The advancement of technology has revolutionized education, particularly through video-based learning. In response, the Good Practices in Animal Research (BPEA, "Boas Práticas em Experimentação Animal" in Portuguese) platform was established as a contemporary educational tool for training in laboratory rodent experimental techniques. Designed to replace traditional animal-centered teaching methods, BPEA provided scientifically accurate video content tailored for veterinary medicine students. Mastering animal handling skills is crucial for veterinary students, and BPEA addressed this by offering video demonstrations of experimental procedures, allowing visualization and confidence-building before live animal interaction. The platform's video library covered diverse procedures, such as substance administration and blood collection, accompanied by protocols, images, and diagrams for enhanced learning. The intuitive menu facilitated easy navigation, enabling students to access content aligned with their needs. Website traffic analysis demonstrated widespread usage, with users from Portuguese-speaking countries being prominent. Integration of BPEA into the Laboratory Animal Science course at the University of São Paulo garnered positive student feedback, highlighting its value as a supplementary resource for bridging theoretical and practical learning. While BPEA showed promise in promoting ethical teaching practices and reducing animal stress, it could not entirely replace hands-on training. A balanced approach between video-based learning and live demonstrations is necessary for a comprehensive learning experience. In conclusion, BPEA was a valuable resource contributing to Laboratory Animal Science education, aligning with ethical standards, and benefiting students, researchers, and animal care professionals. Continuous improvements based on feedback make the platform a dynamic tool for future advancements in laboratory animal science education.