Pub Date : 2024-08-30DOI: 10.1038/s41684-024-01438-8
Jorge Ferreira
{"title":"Kaempferol-3-rhamnoside can help treat diabetes in mice","authors":"Jorge Ferreira","doi":"10.1038/s41684-024-01438-8","DOIUrl":"10.1038/s41684-024-01438-8","url":null,"abstract":"","PeriodicalId":17936,"journal":{"name":"Lab Animal","volume":"53 9","pages":"223-223"},"PeriodicalIF":5.9,"publicationDate":"2024-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.nature.com/articles/s41684-024-01438-8.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142108835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-08-30DOI: 10.1038/s41684-024-01436-w
Alexandra Le Bras
{"title":"Effect of housing temperature on drug-induced weight loss","authors":"Alexandra Le Bras","doi":"10.1038/s41684-024-01436-w","DOIUrl":"10.1038/s41684-024-01436-w","url":null,"abstract":"","PeriodicalId":17936,"journal":{"name":"Lab Animal","volume":"53 9","pages":"221-221"},"PeriodicalIF":5.9,"publicationDate":"2024-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.nature.com/articles/s41684-024-01436-w.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142108834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-08-30DOI: 10.1038/s41684-024-01431-1
Alexandra Le Bras
{"title":"Metformin treatment for fragile X syndrome","authors":"Alexandra Le Bras","doi":"10.1038/s41684-024-01431-1","DOIUrl":"10.1038/s41684-024-01431-1","url":null,"abstract":"","PeriodicalId":17936,"journal":{"name":"Lab Animal","volume":"53 9","pages":"218-218"},"PeriodicalIF":5.9,"publicationDate":"2024-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142101881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nonhuman primates (NHPs), which are closely related to humans, are useful in biomedical research, and an increasing number of NHP disease models have been reported using gene editing. However, many disease-related genes cause perinatal death when manipulated homozygously by gene editing. In addition, NHP resources, which are limited, should be efficiently used. Here, to address these issues, we developed a method of introducing heterozygous genetic modifications into common marmosets by combining Platinum transcription activator-like effector nuclease (TALEN) and a gene-editing strategy in oocytes. We succeeded in introducing the heterozygous exon 9 deletion mutation in the presenilin 1 gene, which causes familial Alzheimer’s disease in humans, using this technology. As a result, we obtained animals with the expected genotypes and confirmed several Alzheimer’s disease-related biochemical changes. This study suggests that highly efficient heterozygosity-oriented gene editing is possible using TALEN and oocytes and is an effective method for producing genetically modified animals. This study shows that using gene-editing technology in oocytes using Platinum TALEN is an effective method for producing genetically modified marmosets by exon skipping.
{"title":"Production of a heterozygous exon skipping model of common marmosets using gene-editing technology","authors":"Kenya Sato, Hiroki Sasaguri, Wakako Kumita, Tetsushi Sakuma, Tomoe Morioka, Kenichi Nagata, Takashi Inoue, Yoko Kurotaki, Naomi Mihira, Michihira Tagami, Ri-ichiroh Manabe, Kokoro Ozaki, Yasushi Okazaki, Takashi Yamamoto, Makoto Suematsu, Takaomi C. Saido, Erika Sasaki","doi":"10.1038/s41684-024-01424-0","DOIUrl":"10.1038/s41684-024-01424-0","url":null,"abstract":"Nonhuman primates (NHPs), which are closely related to humans, are useful in biomedical research, and an increasing number of NHP disease models have been reported using gene editing. However, many disease-related genes cause perinatal death when manipulated homozygously by gene editing. In addition, NHP resources, which are limited, should be efficiently used. Here, to address these issues, we developed a method of introducing heterozygous genetic modifications into common marmosets by combining Platinum transcription activator-like effector nuclease (TALEN) and a gene-editing strategy in oocytes. We succeeded in introducing the heterozygous exon 9 deletion mutation in the presenilin 1 gene, which causes familial Alzheimer’s disease in humans, using this technology. As a result, we obtained animals with the expected genotypes and confirmed several Alzheimer’s disease-related biochemical changes. This study suggests that highly efficient heterozygosity-oriented gene editing is possible using TALEN and oocytes and is an effective method for producing genetically modified animals. This study shows that using gene-editing technology in oocytes using Platinum TALEN is an effective method for producing genetically modified marmosets by exon skipping.","PeriodicalId":17936,"journal":{"name":"Lab Animal","volume":"53 9","pages":"244-251"},"PeriodicalIF":5.9,"publicationDate":"2024-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.nature.com/articles/s41684-024-01424-0.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142100827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-08-30DOI: 10.1038/s41684-024-01430-2
Alexandra Le Bras
{"title":"Sex differences in microbiota","authors":"Alexandra Le Bras","doi":"10.1038/s41684-024-01430-2","DOIUrl":"10.1038/s41684-024-01430-2","url":null,"abstract":"","PeriodicalId":17936,"journal":{"name":"Lab Animal","volume":"53 9","pages":"218-218"},"PeriodicalIF":5.9,"publicationDate":"2024-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142100822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sudden unexpected death in epilepsy (SUDEP) is the leading cause of death among patients with epilepsy, causing a global public health burden. The underlying mechanisms of SUDEP remain elusive, and effective prevention or treatment strategies require further investigation. A major challenge in current SUDEP research is the lack of an ideal model that maximally mimics the human condition. Animal models are important for revealing the potential pathogenesis of SUDEP and preventing its occurrence; however, they have potential limitations due to species differences that prevent them from precisely replicating the intricate physiological and pathological processes of human disease. This Review provides a comprehensive overview of several available SUDEP animal models, highlighting their pros and cons. More importantly, we further propose the establishment of an ideal model based on brain–computer interfaces and artificial intelligence, hoping to offer new insights into potential advancements in SUDEP research. In doing so, we hope to provide valuable information for SUDEP researchers, offer new insights into the pathogenesis of SUDEP and open new avenues for the development of strategies to prevent SUDEP. This Review provides a comprehensive overview of several available animal models of sudden unexpected death in epilepsy (SUDEP), highlighting their pros and cons to elucidate SUDEP causes and mechanisms.
{"title":"Challenges and future directions of SUDEP models","authors":"JiaXuan Gu, WeiHui Shao, Lu Liu, YuLing Wang, Yue Yang, ZhuoYue Zhang, YaXuan Wu, Qing Xu, LeYuan Gu, YuanLi Zhang, Yue Shen, HaiTing Zhao, Chang Zeng, HongHai Zhang","doi":"10.1038/s41684-024-01426-y","DOIUrl":"10.1038/s41684-024-01426-y","url":null,"abstract":"Sudden unexpected death in epilepsy (SUDEP) is the leading cause of death among patients with epilepsy, causing a global public health burden. The underlying mechanisms of SUDEP remain elusive, and effective prevention or treatment strategies require further investigation. A major challenge in current SUDEP research is the lack of an ideal model that maximally mimics the human condition. Animal models are important for revealing the potential pathogenesis of SUDEP and preventing its occurrence; however, they have potential limitations due to species differences that prevent them from precisely replicating the intricate physiological and pathological processes of human disease. This Review provides a comprehensive overview of several available SUDEP animal models, highlighting their pros and cons. More importantly, we further propose the establishment of an ideal model based on brain–computer interfaces and artificial intelligence, hoping to offer new insights into potential advancements in SUDEP research. In doing so, we hope to provide valuable information for SUDEP researchers, offer new insights into the pathogenesis of SUDEP and open new avenues for the development of strategies to prevent SUDEP. This Review provides a comprehensive overview of several available animal models of sudden unexpected death in epilepsy (SUDEP), highlighting their pros and cons to elucidate SUDEP causes and mechanisms.","PeriodicalId":17936,"journal":{"name":"Lab Animal","volume":"53 9","pages":"226-243"},"PeriodicalIF":5.9,"publicationDate":"2024-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142073205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-08-19DOI: 10.1038/s41684-024-01427-x
Alexandra Le Bras
The Laboratory Animal Genetic Reporting (LAG-R) framework, developed by a team of international experts, was recently published in Nature Communications. The LAG-R framework proposes a collection of guidelines to improve the reporting of genetic information for animal models. Lab Animal spoke with corresponding authors Lydia Teboul and Guillaume Pavlovic to know more about the motivation for these recommendations, the benefits for researchers and how to accelerate their adoption.
{"title":"A closer look at the Laboratory Animal Genetic Reporting (LAG-R) guidelines","authors":"Alexandra Le Bras","doi":"10.1038/s41684-024-01427-x","DOIUrl":"10.1038/s41684-024-01427-x","url":null,"abstract":"The Laboratory Animal Genetic Reporting (LAG-R) framework, developed by a team of international experts, was recently published in Nature Communications. The LAG-R framework proposes a collection of guidelines to improve the reporting of genetic information for animal models. Lab Animal spoke with corresponding authors Lydia Teboul and Guillaume Pavlovic to know more about the motivation for these recommendations, the benefits for researchers and how to accelerate their adoption.","PeriodicalId":17936,"journal":{"name":"Lab Animal","volume":"53 9","pages":"215-216"},"PeriodicalIF":5.9,"publicationDate":"2024-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142002715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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