V. Belgaumkar, Varsha Baliram Bade, S. Pradhan, Gauri Bhale
Introduction: Methotrexate is an antifolate agent commonly used in various dermatological and rheumatological diseases such as psoriasis, systemic lupus erythematosus, and other connective tissue disorders. Acute toxicity manifesting as mucocutaneous ulcerations is a rare event in 3 - 10% of patients. Normal dosing commonly used for dermatologic and rheumatologic diseases is 15 - 25 mg/week. The main culprit leading to toxicity is the overdose of medication. Nausea, leukopenia, infections, gastrointestinal bleeding, renal impairment, etc. are the common manifestations of methotrexate toxicity. Mucocutaneous ulcerations, though infrequent, can appear as early as 3 - 7 days following methotrexate administration. Thus, it can be the imminent sign of methotrexate toxicity, providing a clue to its timely diagnosis. The crucial steps in the management of methotrexate toxicity are withdrawal of medication, immediate administration of leucovorin which is the biologically active form of folic acid, adequate hydration for increasing renal clearance, and urinary alkalinization with sodium bicarbonate, wherever necessary. Case Presentation: Here, we report an accidental methotrexate overdose in a patient with psoriasis, presenting with extensive mucocutaneous ulceration mimicking autoimmune vesiculobullous disorder and Stevens-Johnson syndrome- toxic epidermal necrolysis, leading to an extremely rare and challenging scenario. Conclusions: This case report emphasizes that careful history and evaluation of medical records facilitate early diagnosis and prompt management, which is critical to improving outcomes and patient’s survival.
{"title":"Mucocutaneous Ulcerations Due to Methotrexate Toxicity Mimicking Vesiculobullous Disorder: A Diagnostic Challenge","authors":"V. Belgaumkar, Varsha Baliram Bade, S. Pradhan, Gauri Bhale","doi":"10.5812/jssc-132386","DOIUrl":"https://doi.org/10.5812/jssc-132386","url":null,"abstract":"Introduction: Methotrexate is an antifolate agent commonly used in various dermatological and rheumatological diseases such as psoriasis, systemic lupus erythematosus, and other connective tissue disorders. Acute toxicity manifesting as mucocutaneous ulcerations is a rare event in 3 - 10% of patients. Normal dosing commonly used for dermatologic and rheumatologic diseases is 15 - 25 mg/week. The main culprit leading to toxicity is the overdose of medication. Nausea, leukopenia, infections, gastrointestinal bleeding, renal impairment, etc. are the common manifestations of methotrexate toxicity. Mucocutaneous ulcerations, though infrequent, can appear as early as 3 - 7 days following methotrexate administration. Thus, it can be the imminent sign of methotrexate toxicity, providing a clue to its timely diagnosis. The crucial steps in the management of methotrexate toxicity are withdrawal of medication, immediate administration of leucovorin which is the biologically active form of folic acid, adequate hydration for increasing renal clearance, and urinary alkalinization with sodium bicarbonate, wherever necessary. Case Presentation: Here, we report an accidental methotrexate overdose in a patient with psoriasis, presenting with extensive mucocutaneous ulceration mimicking autoimmune vesiculobullous disorder and Stevens-Johnson syndrome- toxic epidermal necrolysis, leading to an extremely rare and challenging scenario. Conclusions: This case report emphasizes that careful history and evaluation of medical records facilitate early diagnosis and prompt management, which is critical to improving outcomes and patient’s survival.","PeriodicalId":174870,"journal":{"name":"Journal of Skin and Stem Cell","volume":"7 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130828450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Pradhan, Mumyam Mitkong, V. Belgaumkar, A. Gosavi, N. Bhatt
Introduction: Bullous pemphigoid (BP) is the most common subepidermal autoimmune vesiculobullous disorder. It mainly affects the elderly, with a mean age at presentation of 69 to 83 years and an incidence of 7 to 43 per million population in European studies. Bullous pemphigoid (BP) is rarely encountered in young people, with an incidence rate of less than 0.5 cases per million population in individuals less than 50 years. Case Presentation: A 28-year-old male presented with fluid-filled lesions over his right forearm for three days. Also, an 18-year-old male presented with fluid-filled lesions over his face, hands, groin, and legs for two months. In both cases, histopathology and direct immunofluorescence confirmed BP. Conclusions: Though BP is rare in young individuals, it should be considered one of the differentials of vesiculobullous disorders. Herein, we reported two cases of BP in young people at unusual sites.
{"title":"Unusual Presentations of Bullous Pemphigoid in Young Patients: A Case Series","authors":"S. Pradhan, Mumyam Mitkong, V. Belgaumkar, A. Gosavi, N. Bhatt","doi":"10.5812/jssc-131978","DOIUrl":"https://doi.org/10.5812/jssc-131978","url":null,"abstract":"Introduction: Bullous pemphigoid (BP) is the most common subepidermal autoimmune vesiculobullous disorder. It mainly affects the elderly, with a mean age at presentation of 69 to 83 years and an incidence of 7 to 43 per million population in European studies. Bullous pemphigoid (BP) is rarely encountered in young people, with an incidence rate of less than 0.5 cases per million population in individuals less than 50 years. Case Presentation: A 28-year-old male presented with fluid-filled lesions over his right forearm for three days. Also, an 18-year-old male presented with fluid-filled lesions over his face, hands, groin, and legs for two months. In both cases, histopathology and direct immunofluorescence confirmed BP. Conclusions: Though BP is rare in young individuals, it should be considered one of the differentials of vesiculobullous disorders. Herein, we reported two cases of BP in young people at unusual sites.","PeriodicalId":174870,"journal":{"name":"Journal of Skin and Stem Cell","volume":"7 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125750569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Khalili, S. Shamsi meymandi, Rezvan Amiri, Mahin Aflatoonian, Fatemeh Ghayoori, Niloofar Mehrolhasani
Background: Bullous diseases are classified as autoimmune blistering diseases, hereditary blistering disorders, and blistering diseases secondary to inflammation and physical trauma. This study evaluated clinicopathological features of patients with bullous diseases. Methods: This is a retrospective cross-sectional study of 88 patients with vesiculobullous diseases referred to the dermatology clinic of Afzalipour hospital in Kerman, Iran. Demographic features of the patients, type of lesions, differential diagnosis, and pathological features (site of skin biopsy, final pathological diagnosis, type of inflammatory cells, and direct immunofluorescence results) were recorded. Data were analyzed by chi-square test and independent t-test. Results: Eighty-eight patients (59.1% females and 40.9% males) were evaluated. The mean age of patients was 45.09 ± 20.48 years. Autoimmune blistering diseases, hereditary bullous diseases, and blisters secondary to inflammation and trauma were observed in 79.5%, 11.4%, 6.8%, and 2.3% of the cases, respectively. The most common diseases were pemphigus vulgaris (29.5%) and bullous pemphigoid (21.6%). There was a significant correlation between the type of the disease and the age of patients (P-value = 0.001) and the duration of the disease (0.047). Conclusions: The most common autoimmune blistering diseases, hereditary bullous diseases, and blisters secondary to inflammation and trauma were pemphigus vulgaris, epidermolysis bullosa, lichen planus, and diabetic bullae/friction blister, respectively.
{"title":"Clinicopathological Features of Patients with Vesiculobullous Diseases Referred to Dermatology Clinic of Afzalipour Hospital, Kerman, Iran","authors":"M. Khalili, S. Shamsi meymandi, Rezvan Amiri, Mahin Aflatoonian, Fatemeh Ghayoori, Niloofar Mehrolhasani","doi":"10.5812/jssc-129556","DOIUrl":"https://doi.org/10.5812/jssc-129556","url":null,"abstract":"Background: Bullous diseases are classified as autoimmune blistering diseases, hereditary blistering disorders, and blistering diseases secondary to inflammation and physical trauma. This study evaluated clinicopathological features of patients with bullous diseases. Methods: This is a retrospective cross-sectional study of 88 patients with vesiculobullous diseases referred to the dermatology clinic of Afzalipour hospital in Kerman, Iran. Demographic features of the patients, type of lesions, differential diagnosis, and pathological features (site of skin biopsy, final pathological diagnosis, type of inflammatory cells, and direct immunofluorescence results) were recorded. Data were analyzed by chi-square test and independent t-test. Results: Eighty-eight patients (59.1% females and 40.9% males) were evaluated. The mean age of patients was 45.09 ± 20.48 years. Autoimmune blistering diseases, hereditary bullous diseases, and blisters secondary to inflammation and trauma were observed in 79.5%, 11.4%, 6.8%, and 2.3% of the cases, respectively. The most common diseases were pemphigus vulgaris (29.5%) and bullous pemphigoid (21.6%). There was a significant correlation between the type of the disease and the age of patients (P-value = 0.001) and the duration of the disease (0.047). Conclusions: The most common autoimmune blistering diseases, hereditary bullous diseases, and blisters secondary to inflammation and trauma were pemphigus vulgaris, epidermolysis bullosa, lichen planus, and diabetic bullae/friction blister, respectively.","PeriodicalId":174870,"journal":{"name":"Journal of Skin and Stem Cell","volume":"40 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124784117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Therapeutic Pearl: Cyanoacrylate Glue is a Good Thermo-dehydro-anti-infective Sealing Agent for Treating Digital Chronic Intertrigo","authors":"M. Mukhtar, N. Mukhtar","doi":"10.5812/jssc-129914","DOIUrl":"https://doi.org/10.5812/jssc-129914","url":null,"abstract":"<jats:p />","PeriodicalId":174870,"journal":{"name":"Journal of Skin and Stem Cell","volume":"14 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121235566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Innovative Use of a Disposable Syringe Barrel as a Device to Drain a Cyst","authors":"M. Mukhtar, Sanjeeva Gupta","doi":"10.5812/jssc-129915","DOIUrl":"https://doi.org/10.5812/jssc-129915","url":null,"abstract":"<jats:p />","PeriodicalId":174870,"journal":{"name":"Journal of Skin and Stem Cell","volume":"48 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115168414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Pradhan, Mariya Kharodawala, V. Belgaumkar, A. Gosavi, N. Bhatt
Introduction: Palmoplantar keratodermas (PPK) constitute a clinically and genetically complex group of cornification disorders characterized by defective epidermal differentiation leading to marked palmoplantar hyperkeratosis. Classically, keratodermas have been categorised as hereditary and acquired forms. Case Presentation: A 12-year-old male patient presented to our dermatology outpatient department with diffuse thickening and yellowish discoloration of skin over palms and soles since early childhood. He gradually developed progressive thickening and contractures of the fingers of both hands with functional impairment. No such history could be elicited in the family members. Dermatological examination showed bilaterally symmetrical thick hyperkeratotic fissured scaly plaques involving surface of entire palm with sharp demarcation at volar aspect of wrist. Similar plaques with yellowish hue were present over the soles with superficial fissures. The histopathological examination of skin biopsy specimen from the palm showed focal parakeratosis alternating with orthokeratosis in both horizontal and vertical directions (checkerboard pattern) with broadened rete ridges. The findings were consistent with pityriasis rubra pilaris (PRP). The patient improved with systemic and topical retinoids. Conclusions: This report focuses on the recognition of unusual presentations of PRP and emphasizes the importance of early diagnosis and timely treatment, which can avert the dreaded morbid and disabling complication of contractures.
{"title":"Debilitating Contractures of Hands in an Atypical Presentation of Juvenile Pityriasis Rubra Pilaris: A Rare Case Report","authors":"S. Pradhan, Mariya Kharodawala, V. Belgaumkar, A. Gosavi, N. Bhatt","doi":"10.5812/jssc-130311","DOIUrl":"https://doi.org/10.5812/jssc-130311","url":null,"abstract":"Introduction: Palmoplantar keratodermas (PPK) constitute a clinically and genetically complex group of cornification disorders characterized by defective epidermal differentiation leading to marked palmoplantar hyperkeratosis. Classically, keratodermas have been categorised as hereditary and acquired forms. Case Presentation: A 12-year-old male patient presented to our dermatology outpatient department with diffuse thickening and yellowish discoloration of skin over palms and soles since early childhood. He gradually developed progressive thickening and contractures of the fingers of both hands with functional impairment. No such history could be elicited in the family members. Dermatological examination showed bilaterally symmetrical thick hyperkeratotic fissured scaly plaques involving surface of entire palm with sharp demarcation at volar aspect of wrist. Similar plaques with yellowish hue were present over the soles with superficial fissures. The histopathological examination of skin biopsy specimen from the palm showed focal parakeratosis alternating with orthokeratosis in both horizontal and vertical directions (checkerboard pattern) with broadened rete ridges. The findings were consistent with pityriasis rubra pilaris (PRP). The patient improved with systemic and topical retinoids. Conclusions: This report focuses on the recognition of unusual presentations of PRP and emphasizes the importance of early diagnosis and timely treatment, which can avert the dreaded morbid and disabling complication of contractures.","PeriodicalId":174870,"journal":{"name":"Journal of Skin and Stem Cell","volume":"101 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129876840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Poutoglidis, P. Karamitsou, Antonios Skalias, N. Tsetsos, E. Forozidou
Introduction: A 76-year-old male presented to our department with a neoplastic growth on his left auricle. Case Presentation: The patient was referred from another hospital because he was very concerned about the cosmetic outcome. He had been refused an extensive plastic intervention due to his age and comorbidities. Clinical examination revealed a 1.3 × 1.4 cm ulcerative growth on the upper left ear helix. After the total excision of the lesion, malignant squamous cell carcinoma was diagnosed under frozen sections. A helical rim advancement flap was recruited to achieve the best possible reconstructive outcome. Conclusions: Surgery for auricle malignancies may be performed in one stage without prior biopsy if frozen sections are positive in highly suspicious cases. Aesthetic outcomes should be considered for every patient, regardless of gender or age.
{"title":"Helical Rim Advancement Flap and Its Implication in the Skin Cancer of the Auricle: A Case Report","authors":"A. Poutoglidis, P. Karamitsou, Antonios Skalias, N. Tsetsos, E. Forozidou","doi":"10.5812/jssc-130108","DOIUrl":"https://doi.org/10.5812/jssc-130108","url":null,"abstract":"Introduction: A 76-year-old male presented to our department with a neoplastic growth on his left auricle. Case Presentation: The patient was referred from another hospital because he was very concerned about the cosmetic outcome. He had been refused an extensive plastic intervention due to his age and comorbidities. Clinical examination revealed a 1.3 × 1.4 cm ulcerative growth on the upper left ear helix. After the total excision of the lesion, malignant squamous cell carcinoma was diagnosed under frozen sections. A helical rim advancement flap was recruited to achieve the best possible reconstructive outcome. Conclusions: Surgery for auricle malignancies may be performed in one stage without prior biopsy if frozen sections are positive in highly suspicious cases. Aesthetic outcomes should be considered for every patient, regardless of gender or age.","PeriodicalId":174870,"journal":{"name":"Journal of Skin and Stem Cell","volume":"16 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130204862","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Scalp Erythema and Nodules: Would You Think of Primary Cutaneous Follicle Center B-Cell Lymphoma if a Biopsy is Inconclusive?","authors":"F. Etaee, S. Wright, J. Yeary","doi":"10.5812/jssc-130174","DOIUrl":"https://doi.org/10.5812/jssc-130174","url":null,"abstract":"<jats:p />","PeriodicalId":174870,"journal":{"name":"Journal of Skin and Stem Cell","volume":"325 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124586470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Samaneh Noroozi, F. Fadaei, M. Rahbar, M. Tabarrai, P. Mansouri, L. Shirbeigi
: Freckles are common pigmented lesions frequently observed on the face and other sun-exposed areas of the skin. Ephelides have an autosomal dominant inheritance, indicating that melanocyte activity and sun exposure are significant risk factors for the development of these lesions. The importance of freckles can be discussed from two perspectives of cosmetics and their complications. This research is the first step in gaining access to extensive scientific and experimental resources of Persian medicine (PM). "Barash" and "Namash" are the equivalent terms for freckles in medieval manuscripts of Persia. Therefore, words related to "Barash & Namash" from PM ancient manuscripts and other relevant keywords were searched in Google Scholar, PubMed, and Scopus databases. Extracted results were subsequently compared and recorded under the following classification. The clinical symptoms of freckles were similar to those of "Namash & Barash" diseases. Different therapeutic methods to treat such diseases in PM include lifestyle modification, medication, and manual intervention. There are several preventive and therapeutic methods for the management of freckles. Further research is required in this regard to identify more academic evidence.
{"title":"Novel Preventive and Therapeutic Strategies for Ephelides (Freckles) from a Persian Medicine Perspective: A Narrative Review","authors":"Samaneh Noroozi, F. Fadaei, M. Rahbar, M. Tabarrai, P. Mansouri, L. Shirbeigi","doi":"10.5812/jssc-123335","DOIUrl":"https://doi.org/10.5812/jssc-123335","url":null,"abstract":": Freckles are common pigmented lesions frequently observed on the face and other sun-exposed areas of the skin. Ephelides have an autosomal dominant inheritance, indicating that melanocyte activity and sun exposure are significant risk factors for the development of these lesions. The importance of freckles can be discussed from two perspectives of cosmetics and their complications. This research is the first step in gaining access to extensive scientific and experimental resources of Persian medicine (PM). \"Barash\" and \"Namash\" are the equivalent terms for freckles in medieval manuscripts of Persia. Therefore, words related to \"Barash & Namash\" from PM ancient manuscripts and other relevant keywords were searched in Google Scholar, PubMed, and Scopus databases. Extracted results were subsequently compared and recorded under the following classification. The clinical symptoms of freckles were similar to those of \"Namash & Barash\" diseases. Different therapeutic methods to treat such diseases in PM include lifestyle modification, medication, and manual intervention. There are several preventive and therapeutic methods for the management of freckles. Further research is required in this regard to identify more academic evidence.","PeriodicalId":174870,"journal":{"name":"Journal of Skin and Stem Cell","volume":"89 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133386273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: Elastin peptides stimulate the development of mouse melanocytes in neural crest culture. Ferrous ferric chloride (FFC) promotes mammalian melanocyte growth in culture. However, it is unclear whether elastin peptides in the presence of FFC can stimulate human melanocyte growth in situ. Objectives: This study aimed to investigate the mechanism of human melanocyte growth for skin and stem cell science since melanocytes control human skin color. Methods: In this clinical trial study, a lotion containing elastin peptides and/or FFC was applied to the normal skin of 6 volunteers twice a day for 1 to 3 months. Punch biopsies were taken from treated skin and surveyed by histochemical methods using the dopa reaction (detect melanocytes) and dopa-premelanin reaction (detect melanocytes and melanoblasts). Elastin fibers were detected by Victoria blue staining. Results: Only the combined treatment of elastin peptides and FFC increased melanocyte populations in addition to dopa reactivity, melanogenesis, dendritogenesis, and epidermal melanin pigmentation. Mitotic divisions of melanocytes were also observed. However, the melanoblast population failed to increase, and no mitotic melanoblasts were observed. In the dermis, elastin fibers became thicker and denser after the treatment. The data of statistical analyses were performed by tabulation, mean, and SD on Microsoft Excel for Macintosh OS Catalina 10 system. Conclusions: Our present study suggests that elastin peptides with FFC can promote melanocyte growth, melanin synthesis, skin pigmentation, and elastin fiber formation. Our study can be expected to contribute to advancing skin and stem cell science.
背景:弹性蛋白肽刺激小鼠神经嵴培养中黑素细胞的发育。氯化亚铁(FFC)促进哺乳动物黑素细胞的生长。然而，目前尚不清楚FFC存在下的弹性蛋白肽是否能刺激人黑素细胞原位生长。目的:探讨黑素细胞控制人皮肤颜色的生长机制，为皮肤和干细胞科学提供科学依据。方法:在这项临床试验研究中，将含有弹性蛋白肽和/或FFC的洗液涂抹在6名志愿者的正常皮肤上，每天两次，持续1至3个月。从处理过的皮肤上取活检组织，用组织化学方法检测多巴反应(检测黑素细胞)和多巴-黑色素前反应(检测黑素细胞和成黑素细胞)。维多利亚蓝染色检测弹性蛋白纤维。结果:只有弹性蛋白肽和FFC联合处理，除了多巴反应性、黑色素生成、树突生成和表皮黑色素沉着外，黑素细胞数量也增加。同时观察到黑素细胞有丝分裂。然而，黑素母细胞数量没有增加，没有观察到有丝分裂的黑素母细胞。在真皮中，弹性蛋白纤维在治疗后变得更粗、更密。统计分析数据在Microsoft Excel for Macintosh OS Catalina 10操作系统上采用制表法、均数法和标准差法进行。结论:我们目前的研究表明，含有FFC的弹性蛋白肽可以促进黑素细胞生长、黑色素合成、皮肤色素沉着和弹性蛋白纤维的形成。我们的研究有望促进皮肤和干细胞科学的发展。
{"title":"Elastin Peptides with Ferrous Ferric Chloride Activate Human Melanocytes and Elastin Fibers","authors":"T. Hirobe, H. Enami","doi":"10.5812/jssc-127254","DOIUrl":"https://doi.org/10.5812/jssc-127254","url":null,"abstract":"Background: Elastin peptides stimulate the development of mouse melanocytes in neural crest culture. Ferrous ferric chloride (FFC) promotes mammalian melanocyte growth in culture. However, it is unclear whether elastin peptides in the presence of FFC can stimulate human melanocyte growth in situ. Objectives: This study aimed to investigate the mechanism of human melanocyte growth for skin and stem cell science since melanocytes control human skin color. Methods: In this clinical trial study, a lotion containing elastin peptides and/or FFC was applied to the normal skin of 6 volunteers twice a day for 1 to 3 months. Punch biopsies were taken from treated skin and surveyed by histochemical methods using the dopa reaction (detect melanocytes) and dopa-premelanin reaction (detect melanocytes and melanoblasts). Elastin fibers were detected by Victoria blue staining. Results: Only the combined treatment of elastin peptides and FFC increased melanocyte populations in addition to dopa reactivity, melanogenesis, dendritogenesis, and epidermal melanin pigmentation. Mitotic divisions of melanocytes were also observed. However, the melanoblast population failed to increase, and no mitotic melanoblasts were observed. In the dermis, elastin fibers became thicker and denser after the treatment. The data of statistical analyses were performed by tabulation, mean, and SD on Microsoft Excel for Macintosh OS Catalina 10 system. Conclusions: Our present study suggests that elastin peptides with FFC can promote melanocyte growth, melanin synthesis, skin pigmentation, and elastin fiber formation. Our study can be expected to contribute to advancing skin and stem cell science.","PeriodicalId":174870,"journal":{"name":"Journal of Skin and Stem Cell","volume":"48 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126389907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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