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Comparison of Quality and Bioactive Compounds in Chicken Thigh Meat from Conventional and Animal Welfare Farm in Korea 韩国传统农场和动物福利农场鸡腿肉品质和生物活性成分的比较
Pub Date : 2018-12-01 DOI: 10.5536/kjps.2018.45.4.261
Hee-jin Kim, Hye-Jin Kim, Jintaek Jeon, Sang-Jip Oh, K. Nam, K. Shim, J. Jung, K. Kim, Y. Choi, Sang-Ho Kim, A. Jang
This study was conducted to evaluate the difference in the quality of chicken thigh meat from conventional and animal welfare farms during refrigeration storage over 9 days. Chicken thigh meat from conventional (CTC, n = 30) and animal welfare farms (CTW, n = 30) was tested. The pH value was significantly lower in CTW (6.28) than in CTC (6.37) on day 1; however, no significant differences were found on subsequent days. The yellowness of CTW was higher than that of CTC on day 1, but CTW showed lower yellowness than did CTC on day 7 and 9. The cooking loss, water holding capacity, lightness, redness, and coliform levels of CTC did not show any significant difference when compared with CTW during storage. The shear force of CTW was significantly higher than that of CTC on day 1, 3, 7, and 9. Total microorganism and coliform in CTC and CTW increased with increasing storage days. On day 7 and 9, the total microorganism level of CTW was lower than that of CTC. The thiobarbituric acid value of CTW was lower than that of CTC on day 9. The volatile basic nitrogen (VBN) of CTW was lower than that of CTC during storage. Anserine content and 1,1-diphenyl-2-picryl-hydrazyl(DPPH) scavenging activity of CTW was significantly higher than CTC on day 1. These results suggest that CTW stayed fresher for longer than did CTC because of low total microorganism level on day 7 and 9, and VBN during refrigerated storage. (
本研究旨在评估来自传统农场和动物福利农场的鸡腿肉在冷藏9天以上的质量差异。选取传统农场(CTC, n = 30)和动物福利农场(CTW, n = 30)的鸡腿肉进行试验。第1天CTW组的pH值(6.28)显著低于CTC组(6.37);然而,在随后的几天里,没有发现明显的差异。第1天CTW黄度高于CTC,第7、9天CTW黄度低于CTC。贮藏期间,CTC的蒸煮损失、保水能力、亮度、红度和大肠菌群水平与CTW相比无显著差异。CTW在第1、3、7、9天的剪切力显著高于CTC。随着贮存天数的增加,CTC和CTW中总微生物和大肠菌群数量均有所增加。第7天和第9天,CTW的微生物总水平低于CTC。第9天CTW的硫代巴比妥酸值低于CTC。在贮藏过程中,CTW的挥发性碱性氮(VBN)低于CTC。第1天,CTW的鹅胺含量和清除1,1-二苯基-2-苦味酰肼(DPPH)的能力显著高于CTC。综上所述,由于CTW在第7天和第9天的总微生物水平较低,而VBN在冷藏过程中比CTC保鲜时间更长。(
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引用次数: 10
Determination of Growth Performance, Viscera Organ Weights and Ileal Intestinal Architecture of Broilers in Response to Drinking Water Added Extractions from Wooden Chips for the Starter Period 肉鸡生长性能、脏器重量和回肠结构对饮水添加木屑提取物的响应
Pub Date : 2018-09-01 DOI: 10.5536/KJPS.2018.45.3.147
S. P. Macelline, S. Kang, Geun Hye Oh, S. Yang, Jeong Bin Nam, J. Hong, H. Cho, Eun-Jib Kim, T. Shin, S. Wickramasuriya, J. Heo
A total of 90 one-day-old male broilers (Ross 308) were randomly assigned to one of the three dietary treatments, each consisting of six replicates (5 broilers/cage). The dietary treatments were 1) control (CON: fresh clean water with no supplement); 2) low dose [LD: CON + 1.56% extractions from the wooden chips (EWC)] and 3) high dose (HD: CON + 12.5% EWC). Drinking water supplemented with EWC was provided using specifically designed individual nipple drinker units. Average daily water intake (ADWI), average daily gain (ADG), average daily feed intake (ADFI), and feed conversion ratio (FCR) were measured weekly for 21 days. One broiler from each cage was euthanized for measuring the visceral organ weights and collecting ileal tissue samples for ileal architecture analysis on day 21. Broilers assigned to the LD and HD watery groups showed higher ADWI than that in broilers consumed CON on day 7 (P<0.05). The broilers subjected to HD treatment showed a deeper crypt depth (P<0.05) than that in broilers subjected to LD and CON on day 21. Therefore, broilers consumed HD showed a lower (P<0.05) villus height:crypt depth ratio than that broilers consumed CON on day 21. Broilers provided drinking water containing any of the tested concentrations of EWC showed no effect (P > 0.05) on growth performance, ileal villus height, and visceral organ weights as compared with those in the CON from hatch to 21 days. In conclusion, broilers fed HD showed reduction in villus height:crypt depth ratio without impairing growth performance and visceral organ weights for the experimental period. (
试验选用90只1日龄雄性肉仔鸡(Ross 308),随机分为3个饲粮处理,每个处理6个重复(5只/笼)。饲粮处理为:1)对照组(CON:不添加淡水);2)低剂量[LD: CON + 1.56%木屑提取液(EWC)]和3)高剂量(HD: CON + 12.5% EWC)。饮用水补充了EWC是由专门设计的单独乳头饮水器单元提供的。每周测定平均日采食量(ADWI)、平均日增重(ADG)、平均日采食量(ADFI)和饲料系数(FCR),连续测定21 d。第21天,每笼1只肉鸡实施安乐死,测量内脏器官重量,收集回肠组织样本进行回肠结构分析。雏鸡从孵化至21 d的生长性能、回肠绒毛高度和内脏器官重量均高于第7天饲喂CON的肉鸡(P < 0.05)。综上所述,饲喂HD降低了肉仔鸡的绒毛高度与隐窝深度比,但不影响其生长性能和内脏器官重量。(
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引用次数: 0
Study on the Characteristics of Feather Developing Pattern and Morphology in Early- and Late-Feathering Korean Native Chickens 韩国土鸡早、晚羽毛发育模式及形态特征的研究
Pub Date : 2018-09-01 DOI: 10.5536/kjps.2018.45.3.155
M. Bang, E. Cho, C. Cho, S. Sohn
Chicken feathers could be classified into early-feathering (EF) and late-feathering (LF) depending on the development and patterns of the wing and tail feathers. Currently, feather-sexing is a widely used chick sexing method in the industry. This study was carried out to suggest the method of classifying of EF and LF chicks to establish auto-sexing Korean native chicken (KNC) strains. The development and morphology of wing feathers and tail feathers in 856 KNCs from hatching to 55-days old were analyzed to classify EF and LF chicks. We also performed PCR analysis using K-specific gene primers to confirm the agreement between the phenotypes and genotypes of EF and LF chickens. In the results, the EF chicks had long primaries and coverts, and there was a significant difference in length between primaries and coverts. The LF chicks had shorter primaries and coverts than the EF chicks, and showed little difference in the length between primaries and coverts. LF chicks could be classified into four groups: LF-Less, LF-Scant, LF-Equal and LF-Reverse according to their wing feather patterns. EF chicks had 1.5 times longer primaries than LF chicks until they were 15-days old, but the lengths were almost the same at 50-days old. The tail feathers of the EF chicks were apparent at 5-days old, but those of the LF chicks were short and indefinite at that time. When EF and LF chicks were classified by the length of primaries being more or less than 9 mm, the classification accuracies for EF and LF chicks were 96.2% and 85.4%, respectively, compared to the PCR results. In conclusion, juvenile EF and LF KNC showed distinct differences in feather development and morphology, and could be easily distinguished at one day-old. (
根据羽翼和尾羽的发育和形态,鸡毛可分为早羽化和晚羽化。目前,羽毛性别鉴定是业界广泛使用的小鸡性别鉴定方法。本研究提出了EF鸡和LF鸡的分类方法,以建立韩国地方鸡(KNC)的自性别菌株。对856只KNCs从孵化到55日龄的翅羽和尾羽的发育和形态进行了分析,对EF和LF雏鸡进行了分类。我们还利用k特异性基因引物进行了PCR分析,以证实EF鸡和LF鸡的表型和基因型之间的一致性。结果表明:EF雏鸡初生和复生体长,初生和复生体长差异显著;低龄雏鸡的初生叶和转生叶长度均较低龄雏鸡短,初生叶和转生叶长度差异不大。根据翅羽特征,可将羽化雏鸡分为少羽化、少羽化、等羽化和反羽化4类。在15日龄之前,EF鸡的初生期长度是LF鸡的1.5倍,但在50日龄时,EF鸡的初生期长度几乎相同。EF鸡的尾羽在5日龄时就很明显,而LF鸡的尾羽较短且不明确。以初生叶长度大于或小于9 mm为分类标准,EF和LF的分类准确率分别为96.2%和85.4%。综上所述,幼鸟EF和LF KNC在羽毛发育和形态上有明显的差异,在1日龄时就可以很容易地区分。(
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引用次数: 6
Effect of Dietary Fermented Garlic Solution on Performance, Egg Quality and Blood Composition in Finishing Period of Laying Hens 饲粮中添加发酵大蒜液对蛋鸡育肥期生产性能、蛋品质和血液成分的影响
Pub Date : 2018-09-01 DOI: 10.5536/KJPS.2018.45.3.201
C. Lim, C. Kang, Hyeon Soo Chun, Ho-Sung Choi, K. Ryu
An experiment was conducted to investigate the effect of fermented garlic solution (FGS) on the performance, egg quality and blood profiles of laying hens in the finishing period. In total, 432 Lohmann Brown hens aged 79 weeks were equally distributed into four dietary treatments with six replicate. Hens were fed the basal diet containing 2,750 kcal/kg of ME and 16% of CP, which was supplemented with either 0% (control), 0.05%, 0.10% and 0.20% FGS from 79 to 83 weeks old. Laying performance, egg quality, yolk fatty acids and serum characteristics were analyzed at the end of experiment. Egg production and feed conversion was numerically improved in FGS supplementation treatments compared to those in the control, but were not statistically different. The albumen height and Haugh unit showed significant increase (P<0.05) in the FGS supplementation groups. The concentration of saturated fatty acid decreased in the yolks of birds fed FGS (P<0.01), whereas the unsaturated fatty acid (UFA) and mono-UFA contents were significantly higher (P<0.01) in those treatments than in the control. Significantly lower natural fat and cholesterol in serum were observed in birds fed the 0.20% FGS supplementation diet (P<0.01). However, the high-density lipoprotein (HDL) cholesterol increased in both the 0.10% and 0.20% FGS supplementation groups. In addition, interleukin-2 mRNA and CD4+/CD8+ level in serum which were cellular immunity indicators showed statistical differences (P<0.01) among treatments and a higher concentration in the 0.10% and 0.20% FGS groups than in the control. Thus, it can be concluded that dietary supplementation of FGS improved egg quality and stimulated immune response in mature laying hens. (
本试验旨在研究发酵大蒜液(FGS)对育肥期蛋鸡生产性能、蛋品质和血液指标的影响。试验选取432只79周龄罗曼布朗母鸡,平均分为4个饲粮处理,每组6个重复。79 ~ 83周龄饲喂代谢能水平为2750 kcal/kg、粗蛋白质水平为16%的基础饲粮,在基础饲粮中分别添加0%(对照)、0.05%、0.10%和0.20%的FGS。试验结束时,对产蛋性能、蛋品质、蛋黄脂肪酸及血清特性进行分析。与对照组相比,添加FGS处理的蛋鸡产蛋量和饲料转化率有数值上的提高,但无统计学差异。各添加组蛋白高度和哈氏单位显著升高(P<0.05)。饲喂FGS的蛋鸡蛋黄中饱和脂肪酸浓度显著降低(P<0.01),不饱和脂肪酸和单不饱和脂肪酸含量显著高于对照组(P<0.01)。饲粮中添加0.20% FGS显著降低了血清中天然脂肪和胆固醇(P<0.01)。然而,添加0.10%和0.20% FGS组的高密度脂蛋白(HDL)胆固醇均升高。血清白细胞介素-2 mRNA和CD4+/CD8+细胞免疫指标各组间差异均有统计学意义(P<0.01),且0.10%和0.20% FGS组血清白细胞介素-2 mRNA和CD4+/CD8+浓度高于对照组。由此可见,饲粮中添加FGS改善了成熟蛋鸡的蛋品质,刺激了免疫反应。(
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引用次数: 0
In Silico Evaluation of Deleterious SNPs in Chicken TLR3 and TLR4 Genes 鸡TLR3和TLR4基因有害单核苷酸多态性的计算机分析
Pub Date : 2018-09-01 DOI: 10.5536/KJPS.2018.45.3.209
Donghyun Shin, K. Song
The innate immune recognition is based on the detection of microbial products. Toll-like receptors (TLRs) located on the cell surface and the endosome senses microbial components and nucleic acids, respectively. Chicken TLRs mediate immune responses by sensing ligands from pathogens, have been studied as immune adjuvants to increase the efficacy of vaccines. Single nucleotide polymorphisms (SNPs) of TLR3 and TLR4 genes in chicken were associated with resistance and susceptibility to viral infection. In this study, SNPs of chTLR3 and chTLR4 genes were retrieved from public database and annotated with chicken reference genome. Three-dimensional models of the chTLR3 and chTLR4 proteins were built using a Swiss modeler. We identified 35 and 13 nsSNPs in chTLR3 and chTLR4 genes respectively. Sorting Intolerant from Tolerant (SIFT) and Polymorphism Phenotyping v2 (Polyphen-2) analyses, suggested that, out of 35 and 13 nsSNPs, 4 and 2 SNPs were identified to be deleterious in chTLR3 and chTLR4 gene respectively. In chTLR3, 1 deleterious SNP was located in ectodomain and 3 were located in the Toll / IL-1 receptor (TIR) domain. Further structural model of chTLR3-TIR domain suggested that 1 deleterious SNP be present in the B-B loop region, which is important for TIR-TIR domain interactions in the downstream signaling. In chTLR4, the deleterious SNPs were located both in the ectodomain and TIR domain. SNPs predicted for chTLR3 and chTLR4 in this study, might be related to resistance or susceptible to viral infection in chickens. Results from this study will be useful to develop the effective measures in chicken against infectious diseases. (
先天免疫识别是以微生物产物的检测为基础的。toll样受体(Toll-like receptor, TLRs)分别位于细胞表面和核内体上,感知微生物成分和核酸。鸡tlr通过感应病原体的配体介导免疫应答,已被研究作为免疫佐剂来提高疫苗的效力。鸡TLR3和TLR4基因的单核苷酸多态性(snp)与病毒感染的抗性和易感性有关。本研究从公共数据库中检索chTLR3和chTLR4基因的snp,并用鸡参考基因组进行注释。使用瑞士建模器构建chTLR3和chTLR4蛋白的三维模型。在chTLR3和chTLR4基因中分别鉴定出35个和13个非单核苷酸多态性。不耐从耐(SIFT)和多态性表型v2 (polyphen2)分析表明,在35个和13个nssnp中,鉴定出4个和2个SNPs分别在chTLR3和chTLR4基因中有害。在chTLR3中,1个有害SNP位于外域,3个位于Toll / IL-1受体(TIR)域。chTLR3-TIR结构域进一步的结构模型表明,在B-B环区存在1个有害SNP,这对下游信号传导中TIR-TIR结构域的相互作用很重要。在chTLR4中,有害snp位于外域和TIR域。本研究预测的chTLR3和chTLR4的snp可能与鸡对病毒感染的抗性或易感性有关。本研究结果将为制定有效的鸡传染性疾病防治措施提供参考。(
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引用次数: 0
A Survey of Korean Consumers' Awareness on Animal Welfare of Laying Hens 韩国消费者对蛋鸡动物福利意识的调查
Pub Date : 2018-09-01 DOI: 10.5536/KJPS.2018.45.3.219
E. Hong, H. Kang, Ki‐Tae Park, Jin-Joo Jeon, Hyunsoo Kim, Chan-ho Kim, Sang-Ho Kim
This study was conducted twice to investigate egg purchase behavior and perception on animal welfare of Korean consumers. This study included women, who were the main decision makers and caretakers in the household, and men with one-person household. This survey was conducted with by the Computer Assisted Web Interview and Gang Survey methods. On the key considerations factor, the highest response rate was considered to be 'price', and the response rate of considering 'packing date' increased in the second survey. At a reasonable price based on 10 eggs, the response rate was the highest at 53.8% and 42.9% in both the first and second surveys and the appropriate price averages were 2,482 won and 2,132 won, respectively. The highest rate of purchase of egg consumers from 'Large Mart' followed by 'Medium sized supermarket' and 'Chain supermarket'. As for the awareness about animal welfare, the recognition ratio (73.5%) was higher in the result of the second survey than the first. The cognitive period of animal welfare was 59.0% before the insecticide egg crisis and 41.0% thereafter. Regarding whether or not they have ever seen an animal welfare certification mark and an animal welfare animal farm certification mark, 59.6% of respondents said that they saw it for the first time and 37.6% answered that they knew the animal welfare certification mark. On the animal welfare system, the 'free-range' response rate was the highest at 85.8%. The 'free-range' fit response decreased by 34.2%p, while the 'barn' and 'European type' fit response increased by 13.2%p and 24.1%p, respectively. The number of 'I have never seen' and 'I have ever eaten' responses to the recognition and eating experience of animal welfare certified eggs decreased while the number of those who answered 'Have ever seen' and 'Have eaten' increased. The answer of purchasing animal welfare certified eggs at department stores, organic farming cooperatives, and internet shopping malls was higher than that of buying conventional eggs. Of the total respondents, 92.0% were willing to purchase an animal welfare egg before the price was offered, but after offering the prices of animal welfare eggs, the intention to purchase was 62.7%, which was about 30%p lower than before. The reason for purchasing an animal welfare certified egg was the highest score of 71.0% for 'I think it is likely to be high in food safety', and 38.1% for 'I think the price is high' for lack of intention to purchase. In the sensory evaluation of animal welfare eggs, egg color and skin texture of conventional eggs were significantly higher than those of certified welfare eggs (P<0.05), and boiled eggs showed that egg whites of animal welfare certified eggs were more (P<0.05). As a result, the results of this study will contribute to the activation of the animal welfare certification system for laying hens by providing basic data on consumer awareness to animal welfare certified farmers. (
本研究对韩国消费者的鸡蛋购买行为和对动物福利的认知进行了两次调查。这项研究包括妇女,她们是家庭中的主要决策者和照顾者,以及只有一人家庭的男子。本调查采用电脑辅助网页访谈及小组调查方法进行。在主要考虑因素中,“价格”的回应率最高,而在第二次调查中,考虑“包装日期”的回应率有所上升。在以10个鸡蛋为标准的“合理价格”调查中,第1次和第2次的回答率分别为53.8%和42.9%,最高,平均价格分别为2482韩元和2132韩元。消费者购买鸡蛋的比例最高的是“大型超市”,其次是“中型超市”和“连锁超市”。在动物福利意识方面,第二次调查结果的认知率(73.5%)高于第一次调查结果。虫卵危机前对动物福利的认知度为59.0%,危机后为41.0%。对于是否见过动物福利认证标志和动物福利动物农场认证标志的问题,59.6%的受访者表示第一次看到,37.6%的受访者表示知道动物福利认证标志。在动物福利制度方面,回答“放养”的比例最高,为85.8%。“自由放养”的匹配反应下降了34.2%,而“谷仓”和“欧式”的匹配反应分别增加了13.2%和24.1%。对动物福利认证鸡蛋的认知度和食用经历,回答“没有见过”和“吃过”的人减少了,回答“见过”和“吃过”的人增加了。在百货店、有机农协、网上购物中心购买动物福利认证鸡蛋的回答比购买普通鸡蛋的回答要高。在所有受访者中,92.0%的人在提供价格之前愿意购买动物福利蛋,但在提供动物福利蛋的价格之后,购买意愿为62.7%,比之前降低了约30个百分点。购买动物福利认证鸡蛋的理由中,“我认为食品安全可能高”的占71.0%,而“我认为价格高”的占38.1%,因为没有购买意愿。在动物福利蛋感官评价中,普通蛋的蛋色和皮质感显著高于认证福利蛋(P<0.05),而煮蛋中,认证动物福利蛋的蛋清更多(P<0.05)。因此,本研究的结果将有助于激活蛋鸡动物福利认证制度,为获得动物福利认证的农民提供消费者意识的基本数据。(
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引用次数: 5
Genetic Diversity and Relationship of Ogye Population in Korea Using 25 Microsatellite Markers 利用25个微卫星标记分析韩国乌溪居群的遗传多样性及亲缘关系
Pub Date : 2018-09-01 DOI: 10.5536/KJPS.2018.45.3.229
H. Roh, Seung-Sook Lee, Jae Yong Han, D. Oh, J. Baek, Sung-Hee Kim, Jun-Heon Lee, Y. Ko, I. Jeon, Seungchang Kim, D. Jeon, Jinwook Lee, Kwan-Woo Kim, C. Cho
The aim of this study was to evaluate the genetic diversity and relationships of Ogye populations in Korea. A total of 243 genomic DNA samples from 6 Ogye population (Yeonsan Ogye; YSO, Animal Genetic Resources Research Center Ogye; ARO, Chungbuk Ogye; CBO, Chungnam Ogye; CNO, Gyeongbuk Ogye; GBO, Seoul National University Ogye; SUO) and 3 introduced chicken breeds (Rhode Island Red; RIR, White Leghorn; LG, Cornish; CN) were used. Sizes of 25 microsatellite markers were decided using GeneMapper Software(v 5.0) after analyzing ABI 3130XL. A total of 153 alleles were observed and the range was 2 to 10 per each locus. The mean of expected and observed heterozygosity and PIC (Polymorphism Information Content) value was 0.53, 0.50, 0.46 respectively. The lowest genetic distance (0.073) was observed between YSO and SUO, and the highest distance (0.937) between the RIR and CBO. The results of clustering analysis suggested 3 clusters (ΔK=7.96). Excluding GBO population, 5 Ogye populations (YSO, ARO, CBO, CNO, SUO) were grouped in same cluster with high genetic uniformity (0.990, 0.979, 0.989, 0.994, 0.985 respectively). But GBO population was grouped in cluster 1 with low genetic uniformity (0.340). The results of this study can be use to basic data for the genetic evaluation and management of Ogye populations in Korea. (
本研究的目的是评价韩国乌溪种群的遗传多样性和亲缘关系。6个Ogye种群(涟山Ogye;Ogye动物遗传资源研究中心;忠北乌溪阿罗;忠南乌溪CBO;庆北Ogye中央军委;首尔大学Ogye GBO;SUO)和3个引进鸡品种(罗德岛红;白里窝那;LG、康沃尔郡的;CN)。分析ABI 3130XL后,采用GeneMapper软件(v 5.0)确定25个微卫星标记的大小。共检测到153个等位基因,每个位点2 ~ 10个。期望杂合度和观测杂合度的平均值分别为0.53、0.50和0.46。YSO与SUO的遗传距离最低(0.073),RIR与CBO的遗传距离最高(0.937)。聚类分析结果为3类(ΔK=7.96)。除GBO群体外,5个Ogye群体(YSO、ARO、CBO、CNO、SUO)聚在同一聚类中,遗传均匀度分别为0.990、0.979、0.989、0.994、0.985。而GBO群体聚在第1类,遗传均匀性较低(0.340)。本研究结果可为韩国乌溪种群的遗传评价和管理提供基础数据。(
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引用次数: 1
Utilization of Processed Pine Needle Powder in Broiler Chicken Diets 加工松针粉在肉鸡饲粮中的利用
Pub Date : 2018-09-01 DOI: 10.5536/KJPS.2018.45.3.167
Eun-Jib Kim, J. Jung, H. Cho, S. Wickramasuriya, Shemil Pyrian Maceline, T. Shin, Beomgyu Kim, J. Heo
This study examined the effects of dietary processed pine needle tree powder (PPNP) on growth performance, visceral organ parameters, ileal digestibility, blood metabolites and ileal morphology of broiler chickens for 5-week after hatch. In total, 220 1-day-old male Ross 308 broiler chicks were randomly allocated to 30 pens (seven birds per pen), and each pen was assigned to one of five dietary treatments, a diet without PPNP (control) and diets with 0.5%, 1.0%, 1.5% and 2.0% PPNP. The body weight and feed intake were measured weekly. One bird from each pen was selected randomly and gently euthanized to collect the blood, visceral organs and digesta samples on 2, 3 and 5 week of age. Birds fed 2.0% PPNP showed decreased (P<0.05) overall body weight, average daily gain and average daily feed intake compared to those fed control, 0.5 and 1.0% PPNP. The small intestine length increased (P<0.05) as the level of PPNP in the diet increased. A deeper crypt depth was observed (P<0.05) when birds fed 2.0% PPNP compared to those fed the control diet at week 2. A higher V:C ratio was observed (P<0.05) in birds fed 1.0% PPNP than in those fed other treatments at week 3. Ileal protein digestibility improved (P<0.05) when birds fed 0.5% and 1.0% PPNP compared to that in those fed the control diet at week 3. The feed conversion ratio, ileal dry matter digestibility and blood urea nitrogen and cholesterol, and gizzard and ceca weights were not affected (P>0.05) by supplementation of any levels of PPNP. These results demonstrated that inclusion of PPNP in broiler diets could be a possible factor in improving overall growth performance suggesting early gut development when 1% PPNP is supplemented. (
本试验旨在研究饲粮中添加加工松针粉(PPNP)对孵化后5周肉鸡生长性能、内脏器官参数、回肠消化率、血液代谢产物和回肠形态的影响。试验选用220只1日龄罗斯308肉鸡雄性,随机分配到30个栏中(每个栏7只),每个栏分别饲喂不添加PPNP的饲粮(对照)和添加0.5%、1.0%、1.5%和2.0% PPNP的饲粮。每周测定体重和采食量。在2、3、5周龄时,每栏随机选取1只,轻轻安乐死,采集血液、内脏器官和食糜样本。饲粮中添加2.0%的PPNP后,各组PPNP均显著降低(P0.05)。这些结果表明,在肉仔鸡饲粮中添加PPNP可能是提高整体生长性能的一个可能因素,这表明当添加1% PPNP时,肉鸡的肠道发育会提前。(
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引用次数: 1
Effect of Dietary Lactobacillus, Medicinal Plants Extract, Herb Extract on the Laying Performance, Cecal Microflora, Relative Organs Weight, Leucocyte Profiles in Laying Hens against Salmonella gallinarum 饲粮中添加乳酸菌、药用植物提取物、草药提取物对蛋鸡产蛋性能、盲肠菌群、相对脏器重量和白细胞谱对鸡沙门氏菌的影响
Pub Date : 2018-09-01 DOI: 10.5536/KJPS.2018.45.3.183
H. Kang, S. Park, Hyun Soo Kim, Jin-Joo Jeon, K. Park, C. Kim, E. Hong, Sang-Ho Kim
The study was conducted to investigate the effect of dietary supplementation of Lactobacillus, medicinal plants extract, and essential oil on laying performance, cecal microflora, relative organic weight, leucocyte profiles in laying hens against Salmonella gallinarum. A total of 100 Hy-Line Brown laying hens of 65 weeks of age were assigned into 5 dietary treatments supplemented with no antibiotic (negative control), antibiotic (positive control), Lactobacillus 0.1% (LB), medicinal plants extract 100 ppm (MPE), herb extract 100 ppm (EO). After 7 days of treatment, the 20 hens in each treatment group were divided into challenge group (n=10) and non-challenge group (n=10) for oral administration of Salmonella gallinarums, and were maintained in the same dietary treatments for two additional weeks. Laying hens were selected 20 by each treatment, splitting up into oral Salmonella group and nontreated group. The feeding trial lasted for one weeks and Salmonella gallinarum was orally administered for two weeks. Egg production, egg weight, egg mass, and feed conversion ratio were calculated and cecal microflora, relative organ, and blood were collected at the end of experiment. Compared with the control, laying hens fed Lactobacillus, medical plant extract, and essential oil were not observed to change of laying performance. Salmonella count increased significantly when Salmonella gallinarum was orally administered (P<0.05) but coliform bacteria and Lactobacillus counts were not affected. The relative organs weight was not different in hens with oral administration of Salmonella gallinarum and also in hens fed Lactobacillus, medical plant extract, and essential oil. H/L ratio was not significantly different, but total leucocyte level was somewhat increased in the treatment with oral administration of Salmonella gallinarum and more leucocyte increased in laying hens fed Lactobacillus, medical plant extract, and essential oil than control. (
本试验旨在研究饲粮中添加乳酸菌、药用植物提取物和精油对蛋鸡产蛋性能、盲肠菌群、相对有机重和白细胞分布对鸡沙门氏菌的影响。选取100只65周龄的海兰褐蛋鸡,分为5个饲粮处理,分别添加无抗生素(阴性对照)、抗生素(阳性对照)、0.1%乳酸杆菌(LB)、100 ppm药用植物提取物(MPE)、100 ppm草本植物提取物(EO)。处理7 d后,每个处理组20只鸡分为攻毒组(n=10)和非攻毒组(n=10),分别口服鸡沙门氏菌,并在相同的饲粮处理下再维持2周。每个处理选取20只蛋鸡,分为口服沙门氏菌组和未处理组。饲养试验1周,口服鸡沙门氏菌2周。计算产蛋量、蛋重、蛋质量和饲料系数,并在试验结束时采集盲肠菌群、相关器官和血液。与对照组相比,饲喂乳酸菌、药用植物提取物和精油对蛋鸡产蛋性能无显著影响。口服鸡沙门菌组沙门氏菌数量显著增加(P<0.05),而大肠菌群和乳酸菌数量未受影响。口服鸡沙门菌组和饲喂乳酸菌、药用植物提取物和精油组的相对脏器重量无显著差异。H/L比值差异不显著,但总白细胞水平在口服鸡沙门菌组有所升高,且乳杆菌组、植物提取物组和精油组的白细胞升高幅度大于对照组。(
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引用次数: 0
Comparative Analysis of Meat Quality Traits of New Strains of Native Chickens for Samgyetang 参鸡汤土鸡新品种肉质性状的比较分析
Pub Date : 2018-09-01 DOI: 10.5536/KJPS.2018.45.3.175
Sung-Yun Lee, Ji-young Park, Jung-Min Hyun, Samooel Jung, C. Jo, K. Nam
The carcass and meat quality of new native chicken strains (A, C, and D) being developed in the Golden Seed Project for Samgyetang were compared with a commercial native chicken (Hanhyup No. 3, H) and Baeksemi (white semi broiler, W) strains. In total, 250 male chicks were raised under the same feeding and raising conditions for 5 weeks. After slaughter, the carcass weight and percentage yield of parts from each strain were measured and the physicochemical quality traits (general composition, color values, fatty acid composition, and sensory evaluation) of breast muscles were comparatively analyzed. The carcass weight (875.10 g) of the W strain was significantly heavier than that of the other native chicken strains, while that of A strain (537.54 g) was the lowest. The percentage yield of breast meat was also higher in the W strain than in the other strains. The fat content of W strain was higher than that of the other native chicken strains. The new native chicken strains (A, C, D) had higher contents of linoleic acid (C18:2) than the H strain. The A and D strains especially showed relatively high content of arachidonic acid (C20:4), a characteristic fatty acid in native chicken meats. On the other hand, the fatty acid composition of C strain was similar to that of the W strain. This study provides important information on specific quality characteristics than can be used to select new native chicken lines for breeding program. (
对三溪堂金种子项目开发的新土鸡品系A、C、D与市售土鸡(韩协3号,H)和白半肉鸡(白半肉鸡,W)的胴体和肉质进行了比较。在相同饲养条件下,共饲养250只雄性雏鸡5周。屠宰后测定各品系胴体重和各部位产率,比较分析胸肌的理化品质性状(一般组成、色值、脂肪酸组成、感官评价)。W品系的胴体重(875.10 g)显著高于其他地方鸡品系,而A品系的胴体重(537.54 g)最低。W菌株的胸肉产率也高于其他菌株。W菌株的脂肪含量高于其他地方鸡菌株。新土鸡菌株(A、C、D)的亚油酸(C18:2)含量高于H菌株。特别是A和D菌株,其花生四烯酸(C20:4)的含量较高,这是土鸡肉中特有的脂肪酸。另一方面,C菌株的脂肪酸组成与W菌株相似。本研究提供了重要的品质性状信息,可用于选育新的土鸡品系。(
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引用次数: 12
期刊
Korean Journal of Poultry Science
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