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Analysis of the informative value of the lysosomal cationic test (LCT) and of the kinetics of neutrophilic nuclear fragmentation helped reveal significant differences in the neutrophil granulocyte morphology and function in the patients with acute bacterial tonsillitis and acute respiratory viral infections. The detected varieties in A granule formation and in cellular ability to lysosomal secretion of cationic proteins allow the employment of LCT in the assessment of the disease severity and in the early differential diagnosis between tonsillitis and acute respiratory diseases.

The authors suggest a device for automated exposure in micro-photographing with the use of the Elektronika B5-22 flash light. This pulsed light source is optimal as regards its spectral composition. The gist of the method is as follows: the flash photographic element, built in a light-proof case, disposed on the microscope ocular, permits governing the light pulse length.

The authors have examined the possibility of using sectional polystyrene plates, made in this country, in time-resolved fluoroimmunoassay (tr-FIA) with Venezuelan equine encephalomyelitis and tick-borne encephalitis arboviruses, and with influenza A virus. The plates presensitized with specific antibodies were found fit for the detection of the antigens of the above viruses. These plates are not recommended for the detection of influenza A virus-specific proteins adsorbed directly onto the microplate surface.

Glycogen and protein concentrations and the activities of liver glycogen metabolic enzymes were measured in 22 children aged 4 to 15, suffering from extrahepatic portal hypertension. Glucose-6-phosphatase, amylo-1,6-glucosidase, fructose-1,6-diphosphatase, phosphorylases alpha and beta, phosphoglucomutase, and phosphohexose isomerase levels were analyzed. Liver biopsy specimens obtained by surgical marginal biopsy were used in the study. No or drastic reduction of phosphorylase alpha activity and reduction of glycogen concentration and glucose-phosphatase activity were found characteristic of extrahepatic hypertension. Analysis of correlations of the findings has demonstrated a medium correlation in 4 cases and a strong correlation between the findings in 1 case, the possibility being estimated as 0.95-0.99. The highest number of correlations was observed with phosphorylase alpha and glucose-6-phosphatase (3 correlations). Liver blood stream impairments result in injury to one of its main biochemical functions, i.e., the maintenance of blood glucose homeostasis, this leading to reduction of the adaptation potential of the body; this should be borne in mind when planning therapeutic measures for patients with extrahepatic hypertension.

Elevated spontaneous liberation of biologically active substances from the peripheral blood leukocytes was revealed in 42% of children suffering from various allergic conditions. This liberation was particularly marked during the disease exacerbation and in grave forms of allergies in the patients with elevated serum IgG levels and in those with bronchial hyperreactivity. Liberation of biologically active substances was more often recorded in schoolchildren than in preschool children. Membrane stabilizers (intal and zaditen) were more effective in the patients with elevated liberation of biologically active substances. The authors have analyzed the conditions of tests for spontaneous liberation of biologically active substances and some characteristics of these biologically active substances.

Antibacterial sera activities towards Citrobacter O22 and Salmonella typhimurium were tested with O22, O1, O4, O5, O12 erythrocytic diagnostic agents. Cross activities of O22, O1, O4, O5, O12, and Hi antigens were tested with the same and Hi erythrocytic diagnostic agents in the antibody neutralization test. The findings have confirmed the identity or very close relationship between the tested O antigens. Screening for antigens of the excretions from patients with S. typhimurium and Citrobacter O22 infections has shown that indication of Hi antigen may be considered as the differentiating criterion between these infections.