首页 > 最新文献

Journal of Tropical Medicine最新文献

英文 中文
Larvicidal, Ovicidal, and Repellent Activities of Leucas stachydiformis (Hochst. ex Benth.) Briq Essential Oil against Anopheles arabiensis Leucas stachydiformis (Hochst. ex Benth.) Briq 精油对阿拉伯按蚊的杀幼虫、杀卵和驱虫活性
IF 2.2 4区 医学 Q3 PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH Pub Date : 2024-03-29 DOI: 10.1155/2024/1051086
Sisay Fikru, Ketema Tolossa, Peter Lindemann, Franz Bucar, Kaleab Asres
Larvicidal, ovicidal, and repellent activities of the essential oil extracted by hydrodistillation from the leaves of the endemic Ethiopian plant Leucas stachydiformis (Hochst. ex Benth.) Briq were investigated against Anopheles arabiensis, the dominant malaria vector species in Ethiopia with the objective of searching for a plant-based malaria vector control strategy from medicinal plants. The larvicidal effect was tested against the fourth instar An. arabiensis wild larvae whilst freshly laid ova of An. arabiensis were used to determine the ovicidal activity of the essential oil at concentrations ranging from 6.25 to 400 ppm. Concentrations of 41.6–366.7 µg/cm2 were used to evaluate the repellent activity of the essential oil on 3–5 days old adult female An. arabiensis. The oil composition of L. stachydiformis was also analyzed using GC-MS. The study revealed that the oil possesses the highest larvicidal activity at 400 ppm and 200 ppm after 24 h and 48 h of treatment. LC50 values for the fourth larval instar after 24 h and 48 h of treatment were 43.4 ppm and 34.2 ppm, respectively. After 72 h of exposure, the oil displayed 100% ovicidal activity at 400 ppm with an IH50 value of 32.2 ppm. In the repellency test, at concentrations of 366.7, 133.3, and 41.6 µg/cm2, the oil gave a total percentage protection of 67.9 ± 4.2%, 37.2 ± 2.8%, and 32 ± 2.2%, respectively, for 4 h. The highest concentration (366.7 µg/cm2) gave 100% protection up to 90 min. GC-MS analysis of the oil revealed the presence of 24 compounds representing 90.34% of the total oil with caryophyllene oxide, germacrene D, and trans-caryophyllene constituting more than 50% of its components. Results of the present study suggest that the essential oil of L. stachydiformis has the potential to be used for the control of An. arabiensis mosquitoes.
研究了从埃塞俄比亚特有植物 Leucas stachydiformis (Hochst. ex Benth.) Briq 的叶子中通过水蒸馏提取的精油对埃塞俄比亚主要疟疾病媒物种阿拉伯按蚊(Anopheles arabiensis)的杀幼虫、杀卵和驱虫活性,目的是从药用植物中寻找一种基于植物的疟疾病媒控制策略。对阿拉伯疟蚊第四龄野生幼虫的杀幼虫效果进行了测试,同时用刚产卵的阿拉伯疟蚊幼虫来确定精油在 6.25 至 400 ppm 浓度范围内的杀卵活性。用 41.6-366.7 µg/cm2 的浓度来评估精油对 3-5 天大的成年雌性阿拉伯蚂蚁的驱避活性。此外,还使用气相色谱-质谱分析了 L. stachydiformis 的精油成分。研究表明,在处理 24 小时和 48 小时后,精油在 400 ppm 和 200 ppm 的浓度下具有最高的杀幼虫活性。处理 24 小时和 48 小时后,第四龄幼虫的 LC50 值分别为 43.4 ppm 和 34.2 ppm。接触 72 小时后,在浓度为百万分之 400 时,该油显示出 100% 的杀卵活性,IH50 值为百万分之 32.2。在驱避试验中,浓度分别为 366.7、133.3 和 41.6 µg/cm2 的精油在 4 小时内的总保护率分别为 67.9 ± 4.2%、37.2 ± 2.8% 和 32 ± 2.2%。 最高浓度(366.7 µg/cm2)可在 90 分钟内提供 100% 的保护。对油的气相色谱-质谱分析显示,油中含有 24 种化合物,占油总量的 90.34%,其中氧化叶黄素、胚芽烯 D 和反式叶黄素占其成分的 50%以上。本研究的结果表明,L. stachydiformis 的精油具有用于控制阿拉伯蚊子的潜力。
{"title":"Larvicidal, Ovicidal, and Repellent Activities of Leucas stachydiformis (Hochst. ex Benth.) Briq Essential Oil against Anopheles arabiensis","authors":"Sisay Fikru, Ketema Tolossa, Peter Lindemann, Franz Bucar, Kaleab Asres","doi":"10.1155/2024/1051086","DOIUrl":"https://doi.org/10.1155/2024/1051086","url":null,"abstract":"Larvicidal, ovicidal, and repellent activities of the essential oil extracted by hydrodistillation from the leaves of the endemic Ethiopian plant <i>Leucas stachydiformis</i> (Hochst. ex Benth.) Briq were investigated against <i>Anopheles arabiensis</i>, the dominant malaria vector species in Ethiopia with the objective of searching for a plant-based malaria vector control strategy from medicinal plants. The larvicidal effect was tested against the fourth instar <i>An. arabiensis</i> wild larvae whilst freshly laid ova of <i>An</i>. <i>arabiensis</i> were used to determine the ovicidal activity of the essential oil at concentrations ranging from 6.25 to 400 ppm. Concentrations of 41.6–366.7 <i>µ</i>g/cm<sup>2</sup> were used to evaluate the repellent activity of the essential oil on 3–5 days old adult female <i>An. arabiensis</i>. The oil composition of <i>L. stachydiformis</i> was also analyzed using GC-MS. The study revealed that the oil possesses the highest larvicidal activity at 400 ppm and 200 ppm after 24 h and 48 h of treatment. LC<sub>50</sub> values for the fourth larval instar after 24 h and 48 h of treatment were 43.4 ppm and 34.2 ppm, respectively. After 72 h of exposure, the oil displayed 100% ovicidal activity at 400 ppm with an IH<sub>50</sub> value of 32.2 ppm. In the repellency test, at concentrations of 366.7, 133.3, and 41.6 µg/cm<sup>2</sup>, the oil gave a total percentage protection of 67.9 ± 4.2%, 37.2 ± 2.8%, and 32 ± 2.2%, respectively, for 4 h. The highest concentration (366.7 <i>µ</i>g/cm<sup>2</sup>) gave 100% protection up to 90 min. GC-MS analysis of the oil revealed the presence of 24 compounds representing 90.34% of the total oil with caryophyllene oxide, germacrene D, and <i>trans</i>-caryophyllene constituting more than 50% of its components. Results of the present study suggest that the essential oil of <i>L</i>. <i>stachydiformis</i> has the potential to be used for the control of <i>An. arabiensis</i> mosquitoes.","PeriodicalId":17527,"journal":{"name":"Journal of Tropical Medicine","volume":"2 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140323341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Incidence of Helminthic and Viral Coinfections in Malaria Patients in the Tertiary Care Hospital Setup 三级医院疟疾患者螺旋体和病毒合并感染的发病率
IF 2.2 4区 医学 Q3 PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH Pub Date : 2024-03-28 DOI: 10.1155/2024/8529788
Murad A. Mubaraki, Mubbashir Hussain, Fozia Fozia, Ijaz Ahmad, Shahid Niaz Khan, Abdul Qadir Khan, Ziaullah Ziaullah
Introduction. This study determines the incidence of common viral and helminth coinfections with malaria in the tertiary care hospital set up in southern Khyber Pakhtunkhwa, Pakistan. Materials and Methods. The multidimensional research included malaria patients admitted to different hospitals of district Kohat during January and December 2021. Stool samples and blood were assembled from the patients. Giemsa-stained microscopy-positive samples were processed by the immunochromatography technique (ICT) to identify Plasmodium species. Common viral infections such as viral hepatitis (A, B, and C), HIV, and dengue (DENV) were analyzed by ICT kits while SARS-CoV-2 was confirmed through real-time PCR. Furthermore, the intestinal helminths were identified using the Kato-Katz thick smear method. Results. Among 1278 patients, 548 were diagnosed with malaria, 412 (75.2%) were positive for P. vivax infection, 115 (21%) for P. falciparum, and 21 (3.8%) for mixed malaria infection (P. vivax/P. falciparum), with a higher incidence among males (65.2%) than females (34.8%). Coinfection with helminths was positive in 215 (39.3%) malaria patients. The most common infections were caused by the Ascaris lumbricoides species (42.6%) followed by Enterobius vermicularis (31.7%) and hookworm. A total of 24.6% of malaria-positive cases were also coinfected with different viruses with higher frequencies of confection for HAV (8.2%) and DENV (6.2%), respectively. The patients revealed higher incidence of coinfections with P. falciparum (57%) as compared with P. vivax (39.2%) and mixed infections (3.7%). Conclusion. This study demonstrated that the study population exhibited a significant incidence of coinfections with intestinal helminth and viral malaria.
导言。本研究确定了巴基斯坦开伯尔-普赫图赫瓦省南部三级医院中常见病毒和螺旋体与疟疾并发感染的发病率。材料和方法。这项多维研究包括 2021 年 1 月至 12 月期间科哈特地区不同医院收治的疟疾患者。采集了患者的粪便样本和血液样本。用免疫层析技术(ICT)处理革兰氏染色显微镜检查呈阳性的样本,以确定疟原虫的种类。常见的病毒感染,如病毒性肝炎(甲型、乙型和丙型)、艾滋病病毒和登革热(DENV),则通过 ICT 试剂盒进行分析,而 SARS-CoV-2 则通过实时 PCR 进行确认。此外,还使用 Kato-Katz 厚涂片法对肠道蠕虫进行了鉴定。结果在 1278 名患者中,548 人被确诊为疟疾患者,其中 412 人(75.2%)对间日疟原虫感染呈阳性反应,115 人(21%)对恶性疟原虫感染呈阳性反应,21 人(3.8%)对混合疟疾感染(间日疟原虫/恶性疟原虫)呈阳性反应,男性发病率(65.2%)高于女性(34.8%)。215名疟疾患者(39.3%)的螺旋体合并感染呈阳性。最常见的感染由蛔虫(42.6%)引起,其次是蠕虫(31.7%)和钩虫。共有 24.6% 的疟疾阳性病例同时感染了不同的病毒,其中感染 HAV(8.2%)和 DENV(6.2%)的频率较高。与恶性疟原虫(39.2%)和混合感染(3.7%)相比,患者合并感染恶性疟原虫的比例更高(57%)。结论这项研究表明,研究人群中合并感染肠道蠕虫疟疾和病毒性疟疾的发病率很高。
{"title":"Incidence of Helminthic and Viral Coinfections in Malaria Patients in the Tertiary Care Hospital Setup","authors":"Murad A. Mubaraki, Mubbashir Hussain, Fozia Fozia, Ijaz Ahmad, Shahid Niaz Khan, Abdul Qadir Khan, Ziaullah Ziaullah","doi":"10.1155/2024/8529788","DOIUrl":"https://doi.org/10.1155/2024/8529788","url":null,"abstract":"<i>Introduction</i>. This study determines the incidence of common viral and helminth coinfections with malaria in the tertiary care hospital set up in southern Khyber Pakhtunkhwa, Pakistan. <i>Materials and Methods</i>. The multidimensional research included malaria patients admitted to different hospitals of district Kohat during January and December 2021. Stool samples and blood were assembled from the patients. Giemsa-stained microscopy-positive samples were processed by the immunochromatography technique (ICT) to identify <i>Plasmodium</i> species. Common viral infections such as viral hepatitis (A, B, and C), HIV, and dengue (DENV) were analyzed by ICT kits while SARS-CoV-2 was confirmed through real-time PCR. Furthermore, the intestinal helminths were identified using the Kato-Katz thick smear method. <i>Results</i>. Among 1278 patients, 548 were diagnosed with malaria, 412 (75.2%) were positive for <i>P. vivax</i> infection, 115 (21%) for <i>P. falciparum</i>, and 21 (3.8%) for mixed malaria infection (<i>P. vivax/P. falciparum</i>), with a higher incidence among males (65.2%) than females (34.8%). Coinfection with helminths was positive in 215 (39.3%) malaria patients. The most common infections were caused by the <i>Ascaris lumbricoides</i> species (42.6%) followed by <i>Enterobius vermicularis</i> (31.7%) and hookworm. A total of 24.6% of malaria-positive cases were also coinfected with different viruses with higher frequencies of confection for HAV (8.2%) and DENV (6.2%), respectively. The patients revealed higher incidence of coinfections with <i>P. falciparum</i> (57%) as compared with <i>P. vivax</i> (39.2%) and mixed infections (3.7%). <i>Conclusion</i>. This study demonstrated that the study population exhibited a significant incidence of coinfections with intestinal helminth and viral malaria.","PeriodicalId":17527,"journal":{"name":"Journal of Tropical Medicine","volume":"9 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140316966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of Malaria and Schistosoma mansoni Coinfection on Selected Biochemical Profiles among Patients Attending Selected Health Institutions at Dembiya, Northwest Ethiopia 疟疾和曼氏血吸虫合并感染对埃塞俄比亚西北部登比亚部分医疗机构就诊患者部分生化指标的影响
IF 2.2 4区 医学 Q3 PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH Pub Date : 2024-03-21 DOI: 10.1155/2024/9992233
Wagaw Abebe, Wossenseged Lemma, Yalewayker Tegegne, Amare Mekuanint, Abebe Yenesew, Adane Derso
<i>Background</i>. Malaria and schistosomiasis are infectious diseases that cause biochemical abnormalities. Malaria and <i>Schistosoma mansoni</i> coinfection causes exacerbations of health consequences and comorbidities. The study area is found in Ethiopia, where coinfection of malaria and <i>S. mansoni</i> is common. However, there is limited data on the biochemical profiles of patients coinfected with malaria and <i>S. mansoni</i> schistosomiasis in the study area. Hence, this study aimed to assess the effect of malaria and <i>S. mansoni</i> schistosomiasis coinfection on selected biochemical profiles. <i>Methods</i>. An institutional-based comparative cross-sectional study was conducted from March 30 to August 10, 2022. Using a convenient sampling technique, 70 participants (35 cases and 35 controls) were enrolled in the study. <i>Schistosoma mansoni</i> was detected in stool samples using the wet mount and the Kato Katz method. To detect <i>Plasmodium</i>, both thick and thin blood films were prepared and stained with Giemsa. The blood sample was processed for the analysis of biochemical profiles. All data were analyzed using SPSS version 25. A <svg height="10.2124pt" style="vertical-align:-3.42943pt" version="1.1" viewbox="-0.0498162 -6.78297 7.83752 10.2124" width="7.83752pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"></path></g></svg> value of less than 0.05 was considered statistically significant. <i>Results</i>. The mean values of alanine aminotransferase and aspartate aminotransferase (37.1 U/L and 41.9 U/L, respectively) in coinfected participants were significantly higher than in the healthy control participants (17.4 U/L and 22.0 U/L, respectively) <span><svg height="12.7178pt" style="vertical-align:-3.42947pt" version="1.1" viewbox="-0.0498162 -9.28833 23.471 12.7178" width="23.471pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"></path></g><g transform="matrix(.013,0,0,-0.013,4.498,0)"><use xlink:href="#g113-113"></use></g><g transform="matrix(.013,0,0,-0.013,15.84,0)"></path></g></svg><span></span><span><svg height="12.7178pt" style="vertical-align:-3.42947pt" version="1.1" viewbox="27.053183800000003 -9.28833 26.453 12.7178" width="26.453pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,27.103,0)"></path></g><g transform="matrix(.013,0,0,-0.013,33.343,0)"></path></g><g transform="matrix(.013,0,0,-0.013,36.307,0)"><use xlink:href="#g113-49"></use></g><g transform="matrix(.013,0,0,-0.013,42.547,0)"></path></g><g transform="matrix(.013,0,0,-0.013,48.787,0)"></path></g></svg>.</span></span> Also, the median values of creatinine, total bilirubin, and direct bilirubin (1.51 mg/dL, 2.35 mg/dL, and 0.91 mg/dL, respectively) in coinfected participants were significantly higher than in the healthy control participants
背景。疟疾和血吸虫病是导致生化异常的传染病。疟疾和曼氏血吸虫合并感染会加重健康后果和合并症。研究地区位于埃塞俄比亚,那里疟疾和曼氏血吸虫合并感染很常见。然而,关于该研究地区疟疾和曼森血吸虫病合并感染患者的生化指标的数据却很有限。因此,本研究旨在评估疟疾和曼氏血吸虫病合并感染对某些生化指标的影响。研究方法于 2022 年 3 月 30 日至 8 月 10 日开展了一项以机构为基础的横断面比较研究。研究采用方便抽样技术,共招募了 70 名参与者(35 例病例和 35 例对照)。采用湿装载法和加藤卡茨法检测粪便样本中的曼氏血吸虫。为检测疟原虫,制备了厚和薄的血片,并用吉氏染色法染色。血样经处理后用于分析生化指标。所有数据均使用 SPSS 25 版进行分析。小于 0.05 的数值被认为具有统计学意义。结果合并感染者的丙氨酸氨基转移酶和天冬氨酸氨基转移酶平均值(分别为 37.1 U/L和 41.9 U/L)明显高于健康对照者(分别为 17.4 U/L和 22.0 U/L)。此外,合并感染者的肌酐、总胆红素和直接胆红素的中位值(分别为 1.51 毫克/分升、2.35 毫克/分升和 0.91 毫克/分升)也明显高于健康对照者(分别为 0.85 毫克/分升、0.42 毫克/分升和 0.12 毫克/分升)。然而,合并感染者的总蛋白中位值(4.82 克/分升)和葡萄糖平均值(66.6 毫克/分升)明显低于健康对照者(总蛋白(7.64 克/分升)和葡萄糖(91.9 毫克/分升))。在疟疾和曼氏血吸虫合并感染中,健康参与者的生化指标结果与曼氏血吸虫轻度、中度和重度感染者有明显差异。除总蛋白和葡萄糖与生化指标呈负相关外,曼氏血吸虫感染强度与生化指标呈正相关。结论与健康人相比,合并感染者的生化指标发生了显著变化。因此,应利用生化指标检测来监测和管理与合并感染相关的问题,并降低与合并感染相关的发病率和死亡率。
{"title":"Effect of Malaria and Schistosoma mansoni Coinfection on Selected Biochemical Profiles among Patients Attending Selected Health Institutions at Dembiya, Northwest Ethiopia","authors":"Wagaw Abebe, Wossenseged Lemma, Yalewayker Tegegne, Amare Mekuanint, Abebe Yenesew, Adane Derso","doi":"10.1155/2024/9992233","DOIUrl":"https://doi.org/10.1155/2024/9992233","url":null,"abstract":"&lt;i&gt;Background&lt;/i&gt;. Malaria and schistosomiasis are infectious diseases that cause biochemical abnormalities. Malaria and &lt;i&gt;Schistosoma mansoni&lt;/i&gt; coinfection causes exacerbations of health consequences and comorbidities. The study area is found in Ethiopia, where coinfection of malaria and &lt;i&gt;S. mansoni&lt;/i&gt; is common. However, there is limited data on the biochemical profiles of patients coinfected with malaria and &lt;i&gt;S. mansoni&lt;/i&gt; schistosomiasis in the study area. Hence, this study aimed to assess the effect of malaria and &lt;i&gt;S. mansoni&lt;/i&gt; schistosomiasis coinfection on selected biochemical profiles. &lt;i&gt;Methods&lt;/i&gt;. An institutional-based comparative cross-sectional study was conducted from March 30 to August 10, 2022. Using a convenient sampling technique, 70 participants (35 cases and 35 controls) were enrolled in the study. &lt;i&gt;Schistosoma mansoni&lt;/i&gt; was detected in stool samples using the wet mount and the Kato Katz method. To detect &lt;i&gt;Plasmodium&lt;/i&gt;, both thick and thin blood films were prepared and stained with Giemsa. The blood sample was processed for the analysis of biochemical profiles. All data were analyzed using SPSS version 25. A &lt;svg height=\"10.2124pt\" style=\"vertical-align:-3.42943pt\" version=\"1.1\" viewbox=\"-0.0498162 -6.78297 7.83752 10.2124\" width=\"7.83752pt\" xmlns=\"http://www.w3.org/2000/svg\" xmlns:xlink=\"http://www.w3.org/1999/xlink\"&gt;&lt;g transform=\"matrix(.013,0,0,-0.013,0,0)\"&gt;&lt;/path&gt;&lt;/g&gt;&lt;/svg&gt; value of less than 0.05 was considered statistically significant. &lt;i&gt;Results&lt;/i&gt;. The mean values of alanine aminotransferase and aspartate aminotransferase (37.1 U/L and 41.9 U/L, respectively) in coinfected participants were significantly higher than in the healthy control participants (17.4 U/L and 22.0 U/L, respectively) &lt;span&gt;&lt;svg height=\"12.7178pt\" style=\"vertical-align:-3.42947pt\" version=\"1.1\" viewbox=\"-0.0498162 -9.28833 23.471 12.7178\" width=\"23.471pt\" xmlns=\"http://www.w3.org/2000/svg\" xmlns:xlink=\"http://www.w3.org/1999/xlink\"&gt;&lt;g transform=\"matrix(.013,0,0,-0.013,0,0)\"&gt;&lt;/path&gt;&lt;/g&gt;&lt;g transform=\"matrix(.013,0,0,-0.013,4.498,0)\"&gt;&lt;use xlink:href=\"#g113-113\"&gt;&lt;/use&gt;&lt;/g&gt;&lt;g transform=\"matrix(.013,0,0,-0.013,15.84,0)\"&gt;&lt;/path&gt;&lt;/g&gt;&lt;/svg&gt;&lt;span&gt;&lt;/span&gt;&lt;span&gt;&lt;svg height=\"12.7178pt\" style=\"vertical-align:-3.42947pt\" version=\"1.1\" viewbox=\"27.053183800000003 -9.28833 26.453 12.7178\" width=\"26.453pt\" xmlns=\"http://www.w3.org/2000/svg\" xmlns:xlink=\"http://www.w3.org/1999/xlink\"&gt;&lt;g transform=\"matrix(.013,0,0,-0.013,27.103,0)\"&gt;&lt;/path&gt;&lt;/g&gt;&lt;g transform=\"matrix(.013,0,0,-0.013,33.343,0)\"&gt;&lt;/path&gt;&lt;/g&gt;&lt;g transform=\"matrix(.013,0,0,-0.013,36.307,0)\"&gt;&lt;use xlink:href=\"#g113-49\"&gt;&lt;/use&gt;&lt;/g&gt;&lt;g transform=\"matrix(.013,0,0,-0.013,42.547,0)\"&gt;&lt;/path&gt;&lt;/g&gt;&lt;g transform=\"matrix(.013,0,0,-0.013,48.787,0)\"&gt;&lt;/path&gt;&lt;/g&gt;&lt;/svg&gt;.&lt;/span&gt;&lt;/span&gt; Also, the median values of creatinine, total bilirubin, and direct bilirubin (1.51 mg/dL, 2.35 mg/dL, and 0.91 mg/dL, respectively) in coinfected participants were significantly higher than in the healthy control participants ","PeriodicalId":17527,"journal":{"name":"Journal of Tropical Medicine","volume":"50 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140201024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
In Vitro Antischistosomal Activity of Bridelia ferruginea, Clausena anisata, Khaya senegalensis, and Vernonia amygdalina Bridelia ferruginea、Clausena anisata、Khaya senegalensis 和 Vernonia amygdalina 的体外抗血吸虫活性
IF 2.2 4区 医学 Q3 PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH Pub Date : 2024-03-21 DOI: 10.1155/2024/8074291
Deryl Nii Okantey Kuevi, Jennifer Keiser, Cécile Häberli, Abena Konadu Owusu-Senyah, Mawutor Kwame Ahiabu
Background. Schistosomiasis is caused by parasitic flatworms and the disease is endemic to most countries in sub-Saharan Africa including Ghana. The current therapeutic agent for managing this disease solely relies on praziquantel. The continual dependence on this single available drug could lead to possible drug resistance. This study seeks to evaluate the antischistosomal activity of the following Ghanaian medicinal plants: Khaya senegalensis, Vernonia amygdalina, Clausena anisata, and Bridelia ferruginea. Methodology. Two concentrations (100 μg/mL and 50 μg/mL) of each extract were tested in a 96-well plate containing 30 newly transformed schistosomula (NTS). Moreover, six worms of both sexes of adult Schistosoma mansoni were exposed to the extracts diluted in the RPMI medium. The assay was performed in a 24-well plate. The parasitic worms were examined using an inverted optical microscope. Results. At 100 μg/mL and 50 μg/mL, all extracts performed better and showed strong activity () against NTS; thus, 98.08%, 100%, 80.77%, and 100% for Clausena, Vernonia, Bridelia, and Khaya, respectively, when compared to praziquantel. Strong activity was recorded when the extracts underwent testing against Schistosoma mansoni adults at 100 μg/mL; 96.35%, 100%, and 94.55% for Vernonia, Bridelia, and Khaya, respectively, except for Clausena which exhibited weak activity, i.e., 56.02%. There was no significant difference between Vernonia, Bridelia, and Khaya when compared to praziquantel. Conclusion. At 100 μg/mL, Khaya senegalensis, Vernonia amygdalina, and Bridelia ferruginea extracts demonstrated strong activity against both schistosomula and adult Schistosoma mansoni. These data can serve as baseline information in the quest to find alternative therapeutic agents to treat schistosomiasis.
背景。血吸虫病由扁形寄生虫引起,在包括加纳在内的撒哈拉以南非洲大多数国家流行。目前治疗这种疾病的药物主要是吡喹酮。对这种单一药物的持续依赖可能会导致耐药性。本研究旨在评估以下加纳药用植物的抗吸虫活性:Khaya senegalensis、Vernonia amygdalina、Clausena anisata 和 Bridelia ferruginea。研究方法。在含有 30 只新转化血吸虫(NTS)的 96 孔板中测试每种提取物的两种浓度(100 μg/mL 和 50 μg/mL)。此外,曼氏血吸虫成虫的 6 只雌雄虫也暴露于稀释在 RPMI 培养基中的提取物。试验在 24 孔板中进行。使用倒置光学显微镜检查寄生虫。结果在100 μg/mL和50 μg/mL浓度下,所有提取物对NTS都有较好的活性,与吡喹酮相比,Clausena、Vernonia、Bridelia和Khaya的活性分别为98.08%、100%、80.77%和100%。在对曼氏血吸虫成虫进行 100 μg/mL 的测试时,Vernonia、Bridelia 和 Khaya 的提取物具有很强的活性,分别为 96.35%、100% 和 94.55%,只有 Clausena 的活性较弱,仅为 56.02%。与吡喹酮相比,Vernonia、Bridelia 和 Khaya 没有明显差异。结论在 100 μg/mL 的浓度下,Khaya senegalensis、Vernonia amygdalina 和 Bridelia ferruginea 提取物对血吸虫和曼氏血吸虫成虫都有很强的活性。这些数据可作为寻找治疗血吸虫病的替代药物的基础信息。
{"title":"In Vitro Antischistosomal Activity of Bridelia ferruginea, Clausena anisata, Khaya senegalensis, and Vernonia amygdalina","authors":"Deryl Nii Okantey Kuevi, Jennifer Keiser, Cécile Häberli, Abena Konadu Owusu-Senyah, Mawutor Kwame Ahiabu","doi":"10.1155/2024/8074291","DOIUrl":"https://doi.org/10.1155/2024/8074291","url":null,"abstract":"<i>Background</i>. Schistosomiasis is caused by parasitic flatworms and the disease is endemic to most countries in sub-Saharan Africa including Ghana. The current therapeutic agent for managing this disease solely relies on praziquantel. The continual dependence on this single available drug could lead to possible drug resistance. This study seeks to evaluate the antischistosomal activity of the following Ghanaian medicinal plants: <i>Khaya senegalensis</i>, <i>Vernonia amygdalina</i>, <i>Clausena anisata</i>, and <i>Bridelia ferruginea. Methodology</i>. Two concentrations (100 <i>μ</i>g/mL and 50 <i>μ</i>g/mL) of each extract were tested in a 96-well plate containing 30 newly transformed schistosomula (NTS). Moreover, six worms of both sexes of adult <i>Schistosoma mansoni</i> were exposed to the extracts diluted in the RPMI medium. The assay was performed in a 24-well plate. The parasitic worms were examined using an inverted optical microscope. <i>Results</i>. At 100 <i>μ</i>g/mL and 50 <i>μ</i>g/mL, all extracts performed better and showed strong activity (<span><svg height=\"11.7782pt\" style=\"vertical-align:-3.42938pt\" version=\"1.1\" viewbox=\"-0.0498162 -8.34882 18.973 11.7782\" width=\"18.973pt\" xmlns=\"http://www.w3.org/2000/svg\" xmlns:xlink=\"http://www.w3.org/1999/xlink\"><g transform=\"matrix(.013,0,0,-0.013,0,0)\"></path></g><g transform=\"matrix(.013,0,0,-0.013,11.342,0)\"></path></g></svg><span></span><span><svg height=\"11.7782pt\" style=\"vertical-align:-3.42938pt\" version=\"1.1\" viewbox=\"22.555183800000002 -8.34882 28.184 11.7782\" width=\"28.184pt\" xmlns=\"http://www.w3.org/2000/svg\" xmlns:xlink=\"http://www.w3.org/1999/xlink\"><g transform=\"matrix(.013,0,0,-0.013,22.605,0)\"></path></g><g transform=\"matrix(.013,0,0,-0.013,28.845,0)\"></path></g><g transform=\"matrix(.013,0,0,-0.013,31.809,0)\"><use xlink:href=\"#g113-49\"></use></g><g transform=\"matrix(.013,0,0,-0.013,38.049,0)\"><use xlink:href=\"#g113-49\"></use></g><g transform=\"matrix(.013,0,0,-0.013,44.289,0)\"></path></g></svg>)</span></span> against NTS; thus, 98.08%, 100%, 80.77%, and 100% for Clausena, Vernonia, Bridelia, and Khaya, respectively, when compared to praziquantel. Strong activity was recorded when the extracts underwent testing against <i>Schistosoma mansoni</i> adults at 100 <i>μ</i>g/mL; 96.35%, 100%, and 94.55% for Vernonia, Bridelia, and Khaya, respectively, except for Clausena which exhibited weak activity, i.e., 56.02%. There was no significant difference between Vernonia, Bridelia, and Khaya when compared to praziquantel. <i>Conclusion</i>. At 100 <i>μ</i>g/mL, <i>Khaya senegalensis</i>, <i>Vernonia amygdalina</i>, and <i>Bridelia ferruginea</i> extracts demonstrated strong activity against both schistosomula and adult <i>Schistosoma mansoni</i>. These data can serve as baseline information in the quest to find alternative therapeutic agents to treat schistosomiasis.","PeriodicalId":17527,"journal":{"name":"Journal of Tropical Medicine","volume":"79 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140201105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
MicroRNA-1 Inhibits the Growth of Breast Cancer Cells MDA-MB-231 and MCF-7 Treated with Hydatid Cyst Fluid MicroRNA-1 可抑制经水瘤囊液处理的乳腺癌细胞 MDA-MB-231 和 MCF-7 的生长
IF 2.2 4区 医学 Q3 PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH Pub Date : 2024-03-12 DOI: 10.1155/2024/7474039
Hadis Jafari, Mahmoud Mahami-Oskouei, Adel Spotin, Behzad Baradaran, Dariush Shanehbandi, Amir Baghbanzadeh, Zahra Alizadeh
Antigens in hydatid cyst fluid (HCF) have been discovered to bear a significant resemblance to antigens present in cancer cells. MicroRNA-1 (miR-1) is a well-known member of the tumor inhibitor miRNA family and has been shown to have pro-apoptotic and tumor-inhibitory functions. This study aimed to evaluate the ability of HCF to prevent breast cancer and to explore the underlying mechanisms that affect cancer cells. For this study, MDA-MB-231 and MCF-7 breast cancer cells were cultured and divided into two groups: one group received HCF treatment and the other group was untreated and served as the control group. The cytotoxicity and cell viability of various HCF concentrations on breast cancer cells were evaluated using the MTT assay. In addition, the expression level of miR-1 in HCF-treated and untreated breast cancer cells was analyzed using qRT-PCR. The study found that HCF treatment reduced the growth of MDA-MB-231 and MCF-7 breast cancer cells, indicating that it was cytotoxic to the cells. Specifically, the IC50 concentration of HCF after 24 hours of treatment was 7.32 <i>µ</i>g/mL for MDA-MB-231 cells and 13.63 <i>µ</i>g/mL for MCF-7 cells. In addition, qRT-PCR analysis revealed that the expression level of miR-1 was significantly increased in HCF-treated MDA-MB-231 (<span><svg height="8.8423pt" style="vertical-align:-0.2064009pt" version="1.1" viewbox="-0.0498162 -8.6359 19.289 8.8423" width="19.289pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"></path></g><g transform="matrix(.013,0,0,-0.013,11.658,0)"></path></g></svg><span></span><span><svg height="8.8423pt" style="vertical-align:-0.2064009pt" version="1.1" viewbox="22.8711838 -8.6359 34.445 8.8423" width="34.445pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,22.921,0)"></path></g><g transform="matrix(.013,0,0,-0.013,29.161,0)"></path></g><g transform="matrix(.013,0,0,-0.013,32.125,0)"><use xlink:href="#g113-49"></use></g><g transform="matrix(.013,0,0,-0.013,38.365,0)"></path></g><g transform="matrix(.013,0,0,-0.013,44.605,0)"><use xlink:href="#g113-49"></use></g><g transform="matrix(.013,0,0,-0.013,50.845,0)"></path></g></svg>)</span></span> and MCF-7 (<span><svg height="8.8423pt" style="vertical-align:-0.2064009pt" version="1.1" viewbox="-0.0498162 -8.6359 19.289 8.8423" width="19.289pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><use xlink:href="#g113-81"></use></g><g transform="matrix(.013,0,0,-0.013,11.658,0)"><use xlink:href="#g117-34"></use></g></svg><span></span><span><svg height="8.8423pt" style="vertical-align:-0.2064009pt" version="1.1" viewbox="22.8711838 -8.6359 34.445 8.8423" width="34.445pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,22.921,0)"><use xlink:href="#g113-49"></use><
研究发现,包虫囊肿液(HCF)中的抗原与癌细胞中的抗原非常相似。微RNA-1(miR-1)是众所周知的肿瘤抑制剂miRNA家族成员,已被证明具有促凋亡和抑制肿瘤的功能。本研究旨在评估 HCF 预防乳腺癌的能力,并探索影响癌细胞的潜在机制。在本研究中,培养了 MDA-MB-231 和 MCF-7 乳腺癌细胞,并将其分为两组:一组接受 HCF 处理,另一组未经处理,作为对照组。采用 MTT 法评估不同浓度的 HCF 对乳腺癌细胞的细胞毒性和细胞活力。此外,还使用 qRT-PCR 分析了经 HCF 处理和未处理的乳腺癌细胞中 miR-1 的表达水平。研究发现,HCF 处理可降低 MDA-MB-231 和 MCF-7 乳腺癌细胞的生长,表明它对细胞具有细胞毒性。具体来说,处理 24 小时后,HCF 对 MDA-MB-231 细胞的 IC50 浓度为 7.32 微克/毫升,对 MCF-7 细胞的 IC50 浓度为 13.63 微克/毫升。此外,qRT-PCR分析显示,与未处理的对照组相比,经HCF处理的MDA-MB-231()和MCF-7()细胞系中miR-1的表达水平显著增加。尽管研究表明 HCF 可抑制乳腺癌细胞的生长,并上调 miR-1(癌细胞中的一种关键肿瘤抑制因子),但这种作用的具体机制仍不清楚。要全面了解 HCF 抗肿瘤活性的分子途径及其作为癌症治疗剂的潜力,还需要进一步的研究。
{"title":"MicroRNA-1 Inhibits the Growth of Breast Cancer Cells MDA-MB-231 and MCF-7 Treated with Hydatid Cyst Fluid","authors":"Hadis Jafari, Mahmoud Mahami-Oskouei, Adel Spotin, Behzad Baradaran, Dariush Shanehbandi, Amir Baghbanzadeh, Zahra Alizadeh","doi":"10.1155/2024/7474039","DOIUrl":"https://doi.org/10.1155/2024/7474039","url":null,"abstract":"Antigens in hydatid cyst fluid (HCF) have been discovered to bear a significant resemblance to antigens present in cancer cells. MicroRNA-1 (miR-1) is a well-known member of the tumor inhibitor miRNA family and has been shown to have pro-apoptotic and tumor-inhibitory functions. This study aimed to evaluate the ability of HCF to prevent breast cancer and to explore the underlying mechanisms that affect cancer cells. For this study, MDA-MB-231 and MCF-7 breast cancer cells were cultured and divided into two groups: one group received HCF treatment and the other group was untreated and served as the control group. The cytotoxicity and cell viability of various HCF concentrations on breast cancer cells were evaluated using the MTT assay. In addition, the expression level of miR-1 in HCF-treated and untreated breast cancer cells was analyzed using qRT-PCR. The study found that HCF treatment reduced the growth of MDA-MB-231 and MCF-7 breast cancer cells, indicating that it was cytotoxic to the cells. Specifically, the IC50 concentration of HCF after 24 hours of treatment was 7.32 &lt;i&gt;µ&lt;/i&gt;g/mL for MDA-MB-231 cells and 13.63 &lt;i&gt;µ&lt;/i&gt;g/mL for MCF-7 cells. In addition, qRT-PCR analysis revealed that the expression level of miR-1 was significantly increased in HCF-treated MDA-MB-231 (&lt;span&gt;&lt;svg height=\"8.8423pt\" style=\"vertical-align:-0.2064009pt\" version=\"1.1\" viewbox=\"-0.0498162 -8.6359 19.289 8.8423\" width=\"19.289pt\" xmlns=\"http://www.w3.org/2000/svg\" xmlns:xlink=\"http://www.w3.org/1999/xlink\"&gt;&lt;g transform=\"matrix(.013,0,0,-0.013,0,0)\"&gt;&lt;/path&gt;&lt;/g&gt;&lt;g transform=\"matrix(.013,0,0,-0.013,11.658,0)\"&gt;&lt;/path&gt;&lt;/g&gt;&lt;/svg&gt;&lt;span&gt;&lt;/span&gt;&lt;span&gt;&lt;svg height=\"8.8423pt\" style=\"vertical-align:-0.2064009pt\" version=\"1.1\" viewbox=\"22.8711838 -8.6359 34.445 8.8423\" width=\"34.445pt\" xmlns=\"http://www.w3.org/2000/svg\" xmlns:xlink=\"http://www.w3.org/1999/xlink\"&gt;&lt;g transform=\"matrix(.013,0,0,-0.013,22.921,0)\"&gt;&lt;/path&gt;&lt;/g&gt;&lt;g transform=\"matrix(.013,0,0,-0.013,29.161,0)\"&gt;&lt;/path&gt;&lt;/g&gt;&lt;g transform=\"matrix(.013,0,0,-0.013,32.125,0)\"&gt;&lt;use xlink:href=\"#g113-49\"&gt;&lt;/use&gt;&lt;/g&gt;&lt;g transform=\"matrix(.013,0,0,-0.013,38.365,0)\"&gt;&lt;/path&gt;&lt;/g&gt;&lt;g transform=\"matrix(.013,0,0,-0.013,44.605,0)\"&gt;&lt;use xlink:href=\"#g113-49\"&gt;&lt;/use&gt;&lt;/g&gt;&lt;g transform=\"matrix(.013,0,0,-0.013,50.845,0)\"&gt;&lt;/path&gt;&lt;/g&gt;&lt;/svg&gt;)&lt;/span&gt;&lt;/span&gt; and MCF-7 (&lt;span&gt;&lt;svg height=\"8.8423pt\" style=\"vertical-align:-0.2064009pt\" version=\"1.1\" viewbox=\"-0.0498162 -8.6359 19.289 8.8423\" width=\"19.289pt\" xmlns=\"http://www.w3.org/2000/svg\" xmlns:xlink=\"http://www.w3.org/1999/xlink\"&gt;&lt;g transform=\"matrix(.013,0,0,-0.013,0,0)\"&gt;&lt;use xlink:href=\"#g113-81\"&gt;&lt;/use&gt;&lt;/g&gt;&lt;g transform=\"matrix(.013,0,0,-0.013,11.658,0)\"&gt;&lt;use xlink:href=\"#g117-34\"&gt;&lt;/use&gt;&lt;/g&gt;&lt;/svg&gt;&lt;span&gt;&lt;/span&gt;&lt;span&gt;&lt;svg height=\"8.8423pt\" style=\"vertical-align:-0.2064009pt\" version=\"1.1\" viewbox=\"22.8711838 -8.6359 34.445 8.8423\" width=\"34.445pt\" xmlns=\"http://www.w3.org/2000/svg\" xmlns:xlink=\"http://www.w3.org/1999/xlink\"&gt;&lt;g transform=\"matrix(.013,0,0,-0.013,22.921,0)\"&gt;&lt;use xlink:href=\"#g113-49\"&gt;&lt;/use&gt;&lt;","PeriodicalId":17527,"journal":{"name":"Journal of Tropical Medicine","volume":"38 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140106911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Antibacterial and Antioxidant Efficacies of Secondary Metabolites from the Roots of Cyphostemma adenocaule: A Combined In Vitro and In Silico Study Cyphostemma adenocaule 根部次生代谢物的抗菌和抗氧化功效:体外和硅学联合研究
IF 2.2 4区 医学 Q3 PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH Pub Date : 2024-03-06 DOI: 10.1155/2024/1679695
Hadush Gebrehiwot, Yadessa Melaku, Muhdin Aliye, Urgessa Ensermu, Aman Dekebo, Milkyas Endale, Daniel Rentsch, Mo Hunsen
Cyphostemma adenocaule is a therapeutic plant traditionally used to treat rabies, snake bite, diarrhea, and wound healing. To address the bioactive compounds exhibiting these activities, we performed a comprehensive study on the roots of the plant. Thus, the present study aims to inspect the in vitro antioxidant and antibacterial efficacies of compounds isolated from the combined dichloromethane : methanol (1 : 1) and methanol extracts of C. adenocaule along with the in silico study of their interaction with selected protein targets. The silica gel column chromatography technique was used for the isolation of compounds, and the antibacterial and antioxidant activities were evaluated using agar disc diffusion and DPPH radical scavenging assays, respectively. Furthermore, in silico molecular docking screening, pharmacokinetics, and toxicity protocols of the compound isolates were performed to offer the potential applications of the compounds in developing novel medications. A BIOVIA Discovery Studio in combination with AutoDock Vina 4.2 software, SwissADME, and ProTox-II prediction web tools were used to generate the molecular docking, pharmacokinetics, and toxicity profiles, respectively. Notably, the chromatographic separation of the combined extracts yielded six known compounds, namely, β-sitosterol (1), 3-hydroxyisoagatholactone (2), ε-viniferin (3), myricetin (4), tricuspidatol A (5), and parthenocissin A (6). The in vitro antibacterial activities revealed the highest inhibition zone by tricuspidatol A (5) (16.67 ± 0.47), showcasing its potent activity against S. aureus at 2 mg/mL, compared to ciprofloxacin (21.50 ± 0.41). ε-Viniferin (3) (IC50: 0.32 μg/mL) exhibited greater antioxidant activity than the others and displayed promising results compared to ascorbic acid (0.075 μg/mL). The molecular docking study revealed the highest binding affinity by ε-viniferin (3) (−9.9 kcal/mol) against topoisomerase II α. 3-Hydroxyisoagatholactone (2) and ε-viniferin (3) fulfilled Lipinski’s rule with no violation, and the organ toxicity predictions revealed that all the compounds showed no cytotoxicity and hepatotoxicity effects. Thus, this study’s combined in vitro and in silico outcomes suggest the potential use of the isolated compounds in drug discovery and support the traditional relevance of C. adenocaule.
Cyphostemma adenocaule 是一种治疗植物,传统上用于治疗狂犬病、蛇咬伤、腹泻和伤口愈合。为了研究具有这些活性的生物活性化合物,我们对该植物的根部进行了全面研究。因此,本研究旨在检测从 C. adenocaule 的二氯甲烷:甲醇(1:1)和甲醇联合提取物中分离出来的化合物的体外抗氧化和抗菌功效,以及它们与选定蛋白质靶标相互作用的硅学研究。采用硅胶柱层析技术分离化合物,并分别使用琼脂盘扩散法和 DPPH 自由基清除法评估其抗菌和抗氧化活性。此外,还对分离出的化合物进行了分子对接筛选、药代动力学和毒性分析,以提供这些化合物在开发新型药物方面的潜在应用。BIOVIA Discovery Studio结合AutoDock Vina 4.2软件、SwissADME和ProTox-II预测网络工具分别生成了分子对接、药代动力学和毒性图谱。值得注意的是,在对组合提取物进行色谱分离时,得到了六个已知化合物,即β-谷甾醇(1)、3-羟基异泽泻内酯(2)、ε-维尼芬素(3)、三尖杉酯素(4)、三尖杉醇 A(5)和半知母苷 A(6)。体外抗菌活性显示,与环丙沙星(21.50 ± 0.41)相比,三尖杉酯醇 A(5)的抑菌区最大(16.67 ± 0.47),表明其在 2 毫克/毫升浓度下对金黄色葡萄球菌具有强效活性。ε-Viniferin (3) (IC50:0.32 μg/mL)的抗氧化活性高于其他化合物,与抗坏血酸(0.075 μg/mL)相比,显示出良好的效果。分子对接研究表明,ε-葡萄素(3)与拓扑异构酶 II α的结合亲和力最高(-9.9 kcal/mol)。因此,本研究的体外和硅学综合结果表明,分离出的化合物有可能被用于药物研发,并支持了C. adenocaule的传统相关性。
{"title":"Antibacterial and Antioxidant Efficacies of Secondary Metabolites from the Roots of Cyphostemma adenocaule: A Combined In Vitro and In Silico Study","authors":"Hadush Gebrehiwot, Yadessa Melaku, Muhdin Aliye, Urgessa Ensermu, Aman Dekebo, Milkyas Endale, Daniel Rentsch, Mo Hunsen","doi":"10.1155/2024/1679695","DOIUrl":"https://doi.org/10.1155/2024/1679695","url":null,"abstract":"<i>Cyphostemma adenocaule</i> is a therapeutic plant traditionally used to treat rabies, snake bite, diarrhea, and wound healing. To address the bioactive compounds exhibiting these activities, we performed a comprehensive study on the roots of the plant. Thus, the present study aims to inspect the <i>in vitro</i> antioxidant and antibacterial efficacies of compounds isolated from the combined dichloromethane : methanol (1 : 1) and methanol extracts of <i>C. adenocaule</i> along with the <i>in silico</i> study of their interaction with selected protein targets. The silica gel column chromatography technique was used for the isolation of compounds, and the antibacterial and antioxidant activities were evaluated using agar disc diffusion and DPPH radical scavenging assays, respectively. Furthermore, <i>in silico</i> molecular docking screening, pharmacokinetics, and toxicity protocols of the compound isolates were performed to offer the potential applications of the compounds in developing novel medications. A BIOVIA Discovery Studio in combination with AutoDock Vina 4.2 software, SwissADME, and ProTox-II prediction web tools were used to generate the molecular docking, pharmacokinetics, and toxicity profiles, respectively. Notably, the chromatographic separation of the combined extracts yielded six known compounds, namely, <i>β-</i>sitosterol (<b>1</b>), 3-hydroxyisoagatholactone (<b>2</b>), <i>ε</i>-viniferin (<b>3</b>), myricetin (<b>4</b>), tricuspidatol A (<b>5</b>), and parthenocissin A (<b>6</b>). The <i>in vitro</i> antibacterial activities revealed the highest inhibition zone by tricuspidatol A (<b>5</b>) (16.67 ± 0.47), showcasing its potent activity against <i>S. aureus</i> at 2 mg/mL, compared to ciprofloxacin (21.50 ± 0.41). <i>ε</i>-Viniferin (<b>3</b>) (IC<sub>50</sub>: 0.32 <i>μ</i>g/mL) exhibited greater antioxidant activity than the others and displayed promising results compared to ascorbic acid (0.075 <i>μ</i>g/mL). The molecular docking study revealed the highest binding affinity by <i>ε-</i>viniferin (<b>3</b>) (−9.9 kcal/mol) against topoisomerase II <i>α.</i> 3-Hydroxyisoagatholactone (<b>2</b>) and <i>ε</i>-viniferin (<b>3</b>) fulfilled Lipinski’s rule with no violation, and the organ toxicity predictions revealed that all the compounds showed no cytotoxicity and hepatotoxicity effects. Thus, this study’s combined <i>in vitro</i> and <i>in silico</i> outcomes suggest the potential use of the isolated compounds in drug discovery and support the traditional relevance of <i>C. adenocaule</i>.","PeriodicalId":17527,"journal":{"name":"Journal of Tropical Medicine","volume":"34 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140047011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The Malaria Burden: A South African Perspective 疟疾负担:南非视角
IF 2.2 4区 医学 Q3 PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH Pub Date : 2024-02-23 DOI: 10.1155/2024/6619010
Marissa Balmith, Charlise Basson, Sarel J. Brand
Malaria is a deadly disease caused by protozoan pathogens of the Plasmodium parasite. Transmission to humans occurs through the bite of an infected female Anopheles mosquito. According to the World Health Organization (WHO), an estimated 247 million cases of malaria were recorded worldwide in 2021, with approximately 619 000 malaria deaths. The initial signs of malaria can be mild and challenging to diagnose due to the signs and symptoms being similar to those of other illnesses. The malaria burden remains largely concentrated in the WHO sub-Saharan African region and has been recognised as a significant contributor to morbidity and mortality. This review aims to contribute to the existing knowledge on malaria in South Africa, a region within sub-Saharan Africa, focusing on the epidemiology and life cycle of the malaria parasite as well as diagnostic approaches for detecting malaria. In addition, nonpharmacological and pharmacological interventions for treating and preventing malaria infections will also be discussed herein. While there has been a significant reduction in the global burden of this disease, malaria remains a public health issue in South Africa. As such, the implementation of effective preventative measures and strategies, early diagnosis, and appropriate treatment regimens are crucial to reducing the malaria burden in South Africa.
疟疾是一种由疟原虫病原体引起的致命疾病。疟疾通过受感染的雌性按蚊叮咬传播给人类。据世界卫生组织(WHO)估计,2021 年全球疟疾病例约为 2.47 亿例,约有 619 000 人死于疟疾。疟疾的初期症状可能比较轻微,而且由于症状和体征与其他疾病相似,因此很难诊断。疟疾负担仍主要集中在世卫组织撒哈拉以南非洲地区,已被公认为发病率和死亡率的重要因素。本综述旨在为撒哈拉以南非洲地区的南非提供有关疟疾的现有知识,重点关注流行病学、疟原虫的生命周期以及检测疟疾的诊断方法。此外,本文还将讨论治疗和预防疟疾感染的非药物和药物干预措施。虽然这一疾病在全球造成的负担已大大减轻,但疟疾仍然是南非的一个公共卫生问题。因此,实施有效的预防措施和战略、早期诊断和适当的治疗方案对于减轻南非的疟疾负担至关重要。
{"title":"The Malaria Burden: A South African Perspective","authors":"Marissa Balmith, Charlise Basson, Sarel J. Brand","doi":"10.1155/2024/6619010","DOIUrl":"https://doi.org/10.1155/2024/6619010","url":null,"abstract":"Malaria is a deadly disease caused by protozoan pathogens of the <i>Plasmodium</i> parasite. Transmission to humans occurs through the bite of an infected female <i>Anopheles</i> mosquito. According to the World Health Organization (WHO), an estimated 247 million cases of malaria were recorded worldwide in 2021, with approximately 619 000 malaria deaths. The initial signs of malaria can be mild and challenging to diagnose due to the signs and symptoms being similar to those of other illnesses. The malaria burden remains largely concentrated in the WHO sub-Saharan African region and has been recognised as a significant contributor to morbidity and mortality. This review aims to contribute to the existing knowledge on malaria in South Africa, a region within sub-Saharan Africa, focusing on the epidemiology and life cycle of the malaria parasite as well as diagnostic approaches for detecting malaria. In addition, nonpharmacological and pharmacological interventions for treating and preventing malaria infections will also be discussed herein. While there has been a significant reduction in the global burden of this disease, malaria remains a public health issue in South Africa. As such, the implementation of effective preventative measures and strategies, early diagnosis, and appropriate treatment regimens are crucial to reducing the malaria burden in South Africa.","PeriodicalId":17527,"journal":{"name":"Journal of Tropical Medicine","volume":"44 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139955414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Systematic Review and Meta-Analysis of Congenital Toxoplasmosis Diagnosis: Advances and Challenges 先天性弓形虫病诊断的系统回顾和元分析:进展与挑战
IF 2.2 4区 医学 Q3 PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH Pub Date : 2024-02-21 DOI: 10.1155/2024/1514178
Priscila Silva Franco, Ana Carolina Morais Oliveira Scussel, Rafaela José Silva, Thadia Evelyn Araújo, Henrique Tomaz Gonzaga, Camila Ferreira Marcon, Joaquim Pedro Brito-de-Sousa, Angélica Lemos Debs Diniz, Marina Carvalho Paschoini, Bellisa Freitas Barbosa, Olindo Assis Martins-Filho, José Roberto Mineo, Eloisa Amália Vieira Ferro, Angelica Oliveira Gomes
Objective. To understand how congenital toxoplasmosis (CT) diagnosis has evolved over the years, we performed a systematic review and meta-analysis to summarize the kind of analysis that has been employed for CT diagnosis. Methods. PubMed and Lilacs databases were used in order to access the kind of analysis that has been employed for CT diagnosis in several samples. Our search combined the following combining terms: “congenital toxoplasmosis” or “gestational toxoplasmosis” and “diagnosis” and “blood,” “serum,” “amniotic fluid,” “placenta,” or “colostrum.” We extracted data on true positive, true negative, false positive, and false negative to generate pooled sensitivity, specificity, and diagnostic odds ratio (DOR). Random-effects models using MetaDTA were used for analysis. Results. Sixty-five articles were included in the study aiming for comparisons (75.4%), diagnosis performance (52.3%), diagnosis improvement (32.3%), or to distinguish acute/chronic infection phases (36.9%). Amniotic fluid (AF) and placenta were used in 36.9% and 10.8% of articles, respectively, targeting parasites and/or T. gondii DNA. Blood was used in 86% of articles for enzymatic assays. Colostrum was used in one article to search for antibodies. In meta-analysis, PCR in AF showed the best performance for CT diagnosis based on the highest summary sensitivity (85.1%) and specificity (99.7%) added to lower magnitude heterogeneity. Conclusion. Most of the assays being researched to diagnose CT are basically the same traditional approaches available for clinical purposes. The range in diagnostic performance and the challenges imposed by CT diagnosis indicate the need to better explore pregnancy samples in search of new possibilities for diagnostic tools. Exploring immunological markers and using bioinformatics tools and T. gondii recombinant antigens should address the research needed for a new generation of diagnostic tools to face these challenges.
目的。为了解先天性弓形虫病(CT)诊断多年来的发展情况,我们进行了一项系统回顾和荟萃分析,总结了 CT 诊断所采用的分析方法。方法:PubMed 和 Lilacs我们使用了 PubMed 和 Lilacs 数据库,以获取多个样本中用于 CT 诊断的分析方法。我们的搜索结合了以下组合词:"先天性弓形虫病 "或 "妊娠弓形虫病"、"诊断"、"血液"、"血清"、"羊水"、"胎盘 "或 "初乳"。我们提取了真阳性、真阴性、假阳性和假阴性的数据,以生成集合灵敏度、特异性和诊断几率比(DOR)。使用 MetaDTA 随机效应模型进行分析。结果。研究共纳入 65 篇文章,目的是进行比较(75.4%)、诊断性能(52.3%)、诊断改进(32.3%)或区分急性/慢性感染阶段(36.9%)。分别有 36.9% 和 10.8% 的文章使用羊水和胎盘检测寄生虫和/或淋球菌 DNA。86%的文章使用血液进行酶测定。一篇文章使用牛初乳来寻找抗体。在荟萃分析中,AF 中的 PCR 在 CT 诊断中表现最佳,灵敏度(85.1%)和特异度(99.7%)均最高,异质性较低。结论目前研究的大多数 CT 诊断方法基本上都是用于临床的传统方法。CT 诊断所带来的诊断性能差异和挑战表明,有必要更好地探索妊娠样本,寻找新的诊断工具。探索免疫标记物、使用生物信息学工具和淋病双球菌重组抗原应能解决新一代诊断工具所需的研究,以应对这些挑战。
{"title":"Systematic Review and Meta-Analysis of Congenital Toxoplasmosis Diagnosis: Advances and Challenges","authors":"Priscila Silva Franco, Ana Carolina Morais Oliveira Scussel, Rafaela José Silva, Thadia Evelyn Araújo, Henrique Tomaz Gonzaga, Camila Ferreira Marcon, Joaquim Pedro Brito-de-Sousa, Angélica Lemos Debs Diniz, Marina Carvalho Paschoini, Bellisa Freitas Barbosa, Olindo Assis Martins-Filho, José Roberto Mineo, Eloisa Amália Vieira Ferro, Angelica Oliveira Gomes","doi":"10.1155/2024/1514178","DOIUrl":"https://doi.org/10.1155/2024/1514178","url":null,"abstract":"<i>Objective</i>. To understand how congenital toxoplasmosis (CT) diagnosis has evolved over the years, we performed a systematic review and meta-analysis to summarize the kind of analysis that has been employed for CT diagnosis. <i>Methods</i>. PubMed and Lilacs databases were used in order to access the kind of analysis that has been employed for CT diagnosis in several samples. Our search combined the following combining terms: “congenital toxoplasmosis” or “gestational toxoplasmosis” and “diagnosis” and “blood,” “serum,” “amniotic fluid,” “placenta,” or “colostrum.” We extracted data on true positive, true negative, false positive, and false negative to generate pooled sensitivity, specificity, and diagnostic odds ratio (DOR). Random-effects models using MetaDTA were used for analysis. <i>Results</i>. Sixty-five articles were included in the study aiming for comparisons (75.4%), diagnosis performance (52.3%), diagnosis improvement (32.3%), or to distinguish acute/chronic infection phases (36.9%). Amniotic fluid (AF) and placenta were used in 36.9% and 10.8% of articles, respectively, targeting parasites and/or <i>T. gondii</i> DNA. Blood was used in 86% of articles for enzymatic assays. Colostrum was used in one article to search for antibodies. In meta-analysis, PCR in AF showed the best performance for CT diagnosis based on the highest summary sensitivity (85.1%) and specificity (99.7%) added to lower magnitude heterogeneity. <i>Conclusion</i>. Most of the assays being researched to diagnose CT are basically the same traditional approaches available for clinical purposes. The range in diagnostic performance and the challenges imposed by CT diagnosis indicate the need to better explore pregnancy samples in search of new possibilities for diagnostic tools. Exploring immunological markers and using bioinformatics tools and <i>T. gondii</i> recombinant antigens should address the research needed for a new generation of diagnostic tools to face these challenges.","PeriodicalId":17527,"journal":{"name":"Journal of Tropical Medicine","volume":"1 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139922867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Unraveling Dengue Dynamics: In-Depth Epidemiological and Entomological Analyses in Bengaluru, India 揭示登革热动态:对印度班加罗尔的流行病学和昆虫学进行深入分析
IF 2.2 4区 医学 Q3 PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH Pub Date : 2024-02-10 DOI: 10.1155/2024/7247263
Vani H. C., Sujit Nath N., Jaiswal M. K., Tiwari R. P., Bharathi P., Asmita B., Shankar G., Jithen C., Pallavi V. D., Srinivas V., Ashok M., Mahamood Shariff, Renuka S., Shrinivasa B. M.
In view of the increased number of detected dengue cases in Bengaluru, a request for situation analysis was received from local health authorities in the selected area. The study included epidemiological and entomological assessments to understand the same. The immature forms collected were allowed to emerge, pooled, and processed for vector incrimination. In the surveyed population (347), 20 (5.8%) reported fever cases and 12 (3.5%) were confirmed as dengue cases among the 102 families. Stegomyia indices were high. Vector incrimination studies revealed pools positive for the presence of dengue virus in flower pots, fridge trays, plastic barrels, and rubber tires habitats. This study highlights the increased risk of dengue fever incidence in communities wherepoor intra and peri-domestic sanitation practices are prevailing and recommendsregular entomological surveillance of denguevirus in its vector population..
鉴于班加罗尔发现的登革热病例数量增加,选定地区的地方卫生当局要求进行情况分析。研究包括流行病学和昆虫学评估,以了解相同的情况。收集到的未成熟虫体被允许出现、汇集和处理,以便对病媒进行分类。在被调查的人口(347 人)中,有 20 个(5.8%)报告了发烧病例,在 102 个家庭中,有 12 个(3.5%)被确诊为登革热病例。登革热指数较高。病媒分类研究显示,花盆、冰箱托盘、塑料桶和橡胶轮胎中的登革热病毒呈阳性。这项研究强调,在家庭内部和周边卫生条件差的社区,登革热发病的风险会增加,因此建议定期对登革热病媒进行昆虫学监测。
{"title":"Unraveling Dengue Dynamics: In-Depth Epidemiological and Entomological Analyses in Bengaluru, India","authors":"Vani H. C., Sujit Nath N., Jaiswal M. K., Tiwari R. P., Bharathi P., Asmita B., Shankar G., Jithen C., Pallavi V. D., Srinivas V., Ashok M., Mahamood Shariff, Renuka S., Shrinivasa B. M.","doi":"10.1155/2024/7247263","DOIUrl":"https://doi.org/10.1155/2024/7247263","url":null,"abstract":"In view of the increased number of detected dengue cases in Bengaluru, a request for situation analysis was received from local health authorities in the selected area. The study included epidemiological and entomological assessments to understand the same. The immature forms collected were allowed to emerge, pooled, and processed for vector incrimination. In the surveyed population (347), 20 (5.8%) reported fever cases and 12 (3.5%) were confirmed as dengue cases among the 102 families. Stegomyia indices were high. Vector incrimination studies revealed pools positive for the presence of dengue virus in flower pots, fridge trays, plastic barrels, and rubber tires habitats. This study highlights the increased risk of dengue fever incidence in communities wherepoor intra and peri-domestic sanitation practices are prevailing and recommendsregular entomological surveillance of denguevirus in its vector population..","PeriodicalId":17527,"journal":{"name":"Journal of Tropical Medicine","volume":"43 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139771150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Breeding Habitat Preference of the Dengue Vector Mosquitoes Aedes aegypti and Aedes albopictus from Urban, Semiurban, and Rural Areas in Kurunegala District, Sri Lanka 斯里兰卡库鲁内加拉地区城市、半城市和农村地区登革热病媒蚊子埃及伊蚊和白纹伊蚊的繁殖生境偏好
IF 2.2 4区 医学 Q3 PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH Pub Date : 2024-01-29 DOI: 10.1155/2024/4123543
J. M. Manel K. Herath, W. A. Priyanka P. De Silva, Thilini C. Weeraratne, S. H. P. Parakrama Karunaratne
Elimination of vector mosquito larvae and their breeding environments is an effective strategy in dengue disease control. Present study examined larval density and water quality in breeding habitats and container preference of dengue vectors Ae. aegypti and Ae. albopictus. Larval surveys were conducted monthly in urban, semiurban, and rural sites in Kurunegala, Sri Lanka, from January 2019 to December 2021. Larval densities were recorded under the following three categories: type of container (16 types), type of material (6 types), and location (indoor/outdoor). Breeding preference ratios (BPRs) were calculated using Index of Available Containers and the Index of Contribution to Breeding Sites. Out of 19,234 wet containers examined, larval stages were found in 1,043 habitats. Ae. albopictus larvae were in all three areas whereas Ae. aegypti larvae were restricted to urban areas. Highest number of wet containers and highest positivity were reported from urban followed by semiurban. In general, discarded nondegradable items were the most frequent and mostly positive breeding sites. For Ae. aegypti, the most preferred breeding sites were gutters and concrete slabs. Ae. albopictus mostly preferred concrete slabs in urban areas and tyres in semiurban and rural areas. Material types such as rubber and concrete were mostly preferred by Ae. aegypti whereas ceramic was preferred by Ae. albopictus. Although plastic was the most available material type in all study sites, preference to plastic was low except for urban Ae. albopictus. Both species preferred urban indoor breeding habitats although outdoor breeding was preferred by Ae. albopictus in rural areas. Larval densities of Ae. aegypti and semiurban Ae. albopictus significantly correlated with the BPR of the container type and material type. Dengue vector larvae were found in a 6.7–9.4 pH range. Total dissolved solids and alkalinity positively correlated with preference. Information generated can be successfully used in waste management and public education for effective vector control.
消灭病媒蚊幼虫及其繁殖环境是控制登革热病的有效策略。本研究调查了登革热病媒埃及伊蚊和白纹伊蚊的幼虫密度、繁殖栖息地的水质以及对容器的偏好。从2019年1月至2021年12月,每月在斯里兰卡库鲁内加拉的城市、半城市和农村地区进行幼虫调查。幼虫密度记录分为以下三类:容器类型(16 种)、材料类型(6 种)和地点(室内/室外)。利用可用容器指数和繁殖地贡献指数计算繁殖偏好比率(BPRs)。在检查的 19,234 个湿容器中,在 1,043 个生境中发现了幼虫阶段。白纹伊蚊幼虫出现在所有三个地区,而埃及伊蚊幼虫则局限于城市地区。城市地区的湿容器数量最多,阳性率最高,其次是半城市地区。一般来说,不可降解的废弃物品是最常见的阳性繁殖地。埃及蚁最喜欢的繁殖地是排水沟和水泥板。白纹伊蚊在城市地区最喜欢水泥板,在半城市和农村地区则喜欢轮胎。埃及蚁主要喜欢橡胶和混凝土等材料类型,而白纹伊蚊则喜欢陶瓷。虽然塑料是所有研究地点中最容易获得的材料类型,但除了城市白纹伊蚊外,其他物种对塑料的偏好度都很低。两种白纹伊蚊都喜欢在城市室内繁殖,但农村地区的白纹伊蚊更喜欢在室外繁殖。埃及登革热蚁和半城市白纹伊蚊的幼虫密度与容器类型和材料类型的 BPR 显著相关。登革热病媒幼虫的 pH 值范围为 6.7-9.4。总溶解固体和碱度与偏好呈正相关。所生成的信息可成功用于废物管理和公众教育,以有效控制病媒。
{"title":"Breeding Habitat Preference of the Dengue Vector Mosquitoes Aedes aegypti and Aedes albopictus from Urban, Semiurban, and Rural Areas in Kurunegala District, Sri Lanka","authors":"J. M. Manel K. Herath, W. A. Priyanka P. De Silva, Thilini C. Weeraratne, S. H. P. Parakrama Karunaratne","doi":"10.1155/2024/4123543","DOIUrl":"https://doi.org/10.1155/2024/4123543","url":null,"abstract":"Elimination of vector mosquito larvae and their breeding environments is an effective strategy in dengue disease control. Present study examined larval density and water quality in breeding habitats and container preference of dengue vectors <i>Ae. aegypti</i> and <i>Ae. albopictus</i>. Larval surveys were conducted monthly in urban, semiurban, and rural sites in Kurunegala, Sri Lanka, from January 2019 to December 2021. Larval densities were recorded under the following three categories: type of container (16 types), type of material (6 types), and location (indoor/outdoor). Breeding preference ratios (BPRs) were calculated using Index of Available Containers and the Index of Contribution to Breeding Sites. Out of 19,234 wet containers examined, larval stages were found in 1,043 habitats. <i>Ae. albopictus</i> larvae were in all three areas whereas <i>Ae. aegypti</i> larvae were restricted to urban areas. Highest number of wet containers and highest positivity were reported from urban followed by semiurban. In general, discarded nondegradable items were the most frequent and mostly positive breeding sites. For <i>Ae</i>. <i>aegypti</i>, the most preferred breeding sites were gutters and concrete slabs. <i>Ae. albopictus</i> mostly preferred concrete slabs in urban areas and tyres in semiurban and rural areas. Material types such as rubber and concrete were mostly preferred by <i>Ae. aegypti</i> whereas ceramic was preferred by <i>Ae. albopictus</i>. Although plastic was the most available material type in all study sites, preference to plastic was low except for urban <i>Ae. albopictus</i>. Both species preferred urban indoor breeding habitats although outdoor breeding was preferred by <i>Ae. albopictus</i> in rural areas. Larval densities of <i>Ae. aegypti</i> and semiurban <i>Ae. albopictus</i> significantly correlated with the BPR of the container type and material type. Dengue vector larvae were found in a 6.7–9.4 pH range. Total dissolved solids and alkalinity positively correlated with preference. Information generated can be successfully used in waste management and public education for effective vector control.","PeriodicalId":17527,"journal":{"name":"Journal of Tropical Medicine","volume":"159 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139580761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
Journal of Tropical Medicine
全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1