Journal of Traditional and Complementary Medicine最新文献

{"title":"Integration of transcriptomics and metabolomics to study the mechanism of Siwei Huangqi powder in Tibetan medicine for protecting against high-altitude hypoxic brain injuries","authors":"Wangjie Cao ,&nbsp;Jiawang Guo ,&nbsp;Nengxian Zhang ,&nbsp;Xinjue Zhang ,&nbsp;Congyi Li ,&nbsp;Yong Huang ,&nbsp;Jianzheng He ,&nbsp;Yongqi Liu ,&nbsp;Hongxia Gong ,&nbsp;Yun Su","doi":"10.1016/j.jtcme.2024.11.002","DOIUrl":"10.1016/j.jtcme.2024.11.002","url":null,"abstract":"<div><h3>Background and aim</h3><div>Siwei Huangqi powder (SWHQP) is a folk medicine that is extensively used in Tibetan medicine. It is widely employed at the medical institutions of various monasteries in the Tibetan region and is highly recommended by esteemed folk practitioners. It is mainly used in clinical practice for high-altitude diseases caused by an imbalance of the three bases of Tibetan medicine, namely, Long, Chiba, and Peigen, which leads to disorders of qi and blood circulation. This study aimed to investigate the potential molecular mechanism underlying the therapeutic and preventive effects of SWHQP in a high-altitude hypoxia brain injury (HHBI) rat model.</div></div><div><h3>Methods</h3><div>An HCP-III experimental animal low-pressure simulation chamber was used to simulate high-altitude hypoxic environmental exposure in rats to establish an HHBI model. The severity of brain injury, including the brain wet/dry (W/D) ratio and H&amp;E staining, was evaluated through a series of assessments. Transcriptomic and metabolomic analyses were performed to identify gene expression changes and metabolite alterations in brain tissue. Western blotting and immunofluorescence were used to assess the relative expression of proteins involved in the phosphatidylinositol 3-kinase (PI3K)/protein kinase B (AKT)/nuclear factor-kappa B p65 (NF-kB p65) pathway. The expression levels of genes related to the PI3K/AKT/NF-κB p65 signaling pathway were detected via real-time PCR. Enzyme-linked immunosorbent assays (ELISAs) were conducted to measure the serum levels of proinflammatory cytokines such as tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and interleukin-1β (IL-1β).</div></div><div><h3>Results</h3><div>SWHQP significantly improved brain function, reduced the wet/dry ratio, and alleviated brain tissue damage. Transcriptomic and metabolomic analyses revealed that the PI3K/AKT/NF-κB signaling pathway was significantly upregulated in the HHM group and that the expression of inflammatory factors related to amino acid metabolism and lipid metabolism was increased. A previous study also revealed that SWHQP inhibited the activation of the PI3K/AKT/NF-κB p65 signaling pathway in brain tissue, reducing the release of the downstream proinflammatory cytokines IL-6, IL-1β, and TNF-α.</div></div><div><h3>Conclusion</h3><div>The therapeutic effect of SWHQP on brain injury in HHBI rats is attributed to its ability to regulate inflammation-related amino acid synthesis and lipid metabolism and modulate inflammation-related pathways. These findings provide a robust research foundation for the potential clinical application of SWHQP in Tibetan medicine.</div></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"15 7","pages":"Pages 745-759"},"PeriodicalIF":3.0,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145384180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antiosteoporotic activity of nodakenetin, a coumarin compound from Angelica decursiva, by activation of the Wnt/β-catenin signaling pathway","authors":"Eun Ju Jeong ,&nbsp;Jayoung Song ,&nbsp;Hyen Joo Park ,&nbsp;Jae Sue Choi ,&nbsp;Sang Kook Lee","doi":"10.1016/j.jtcme.2024.11.001","DOIUrl":"10.1016/j.jtcme.2024.11.001","url":null,"abstract":"<div><div><em>Angelica decursiva</em> (Umbelliferae) is a medicinal plant widely used to treat colds, coughs and fevers in Korea, Japan, and mainland China. The anti-inflammatory activity of nodakenetin, a furano-coumarin compound from <em>A. decursiva</em>, has been reported, although, the antiosteoporotic activity remains unknown. This study sought to investigate the antiosteoporotic activity and precise mechanism of action of nodakenetin in <em>vitro</em> cell culture and <em>in vivo</em> bone remodeling models. The transcriptional activity of nodakenetin on the Wnt signaling pathway was assessed using the TOPflash/FOPflash assay. The effect of nodakenetin on the osteoblast differentiation was measured using Alizarin red staining and alkaline phosphatase (ALP) activity. Western blotting and real-time RT-PCR were used to assess the effect of nodakenetin on the expression of markers related to Wnt/β-catenin pathway and osteoblast differentiation. The <em>in vivo</em> antiosteoporotic activity of nodakenetin was assessed using an ovariectomized (OVX)-induced bone loss mouse model. Nodakenetin activated the Wnt/β-catenin pathway through regulation of DKK1, β-catenin and other target proteins of the Wnt/β-catenin pathway in HEK293 and MC3T3-E1 cells. Nodakenetin induced the differentiation of MC3T3-E1 cells as shown by enhanced Alizarin red staining and ALP activity. Induction of osteoblast differentiation was related to upregulated expression of bone formation biomarkers such as bone morphogenic proteins and Runx2. Oral administration of nodakenetin in the OVX mouse model effectively protected the deterioration of bone microstructure in OVX mice. Nodakenetin exhibits antiosteoporotic activity in <em>vitro</em> and <em>in vivo</em> through the activation of the Wnt/β-catenin pathway and subsequent induction of osteoblast differentiation.</div></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"15 7","pages":"Pages 736-744"},"PeriodicalIF":3.0,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145384179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electroacupuncture improves cardiac function in hyperlipidemic rats by enhancing efferocytosis in myocardial macrophages via the PPARγ-LXRα-MerTK pathway","authors":"Yong-Li Han ,&nbsp;Shu-Wen Jin ,&nbsp;Xiao-Li Pan ,&nbsp;Hong-Xing Zhang","doi":"10.1016/j.jtcme.2024.10.003","DOIUrl":"10.1016/j.jtcme.2024.10.003","url":null,"abstract":"<div><h3>Background and aim</h3><div>Electroacupuncture (EA) is an effective treatment for hyperlipidemia because it decreases the protein expression of peroxisome proliferator-activated receptor γ (PPARγ) and liver X receptor alpha (LXRα). Lowering blood lipids can reverse cardiac insufficiency, but the underlying mechanisms remain unclear. We aimed to explore whether EA reduces blood lipid levels and restores cardiac function in hyperlipidemic rats and to elucidate the underlying mechanism involved.</div></div><div><h3>Procedures</h3><div>A hyperlipidemic rat model was established by providing the animals with a high-fat diet (HFD). Animals were divided into normal control diet (NC), HFD, PPARγ siRNA, EA, and EA + PPARγ siRNA groups. H&amp;E staining was used to observe the histopathology of the heart and liver. Transmission electron microscopy and oil red O staining were used to detect the distribution of myocardial lipid droplets and the efferocytosis of macrophages. Double immunofluorescence was used to detect the expression of ATP binding cassette transport A1 (ABCA1), ATP binding cassette transport G1 (ABCG1), PPARγ, LXR, and MER proto-oncogene tyrosine kinase (MerTK) in cardiac macrophages. The MerTK−/− rats and littermate wild-type rats were divided into NC, HFD, and EA groups. The efferocytosis rate of myocardial macrophages in different groups was detected using TUNEL and Annexin V-FITC/PI double staining.</div></div><div><h3>Results and conclusion</h3><div>EA had positive effects on the blood lipid profile, cardiac function, and heart and liver weights; reduced fat deposition in cardiomyocytes and hepatocytes; promoted reverse cholesterol transport; and enhanced macrophage efferocytosis in HFD-fed rats. This effect was blocked by PPARγ siRNA. The protein expression of the PPARγ-LXRα-MerTK pathway was also increased by EA treatment. However, under MerTK−/− conditions, the beneficial effects of EA on efferocytosis in myocardial macrophages were blocked. EA reversed ectopic lipid deposition in the liver and heart, increased efferocytosis in myocardial macrophages, improved hyperlipidemia, and ameliorated cardiac dysfunction in HFD-fed rats through the PPARγ-LXRα-MerTK pathway.</div></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"15 4","pages":"Pages 423-433"},"PeriodicalIF":3.3,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144491432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Parthenolide alleviates doxorubicin-induced cardiotoxicity via regulation of Cyp1a1 and Nppa and suppression of NLRP3 inflammasome","authors":"Yuanying Yang ,&nbsp;Shanshan Wei ,&nbsp;Sa Liu ,&nbsp;Suifen Xie ,&nbsp;Wei Xiao ,&nbsp;Jian Liu ,&nbsp;Ning Xie ,&nbsp;Wenqun Li ,&nbsp;Bikui Zhang","doi":"10.1016/j.jtcme.2024.10.004","DOIUrl":"10.1016/j.jtcme.2024.10.004","url":null,"abstract":"<div><h3>Background and aim</h3><div>Doxorubicin (Dox) has limited clinical use due to its multiple adverse reactions, typically severe cardiotoxicity. Parthenolide (PTL) is the main active ingredient extracted from the buds of <em>Tanacetum balsamita</em> and exhibits diverse pharmacological properties. However, the cardioprotective effects and underlying mechanisms of PTL against Dox-induced cardiotoxicity (DIC) need to be fully investigated. Herein, this study was designed to explore the protective mechanism of PTL against DIC.</div></div><div><h3>Experimental procedure</h3><div>A stable cardiotoxicity model was established in H9c2 cells (1 μM for 24 h) and C57BL/6 J mice (15 mg/kg). RNA sequencing was used to identify key genes mediating the protection of PTL against DIC. The key genes and mechanism of PTL against DIC were comprehensively examined by transcriptomic technologies and experimental validation.</div></div><div><h3>Results and conclusion</h3><div>A combination administration of PTL effectively inhibited Dox-induced cytotoxicity as well as cardiomyocyte apoptosis in H9c2 cells. PTL also exerts a protective effect on Dox-induced cardiac injury by improving myocardial function, histological morphological changes, and myocardial apoptosis in Dox-treated mice. Subsequently, we utilized the transcriptomic approach and validated the results by RT-qPCR, confirming that <em>Cyp1a1</em> and <em>Nppa</em> were the key genes in PTL against DIC. PTL could also protect from DIC via the suppression of the NLRP3 inflammasome activation and subsequent secretion of IL-1β and Caspase1. Our study confirmed that PTL treatment attenuated DIC in mice and H9c2 cells via regulation of <em>Nppa</em> and <em>Cyp1a1</em> and the suppression of the NLRP3 inflammasome activation and subsequent secretion of pro-inflammatory cytokines.</div></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"15 7","pages":"Pages 726-735"},"PeriodicalIF":3.0,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145384178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-inflammatory and antinociceptive effects of Aloysia gratissima leaves essential oil: An in vivo study","authors":"Maryelen A. Souza ,&nbsp;Ketelin Kunh ,&nbsp;Otávio Sanaiotto ,&nbsp;Ana C. Provinelli ,&nbsp;Mayara Barufke ,&nbsp;Monica S.Z. Schindler ,&nbsp;Samara Cristina Mazon ,&nbsp;J. Vladimir Oliveira ,&nbsp;Indiara Brusco ,&nbsp;Jaqueline Scapinello ,&nbsp;Jacir Dal Magro ,&nbsp;Liz G. Müller","doi":"10.1016/j.jtcme.2024.10.002","DOIUrl":"10.1016/j.jtcme.2024.10.002","url":null,"abstract":"<div><h3>Background and aim</h3><div><em>Aloysia gratissima</em> is used in popular medicine as an analgesic and sedative. However, studies on its biological activities are still scarce. Therefore, this work aimed to evaluate the antinociceptive and anti-inflammatory effect of <em>A. gratissima</em> leaves essential oil (OAG) in mice.</div></div><div><h3>Experimental procedure</h3><div>The OAG was obtained by hydrodistillation and its composition was determined by gas chromatography-mass spectrometry. The sesquiterpenes pinocamphone, <em>β-</em>pinene, and guaiol were the major constituents of OAG. The effect of OAG (1, 10, and 30 mg/kg, p.o.) was assessed by pre-treating male mice 1h before the formalin assay, carrageenan-induced peritonitis test, or the paw edema induced by arachidonic acid (AA). The acute toxicity of OAG (2000 mg/kg, p.o.) was also investigated.</div></div><div><h3>Results and conclusion</h3><div>The minimum effective dose of OAG in the formalin test was 1 mg/kg. This dose did not affect the locomotor activity of mice. The OAG decreased the number of leukocytes/mm³ in the peritoneal exudate of mice and reduced paw edema 45 min after the arachidonic acid injection. OAG treatment elicited a reduction in NPSH levels in the paw tissue and increased NPSH levels in mice blood plasma and did not cause mice mortality. The OAG is devoid of acute toxicity and shows anti-inflammatory activity, which can be related to the presence of pinocamphone, guaiol, and <em>β-</em>pinene and is mediated, at least in part, by mechanisms that involve the participation of NPSH.</div></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"15 7","pages":"Pages 714-725"},"PeriodicalIF":3.0,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145384169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isoliquiritigenin: A natural compound with a promising role in inhibiting breast cancer lung metastasis","authors":"Kumar Ganesan ,&nbsp;Cong Xu ,&nbsp;Bing Du ,&nbsp;Jianhua Che ,&nbsp;Fei Gao ,&nbsp;Chuan Zheng ,&nbsp;Jianping Chen","doi":"10.1016/j.jtcme.2024.10.001","DOIUrl":"10.1016/j.jtcme.2024.10.001","url":null,"abstract":"<div><h3>Background and aim</h3><div>Isoliquiritigenin (ISL) is a dietary bioactive compound that is derived from the roots of licorice and various other plants. Accumulating evidence suggests that ISL has noteworthy anticancer activity in a variety of cancer cells, but it is currently unknown how ISL affects the invasion and migration of breast cancer (BC) cells. Hence, the purpose of this study to determine the inhibitory effect of ISL on cell invasion, migration, and metastasis of BC cells in 4T1-treated lung metastatic animal models.</div></div><div><h3>Experimental procedure</h3><div>ISL was found to inhibit BC lung metastasis, as confirmed by MTT assay, clonogenic assay, wound healing assay, transwell assay, histological analysis, Western blot and bioluminescence assay in the xenograft model.</div></div><div><h3>Results and conclusion</h3><div>The in vitro studies revealed that ISL treatment significantly prevented BC cell invasion and migration for 24 h. ISL decreased the protein expressions of PI3K, Akt, p-Akt, mTOR, p-mTOR, matrix metalloproteinase (MMP)-2, MMP-9, and N-cadherin, thereby inhibiting BC cell invasion. In addition, ISL decreased MMP-2/9 and N-cadherin expression in lung tissues and reduced mouse 4T1 BC cell lung metastasis. Based on these findings, it suggests that ISL inhibits cell migration and invasion in BC cells by inhibiting the PI3K/Akt signaling pathway and decreasing the activation of MMP-2 and MMP-9 expression. Therefore, ISL may be useful in managing BC invasion and metastasis.</div></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"15 7","pages":"Pages 702-713"},"PeriodicalIF":3.0,"publicationDate":"2024-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145384254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated lipidomics and network pharmacology analysis to determine how Gu Fu Sheng Capsule improves lipid metabolism in rats with steroid-induced osteonecrosis of the femoral head","authors":"Yue Li ,&nbsp;Ying Liu ,&nbsp;Yingchun Li ,&nbsp;Yang Cao ,&nbsp;Hui Zhang ,&nbsp;Puwei Yuan ,&nbsp;Bo Dong ,&nbsp;Li Shen","doi":"10.1016/j.jtcme.2024.09.005","DOIUrl":"10.1016/j.jtcme.2024.09.005","url":null,"abstract":"<div><h3>Background</h3><div>Steroid-induced osteonecrosis of the femoral head (SONFH) is a prevalent refractory condition in orthopedics, often causing high disability rates. Gu Fu Sheng Capsule (GFSC) is commonly used to treat SONFH during the early and intermediate stages. However, the precise underlying mechanism of action of GFSC remains elusive.</div></div><div><h3>Objective</h3><div>To elucidate the mechanism through which GFSC improves lipid metabolism in rats with SONFH.</div></div><div><h3>Methods</h3><div>A rat model of SONFH was established by administering methylprednisolone and lipopolysaccharide. Micro-computed tomography assessed femoral head effects, while hematoxylin–eosin staining examined femoral head tissues. Pathological changes were evaluated using an automated biochemical analyzer to measure changes in serum lipid levels. Besides, quantitative lipidomics and network pharmacology were used to determine how GFSC impacts SONFH. qRT-PCR and Western blotting were performed to assess mRNA and protein expression levels of key targets.</div></div><div><h3>Results</h3><div>GFSC can enhance bone density and prevents surface collapse of the femoral head, improve hollow bone lacuna density, reduce adipocyte infiltration, minimize osteocyte cavities, regulate the serum lipid levels and enhance lipid metabolism in SONFH rats. Moreover, integrated lipidomics and network pharmacology suggested that GFSC affects lipid metabolism-related genes by regulating nine targets, thereby influencing four metabolites and two metabolic pathways, which may mainly be due to components in GFSC such as quercetin, tanshinone iia, berberine. Western blotting and qRT-PCR data highlighted that GFSC improves lipid metabolism by regulating TP53, HSP90AA1, and ESR1.</div></div><div><h3>Conclusion</h3><div>GFSC effectively retards SONFH progression, potentially attributed to its ability to regulate lipid metabolism.</div></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"15 7","pages":"Pages 687-701"},"PeriodicalIF":3.0,"publicationDate":"2024-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145384253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Curcumol promotes ferroptosis of colon cancer by targeting the ubiquitination and degradation of GPX4","authors":"Wuxia Zhao ,&nbsp;Qiuying Yan ,&nbsp;Lianfang Liu ,&nbsp;Dahai Hou ,&nbsp;Dongyang Xiang ,&nbsp;Dongxin Tang ,&nbsp;Liu Li ,&nbsp;Weixing Shen ,&nbsp;Weiwei Tao ,&nbsp;Haibo Cheng ,&nbsp;Dongdong Sun","doi":"10.1016/j.jtcme.2024.08.006","DOIUrl":"10.1016/j.jtcme.2024.08.006","url":null,"abstract":"<div><h3>Background and aim</h3><div>Colon cancer (CC) is one of the common malignant tumors in the digestive tract, the prognosis of CC patients has never been satisfying. A Ferrous-dependent form that regulates cell death, plays a key role in cancer development. As a core regulator of ferroptosis, GPX4 has become a potential molecular target for the development of antitumor drugs. Curcumol (Cur), a sesquiterpene natural product, it has significant anti-tumor effect. However, whether Cur mediates ferroptosis in colon cancer and its mechanism are still unclear. This study aimed to investigate the underlying mechanisms of Cur anti-tumor.</div></div><div><h3>Experimental procedure</h3><div>By investigating the Cancer Genome Atlas (TCGA) database and tissue immunofluorescence staining was also used to detect the levels of GPX4 protein in CC and matching paracancerous tissues. The anti-CC and pro-ferroptosis effects of Cur were detected in the <em>vivo</em> and <em>vitro</em> experiment. The interaction between Cur and GPX4 was predicted. In addition, the potential mechanism of Cur anti-CC was further discussed. Co-immunoprecipitation was used to confirm Cur-mediated GPX4 ubiquitination.</div></div><div><h3>Results and conclusion</h3><div>GPX4 was upregulated in CC tissue and was correlated with poor survival of patients. Cur inhibited the proliferation of CC cells, accompanied by regulating Fe²⁺ overload, reactive oxygen species (ROS) formation, malondialdehyde (MDA) production and superoxide dismutase (SOD) consumption. Furthermore, GPX4 was predicted and verified as the direct target of Cur by molecular docking and structure-based virtual prediction. Meanwhile, Cur could promote the ubiquitination-mediated degradation of GPX4, induce ferroptosis in CC cells and regulate the expression of ferroptosis-related protein FTH1 and TfR1. In addition, when GPX4 was overexpressed (GPX4-OE), the inhibitory effect of Cur on the expression of GPX4 and ferroptosis-related protein FTH1 and the promotion of TfR1 expression were abolished. Cur could inhibit CC by increasing the ubiquitination degradation level of GPX4 to induce ferroptosis in CC cells.</div></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"15 2","pages":"Pages 170-181"},"PeriodicalIF":3.3,"publicationDate":"2024-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142078010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Raddeanin A promotes the apoptosis of gastric cancer in conjunction with autophagy inhibitor Hydroxychloroquine via MAPK signaling pathway","authors":"Yuhao Teng ,&nbsp;Ying Xing ,&nbsp;Weiwei Xue ,&nbsp;Yue Hu ,&nbsp;Zirui Li ,&nbsp;Jun Qian ,&nbsp;Ruiping Wang","doi":"10.1016/j.jtcme.2024.07.004","DOIUrl":"10.1016/j.jtcme.2024.07.004","url":null,"abstract":"<div><h3>Purpose</h3><div>Gastric cancer (GC) is among the malignant cancers with the highest incidence and mortality worldwide. As GC is not very sensitive to current chemotherapy drugs, there is an urgent need to develop new effective drugs. Raddeanin A (RA) is extracted from the traditional Chinese medicine Anemone raddeana Regel, which has an anti-cancer effect. The purpose of this study was to explore the effects of RA on GC in vitro and in vivo.</div></div><div><h3>Methods</h3><div>We explored the targets of RA in GC through network pharmacology. MTT assay, flow cytometry, Western blotting, and other methods were used to detect the effects of RA on the proliferation, apoptosis, and autophagy of GC cells. After preconditioning with hydroxychloroquine (HCQ) and rapamycin, we observed the effects of RA-induced autophagy on apoptosis. We further verified the antitumor effect and safety of RA in vivo. Using SNU-1 xenograft tumor model in nude mice, tumor volume was observed and liver toxicity was observed by immunohistochemistry.</div></div><div><h3>Results</h3><div>Many cancer-related signaling pathways were visualized using Cytoscape software. Among them, the MAPK signaling pathway was one of the highest-ranked pathways. The MTT assay results suggested that RA could inhibit the proliferation of HGC-27 and SNU-1 cells effectively. Flow cytometry and Western blotting confirmed that RA could significantly induce apoptosis of HGC-27 and SNU-1 cells. Electron microscopy and Western blotting demonstrated that RA could induce autophagy of HGC-27 and SNU-1 cells. Further experiments suggested that HCQ, an autophagy inhibitor, could enhance the capacity of RA to induce apoptosis. In animal studies, we found that intraperitoneal injection of RA could effectively and safely inhibit gastric tumors.</div></div><div><h3>Conclusions</h3><div>RA significantly inhibited the proliferation and induced autophagy and apoptosis of GC cells. In combination with HCQ, RA-induced apoptosis increased in vitro. The combined application of RA and autophagy inhibitors may serve as an added approach to the treatment of GC, but the underlying mechanism needs further exploration. In vivo, it was observed that RA has a good antitumor effect without increasing liver toxicity.</div></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"15 2","pages":"Pages 161-169"},"PeriodicalIF":3.3,"publicationDate":"2024-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141842359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The chemical composition of Diwu YangGan capsule and its potential inhibitory roles on hepatocellular carcinoma by microarray-based transcriptomics","authors":"Qingxin Shi ,&nbsp;Jiangcheng He ,&nbsp;Guangya Chen ,&nbsp;Jinlin Xu ,&nbsp;Zhaoxiang Zeng ,&nbsp;Xueyan Zhao ,&nbsp;Binbin Zhao ,&nbsp;Xiang Gao ,&nbsp;Zhihua Ye ,&nbsp;Mingzhong Xiao ,&nbsp;Hanmin Li","doi":"10.1016/j.jtcme.2023.12.002","DOIUrl":"10.1016/j.jtcme.2023.12.002","url":null,"abstract":"<div><p>The Traditional Chinese Medicine compound preparation known as Diwu Yanggan capsule (DWYG) can effectively hinder the onset and progression of hepatocellular carcinoma (HCC), which is recognized worldwide as a significant contributor to fatalities associated with cancer. Nevertheless, the precise mechanisms implicated have remained ambiguous. In present study, the model of HCC was set up by the 2-acetylaminofluorene (2-AAF)/partial hepatectomy (PH) in rats. To confirm the differentially expressed genes (DEGs) identified in the microarray analysis, real-time quantitative reverse transcription PCR (qRT-PCR) was conducted. In the meantime, the liquid chromatography-quadrupole time of flight mass spectrometry (LC-QTOF-MS/MS) was employed to characterize the component profile of DWYG. Consequently, the DWYG treatment exhibited the ability to reverse 51 variation genes induced by 2-AAF/PH. Additionally, there was an overlap of 54 variation genes between the normal and model groups. Upon conducting RT-qPCR analysis, it was observed that the expression levels of all genes were increased by 2-AAF/PH and subsequently reversed after DWYG treatment. Notably, the fold change of expression levels for all genes was below 0.5, with 3 genes falling below 0.25. Moreover, an investigation was conducted to determine the signaling pathway that was activated/inhibited in the HCC group and subsequently reversed in the DWYG group. Moreover, the component profile of DWYG encompassed a comprehensive compilation of 206 compounds that were identified or characterized. The findings of this study elucidated the potential alleviative mechanisms of DWYG in the context of HCC, thereby holding significant implications for its future clinical utilization and widespread adoption.</p></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"14 4","pages":"Pages 381-390"},"PeriodicalIF":3.3,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2225411023001360/pdfft?md5=bfa76b9673e97a298fbe5a8c4cb54ab9&pid=1-s2.0-S2225411023001360-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139193418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}