Pub Date : 2024-08-30DOI: 10.1038/s41684-024-01430-2
Alexandra Le Bras
{"title":"Sex differences in microbiota","authors":"Alexandra Le Bras","doi":"10.1038/s41684-024-01430-2","DOIUrl":"10.1038/s41684-024-01430-2","url":null,"abstract":"","PeriodicalId":17936,"journal":{"name":"Lab Animal","volume":"53 9","pages":"218-218"},"PeriodicalIF":5.9,"publicationDate":"2024-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142100822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sudden unexpected death in epilepsy (SUDEP) is the leading cause of death among patients with epilepsy, causing a global public health burden. The underlying mechanisms of SUDEP remain elusive, and effective prevention or treatment strategies require further investigation. A major challenge in current SUDEP research is the lack of an ideal model that maximally mimics the human condition. Animal models are important for revealing the potential pathogenesis of SUDEP and preventing its occurrence; however, they have potential limitations due to species differences that prevent them from precisely replicating the intricate physiological and pathological processes of human disease. This Review provides a comprehensive overview of several available SUDEP animal models, highlighting their pros and cons. More importantly, we further propose the establishment of an ideal model based on brain–computer interfaces and artificial intelligence, hoping to offer new insights into potential advancements in SUDEP research. In doing so, we hope to provide valuable information for SUDEP researchers, offer new insights into the pathogenesis of SUDEP and open new avenues for the development of strategies to prevent SUDEP. This Review provides a comprehensive overview of several available animal models of sudden unexpected death in epilepsy (SUDEP), highlighting their pros and cons to elucidate SUDEP causes and mechanisms.
{"title":"Challenges and future directions of SUDEP models","authors":"JiaXuan Gu, WeiHui Shao, Lu Liu, YuLing Wang, Yue Yang, ZhuoYue Zhang, YaXuan Wu, Qing Xu, LeYuan Gu, YuanLi Zhang, Yue Shen, HaiTing Zhao, Chang Zeng, HongHai Zhang","doi":"10.1038/s41684-024-01426-y","DOIUrl":"10.1038/s41684-024-01426-y","url":null,"abstract":"Sudden unexpected death in epilepsy (SUDEP) is the leading cause of death among patients with epilepsy, causing a global public health burden. The underlying mechanisms of SUDEP remain elusive, and effective prevention or treatment strategies require further investigation. A major challenge in current SUDEP research is the lack of an ideal model that maximally mimics the human condition. Animal models are important for revealing the potential pathogenesis of SUDEP and preventing its occurrence; however, they have potential limitations due to species differences that prevent them from precisely replicating the intricate physiological and pathological processes of human disease. This Review provides a comprehensive overview of several available SUDEP animal models, highlighting their pros and cons. More importantly, we further propose the establishment of an ideal model based on brain–computer interfaces and artificial intelligence, hoping to offer new insights into potential advancements in SUDEP research. In doing so, we hope to provide valuable information for SUDEP researchers, offer new insights into the pathogenesis of SUDEP and open new avenues for the development of strategies to prevent SUDEP. This Review provides a comprehensive overview of several available animal models of sudden unexpected death in epilepsy (SUDEP), highlighting their pros and cons to elucidate SUDEP causes and mechanisms.","PeriodicalId":17936,"journal":{"name":"Lab Animal","volume":"53 9","pages":"226-243"},"PeriodicalIF":5.9,"publicationDate":"2024-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142073205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-08-19DOI: 10.1038/s41684-024-01427-x
Alexandra Le Bras
The Laboratory Animal Genetic Reporting (LAG-R) framework, developed by a team of international experts, was recently published in Nature Communications. The LAG-R framework proposes a collection of guidelines to improve the reporting of genetic information for animal models. Lab Animal spoke with corresponding authors Lydia Teboul and Guillaume Pavlovic to know more about the motivation for these recommendations, the benefits for researchers and how to accelerate their adoption.
{"title":"A closer look at the Laboratory Animal Genetic Reporting (LAG-R) guidelines","authors":"Alexandra Le Bras","doi":"10.1038/s41684-024-01427-x","DOIUrl":"10.1038/s41684-024-01427-x","url":null,"abstract":"The Laboratory Animal Genetic Reporting (LAG-R) framework, developed by a team of international experts, was recently published in Nature Communications. The LAG-R framework proposes a collection of guidelines to improve the reporting of genetic information for animal models. Lab Animal spoke with corresponding authors Lydia Teboul and Guillaume Pavlovic to know more about the motivation for these recommendations, the benefits for researchers and how to accelerate their adoption.","PeriodicalId":17936,"journal":{"name":"Lab Animal","volume":"53 9","pages":"215-216"},"PeriodicalIF":5.9,"publicationDate":"2024-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142002715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-08-14DOI: 10.1038/s41684-024-01425-z
Fabien Filaire, Sander Herfst
A highly pathogenic H5N1 influenza virus is spreading in U.S. dairy cattle and has been transmitted to other species, including humans, probably through contaminated milk. Understanding how the virus spreads among cattle and its potential for mammalian adaptation and airborne transmission is crucial for effective outbreak control and public health safety.
{"title":"The highly pathogenic H5N1 virus found in U.S. dairy cattle has some characteristics that could enhance infection and transmission among mammals","authors":"Fabien Filaire, Sander Herfst","doi":"10.1038/s41684-024-01425-z","DOIUrl":"10.1038/s41684-024-01425-z","url":null,"abstract":"A highly pathogenic H5N1 influenza virus is spreading in U.S. dairy cattle and has been transmitted to other species, including humans, probably through contaminated milk. Understanding how the virus spreads among cattle and its potential for mammalian adaptation and airborne transmission is crucial for effective outbreak control and public health safety.","PeriodicalId":17936,"journal":{"name":"Lab Animal","volume":"53 9","pages":"224-225"},"PeriodicalIF":5.9,"publicationDate":"2024-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.nature.com/articles/s41684-024-01425-z.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141980956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-08-09DOI: 10.1038/s41684-024-01410-6
Daniel G. E. Thiem, Daniel Stephan, Alexander Ziebart, Robert Ruemmler, Julian Riedel, Shankeeth Vinayahalingam, Bilal Al-Nawas, Sebastian Blatt, Peer W. Kämmerer
Free flap failure represents a substantial clinical burden. The role of intraoperative volume management remains controversial, with valid studies lacking. Here, using a large animal model, we investigated the influence of volume management on free flap perfusion and metabolism. Autotransfer of a musculocutaneous gracilis flap was performed on 31 German domestic pigs, with arterial anastomosis and catheterization of the pedicle vein for sequential blood sampling. Flap reperfusion was followed by induction of a hemorrhagic shock with maintenance for 30 min and subsequent circulation stabilization with crystalloid solution, crystalloid solution and catecholamine, autotransfusion or colloidal solution. Flap perfusion and oxygenation were periodically assessed using hyperspectral imaging. Flap metabolism was assessed via periodic blood gas analyses. Hyperspectral imaging revealed no difference in either superficial or deep tissue oxygen saturation, tissue hemoglobin or tissue water content between the test groups at any time point. Blood gas analyses showed that lactate levels were significantly increased in the group that received crystalloid solution and catecholamine, after circulatory stabilization and up to 2 h after. We conclude that, in hemorrhagic shock, volume management impacts acid–base balance in free flaps. Crystalloid solutions with norepinephrine increase lactate levels, yet short-term effects on flap perfusion seem minimal, suggesting that vasopressors are not detrimental. Free flap is widely used in head and neck reconstructive surgery, but fluid management of surgical patients remains controversial. Here, Thiem et al. used a pig model to monitor the effects of volume management on free flap perfusion and metabolism.
{"title":"Effects of volume management on free flap perfusion and metabolism in a large animal model study","authors":"Daniel G. E. Thiem, Daniel Stephan, Alexander Ziebart, Robert Ruemmler, Julian Riedel, Shankeeth Vinayahalingam, Bilal Al-Nawas, Sebastian Blatt, Peer W. Kämmerer","doi":"10.1038/s41684-024-01410-6","DOIUrl":"10.1038/s41684-024-01410-6","url":null,"abstract":"Free flap failure represents a substantial clinical burden. The role of intraoperative volume management remains controversial, with valid studies lacking. Here, using a large animal model, we investigated the influence of volume management on free flap perfusion and metabolism. Autotransfer of a musculocutaneous gracilis flap was performed on 31 German domestic pigs, with arterial anastomosis and catheterization of the pedicle vein for sequential blood sampling. Flap reperfusion was followed by induction of a hemorrhagic shock with maintenance for 30 min and subsequent circulation stabilization with crystalloid solution, crystalloid solution and catecholamine, autotransfusion or colloidal solution. Flap perfusion and oxygenation were periodically assessed using hyperspectral imaging. Flap metabolism was assessed via periodic blood gas analyses. Hyperspectral imaging revealed no difference in either superficial or deep tissue oxygen saturation, tissue hemoglobin or tissue water content between the test groups at any time point. Blood gas analyses showed that lactate levels were significantly increased in the group that received crystalloid solution and catecholamine, after circulatory stabilization and up to 2 h after. We conclude that, in hemorrhagic shock, volume management impacts acid–base balance in free flaps. Crystalloid solutions with norepinephrine increase lactate levels, yet short-term effects on flap perfusion seem minimal, suggesting that vasopressors are not detrimental. Free flap is widely used in head and neck reconstructive surgery, but fluid management of surgical patients remains controversial. Here, Thiem et al. used a pig model to monitor the effects of volume management on free flap perfusion and metabolism.","PeriodicalId":17936,"journal":{"name":"Lab Animal","volume":"53 10","pages":"268-275"},"PeriodicalIF":5.9,"publicationDate":"2024-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.nature.com/articles/s41684-024-01410-6.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141908977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-08-01DOI: 10.1038/s41684-024-01418-y
Alexandra Le Bras
{"title":"Humidity and heat cause anxiety in mice","authors":"Alexandra Le Bras","doi":"10.1038/s41684-024-01418-y","DOIUrl":"10.1038/s41684-024-01418-y","url":null,"abstract":"","PeriodicalId":17936,"journal":{"name":"Lab Animal","volume":"53 8","pages":"192-192"},"PeriodicalIF":5.9,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141875245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-08-01DOI: 10.1038/s41684-024-01419-x
Alexandra Le Bras
{"title":"New guidelines for rodent cancer models","authors":"Alexandra Le Bras","doi":"10.1038/s41684-024-01419-x","DOIUrl":"10.1038/s41684-024-01419-x","url":null,"abstract":"","PeriodicalId":17936,"journal":{"name":"Lab Animal","volume":"53 8","pages":"192-192"},"PeriodicalIF":5.9,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141875249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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