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A Survey on Egg Laying Performance and Distribution Status of Animal Welfare Certified Farms for Laying Hens 动物福利认证养殖场蛋鸡产蛋性能及分布状况调查
Pub Date : 2019-06-01 DOI: 10.5536/KJPS.2019.46.2.55
E. Hong, H. Kang, K. Park, Jin-Joo Jeon, Hyunsoo Kim, Chan-ho Kim, Sang-Ho Kim
This study was conducted to evaluate animal welfare approved farms in three housing systems (open, windowless, and free-range). The survey was conducted in 25 animal welfare approved farms, and 10 farms were surveyed for distribution status. The main breed in all animal welfare approved farms of laying hens was Hy-Line Brown variety. In the case of open house, laying hens were bred in traditional and panel houses simultaneously; however, the ratio of panel house was 58.3%, which was higher than that of the traditional house. All the windowless houses were made of panels and more than 15,000 laying hens were housed in a single windowless house. In the case of free-range house, it was maintained on a small scale of less than 12,000 birds. Fifty-six percent of the surveyed farms were breeding at 7∼8 birds/m. In terms of male and female ratios, most farms maintained 1 male:15 females, but there was a farmhouse that switched 17 or 20 females to 1 male. The daily dietary allowance was 110∼170 g, and 32% of the surveyed farms provided feed of more than 150 g/day, which showed that forage feed was important. The age of at the first egg was 123 days, 122 days, and 120 days, and the peak percent was 91.8%, 94.9%, and 86.5% in open, windowless and free-range houses, respectively. The average egg production rate was 74.0%, 84.6%, and 72.7% in open, windowless, and free-range houses respectively, thus, there was no correlation between feed intake and hen-housed eggs. Distribution of welfare certified eggs was mainly a direct deal with the consumer or through contract production. The ratio of direct transactions between large-scale marts and eco-friendly specialty stores of welfare approved eggs was higher than that of conventional eggs. The rate of contract sales of eggs in both the barn and free-range systems was high, and the percentage of courier sales farms was also high. Excluding courier services, price of eggs in the barn system rose to more than 30 won/egg in the second half of 2017 (after AI). Price of eggs in the free-range system rose to more than 50 won/egg in the second half of 2017 (after AI). In the case of courier sales, the same price of 500 won was maintained before and after AI. In conclusion, the results of this study can be used as basic data for improving the animal welfare certification system for laying hens in Korea. (
本研究对三种住房系统(开放式、无窗和散养)的动物福利农场进行了评估。该调查在25个动物福利认证农场进行,并调查了10个农场的分布状况。所有动物福利批准养殖场的蛋鸡品种主要为海兰褐品种。在开放式鸡舍中,蛋鸡在传统鸡舍和板式鸡舍中同时饲养;而板式住宅的比例为58.3%,高于传统住宅。所有的无窗房屋都是用嵌板建造的,在一间无窗房屋里饲养了15000多只蛋鸡。在自由放养的情况下,它的规模很小,只有不到1.2万只鸟。56%的养殖场以7 ~ 8只/m的速度繁殖。就男女比例而言,大多数农场维持1名男性:15名女性,但有一个农舍将17或20名女性变为1名男性。日饲料允许量为110 ~ 170 g, 32%的受访养殖场提供的饲料超过150 g/d,这表明草料饲料的重要性。开窗、无窗和散养鸡舍的首蛋龄分别为123日、122日和120日,最高率分别为91.8%、94.9%和86.5%。开放式鸡舍、无窗鸡舍和散养鸡舍的平均产蛋率分别为74.0%、84.6%和72.7%,采食量与母鸡舍蛋无关。福利认证鸡蛋的分销主要是与消费者直接交易或通过合同生产。福利认证鸡蛋在大型超市和环保专卖店之间的直接交易比率高于普通鸡蛋。在禽舍和散养系统中,合同销售鸡蛋的比例都很高,快递销售农场的比例也很高。除快递服务外,2017年下半年(人工智能之后)畜舍系统的鸡蛋价格上涨到了30韩元以上。2017年下半年(人工智能之后),散养鸡蛋的价格上涨到了50韩元以上。以快递销售为例,AI之前和之后的价格都维持在500韩元左右。综上所述,本研究结果可作为完善韩国蛋鸡动物福利认证制度的基础数据。(
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引用次数: 0
Quality Characteristics of Samgyetang with Medicinal Herbs 中药参鸡汤的品质特征
Pub Date : 2019-06-01 DOI: 10.5536/KJPS.2019.46.2.95
Samooel Jung, Tae-Kyung Kim, Su-Kyung Ku, H. Yong, Kyung-Woo Lee, Young-Boong Kim, Yun-Sang Choi
This study investigated the effect of traditional medicinal plants on the quality characteristics of Samgyetang breast meat and broth. The traditional medicinal plants used in this study were roots of Panax ginseng, Codonopsis lanceolata, Platycodon grandiflorum, Salvia miltiorrhiza, Adenophora triphylla, and Allium hookeri. There was no significant difference (P>0.05) in the moisture, protein, ash content, water holding capacity, and cooking loss of Samgyetang meat with the addition of traditional medicinal plants. The color values (lightness, redness, and yellowness) of Samgyetang meat and broth were significantly different (P<0.05); the 2-thiobarbituric acid reactive substances and shear force of Samgyetang meat were significantly different (P<0.05); and the pH, turbidity, and viscosity of Samgyetang broth were also significantly different (P<0.05) with the addition of traditional medicinal plants. In the overall acceptability of Samgyetang meat, the addition of S. miltiorrhiza showed the highest score compared to that of other treatments (P<0.05). Thus, the addition of traditional medicinal plants in the production of Samgyetang were found to affect the lipid rancidity beneficially with plant species when compared to Samgyetang with Panax ginseng. In conclusion, with the development of Samgyetang using S. miltiorrhiza, it will be possible to develop products with superior quality characteristics in antioxidant, shear force, and overall acceptability. (
研究了传统药用植物对参鸡汤肉汤品质特性的影响。本研究使用的传统药用植物为人参根、苍参根、桔梗根、丹参根、三叶参根、葱根。添加传统药材对参鸡汤肉的水分、蛋白质、灰分含量、持水量和蒸煮损失均无显著影响(P>0.05)。参鸡汤肉与肉汤的颜色值(亮度、红度、黄度)差异显著(P<0.05);参鸡汤肉的2-硫代巴比妥酸活性物质和剪切力差异显著(P<0.05);中药对参鸡汤的pH、浊度、黏度也有显著影响(P<0.05)。在参鸡汤肉的总体接受度上,丹参添加组得分最高(P<0.05)。因此,与人参参鸡汤相比,在参鸡汤中加入传统药用植物对脂质酸败有有益的影响。综上所述,利用丹参开发参鸡汤,将有可能开发出在抗氧化性、剪切力和整体可接受性方面具有优良品质特征的产品。(
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引用次数: 0
Genetic Diversity of Korean Native Chicken Populations in DAD-IS Database Using 25 Microsatellite Markers 利用25个微卫星标记分析DAD-IS数据库中韩国地方鸡群体的遗传多样性
Pub Date : 2019-06-01 DOI: 10.5536/KJPS.2019.46.2.65
H. Roh, Kwan-Woo Kim, Jinwook Lee, D. Jeon, Seungchang Kim, Y. Ko, Seong-Sil Mun, Hyunjoo Lee, Jun-Heon Lee, D. Oh, Jae-Hyun Byeon, C. Cho
A number of Korean native chicken(KNC) populations were registered in FAO (Food and Agriculture Organization) DAD-IS (Domestic Animal Diversity Information Systems, http://www.fao.org/dad-is). But there is a lack of scientific basis to prove that they are unique population of Korea. For this reason, this study was conducted to prove KNC’s uniqueness using 25 Microsatellite markers. A total of 548 chickens from 11 KNC populations (KNG, KNB, KNR, KNW, KNY, KNO, HIC, HYD, HBC, JJC, LTC) and 7 introduced populations (ARA: Araucana, RRC and RRD: Rhode Island Red C and D, LGF and LGK: White Leghorn F and K, COS and COH: Cornish brown and Cornish black) were used. Allele size per locus was decided using GeneMapper Software (v 5.0). A total of 195 alleles were observed and the range was 3 to 14 per locus. The MNA, Hexp, Hobs, PIC value within population were the highest in KNY (4.60, 0.627, 0.648, 0.563 respectively) and the lowest in HYD (1.84, 0.297, 0.286, 0.236 respectively). The results of genetic uniformity analysis suggested 15 cluster (ΔK = 66.22). Excluding JJC, the others were grouped in certain cluster with high genetic uniformity. JJC was not grouped in certain cluster but grouped in cluster 2 (44.3%), cluster 3 (17.7%) and cluster8 (19.1%). As a results of this study, we can secure a scientific basis about KNC’s uniqueness and these results can be use to basic data for the genetic evaluation and management of KNC breeds. (
一些韩国本土鸡(KNC)种群已在粮农组织(FAO) DAD-IS(家畜多样性信息系统,http://www.fao.org/dad-is)登记。但是没有科学依据证明它们是韩国特有的种群。因此,本研究利用25个微卫星标记来证明KNC的独特性。试验选用11个KNC群体(KNG、KNB、KNR、KNW、KNY、KNO、HIC、HYD、HBC、JJC、LTC)和7个引进群体(ARA: Araucana、RRC和RRD:罗德岛红C和D、LGF和LGK:白来角F和K、COS和COH:康沃尔棕和康沃尔黑)共548只鸡。采用GeneMapper软件(v 5.0)测定每个位点的等位基因大小。共检测到195个等位基因,每个位点3 ~ 14个。种群内MNA、Hexp、Hobs、PIC值以KNY最高,分别为4.60、0.627、0.648、0.563,HYD最低,分别为1.84、0.297、0.286、0.236。遗传均匀性分析结果为15个聚类(ΔK = 66.22)。除JJC外,其余均聚在一定的聚类中,遗传均匀性较高。JJC不属于特定的聚类,而是属于聚类2(44.3%)、聚类3(17.7%)和聚类8(19.1%)。研究结果为KNC品种的独特性提供了科学依据,为KNC品种的遗传评价和管理提供了基础数据。(
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引用次数: 2
Effect of Hatching and Brooding Season of Chicks on Their Heat Stress Response and Production Performances 孵蛋季节对雏鸡热应激反应及生产性能的影响
Pub Date : 2019-06-01 DOI: 10.5536/kjps.2019.46.2.77
E. Cho, E. Choi, S. Sohn
This study was conducted to compare the heat stress response and production performance of chicks hatched in winter and summer. Among the 2,090 Korean native chickens examined, 1,156 hatched in winter and 934 hatched in summer. The amount of telomeric DNA, the expression of heat shock protein (HSP) genes, survival rate, egg production, and body weight were analyzed to evaluate the stress response and production performance of chickens. The results showed that the expression of HSP-70, HSP-90α, and HSP-90β genes in the winter-hatched chickens were significantly higher than those in the summer-hatched chickens during the growing and laying period (P<0.05). There was no significant difference in the amount of telomeric DNA between summerand winter-hatched chickens. The survival rate was significantly higher in the summer-hatched chickens than in the winter-hatched chickens at the laying period (P<0.01). The hen-day egg production and egg weight in the summer-hatched chickens were also significantly higher than those in the winter-hatched chickens (P<0.05). In contrast, age of sexual maturity of winter-hatched chickens was significantly earlier than that of summer-hatched chickens (P<0.01). The body weights from birth to 24 weeks were significantly lighter in the summer-hatched chickens than in the winter-hatched chickens, however, it was reversed after 28 weeks (P<0.05). In conclusion, the chicks hatched in the summer are more resistant to heat stress, with better productivity than the chicks hatched in the winter. These results suggest that the chicks grown at high temperatures have greater adaptability to the thermal environment. (
本试验旨在比较冬季和夏季孵化雏鸡的热应激反应和生产性能。在调查的2090只韩国本土鸡中,冬季孵出的有1156只,夏季孵出的有934只。通过分析端粒DNA的数量、热休克蛋白(HSP)基因的表达、成活率、产蛋量和体重来评价鸡的应激反应和生产性能。结果表明:在生长和产蛋期,冬孵鸡的热休克蛋白70、热休克蛋白90α和热休克蛋白90β基因的表达量显著高于夏孵鸡(P<0.05)。夏孵鸡和冬孵鸡端粒DNA数量无显著差异。产蛋期夏孵鸡的成活率极显著高于冬孵鸡(P<0.01)。夏孵鸡的母鸡日产蛋量和蛋重也显著高于冬孵鸡(P<0.05)。冬孵鸡的性成熟年龄显著早于夏孵鸡(P<0.01)。出生至24周夏孵鸡的体重显著低于冬孵鸡,28周夏孵鸡的体重与冬孵鸡相反(P<0.05)。综上所述,夏季孵化的雏鸡比冬季孵化的雏鸡更能抵抗热应激,产蛋率更高。这些结果表明,在高温条件下生长的雏鸡对热环境具有更强的适应性。(
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引用次数: 3
Multiple Genotypes of Avian Infectious Bronchitis Virus Circulating in Vietnam 越南流行的禽传染性支气管炎病毒的多基因型
Pub Date : 2019-06-01 DOI: 10.5536/kjps.2019.46.2.127
T. Le, H. Lee, V. Le, Kang-Seuk Choi
Three strains of infectious bronchitis viruses (IBVs), designated VNUA3, VNUA8 and VNUA11, were isolated from diseased/infected chickens in Hanoi, Thainguyen, and Haiphong provinces of Vietnam. These birds had received a live IBV vaccination but still suffered from infectious bronchitis. VNUA3, VNUA8 and VNUA11 harbor cleavage sites (RRTGR, HRRRR, and HRRKR, respectively) within the S protein. A BLASTN search revealed that the S gene of VNUA3, VNUA8, and VNUA11 showed the highest nucleotide identity with those of IBV strains CK/Italy/I2022/13, CK/CH/LHLJ/08-6, and GX-NN120084, respectively. Phylogenetic analyses based on the S gene nucleotide sequences revealed that VNUA3, VNUA8 and VNUA11 clustered with Q1-like, QX-like and TC07-2-like genotypes, respectively, and were closely related to reference IBV strains from China. However, the Vietnam IBVs showed high divergence from vaccine strains 4/91 and Ma5, which are used in the Vietnamese farms from which the isolates were obtained. Taken together, these results indicate that at least three genotypes of IBV are circulating among chickens in North Vietnam. This is the first report of the molecular epidemiology of IBV in Vietnam. (
从越南河内、丹庆和海防三省病鸡中分离到3株传染性支气管炎病毒(ibv),分别为VNUA3、VNUA8和VNUA11。这些鸟接受了IBV活疫苗接种,但仍然患有传染性支气管炎。VNUA3、VNUA8和VNUA11在S蛋白中含有裂解位点(分别为RRTGR、HRRRR和HRRKR)。BLASTN检索结果显示,VNUA3、VNUA8和VNUA11分别与IBV株CK/Italy/I2022/13、CK/CH/LHLJ/08-6和GX-NN120084的S基因核苷酸同源性最高。基于S基因核苷酸序列的系统进化分析显示,VNUA3、VNUA8和VNUA11分别为Q1-like、QX-like和TC07-2-like基因型聚类,与中国IBV参考株亲缘关系密切。然而,越南ibv与获得分离株的越南农场使用的疫苗株4/91和Ma5表现出高度差异。综上所述,这些结果表明至少有三种基因型的IBV在越南北部的鸡群中传播。这是越南首次报道IBV分子流行病学。(
{"title":"Multiple Genotypes of Avian Infectious Bronchitis Virus Circulating in Vietnam","authors":"T. Le, H. Lee, V. Le, Kang-Seuk Choi","doi":"10.5536/kjps.2019.46.2.127","DOIUrl":"https://doi.org/10.5536/kjps.2019.46.2.127","url":null,"abstract":"Three strains of infectious bronchitis viruses (IBVs), designated VNUA3, VNUA8 and VNUA11, were isolated from diseased/infected chickens in Hanoi, Thainguyen, and Haiphong provinces of Vietnam. These birds had received a live IBV vaccination but still suffered from infectious bronchitis. VNUA3, VNUA8 and VNUA11 harbor cleavage sites (RRTGR, HRRRR, and HRRKR, respectively) within the S protein. A BLASTN search revealed that the S gene of VNUA3, VNUA8, and VNUA11 showed the highest nucleotide identity with those of IBV strains CK/Italy/I2022/13, CK/CH/LHLJ/08-6, and GX-NN120084, respectively. Phylogenetic analyses based on the S gene nucleotide sequences revealed that VNUA3, VNUA8 and VNUA11 clustered with Q1-like, QX-like and TC07-2-like genotypes, respectively, and were closely related to reference IBV strains from China. However, the Vietnam IBVs showed high divergence from vaccine strains 4/91 and Ma5, which are used in the Vietnamese farms from which the isolates were obtained. Taken together, these results indicate that at least three genotypes of IBV are circulating among chickens in North Vietnam. This is the first report of the molecular epidemiology of IBV in Vietnam. (","PeriodicalId":17845,"journal":{"name":"Korean Journal of Poultry Science","volume":"39 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76263683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 6
Effect of Persimmon Peel as an Additional Feeding 柿皮补饲的效果
Pub Date : 2019-06-01 DOI: 10.5536/KJPS.2019.46.2.87
Sang-Hee Shin, Junkoo Yi, C. Kong, W. Kwon, Z. Ryoo, Eungyung Kim, Hyeonjin Kim, J. Ha, Doyoung Hong, D. Yoon, Sunghyun Kim, M. Kim
Huge amount of persimmon peels, which are byproducts usually arising after making dried persimmons from fresh persimmons, are generated in fall, every year. As the dry matter of persimmon peel contains carbohydrates over 80%, it could be a good candidate for feed. In this study, we evaluated the persimmon peel as a feed resource for a hen laying eggs. Persimmon peel has lower ratio of crude protein and fat composition while higher ratio of crude fiber composition compared to a basal laying hen feed. Feeding the persimmon peel by adding to the feed at the level of 2% or 5% did not affect the egg-laying rate from beginning to the peak of laying. In addition, feeding the persimmon peel did not affect the egg quality either, as the pH, Haugh unit, yolk color and shell thickness of the egg were not different from each other. The composition of amino acids and fatty acids were also almost same for each other, except for the linolenic acid which was slightly higher in the persimmon peel feeding groups. In conclusion, addition of persimmon peel to the feed up to 5% might be helpful to increase the income and reduce the waste produced from the agricultural byproduct. (
每年秋天都会产生大量的柿皮,这是新鲜柿子制成柿干后产生的副产品。柿皮干物质碳水化合物含量达80%以上,可作为饲料的优良原料。在本研究中,我们对柿皮作为母鸡下蛋的饲料资源进行了评价。与基础蛋鸡饲料相比,柿皮的粗蛋白质和脂肪组成比较低,粗纤维组成比较高。在饲料中添加2%或5%的柿皮饲喂,从产蛋开始到产蛋高峰期对产蛋率没有影响。另外,饲喂柿皮对鸡蛋的pH、哈氏单位、蛋黄颜色和蛋壳厚度也没有影响。除亚麻酸含量在柿皮饲喂组略高外,氨基酸和脂肪酸的组成也基本相同。综上所述,在饲料中添加5%的柿皮有利于提高饲料收入,减少农副产物的浪费。(
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引用次数: 0
Production Performance of 12 Korean Domestic Chicken Varieties Preserved as National Genetic Resources 作为国家遗传资源保存的12个韩国家鸡品种的生产性能
Pub Date : 2019-06-01 DOI: 10.5536/KJPS.2019.46.2.105
K. Kim, E. Choi, J. Kwon, Hyun Chul Jung, S. Sohn
In this study, viability, growth performance and egg production performance were investigated to determine the productive characteristics of 12 Korean domestic chicken varieties which have been collected and conserved for over 25 years in National Institute of Animal Science, Rural Development Administration, Korea. The 1,134 hens were tested and their production traits including survival rate, body weight, age at first egg laying, hen-day and hen-housed egg production, egg weight, and egg quality were measured. Survival rate was the highest in Korean Rhode-D and Korean Native Chicken (KNC) White and the lowest in Korean Cornish Brown (92.2% and 54.3%, respectively). Body weights from 4 to 50 wks were consistently high in the order of Korean Cornish, Korean Rhode, KNC, Korean Ogye, and Korean Leghorn breeds. There was no significant difference in weight between varieties within a breed. Age at first egg laying was the shortest in Korean Leghorn, while it was the longest in Korean Cornish. The hen-day egg production from 1 egg to 57 wks was the highest in Korean Leghorn-K, and the lowest in Korean Cornish Brown. Egg weight was the heaviest in Korean Leghorn-F and the lightest in KNC White. The Haugh unit was the highest in Korean Rhode-C and the lowest in Korean Ogye. Taken together, these results suggest that it is desirable that the Korean Cornish variety is improved as the Korean meat breed because of its excellent growth ability, the Korean Leghorn variety is improved as the Korean layer breed because of its good laying performance, and the Korean Rhode and KNC varieties are improved as strong viable breeds because of their good survival rate. (
本研究对12个韩国家鸡品种进行了活力、生长性能和产蛋性能的研究,以确定这些品种在韩国农村发展管理局国家动物科学研究所收集和保存了25年以上的生产特性。试验选用1134只母鸡,测定其成活率、体重、初产蛋龄、母鸡日产蛋量和母鸡舍产蛋量、蛋重和蛋品质等生产性状。成活率最高的是韩国罗德白和韩国土鸡(KNC)白,最低的是韩国康沃尔布朗(分别为92.2%和54.3%)。从4到50周,韩国康沃尔犬、韩国罗德犬、KNC犬、韩国Ogye犬和韩国Leghorn犬的体重一直很高。在一个品种内,不同品种之间的体重没有显著差异。初产蛋龄朝鲜族里格霍恩最短,而朝鲜族康沃尔最长。从1个蛋到57周的母鸡日产蛋量以韩国来斤- k最高,而韩国康沃尔布朗最低。蛋重以韩国来鸿f最重,KNC白最轻。哈夫单位在韩国语罗德- c中最高,在韩国语Ogye中最低。综上所述,韩国康沃尔品种因其优良的生长能力而被改良为韩国肉用品种,韩国来斤品种因其良好的产蛋性能而被改良为韩国蛋鸡品种,韩国罗德和KNC品种因其良好的成活率而被改良为强活产品种。(
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引用次数: 9
토종닭 농장주 및 가공업자가 바라본 토종닭 산업의 미래 방향 土鸡农场主及加工者眼中土鸡产业的未来方向
Pub Date : 2019-03-01 DOI: 10.5536/kjps.2019.46.1.47
J. Yoon, Hyun Jung Lee, Ki-Chang Nam, Cheorun Jo
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引用次数: 0
Effects of Dietary Bee Venom on Serum Characteristic, Antioxidant Activity and Liver Fatty Acid Composition in Broiler Chickens 饲粮中添加蜂毒对肉鸡血清特性、抗氧化活性和肝脏脂肪酸组成的影响
Pub Date : 2019-03-01 DOI: 10.5536/KJPS.2019.46.1.39
Da-Hye Kim, S. Han, Yun-Sang Choi, H. Kang, Hong-Gu Lee, Kyung-Woo Lee
This study was conducted to investigate the effect of dietary bee venom on serum characteristics, antioxidant activity, and hepatic fatty acid composition in broiler chickens. A group of 875 one-day-old feather-sexed male broiler chicks were randomly allocated to five treatments with seven replicates (25 birds/replicate) for three weeks. A corn-soybean meal-based diet was used as the basal diet. Five dietary treatments were compared: 1) basal diet, 2) basal diet containing 10 μg/kg of bee venom powder, 3) basal diet containing 50 μg/kg of bee venom powder, 4) basal diet containing 100 μg/kg of bee venom powder, and 5) basal diet containing 500 μg/kg of bee venom powder. At 21 days, one bird per pen was slaughtered by asphyxiation in CO2 gas, and blood was collected to measure serum characteristics and antioxidant activity. In addition, the liver was excised to measure the concentration of malondialdehyde and determine fatty acid composition. Increasing dietary bee venom in the diet failed to affect most serum parameters except for triglyceride and non-esterified fatty acids. Dietary bee venom inclusion quadratically increased the concentration of stearic acid (P<0.05), but decreased palmitoleic acid, oleic acid, linoleic acid, mono-unsaturated fatty acids, and poly-unsaturated fatty acids. Finally, dietary bee venom tended to lower hepatic malondialdehyde contents quadratically (P=0.054). In conclusion, our study revealed that dietary bee venom improved antioxidant capacity and affected fatty acid metabolism in broiler chickens. (
本试验旨在研究饲粮中添加蜂毒对肉鸡血清特性、抗氧化活性和肝脏脂肪酸组成的影响。试验选用1日龄羽性雄性肉仔鸡875只,随机分为5个处理,每组7个重复(25只/重复),试验期3周。以玉米-豆粕为基础日粮。比较5种饲粮处理:1)基础饲粮、2)基础饲粮中蜂毒粉的添加量为10 μg/kg、3)基础饲粮中蜂毒粉的添加量为50 μg/kg、4)基础饲粮中蜂毒粉的添加量为100 μg/kg、5)基础饲粮中蜂毒粉的添加量为500 μg/kg。21 d时,每栏1只鸡在CO2气体中窒息屠宰,采集血液测定血清特征和抗氧化活性。此外,切除肝脏以测定丙二醛浓度并测定脂肪酸组成。在饲料中增加蜂毒对除甘油三酯和非酯化脂肪酸外的大多数血清参数没有影响。饲料中加入蜂毒可二次提高硬脂酸浓度(P<0.05),降低棕榈油酸、油酸、亚油酸、单不饱和脂肪酸和多不饱和脂肪酸浓度(P<0.05)。最后,饲料中添加蜂毒有二次降低肝脏丙二醛含量的趋势(P=0.054)。综上所述,饲粮中添加蜂毒可提高肉鸡抗氧化能力,影响脂肪酸代谢。(
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引用次数: 7
Analysis of Transcriptional Activity and Estrogen Responsiveness of Regulatory Elements in Chicken Ovalbumin Promoter 鸡卵白蛋白启动子调控元件的转录活性及雌激素响应性分析
Pub Date : 2019-03-01 DOI: 10.5536/KJPS.2019.46.1.17
Hyeon Yang, Kyungwoon Kim, J. Kim, J. Woo, Hwi-Cheul Lee, Hoonsung Choi, Sun Keun Jung, S. Sureshkumar, Haesun Lee, K. Oh, S. Byun
Chickens have been considered as well-defined animal bioreactor. The optimized ovalbumin promoter is essential for recombinant protein production in transgenic chicken. Here we try to compare the activity and identify the effect of estrogen on ovalbumin promoter according to each promoter length with estrogen response element (ERE) existence. We cloned two (2.8 and 5.5 kb) ovalbumin promoters that the 5.5 kb contained the ERE but the 2.8 kb did not, and these two promoters were cloned to pGL4.11 vector. Additionally, we constructed another pGL4.11 vector containing of the 4.4 kb (with ERE) ovalbumin promoter deleted with 1 kb between ERE region and the 2.8 kb promoter. For reporter assay, HeLa, MES-SA, LMH/2A, and cEF cells were transfected with all the pGL4.11 vectors. The comparative analysis showed that the mutated 4.4 kb promoter has more potent activity than the 2.8 and 5.5 kb promoters in HeLa, MES-SA, and LMH/2A cells. However, there is no significant difference in cEFs. Also, these cells transfected with the mutated 4.4 kb promoter were treated with the 17β -estradiol (0∼3,000 nM) and HeLa, MES-SA, and LMH/2A cells showed estrogen responsibilities, but cEFs did not. Besides, the mutated 4.4 kb promoter has still higher activity than the 2.8 and 5.5 kb promoter, and there is no transcriptional induction effect in 2.8 kb promoter at 500 nM estrogen that is blood concentration of laying hens. Hence our study strongly suggested that the mutated 4.4 kb promoter is considered as one of the most efficient length for generating transgenic chicken. (
鸡一直被认为是一种定义良好的动物生物反应器。优化后的卵白蛋白启动子是转基因鸡生产重组蛋白的必要条件。本文根据不同启动子长度是否存在雌激素反应元件(estrogen response element, ERE),比较了雌激素对卵清蛋白启动子的活性和作用。我们克隆了两个卵白蛋白启动子(2.8 kb和5.5 kb),其中5.5 kb的启动子含有ERE,而2.8 kb的启动子不含ERE,并将这两个启动子克隆到pGL4.11载体上。此外,我们构建了另一个pGL4.11载体,该载体包含4.4 kb(含ERE)卵清蛋白启动子,在ERE区域和2.8 kb启动子之间缺失了1 kb。在报告细胞实验中,用所有pGL4.11载体转染HeLa、MES-SA、LMH/2A和cEF细胞。对比分析表明,在HeLa、MES-SA和LMH/2A细胞中,突变的4.4 kb启动子比2.8和5.5 kb启动子具有更强的活性。然而,cEFs没有显著差异。同样,这些转染了突变4.4 kb启动子的细胞用17β -雌二醇(0 ~ 3,000 nM)处理,HeLa, MES-SA和LMH/2A细胞显示出雌激素的责任,但cEFs没有。此外,突变4.4 kb启动子的活性仍然高于2.8和5.5 kb启动子,并且在500 nM雌激素(即蛋鸡血药浓度)下,2.8 kb启动子没有转录诱导作用。因此,我们的研究强烈表明,突变的4.4 kb启动子被认为是产生转基因鸡最有效的长度之一。(
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Korean Journal of Poultry Science
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