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Mechanism of oxidative damage in Escherichia coli caused by epigallocatechin gallate (EGCG) in the presence of calcium ions 表没食子儿茶素没食子酸酯(EGCG)在钙离子存在下引起大肠杆菌氧化损伤的机制
Pub Date : 2023-03-10 DOI: 10.2166/aqua.2023.190
Cuimin Feng, Jiaohua Luo, Xuan-qi Liu, Lu-meng Huang, Hongri Yu, Chang-zheng Wang, Jin-shuang Zhang
Tea polyphenols can be developed into new types of disinfectants for drinking water. The antibacterial effect of epigallocatechin gallate (EGCG) on Escherichia coli (E. coli) in the presence of Ca2+ is affected by the Ca2+ concentration. The oxidative damage mechanism and oxidative damage process of EGCG in E. coli under the presence of Ca2+ were deeply analyzed under three aspects: reactive oxygen species (ROS), antioxidant system, and oxidative stress response in E. coli to provide a theoretical basis for the use of EGCG as a disinfectant in drinking water disinfection. EGCG leads to excessive production of superoxide anion in E. coli and the presence of Ca2+ promotes further imbalance of superoxide anion in E. coli; Ca2+ has little effect on EGCG hindering the scavenging of hydroxyl radicals in bacteria; EGCG can hinder the effect of antioxidant enzymes in E. coli, and Ca2+ has a particular regulatory effect on antioxidant enzymes, thus hindering the oxidative damage of EGCG to E. coli; Ca2+ can cause the expression of the oxyR and DPS genes, protect bacterial DNA, and prevent EGCG from damaging bacterial DNA. In the presence of a high concentration of Ca2+, it may activate the cell efflux pump through the soxS gene, resulting in E. coli resistance to EGCG.
茶多酚可开发为新型饮用水消毒剂。表没食子儿茶素没食子酸酯(EGCG)在Ca2+存在下对大肠杆菌(E. coli)的抑菌作用受Ca2+浓度的影响。从活性氧(ROS)、抗氧化系统、氧化应激反应三个方面深入分析了Ca2+存在下EGCG在大肠杆菌中的氧化损伤机制和氧化损伤过程,为EGCG作为消毒剂用于饮用水消毒提供理论依据。EGCG导致大肠杆菌超氧阴离子过量产生,Ca2+的存在促进大肠杆菌超氧阴离子进一步失衡;Ca2+对EGCG抑制细菌清除羟基自由基的作用不大;EGCG可以阻碍大肠杆菌中抗氧化酶的作用,而Ca2+对抗氧化酶具有特殊的调节作用,从而阻碍了EGCG对大肠杆菌的氧化损伤;Ca2+可以引起oxyR和DPS基因的表达,保护细菌DNA,防止EGCG破坏细菌DNA。在高浓度Ca2+存在的情况下,可能通过soxS基因激活细胞外排泵,导致大肠杆菌对EGCG产生耐药性。
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引用次数: 0
Assessment of water demand and potential water sources to face future water scarcity of hilly regions 针对未来丘陵地区水资源短缺的水需求和潜在水源评估
Pub Date : 2023-03-07 DOI: 10.2166/aqua.2023.212
K. V., P. R. Priya, Tharkeshwari K., Bineta Mazumder, P. Gayatri, Jackline Neha
In this work, an attempt is made to predict the future water demand of a hilly region. The study area is selected based on the population and the water demand. For this work, Namunaghar and Wandoor Panchayats of Andaman and Nicobar Islands are selected. Initially, preliminary studies such as the current population and future growth and their water demand were analyzed. Also, using a geographical information system (GIS), the study area is located to identify the surface and groundwater sources. From the GIS data, a detailed map is prepared to conduct the physical verification and collect the water quality measurement. From the population forecast, it was identified that 435,000 and 480,300 l of water are required to meet the water demand in the year 2031 Namunaghar and Wander Panchayat, respectively. It also assessed the GIS data and found out potential water sources of 157 (67 wells, 58 ponds, and other minor sources) and 485 (215 wells, 229 ponds, and other minor sources) at Namunaghar and Wandoor Panchayats, respectively. Finally, all the potential water sources were checked for quality for drinking purposes.
在这项工作中,试图预测一个丘陵地区未来的水需求。研究区域是根据人口和需水量选择的。为了这项工作,安达曼和尼科巴群岛的Namunaghar和Wandoor Panchayats被选中。首先,初步研究,如目前的人口和未来的增长和他们的水需求进行了分析。此外,利用地理信息系统(GIS)对研究区域进行定位,以确定地表水和地下水的来源。根据GIS数据,制作详细的地图进行物理验证并收集水质测量数据。根据人口预测,在2031年,Namunaghar和Wander Panchayat分别需要435,000和480,300升水来满足水需求。它还评估了GIS数据,分别在Namunaghar和Wandoor Panchayats发现了157个(67口井、58个池塘和其他小水源)和485个(215口井、229个池塘和其他小水源)的潜在水源。最后,对所有可能的饮用水源进行了水质检查。
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引用次数: 0
Mechanistic action of pesticides on pests and their consequent effect on fishes and human health with remediation strategies 农药对害虫的作用机理及其对鱼类和人类健康的影响及补救策略
Pub Date : 2023-03-07 DOI: 10.2166/aqua.2023.233
Anita Singh, Monika Mahajan, R. Kothari, Navinderdeep Singh, R. Singh
Population detonation and inflated demand for agricultural products have resulted in the rampant use of pesticides in recent years. These pesticides are used to reduce the number of pesticides by different mechanisms. They have been utilized in agriculture to expand agrarian profit, crop yield, quality, and storage life. The incessant and extensive use of resistant pesticides has contaminated the water bodies, fields, crops, and aquatic biota as well as poses a threat to human health. As a result, stringent regulations and limits are established to monitor the pesticide matrix. The current review focuses on pesticide contamination in the food chain, particularly from the aquatic bodies to fishes and humans. It also discusses strict regulations and limits including maximum residual limits for food items, acceptable daily intake, theoretical maximum daily intake, and estimated carcinogenicity/non-carcinogenicity for fishes and human health risks. In addition to conferring the negative effects of pesticides, this article discusses cost-effective remediation techniques such as phytoremediation, adsorption, the Fenton oxidation method, microalgal/high-rate algal ponds, and nanotechnology with the comparison of their remediation cost.
近年来,人口激增和对农产品需求的膨胀导致了农药的猖獗使用。这些农药通过不同的机制来减少农药的数量。它们已被用于农业,以扩大农业利润、作物产量、质量和储存寿命。抗药性农药的持续和广泛使用已经污染了水体、农田、作物和水生生物群,并对人类健康构成威胁。因此,制定了严格的法规和限制来监测农药基质。目前的审查侧重于食物链中的农药污染,特别是从水生生物到鱼类和人类的农药污染。它还讨论了严格的法规和限制,包括食品的最大残留限量、可接受的每日摄入量、理论最大每日摄入量,以及鱼类的估计致癌性/非致癌性和人类健康风险。除了农药的负面影响外,本文还讨论了具有成本效益的修复技术,如植物修复、吸附、Fenton氧化法、微藻/高速率藻池和纳米技术,并对其修复成本进行了比较。
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引用次数: 2
Development and optimization of the dye removal process by Trichoderma reesei using starch effluent as a growth supplement 里氏木霉以淀粉废水为生长补充物脱色工艺的开发与优化
Pub Date : 2023-03-01 DOI: 10.2166/aqua.2023.003
Shweta Kalia, Saurabh Samuchiwal, Vidushi Dhaka, A. Malik
The textile industry generates enormous starch effluent from the desizing process that can be utilized as a nutrient source for fungal growth and simultaneous dye decolorization. In the present study, Trichoderma reesei was used as a potential fungal isolate for the decolorization of reactive dyes using a minimal salt media for growth. The dye removal of Reactive blue 13, Reactive red 198, Reactive yellow 176, and Reactive black 5 were 95.35, 88.17, 86.01, and 94.84 mg L−1, respectively, by fungal biomass at 100 mg L−1 of initial dye concentration in 48 h was achieved. T. reesei showed decolorization of dyes at initial concentrations upto 500 mg L−1 with high dye uptake capacity. The glucose (5 g L−1) and yeast extracts (2.5 g L−1) were optimal for maximum dye decolorization. The utilization of starch effluent as an alternative nutrient source supplemented with 3.5 g L−1 glucose as growth media by T. reesei showed >85% of decolorization of Reactive blue 13 (100–200 mg L−1). Thus, starch effluent could be partially supplemented with glucose to support fungal growth and dye decolorization, eliminating the requirement of minimal salts for dye decolorization that follows a sustainable approach.
纺织工业在退浆过程中产生大量的淀粉废水,这些废水可以用作真菌生长的营养源,同时也可以用于染料脱色。在本研究中,里氏木霉被用作一种潜在的分离真菌,用于活性染料的脱色,使用最低盐培养基进行生长。在初始染料浓度为100 mg L−1的条件下,真菌生物量对活性蓝13、活性红198、活性黄176和活性黑5的去除率分别为95.35、88.17、86.01和94.84 mg L−1。T. reesei显示,在初始浓度高达500 mg L−1时,染料脱色能力强,染料吸收能力强。葡萄糖(5 g L−1)和酵母提取物(2.5 g L−1)对染料脱色效果最佳。利用淀粉废水作为替代营养源,并添加3.5 g L−1葡萄糖作为生长培养基,T. reesei对活性蓝13 (100-200 mg L−1)的脱色率>85%。因此,淀粉废水可以部分补充葡萄糖,以支持真菌生长和染料脱色,消除了对染料脱色的最低盐的要求,这是一种可持续的方法。
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引用次数: 0
A data quality assessment framework for drinking water distribution system water quality time series datasets 饮用水分配系统水质时间序列数据集的数据质量评估框架
Pub Date : 2023-03-01 DOI: 10.2166/aqua.2023.228
Killian Gleeson, S. Husband, John W. Gaffney, J. Boxall
The derivation of information from monitoring drinking water quality at high spatiotemporal resolution as it passes through complex, ageing distribution systems is limited by the variable data quality from the sensitive scientific instruments necessary. A framework is developed to overcome this. Application to three extensive real-world datasets, consisting of 92 multi-parameter water quality time series of data taken from different hardware configurations, shows how the algorithms can provide quality-assured data and actionable insight. Focussing on turbidity and chlorine, the framework consists of three steps to bridge the gap between data and information; firstly, an automated rule-based data quality assessment is developed and applied to each water quality sensor, then, cross-correlation is used to determine spatiotemporal relationships and finally, spatiotemporal information enables multi-sensor data quality validation. The framework provides a method to achieve automated data quality assurance, applicable to both historic and online datasets, such that insight and actionable insight can be gained to help ensure the supply of safe, clean drinking water to protect public health.
当饮用水质量通过复杂的、老化的分布系统时,以高时空分辨率监测饮用水质量的信息推导受到必要的敏感科学仪器的可变数据质量的限制。开发了一个框架来克服这个问题。应用于三个广泛的真实数据集,包括92个多参数水质时间序列数据,这些数据来自不同的硬件配置,显示了算法如何提供有质量保证的数据和可操作的见解。该框架以浊度和氯为重点,包括三个步骤,以弥合数据和信息之间的差距;首先,开发了基于规则的数据质量自动评估方法,并将其应用于每个水质传感器,然后利用相互关系确定时空关系,最后利用时空信息实现多传感器数据质量验证。该框架提供了一种实现自动化数据质量保证的方法,既适用于历史数据集,也适用于在线数据集,从而能够获得见解和可操作的见解,帮助确保提供安全、清洁的饮用水,以保护公众健康。
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引用次数: 0
GTAR: a new ensemble evolutionary autoregressive approach to model dissolved organic carbon GTAR:一种新的集成进化自回归方法来模拟溶解有机碳
Pub Date : 2023-03-01 DOI: 10.2166/aqua.2023.235
A. Danandeh Mehr, H. Marttila, Ali Torabi Haghighi, Danny Croghan, Nasrin Fathollahzadeh Attar
This article explores the forecasting capabilities of three classic linear and nonlinear autoregressive modeling techniques and proposes a new ensemble evolutionary time series approach to model and forecast daily dynamics in stream dissolved organic carbon (DOC). The model used data from the Oulankajoki River basin, a boreal catchment in Northern Finland. The models that were evolved used both accuracy and parsimony measures including autoregressive (AR), vector autoregressive (VAR), and self-exciting threshold autoregressive (SETAR). The new method, called genetic-based SETAR (GTAR), evolved through the integration of state-of-the-art genetic programming with SETAR. To develop the models, high-resolution DOC concentration and daily streamflow (as the external input for VAR) were measured at the same gauging station throughout the ice free season. The results showed that all the models characterize the DOC dynamics with an acceptable 1-day-ahead forecasting accuracy. Use of the streamflow time series as an exogenous variable did not increase the predictive accuracy of AR models. Moreover, the hybrid GTAR provided the best accuracy for the holdout testing data and proved to be a suitable approach for predicting DOC in boreal conditions.
本文探讨了三种经典线性和非线性自回归建模技术的预测能力,并提出了一种新的集成进化时间序列方法来模拟和预测河流溶解有机碳(DOC)的日动态。该模型使用了芬兰北部北部集水区奥兰卡约基河流域的数据。进化的模型使用了准确性和简约性措施,包括自回归(AR)、向量自回归(VAR)和自激阈值自回归(SETAR)。这种新方法被称为基于遗传的SETAR (GTAR),是通过将最先进的遗传规划与SETAR相结合而发展起来的。为了开发模型,在整个无冰季节在同一测量站测量了高分辨率DOC浓度和日流量(作为VAR的外部输入)。结果表明,所有模型均能较好地描述DOC动态,预报精度可接受。使用流时间序列作为外生变量并没有增加AR模型的预测精度。此外,混合GTAR为固结试验数据提供了最好的精度,并被证明是北方条件下预测DOC的合适方法。
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引用次数: 1
Statistical evaluation of snow accumulation and depletion from remotely sensed MODIS snow time series data using the SARIMA model 基于SARIMA模型的遥感MODIS积雪时间序列积雪积累和耗竭的统计评价
Pub Date : 2023-02-22 DOI: 10.2166/aqua.2023.231
Mohit Kumar, R. K. Tiwari, K. Kumar, K. S. Rautela
In the remote and challenging terrain of the Himalayan region, accurate measurement of cyclic snow accumulation and depletion is a significant challenge. To overcome this, an attempt has been made in the present study by applying a statistical analysis of MODIS snow time series data with the Seasonal Autoregressive Integrated Moving Average (SARIMA) model from 2003 to 2018 over the Beas river basin. The Box–Jenkins methodology of forecasting is based on the identification using seasonality, stationarity, ACF, and PACF plots; and estimation based on maximum likelihood techniques; and the last diagnostic checking based on the residual and error values have been used. Later, forecasting models have been proposed separately for the snow accumulation period (October–February) as (1,1,1) (0,1,3)19 and for the snow depletion period (March–September) as (1,1,1) (1,1,2)27 after calibration of the data (2003–2015) and the same were then validated using data (2016–2018). The accuracy assessment of the models has been checked using performance criteria like AIC, MSE, and RSS. The comparison of the forecasting models with the observed data showed a good agreement with R2 of 0.83 and 0.89 for snow accumulation and snow depletion, respectively. This research highlights the potential of utilizing satellite data and statistical modeling to address the challenges of monitoring snow cover in remote and inaccessible regions.
在喜马拉雅地区的偏远和具有挑战性的地形,准确测量循环积雪和损耗是一个重大的挑战。为了克服这一问题,本研究利用2003 - 2018年季节自回归综合移动平均(SARIMA)模型对双鱼河流域MODIS积雪时间序列数据进行了统计分析。Box-Jenkins预测方法基于季节性、平稳性、ACF和PACF图的识别;以及基于极大似然技术的估计;最后利用残差值和误差值进行诊断检查。随后,在对2003-2015年数据进行校正后,分别提出了积雪期(10 - 2月)的预测模型为(1,1,1)(0,1,3)19和积雪枯竭期(3 - 9月)的预测模型为(1,1,1)(1,1,2)27,并利用2016-2018年数据进行了验证。使用AIC、MSE和RSS等性能标准检查了模型的准确性评估。预报模式与实测资料的对比表明,积雪和雪损的R2分别为0.83和0.89,符合较好。这项研究强调了利用卫星数据和统计建模来解决监测偏远和难以到达地区积雪的挑战的潜力。
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引用次数: 0
Improvement of water utility management in Tajikistan: reduction in water wastage using a metered tariff system 改善塔吉克斯坦的水务管理:使用计量收费制度减少水的浪费
Pub Date : 2023-02-21 DOI: 10.2166/aqua.2023.078
Ryuji Ogata, Kazumi Matsuda, Tabarzoda Jamshed Avzal, Kimio Abe
Tajikistan is a country in Central Asia with rich water resources. However, drinking water is used for agriculture and other non-drinking purposes owing to the fixed tariff system. Consequently, the overall level of water services has deteriorated and generated revenue is insufficient for operation and maintenance costs. Therefore, this study aimed to improve the quality of water supply services by providing hardware support, such as the construction of wells and the renewal of water distribution pipes. However, because hardware improvement alone cannot sufficiently enhance water supply services, software support to improve the capacity of the Vodokanal (utility) staff, including the preparation of a metered tariff system, was implemented through a technical cooperation project. The project successfully raised customer awareness of water conservation and the water consumption rate was reduced to two-thirds of that before the implementation of the project. Additionally, the water quantity, quality, pressure, and supply hours in the targeted area were significantly improved. Despite the resulting increase in financial burden, customer satisfaction regarding the water supply was enhanced from 51% before the project to 100% in the second half of the project. Simultaneously, the water supply revenue increased by 24% and this increase contributed to sustainable water supply management.
塔吉克斯坦是中亚国家,水资源丰富。但是,由于固定的关税制度,饮用水被用于农业和其他非饮用目的。因此,供水服务的总体水平已经恶化,产生的收入不足以支付业务和维修费用。因此,本研究旨在通过提供硬件支持来提高供水服务质量,例如建造井和更新配水管。但是,由于单靠硬件改进不能充分提高供水服务,因此通过一个技术合作项目执行了软件支助,以提高Vodokanal(公用事业)工作人员的能力,包括编制一个计量收费制度。项目成功地提高了客户节约用水的意识,用水量下降到项目实施前的三分之二。此外,目标区域的水量、水质、压力和供水时间均有显著改善。尽管因此增加了财政负担,但客户对供水的满意度从项目前的51%提高到项目后半段的100%。同时,供水收入增加了24%,这一增长有助于可持续供水管理。
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引用次数: 0
Efficacy testing of non-oxidizing biocides for polyamide membrane biofouling prevention using a modified CDC biofilm reactor 改良CDC生物膜反应器非氧化性杀菌剂对聚酰胺膜生物污染防治效果的试验研究
Pub Date : 2023-02-20 DOI: 10.2166/aqua.2023.217
Luiz H. Da-Silva-Correa, Kirsten Aasen, Nicole E. Gamm, Rafaela Godoy, Negar Rahmati, H. Buckley
Biofouling is one of the most challenging obstacles faced by reverse osmosis (RO) membrane systems to supply potable water. Currently, biofouling is imperfectly handled by RO feed water pre-chlorination, which is associated with the production of carcinogenic disinfection by-products. To propose a safer alternative to control biofouling in RO drinking water applications, this study investigates the efficacy of five biocides to prevent and remove Pseudomonas aeruginosa biofilms from RO membranes: (1) 2-methyl-4-isothiazolin-3-one (MIT); (2) 2,2-dibromo-3-nitrilopropionamide (DBNPA); (3) sodium bisulfite (SBS); (4) sodium benzoate (SB), and (5) ethyl lauroyl arginate (LAE). Experiments were conducted on the Center for Disease Control (CDC) Biofilm Reactor (CBR) with biocidal dosing estimated on 96-well microtiter plates. Confocal Scanning Laser Microscopy (CLSM) and Scanning Electron Microscopy (SEM) were used to analyze the biocides' anti-biofilm efficacies under dynamic conditions relative to minimum biofilm inhibitory and eradication concentrations. The results in this study indicated that LAE presented the best anti-biofilm efficacies in treating P. aeruginosa biofilms when compared to all studied biocides; it not only prevented biofilm formation (>98%) but also it effectively removed pre-established biofilms (>99%) from RO membrane coupons. Therefore, due to safety and efficacy, LAE is an excellent candidate for controlling biofouling in drinking water RO membrane systems.
生物污染是反渗透(RO)膜系统供应饮用水面临的最具挑战性的障碍之一。目前,反渗透给水预加氯处理生物污垢效果不理想,会产生致癌的消毒副产物。为了提出一种更安全的替代方法来控制RO饮用水应用中的生物污染,本研究研究了五种杀菌剂对RO膜上铜绿假单胞菌生物膜的预防和去除效果:(1)2-甲基-4-异噻唑啉-3-酮(MIT);(2) 2,2-二溴-3-硝基丙酰胺(DBNPA);亚硫酸氢钠(SBS);(4)苯甲酸钠(SB)和(5)月桂酰精酸乙酯(LAE)。在美国疾病控制与预防中心(CDC)生物膜反应器(CBR)上进行了实验,并在96孔微滴板上估计了生物杀灭剂量。采用共聚焦扫描激光显微镜(CLSM)和扫描电镜(SEM)分析了动态条件下杀菌剂相对于最低生物膜抑制和根除浓度的抗生物膜效果。本研究结果表明,与所有被研究的杀菌剂相比,LAE对铜绿假单胞菌生物膜的抗生物膜效果最好;它不仅阻止了生物膜的形成(>98%),而且有效地去除了RO膜上预先形成的生物膜(>99%)。因此,由于其安全性和有效性,LAE是控制饮用水反渗透膜系统中生物污染的理想候选者。
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引用次数: 0
A pilot study on the dual membrane process of ultrafiltration and nanofiltration to treat algae-laden raw water compared to O3-BAC 超滤/纳滤双膜工艺处理含藻原水与O3-BAC的中试研究
Pub Date : 2023-02-20 DOI: 10.2166/aqua.2023.191
Xingqi Ding, Zhenxun Yu, Huaqiang Chu, D. Wang, Kun Liu, Buyun Wang, Caihuan Tong, B. Dong
In this study, a dual membrane process (DMP) that combines seven-hole ultrafiltration and nanofiltration was designed and compared to O3-biological activated carbon (O3-BAC) for high-quality drinking water production. The pollutant removal, membrane fouling, long-term operational characteristics, and technical economy were systematically investigated using raw water from Tai Lake, which has a high algae content. The results elucidate that the DMP has superb decontamination. This method has much better removal of turbidity, CODMn, UV254, and algae than O3-BAC. Its removal of ammonia nitrogen and fluorescent substances is slightly lower than that of O3-BAC, but the effluent still satisfies the drinking water standard. The DMP is also much more capable of dealing with high algae-laden raw water. Compared to O3-BAC, the cost of the DMP is 46.4% higher per ton without consuming chemicals, so it is more environmentally friendly. In summary, the DMP offers a promising and effective technology to treat high algae-laden water with the advantages of high stability, reliable effluent, and zero emissions.
本研究设计了七孔超滤和纳滤相结合的双膜工艺(DMP),并与o3 -生物活性炭(O3-BAC)进行了比较。以藻类含量高的太湖原水为原料,系统地研究了其污染物去除、膜污染、长期运行特性和技术经济性。结果表明,DMP具有良好的去污性能。与O3-BAC相比,该方法对浊度、CODMn、UV254和藻类的去除效果更好。其对氨氮和荧光物质的去除率略低于O3-BAC,但出水仍满足饮用水标准。DMP也更有能力处理高藻类含量的原水。与O3-BAC相比,DMP在不消耗化学品的情况下,每吨成本高出46.4%,因此更环保。综上所述,DMP具有高稳定性、出水可靠、零排放等优点,是一种有前途的、有效的高藻类水处理技术。
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引用次数: 0
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Journal of Water Supply: Research and Technology-Aqua
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