Pub Date : 2020-12-01DOI: 10.36108/jvbs/9102.20.0280
O. Oludairo
Methicillin-resistant Staphylococcus aureus (MRSA) has received a lot of attention in recent years as a zoonotic organism of global concern. Contaminated milk, especially those from mastitic cows, serve as reservoirs for humans in the epidemiology of antibiotic resistant MRSA. This study was designed to determine the level of contamination of bulk fresh milk from dairy cattle herds with MRSA in Ibarapa, Oyo and Oke-Ogun areas of Oyo State and the antibiotic resistance profile of the isolates. One hundred and sixty-five (165) milk samples were obtained from the study areas and used for the study. Staphylococcus aureus was isolated from the samples using bacterial culture and biochemical tests. Methicillin-resistant Staphylococcus aureus was identified using cefoxitin disk diffusion method. All the S. aureus isolates were subjected to microbial susceptibility test. Ninety (54.5%) milk samples were positive for Staphylococcus spp. out of which 52 (31.5%) were Staphylococcus aureus and 13 (7.9%) yielded MRSA. Antibiogram of S. aureus indicated highest resistance to Cloxacillin (88.5%) followed by (Augmentin 67.3%) and Ceftrazidine (67.3%). Ten out of the 13 MRSA isolates were multidrug resistance while all the isolates were 100% susceptible to ofloxacin. The results of this study showed that milk produced from dairy cattle in Oyo State was contaminated with MRSA. This portends serious food safety and public health risk among the consumers of such milk especially in raw or improperly pasteurized form. Proper dairy herd health management and prudent use of antibiotics and hygienic milking procedures are hereby recommended to prevent contamination of milk and subsequent spread of the organism to humans.
{"title":"Prevalence of Methicillin Resistant Staphylococcus aureus (MRSA) in Cattle Milk from dairy Herds in Oyo State, Nigeria","authors":"O. Oludairo","doi":"10.36108/jvbs/9102.20.0280","DOIUrl":"https://doi.org/10.36108/jvbs/9102.20.0280","url":null,"abstract":"Methicillin-resistant Staphylococcus aureus (MRSA) has received a lot of attention in recent years as a zoonotic organism of global concern. Contaminated milk, especially those from mastitic cows, serve as reservoirs for humans in the epidemiology of antibiotic resistant MRSA. This study was designed to determine the level of contamination of bulk fresh milk from dairy cattle herds with MRSA in Ibarapa, Oyo and Oke-Ogun areas of Oyo State and the antibiotic resistance profile of the isolates. One hundred and sixty-five (165) milk samples were obtained from the study areas and used for the study. Staphylococcus aureus was isolated from the samples using bacterial culture and biochemical tests. Methicillin-resistant Staphylococcus aureus was identified using cefoxitin disk diffusion method. All the S. aureus isolates were subjected to microbial susceptibility test. Ninety (54.5%) milk samples were positive for Staphylococcus spp. out of which 52 (31.5%) were Staphylococcus aureus and 13 (7.9%) yielded MRSA. Antibiogram of S. aureus indicated highest resistance to Cloxacillin (88.5%) followed by (Augmentin 67.3%) and Ceftrazidine (67.3%). Ten out of the 13 MRSA isolates were multidrug resistance while all the isolates were 100% susceptible to ofloxacin. The results of this study showed that milk produced from dairy cattle in Oyo State was contaminated with MRSA. This portends serious food safety and public health risk among the consumers of such milk especially in raw or improperly pasteurized form. Proper dairy herd health management and prudent use of antibiotics and hygienic milking procedures are hereby recommended to prevent contamination of milk and subsequent spread of the organism to humans.","PeriodicalId":17442,"journal":{"name":"Journal of Veterinary and Biomedical Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80657216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-12-01DOI: 10.36108/jvbs/9102.20.0220
M. M. Onakpa
Striga hermonthica is a ubiquitous hemi-parasitic plant commonly known as witch-weed and is used in West Africa as a traditional herbal medicine for the treatment of an array of diseases. In this study, methanol leaf extract of S. hermonthica was used to investigate the acute and sub-acute toxicity effects in male Wistar rats. In the acute toxicity studies, Wistar rats were divided into six groups comprising of negative control and extract treated groups (250, 500, 1000, 1500 and 2000 mg/kg of extract orally). The rats were observed for 72 hours while in the sub-acute oral toxicity studies, the rats were divided into 4 groups consisting of 5 rats per group. The extract was administered orally at doses of 25, 50 and 100 mg/kg daily for 28 days to groups II, III and IV respectively while group I (negative control) received 2 ml of distilled water. The dose of 2000 mg/kg did not cause any mortality or signs of toxicity in the treated rats during the acute and subacute toxicity studies did not show any treatment-related abnormalities in the hematological (RBC, Hb, WBC, Lymphocytes) and biochemical (AST, ALT, ALP, TB, Glucose, HDL, LDL, Total protein, Albumin) parameters while the liver revealed lesions in the histopathology studies, there were no treatment-related lesions observed in the heart, lungs, pancreas and kidney whereas, the weight of rats did not show significant difference (p > 0.05) between the control and the treated groups. The study showed that S. hermonthica caused hepatotoxicity and could be potentially harmful for use. A more comprehensive research is recommended to investigate on its safe use and mode of action.
{"title":"Toxicity Studies of Striga hermonthica (Delile) Benth Leaf Extract in Rats","authors":"M. M. Onakpa","doi":"10.36108/jvbs/9102.20.0220","DOIUrl":"https://doi.org/10.36108/jvbs/9102.20.0220","url":null,"abstract":"Striga hermonthica is a ubiquitous hemi-parasitic plant commonly known as witch-weed and is used in West Africa as a traditional herbal medicine for the treatment of an array of diseases. In this study, methanol leaf extract of S. hermonthica was used to investigate the acute and sub-acute\u0000toxicity effects in male Wistar rats. In the acute toxicity studies, Wistar rats were divided into six groups comprising of negative control and extract treated groups (250, 500, 1000, 1500 and 2000 mg/kg of extract orally). The rats were observed for 72 hours while in the sub-acute oral toxicity studies, the rats were divided into 4 groups consisting of 5 rats per group. The extract was administered orally at doses of 25, 50 and 100 mg/kg daily for 28 days to groups II, III and IV respectively while group I (negative control) received 2 ml of distilled water. The dose of 2000 mg/kg did not cause any mortality or signs of toxicity in the treated rats during the acute and subacute toxicity studies did not show any treatment-related abnormalities in the hematological (RBC, Hb, WBC, Lymphocytes) and biochemical (AST, ALT, ALP, TB, Glucose, HDL, LDL, Total protein, Albumin) parameters while the liver revealed lesions in the histopathology studies, there were no treatment-related lesions observed in the heart, lungs, pancreas and kidney whereas, the weight of rats did not show significant difference (p > 0.05) between the control and the treated groups. The study showed that S. hermonthica caused hepatotoxicity and could be potentially harmful for use. A more comprehensive research is recommended to investigate on its safe use and mode of action.","PeriodicalId":17442,"journal":{"name":"Journal of Veterinary and Biomedical Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85237009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-02-01DOI: 10.36108/jvbs/9102.20.0161
R. M. Korzerzer
In this study, ten African striped ground squirrels (ASGS) (Xerus erythropus) obtained in Zaria and environs with the aid of traps between January and May, 2017 were transported in iron cages to the research laboratory in the Department of Veterinary Anatomy, Ahmadu Bello University, Zaria. After acclimatization for twenty-one days, the animals were weighed separately using a weighing balance (Fuji Keiryo weighing scale K-1, 210 × 210, 200g – 8kg) and then Thiopental sodium was administered at the dose of 30mg/kg to euthanize the animals. The testes, epididymis, ductus deferens and penis were then obtained by making an abdomino-pelvic incision and exposing the organs. Each organ was studied grossly. Observations for shape, colour and general architecture were made and photographs taken using a digital camera(Samsung SH100, 14.2 megapixels). From this study the testes of ASGS is dark-red in colour. The left and right epididymis is closely attached on the left and right testis. Each epididymis is anatomically divided into catput, corpus and caudal epididymis. The caudal epididymis has two aspects as the fixed and free part of the caudal epididymis. The penis is long and slender with an average length of 8.5 cm, and appears white in colour. The glans is covered by an external prepuce in the non-erectile state. The baculum is a sickle shaped single bone on the glans, and it has an average length of 1.9 cm. The prostate gland is a single gland just below the urinary bladder, the bulbourethral gland is located in the ishio-carvenosus muscle at the crura of penis, and closely attached to the distal aspect of the pelvic urethra linking the penis. The seminal vesicles are paired, and oval shaped glands proximal to the urinary bladder. In conclusion, the gross appearance of the male reproductive organs of the ASGS has anatomic modifications which is suggestive of its mating tendencies in the wild.
{"title":"Macro-Anatomy of Male Reproductive Organs of African Striped Ground Squirrel (Xerus erythropus).","authors":"R. M. Korzerzer","doi":"10.36108/jvbs/9102.20.0161","DOIUrl":"https://doi.org/10.36108/jvbs/9102.20.0161","url":null,"abstract":"In this study, ten African striped ground squirrels (ASGS) (Xerus erythropus) obtained in Zaria and environs with the aid of traps between January and May, 2017 were transported in iron cages to the research laboratory in the Department of Veterinary Anatomy, Ahmadu Bello University, Zaria. After acclimatization for twenty-one days, the animals were weighed separately using a weighing balance (Fuji Keiryo weighing scale K-1, 210 × 210, 200g – 8kg) and then Thiopental sodium was administered at the dose of 30mg/kg to euthanize the animals. The testes, epididymis, ductus deferens and penis were then obtained by making an abdomino-pelvic incision and exposing the organs. Each organ was studied grossly. Observations for shape, colour and general architecture were made and photographs taken using a digital camera(Samsung SH100, 14.2 megapixels). From this study the testes of ASGS is dark-red in colour. The left and right epididymis is closely attached on the left and right testis. Each epididymis is anatomically divided into catput, corpus and caudal epididymis. The caudal epididymis has two aspects as the fixed and free part of the caudal epididymis. The penis is long and slender with an average length of 8.5 cm, and appears white in colour. The glans is covered by an external prepuce in the non-erectile state. The baculum is a sickle shaped single bone on the glans, and it has an average length of 1.9 cm. The prostate gland is a single gland just below the urinary bladder, the bulbourethral gland is located in the ishio-carvenosus muscle at the crura of penis, and closely attached to the distal aspect of the pelvic urethra linking the penis. The seminal vesicles are paired, and oval shaped glands proximal to the urinary bladder. In conclusion, the gross appearance of the male reproductive organs of the ASGS has anatomic modifications which is suggestive of its mating tendencies in the wild.","PeriodicalId":17442,"journal":{"name":"Journal of Veterinary and Biomedical Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76876712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-02-01DOI: 10.36108/jvbs/9102.20.0111
V. Chukwu
This study examined the effect of crude oil on Heligmosomoides bakeri in albino mice. A total of 35 albino mice of 5-8 weeks were used for this study. The mice were randomly divided into five groups (A, B, C, D and E) of 5 mice each and infected with 0.13ml of H. bakeri larvae. Mice in the first two groups (A and B) were given crude oil per os at the concentrations of 0.2 and 0.05 mg/ml respectively. Those in group C were given Albendazole and group D infected but not treated. Group E was used as a naïve control group. Two other groups F and G were used for toxicity test. All mice were observed for clinical signs and symptoms of abnormality all through the study and all parameters were assessed following standard procedures for 5 weeks. Administration of crude oil per os to mice in groups A and B at the dose levels used in this study showed no significant (p>0.05) changes in PCV, body weight and fecal egg counts of the mice. There was a significant (p<0.05) difference in larval mortality test between the crude oil treated groups compared to the control group by their movement (if normal, moving or dead i.e. no observed motion within 10 mins). The Albendazole group C had the least worm burden and fecal egg count compared to the groups treated with different concentration of Crude oil. In conclusion, it may be suggested that heavily infected animals may not respond to treatment with crude oil against nematodes which discredit the unorthodox folklore medicine for parasitic treatment by some rural. Its effect on the worms appeared to be transient.
{"title":"The Effects of Bonny Light Crude Oil (Blco) on the Helminth Parasite Heligmosomoides Bakeri (Polygrus) of Mice.","authors":"V. Chukwu","doi":"10.36108/jvbs/9102.20.0111","DOIUrl":"https://doi.org/10.36108/jvbs/9102.20.0111","url":null,"abstract":"This study examined the effect of crude oil on Heligmosomoides bakeri in albino mice. A total of 35 albino mice of 5-8 weeks were used for this study. The mice were randomly divided into five groups (A, B, C, D and E) of 5 mice each and infected with 0.13ml of H. bakeri larvae. Mice in the first two groups (A and B) were given crude oil per os at the concentrations of 0.2 and 0.05 mg/ml respectively. Those in group C were given Albendazole and group D infected but not treated. Group E was used as a naïve control group. Two other groups F and G were used for toxicity test. All mice were observed for clinical signs and symptoms of abnormality all through the study and all parameters were assessed following standard procedures for 5 weeks. Administration of crude oil per os to mice in groups A and B at the dose levels used in this study showed no significant (p>0.05) changes in PCV, body weight and fecal egg counts of the mice. There was a significant (p<0.05) difference in larval mortality test between the crude oil treated groups compared to the control group by their movement (if normal, moving or dead i.e. no observed motion within 10 mins). The Albendazole group C had the least worm burden and fecal egg count compared to the groups treated with different concentration of Crude oil. In conclusion, it may be suggested that heavily infected animals may not respond to treatment with crude oil against nematodes which discredit the unorthodox folklore medicine for parasitic treatment by some rural. Its effect on the worms appeared to be transient.","PeriodicalId":17442,"journal":{"name":"Journal of Veterinary and Biomedical Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77965063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-02-01DOI: 10.36108/jvbs/9102.20.0181
B. Mofio, J. Danboyi
Physicochemical analysis of Biodiesel produced from soya bean oil was carried out in order to ascertain the quality of the biodiesel produced as regards its physical and chemical parameters which includes visual appearance, colour, cloud point, flash point, pour point, cetane index, diesel index, kinematic velocity and calorific value. The oil was extracted from 60g of soya bean seeds (Glycine max) using the soxhlet method of extraction. Biodiesel which is biodegradable was therefore produced by chemical reaction of the oil with a methoxide solution containing 7cm3 of methanol and 0.2g of Sodium Hydroxide as the catalyst through a process called transesterification. Two products were obtained, the glycerin which is denser settled at the bottom while the fatty acid methyl esters was found to be on the top. 8.4cm3 of biodiesel and 3cm3of glycerin was recovered, the yield of biodiesel was calculated to be 50.74% and then it was subjected to physicochemical analysis to ascertain its quality. The properties of biodiesel was tested and found to be in confinement with the ASTM D6751 specification for biodiesel fuels and the results obtained with test methods used for colour and appearance was established to be a yellowish and clear liquid (visual), cloud point +8OC (D5773), pour point +4OC (D5950) and acid value 0.3 mg/KOH/g (titrimetric). Based on the result for the cloud and pour point of the produced biodiesel, B100 (100% biodiesel) can be used in Nigeria with no challenge as compared to country like Russia where their temperature is extremely cold which could cause biodiesel to gel and then solidify.
{"title":"Physicochemical Analysis and Characterization of Biodiesel Produced From Soya Bean (Glycine max) Oil.","authors":"B. Mofio, J. Danboyi","doi":"10.36108/jvbs/9102.20.0181","DOIUrl":"https://doi.org/10.36108/jvbs/9102.20.0181","url":null,"abstract":"Physicochemical analysis of Biodiesel produced from soya bean oil was carried out in order to ascertain the quality of the biodiesel produced as regards its physical and chemical parameters which includes visual appearance, colour, cloud point, flash point, pour point, cetane index, diesel index, kinematic velocity and calorific value. The oil was extracted from 60g of soya bean seeds (Glycine max) using the soxhlet method of extraction. Biodiesel which is biodegradable was therefore produced by chemical reaction of the oil with a methoxide solution containing 7cm3 of methanol and 0.2g of Sodium Hydroxide as the catalyst through a process called transesterification. Two products were obtained, the glycerin which is denser settled at the bottom while the fatty acid methyl esters was found to be on the top. 8.4cm3 of biodiesel and 3cm3of glycerin was recovered, the yield of biodiesel was calculated to be 50.74% and then it was subjected to physicochemical analysis to ascertain its quality. The properties of biodiesel was tested and found to be in confinement with the ASTM D6751 specification for biodiesel fuels and the results obtained with test methods used for colour and appearance was established to be a yellowish and clear liquid (visual), cloud point +8OC (D5773), pour point +4OC (D5950) and acid value 0.3 mg/KOH/g (titrimetric). Based on the result for the cloud and pour point of the produced biodiesel, B100 (100% biodiesel) can be used in Nigeria with no challenge as compared to country like Russia where their temperature is extremely cold which could cause biodiesel to gel and then solidify.","PeriodicalId":17442,"journal":{"name":"Journal of Veterinary and Biomedical Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83541853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-02-01DOI: 10.36108/jvbs/9102.20.0180
S. Adamu
Q-fever is a zoonotic disease caused by Coxiella burnetii, an intracellular Gram-negative bacterium. A cross sectional epidemiological study was conducted to determine the seroprevalence of Q-fever in flocks of sheep in Yobe State, Nigeria. Simple random sampling technique was used in selecting the animals. A total of 420 blood samples from sheep of various ages were tested from April, 2018 to July, 2018 for Q-fever using indirect enzyme linked immunosorbent assay (ELISA). Out of the 420 sera tested 49 (11.7%) were seeropositive for Q fever. Of the 315 females sheep tested, 39 (12.4%) were seropositive and out of 105 male sheep tested, 10 (9.5%) were seropositive. There was no significant association (p>0.05) between the sex of sheep tested for Q fever. The seroprevalence was higher in animals greater than 2 years 41 (12.1%) than in animals less than 2 years 8 (9.8%). There were no significant associations between age and infection with Q-fever even though that animal greater than 2 years showed higher prevalence than those less than 2 years. The highest seroprevalence 22 (12.3%) was recorded in Yankasa sheep, and the least seroprevalence 8(9.9%) was recorded in Uda. The seroprevalence of 19 (13.6%), 16 (11.4%) and 14 (10.0%) were recorded in Damaturu, Potiskum and Gashua zones respectively. There was no significant association (p>0.05) between the breed and location of the animals tested for Q fever infection. This study concludes that Q-fever is endemic in sheep in Yobe State. Enlightenment campaign is recommended to educate the livestock farmers, herders and the general public on the dangers of Q-fever infection. There is need for large scale epidemiological investigation of the disease in other livestock farms in the state.
{"title":"Epidemiology of Q – Fever in Flocks of Sheep in Yobe State, Nigeria","authors":"S. Adamu","doi":"10.36108/jvbs/9102.20.0180","DOIUrl":"https://doi.org/10.36108/jvbs/9102.20.0180","url":null,"abstract":"Q-fever is a zoonotic disease caused by Coxiella burnetii, an intracellular Gram-negative bacterium. A cross sectional epidemiological study was conducted to determine the seroprevalence of Q-fever in flocks of sheep in Yobe State, Nigeria. Simple random sampling technique was used in selecting the animals. A total of 420 blood samples from sheep of various ages were tested from April, 2018 to July, 2018 for Q-fever using indirect enzyme linked immunosorbent assay (ELISA). Out of the 420 sera tested 49 (11.7%) were seeropositive for Q fever. Of the 315 females sheep tested, 39 (12.4%) were seropositive and out of 105 male sheep tested, 10 (9.5%) were seropositive. There was no significant association (p>0.05) between the sex of sheep tested for Q fever. The seroprevalence was higher in animals greater than 2 years 41 (12.1%) than in animals less than 2 years 8 (9.8%). There were no significant associations between age and infection with Q-fever even though that animal greater than 2 years showed higher prevalence than those less than 2 years. The highest seroprevalence 22 (12.3%) was recorded in Yankasa sheep, and the least seroprevalence 8(9.9%) was recorded in Uda. The seroprevalence of 19 (13.6%), 16 (11.4%) and 14 (10.0%) were recorded in Damaturu, Potiskum and Gashua zones respectively. There was no significant association (p>0.05) between the breed and location of the animals tested for Q fever infection. This study concludes that Q-fever is endemic in sheep in Yobe State. Enlightenment campaign is recommended to educate the livestock farmers, herders and the general public on the dangers of Q-fever infection. There is need for large scale epidemiological investigation of the disease in other livestock farms in the state.","PeriodicalId":17442,"journal":{"name":"Journal of Veterinary and Biomedical Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76604572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-02-01DOI: 10.36108/jvbs/9102.20.0101
O. Salami
Ascariasis is a neglected tropical disease prevalent in areas with poor hygiene and low standard of living in tropical countries. Hyptis suaveolens is been used traditionally around the world for various ailments. The aim of the study was to evaluate the anthelminthic activity of the methanolic leaf extract of the plant on the three life cycle stages of Ascaris suum synonymous to Ascaris lumbricoides. The phytochemical analysis of the extract was evaluated. The in-vitro anthelminthic activity of the methanolic leaf extract of Hyptis suaveolens on the egg hatchability and larva inhibition of Ascaris suum were assessed using Bizimenyara inhibition method, where 100 eggs were counted and incubated in 5 different plate-wells containing positive control, negative control and 3 concentrations (50, 70 and 100 mg/ml) of extract. The plate wells were brought out after incubation and content examined under the microscope. The effect of the methanolic leaf extract and pyrantel pamoate on cuticle peeling, motility and mortality were evaluated using adult worms. The egg hatchability and larval development after incubation for 48 h and 21 days significantly (p<0.05 for both) inhibited with 98% unhatched eggs and 95% undeveloped larva of A. suum respectively. There was significant (p<0.05) visible peeling of cuticles, reduced motility and mortality of the adult worms after 30, 12 and 80 minutes respectively. It can be concluded that H. suaveolens has anthelminthic activity on A. suum which may be attributed to some of the phytochemical constituents.
{"title":"In vitro Anthelminthic Activity of Crude Methanolic Leaf Extract of Hyptis suaveolens (L.) Poit (Bush mint) on Ascaris suum.","authors":"O. Salami","doi":"10.36108/jvbs/9102.20.0101","DOIUrl":"https://doi.org/10.36108/jvbs/9102.20.0101","url":null,"abstract":"Ascariasis is a neglected tropical disease prevalent in areas with poor hygiene and low standard of living in tropical countries. Hyptis suaveolens is been used traditionally around the world for various ailments. The aim of the study was to evaluate the anthelminthic activity of the methanolic leaf extract of the plant on the three life cycle stages of Ascaris suum synonymous to Ascaris lumbricoides. The phytochemical analysis of the extract was evaluated. The in-vitro anthelminthic activity of the methanolic leaf extract of Hyptis suaveolens on the egg hatchability and larva inhibition of Ascaris suum were assessed using Bizimenyara inhibition method, where 100 eggs were counted and incubated in 5 different plate-wells containing positive control, negative control and 3 concentrations (50, 70 and 100 mg/ml) of extract. The plate wells were brought out after incubation and content examined under the microscope. The effect of the methanolic leaf extract and pyrantel pamoate on cuticle peeling, motility and mortality were evaluated using adult worms. The egg hatchability and larval development after incubation for 48 h and 21 days significantly (p<0.05 for both) inhibited with 98% unhatched eggs and 95% undeveloped larva of A. suum respectively. There was significant (p<0.05) visible peeling of cuticles, reduced motility and mortality of the adult worms after 30, 12 and 80 minutes respectively. It can be concluded that H. suaveolens has anthelminthic activity on A. suum which may be attributed to some of the phytochemical constituents.","PeriodicalId":17442,"journal":{"name":"Journal of Veterinary and Biomedical Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75855693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-02-01DOI: 10.36108/jvbs/9102.20.0151
M. Adam
Tiger nut is a small tuberous rhizome with medicinal and antioxidant properties. The present study was conducted to investigate the ameliorative effect of the methanol extract of tiger nut against the oxidative stress induced by lead acetate poisoning in Red Sokoto goat (RSG). Twelve adults Red Sokoto goat (16.50 ± 1.41 to 21.92 ± 0.97 kg) were grouped into four. Group I was administered with equivalent volume of distilled water. Group II was administered with lead acetate (200 mg/kg) only. Group III was administered with methanol extract of tiger nut (METN) (150 mg/kg) and lead acetate (200 mg/kg). Group IV was administered METN (200 mg/kg) only. The serum oxidative stress changes in the different experimental groups were determined by commercial kits. The distilled water group showed no toxic response. The lead acetate (200 mg/kg) group showed decreased activity levels of glutathione, superoxide dismutase (SOD) and catalase (CAT). The co-treatment groups with lead acetate and METN showed an increase in serum glutathione peroxidase (GPx) activity, CAT and SOD. Additionally, there was a decreased MDA concentration in the same group. The group administered with METN only showed slight changes in the activities of oxidative stress makers. In conclusion, Oxidative stress biomarkers such as glutathione peroxidases, superoxide dismutase, catalase and malondialdehyde were altered in a manner indicative of oxidative stress following lead acetate exposure and ameliorated to some extent by methanolic extract of tiger nut.
{"title":"Ameliorative Effect of Methanol Extract of Tiger Nut (Cyperus esculentus) On Oxidative Stress Induced By Lead Poisoning In Red Sokoto Goat","authors":"M. Adam","doi":"10.36108/jvbs/9102.20.0151","DOIUrl":"https://doi.org/10.36108/jvbs/9102.20.0151","url":null,"abstract":"Tiger nut is a small tuberous rhizome with medicinal and antioxidant properties. The present study was conducted to investigate the ameliorative effect of the methanol extract of tiger nut against the oxidative stress induced by lead acetate poisoning in Red Sokoto goat (RSG). Twelve adults Red Sokoto goat (16.50 ± 1.41 to 21.92 ± 0.97 kg) were grouped into four. Group I was administered with equivalent volume of distilled water. Group II was administered with lead acetate (200 mg/kg) only. Group III was administered with methanol extract of tiger nut (METN) (150 mg/kg) and lead acetate (200 mg/kg). Group IV was administered METN (200 mg/kg) only. The serum oxidative stress changes in the different experimental groups were determined by commercial kits. The distilled water group showed no toxic response. The lead acetate (200 mg/kg) group showed decreased activity levels of glutathione, superoxide dismutase (SOD) and catalase (CAT). The co-treatment groups with lead acetate and METN showed an increase in serum glutathione peroxidase (GPx) activity, CAT and SOD. Additionally, there was a decreased MDA concentration in the same group. The group administered with METN only showed slight changes in the activities of oxidative stress makers. In conclusion, Oxidative stress biomarkers such as glutathione peroxidases, superoxide dismutase, catalase and malondialdehyde were altered in a manner indicative of oxidative stress following lead acetate exposure and ameliorated to some extent by methanolic extract of tiger nut.","PeriodicalId":17442,"journal":{"name":"Journal of Veterinary and Biomedical Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82582462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-02-01DOI: 10.36108/jvbs/9102.20.0150
O. Nwankudu
Follicle Stimulating Hormone (FSH) is a polypeptide hormone secreted by the cells of the anterior pituitary whose primary function is stimulation of ovarian follicle to grow and mature in females. Additionally, FSH stimulates the granulosa cells in the ovarian follicle to synthesize aromatase which converts androgen produced by the thecal cells to estradiol. Estradiol in the blood primes the hypothalamus to produce stronger pulses of Gonadotropin Releasing Hormone (GnRH) leading to secretion of Luteinizing hormone (LH). Then, LH causes ovulation and the developmentof corpus luteum. But, in the males, FSH stimulates the Sertoli cells to secret Androgen Binding Protein (ABP) which concentrates local testosterone leading to stimulation of spermatogenesis. However, FSH has been identified in many angiogenic vasculature of many tumors. The review tries to bring out FSH in reproduction and pathology as well as reveal certain solutions which may be useful in infertility and oncogenic therapy.
{"title":"A Review of the Physiopathological Role of Follicle Stimulating Hormone (FSH) in Reproduction and in Vascularization of Tumors","authors":"O. Nwankudu","doi":"10.36108/jvbs/9102.20.0150","DOIUrl":"https://doi.org/10.36108/jvbs/9102.20.0150","url":null,"abstract":"Follicle Stimulating Hormone (FSH) is a polypeptide hormone secreted by the cells of the anterior pituitary whose primary function is stimulation of ovarian follicle to grow and mature in females. Additionally, FSH stimulates the granulosa cells in the ovarian follicle to synthesize aromatase which converts androgen produced by the thecal cells to estradiol. Estradiol in the blood primes the hypothalamus to produce stronger pulses of Gonadotropin Releasing Hormone (GnRH) leading to secretion of Luteinizing hormone (LH). Then, LH causes ovulation and the developmentof corpus luteum. But, in the males, FSH stimulates the Sertoli cells to secret Androgen Binding Protein (ABP) which concentrates local testosterone leading to stimulation of spermatogenesis. However, FSH has been identified in many angiogenic vasculature of many tumors. The review tries to bring out FSH in reproduction and pathology as well as reveal certain solutions which may be useful in infertility and oncogenic therapy.","PeriodicalId":17442,"journal":{"name":"Journal of Veterinary and Biomedical Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85903173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-02-01DOI: 10.36108/jvbs/9102.20.0120
Bolajoko Muhammad-Bashir, Y. Atuman
Despite availability of safer and cost-effective treatments and vaccines, rabies is still considered among the most fatal viral zoonotic disease worldwide. The epidemiology of rabies is directly associated with the ecology of the reservoirs which needs better understanding to underpin appropriate control measures. There are only few reported attempts made towards understanding the ecology of dog and rabies cases in Nigeria. Although dogs are tolerated and kept in Bauchi State, the risk factors associated with dog ownership in the state, remain to be studied. This study is aimed at determining the risk factors associated with dog ownership in Bauchi state and to evaluate the degree with which the presence or absence of the identified factors can increase or decrease risk of rabies cases in the state. A questionnaire-based survey was conducted amongst dog owners across the state. Basic descriptive analyses were carried out in Microsoft Excel 2016 and IBM SPSS 21 was used to determine the relative risk (RR) of rabies occurrence in households across the state when exposed to each of the identified risk factors where P-value was set at 0.05. A total of 80 questionnaires were completed and received from the respondents. The calculated RR, revealed that the practice of extensive system of management increases the risk of canine rabies amongst dogs of the state by 80% and knowledge of dog owners about canine rabies reduces the risk of canine rabies by 27%. This study confirmed that dogs are owned and tolerated but poorly managed under extensive management system in Bauchi state with increasing risks of rabies spread due to low vaccination coverage and lack of practical application of knowledge on the dangers of rabies amongst the populace. The public health implication of this situation and the need for concerted efforts for sustainable control of rabies in the state is discussed
{"title":"Rabies Ecology in Bauchi State, Nigeria","authors":"Bolajoko Muhammad-Bashir, Y. Atuman","doi":"10.36108/jvbs/9102.20.0120","DOIUrl":"https://doi.org/10.36108/jvbs/9102.20.0120","url":null,"abstract":"Despite availability of safer and cost-effective treatments and vaccines, rabies is still considered among the most fatal viral zoonotic disease worldwide. The epidemiology of rabies is directly associated with the ecology of the reservoirs which needs better understanding to underpin appropriate control measures. There are only few reported attempts made towards understanding the ecology of dog and rabies cases in Nigeria. Although dogs are tolerated and kept in Bauchi State, the risk factors associated with dog ownership in the state, remain to be studied. This study is aimed at determining the risk factors associated with dog ownership in Bauchi state and to evaluate the degree with which the presence or absence of the identified factors can increase or decrease risk of rabies cases in the state. A questionnaire-based survey was conducted amongst dog owners across the state. Basic descriptive analyses were carried out in Microsoft Excel 2016 and IBM SPSS 21 was used to determine the relative risk (RR) of rabies occurrence in households across the state when exposed to each of the identified risk factors where P-value was set at 0.05. A total of 80 questionnaires were completed and received from the respondents. The calculated RR, revealed that the practice of extensive system of management increases the risk of canine rabies amongst dogs of the state by 80% and knowledge of dog owners about canine rabies reduces the risk of canine rabies by 27%. This study confirmed that dogs are owned and tolerated but poorly managed under extensive management system in Bauchi state with increasing risks of rabies spread due to low vaccination coverage and lack of practical application of knowledge on the dangers of rabies amongst the populace. The public health implication of this situation and the need for concerted efforts for sustainable control of rabies in the state is discussed","PeriodicalId":17442,"journal":{"name":"Journal of Veterinary and Biomedical Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86643844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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