The increasing awareness on the negative environmental impact of petroleum-based plastics has driven industries to explore more efficient biodegradable polymers for production of bioplastic. Polyhydroxyalkanoates (PHAs) is one of the potential biodegradable polymers to replace petroleum-based plastic. It is synthesized and accumulated as intracellular granules in microorganism. In this study, polyhydroxyalkanoates (PHAs) producing bacteria were successfully isolated from sediment collected from Kg. Batu Melintang hotspring. Isolation process was carried on Minimal Salt Medium (MSM) agar supplemented with excess glucose as a carbon source. Potential PHA producers were screened by using Nile Blue staining plate assay. Out of 144 bacterial isolates, 12 bacterial isolates which showed strong orange fluorescence under ultraviolet (UV) light (365nm) were selected for further identification by morphological characterization and biochemical analysis. Based on the result obtained, possible species for Gram positive rod shape bacteria B75 and B87 is Corynebacterium kutsceri meanwhile Gram negative rod shape bacteria A4, A12, A50, A68, B2, B13, B22, B31, B73 and C3 showed affiliation to Citrobacter sp., Enterobacter sp., Erwinia sp., Klebsiella sp., Proteus sp., Salmonella sp., Serratia sp., Shigella sp., and Yersinia sp.
{"title":"Isolation and Characterization of Polyhydroxyalkanoates (PHAs) Producers from Kg Batu Melintang hotspring","authors":"N. Lee, A. A. Rahim","doi":"10.47253/JTRSS.V8I1.482","DOIUrl":"https://doi.org/10.47253/JTRSS.V8I1.482","url":null,"abstract":"The increasing awareness on the negative environmental impact of petroleum-based plastics has driven industries to explore more efficient biodegradable polymers for production of bioplastic. Polyhydroxyalkanoates (PHAs) is one of the potential biodegradable polymers to replace petroleum-based plastic. It is synthesized and accumulated as intracellular granules in microorganism. In this study, polyhydroxyalkanoates (PHAs) producing bacteria were successfully isolated from sediment collected from Kg. Batu Melintang hotspring. Isolation process was carried on Minimal Salt Medium (MSM) agar supplemented with excess glucose as a carbon source. Potential PHA producers were screened by using Nile Blue staining plate assay. Out of 144 bacterial isolates, 12 bacterial isolates which showed strong orange fluorescence under ultraviolet (UV) light (365nm) were selected for further identification by morphological characterization and biochemical analysis. Based on the result obtained, possible species for Gram positive rod shape bacteria B75 and B87 is Corynebacterium kutsceri meanwhile Gram negative rod shape bacteria A4, A12, A50, A68, B2, B13, B22, B31, B73 and C3 showed affiliation to Citrobacter sp., Enterobacter sp., Erwinia sp., Klebsiella sp., Proteus sp., Salmonella sp., Serratia sp., Shigella sp., and Yersinia sp.","PeriodicalId":17457,"journal":{"name":"Journal of Tropical Resources and Sustainable Science (JTRSS)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74916764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Jusoh, L. Xin, Ch’ng Huck Ywih, P. S. Abdullah, N. M. Radzi, Mohd Ashraf Zainol Abidin, Muhammad Firdaus Abdul Muttalib
Beach ridges interspersed with swales (BRIS) soil is identified as one of Malaysia's problematic soil since this type of soil contains a plethora portion of sand texture that contributes to water and nutrient leaching causes infertile soil. A polybag trial was conducted to evaluate the effects of wood vinegar and rice husk biochar on soil properties and growth performances of immature kenaf. The plant was arranged in a completely randomised design with four treatments and three replications. The factors taken for the experiment were four different media compositions, i.e., T1(control), T2(BRIS soil amended with wood vinegar, WV), T3(BRIS soil amended with rice husk biochar, RHB) and T4(BRIS soil amended with wood vinegar and rice husk biochar, WV+RHB). Rice husk biochar and a combination of wood vinegar with rice husk biochar significantly improved soil properties in terms of soil moisture content, organic matter, water retention and soil porosity, as well as significantly increased the kenaf stem diameter. However, there was no significant difference in plant height, number of leaves, leaves area and chlorophyll content on different treatments medium. In conclusion, the application of rice husk biochar and wood vinegar in this study enhanced BRIS soil properties and showed a positive effect on the growth of immature kenaf.
{"title":"Effect of wood vinegar and rice husk biochar on soil properties and growth performances of immature kenaf (Hibiscus cannabinus) planted on BRIS soil","authors":"M. Jusoh, L. Xin, Ch’ng Huck Ywih, P. S. Abdullah, N. M. Radzi, Mohd Ashraf Zainol Abidin, Muhammad Firdaus Abdul Muttalib","doi":"10.47253/jtrss.v9i1.709","DOIUrl":"https://doi.org/10.47253/jtrss.v9i1.709","url":null,"abstract":"Beach ridges interspersed with swales (BRIS) soil is identified as one of Malaysia's problematic soil since this type of soil contains a plethora portion of sand texture that contributes to water and nutrient leaching causes infertile soil. A polybag trial was conducted to evaluate the effects of wood vinegar and rice husk biochar on soil properties and growth performances of immature kenaf. The plant was arranged in a completely randomised design with four treatments and three replications. The factors taken for the experiment were four different media compositions, i.e., T1(control), T2(BRIS soil amended with wood vinegar, WV), T3(BRIS soil amended with rice husk biochar, RHB) and T4(BRIS soil amended with wood vinegar and rice husk biochar, WV+RHB). Rice husk biochar and a combination of wood vinegar with rice husk biochar significantly improved soil properties in terms of soil moisture content, organic matter, water retention and soil porosity, as well as significantly increased the kenaf stem diameter. However, there was no significant difference in plant height, number of leaves, leaves area and chlorophyll content on different treatments medium. In conclusion, the application of rice husk biochar and wood vinegar in this study enhanced BRIS soil properties and showed a positive effect on the growth of immature kenaf.","PeriodicalId":17457,"journal":{"name":"Journal of Tropical Resources and Sustainable Science (JTRSS)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79584938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Suvennie Saseetheran, B. Selvam, N. R. Awang, M. Yusoff, Abdus Salam Mohamed
Particulate matter (PM) was identified as the main air pollutants in Malaysia which directly impose a treat to human health and understanding this pollutant is also crucial for its mitigation and control. This study aims to determine and characterize the PM2.5 with the influence of meteorological parameters. The PM2.5 samples were collected at Perai, Nibong Tebal and highway of Penang Bridge represented the industrial, residential, and roadside areas of Mainland Penang, respectively. The descriptive statistical analysis was conducted to understand the variations of PM2.5 concentration on the study areas, while its relationship with meteorological parameters were tested using Pearson correlation. Eleven heavy metals and three anions were determined using mass spectrometry (ICP-MS) and Ion Chromatography, respectively. Results showed that the mean concentration of PM2.5 are 10.88 ?g/m³, 31.96 ?g/m³, and 39.35 g/m³ for residential area, industrial area, and roadside, respectively. The results revealed that mean concentrations of PM2.5 (39.35 ?g/m³) in the roadside are significantly higher than NAAQG 24-h average of 35 ?g/m³. Result also suggested that meteorological parameters were lest significant in controlling the PM2.5 concentrations as only the air pressure was found to be significant with the value of correlation coefficient of 0.476. The major components such as SO42-, K and Na account for about 70-90% of the PM2.5.
{"title":"Concentration of the PM2.5 according to the sampling areas associated with the concentration of heavy metals and anions in Penang","authors":"Suvennie Saseetheran, B. Selvam, N. R. Awang, M. Yusoff, Abdus Salam Mohamed","doi":"10.47253/jtrss.v9i1.705","DOIUrl":"https://doi.org/10.47253/jtrss.v9i1.705","url":null,"abstract":"Particulate matter (PM) was identified as the main air pollutants in Malaysia which directly impose a treat to human health and understanding this pollutant is also crucial for its mitigation and control. This study aims to determine and characterize the PM2.5 with the influence of meteorological parameters. The PM2.5 samples were collected at Perai, Nibong Tebal and highway of Penang Bridge represented the industrial, residential, and roadside areas of Mainland Penang, respectively. The descriptive statistical analysis was conducted to understand the variations of PM2.5 concentration on the study areas, while its relationship with meteorological parameters were tested using Pearson correlation. Eleven heavy metals and three anions were determined using mass spectrometry (ICP-MS) and Ion Chromatography, respectively. Results showed that the mean concentration of PM2.5 are 10.88 ?g/m³, 31.96 ?g/m³, and 39.35 g/m³ for residential area, industrial area, and roadside, respectively. The results revealed that mean concentrations of PM2.5 (39.35 ?g/m³) in the roadside are significantly higher than NAAQG 24-h average of 35 ?g/m³. Result also suggested that meteorological parameters were lest significant in controlling the PM2.5 concentrations as only the air pressure was found to be significant with the value of correlation coefficient of 0.476. The major components such as SO42-, K and Na account for about 70-90% of the PM2.5.","PeriodicalId":17457,"journal":{"name":"Journal of Tropical Resources and Sustainable Science (JTRSS)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91182300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Rasat, R. Wahab, A. Shafie, Ag Ahmad Mohd Yunus, M. Yusoff
Using natural wood-fiber as reinforcement in commercial thermoplastics is gaining momentum due to its high specific properties and renewable resources. In this study, the effect of wood particle geometry size on mechanical properties of thermoplastics composite was investigated. The wood species that has been chosen is Kelempayan species (Neolamarckia cadamba) and reinforced with polypropylene using fiber geometry size of 75 and 250 ?m. Thermoplastic composites were produced from two types of ratio (30:70 and 10:90) between wood-fiber and polypropylene. Static bending and tensile strength were tested. The result showed that wood-fiber from 75 ?m geometry sizes with ratio of 30:70 between wood-fiber and polypropylene was most suitable in producing thermoplastic composites. The geometry sizes of wood particle as well as the ratio between wood-fiber and polypropylene were found to influence the mechanical properties of the thermoplastic composites.
{"title":"Effect of Wood-Fiber Geometry Size on Mechanical Properties of Wood-Fiber from Neolamarckia Cadamba Species Reinforced Polypropylene Composites","authors":"M. Rasat, R. Wahab, A. Shafie, Ag Ahmad Mohd Yunus, M. Yusoff","doi":"10.47253/jtrss.v1i1.669","DOIUrl":"https://doi.org/10.47253/jtrss.v1i1.669","url":null,"abstract":"Using natural wood-fiber as reinforcement in commercial thermoplastics is gaining momentum due to its high specific properties and renewable resources. In this study, the effect of wood particle geometry size on mechanical properties of thermoplastics composite was investigated. The wood species that has been chosen is Kelempayan species (Neolamarckia cadamba) and reinforced with polypropylene using fiber geometry size of 75 and 250 ?m. Thermoplastic composites were produced from two types of ratio (30:70 and 10:90) between wood-fiber and polypropylene. Static bending and tensile strength were tested. The result showed that wood-fiber from 75 ?m geometry sizes with ratio of 30:70 between wood-fiber and polypropylene was most suitable in producing thermoplastic composites. The geometry sizes of wood particle as well as the ratio between wood-fiber and polypropylene were found to influence the mechanical properties of the thermoplastic composites.","PeriodicalId":17457,"journal":{"name":"Journal of Tropical Resources and Sustainable Science (JTRSS)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74708153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Noor Sharina Azrin Zakari, J. J. Mohamed, Nurul Basyirah Aryani Abdul Rahman, Slina Anjang Ab Rahman, Zairul Amin Rabidin
Utilization of sandwich composite during recent year has been driven by the fact that compositematerial has ultimately high strength and stiffness by weight than any other materials. The skins ofsandwich composites technically bear most of the applied loads, however, the core materials alsoplay an important role as it functions in providing continuous support to resist the shear stress.Hence, proper selection of core materials is required to establish a sturdy sandwich compositestructure. This paper presents an experimental investigation on the sandwich structure consists offibreglass/epoxy face skins and a mahang wood core. Sandwich composite with core grain orientedin parallel and perpendicular to the flat plane direction were tested for mechanical performance intension, compression and flexure. The results indicate that sandwich composite with grain orientedin parallel direction performed better in tensile properties with strength of 201.98 MPa whereassandwich composite with perpendicular core grain produced a higher value of compressionproperties with strength of 70.11 MPa. However, no significant effect of grain orientation wasobserved in flexural strength. The strength of sandwich composite is dependent on the grainalignment of the wood core as it functions exclusively as mechanical supporting cells to supportthe wood structure.
{"title":"Effects of core grain orientation on the mechanical properties of wood sandwich composite","authors":"Noor Sharina Azrin Zakari, J. J. Mohamed, Nurul Basyirah Aryani Abdul Rahman, Slina Anjang Ab Rahman, Zairul Amin Rabidin","doi":"10.47253/jtrss.v6i1.723","DOIUrl":"https://doi.org/10.47253/jtrss.v6i1.723","url":null,"abstract":"Utilization of sandwich composite during recent year has been driven by the fact that compositematerial has ultimately high strength and stiffness by weight than any other materials. The skins ofsandwich composites technically bear most of the applied loads, however, the core materials alsoplay an important role as it functions in providing continuous support to resist the shear stress.Hence, proper selection of core materials is required to establish a sturdy sandwich compositestructure. This paper presents an experimental investigation on the sandwich structure consists offibreglass/epoxy face skins and a mahang wood core. Sandwich composite with core grain orientedin parallel and perpendicular to the flat plane direction were tested for mechanical performance intension, compression and flexure. The results indicate that sandwich composite with grain orientedin parallel direction performed better in tensile properties with strength of 201.98 MPa whereassandwich composite with perpendicular core grain produced a higher value of compressionproperties with strength of 70.11 MPa. However, no significant effect of grain orientation wasobserved in flexural strength. The strength of sandwich composite is dependent on the grainalignment of the wood core as it functions exclusively as mechanical supporting cells to supportthe wood structure.","PeriodicalId":17457,"journal":{"name":"Journal of Tropical Resources and Sustainable Science (JTRSS)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81608676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nik Muhammad Muqit Ali, Khairul Nizar Syazwan W.S.W., S. M. Al-Amsyar, H. Salleh, Hasyiya Karimah Adli
The application of natural plant-derived dyes to replace ruthenium-based material as photo-absorber in solar cells application, have been extensively studied. Several advantages such as low cost, abundant in supply, sustainability and environmentally-safe make natural materials as current favourite photo-absorber. Natural plant-derived dyes are known containing natural compounds (e.g. carotenoids, chlorophylls, anthocyanins) that have the characteristics of electronic delocalization in extended ?-orbital system involving in electronic transfer mechanism. To date, massive investigations were done to exploit this system to be used as a potential photo-absorber in solar cells. Due to this matter, the hybrid dyes from the mixture of Pandanus amaryllifolius (pandan, P) and Curcuma longa (turmeric, T) were successfully prepared and several physical characterizations were carried out to analyse the photo-absorber (sensitizer) properties. From the results obtained, the ratios of P:T was varied into 1:2, 1:4, 4:1, and 8:1. This ratio has changed the wavelength of absorbers that were slightly shifted and the indirect bandgap (Eg) also were significantly changed. With this new approach, the bandgap of the hybrid dyes as core point in modulating electrical conductivity of photo-absorber can be simply tuned. By implying two different extract dyes to form hybrid dyes, the bandgap was found decreased with higher ratio of T used. Overall results suggesting that by adjusting the ratio of hybrid dyes, the photo-absorber properties and the Eg values were differed and with slightly modification, better electrical conductivity can be expected for solar cells application.
{"title":"Bandgap tunability from synergistic mixture of Pandanus amaryllifolius and Curcuma longa as photo-absorber candidates","authors":"Nik Muhammad Muqit Ali, Khairul Nizar Syazwan W.S.W., S. M. Al-Amsyar, H. Salleh, Hasyiya Karimah Adli","doi":"10.47253/jtrss.v6i1.728","DOIUrl":"https://doi.org/10.47253/jtrss.v6i1.728","url":null,"abstract":"The application of natural plant-derived dyes to replace ruthenium-based material as photo-absorber in solar cells application, have been extensively studied. Several advantages such as low cost, abundant in supply, sustainability and environmentally-safe make natural materials as current favourite photo-absorber. Natural plant-derived dyes are known containing natural compounds (e.g. carotenoids, chlorophylls, anthocyanins) that have the characteristics of electronic delocalization in extended ?-orbital system involving in electronic transfer mechanism. To date, massive investigations were done to exploit this system to be used as a potential photo-absorber in solar cells. Due to this matter, the hybrid dyes from the mixture of Pandanus amaryllifolius (pandan, P) and Curcuma longa (turmeric, T) were successfully prepared and several physical characterizations were carried out to analyse the photo-absorber (sensitizer) properties. From the results obtained, the ratios of P:T was varied into 1:2, 1:4, 4:1, and 8:1. This ratio has changed the wavelength of absorbers that were slightly shifted and the indirect bandgap (Eg) also were significantly changed. With this new approach, the bandgap of the hybrid dyes as core point in modulating electrical conductivity of photo-absorber can be simply tuned. By implying two different extract dyes to form hybrid dyes, the bandgap was found decreased with higher ratio of T used. Overall results suggesting that by adjusting the ratio of hybrid dyes, the photo-absorber properties and the Eg values were differed and with slightly modification, better electrical conductivity can be expected for solar cells application.","PeriodicalId":17457,"journal":{"name":"Journal of Tropical Resources and Sustainable Science (JTRSS)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85961281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The study was conducted in Gua Musang, Kelantan, namely; Kuala Koh N 04° 52’ 02.2”/ E 102° 26’ 33.3” (represents pyroclastics area) and Tanah Puteh N 04° 46’ 11.9”/ E 101° 58’ 35.5” (represents limestone area). A square plot (100 x 100 m) was set-up in both locations for sampling of Araceae. The result shows diversity of Araceae in limestone (28 species ha-1 ) is higher as compared to pyroclastics area (21 species ha-1). The most abundant species in limestone are Anadendrum microstachyum, Homalomena griffithii, Rhaphidophora tenuis and Schismatoglottis brevicuspis. In pyroclastics area, the most abundant is S. calyptrata followed by, S. scortechinii, S. brevicuspis and A. microstachyum. The common species in both areas was hemiepiphytic R. mangayi. The least abundant species in limestone are Amorphophallus sp. and Homalomena Chamaecladon Supergroup. Meanwhile, Scindapsus perakensis, Homalomena Cyrtocladon Supergroup, H. pontederiifolia and Aglaonema simplex were counted as least abundant species in pyroclastics area. Geological features, topography (whether on-slope, on-ridge or edge of stream), and altitude are the most influencing factor on distribution and abundance of aroids species.
{"title":"Assessment on Diversity and Abundance of Araceae in Limestone and Pyroclastics Areas in Gua Musang, Kelantan, Malaysia","authors":"Nik Yuszrin Yusof, Z. Hamzah, F. Kayat","doi":"10.47253/jtrss.v1i1.665","DOIUrl":"https://doi.org/10.47253/jtrss.v1i1.665","url":null,"abstract":"The study was conducted in Gua Musang, Kelantan, namely; Kuala Koh N 04° 52’ 02.2”/ E 102° 26’ 33.3” (represents pyroclastics area) and Tanah Puteh N 04° 46’ 11.9”/ E 101° 58’ 35.5” (represents limestone area). A square plot (100 x 100 m) was set-up in both locations for sampling of Araceae. The result shows diversity of Araceae in limestone (28 species ha-1 ) is higher as compared to pyroclastics area (21 species ha-1). The most abundant species in limestone are Anadendrum microstachyum, Homalomena griffithii, Rhaphidophora tenuis and Schismatoglottis brevicuspis. In pyroclastics area, the most abundant is S. calyptrata followed by, S. scortechinii, S. brevicuspis and A. microstachyum. The common species in both areas was hemiepiphytic R. mangayi. The least abundant species in limestone are Amorphophallus sp. and Homalomena Chamaecladon Supergroup. Meanwhile, Scindapsus perakensis, Homalomena Cyrtocladon Supergroup, H. pontederiifolia and Aglaonema simplex were counted as least abundant species in pyroclastics area. Geological features, topography (whether on-slope, on-ridge or edge of stream), and altitude are the most influencing factor on distribution and abundance of aroids species.","PeriodicalId":17457,"journal":{"name":"Journal of Tropical Resources and Sustainable Science (JTRSS)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77998937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Antifungal activities of 6 methanol extract obtain from bark, sapwood and heartwood of Syzygium chlorantha and Hopea spp. were evaluated against the white rot fungi Pycnoporus sanguineus using a medium in which homogenised hyphae were dispersed. It is observed that Hopea spp. has antifungal activity against Pycnoporus sanguineus compared to Syzygium chlorantha. Hopea spp. bark showed the highest antifungal activity against Pycnoporus sanguineus with reaction showed very clear inhibition zone. Meanwhile, Hopea spp. sapwood showed partially clear inhibition zone. On the other hand, there are no antifungal activities occurring at heartwood of Hopea spp. and all parts of Syzygium chlorantha. The positive control glycyrrhizic acid dipotassium salt (GADS) showed clear inhibition zone from 50 ?g to 1000 ?g. The results of the sapwood and bark of Hopea spp. suggest that these extract have great potential as a source of fungi stats.
{"title":"Antifungal Activities of Methanol Extract from Syzygium chlorantha and Hopea spp. against Pycnoporus Sanguineus","authors":"M. Harmen","doi":"10.47253/jtrss.v4i2.613","DOIUrl":"https://doi.org/10.47253/jtrss.v4i2.613","url":null,"abstract":"Antifungal activities of 6 methanol extract obtain from bark, sapwood and heartwood of Syzygium chlorantha and Hopea spp. were evaluated against the white rot fungi Pycnoporus sanguineus using a medium in which homogenised hyphae were dispersed. It is observed that Hopea spp. has antifungal activity against Pycnoporus sanguineus compared to Syzygium chlorantha. Hopea spp. bark showed the highest antifungal activity against Pycnoporus sanguineus with reaction showed very clear inhibition zone. Meanwhile, Hopea spp. sapwood showed partially clear inhibition zone. On the other hand, there are no antifungal activities occurring at heartwood of Hopea spp. and all parts of Syzygium chlorantha. The positive control glycyrrhizic acid dipotassium salt (GADS) showed clear inhibition zone from 50 ?g to 1000 ?g. The results of the sapwood and bark of Hopea spp. suggest that these extract have great potential as a source of fungi stats.","PeriodicalId":17457,"journal":{"name":"Journal of Tropical Resources and Sustainable Science (JTRSS)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75417934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Lee, R. Ying, Ashraf Muhammad, Rusli N. Dini, Mohammad Sohel Rahman, H. Harun, M. K..
In this research, the rice hull and coconut hull from agriculture waste were investigated. The lignin content within the rice and coconut hull were determined before and after pre-treatment. Response Surface Methodology (RSM) used to predict the optimal condition for the treatments and Fourier Transform Infrared (FTIR) to identify the lignin content. The interaction of NaOH concentration, contact time (CT), and sample weight was investigated to optimize the lignin removal percentage (%). The correlation coefficient, R2 for a quadratic model of rice hull lignin removal was 0.8863 while for coconut hull lignin removal in the linear model was 0.7998, as well as the 2FI model was 0.8892. The optimum condition for rice hull lignin removal predicted by RSM were10 M NaOH concentration, 1-hour CT and 0.5 g sample produced 32.45% lignin removal. While for coconut hull lignin removal were 10 M NaOH concentration, 12 hours CT, 0.5 g sample produced 59.47% removal of lignin. Results show that alkaline pre-treated rice hull and coconut hull able to be used to remove higher percentage of lignin.
本研究以农业废弃物中的稻壳和椰子壳为研究对象。测定了预处理前后稻壳和椰壳中木质素的含量。利用响应面法(RSM)预测最佳处理条件，利用傅里叶变换红外(FTIR)对木质素含量进行鉴定。考察了NaOH浓度、接触时间(CT)和样品质量的相互作用，以优化木质素去除率(%)。水稻壳木质素去除的二次模型的相关系数R2为0.8863，线性模型的椰子壳木质素去除的相关系数R2为0.7998,2FI模型的相关系数R2为0.8892。RSM预测稻壳木质素脱除的最佳条件为NaOH浓度为10 M, CT时间为1 h, 0.5 g样品木质素脱除率为32.45%。当NaOH浓度为10 M, CT时间为12 h时，0.5 g样品的木质素去除率为59.47%。结果表明，碱法预处理稻壳和椰壳对木质素的去除率较高。
{"title":"Alkaline pre-treatment of rice hull and coconut hull using Response Surface Methodology","authors":"Lee, R. Ying, Ashraf Muhammad, Rusli N. Dini, Mohammad Sohel Rahman, H. Harun, M. K..","doi":"10.47253/jtrss.v9i1.713","DOIUrl":"https://doi.org/10.47253/jtrss.v9i1.713","url":null,"abstract":"In this research, the rice hull and coconut hull from agriculture waste were investigated. The lignin content within the rice and coconut hull were determined before and after pre-treatment. Response Surface Methodology (RSM) used to predict the optimal condition for the treatments and Fourier Transform Infrared (FTIR) to identify the lignin content. The interaction of NaOH concentration, contact time (CT), and sample weight was investigated to optimize the lignin removal percentage (%). The correlation coefficient, R2 for a quadratic model of rice hull lignin removal was 0.8863 while for coconut hull lignin removal in the linear model was 0.7998, as well as the 2FI model was 0.8892. The optimum condition for rice hull lignin removal predicted by RSM were10 M NaOH concentration, 1-hour CT and 0.5 g sample produced 32.45% lignin removal. While for coconut hull lignin removal were 10 M NaOH concentration, 12 hours CT, 0.5 g sample produced 59.47% removal of lignin. Results show that alkaline pre-treated rice hull and coconut hull able to be used to remove higher percentage of lignin.","PeriodicalId":17457,"journal":{"name":"Journal of Tropical Resources and Sustainable Science (JTRSS)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73677139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pollen of Litchi chinensis (Litchi) is a major aeroallergen of Bangladesh. Pollen of this fruits plant was collected from full bloomed flower growing in different places of Rajshahi in Bangladesh. Pollen protein was extracted and partial purified by means of long-term PBS extraction, salting out, dialysis, gel filtrations and DEAE-Cellulose chromatography and the protein was designated as LFPP (Litchi flowers pollen protein). Gel filtration of the purified pollen protein gives two main peaks. The major peak gives four bands on SDS-PAGE. The enzyme (pectate lyase) proteins after gel filtration again re-purified by Ion exchange chromatography, a single band in the protein profile of LFPP, (M.W. 28kDa) was the major allergenic component of Litchi chinensis (Litchi) flower pollen. The homogeneity and the molecular weight of the protein were estimated by SDS-PAGE, and Gel filtration was 28kDa. The allergenic protein was identified by skin prick tests and showed the pectate lyase (Pel) activity. Skin-prick tests also revealed highest degree of sensitivity to the Nawabgang sample giving positive response in 80% of the patients. Skin reactivity ranged between 1+ and 3+.
{"title":"Characterization and Sensitivity Test of the Allergenic Pollen Proteins from Litchi Chimensis Plant","authors":"R. Shaha, N. Roy, G.Talukdar","doi":"10.47253/jtrss.v1i1.667","DOIUrl":"https://doi.org/10.47253/jtrss.v1i1.667","url":null,"abstract":"Pollen of Litchi chinensis (Litchi) is a major aeroallergen of Bangladesh. Pollen of this fruits plant was collected from full bloomed flower growing in different places of Rajshahi in Bangladesh. Pollen protein was extracted and partial purified by means of long-term PBS extraction, salting out, dialysis, gel filtrations and DEAE-Cellulose chromatography and the protein was designated as LFPP (Litchi flowers pollen protein). Gel filtration of the purified pollen protein gives two main peaks. The major peak gives four bands on SDS-PAGE. The enzyme (pectate lyase) proteins after gel filtration again re-purified by Ion exchange chromatography, a single band in the protein profile of LFPP, (M.W. 28kDa) was the major allergenic component of Litchi chinensis (Litchi) flower pollen. The homogeneity and the molecular weight of the protein were estimated by SDS-PAGE, and Gel filtration was 28kDa. The allergenic protein was identified by skin prick tests and showed the pectate lyase (Pel) activity. Skin-prick tests also revealed highest degree of sensitivity to the Nawabgang sample giving positive response in 80% of the patients. Skin reactivity ranged between 1+ and 3+.","PeriodicalId":17457,"journal":{"name":"Journal of Tropical Resources and Sustainable Science (JTRSS)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90352937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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