. This phenomenon constitutes serious threat to chemotherapeutic control of swine trypanosomosis in particular and animal trypanosomosis in general in Enugu North Senatorial Zone.
采用感染和治疗方法,对埃努古北参议院自然感染猪分离的锥虫在小鼠体内的敏感性进行了评估,分别给小鼠使用醋酸咪纳烯(7和28 mg/kg体重)和氯异甲酰胺(0.25和2 mg/kg)两个剂量水平。多重耐药在分离的锥虫中普遍存在,因为所有18株分离的锥虫(16株布鲁氏锥虫和2株刚果锥虫)在低剂量水平下均对醋酸氨基苯乙酯(7 mg/kg b.w)和氯异甲酰胺(0.25 mg/kg b.w)耐药。16株菌株对高剂量乙酸迪纳苯(28 mg/kg b.w)耐药,6株菌株对氯异异胺(2 mg/kg b.w)耐药。结果表明,研究区猪分离的锥虫对该地区最常用的两种锥虫杀虫剂醋酸二硝基甲苯和氯异异胺均有抗药性。这一现象对埃努古北参议院区猪锥虫病和动物锥虫病的化疗控制构成严重威胁。
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引用次数: 0
Prevalence of Parasitic Infections on Hematological Indices of Horses in the Gombe Royal Houses 贡贝王室马匹血液学指标寄生虫感染的流行情况
Pub Date : 2018-12-01 DOI: 10.36108/jvbs/8102.10.0251
Turaki U.A, Zaifada, A.U, Alkali, H.A, Galadima
The prevalence and effects of parasitic infections on hematological indices of horses in royal house of Gombe was studied. A total of 150 male horses were used for the study (January to March, 2017). Blood, feces and skin scrapings were collected from each horse and examined using various standard laboratory procedures. The coprological results showed that Ascaris accounted for 10% (15), flatworms (fasciola) 4% (6) and hookworms 4.6% (7). The skin scrapings, Mange accounted for 4 (2.6%), and ticks 8 (5.3%) while hemoparasitological analysis showed that Babesia accounted for 5 (3.33%). The blood indices showed that horses infected by parasites have significantly (p<0.05) lower RBC PCV, HB, and platelets compared to non infected horses while the WBC values increased significantly (p<0.05) due to immune-cellular response to the parasitic infection. The findings showed that parasitism is a problem in the stables examined and calls for proper stable hygiene, improved management practice, regular and strategic parasite monitoring and deworming programmes in order to achieve improved health and performance.
研究了贡贝王室马寄生虫感染的流行情况及其对血液指标的影响。研究共使用了150匹公马(2017年1月至3月)。从每匹马身上收集血液、粪便和皮肤刮痕,并使用各种标准实验室程序进行检查。血液学结果显示,其中蛔虫占10%(15只),扁虫(片形虫)占4%(6只),钩虫占4.6%(7只),皮肤刮痧、疥虫占4只(2.6%),蜱虫占8只(5.3%),巴贝虫占5只(3.33%)。血液指标显示,与未感染的马相比,感染寄生虫的马的红细胞PCV、HB和血小板显著(p<0.05)降低,而WBC则因免疫细胞对寄生虫感染的反应而显著升高(p<0.05)。调查结果表明,寄生虫是所检查的马厩中的一个问题,因此需要适当的马厩卫生、改进管理做法、定期和战略性的寄生虫监测和除虫方案,以便改善健康和业绩。
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引用次数: 0
Evaluation of The Antioxidant Potentials of African Nutmeg (Monodora Myristica) Extraction Palm Oil Stability 非洲肉豆蔻(Monodora Myristica)萃取物抗氧化活性及棕榈油稳定性的评价
Pub Date : 2018-12-01 DOI: 10.36108/jvbs/8102.10.0231
B. Mofio, O. Adeyanju
Palm Oil contains high content of carotenoids and is a rich source of vitamin E consisting of tocopherols and tocotrienols which are well known nutritional antioxidants. Lipid oxidation has seriously limited the stability of palm oil resulting in rancidity. Therefore this work evaluates the antioxidant potential of Monodora myristica (African nutmeg) on Palm oil stability. Monodora myristica extract was obtained by maceration technique using n-hexane as solvent. Equal volumes of Palm oil obtained from one source were treated with different volumes (0.2ml, 0.4ml, 0.6m, 0.8m, 1.0ml) of African Nutmeg extract using syringe. Two experimental groups were prepared. A group were placed under the sun (S.S) and the other group in a room (S.R) for a period of two weeks. These treated oil samples were analyzed by titration, on weekly basis to determine the free fatty acid content and acid value. Results obtained showed that for the two groups of treated Palm oil, the free fatty acid content and acid value increased significantly (P<0.05 and P<0.01) at lower volumes of extract and as the period (week) extends. The free fatty acid content and acid value of oil samples treated with higher extract volumes decreased significantly (P<0.05 and P<0.01) for both groups (S.S and S.R). However all the values were higher than pre-treatment values. Hence, Monodora myristica extract reduces effect of lipid oxidation on palm oil and consequently increases its stability.
棕榈油含有高含量的类胡萝卜素,是维生素E的丰富来源,维生素E由生育酚和生育三烯醇组成,这是众所周知的营养抗氧化剂。脂质氧化严重限制了棕榈油的稳定性,导致酸败。因此,本研究评价了非洲肉豆蔻对棕榈油稳定性的抗氧化潜力。以正己烷为溶剂,采用浸渍法制备肉豆汁单菌提取物。从一个来源获得等体积的棕榈油,用注射器处理不同体积(0.2ml, 0.4ml, 0.6m, 0.8m, 1.0ml)的非洲肉豆蔻提取物。实验组分为两组。一组放置在阳光下(S.S),另一组放置在房间(S.R),为期两周。对处理后的油样进行每周一次的滴定分析,测定游离脂肪酸含量和酸值。结果表明,两组棕榈油处理后,随着提取量的降低和处理时间(周)的延长,游离脂肪酸含量和酸值均显著升高(P<0.05和P<0.01)。高抽提量处理各组(S.S和S.R)的游离脂肪酸含量和酸值均显著降低(P<0.05和P<0.01)。但所有数值均高于预处理值。因此,肉豆蔻单酵母菌提取物减少了脂质氧化对棕榈油的影响,从而提高了棕榈油的稳定性。
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引用次数: 0
A Survey of Trypanosome Species Circulating In Pigs In Three Selected Local Government District of Enugu State, Nigeria 尼日利亚埃努古州三个选定的地方政府区猪中流行的锥虫物种调查
Pub Date : 2018-12-01 DOI: 10.36108/jvbs/8102.10.0241
J. Omeje, J. Akinbobola
A cross sectional survey of trypanosomosis of pigs was carried out in three randomly selected Local Government Areas (Nsukka, Udenu and Igbo Eze South) out of the six local government areas in Enugu North Senatorial Zone. Blood samples were randomly collected from a total of 1800 pigs from abattoirs and farms and were examined for trypanosomes parasites using standard parasitological methods: wet film, buffy coat and Giemsa-stained thin smear preparations. A total of 93 (5.2%) pigs were positive overall. Among the positive samples, Trypanosoma brucei was the predominant species (86.0%), followed by T. congolense (13.9%). The different relative isolation rate for Nsukka, Igbo-Eze South and Udenu Local government areas were 14 (4.7%), 14 (4.7%), and 19 (4.0%) respectively in the dry season, and 17 (5.7%), 17 (5.7%) and 12 (6.3%) in the rainy season respectively. The trypanosome prevalence in males from Nsukka, Igbo-Eze South and Udenu local governments were 6.5%, 6.2% and 4.2% respectively, while report in female were 3.8%, 4.1% and 5.9% in the three local governments respectively. There was no significant variations (p<0.05) with seasons, sex and age groups. It was therefore concluded that porcine trypanosomosis is still endemic in Enugu North Senatorial Zone with T. brucei being the most incriminated as the cause of the disease in the zone.
在埃努古北部参议院区6个地方政府区中随机选择的3个地方政府区(恩苏卡、乌德努和南伊博埃泽)开展了猪锥虫病的横断面调查。从屠宰场和农场随机收集了总共1800头猪的血液样本,并使用标准的寄生虫学方法进行了锥虫寄生虫检查:湿膜、黄皮毛和吉姆萨染色薄涂片制剂。总体上共有93头猪(5.2%)呈阳性。阳性标本中,优势种为布氏锥虫(86.0%),其次为刚果锥虫(13.9%)。Nsukka、Igbo-Eze南部和Udenu地方政府区旱季相对隔离率分别为14(4.7%)、14(4.7%)和19(4.0%),雨季相对隔离率分别为17(5.7%)、17(5.7%)和12(6.3%)。恩苏卡、伊博-埃泽南部和乌德努地方政府男性锥虫患病率分别为6.5%、6.2%和4.2%,女性分别为3.8%、4.1%和5.9%。季节、性别、年龄组间无显著差异(p<0.05)。因此得出结论,猪锥虫病在埃努古北参议院区仍然是地方性的,布鲁氏体是该地区最常见的病因。
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引用次数: 0
Histological Study On The Testes of African Striped Ground Squirrel (Xerus Erythropus) 非洲斑地鼠睾丸的组织学研究
Pub Date : 2018-12-01 DOI: 10.36108/jvbs/8102.10.0280
R. M. Korzerzer
In this study, the testes of African striped ground squirrels (Xerus erythropus)obtained between the months of January and May were studied at histological level using Haematoxilin and Eosin stain, Masson Trichome stain and Periodic acid Schiff to examine the histo-architechure, collagen and glycogen localizations in the testes respectively. The results showed that the testes had finely arranged cells within the seminiferous tubules which were positive for both Masson-trichome and Periodic acid shiff by reacting to the blue and magenta colours respectively. However, the histoarchitechture of the testes did not show spermatogenic activity. In conclusion, the testes of African striped ground squirrel obtained between the months of January and May did not show detailed spermatogenic activity. This suggests that the squirrel is a seasonal breeding rodent with a dormant reproductive phase occurring around January to May. The testes also contains cells that express glycogen which suggests that energy in form of glycogen may be needed for the initiation of spermatogenesis.
本研究对1 ~ 5月采集的非洲条纹地鼠睾丸进行了组织学水平的研究,分别采用血红素和伊红染色、马松毛斑染色和周期性酸Schiff染色检测了睾丸的组织结构、胶原蛋白和糖原定位。结果表明,睾丸精小管内细胞排列精细,对蓝色和品红色分别有马松-毛状体和周期性酸移阳性反应。然而,睾丸组织结构未显示生精活性。总之,1月至5月间获得的非洲条纹地鼠睾丸未显示出详细的生精活性。这表明松鼠是一种季节性繁殖的啮齿动物,其休眠繁殖阶段发生在1月至5月左右。睾丸也含有表达糖原的细胞,这表明糖原形式的能量可能需要精子发生的开始。
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引用次数: 0
Ultrasonographic, Hormonal, Morphological And Morphometric Correlate Studies of Induced Testicular Pathology In West African Dwarf Goat Bucks. 西非矮山羊雄鹿诱导睾丸病理的超声、激素、形态学和形态计量学相关性研究。
Pub Date : 2018-12-01 DOI: 10.36108/jvbs/8102.10.0211
L. Raji
The study was carried out to evaluate the significance of ultrasound in the early detection of testicular pathology in the West African Dwarf (WAD) buck; hormonal, morphological andmorphometric studies were used as correlates. Twelve matured WAD bucks divided into two groups (of 6 per group); A—control, B — bucks with right testis spermatic cord ligated for four weeks were used for this study. Testicular ultrasound protocols were carried on the Longitudinal Planes (LP) and Transverse Planes (TP) on both testes. Blood samples were collected via the jugular vein twice a week for four weeks and assayed for testosterone, Luteinizing Hormone (LH) and Follicle Stimulation Hormone (FSH) concentrations. The testes were harvested after four weeks and carefully separated from the epididymides for gross and histological studies. Results revealed that 24 hours post induction of testicular pathology, inflammation of the right testes of the group B bucks was detected by ultrasound as hypo-echoic (dark) regions covering about two-third of the entire testis while the non-ligated testes appeared mostly homogeneously greyish on the TP. By the end of the fourth week the entire ligated testes were inflamed and appeared hypo-echoic with some areas of fibrosis on the TP and LP. The LH and FSH concentrations increased while there were no significant changes in testosterone concentrations. There were marked reduction in testicular and epididymal weights and sizes in group B bucks. The group B bucks testes showed complete loss of testicular architecture while the Leydig cells were intact. In conclusion, testicular ultrasound was valuable in the early detection of the induced testicular pathology (which hormonal, morphological and morphometric studies confirmed), hence should be adopted as part of the BSE programme of the WAD buck.
本研究旨在评价超声在西非矮雄(WAD)雄鹿睾丸病理早期检测中的意义;激素、形态学和形态计量学研究被用作相关性研究。12只成熟的WAD美元分成两组(每组6只);a组对照,B组雄鹿,右睾丸精索结扎四周。在两个睾丸的纵向平面(LP)和横向平面(TP)上进行睾丸超声检查。每周两次经颈静脉采血,持续四周,检测睾酮、促黄体生成素(LH)和促卵泡激素(FSH)浓度。4周后收集睾丸,仔细地与附睾分离,进行大体和组织学研究。结果显示,病理诱导后24小时,B组雄鼠右侧睾丸超声示低回声(暗)区,约占整个睾丸的三分之二,未结扎的睾丸在TP上大部分呈均匀的灰色。到第四周结束时,结扎的整个睾丸发炎,出现低回声,TP和LP上有一些纤维化区域。黄体生成素和卵泡刺激素浓度升高,而睾酮浓度无明显变化。B组雄鼠睾丸和附睾的重量和大小明显减少。B组雄雄睾丸结构完全丧失,而间质细胞完好无损。总之,睾丸超声在早期发现诱发性睾丸病理(激素、形态学和形态计量学研究证实)方面是有价值的,因此应作为WAD buck的BSE计划的一部分。
{"title":"Ultrasonographic, Hormonal, Morphological And Morphometric Correlate Studies of Induced Testicular Pathology In West African Dwarf Goat Bucks.","authors":"L. Raji","doi":"10.36108/jvbs/8102.10.0211","DOIUrl":"https://doi.org/10.36108/jvbs/8102.10.0211","url":null,"abstract":"The study was carried out to evaluate the significance of ultrasound in the early detection of testicular pathology in the West African Dwarf (WAD) buck; hormonal, morphological and\u0000morphometric studies were used as correlates. Twelve matured WAD bucks divided into two groups (of 6 per group); A—control, B — bucks with right testis spermatic cord ligated for four weeks were used for this study. Testicular ultrasound protocols were carried on the Longitudinal Planes (LP) and Transverse Planes (TP) on both testes. Blood samples were collected via the jugular vein twice a week for four weeks and assayed for testosterone, Luteinizing Hormone (LH) and Follicle Stimulation Hormone (FSH) concentrations. The testes were harvested after four weeks and carefully separated from the epididymides for gross and histological studies. Results revealed that 24 hours post induction of testicular pathology, inflammation of the right testes of the group B bucks was detected by ultrasound as hypo-echoic (dark) regions covering about two-third of the entire testis while the non-ligated testes appeared mostly homogeneously greyish on the TP. By the end of the fourth week the entire ligated testes were inflamed and appeared hypo-echoic with some areas of fibrosis on the TP and LP. The LH and FSH concentrations increased while there were no significant changes in testosterone concentrations. There were marked reduction in testicular and epididymal weights and sizes in group B bucks. The group B bucks testes showed complete loss of testicular architecture while the Leydig cells were intact. In conclusion, testicular ultrasound was valuable in the early detection of the induced testicular pathology (which hormonal, morphological and morphometric studies confirmed), hence should be adopted as part of the BSE programme of the WAD buck.","PeriodicalId":17442,"journal":{"name":"Journal of Veterinary and Biomedical Sciences","volume":"243 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86703382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Detection of Ochratoxin A From Poultry Feed Using High Pressure Liquid Chromatography 高压液相色谱法检测家禽饲料中的赭曲霉毒素A
Pub Date : 2018-12-01 DOI: 10.36108/jvbs/8102.10.0230
L. Adeniran
One hundred poultry feed samples comprising of commercially produced poultry feed (48) and poultry feed compounded by farmers (privately milled) (52) were collected from farms located in Minna and analysed for Ochratoxin A (OTA), a member of group 2 possible carcinogen by High Pressure Liquid Chromatographic (HPLC) method. Thirty seven percent of the commercial poultry feeds were contaminated with OTA at a range of 0 -236.73ug/kg while hundred percent of privately made feed were contaminated with OTA at a range of 22.76-226.5lug/kg. The finding of this investigation showed that 71% (71/100) of the sampled poultry feed has OTA concentrations which was far in excess of the maximum permissible limit of 5ug/kg (the European Union Standard). This is of serious health concern to the birds and humans that consume the poultry products.
从Minna的农场收集了100个家禽饲料样本,包括商业生产的家禽饲料(48个)和农民配制的家禽饲料(52个),并使用高压液相色谱(HPLC)方法分析了赭曲霉菌毒素A (OTA),这是第2组可能的致癌物。37%的商业家禽饲料中OTA的污染范围为0 -236.73ug/kg,而100%的自制饲料中OTA的污染范围为22.76-226.5 ug/kg。调查结果显示,71%(71/100)的抽样家禽饲料的OTA浓度远远超过5微克/公斤(欧盟标准)的最大允许限量。这对食用家禽产品的禽类和人类来说是一个严重的健康问题。
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引用次数: 0
Estrous Cycle of Induced Ovulators: Lesson From The Camel — A Review 诱导排卵者的发情周期:来自骆驼的教训-综述
Pub Date : 2018-12-01 DOI: 10.36108/jvbs/8102.10.0221
Y. Dauda
Camel, rabbit, cat, ferrets, minks, koala and meadow moles are induced ovulators requiring copulation to trigger the ovulatory process and the estrous cycle differs from that of other domestic animals. The estrous cycle in these animals composed of follicular recruitment, follicular growth, follicular maturity and follicular regression phase. These animals are variously reared as companion, fur-bearing and meat animals. Among these, the camel is the most valuable and classical induced ovulator which is rear not only for milk and meat, but as work animal and contributes effectively to the welfare of people in harsh and difficult environments. As a classical induced ovulatory, camelid has cycling receptivity with distinctive estrus but requires mating in order to ovulate. The other classes of induced ovulators like cats and ferrets require both the presence of male to achieve behavioral estrus and actual copulation to ovulate. The camel has good prospects of survival as a suitable livestock for projects of sustainable agriculture and animal production under harsh desert or arid conditions. However the reproductive nature of camels presents a huge challenge to camel husbandry. The natural constraints include the long period of attaining puberty, limited breeding season, difficulties in induced ovulation, long gestation period and inter-calving intervals. Efforts to improve the reproductive efficiency of the female camel are closely related to a better understanding of the folliculogenesis or follicular wave pattern. Many investigators might not be aware of the peculiar reproductive information available about this animal species. A working knowledge of ovarian function or estrous cycle will be of immense importance to the application of assisted reproductive technologies (ARTs) and enhancements of reproduction in camelids. This work presents the overview of estrous cycle in camel as a classical example of induced ovulators with the aim of providing current knowledge to the reader and to stimulate wider research interest in camel research and reproduction.
骆驼、兔子、猫、雪貂、水貂、考拉、草鼠等都是诱导排卵动物,需要通过交配来触发排卵过程,其发情周期与其他家畜不同。这些动物的发情周期由卵泡募集期、卵泡生长期、卵泡成熟期和卵泡退行期组成。这些动物被饲养成各种各样的伴侣动物、毛皮动物和食肉动物。其中,骆驼是最有价值和最经典的促排卵动物,它不仅为奶和肉而生,而且作为劳动动物,在恶劣和困难的环境中为人类的福利做出了有效的贡献。骆驼是一种典型的诱导排卵动物,具有周期性接受性,具有独特的发情期,但需要交配才能排卵。其他种类的诱导排卵动物,如猫和雪貂,既需要雄性的存在来实现行为发情,也需要实际的交配来排卵。在恶劣的沙漠或干旱条件下,骆驼作为可持续农业和动物生产项目的合适牲畜,具有良好的生存前景。然而,骆驼的繁殖特性给骆驼养殖业带来了巨大的挑战。自然的制约因素包括:进入青春期时间长、繁殖季节有限、诱导排卵困难、妊娠期长、产犊间隔长。提高雌性骆驼繁殖效率的努力与更好地了解卵泡发生或卵泡波型密切相关。许多研究人员可能没有意识到这种动物特有的生殖信息。了解卵巢功能或发情周期对辅助生殖技术的应用和提高骆驼的生殖能力具有重要意义。本研究概述了骆驼的发情周期,作为诱导排卵的一个经典例子,旨在为读者提供当前的知识,并激发对骆驼研究和繁殖的更广泛的研究兴趣。
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引用次数: 0
Co-infection Dynamics of Canine Parasitic Diseases In Osun State, Nigeria: A Retrospective Approach. 尼日利亚奥孙州犬寄生虫病的合并感染动态:回顾性方法。
Pub Date : 2018-12-01 DOI: 10.36108/jvbs/8102.10.0260
S. Ola-Fadunsin
Multiple parasitic co-infections are a normal occurrence in animals. A ten year (2006-2015) data of parasitic diseases diagnosed in the major Veterinary clinics in Osun state was collected and subjected to statistical analysis. The prevalence of parasitic diseases co-infection, the epidemiological risk factors and the Spearman’s correlation coefficient associated with parasitic diseases co-infection was carried out to determine the dynamics of canine parasitic diseases in Osun State. A significant number of dogs were diagnosed with two or more parasitic infections at the same time. Exotic breed of dogs recorded a significant (P<0.05) lower prevalence of parasitic disease co-infections compared to indigenous and cross breeds. Young dogs were more prone to multiple parasite co-infections compared to growing and adult dogs. There was no significant difference (P>0.05) within gender as it pertains to multiple parasitic diseases co-infection. Higher number of parasite co-infections was recorded during the wet season than the dry season. There was a positive correlation between the occurrence of tick infestation with babesiosis, and tick infestation with ehrlichiosis. There is need for an improved Veterinary medical attention and hygienic practices given to dogs in the study area as multiple parasitic diseases co-infection could cause severe and adverse effect on the health, reproduction and performance of dogs.
多种寄生虫合并感染在动物中是正常现象。收集奥松州主要兽医诊所10年(2006-2015年)寄生虫病诊断数据并进行统计分析。为确定奥松州犬寄生虫病的动态,开展了寄生虫病合并感染的流行病学风险因素和与寄生虫病合并感染相关的斯皮尔曼相关系数的调查。相当数量的狗同时被诊断出患有两种或两种以上的寄生虫感染。外来犬种对多种寄生虫病合并感染的性别差异有统计学意义(P0.05)。在雨季记录的寄生虫合并感染数量高于旱季。蜱虫感染与巴贝斯虫病、蜱虫感染与埃立克体病呈正相关。由于多种寄生虫疾病的合并感染可能对犬的健康、繁殖和生产性能造成严重的不利影响,因此需要改进对研究地区犬类的兽医医疗关注和卫生规范。
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引用次数: 0
Pathologic Diagnosis of Infectious Bursal Disease Outbreak In Vaccinated 3-week Old Pullet Chicks on A Small Scale Commercial Poultry Farm In Leleyi-kwali, Abuja, Nigeria: A Case Report 尼日利亚阿布贾Leleyi-kwali一家小规模商业家禽养殖场接种疫苗的3周龄小鸡传染性法氏囊病暴发的病理学诊断:1例报告
Pub Date : 2018-12-01 DOI: 10.36108/jvbs/8102.10.0210
S. Abalaka
sudden mortality which lasted for seven days with the peak on the third day, was investigated in vaccinated 3-week old pullet chicks. Signs of depression, ruffled feathers, sneezing, huddling together and pasted vents with about 50% morbidity were observed. While chicks were off feed. Vaccination history against Marek’s and Newcastle diseases was reportedly up to date. The first dose of Infectious Bursal Disease (IBD) vaccination was done 12 days before the onset of the outbreak preparatory to the second dose. Detailed post mortem examinations showed multifocal petechial to ecchymotic haemorrhages on the breast and thigh muscles with swollen, oedematous and haemorrhagic bursa of Fabricius in affected chicks. The bursa of Fabricius showed diffuse lymphoid depletion with multifocal vacuolations and eosinophilic cystic follicles. Similar diffuse lymphoid depletion and cytoplasmic vacuolation with fibrinous exudation were also present in then spleen, Serological analysis revealed 88.23% IBD antibody positive sera in affected pullets 16 days after cessation of mortality. A diagnosis of Infectious Bursal Disease outbreak was made based on the observed pathological presentations along with the clinical signs, typical spiked mortality pattern, and serological titre. These findings highlighted the need for continuous pathological investigations as a ready diagnostic tool in vaccinated and unvaccinated pullet chicks in Nigeria where Infectious bursal disease has become endemic.
对接种疫苗的3周龄雏鸡进行了突然死亡调查,死亡率持续7天,第3天达到高峰。观察到抑郁、皱起羽毛、打喷嚏、挤在一起和粘贴通风口的迹象,发病率约为50%。当小鸡没有饲料时。据报道,马立克氏病和新城疫的疫苗接种史是最新的。传染性法氏囊病(IBD)的第一剂疫苗接种是在疫情爆发前12天进行的,为第二剂疫苗接种做准备。详细的尸检显示,受感染雏鸡的乳房和大腿肌肉有多灶点状到瘀斑性出血,伴有肿胀、水肿和出血的法氏囊。法氏囊表现为弥漫性淋巴细胞减少,伴多灶空泡和嗜酸性囊泡。在死亡停止后16天的血清学分析显示,患病雏鸡血清中有88.23%的IBD抗体阳性。根据观察到的病理表现、临床症状、典型的尖峰死亡模式和血清学滴度,做出传染性法氏囊病爆发的诊断。这些发现突出表明,在传染性法氏囊病已成为地方病的尼日利亚,有必要对接种疫苗和未接种疫苗的雏鸡进行持续的病理调查,作为一种现成的诊断工具。
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引用次数: 0
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