E. Akade, A. Azaran, Bijan Kikhaei, Saeid Bitaraf, Shahram Jalilian
Background: Human Parvovirus 4 (P4) is a non-enveloped, single-stranded DNA virus belonging to the Tetraparvovirus genus within the Parvoviridae family. Epidemiologically, P4 exhibits similarities with its well-established family counterpart, primate erythroparvovirus 1 (known as B19), a blood-borne virus implicated in causing aplastic crises in individuals with sickle cell disease (SCD). Despite the acknowledged association with B19, there is a dearth of prior investigations into the prevalence and clinical significance of P4 in SCD patients. Objectives: This study aims to ascertain the prevalence and outcomes of P4, along with exploring the co-prevalence of P4 with B19 in individuals with SCD. Methods: A total of 162 participants were enrolled, comprising 120 individuals with SCD and 45 healthy controls. The prevalence of P4 and B19 DNA was determined utilizing a nested-PCR method. Sequencing was performed on positive samples to validate the diagnosis, and a phylogenetic tree was constructed based on the sequencing results. Correlations in the data were analyzed using statistical methods. Results: The prevalence of P4 and B19, as well as the co-prevalence of these viruses, was significantly higher in SCD patients than in healthy individuals. Moreover, the prevalence of P4 did not exhibit a significant correlation with variables such as age, sex, aplastic crises, or specific complications associated with SCD. Conclusions: Sickle cell disease patients represent a susceptible population for P4 infection, as indicated by the heightened prevalence observed in this study.
{"title":"Co-prevalence of Human Parvovirus 4 and Primate Erythroparvovirus 1 (B19) in Sickle Cell Disease and Healthy Populations","authors":"E. Akade, A. Azaran, Bijan Kikhaei, Saeid Bitaraf, Shahram Jalilian","doi":"10.5812/jjm-145003","DOIUrl":"https://doi.org/10.5812/jjm-145003","url":null,"abstract":"Background: Human Parvovirus 4 (P4) is a non-enveloped, single-stranded DNA virus belonging to the Tetraparvovirus genus within the Parvoviridae family. Epidemiologically, P4 exhibits similarities with its well-established family counterpart, primate erythroparvovirus 1 (known as B19), a blood-borne virus implicated in causing aplastic crises in individuals with sickle cell disease (SCD). Despite the acknowledged association with B19, there is a dearth of prior investigations into the prevalence and clinical significance of P4 in SCD patients. Objectives: This study aims to ascertain the prevalence and outcomes of P4, along with exploring the co-prevalence of P4 with B19 in individuals with SCD. Methods: A total of 162 participants were enrolled, comprising 120 individuals with SCD and 45 healthy controls. The prevalence of P4 and B19 DNA was determined utilizing a nested-PCR method. Sequencing was performed on positive samples to validate the diagnosis, and a phylogenetic tree was constructed based on the sequencing results. Correlations in the data were analyzed using statistical methods. Results: The prevalence of P4 and B19, as well as the co-prevalence of these viruses, was significantly higher in SCD patients than in healthy individuals. Moreover, the prevalence of P4 did not exhibit a significant correlation with variables such as age, sex, aplastic crises, or specific complications associated with SCD. Conclusions: Sickle cell disease patients represent a susceptible population for P4 infection, as indicated by the heightened prevalence observed in this study.","PeriodicalId":17803,"journal":{"name":"Jundishapur Journal of Microbiology","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2024-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141006984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Khadijeh Khanaliha, Tahereh Donyavi, S. Monavari, AliReza Khatami, Javid Sadri Nahand, S. J. Kiani, Ahmad Tavakoli, Mahdi Ramshyny, F. Bokharaei-Salim
Background: Persistent detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA in individuals who have recovered from coronavirus disease 2019 (COVID-19) remains an unexplained phenomenon warranting further study. Recent research suggests that this RNA could be the result of transcription from an integrated SARS-CoV-2 genome. Objectives: This study aimed to investigate the presence of the DNA form of the SARS-CoV-2 genome in oropharyngeal, nasopharyngeal, and peripheral blood mononuclear cell (PBMC) samples from COVID-19 patients with prolonged viral detection. Methods: We examined the presence of the reverse-transcribed viral genome in samples from eighty COVID-19 patients, including 40 outpatients (group 1), 40 hospitalized patients (group 2), and 40 healthy individuals (group 3), using a TaqMan® based real-time RT-PCR assay. Results: The mean ages of groups 1, 2, and 3 were 36.1 ± 11.0, 61.6 ± 18.4, and 39.0 ± 8.7, respectively. The molecular tests did not detect viral DNA forms, which may be produced during the SARS-CoV-2 life cycle, in the examined samples. Conclusions: Although no evidence of integrated viral DNA was found in this study, further research is essential to confirm these findings and explore the underlying mechanisms of prolonged SARS-CoV-2 RNA presence in recovered COVID-19 patients.
{"title":"Evaluation of Integrated SARS-CoV-2 Genome Presence in PBMC, Oropharyngeal, and Nasopharyngeal Samples of COVID-19 Patients","authors":"Khadijeh Khanaliha, Tahereh Donyavi, S. Monavari, AliReza Khatami, Javid Sadri Nahand, S. J. Kiani, Ahmad Tavakoli, Mahdi Ramshyny, F. Bokharaei-Salim","doi":"10.5812/jjm-145397","DOIUrl":"https://doi.org/10.5812/jjm-145397","url":null,"abstract":"Background: Persistent detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA in individuals who have recovered from coronavirus disease 2019 (COVID-19) remains an unexplained phenomenon warranting further study. Recent research suggests that this RNA could be the result of transcription from an integrated SARS-CoV-2 genome. Objectives: This study aimed to investigate the presence of the DNA form of the SARS-CoV-2 genome in oropharyngeal, nasopharyngeal, and peripheral blood mononuclear cell (PBMC) samples from COVID-19 patients with prolonged viral detection. Methods: We examined the presence of the reverse-transcribed viral genome in samples from eighty COVID-19 patients, including 40 outpatients (group 1), 40 hospitalized patients (group 2), and 40 healthy individuals (group 3), using a TaqMan® based real-time RT-PCR assay. Results: The mean ages of groups 1, 2, and 3 were 36.1 ± 11.0, 61.6 ± 18.4, and 39.0 ± 8.7, respectively. The molecular tests did not detect viral DNA forms, which may be produced during the SARS-CoV-2 life cycle, in the examined samples. Conclusions: Although no evidence of integrated viral DNA was found in this study, further research is essential to confirm these findings and explore the underlying mechanisms of prolonged SARS-CoV-2 RNA presence in recovered COVID-19 patients.","PeriodicalId":17803,"journal":{"name":"Jundishapur Journal of Microbiology","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2024-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140680237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: Colorectal cancer (CRC) is a major global health concern, and the link with Fusobacterium nucleatum has received considerable attention. Objectives: This study aimed to explore the prevalence of F. nucleatum and to assess the expression of the msh2, mlh1, and msh6 genes in CRC patients compared to a control group using real-time PCR. Methods: Forty CRC patients and twenty individuals from a control group participated in this study. Gastroenterologists collected biopsy specimens from which DNA and RNA were extracted using a specialized tissue extraction kit. Complementary DNA (cDNA) was then synthesized. Real-time PCR was employed to evaluate the expression levels of the msh2, mlh1, and msh6 genes and the presence of the F. nucleatum-specific 16srRNA gene to determine the relative prevalence of this bacterium in each group. Results: Results indicated a higher prevalence of the F. nucleatum-specific 16srRNA gene in CRC patients than in the control group. Additionally, expression levels of the msh2, mlh1, and msh6 genes were significantly higher in the cancer group, suggesting their role in CRC pathogenesis. The distribution of F. nucleatum was particularly high in the sigmoid and rectum areas of the colon. Conclusions: This study underscores the significance of F. nucleatum in CRC and provides insights into its association with altered gene expression patterns. Understanding the prevalence of F. nucleatum and its impact on msh2, mlh1, and msh6 genes may aid in developing improved diagnostic and therapeutic strategies for CRC. Further research is necessary to elucidate these relationships more comprehensively.
{"title":"Associations Between Fusobacterium nucleatum and msh2, mlh1, and msh6 Gene Expression in Colorectal Cancer","authors":"Reza Torkashvand, Bahareh Hajikhani, Reza Hosseini Doust, Hossein Dabiri, M. Dadashi","doi":"10.5812/jjm-144247","DOIUrl":"https://doi.org/10.5812/jjm-144247","url":null,"abstract":"Background: Colorectal cancer (CRC) is a major global health concern, and the link with Fusobacterium nucleatum has received considerable attention. Objectives: This study aimed to explore the prevalence of F. nucleatum and to assess the expression of the msh2, mlh1, and msh6 genes in CRC patients compared to a control group using real-time PCR. Methods: Forty CRC patients and twenty individuals from a control group participated in this study. Gastroenterologists collected biopsy specimens from which DNA and RNA were extracted using a specialized tissue extraction kit. Complementary DNA (cDNA) was then synthesized. Real-time PCR was employed to evaluate the expression levels of the msh2, mlh1, and msh6 genes and the presence of the F. nucleatum-specific 16srRNA gene to determine the relative prevalence of this bacterium in each group. Results: Results indicated a higher prevalence of the F. nucleatum-specific 16srRNA gene in CRC patients than in the control group. Additionally, expression levels of the msh2, mlh1, and msh6 genes were significantly higher in the cancer group, suggesting their role in CRC pathogenesis. The distribution of F. nucleatum was particularly high in the sigmoid and rectum areas of the colon. Conclusions: This study underscores the significance of F. nucleatum in CRC and provides insights into its association with altered gene expression patterns. Understanding the prevalence of F. nucleatum and its impact on msh2, mlh1, and msh6 genes may aid in developing improved diagnostic and therapeutic strategies for CRC. Further research is necessary to elucidate these relationships more comprehensively.","PeriodicalId":17803,"journal":{"name":"Jundishapur Journal of Microbiology","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2024-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140680903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
L. Shokoohizadeh, T. Dehghani, Vahideh Namordizadeh, A. Karmostaji
Background: Staphylococcus aureus is a significant bacterial pathogen globally recognized as the primary cause of numerous uncomplicated skin infections and severe invasive infections. The emergence of methicillin-resistant S. aureus (MRSA) poses a serious threat, leading to severe infections in both hospitals and community settings. Objectives: The aim of this study was to identify antibiotic resistance patterns and perform molecular classification of S. aureus strains isolated from both hospital and community settings in southern Iran. Methods: In this cross-sectional study conducted in Bandar Abbas between 2020 and 2021, a total of 156 clinical strains of S. aureus were collected. Antibiotic susceptibility was determined using the disk-diffusion agar method. The presence of the pvl gene, Sccmec types, and Agr group was identified through PCR analysis. Additionally, Multilocus sequence typing (MLST) was performed on selected isolates. Results: The study identified 156 strains, with 79 obtained from inpatients and 77 from outpatients, sourced from clinical samples. Among these isolates, 70 (44.8%) were classified as MRSA. The highest resistance was noted against azithromycin (83%), while the lowest resistance was observed for linezolid (5%) and gentamicin (7%). The presence of the pvl gene was detected in isolates from both hospital and community sources. Significant differences were noted in the occurrence of agr I and agr III genes between hospital and community isolates. Sccmec III was more predominant than other SCCmec types. Furthermore, MLST analysis revealed the presence of five distinct novel sequence types (STs): ST8634, ST8640, ST8650, ST8152, and ST8153. Conclusions: The findings indicate that the potential spread of hospital-acquired S. aureus strains to the community and vice versa poses a significant public health risk. This underscores the urgent need for robust infection control strategies and the identification of potential environmental and hospital sources of resistant strains, particularly MRSA strains.
背景：金黄色葡萄球菌是一种重要的细菌病原体，全球公认它是许多无并发症皮肤感染和严重侵入性感染的主要病因。耐甲氧西林金黄色葡萄球菌（MRSA）的出现构成了严重威胁，可导致医院和社区环境中的严重感染。研究目的本研究旨在确定抗生素耐药性模式，并对从伊朗南部医院和社区环境中分离出的金黄色葡萄球菌菌株进行分子分类。研究方法这项横断面研究于 2020 年至 2021 年在 Bandar Abbas 进行，共收集了 156 株金黄色葡萄球菌临床菌株。采用盘扩散琼脂法测定抗生素敏感性。通过 PCR 分析确定了 pvl 基因、Sccmec 类型和 Agr 组的存在。此外，还对部分分离物进行了多焦点序列分型（MLST）。研究结果研究发现了 156 株菌株，其中 79 株来自住院病人，77 株来自门诊病人，均来自临床样本。在这些分离株中，有 70 株（44.8%）被归类为 MRSA。耐药性最高的是阿奇霉素（83%），耐药性最低的是利奈唑胺（5%）和庆大霉素（7%）。在来自医院和社区的分离株中都检测到了 pvl 基因。医院和社区分离物中的 agr I 和 agr III 基因出现率存在显著差异。与其他 SCCmec 类型相比，Sccmec III 更占优势。此外，MLST 分析显示存在五种不同的新型序列类型（ST）：ST8634、ST8640、ST8650、ST8152 和 ST8153。结论研究结果表明，医院感染的金黄色葡萄球菌菌株有可能传播到社区，反之亦然，这对公共卫生构成了重大风险。这突出表明，迫切需要采取强有力的感染控制策略，并确定耐药菌株（尤其是 MRSA 菌株）的潜在环境和医院来源。
{"title":"New Sequence Types of Staphylococcus aureus Strains Isolated from Hospitals and Community Settings in Southern Iran","authors":"L. Shokoohizadeh, T. Dehghani, Vahideh Namordizadeh, A. Karmostaji","doi":"10.5812/jjm-144398","DOIUrl":"https://doi.org/10.5812/jjm-144398","url":null,"abstract":"Background: Staphylococcus aureus is a significant bacterial pathogen globally recognized as the primary cause of numerous uncomplicated skin infections and severe invasive infections. The emergence of methicillin-resistant S. aureus (MRSA) poses a serious threat, leading to severe infections in both hospitals and community settings. Objectives: The aim of this study was to identify antibiotic resistance patterns and perform molecular classification of S. aureus strains isolated from both hospital and community settings in southern Iran. Methods: In this cross-sectional study conducted in Bandar Abbas between 2020 and 2021, a total of 156 clinical strains of S. aureus were collected. Antibiotic susceptibility was determined using the disk-diffusion agar method. The presence of the pvl gene, Sccmec types, and Agr group was identified through PCR analysis. Additionally, Multilocus sequence typing (MLST) was performed on selected isolates. Results: The study identified 156 strains, with 79 obtained from inpatients and 77 from outpatients, sourced from clinical samples. Among these isolates, 70 (44.8%) were classified as MRSA. The highest resistance was noted against azithromycin (83%), while the lowest resistance was observed for linezolid (5%) and gentamicin (7%). The presence of the pvl gene was detected in isolates from both hospital and community sources. Significant differences were noted in the occurrence of agr I and agr III genes between hospital and community isolates. Sccmec III was more predominant than other SCCmec types. Furthermore, MLST analysis revealed the presence of five distinct novel sequence types (STs): ST8634, ST8640, ST8650, ST8152, and ST8153. Conclusions: The findings indicate that the potential spread of hospital-acquired S. aureus strains to the community and vice versa poses a significant public health risk. This underscores the urgent need for robust infection control strategies and the identification of potential environmental and hospital sources of resistant strains, particularly MRSA strains.","PeriodicalId":17803,"journal":{"name":"Jundishapur Journal of Microbiology","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2024-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140683580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Saina Karami, Reza Arjmand, J. Saki, Dian Dayer, A. Jelowdar
Background: Leishmania spp. protozoa cause leishmaniasis by infecting macrophages. Long non-coding RNAs (lncRNAs), such as H19, Metastasis Associated Lung Adenocarcinoma Transcript 1 (MALAT), HOX Antisense Intergenic RNA (HOTAIR), and TNF and HNRNPL Related Immuno-regulatory lncRNA (THRIL), play a role in macrophage polarization and gene regulation. Additionally, leukocytes can synthesize and respond to melatonin, yet the regulatory role of melatonin in Leishmania major-infected macrophages is not well understood. Objectives: This study aimed to assess the impact of melatonin on the expression of lncRNAs like H19, MALAT, HOTAIR, and THRIL, as well as on nitric oxide synthase (NOS) activity in L. major-infected macrophages. Methods: Leishmania major promastigotes and U937 cell lines were cultured. Macrophages were infected with the promastigotes and subsequently treated with melatonin at concentrations of 3, 10, 30, and 100 nM for durations of 4, 24, and 48 hours. The expression levels of the lncRNAs and NOS activity were measured using quantitative Polymerase Chain Reaction (q-PCR) and spectrophotometry, respectively. Results: Melatonin treatment (100 nM) significantly increased the expression of H19 compared to the control after 48 hours (P = 0.002). There was also a significant upregulation of MALAT and HOTAIR in macrophages treated with 3 nM melatonin compared to controls after 48 hours (P = 0.02 and P = 0.003, respectively). Additionally, THRIL expression significantly increased in the melatonin group (10 nM) compared to the control after 4 hours of treatment (P = 0.003). An increase in NOS activity was observed in the melatonin group (100 nM) compared to the control at 4 hours, 24 hours, and 48 hours (P = 0.034, P = 0.011, and P = 0.014, respectively). Conclusions: The findings suggest that melatonin may enhance the expression of H19, THRIL, MALAT, and HOTAIR, as well as NOS activity in macrophages infected with L. major. The upregulation of these lncRNAs by melatonin could potentially improve the macrophages' ability to combat L. major infection.
{"title":"The Effect of Melatonin on the Expression of H19, MALAT, HOTAIR, THRIL, and NOS Activity in Leishmania major-Infected Macrophages","authors":"Saina Karami, Reza Arjmand, J. Saki, Dian Dayer, A. Jelowdar","doi":"10.5812/jjm-143683","DOIUrl":"https://doi.org/10.5812/jjm-143683","url":null,"abstract":"Background: Leishmania spp. protozoa cause leishmaniasis by infecting macrophages. Long non-coding RNAs (lncRNAs), such as H19, Metastasis Associated Lung Adenocarcinoma Transcript 1 (MALAT), HOX Antisense Intergenic RNA (HOTAIR), and TNF and HNRNPL Related Immuno-regulatory lncRNA (THRIL), play a role in macrophage polarization and gene regulation. Additionally, leukocytes can synthesize and respond to melatonin, yet the regulatory role of melatonin in Leishmania major-infected macrophages is not well understood. Objectives: This study aimed to assess the impact of melatonin on the expression of lncRNAs like H19, MALAT, HOTAIR, and THRIL, as well as on nitric oxide synthase (NOS) activity in L. major-infected macrophages. Methods: Leishmania major promastigotes and U937 cell lines were cultured. Macrophages were infected with the promastigotes and subsequently treated with melatonin at concentrations of 3, 10, 30, and 100 nM for durations of 4, 24, and 48 hours. The expression levels of the lncRNAs and NOS activity were measured using quantitative Polymerase Chain Reaction (q-PCR) and spectrophotometry, respectively. Results: Melatonin treatment (100 nM) significantly increased the expression of H19 compared to the control after 48 hours (P = 0.002). There was also a significant upregulation of MALAT and HOTAIR in macrophages treated with 3 nM melatonin compared to controls after 48 hours (P = 0.02 and P = 0.003, respectively). Additionally, THRIL expression significantly increased in the melatonin group (10 nM) compared to the control after 4 hours of treatment (P = 0.003). An increase in NOS activity was observed in the melatonin group (100 nM) compared to the control at 4 hours, 24 hours, and 48 hours (P = 0.034, P = 0.011, and P = 0.014, respectively). Conclusions: The findings suggest that melatonin may enhance the expression of H19, THRIL, MALAT, and HOTAIR, as well as NOS activity in macrophages infected with L. major. The upregulation of these lncRNAs by melatonin could potentially improve the macrophages' ability to combat L. major infection.","PeriodicalId":17803,"journal":{"name":"Jundishapur Journal of Microbiology","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2024-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140693626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Patricia Vargas-Gutierrez, J. Silva-Sánchez, F. Uribe-Salas, Federico Lopez-Jasso, Evelyn Yveth Juarez-Perez, M. D. R. González-Martínez, Humberto Barrios Camacho
Background: The urgent need for antimicrobial research to address the escalating global challenge of β-lactam antibiotic resistance, particularly in Escherichia coli (E. coli)-induced urinary tract infections (UTI), is underscored by the increasing resistance to ciprofloxacin in Latin America. This issue has led to a heightened dependence on alternative therapeutics, such as cephalosporins. The identification of extended-spectrum β-lactamase (ESBL)-producing E. coli, notably the O25b-ST131 clone, adds complexity to UTI management. The prevalence of ESBL-producing E. coli varies globally due to factors including regional antimicrobial usage practices. Objectives: The goal of this study was to identify and molecularly characterize ESBL-producing E. coli isolates to identify the pandemic O25b-ST131 clone related to UTIs in one healthcare institution in Mexico. Methods: Bacterial species identification and antibiotic susceptibility tests were performed using the VITEK 2. The ESBL genes were identified using polymerase chain reaction (PCR). The E. coli genotyping was carried out by the phylogenetic group analysis and the O25b-ST131 identification. Results: A total of 86 unique E. coli isolates were confirmed as ESBL, and 75% were obtained from UTIs. The most prevalent β-lactamase genes were blaCTX-M (66%), blaTEM (8.1%), blaCTX-M/SHV (5.8%), blaCTX-M/TEM (4.6%), and blaSHV (2.3%). The B2 phylogroup was most prominent (54.4%), with 46.5% identified as a globally pandemic O25b-ST131 clone. No evident relationship was observed using random amplified polymorphic DNA (RAPD) between nosocomial and community-acquired infections in ESBL-producing E. coli isolates. Conclusions: The obtained findings highlight the significance of monitoring molecular epidemiology in antibiotic resistance profiles of the O25b-ST131 E. coli clone.
{"title":"Molecular Characterization of Extended Spectrum β-Lactamase -Producing Escherichia coli: Insights into the O25b-ST131 Clone in Mexican Urinary Tract Infections","authors":"Patricia Vargas-Gutierrez, J. Silva-Sánchez, F. Uribe-Salas, Federico Lopez-Jasso, Evelyn Yveth Juarez-Perez, M. D. R. González-Martínez, Humberto Barrios Camacho","doi":"10.5812/jjm-143352","DOIUrl":"https://doi.org/10.5812/jjm-143352","url":null,"abstract":"Background: The urgent need for antimicrobial research to address the escalating global challenge of β-lactam antibiotic resistance, particularly in Escherichia coli (E. coli)-induced urinary tract infections (UTI), is underscored by the increasing resistance to ciprofloxacin in Latin America. This issue has led to a heightened dependence on alternative therapeutics, such as cephalosporins. The identification of extended-spectrum β-lactamase (ESBL)-producing E. coli, notably the O25b-ST131 clone, adds complexity to UTI management. The prevalence of ESBL-producing E. coli varies globally due to factors including regional antimicrobial usage practices. Objectives: The goal of this study was to identify and molecularly characterize ESBL-producing E. coli isolates to identify the pandemic O25b-ST131 clone related to UTIs in one healthcare institution in Mexico. Methods: Bacterial species identification and antibiotic susceptibility tests were performed using the VITEK 2. The ESBL genes were identified using polymerase chain reaction (PCR). The E. coli genotyping was carried out by the phylogenetic group analysis and the O25b-ST131 identification. Results: A total of 86 unique E. coli isolates were confirmed as ESBL, and 75% were obtained from UTIs. The most prevalent β-lactamase genes were blaCTX-M (66%), blaTEM (8.1%), blaCTX-M/SHV (5.8%), blaCTX-M/TEM (4.6%), and blaSHV (2.3%). The B2 phylogroup was most prominent (54.4%), with 46.5% identified as a globally pandemic O25b-ST131 clone. No evident relationship was observed using random amplified polymorphic DNA (RAPD) between nosocomial and community-acquired infections in ESBL-producing E. coli isolates. Conclusions: The obtained findings highlight the significance of monitoring molecular epidemiology in antibiotic resistance profiles of the O25b-ST131 E. coli clone.","PeriodicalId":17803,"journal":{"name":"Jundishapur Journal of Microbiology","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2024-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140262504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) causes immune system dysregulation and a systemic cytokine storm. Under healthy conditions, T helper cells protect against intracellular pathogens, extracellular parasites, and extracellular bacteria. Objectives: For the novelty of our study, little is known regarding the balance of T cell subtypes and responses in two forms of COVID-19 in our country. We investigated whether there was a relationship between T cell subtype frequency and cytokines by COVID-19 severity. Methods: Forty-six PCR-confirmed severe (n = 30) and moderate (n = 16) COVID-19 patients and 13 sex- and age-matched healthy control (HC) subjects were enrolled. Immunophenotyping of T cell subsets and related serum cytokines was performed using flow cytometry and ELISA, respectively. Results: There was a significantly lower frequency of CD8+Tbet+ (P < 0.01) T cells in the severe group compared to HC. Also, there was a significantly lower frequency of CD4+GATA3+ (P < 0.001) and CD8+Tbet+ (P < 0.001) T cells in the severe group compared to the moderate group. Moreover, receiver-operating characteristic (ROC) curve analysis revealed a considerable correlation between CTL (CD8+T-bet+) subtypes and the severity of the disease. Severe COVID-19 disease was associated with reduced interferon-gamma (IFN-γ) and interleukin (IL)-2 concentration and increased IL-5 and IL-6 concentration. Conclusions: Reduced systemic levels of IL-2 can trigger decreased numbers of Th1 and Th2 cells, and in contrast to elevated IL-5 and IL-6, the numbers of Th2 cells did not increase in these cases.
背景：严重急性呼吸系统综合征冠状病毒 2（SARS-CoV-2）会导致免疫系统失调和全身细胞因子风暴。在健康状态下，T 辅助细胞能抵御细胞内病原体、细胞外寄生虫和细胞外细菌。研究目的我们的研究很新颖，在我国，人们对两种形式的 COVID-19 中 T 细胞亚型的平衡和反应知之甚少。我们根据 COVID-19 的严重程度调查了 T 细胞亚型频率与细胞因子之间是否存在关系。研究方法纳入 46 名经 PCR 确认的重度（n = 30）和中度（n = 16）COVID-19 患者以及 13 名性别和年龄匹配的健康对照（HC）受试者。分别使用流式细胞术和酶联免疫吸附法对 T 细胞亚群和相关血清细胞因子进行免疫分型。结果显示与 HC 相比，重症组的 CD8+Tbet+ T 细胞频率明显较低（P < 0.01）。此外，与中度组相比，重度组 CD4+GATA3+ (P < 0.001) 和 CD8+Tbet+ (P < 0.001) T 细胞的频率也明显较低。此外，接收器操作特征（ROC）曲线分析显示，CTL（CD8+T-bet+）亚型与疾病严重程度之间存在相当大的相关性。严重的 COVID-19 疾病与γ干扰素（IFN-γ）和白细胞介素（IL）-2 浓度降低以及 IL-5 和 IL-6 浓度升高有关。结论是全身IL-2水平降低会导致Th1和Th2细胞数量减少，与IL-5和IL-6升高相反，Th2细胞数量在这些病例中并未增加。
{"title":"Severity of COVID-19 Infectious and Immune-Based Profile","authors":"H. Shahi, Fatemeh Kiaee, Majid Marjani, E. Mortaz","doi":"10.5812/jjm-143256","DOIUrl":"https://doi.org/10.5812/jjm-143256","url":null,"abstract":"Background: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) causes immune system dysregulation and a systemic cytokine storm. Under healthy conditions, T helper cells protect against intracellular pathogens, extracellular parasites, and extracellular bacteria. Objectives: For the novelty of our study, little is known regarding the balance of T cell subtypes and responses in two forms of COVID-19 in our country. We investigated whether there was a relationship between T cell subtype frequency and cytokines by COVID-19 severity. Methods: Forty-six PCR-confirmed severe (n = 30) and moderate (n = 16) COVID-19 patients and 13 sex- and age-matched healthy control (HC) subjects were enrolled. Immunophenotyping of T cell subsets and related serum cytokines was performed using flow cytometry and ELISA, respectively. Results: There was a significantly lower frequency of CD8+Tbet+ (P < 0.01) T cells in the severe group compared to HC. Also, there was a significantly lower frequency of CD4+GATA3+ (P < 0.001) and CD8+Tbet+ (P < 0.001) T cells in the severe group compared to the moderate group. Moreover, receiver-operating characteristic (ROC) curve analysis revealed a considerable correlation between CTL (CD8+T-bet+) subtypes and the severity of the disease. Severe COVID-19 disease was associated with reduced interferon-gamma (IFN-γ) and interleukin (IL)-2 concentration and increased IL-5 and IL-6 concentration. Conclusions: Reduced systemic levels of IL-2 can trigger decreased numbers of Th1 and Th2 cells, and in contrast to elevated IL-5 and IL-6, the numbers of Th2 cells did not increase in these cases.","PeriodicalId":17803,"journal":{"name":"Jundishapur Journal of Microbiology","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2024-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140445035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
B. Naeimi, Forough Shamsizadeh, Seyed Amin Mohammadi, Gholamreza Khamisipour, Farzaneh Sadeghzadeh, Najmeh Hashemi, B. Ahmadi
Background: Recurrent vulvovaginal candidiasis (RVVC) is a widespread opportunistic gynecological condition resulting from infections by Candida albicans and non-C. albicans (NAC) species. Transforming growth factor-beta (TGF-β), a significant cytokine involved in cell-mediated immunity (CMI), plays a crucial role in vaginal infections. Objectives: The current study was conducted to evaluate the differences in TGF-β gene expression between patients with RVVC and healthy individuals using real-time polymerase chain reaction (PCR). Methods: This case-control study involved 124 patients diagnosed with RVVC and 225 age-matched healthy individuals as controls. The mRNA expression of the TGF-β gene was measured using quantitative real-time PCR (QRT-PCR). Data analysis and the creation of graphs were carried out using SPSS and GraphPad PRISM software. Results: The QRT-PCR findings showed higher TGF-β expression in RVVC patients compared to the control group, though the difference was not statistically significant (P = 0.2538). Conclusions: Our study revealed that the expression of the TGF-β1 isoform was elevated in patients with clinical manifestations in the vagina, although the increase was not statistically significant. Based on this outcome, further in vivo studies are necessary to elucidate the precise role of TGF-β isoforms in the vaginal tract of patients with RVVC.
{"title":"Comparative Analysis of Quantitative Gene Expression of TGF-β in Patients with Recurrent Vulvovaginal Candidiasis and the Normal Population","authors":"B. Naeimi, Forough Shamsizadeh, Seyed Amin Mohammadi, Gholamreza Khamisipour, Farzaneh Sadeghzadeh, Najmeh Hashemi, B. Ahmadi","doi":"10.5812/jjm-140846","DOIUrl":"https://doi.org/10.5812/jjm-140846","url":null,"abstract":"Background: Recurrent vulvovaginal candidiasis (RVVC) is a widespread opportunistic gynecological condition resulting from infections by Candida albicans and non-C. albicans (NAC) species. Transforming growth factor-beta (TGF-β), a significant cytokine involved in cell-mediated immunity (CMI), plays a crucial role in vaginal infections. Objectives: The current study was conducted to evaluate the differences in TGF-β gene expression between patients with RVVC and healthy individuals using real-time polymerase chain reaction (PCR). Methods: This case-control study involved 124 patients diagnosed with RVVC and 225 age-matched healthy individuals as controls. The mRNA expression of the TGF-β gene was measured using quantitative real-time PCR (QRT-PCR). Data analysis and the creation of graphs were carried out using SPSS and GraphPad PRISM software. Results: The QRT-PCR findings showed higher TGF-β expression in RVVC patients compared to the control group, though the difference was not statistically significant (P = 0.2538). Conclusions: Our study revealed that the expression of the TGF-β1 isoform was elevated in patients with clinical manifestations in the vagina, although the increase was not statistically significant. Based on this outcome, further in vivo studies are necessary to elucidate the precise role of TGF-β isoforms in the vaginal tract of patients with RVVC.","PeriodicalId":17803,"journal":{"name":"Jundishapur Journal of Microbiology","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2024-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140448463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Introduction: Vibrio vulnificus (V. vulnificus) is a pathogen that usually induces serious infections and even life-threatening diseases. It might delay the diagnosis and targeted treatment of V. vulnificus infection due to early atypical symptoms and low incidence. Case Presentation: A 73-year-old woman with cerebral infarction developed necrotizing fasciitis with septic infection after admission. The patient developed rapidly progressive symptoms of the right lower limb, nausea and vomiting, hypotension, and liver and kidney function insufficiency on day 10 of hospitalization. Laboratory tests showed a significant increase in infection indicators, and blood cultures showed V. vulnificus growth. Early anti-infective drugs showed a gradual decline in the infection indexes but an aggravation trend in the right lower extremity lesions. The antibiotic regimen was adjusted to cephalosporin combined with quinolones, and local incision decompression and drainage were performed. The patient's infection was controlled, and the local lesion shrank. Conclusions: This case report suggests the importance of referring to the results of drug sensitivity and choosing proper antibiotics in consideration of various factors, such as the infected site of the patient and pharmacokinetics/pharmacodynamics (PK/PD) characteristics of antibiotics for V. vulnificus infection. Meanwhile, the timing of surgical intervention is also crucial for the necrotizing fasciitis caused by V. vulnificus.
{"title":"Sepsis and Necrotizing Fasciitis Caused by Vibrio vulnificus in an Old Woman with Cerebral Infarction","authors":"Weizhen Qiao, Chunyan Chang, Chenglong Liang, Dingye Wu, Xiuhong Zhang","doi":"10.5812/jjm-142631","DOIUrl":"https://doi.org/10.5812/jjm-142631","url":null,"abstract":"Introduction: Vibrio vulnificus (V. vulnificus) is a pathogen that usually induces serious infections and even life-threatening diseases. It might delay the diagnosis and targeted treatment of V. vulnificus infection due to early atypical symptoms and low incidence. Case Presentation: A 73-year-old woman with cerebral infarction developed necrotizing fasciitis with septic infection after admission. The patient developed rapidly progressive symptoms of the right lower limb, nausea and vomiting, hypotension, and liver and kidney function insufficiency on day 10 of hospitalization. Laboratory tests showed a significant increase in infection indicators, and blood cultures showed V. vulnificus growth. Early anti-infective drugs showed a gradual decline in the infection indexes but an aggravation trend in the right lower extremity lesions. The antibiotic regimen was adjusted to cephalosporin combined with quinolones, and local incision decompression and drainage were performed. The patient's infection was controlled, and the local lesion shrank. Conclusions: This case report suggests the importance of referring to the results of drug sensitivity and choosing proper antibiotics in consideration of various factors, such as the infected site of the patient and pharmacokinetics/pharmacodynamics (PK/PD) characteristics of antibiotics for V. vulnificus infection. Meanwhile, the timing of surgical intervention is also crucial for the necrotizing fasciitis caused by V. vulnificus.","PeriodicalId":17803,"journal":{"name":"Jundishapur Journal of Microbiology","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2024-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139836874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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