Pub Date : 2025-01-03DOI: 10.1038/s41684-024-01501-4
Alexandra Le Bras
{"title":"Impact of muscle clock on lifespan","authors":"Alexandra Le Bras","doi":"10.1038/s41684-024-01501-4","DOIUrl":"10.1038/s41684-024-01501-4","url":null,"abstract":"","PeriodicalId":17936,"journal":{"name":"Lab Animal","volume":"54 1","pages":"4-4"},"PeriodicalIF":5.9,"publicationDate":"2025-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.nature.com/articles/s41684-024-01501-4.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142925790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-03DOI: 10.1038/s41684-024-01502-3
Alexandra Le Bras
{"title":"Linking social stress, senescence and aging","authors":"Alexandra Le Bras","doi":"10.1038/s41684-024-01502-3","DOIUrl":"10.1038/s41684-024-01502-3","url":null,"abstract":"","PeriodicalId":17936,"journal":{"name":"Lab Animal","volume":"54 1","pages":"5-5"},"PeriodicalIF":5.9,"publicationDate":"2025-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.nature.com/articles/s41684-024-01502-3.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142925783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-03DOI: 10.1038/s41684-024-01493-1
Alexandra Le Bras
{"title":"Role of mitochondrial DNA in fly immunity","authors":"Alexandra Le Bras","doi":"10.1038/s41684-024-01493-1","DOIUrl":"10.1038/s41684-024-01493-1","url":null,"abstract":"","PeriodicalId":17936,"journal":{"name":"Lab Animal","volume":"54 1","pages":"1-1"},"PeriodicalIF":5.9,"publicationDate":"2025-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142916908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-12-05DOI: 10.1038/s41684-024-01477-1
Megan M. Knuth, Carolina Vieira Campos, Kirsten Smith, Elizabeth K. Hutchins, Shantae Lewis, Mary York, Lyndon M. Coghill, Craig Franklin, Amanda J. MacFarlane, Aaron C. Ericsson, Terry Magnuson, Folami Ideraabdullah
Standard chow diets influence reproducibility in animal model experiments because chows have different nutrient compositions, which can independently influence phenotypes. However, there is little evidence of the role of timing in the extent of variability caused by chow exposure. Here we measured the impact of different diets (5V5M, 5V0G, 2920X and 5058) and timing of exposure (adult exposure (AE), lifetime exposure (LE) and developmental exposure (DE)) on growth and development, metabolic health indicators and gut bacterial microbiota profiles across genetically identical C57BL/6J mice. Diet drove differences in macro- and micronutrient intake for all exposure models. AE had no effect on phenotypic outcomes. However, LE mice exhibited significant sex-dependent diet effects on growth, body weight and body composition. LE effects were mostly absent in the DE model, where mice were exposed to chow differences only from conception to weaning. Both AE and LE models exhibited similar diet-driven beta diversity profiles for the gut bacterial microbiota, with 5058 diet driving the most distinct profile. However, compared with AE, LE effects on beta diversity were sex dependent, and LE mice exhibited nine times more differentially abundant bacterial genera, the majority of which were inversely affected by 2920X and 5058 diets. Our findings demonstrate that LE to different chow diets has the greatest impact on the reproducibility of several experimental measures commonly used in preclinical mouse model studies. Importantly, weaning mice from different diets onto the same diet for maturation may be an effective way to reduce unwanted phenotypic variability among experimental models. Differences in standard chow diets can influence mouse model phenotypes and impact experimental reproducibility. Here Knuth et al. show that the timing during which mice are exposed to standard chow can also affect phenotypic outcomes.
{"title":"Timing of standard chow exposure determines the variability of mouse phenotypic outcomes and gut microbiota profile","authors":"Megan M. Knuth, Carolina Vieira Campos, Kirsten Smith, Elizabeth K. Hutchins, Shantae Lewis, Mary York, Lyndon M. Coghill, Craig Franklin, Amanda J. MacFarlane, Aaron C. Ericsson, Terry Magnuson, Folami Ideraabdullah","doi":"10.1038/s41684-024-01477-1","DOIUrl":"10.1038/s41684-024-01477-1","url":null,"abstract":"Standard chow diets influence reproducibility in animal model experiments because chows have different nutrient compositions, which can independently influence phenotypes. However, there is little evidence of the role of timing in the extent of variability caused by chow exposure. Here we measured the impact of different diets (5V5M, 5V0G, 2920X and 5058) and timing of exposure (adult exposure (AE), lifetime exposure (LE) and developmental exposure (DE)) on growth and development, metabolic health indicators and gut bacterial microbiota profiles across genetically identical C57BL/6J mice. Diet drove differences in macro- and micronutrient intake for all exposure models. AE had no effect on phenotypic outcomes. However, LE mice exhibited significant sex-dependent diet effects on growth, body weight and body composition. LE effects were mostly absent in the DE model, where mice were exposed to chow differences only from conception to weaning. Both AE and LE models exhibited similar diet-driven beta diversity profiles for the gut bacterial microbiota, with 5058 diet driving the most distinct profile. However, compared with AE, LE effects on beta diversity were sex dependent, and LE mice exhibited nine times more differentially abundant bacterial genera, the majority of which were inversely affected by 2920X and 5058 diets. Our findings demonstrate that LE to different chow diets has the greatest impact on the reproducibility of several experimental measures commonly used in preclinical mouse model studies. Importantly, weaning mice from different diets onto the same diet for maturation may be an effective way to reduce unwanted phenotypic variability among experimental models. Differences in standard chow diets can influence mouse model phenotypes and impact experimental reproducibility. Here Knuth et al. show that the timing during which mice are exposed to standard chow can also affect phenotypic outcomes.","PeriodicalId":17936,"journal":{"name":"Lab Animal","volume":"54 1","pages":"24-36"},"PeriodicalIF":5.9,"publicationDate":"2024-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142777002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-28DOI: 10.1038/s41684-024-01492-2
José Pedro Castro
A new study reveals that metformin, a widely prescribed diabetes medication, can significantly improve health parameters in aged male cynomolgus monkeys — possibly by slowing the aging process. The findings represent an important step toward understanding how pharmaceutical interventions might, in the future, extend the healthspan and delay age-related diseases in humans.
{"title":"Metformin and monkeys: what can we learn about delaying aging?","authors":"José Pedro Castro","doi":"10.1038/s41684-024-01492-2","DOIUrl":"10.1038/s41684-024-01492-2","url":null,"abstract":"A new study reveals that metformin, a widely prescribed diabetes medication, can significantly improve health parameters in aged male cynomolgus monkeys — possibly by slowing the aging process. The findings represent an important step toward understanding how pharmaceutical interventions might, in the future, extend the healthspan and delay age-related diseases in humans.","PeriodicalId":17936,"journal":{"name":"Lab Animal","volume":"54 1","pages":"7-8"},"PeriodicalIF":5.9,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142750980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-26DOI: 10.1038/s41684-024-01485-1
Alexandra Le Bras
{"title":"High-speed injection in C. elegans","authors":"Alexandra Le Bras","doi":"10.1038/s41684-024-01485-1","DOIUrl":"10.1038/s41684-024-01485-1","url":null,"abstract":"","PeriodicalId":17936,"journal":{"name":"Lab Animal","volume":"53 12","pages":"356-356"},"PeriodicalIF":5.9,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142713008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-26DOI: 10.1038/s41684-024-01491-3
Zhuoqing Fang, Gary Peltz
The laboratory mouse has been the premier model organism for biomedical research owing to the availability of multiple well-characterized inbred strains, its mammalian physiology and its homozygous genome, and because experiments can be performed under conditions that control environmental variables. Moreover, its genome can be genetically modified to assess the impact of allelic variation on phenotype. Mouse models have been used to discover or test many therapies that are commonly used today. Mouse genetic discoveries are often made using genome-wide association study methods that compare allelic differences in panels of inbred mouse strains with their phenotypic responses. Here we examine changes in the methods used to analyze mouse genetic models of biomedical traits during the twenty-first century. To do this, we first examine where mouse genetics was before the first inflection point, which was just before the revolution in genome sequencing that occurred 20 years ago, and then describe the factors that have accelerated the pace of mouse genetic discovery. We focus on mouse genetic studies that have generated findings that either were translated to humans or could impact clinical medicine or drug development. We next explore how advances in computational capabilities and in DNA sequencing methodology during the past 20 years could enhance the ability of mouse genetics to produce solutions for twenty-first century public-health problems. Mouse genetic studies are essential for our understanding of genotype–phenotype relationships. Here the authors discuss the technological advances that have accelerated the pace of mouse genetic discovery during the twenty-first century.
{"title":"Twenty-first century mouse genetics is again at an inflection point","authors":"Zhuoqing Fang, Gary Peltz","doi":"10.1038/s41684-024-01491-3","DOIUrl":"10.1038/s41684-024-01491-3","url":null,"abstract":"The laboratory mouse has been the premier model organism for biomedical research owing to the availability of multiple well-characterized inbred strains, its mammalian physiology and its homozygous genome, and because experiments can be performed under conditions that control environmental variables. Moreover, its genome can be genetically modified to assess the impact of allelic variation on phenotype. Mouse models have been used to discover or test many therapies that are commonly used today. Mouse genetic discoveries are often made using genome-wide association study methods that compare allelic differences in panels of inbred mouse strains with their phenotypic responses. Here we examine changes in the methods used to analyze mouse genetic models of biomedical traits during the twenty-first century. To do this, we first examine where mouse genetics was before the first inflection point, which was just before the revolution in genome sequencing that occurred 20 years ago, and then describe the factors that have accelerated the pace of mouse genetic discovery. We focus on mouse genetic studies that have generated findings that either were translated to humans or could impact clinical medicine or drug development. We next explore how advances in computational capabilities and in DNA sequencing methodology during the past 20 years could enhance the ability of mouse genetics to produce solutions for twenty-first century public-health problems. Mouse genetic studies are essential for our understanding of genotype–phenotype relationships. Here the authors discuss the technological advances that have accelerated the pace of mouse genetic discovery during the twenty-first century.","PeriodicalId":17936,"journal":{"name":"Lab Animal","volume":"54 1","pages":"9-15"},"PeriodicalIF":5.9,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.nature.com/articles/s41684-024-01491-3.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142712976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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