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Gas chromatographic measurement of fatty acid spectrum and free cholesterol level in exhaled air condensate of children suffering from asthma has revealed a deficiency of polyunsaturated fatty acids and lipids at the expense of linoleic and arachidonic acids.

Examinations of 33 patients with hypertrophic cardiomyopathy have revealed Stages I-II circulation insufficiency in 15. The findings were compared with those in 20 untrained normal subjects and 8 athletes. Lysosomal cationic protein levels were measured in polymorphonuclear leukocytes by the cytochemical method at rest, at the peak of dosed exercise (permanently increasing bicycle spiroergometry) and over the course of the recovery period--in 3, 12, and 24 h after exercise. The neutrophil distribution was also estimated by the staining intensity. Lysosomal cationic protein levels in hypertrophic cardiomyopathy patients with and without circulation insufficiency were changed to a different degree; these characteristics essentially varied in the patients, normal untrained subjects, and athletes. Changed levels of lysosomal cationic proteins appear to reflect the microcirculation disorders and the augmenting hypoxemia in the patients with circulation insufficiency, and thus may be used as a criterion to assess the clinical course of hypertrophic cardiomyopathy.

The suggested method for Willebrand's factor detection differs from the known methods by a 2-3 times higher sensitivity and takes 10 times less blood. The technique is based on estimation of washed formalin-treated donor platelet ristomycin aggregation index by scintillating these cells in Laborskel automated scintillator in supernatants of 2 samples with 0.1% ristomycin solution after the tested platelet-free plasma is added to one of these samples. Willebrand's factor level is found on the calibration curve of standard dilutions of a mixture of platelet-free donor plasma. The method is sufficiently simple, operative, and sensitive; it permits the diagnosis of not only Willebrand's disease, but of the hyperaggregation syndromes associated with vascular endothelium involvement that occur in various conditions, as well; it helps monitoring the efficacy of substitute and antiaggregation therapy.

Various methods for sheep red cell sensitization for the preparation of erythrocytic diagnostic agent from staphylococcal proteinase were under study. Sensitization with chromium chloride yielded the best results as regarded the sensitivity of the agent, optimal sensitizing dose, and sensitin consumption. The resultant erythrocytic diagnostic agent retained about 10% of the initial enzymic solution activity. The authors have demonstrated a high specificity of the erythrocytic diagnostic agent from staphylococcal proteinase, that may be used for the serologic diagnosis of staphylococcal infection and for proteinase indication in various staphylococcal preparations.

Amniotic fluid glucose levels were measured in 125 pregnant women included in high-risk group in respect of perinatal pathology. In the reference group the measurements have demonstrated an agreement between glucose concentrations in the amniotic fluid and gestation term and an inverse relationship between pregnancy progress and glucose level, whereas in diabetics this relationship was direct. Fetal developmental defects were associated with elevated glucose content in the amniotic fluid, concomitant placental abnormalities were conducive to reduction of these levels, and no correlations between this parameter and pregnancy terms were observed.

A sensitive bioluminescent rapid method for estimating the activity of pyridine nucleotide-(phosphate)-dependent glutamate dehydrogenases (NAD(P)-GDH) of human peripheral blood lymphocytes is suggested. 10000 cells is sufficient per analysis, up to 100 measurements may be made within 3 hrs. Normal subjects and subjects often suffering from acute respiratory diseases were examined for the aforesaid parameter. The findings evidence the diagnostic significance of NAD(P)-GDH for the detection of subjects at risk of developing acute respiratory diseases.

Heparin elevates the level of NBT-positive monocytes in donors and patients with osteoarthrosis deformans and rheumatoid arthritis. Patient's rheumatoid arthritis cells were found the most sensitive to heparin. Excessive heparin-dependent activity of monocytes in this case reflects the activity of the rheumatoid process, the NBT test with heparin being more sensitive in the diagnosis of disease activity than measurement of red cell sedimentation rate or of blood C-reactive protein, gamma- and alpha 2-globulin levels.

Examinations of Pseudomonas aeruginosa strains isolated from clinical material from newborns and puerperae and from the environmental objects (washings off, water) have shown that 45.45% of these strains belonged to serotype 04, 24.55% to serotype 02, and 16.38% to other serotypes. Serotype 04 was the most incident in all the tested samples, it was detectable in newborns' biotopes and on environmental objects. Serotype 02 strains ranked second in incidence; they were detected in clinical material from the newborns but not on environmental objects.

A simple and sensitive method for rapid detection of an antigen is offered. 0.025 ml of antiserum is placed on the slide, 0.025 ml of clarified extract containing the antigen added, and then 0.025 ml of intact 20.10(6) suspension of Kowan 1 Staphylococcus strain is added. When antigen-antibody complex is forming, staphylococcal cell aggregation starts. A staphylococcal reagent, manufactured by the Pasteur Research Institute of Epidemiology and Microbiology in Leningrad, may be employed, this making the method more available. The indication sensitivity is 0.2-1.25 mln cells per ml, this value depending on the bacterial species.