Pub Date : 2021-06-30DOI: 10.36326/kjvs/2021/v12i13222
Haneen B. Jaddoua, K. Khudair
This study was designed to investigate the ameliorative role of gallic acid (GA) and N-acetylcysteine (NAC) in reducing deleterious effect of sodium fluoride (NaF) such as, oxidative stress and hepatic dysfunction in adult male rats. Thirty adult male rats were randomly and equally divided into five groups, they were handled daily for 60 days, as follows: Control group (C), received tap water only, Sodium fluoride group (T1), received 100ppm of NaF in drinking tap water, gallic acid group (T2), rats in this group were injected intraperitoneal (i/p) 150 mg/kg/day/ of GA, N-acetylcysteine group (T3), animals in this group were administrated orally 25 mg/kg/day/of NAC, while the combination of GA and NAC were given to NaF treated group(T4) in the same previous mentioned doses and method of administration. Fasting blood sample were collected at the beginning and the end of the experiment and serum were collected for estimation of hepatic enzymes concentration and antioxidant status. After animal scarifying, samples from hepatic tissue were taken for measuring hepatic reduced glutathione (GSH)and malondialdehyde (MDA) concentration. The results showed that adminstration of NaF (T1group) caused hepatic damage manifested functionally by: significant increase in serum alanine aminotransferase (ALT) and alkaline phosphatase (ALP) concentrations, a case of oxidative stress as explained by depression in (GSH) and elevation in MDA concentration in serum and hepatic tissue. The current result also recorded that i/p injection of GA oral administration of NAC alone or in combination with NaF caused amelioration of all previously estimated parameters.
{"title":"Role of N-acetyl cysteine and gallic acid or their combination in Some criteria related to hepatic damage in sodium fluoride treated rats","authors":"Haneen B. Jaddoua, K. Khudair","doi":"10.36326/kjvs/2021/v12i13222","DOIUrl":"https://doi.org/10.36326/kjvs/2021/v12i13222","url":null,"abstract":"This study was designed to investigate the ameliorative role of gallic acid (GA) and N-acetylcysteine (NAC) in reducing deleterious effect of sodium fluoride (NaF) such as, oxidative stress and hepatic dysfunction in adult male rats. Thirty adult male rats were randomly and equally divided into five groups, they were handled daily for 60 days, as follows: Control group (C), received tap water only, Sodium fluoride group (T1), received 100ppm of NaF in drinking tap water, gallic acid group (T2), rats in this group were injected intraperitoneal (i/p) 150 mg/kg/day/ of GA, N-acetylcysteine group (T3), animals in this group were administrated orally 25 mg/kg/day/of NAC, while the combination of GA and NAC were given to NaF treated group(T4) in the same previous mentioned doses and method of administration. Fasting blood sample were collected at the beginning and the end of the experiment and serum were collected for estimation of hepatic enzymes concentration and antioxidant status. After animal scarifying, samples from hepatic tissue were taken for measuring hepatic reduced glutathione (GSH)and malondialdehyde (MDA) concentration. The results showed that adminstration of NaF (T1group) caused hepatic damage manifested functionally by: significant increase in serum alanine aminotransferase (ALT) and alkaline phosphatase (ALP) concentrations, a case of oxidative stress as explained by depression in (GSH) and elevation in MDA concentration in serum and hepatic tissue. The current result also recorded that i/p injection of GA oral administration of NAC alone or in combination with NaF caused amelioration of all previously estimated parameters.","PeriodicalId":17869,"journal":{"name":"Kufa Journal For Veterinary Medical Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83457328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-12-31DOI: 10.36326/KJVS/2020/0110204
Anas Almousawi, A. O. Alhatami
Background: Staphylococcus aureus (S. aureus) causes a difficult problem in the poultry industry because it causes diseases that are difficult to treat due to the resistance of these bacteria to antibiotics and their possession of a battery of virulence and resistance genes in addition to their ability to produce thick biofilms. Method: A cross-sectional study conducted to collect a total of 53 samples from different clinical cases in broilers during the period from August 2019 to February 2020 in Al-Najaf and Karbala cities, The clinical isolates were determined by using the conventional standard biochemical tests. All the specimens cultured on blood agar medium supplemented with 5% blood for primary isolation and selected by using selective media mannitol salt agar (MSA) for confirmation the mannitol fermentation, then subjected to gram’s staining, catalase, oxidase, and further slide coagulase test, then all S. aureus isolates tested by antibiotic susceptibility test, and screened for the presence of mecA and mecC genes using PCR for the detection of MRSA isolates, then subjected to the detection of virulence genes (pvl and eta), antibiotic resistance gene (cfr), identification of integron class 1, biofilm formation assay, the multi-druge resistance profiles (MDR) and multible antibiotics resistance (MAR) indexes were calculated. Results: the isolation rate of S. aureus from the broilers' clinical samples was 37.7%. The antibiotic susceptibility test revealed that 85% of S. aureus isolates were resistant to one or more of the antibiotic tested. All 53 isolates were assessed for the presence of mecA and mecC genes by using PCR. The mecA gene-specific PCR product was seen in 7 (35%) isolates and considered as MRSA. Among all S. aureus isolates, two isolates were positive for the eta gene, and 15 (75%) isolates harboring integron class 1, while the biofilm formation test revealed that 7 (35%) was positive biofilm producers and three of them were strong producers, consequentlly, 13 (65%) of the isolates were resisted to three or more antibiotics and considered as MDR strains. While pvl, cfr, and mecC gene were not detected among S. aureus isolates. Conclusion: the current study revealed that S. aureus possess a real threat in the poultry industry reflecting a public health problem due to the large acquisition of antibiotic resistance genes by these bacteria, the results indicated a high percentage of isolates having MDR characteristic, and two of them were resistant to all antibiotics tested. In addition to the presence of two MRSA isolates carrying the eta gene, this indicating that they are of human origin.
{"title":"Isolation and molecular characterization of staphylococcus aureus isolated from clinical cases in broilers","authors":"Anas Almousawi, A. O. Alhatami","doi":"10.36326/KJVS/2020/0110204","DOIUrl":"https://doi.org/10.36326/KJVS/2020/0110204","url":null,"abstract":"Background: Staphylococcus aureus (S. aureus) causes a difficult problem in the poultry industry because it causes diseases that are difficult to treat due to the resistance of these bacteria to antibiotics and their possession of a battery of virulence and resistance genes in addition to their ability to produce thick biofilms. Method: A cross-sectional study conducted to collect a total of 53 samples from different clinical cases in broilers during the period from August 2019 to February 2020 in Al-Najaf and Karbala cities, The clinical isolates were determined by using the conventional standard biochemical tests. All the specimens cultured on blood agar medium supplemented with 5% blood for primary isolation and selected by using selective media mannitol salt agar (MSA) for confirmation the mannitol fermentation, then subjected to gram’s staining, catalase, oxidase, and further slide coagulase test, then all S. aureus isolates tested by antibiotic susceptibility test, and screened for the presence of mecA and mecC genes using PCR for the detection of MRSA isolates, then subjected to the detection of virulence genes (pvl and eta), antibiotic resistance gene (cfr), identification of integron class 1, biofilm formation assay, the multi-druge resistance profiles (MDR) and multible antibiotics resistance (MAR) indexes were calculated. Results: the isolation rate of S. aureus from the broilers' clinical samples was 37.7%. The antibiotic susceptibility test revealed that 85% of S. aureus isolates were resistant to one or more of the antibiotic tested. All 53 isolates were assessed for the presence of mecA and mecC genes by using PCR. The mecA gene-specific PCR product was seen in 7 (35%) isolates and considered as MRSA. Among all S. aureus isolates, two isolates were positive for the eta gene, and 15 (75%) isolates harboring integron class 1, while the biofilm formation test revealed that 7 (35%) was positive biofilm producers and three of them were strong producers, consequentlly, 13 (65%) of the isolates were resisted to three or more antibiotics and considered as MDR strains. While pvl, cfr, and mecC gene were not detected among S. aureus isolates. Conclusion: the current study revealed that S. aureus possess a real threat in the poultry industry reflecting a public health problem due to the large acquisition of antibiotic resistance genes by these bacteria, the results indicated a high percentage of isolates having MDR characteristic, and two of them were resistant to all antibiotics tested. In addition to the presence of two MRSA isolates carrying the eta gene, this indicating that they are of human origin.","PeriodicalId":17869,"journal":{"name":"Kufa Journal For Veterinary Medical Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88234686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-12-31DOI: 10.36326/KJVS/2020/0110203
Anas Almousawi, A. O. Alhatami, Furkan Alaraji
The ability of Staphylococcus aureus (S. aureus) to acquire variety and many virulence genes that leads to its the ability to cause different diseases in many hosts species, these bacteria have many mechanisms for antibiotic resistance and their ability to produce biofilm and gain various genes through integrons, that will lead to difficult treatment. Consequently, the acquisition of the mecA gene made it more virulent and resistant to antibiotics, and this indicates that the spread of these bacteria in human and animal communities besides health institutions and their frequent transmission between these communities may have a problem of dealing with it.
{"title":"An overview: the virulence capacity, resistance mechanism, integron association, biofilm formation ability, and methicillin- resistance traits among staphylococcus aureus","authors":"Anas Almousawi, A. O. Alhatami, Furkan Alaraji","doi":"10.36326/KJVS/2020/0110203","DOIUrl":"https://doi.org/10.36326/KJVS/2020/0110203","url":null,"abstract":"The ability of Staphylococcus aureus (S. aureus) to acquire variety and many virulence genes that leads to its the ability to cause different diseases in many hosts species, these bacteria have many mechanisms for antibiotic resistance and their ability to produce biofilm and gain various genes through integrons, that will lead to difficult treatment. Consequently, the acquisition of the mecA gene made it more virulent and resistant to antibiotics, and this indicates that the spread of these bacteria in human and animal communities besides health institutions and their frequent transmission between these communities may have a problem of dealing with it.","PeriodicalId":17869,"journal":{"name":"Kufa Journal For Veterinary Medical Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83227466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-12-30DOI: 10.36326/KJVS/2020/0110202
Ahmed Rasool, A. H. Al-Dabhawi
This study has been conducted to examine the macroscopic and histopathologic lesions of liver in slaughtered cattle at Al-Najaf City, and then the prevalence of these lesions has been listed. Liver of two hundred cattle slaughtered at the city slaughterhouse of Al-Najaf were collected from beginning of January to the end of March, 2019 for gross and microscopic investigations. Tissue processing has been carried out for specimens inspected as tissue lesions were examined under light microscopy. The results of the macroscopic and microscopic survey showed that there were variable liver affections in slaughtered cattle. The lesions were arranged from high percentages of lesions such as fasciolosis (32%) and abscesses (32.5%), to moderate to low percentages such as hydatidosis (19.5%), tuberculosis (7.5%), fatty degeneration (3%) and coagulative necrosis (1.5%). The main conclusion of our study revealed that there were high prevalence of liver lesions in slaughtered cattle in Al-Najaf slaughterhouse (4.69%), that lead to liver condemnation and economic losses.
{"title":"Pathological investigation of liver lesions in slaughtered cattle in Al-Najaf abattoir","authors":"Ahmed Rasool, A. H. Al-Dabhawi","doi":"10.36326/KJVS/2020/0110202","DOIUrl":"https://doi.org/10.36326/KJVS/2020/0110202","url":null,"abstract":"This study has been conducted to examine the macroscopic and histopathologic lesions of liver in slaughtered cattle at Al-Najaf City, and then the prevalence of these lesions has been listed. Liver of two hundred cattle slaughtered at the city slaughterhouse of Al-Najaf were collected from beginning of January to the end of March, 2019 for gross and microscopic investigations. Tissue processing has been carried out for specimens inspected as tissue lesions were examined under light microscopy. The results of the macroscopic and microscopic survey showed that there were variable liver affections in slaughtered cattle. The lesions were arranged from high percentages of lesions such as fasciolosis (32%) and abscesses (32.5%), to moderate to low percentages such as hydatidosis (19.5%), tuberculosis (7.5%), fatty degeneration (3%) and coagulative necrosis (1.5%). The main conclusion of our study revealed that there were high prevalence of liver lesions in slaughtered cattle in Al-Najaf slaughterhouse (4.69%), that lead to liver condemnation and economic losses.","PeriodicalId":17869,"journal":{"name":"Kufa Journal For Veterinary Medical Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78806213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-12-30DOI: 10.36326/KJVS/2020/0110206
R. Aziz, E. Mohameed
Objective(s): P. mirabilis is considered as extensive drug resistant pathogen in many studies as it can resist complex antibiotic regimes, such pathogen can be threat to public health, especially when it has the ability to produce biofilm. Therefore, biofilm production was characterized among XDR P. mirabilis local isolates in this research. Material and Methods: 100 P. mirabilis isolated from wound infections from patients admitted to Baghdad hospitals. They were identified using biochemical test and Vitek2 system. The MIC test for antibiotic sensitivity was done by Vitek 2 automated system. Biofilm production was identified phenotypically by twitching motility test, scanning electron microscope, and microtiter plate assay. Results: It could be revealed that 8/100 isolates were MDR, 90/100 isolates were XDR and pan drug resistance level was shown in only two isolates. 80% of isolates had motile ability through twitching assay, and scan electron microscope study revealed that 76% of XDR isolates could produce different stages of biofilm on coverslip placed in MacConkey broth. Microtiter plate assay revealed 81% of XDR isolates were biofilm producers. Conclusion: it could be concluded that extensive drug resistant P. mirabilis can produce biofilm hence resist several important antibiotics; making treatment of infection among wounded patients is such a challenge in many hospitals in Baghdad.
{"title":"Characterization of Biofilm production in XDR Proteus mirabilis isolated from Baghdad city hospitals","authors":"R. Aziz, E. Mohameed","doi":"10.36326/KJVS/2020/0110206","DOIUrl":"https://doi.org/10.36326/KJVS/2020/0110206","url":null,"abstract":"Objective(s): P. mirabilis is considered as extensive drug resistant pathogen in many studies as it can resist complex antibiotic regimes, such pathogen can be threat to public health, especially when it has the ability to produce biofilm. Therefore, biofilm production was characterized among XDR P. mirabilis local isolates in this research. Material and Methods: 100 P. mirabilis isolated from wound infections from patients admitted to Baghdad hospitals. They were identified using biochemical test and Vitek2 system. The MIC test for antibiotic sensitivity was done by Vitek 2 automated system. Biofilm production was identified phenotypically by twitching motility test, scanning electron microscope, and microtiter plate assay. Results: It could be revealed that 8/100 isolates were MDR, 90/100 isolates were XDR and pan drug resistance level was shown in only two isolates. 80% of isolates had motile ability through twitching assay, and scan electron microscope study revealed that 76% of XDR isolates could produce different stages of biofilm on coverslip placed in MacConkey broth. Microtiter plate assay revealed 81% of XDR isolates were biofilm producers. Conclusion: it could be concluded that extensive drug resistant P. mirabilis can produce biofilm hence resist several important antibiotics; making treatment of infection among wounded patients is such a challenge in many hospitals in Baghdad.","PeriodicalId":17869,"journal":{"name":"Kufa Journal For Veterinary Medical Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86348465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-12-30DOI: 10.36326/KJVS/2020/0110205
Dher Al-Haidary, Mohammad Naqi
The present study has been designed to evaluate the potent role of healing efficacy of lanolin (which is also called wool wax), is an oily product secreted by the sebaceous glands beneath the skin of sheep which is have anti-inflammatory, anti-microbial, skin protecting properties, a forty-eight mature albino rats (weighted 250-350 g) were randomly assigned to two groups; control group and eczema group which is induced by 99.9% pure acetone. By squad cotton and put it on shaved skin, after 3 days of acetone application the eczema group were divided randomly into two equal groups each group 16 rats, treat these 32 rats with glycerin and lanolin, after 24h five rats from each group were sacrificed, and remaining rats were treated with glycerin and lanolin for 72h and then sacrificed at the third day, the histopathology there is a lot of histopathological changes occur in tissue section in eczematous skin in comparison with control section; there were obvious thickness and enlargement in the length of outermost layer of skin, also there is fissures and cracks in the epidermal layer due to the dryness of skin and aggregation of inflammatory cells. And as well as keratinization of epithelial layer and changes in skin color, the results indicate there were optimum healing effect of lanolin group in compare with glycerin group after 72h, Showed back the outer most layer of the skin to its normal length, also decrease in inflammatory cells number.
{"title":"Comparative histophysiological study the effective treatment of induced eczema in rats that treated with Lanolin and glycerine with Vaseline in rats","authors":"Dher Al-Haidary, Mohammad Naqi","doi":"10.36326/KJVS/2020/0110205","DOIUrl":"https://doi.org/10.36326/KJVS/2020/0110205","url":null,"abstract":"The present study has been designed to evaluate the potent role of healing efficacy of lanolin (which is also called wool wax), is an oily product secreted by the sebaceous glands beneath the skin of sheep which is have anti-inflammatory, anti-microbial, skin protecting properties, a forty-eight mature albino rats (weighted 250-350 g) were randomly assigned to two groups; control group and eczema group which is induced by 99.9% pure acetone. By squad cotton and put it on shaved skin, after 3 days of acetone application the eczema group were divided randomly into two equal groups each group 16 rats, treat these 32 rats with glycerin and lanolin, after 24h five rats from each group were sacrificed, and remaining rats were treated with glycerin and lanolin for 72h and then sacrificed at the third day, the histopathology there is a lot of histopathological changes occur in tissue section in eczematous skin in comparison with control section; there were obvious thickness and enlargement in the length of outermost layer of skin, also there is fissures and cracks in the epidermal layer due to the dryness of skin and aggregation of inflammatory cells. And as well as keratinization of epithelial layer and changes in skin color, the results indicate there were optimum healing effect of lanolin group in compare with glycerin group after 72h, Showed back the outer most layer of the skin to its normal length, also decrease in inflammatory cells number.","PeriodicalId":17869,"journal":{"name":"Kufa Journal For Veterinary Medical Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83004172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-12-30DOI: 10.36326/KJVS/2020/0110201
Razzaq Baiee, A. Saeed
The current study was conducted in the Animal field of the Department of Animal Production – College of Agriculture - Al-Qasim Green University for the period from 10/10/2019 to 19/1/2020 to investigate the effect of addition of monensin at levels of 15, 30 and 45 mg/kg concentrate on growth performance of Awassi lambs. First treatment in which lambs were fed concentrate diet without addition of monensin was considered the control treatment. Sixteen male Awassi lambs were used with an average weight of 24.85 and 4-6 months of age. The lambs were randomly distributed to the experimental treatments, four lambs per each using the individual feeding in the cages. The concentrate diet was offered at a level of 2.75% of the body weight at 2 meals, morning and evening meals, while the ground wheat straw was offered at free choice. Results revealed no significant effect of level of monensin on pH values, whereas ruminal ammonia concentrations were significantly decreased (P˂0.05) from 7.54 in the control treatment to 6.08 and 6.45 mg/100 ml due to addition of monensin at levels of 30 and 45 mg/kg. Moreover, addition of monensin at a level of 30 mg/kg concentrate diet significantly increased (P˂0.05) ruminal concentration of total volatile fatty acids to 13.76 as compared with 10.99 mM/100 ml for control treatment. Concentrations of all rumen fermentation characteristics were significantly affected (P˂0.01) by time of ensiling.
{"title":"Effect of level of monensin on rumen fermentation characteristics in Awassi lambs","authors":"Razzaq Baiee, A. Saeed","doi":"10.36326/KJVS/2020/0110201","DOIUrl":"https://doi.org/10.36326/KJVS/2020/0110201","url":null,"abstract":"The current study was conducted in the Animal field of the Department of Animal Production – College of Agriculture - Al-Qasim Green University for the period from 10/10/2019 to 19/1/2020 to investigate the effect of addition of monensin at levels of 15, 30 and 45 mg/kg concentrate on growth performance of Awassi lambs. First treatment in which lambs were fed concentrate diet without addition of monensin was considered the control treatment. Sixteen male Awassi lambs were used with an average weight of 24.85 and 4-6 months of age. The lambs were randomly distributed to the experimental treatments, four lambs per each using the individual feeding in the cages. The concentrate diet was offered at a level of 2.75% of the body weight at 2 meals, morning and evening meals, while the ground wheat straw was offered at free choice. Results revealed no significant effect of level of monensin on pH values, whereas ruminal ammonia concentrations were significantly decreased (P˂0.05) from 7.54 in the control treatment to 6.08 and 6.45 mg/100 ml due to addition of monensin at levels of 30 and 45 mg/kg. Moreover, addition of monensin at a level of 30 mg/kg concentrate diet significantly increased (P˂0.05) ruminal concentration of total volatile fatty acids to 13.76 as compared with 10.99 mM/100 ml for control treatment. Concentrations of all rumen fermentation characteristics were significantly affected (P˂0.01) by time of ensiling.","PeriodicalId":17869,"journal":{"name":"Kufa Journal For Veterinary Medical Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86919330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-06-30DOI: 10.36326/kjvs/2020/v11i13299
Muhammad Ali Hameed Jalil, N. M. Al-Sharafi
The study was designed to evaluated exocrine pancreatic enzyme replacement therapy in hyperglycemic male rats induced by alloxan. A total of forty five adult male rats were use in this study, hyperglycemia was induce in thirty rats by single intraperitoneal (i.p) injection of Alloxan 100mg/kg/B.W. and the experimental (45) distributed into three groups: the first group (15 animals) administered distilled water orally service as control group. The second group hyperglycemic groups (15 animals) was treated with exocrine pancreatic enzyme(EPE).The third group hyperglycemic group non treated with exocrine pancreatic enzyme(NEPE). Blood samples were take in days 20,40,60 of experiment for measurement the following parameters: Serum some biochemical parameters, tissue pancreatic activity. The results showed that tissue orally gavages exocrine pancreatic enzyme(EPE) caused a significant elevation in tissue amylase activity in all treated group as compared to the control group. and significant increase in tissue amylase and decrease lipase and protease in concentration in EPE treated group as compared to the non-treated (NEPE) group. Also serum insulin concentration significant decrease and glucose showed a significant increase in EPE and NEPE groups when compared with the control group in after 20,40 and 60 day of experiment. The serum albumin showed significant decrease in all EPE groups when contrasted with the control group. And also The results illustrated that significant decrease in albumin concentration in all NEPE as compared to the control group. The Serum protein concentration was significant decrease in EPE and NEPE groups as compared to the control group in all experimental day. The results showed significant increase in serum cholesterol and triglyceride in EPE as compared to the NEPE . The results showed that PEP rats caused a significant decrease in serum high density lipoprotein (HDL)concentration as compared to the control group and significant decrease in HDL concentration in NEPE as compared to the control group. results showed that doses rats caused a significant(P
{"title":"Pancreatic Enzyme Replacement Therapy in hyperglycemic Male Rats","authors":"Muhammad Ali Hameed Jalil, N. M. Al-Sharafi","doi":"10.36326/kjvs/2020/v11i13299","DOIUrl":"https://doi.org/10.36326/kjvs/2020/v11i13299","url":null,"abstract":"The study was designed to evaluated exocrine pancreatic enzyme replacement therapy in hyperglycemic male rats induced by alloxan. A total of forty five adult male rats were use in this study, hyperglycemia was induce in thirty rats by single intraperitoneal (i.p) injection of Alloxan 100mg/kg/B.W. and the experimental (45) distributed into three groups: the first group (15 animals) administered distilled water orally service as control group. The second group hyperglycemic groups (15 animals) was treated with exocrine pancreatic enzyme(EPE).The third group hyperglycemic group non treated with exocrine pancreatic enzyme(NEPE). Blood samples were take in days 20,40,60 of experiment for measurement the following parameters: Serum some biochemical parameters, tissue pancreatic activity. \u0000The results showed that tissue orally gavages exocrine pancreatic enzyme(EPE) caused a significant elevation in tissue amylase activity in all treated group as compared to the control group. and significant increase in tissue amylase and decrease lipase and protease in concentration in EPE treated group as compared to the non-treated (NEPE) group. Also serum insulin concentration significant decrease and glucose showed a significant increase in EPE and NEPE groups when compared with the control group in after 20,40 and 60 day of experiment. The serum albumin showed significant decrease in all EPE groups when contrasted with the control group. And also The results illustrated that significant decrease in albumin concentration in all NEPE as compared to the control group. The Serum protein concentration was significant decrease in EPE and NEPE groups as compared to the control group in all experimental day. The results showed significant increase in serum cholesterol and triglyceride in EPE as compared to the NEPE . The results showed that PEP rats caused a significant decrease in serum high density lipoprotein (HDL)concentration as compared to the control group and significant decrease in HDL concentration in NEPE as compared to the control group. results showed that doses rats caused a significant(P","PeriodicalId":17869,"journal":{"name":"Kufa Journal For Veterinary Medical Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83632641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-06-30DOI: 10.36326/kjvs/2020/v11i13301
Zainab Alaulddin M. Hussien, N. M. Al-Sharafi
The study designed to evaluate influence of kisspeptin on polycystic ovary syndrome in female rats, First experiment (Induction of polycystic ovary): thirtieth virgin female rats divided randomly into two groups first group via used ten rats serves as control group . While the second group twenty female rat (PCOS-induce group) were orally administered with letrozole at a dose of 1 mg/kg/21 days. The Second Experiment Effect of treatments with kisspeptin on PCOS : Animals were divided as follows: first group (negative control) .Second group (Positive control) .While animals in third and fourth groups (PCOS+Kisspeptin): five animal in each group PCOS-induced rats administrated of 20 and 40 nmol/rat S/C 21 days Kisspeptin daily respectively .The result show in first experiment a significant increase in serum activity of LH, FSH, estrogen and testosterone and significant decrease progesteron concentration in serum of female rats treated with letrozole. While in the second experiment there was significant decrease in FSH in the C+ compared with C and PCOS treated group. A significant increase in the LH in C+ compared with C and treated group 20 and 40 nmol/rat Kisspeptin . Also the results showed that there was a significant decrease in serum estrogen concentration was observed in PCOS nontreated group compared with control. On other hand a significant increase in serum estrogen in T1(20 nmol/kg B.W) compared with T2(40 nmol/kg B.W). The results of the histopathological study of ovary after treatment PCOS with kisspeptin 20 nmol/kg show partial return of follicular cyst to normal ovarian tissue, but the treated with 40 nmol/kg show semicomplete treated and return follicular cyst to normal ovarian tissue. On conclusions, the present study confirmed that PCOS affect the female reproductive hormonal balance and kisspeptin 40 nmol/rat daily S/C injection show effect in normal restoring of female reproductive hormonal and histological balance of PCOS rats.
{"title":"Treatment of polycystic ovary syndrome in rats by kisspeptin","authors":"Zainab Alaulddin M. Hussien, N. M. Al-Sharafi","doi":"10.36326/kjvs/2020/v11i13301","DOIUrl":"https://doi.org/10.36326/kjvs/2020/v11i13301","url":null,"abstract":"The study designed to evaluate influence of kisspeptin on polycystic ovary syndrome in female rats, First experiment (Induction of polycystic ovary): thirtieth virgin female rats divided randomly into two groups first group via used ten rats serves as control group . While the second group twenty female rat (PCOS-induce group) were orally administered with letrozole at a dose of 1 mg/kg/21 days. The Second Experiment Effect of treatments with kisspeptin on PCOS : Animals were divided as follows: first group (negative control) .Second group (Positive control) .While animals in third and fourth groups (PCOS+Kisspeptin): five animal in each group PCOS-induced rats administrated of 20 and 40 nmol/rat S/C 21 days Kisspeptin daily respectively .The result show in first experiment a significant increase in serum activity of LH, FSH, estrogen and testosterone and significant decrease progesteron concentration in serum of female rats treated with letrozole. While in the second experiment there was significant decrease in FSH in the C+ compared with C and PCOS treated group. A significant increase in the LH in C+ compared with C and treated group 20 and 40 nmol/rat Kisspeptin . Also the results showed that there was a significant decrease in serum estrogen concentration was observed in PCOS nontreated group compared with control. On other hand a significant increase in serum estrogen in T1(20 nmol/kg B.W) compared with T2(40 nmol/kg B.W). The results of the histopathological study of ovary after treatment PCOS with kisspeptin 20 nmol/kg show partial return of follicular cyst to normal ovarian tissue, but the treated with 40 nmol/kg show semicomplete treated and return follicular cyst to normal ovarian tissue. On conclusions, the present study confirmed that PCOS affect the female reproductive hormonal balance and kisspeptin 40 nmol/rat daily S/C injection show effect in normal restoring of female reproductive hormonal and histological balance of PCOS rats.","PeriodicalId":17869,"journal":{"name":"Kufa Journal For Veterinary Medical Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73977401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-06-30DOI: 10.36326/kjvs/2020/v11i13300
M. J. Al-Kurdy, K. Khudair
The current study was aimed to explore the effect of black currant selenium nanoparticles (BCSeNPs)on serum lipid profile and oxidant- antioxidant state markers in blood of D- galactose(D-gal) treated rats. The green synthesis of SeNPs as described earlier in our previous study was characterized by color changes; Ultraviolet- visible (UV–VIS) spectroscopy; scanning electron microscopy (SEM) techniques; X–ray diffraction analysis (XRD); Fourier transform infrared spectroscopy (FTIR). The results revealed prevalence of brick- red color of BCSeNPs characterized by spherical crystals with average particle size in the range of 18-50 nm. Thirty-two (32) adult male rats were divided randomly and equally into four experimental groups (8/group) and handles as follows for eight weeks: Control (C) group: rats in this group were treated with intra peritoneal injection (i.p) and oral intubation of normal saline. T1 group: animals in this group were subjected to i.p. of D gal a dose (150 mg/kg/day), which was dissolved in normal saline solution. T2 group: the rats were oral intubation BCSeNPs (1mg/Kg. B. W). T3 group: rats in this group were administered BCSeNPs concurrently with i.p. of D-gal in the same previous doses. Blood samples were collected from heart by cardio puncture technique at 2nd and 8th weeks of the experiment and serum samples were used for estimation of some biochemical parameters related to oxidant-antioxidant status markers: Malondialdehyde (MDA) and Total antioxidant capacity (TAO-C); Serum lipid profile: concentration of total cholesterol(TC), triglyceride(TAG), high density lipoprotein- cholesterol(HDL-c), low density lipoprotein- cholesterol (LDL-c), and very low density lipoprotein- cholesterol(VLDL-c). At the end of experiment, after animal scarifying, section from liver was taken for detection of gene expression of glutathione peroxidase. The results of here in study confirmed a case the oxidative stress and hyperlipidemia in T1 group manifested by significant depression in serum TAO-C concentration and decrease in glutathione peroxidase gene expression level. While, caused elevation in serum MDA concentration and significant decrease in serum concentration of HDL-c with significant elevation in serum concentration of TAG, VLDL-C, TC, and LDL-c. At the end of experiment, BCSeNPs intubation in T2 group caused alleviation of previous mentioned parameters related to oxidative stress and lipid profile. On conclusion, the result in a current study showed that black currant selenium nanoparticle has both a preventive and a therapeutic role in D-gal toxicity in adult male rats, where BCSeNPs can be considered as antioxidant and hypolipidemic agent.
本研究旨在探讨黑加仑硒纳米颗粒(BCSeNPs)对D-半乳糖(D-gal)处理大鼠血脂和血液中氧化-抗氧化状态指标的影响。在我们之前的研究中描述的绿色合成SeNPs的特征是颜色变化;紫外-可见光谱学;扫描电镜(SEM)技术;x射线衍射分析(XRD);傅立叶变换红外光谱(FTIR)。结果表明，BCSeNPs普遍呈砖红色，其平均粒径在18 ~ 50 nm之间。将32只成年雄性大鼠随机平均分为4个实验组(8只/组)，按以下方法处理8周:对照组(C)组:给予腹腔注射(i.p)和生理盐水口腔插管治疗。T1组:该组动物ig 150 mg/kg/day D gal a剂量，溶解于生理盐水中。T2组:大鼠口服BCSeNPs (1mg/Kg);B. W . T3组:该组大鼠与D-gal同时给予相同剂量的BCSeNPs。实验第2周和第8周采用穿刺法采集心脏血样，测定血清中与氧化-抗氧化状态标志物相关的生化参数:丙二醛(MDA)和总抗氧化能力(TAO-C);血脂谱:总胆固醇(TC)、甘油三酯(TAG)、高密度脂蛋白-胆固醇(HDL-c)、低密度脂蛋白-胆固醇(LDL-c)和极低密度脂蛋白-胆固醇(VLDL-c)的浓度。实验结束，动物瘢痕化后，取肝脏切片检测谷胱甘肽过氧化物酶基因表达。本研究结果证实了T1组氧化应激及高脂血症的一例，表现为血清中TAO-C浓度明显降低，谷胱甘肽过氧化物酶基因表达水平降低。引起血清MDA浓度升高，血清HDL-c浓度显著降低，血清TAG、VLDL-C、TC、LDL-c浓度显著升高。实验结束时，T2组插管BCSeNPs后，上述氧化应激及血脂相关参数均有所缓解。综上所述，目前的一项研究结果表明，黑加仑硒纳米颗粒对成年雄性大鼠d -半乳糖毒性既有预防作用，也有治疗作用，其中BCSeNPs可以被认为是抗氧化剂和降血脂剂。
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