Hydrophilic endogenous bile acids ursodeoxycholic acid (UDCA), tauroursodeoxycholic acid (TUDCA), and glucourosodeoxycholic acid (GUDCA) have suggested neuroprotective effects. We performed a case–control study to examine the association between ALS diagnosis and serum levels of bile acids. Sporadic and familial ALS patients, age- and sex-matched healthy controls, and presymptomatic gene carriers who donated blood samples were included. Non-fasted serum samples stored at −80°C were used for the analysis. Serum bile acid levels were measured by liquid chromatography-mass spectrometry (LC–MS). Concentrations of 15 bile acids were obtained, 5 non-conjugated and 10 conjugated, and compared between ALS versus control groups (presymptomatic gene carriers + healthy controls) using the Wilcoxon-Rank-Sum test. In total, 80 participants were included: 31 ALS (17 sporadic and 14 familial ALS); 49 controls (22 gene carriers, 27 healthy controls). The mean age was 50 years old and 50% were male. In the ALS group, 45% had familial disease with a pathogenic variant in C9orf72 (29%), TARDBP (10%), FUS (3%), and CHCHD10 (3%) genes. In the control group, 43% carried pathogenic variants: C9orf72 (27%), SOD1 (10%), and FUS (6%). The serum levels of UDCA, TUDCA, and GUDCA trended higher in the ALS group compared to controls (median 27 vs. 7 nM, 4 vs. 3 nM, 110 vs. 47 nM, p-values 0.04, 0.06, 0.04, respectively). No significant group differences were found in other bile acids serum levels. In conclusion, the serum level of UDCA, TUDCA, GUDCA trended higher in ALS patients compared to controls, and no evidence of deficiencies was found.
亲水性内源性胆汁酸熊去氧胆酸(UDCA)、牛磺酸去氧胆酸(TUDCA)和葡萄糖去氧胆酸(GUDCA)具有神经保护作用。我们进行了一项病例对照研究,以探讨 ALS 诊断与血清胆汁酸水平之间的关系。研究对象包括散发性和家族性 ALS 患者、年龄和性别匹配的健康对照者以及捐献血样的无症状基因携带者。分析使用的是储存在 -80°C 的非空腹血清样本。血清胆汁酸水平通过液相色谱-质谱法(LC-MS)进行测定。采用 Wilcoxon-Rank-Sum 检验法比较 ALS 组和对照组(无症状基因携带者 + 健康对照组)之间的差异。共纳入 80 名参与者:31名 ALS 患者(17 名散发性 ALS 患者和 14 名家族性 ALS 患者);49 名对照组患者(22 名基因携带者和 27 名健康对照组患者)。平均年龄为 50 岁,50% 为男性。在肌萎缩性脊髓侧索硬化症组中,45%的人患有家族性疾病,其致病变异基因包括C9orf72(29%)、TARDBP(10%)、FUS(3%)和CHCHD10(3%)。在对照组中,43%携带致病变体:C9orf72(27%)、SOD1(10%)和 FUS(6%)。与对照组相比,ALS 组血清中的 UDCA、TUDCA 和 GUDCA 水平呈上升趋势(中位数分别为 27 nM 对 7 nM、4 nM 对 3 nM、110 nM 对 47 nM,P 值分别为 0.04、0.06、0.04)。其他胆汁酸血清水平没有发现明显的组间差异。总之,与对照组相比,ALS 患者血清中的 UDCA、TUDCA、GUDCA 水平呈上升趋势,没有发现缺乏的证据。
{"title":"Alteration of serum bile acids in amyotrophic lateral sclerosis","authors":"Ikjae Lee, Renu Nandakumar, Rebecca A. Haeusler","doi":"10.1002/lipd.12390","DOIUrl":"10.1002/lipd.12390","url":null,"abstract":"<p>Hydrophilic endogenous bile acids ursodeoxycholic acid (UDCA), tauroursodeoxycholic acid (TUDCA), and glucourosodeoxycholic acid (GUDCA) have suggested neuroprotective effects. We performed a case–control study to examine the association between ALS diagnosis and serum levels of bile acids. Sporadic and familial ALS patients, age- and sex-matched healthy controls, and presymptomatic gene carriers who donated blood samples were included. Non-fasted serum samples stored at −80°C were used for the analysis. Serum bile acid levels were measured by liquid chromatography-mass spectrometry (LC–MS). Concentrations of 15 bile acids were obtained, 5 non-conjugated and 10 conjugated, and compared between ALS versus control groups (presymptomatic gene carriers + healthy controls) using the Wilcoxon-Rank-Sum test. In total, 80 participants were included: 31 ALS (17 sporadic and 14 familial ALS); 49 controls (22 gene carriers, 27 healthy controls). The mean age was 50 years old and 50% were male. In the ALS group, 45% had familial disease with a pathogenic variant in <i>C9orf72</i> (29%), <i>TARDBP</i> (10%), <i>FUS</i> (3%), and <i>CHCHD10</i> (3%) genes. In the control group, 43% carried pathogenic variants: <i>C9orf72</i> (27%), <i>SOD1</i> (10%), and <i>FUS</i> (6%). The serum levels of UDCA, TUDCA, and GUDCA trended higher in the ALS group compared to controls (median 27 vs. 7 nM, 4 vs. 3 nM, 110 vs. 47 nM, <i>p</i>-values 0.04, 0.06, 0.04, respectively). No significant group differences were found in other bile acids serum levels. In conclusion, the serum level of UDCA, TUDCA, GUDCA trended higher in ALS patients compared to controls, and no evidence of deficiencies was found.</p>","PeriodicalId":18086,"journal":{"name":"Lipids","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2024-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139723148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Type 2 diabetes mellitus (T2DM) is a highly prevalent metabolic disorder. Insulin resistance and oxidative stress are associated with T2DM development. The hypothesis that patients with T2DM show excess accumulation of lipids, such as ceramides (Cers) and diacylglycerols (DAGs), in their skeletal muscles has been widely supported; however, detailed lipidomic data at the molecular species level are limited. Therefore, in this study, we aimed to investigate the in vitro dynamics of total lipids, including phospholipids (PLs), sphingolipids, and neutral lipids, in palmitic acid-induced insulin-resistant C2C12 skeletal muscle cells. Our data demonstrated that the profiles of not only Cers and DAGs but also those of PLs showed considerably differences after palmitate treatment. We found that PL synthesis reduced and PL degradation increased after palmitate treatment. These findings may aid in the development of treatments to ameliorate muscle dysfunction caused by lipid accumulation in muscles.
{"title":"A lipidomics approach reveals novel phospholipid changes in palmitate-treated C2C12 myotubes","authors":"Wakako Tawara, Mizuki Morisasa, Risa Mukai, Rei Suo, Shiro Itoi, Tsukasa Mori, Naoko Goto-Inoue","doi":"10.1002/lipd.12387","DOIUrl":"10.1002/lipd.12387","url":null,"abstract":"<p>Type 2 diabetes mellitus (T2DM) is a highly prevalent metabolic disorder. Insulin resistance and oxidative stress are associated with T2DM development. The hypothesis that patients with T2DM show excess accumulation of lipids, such as ceramides (Cers) and diacylglycerols (DAGs), in their skeletal muscles has been widely supported; however, detailed lipidomic data at the molecular species level are limited. Therefore, in this study, we aimed to investigate the in vitro dynamics of total lipids, including phospholipids (PLs), sphingolipids, and neutral lipids, in palmitic acid-induced insulin-resistant C2C12 skeletal muscle cells. Our data demonstrated that the profiles of not only Cers and DAGs but also those of PLs showed considerably differences after palmitate treatment. We found that PL synthesis reduced and PL degradation increased after palmitate treatment. These findings may aid in the development of treatments to ameliorate muscle dysfunction caused by lipid accumulation in muscles.</p>","PeriodicalId":18086,"journal":{"name":"Lipids","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139651023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
High-fat diets have been associated with colorectal cancer (CRC) risk, and the role of polyunsaturated fatty acids (PUFAs) has been reported to vary based on the length of PUFAs. We explored the association between dietary omega-6 and omega-3 PUFAs intake and CRC. We analyzed 865 CRC patients and 3206 controls from a case–control study of Iran (IROPICAN study). We used multivariate logistic regression models to calculate the odds ratios (OR) and 95% confidence intervals (CI) for the association between PUFAs intake and CRC risk. Our results showed that gamma-linolenic acid (18:3 n-6, GLA), arachidonic acid (20:4n-6, ARA), a-linolenic acid (Cis-18:3n-3, ALA), eicosapentaenoic acid (20:5n-3, EPA), docosahexaenoic acid (22:6n-3, DHA) consumption was not associated with the risk of CRC. However, the OR of linoleic acid (18: 2n-6, LA) intake was 1.47 (95% CI 1.01–2.14, p = 0.04) for proximal colon and that of docosapentaenoic acid (22:5n-3, DPA) intake was 1.33 (95% CI 1.05–1.69, p = 0.01) for rectum. This study indicates a high level of LA is associated with an increased risk of proximal colon cancer, and DPA intake was positively associated with rectum cancer risk. Furthermore, our study noted a high intake of n-6 (from vegetable oils) compared to n-3 PUFAs (from fish and seafood) in this population. Public awareness and government support is needed to increase fish and seafood production and consumption in Iran.
高脂肪饮食与结直肠癌(CRC)风险有关,而据报道,多不饱和脂肪酸(PUFAs)的作用因 PUFAs 的长度而异。我们探讨了膳食中欧米茄-6 和欧米茄-3 PUFAs 摄入量与 CRC 之间的关系。我们分析了伊朗一项病例对照研究(IROPICAN 研究)中的 865 例 CRC 患者和 3206 例对照者。我们使用多变量逻辑回归模型来计算 PUFAs 摄入量与 CRC 风险之间关系的几率比(OR)和 95% 的置信区间(CI)。结果显示,γ-亚麻酸(18:3 n-6,GLA)、花生四烯酸(20:4 n-6,ARA)、a-亚麻酸(顺式-18:3 n-3,ALA)、二十碳五烯酸(20:5 n-3,EPA)、二十二碳六烯酸(22:6 n-3,DHA)的摄入量与 CRC 风险无关。然而,亚油酸(18:2n-6,LA)摄入量的 OR 值在近端结肠为 1.47(95% CI 1.01-2.14,p = 0.04),摄入二十二碳五烯酸(22:5n-3,DPA)的 OR 值在直肠为 1.33(95% CI 1.05-1.69,p = 0.01)。这项研究表明,高水平的 LA 与近端结肠癌风险增加有关,而 DPA 摄入量与直肠癌风险呈正相关。此外,我们的研究还发现,与 n-3 PUFAs(来自鱼类和海产品)相比,该人群的 n-6(来自植物油)摄入量较高。伊朗需要提高公众意识和政府支持,以增加鱼类和海产品的生产和消费。
{"title":"Role of dietary intake of specific polyunsaturated fatty acids (PUFAs) on colorectal cancer risk in Iran","authors":"Monireh Sadat Seyyedsalehi, Maryam Hadji, Giulia Collatuzzo, Hamideh Rashidian, Bahareh Sasanfar, Inge Huybrechts, Veronique Chajes, Paolo Boffetta, Kazem Zendehdel","doi":"10.1002/lipd.12386","DOIUrl":"10.1002/lipd.12386","url":null,"abstract":"<p>High-fat diets have been associated with colorectal cancer (CRC) risk, and the role of polyunsaturated fatty acids (PUFAs) has been reported to vary based on the length of PUFAs. We explored the association between dietary omega-6 and omega-3 PUFAs intake and CRC. We analyzed 865 CRC patients and 3206 controls from a case–control study of Iran (IROPICAN study). We used multivariate logistic regression models to calculate the odds ratios (OR) and 95% confidence intervals (CI) for the association between PUFAs intake and CRC risk. Our results showed that gamma-linolenic acid (18:3 n-6, GLA), arachidonic acid (20:4n-6, ARA), a-linolenic acid (Cis-18:3n-3, ALA), eicosapentaenoic acid (20:5n-3, EPA), docosahexaenoic acid (22:6n-3, DHA) consumption was not associated with the risk of CRC. However, the OR of linoleic acid (18: 2n-6, LA) intake was 1.47 (95% CI 1.01–2.14, <i>p</i> = 0.04) for proximal colon and that of docosapentaenoic acid (22:5n-3, DPA) intake was 1.33 (95% CI 1.05–1.69, <i>p</i> = 0.01) for rectum. This study indicates a high level of LA is associated with an increased risk of proximal colon cancer, and DPA intake was positively associated with rectum cancer risk. Furthermore, our study noted a high intake of n-6 (from vegetable oils) compared to n-3 PUFAs (from fish and seafood) in this population. Public awareness and government support is needed to increase fish and seafood production and consumption in Iran.</p>","PeriodicalId":18086,"journal":{"name":"Lipids","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139575907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Recent studies have identified monocyte-to-high-density lipoprotein cholesterol ratio (MHR) as a simple marker of atherosclerosis. Abdominal aortic calcification (AAC) is a direct result of vascular atherosclerosis. Our study aims to investigate the association between MHR and the prevalent extensive AAC and assess the value of MHR for identifying prevalent extensive AAC. 2857 subjects (28.07%) from the cross-sectional National Health and Nutrition Examination Survey 2013–2014 were included in our study. AAC was detected through dual-energy x-ray absorptiometry and quantified by Kauppila score. Extensive AAC was identified in 153 (10.44% of 1465) females and 146 (10.49% of 1392) males. With the full adjustment, each SD increase of MHR resulted in an 87.3% additional risk for extensive AAC in females. When dividing into quartiles, the top quartile had a 3.472 times risk of prevalent extensive AAC than the bottom quartile. However, no significant association was observed in males. Furthermore, smooth curve fitting implicated that the significant association was linear in the whole range of MHR among females. Additionally, ROC demonstrated an improvement in the identification of extensive AAC only among females when introducing MHR into established risk factors of atherosclerosis (0.808 vs. 0.864, p < 0.001). Finally, category-free net reclassification index and integrated discrimination index also supported the improvement by MHR in females. Our study revealed a linear association between MHR and prevalent extensive AAC in females. Moreover, our results implicated the potential value of MHR to refine the identification of prevalent extensive AAC in females.
{"title":"Sex-specific association between monocyte to high-density lipoprotein cholesterol and extensive abdominal aortic calcification in humans","authors":"Yuyu Niu, Guifang Wang, Xianjun Feng, Hongyi Niu, Wenrui Shi, Yingxue Shen","doi":"10.1002/lipd.12385","DOIUrl":"10.1002/lipd.12385","url":null,"abstract":"<p>Recent studies have identified monocyte-to-high-density lipoprotein cholesterol ratio (MHR) as a simple marker of atherosclerosis. Abdominal aortic calcification (AAC) is a direct result of vascular atherosclerosis. Our study aims to investigate the association between MHR and the prevalent extensive AAC and assess the value of MHR for identifying prevalent extensive AAC. 2857 subjects (28.07%) from the cross-sectional National Health and Nutrition Examination Survey 2013–2014 were included in our study. AAC was detected through dual-energy x-ray absorptiometry and quantified by Kauppila score. Extensive AAC was identified in 153 (10.44% of 1465) females and 146 (10.49% of 1392) males. With the full adjustment, each SD increase of MHR resulted in an 87.3% additional risk for extensive AAC in females. When dividing into quartiles, the top quartile had a 3.472 times risk of prevalent extensive AAC than the bottom quartile. However, no significant association was observed in males. Furthermore, smooth curve fitting implicated that the significant association was linear in the whole range of MHR among females. Additionally, ROC demonstrated an improvement in the identification of extensive AAC only among females when introducing MHR into established risk factors of atherosclerosis (0.808 vs. 0.864, <i>p</i> < 0.001). Finally, category-free net reclassification index and integrated discrimination index also supported the improvement by MHR in females. Our study revealed a linear association between MHR and prevalent extensive AAC in females. Moreover, our results implicated the potential value of MHR to refine the identification of prevalent extensive AAC in females.</p>","PeriodicalId":18086,"journal":{"name":"Lipids","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139570693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Edgar Willibaldo Allebrandt Neto, Jadyellen Rondon e Silva, Stephanie Figueiredo Santos, Suélem Aparecida de França Lemes, Nair Honda Kawashita, Mayara Peron Pereira
The low-protein, high-carbohydrate (LPHC) diet administered to growing rats soon after weaning, for 15 days, promoted an increase in energy expenditure by uncoupling protein 1 (UCP1) in interscapular brown adipose tissue, and also due to the occurrence of the browning process in the perirenal white adipose tissue (periWAT). However, we believe that inguinal white adipose tissue (ingWAT) may also contribute to energy expenditure through other mechanisms. Therefore, the aim of this work is to investigate the presence of the futile creatine cycle, and the origin of lipids in ingWAT, since that tissue showed an increase in the lipids content in rats submitted to the LPHC diet for 15 days. We observed increases in creatine kinase and alkaline phosphatase activity in ingWAT, of the LPHC animals. The mitochondrial Nicotinamide adenine dinucleotide reduced/nicotinamide adenine dinucleotide oxidized ratio is lower in ingWAT of LPHC animals. In the LPHC animals treated with β-guanidinopropionic acid, the extracellular uptake of creatine in ingWAT was lower, as was the rectal temperature. Regarding lipid metabolism, we observed that in ingWAT, lipolysis in vitro when stimulated with noradrenaline is lower, and there were no changes in baseline levels. In addition, increases in the activity of enzymes were also observed: malic, glucose-6-phosphate dehydrogenase, and ATP-citrate lyase, in addition to an increase in the PPARγ content. The results show the occurrence of the futile creatine cycle in ingWAT, and that the increase in the relative mass may be due to an increase in de novo fatty acid synthesis.
{"title":"The futile creatine cycle and the synthesis of fatty acids in inguinal white adipose tissue from growing rats, submitted to a hypoprotein-hyperglycidic diet for 15 days","authors":"Edgar Willibaldo Allebrandt Neto, Jadyellen Rondon e Silva, Stephanie Figueiredo Santos, Suélem Aparecida de França Lemes, Nair Honda Kawashita, Mayara Peron Pereira","doi":"10.1002/lipd.12384","DOIUrl":"10.1002/lipd.12384","url":null,"abstract":"<p>The low-protein, high-carbohydrate (LPHC) diet administered to growing rats soon after weaning, for 15 days, promoted an increase in energy expenditure by uncoupling protein 1 (UCP1) in interscapular brown adipose tissue, and also due to the occurrence of the browning process in the perirenal white adipose tissue (periWAT). However, we believe that inguinal white adipose tissue (ingWAT) may also contribute to energy expenditure through other mechanisms. Therefore, the aim of this work is to investigate the presence of the futile creatine cycle, and the origin of lipids in ingWAT, since that tissue showed an increase in the lipids content in rats submitted to the LPHC diet for 15 days. We observed increases in creatine kinase and alkaline phosphatase activity in ingWAT, of the LPHC animals. The mitochondrial Nicotinamide adenine dinucleotide reduced/nicotinamide adenine dinucleotide oxidized ratio is lower in ingWAT of LPHC animals. In the LPHC animals treated with β-guanidinopropionic acid, the extracellular uptake of creatine in ingWAT was lower, as was the rectal temperature. Regarding lipid metabolism, we observed that in ingWAT, lipolysis in vitro when stimulated with noradrenaline is lower, and there were no changes in baseline levels. In addition, increases in the activity of enzymes were also observed: malic, glucose-6-phosphate dehydrogenase, and ATP-citrate lyase, in addition to an increase in the PPARγ content. The results show the occurrence of the futile creatine cycle in ingWAT, and that the increase in the relative mass may be due to an increase in de novo fatty acid synthesis.</p>","PeriodicalId":18086,"journal":{"name":"Lipids","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139466718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Amber B. Courville, Sharon Majchrzak-Hong, Shanna Yang, Sara Turner, Breanne Wilhite, Katherine Ness Shipley, Yvonne Horneffer, Anthony F. Domenichiello, Melanie Schwandt, Roy G. Cutler, Kong Y. Chen, Joseph R. Hibbeln, Christopher E. Ramsden
The linoleic acid (LA)-arachidonic acid (ARA)-inflammatory axis suggests dietary LA lowering benefits health because it lowers ARA and ARA-derived endocannabinoids (ECB). Dietary LA reduction increases concentrations of omega-3 eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) and DHA derived ECB. The aim of this study was to examine targeted reduction of dietary LA, with and without EPA and DHA, on plasma EPA and DHA and ECB (2-arachidonoyl glycerol [2-AG], anandamide [AEA], and docosahexaenoyl ethanolamide [DHA-EA]). Healthy, pre-menopausal women (n = 62, BMI 30 ± 3 kg/m2, age 35 ± 7 years; mean ± SD) were randomized to three 12-week controlled diets: (1) high LA, low omega-3 EPA and DHA (H6L3); (2) low LA, low omega-3 EPA and DHA (L6L3); or (3) low LA, high omega-3 EPA and DHA (L6H3). Baseline plasma fatty acids and ECB were similar between diets. Starting at 4 weeks, L6L3 and L6H3 lowered plasma LA compared to H6L3 (p < 0.001). While plasma ARA changed from baseline by 8% in L6L3 and −8% in L6H3, there were no group differences. After 4 weeks, plasma EPA and DHA increased from baseline in women on the L6H3 diet (ps < 0.001) and were different than the H6L3 and L6L3 diets. No differences were found between diets for AEA or 2-AG, however, in L6L3 and L6H3, AEA increased by 14% (ps < 0.02). L6H3 resulted in 35% higher DHA-EA (p = 0.013) whereas no changes were seen with the other diets. Lowering dietary LA did not result in the expected changes in fatty acids associated with the LA-ARA inflammatory axis in women with overweight and obesity.
{"title":"Dietary linoleic acid lowering alone does not lower arachidonic acid or endocannabinoids among women with overweight and obesity: A randomized, controlled trial","authors":"Amber B. Courville, Sharon Majchrzak-Hong, Shanna Yang, Sara Turner, Breanne Wilhite, Katherine Ness Shipley, Yvonne Horneffer, Anthony F. Domenichiello, Melanie Schwandt, Roy G. Cutler, Kong Y. Chen, Joseph R. Hibbeln, Christopher E. Ramsden","doi":"10.1002/lipd.12382","DOIUrl":"https://doi.org/10.1002/lipd.12382","url":null,"abstract":"<p>The linoleic acid (LA)-arachidonic acid (ARA)-inflammatory axis suggests dietary LA lowering benefits health because it lowers ARA and ARA-derived endocannabinoids (ECB). Dietary LA reduction increases concentrations of omega-3 eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) and DHA derived ECB. The aim of this study was to examine targeted reduction of dietary LA, with and without EPA and DHA, on plasma EPA and DHA and ECB (2-arachidonoyl glycerol [2-AG], anandamide [AEA], and docosahexaenoyl ethanolamide [DHA-EA]). Healthy, pre-menopausal women (<i>n</i> = 62, BMI 30 ± 3 kg/m<sup>2</sup>, age 35 ± 7 years; mean ± SD) were randomized to three 12-week controlled diets: (1) high LA, low omega-3 EPA and DHA (H6L3); (2) low LA, low omega-3 EPA and DHA (L6L3); or (3) low LA, high omega-3 EPA and DHA (L6H3). Baseline plasma fatty acids and ECB were similar between diets. Starting at 4 weeks, L6L3 and L6H3 lowered plasma LA compared to H6L3 (<i>p</i> < 0.001). While plasma ARA changed from baseline by 8% in L6L3 and −8% in L6H3, there were no group differences. After 4 weeks, plasma EPA and DHA increased from baseline in women on the L6H3 diet (<i>p</i>s < 0.001) and were different than the H6L3 and L6L3 diets. No differences were found between diets for AEA or 2-AG, however, in L6L3 and L6H3, AEA increased by 14% (<i>p</i>s <i><</i> 0.02). L6H3 resulted in 35% higher DHA-EA (<i>p</i> = 0.013) whereas no changes were seen with the other diets. Lowering dietary LA did not result in the expected changes in fatty acids associated with the LA-ARA inflammatory axis in women with overweight and obesity.</p>","PeriodicalId":18086,"journal":{"name":"Lipids","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2023-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138678962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Adrian Arendowski, Ewelina Sibińska, Wioletta Miśta, Piotr Fijałkowski, Michał Złoch, Dorota Gabryś, Paweł Pomastowski
Lipids are one of the cell components therefore it is important to be able to accurately assess them. One of the analytical techniques used to study lipid profiles is matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI TOF MS). The present study attempted to select optimal conditions for sample preparation and MALDI MS analysis of bacterial lipidome in both positive and negative ion modes using different extraction protocols—Folch, Matyash, and Bligh & Dyer, solvents used to apply samples, and matrices such as 9-aminoacridine (9-AA), α-cyano-4-hydroxycinnamic acid (CHCA), 2,5-dihydroxybenzoic acid (DHB), 2-mercaptobenzothiazole (MBT), and 2,4,6-trihydroxyacetophenone (THAP). The obtained results allowed concluding that DHB or CHCA matrices are suitable for lipid analysis in the positive mode, and in the negative mode THAP or 9-AA. The most appropriate protocol for extracting lipids from bacterial cells was the Bligh & Dyer method in both ionization modes. The use of the solvent TA30, which was a mixture of acetonitrile and 0.1% trifluoroacetic acid in water, provided on the spectra a significant number of signals from lipids in all groups analyzed, such as fatty acyls, glycerolipids, and glycerophospholipids.
{"title":"Study of sample preparation influence on bacterial lipids profile in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry","authors":"Adrian Arendowski, Ewelina Sibińska, Wioletta Miśta, Piotr Fijałkowski, Michał Złoch, Dorota Gabryś, Paweł Pomastowski","doi":"10.1002/lipd.12383","DOIUrl":"10.1002/lipd.12383","url":null,"abstract":"<p>Lipids are one of the cell components therefore it is important to be able to accurately assess them. One of the analytical techniques used to study lipid profiles is matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI TOF MS). The present study attempted to select optimal conditions for sample preparation and MALDI MS analysis of bacterial lipidome in both positive and negative ion modes using different extraction protocols—Folch, Matyash, and Bligh & Dyer, solvents used to apply samples, and matrices such as 9-aminoacridine (9-AA), α-cyano-4-hydroxycinnamic acid (CHCA), 2,5-dihydroxybenzoic acid (DHB), 2-mercaptobenzothiazole (MBT), and 2,4,6-trihydroxyacetophenone (THAP). The obtained results allowed concluding that DHB or CHCA matrices are suitable for lipid analysis in the positive mode, and in the negative mode THAP or 9-AA. The most appropriate protocol for extracting lipids from bacterial cells was the Bligh & Dyer method in both ionization modes. The use of the solvent TA30, which was a mixture of acetonitrile and 0.1% trifluoroacetic acid in water, provided on the spectra a significant number of signals from lipids in all groups analyzed, such as fatty acyls, glycerolipids, and glycerophospholipids.</p>","PeriodicalId":18086,"journal":{"name":"Lipids","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2023-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138552585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Giulia Cisbani, Raphaël Chouinard-Watkins, Mackenzie E. Smith, Arezou Malekanian, Rodrigo Valenzuela, Adam H. Metherel, Richard P. Bazinet
Consumption of a Western diet (WD) is known to increase the risk of obesity. Short or medium chain fatty acids influence energy metabolism, and triacetin, a synthetic short chain triacylglyceride, has been shown to lower body fat under normal conditions. This study aimed to investigate if triacetin as part of a WD modifies rat weight and body fat. Male rats were fed a control diet or WD for 8 weeks. At week 8, rats in the WD group were maintained on a WD diet or switched to a WD diet containing 30% energy from medium-chain triacylglyceride (WD-MCT) or triacetin (WD-T) for another 8 weeks. At week 16, rats were euthanized and liver, adipose and blood were collected. Tissue fatty acids (FAs) were quantified by gas chromatography (GC) and hepatic FAs were measured by GC-combustion-isotope ratio mass spectrometry for δ13C-palmitic acid (PAM)—a novel marker of de novo lipogenesis (DNL). Rats fed WD-T had a body weight not statistically different to the control group, and gained less body weight than rats fed WD alone. Furthermore, WD-T fed rats had a lower fat mass, and lower total liver and plasma FAs compared to the WD group. Rats fed WD-T did not differ from WD in blood ketone or glucose levels, however, had a significantly lower hepatic δ13C-PAM value than WD fed rats; suggestive of lower DNL. In summary, we show that triacetin has the potential to blunt weight gain and adipose tissue accumulation in a rodent model of obesity, possibly due to a decrease in DNL.
众所周知,西方饮食(WD)会增加肥胖的风险。短链或中链脂肪酸影响能量代谢,而合成的短链甘油三酯三乙酸酯已被证明能在正常情况下降低体脂。本研究旨在探讨三乙酸乙酯作为WD的一部分是否会改变大鼠体重和体脂。雄性大鼠分别饲喂对照日粮或WD 8周。在第8周,WD组大鼠继续维持WD饮食或切换到含有30%中链甘油三酯(WD- mct)或三乙酸酯(WD- t)能量的WD饮食,再持续8周。第16周处死大鼠,取肝脏、脂肪和血液。组织脂肪酸(FAs)采用气相色谱(GC)定量,肝脏脂肪酸(FAs)采用气相色谱-燃烧-同位素比值质谱法测定δ13 c -棕榈酸(PAM)-一种新的de novo lipogenesis (DNL)标志物。饲喂WD- t的大鼠体重与对照组无统计学差异,且体重增长小于单独饲喂WD的大鼠。此外,与WD组相比,WD- t喂养的大鼠脂肪质量更低,肝脏和血浆总FAs也更低。大鼠的血酮和葡萄糖水平与对照组无显著差异,但肝脏δ13 C-PAM值明显低于对照组;提示下DNL。总之,我们表明,在肥胖的啮齿动物模型中,三乙酸乙酯具有钝化体重增加和脂肪组织积累的潜力,可能是由于DNL的减少。
{"title":"Dietary triacetin, but not medium chain triacylglycerides, blunts weight gain in diet-induced rat model of obesity","authors":"Giulia Cisbani, Raphaël Chouinard-Watkins, Mackenzie E. Smith, Arezou Malekanian, Rodrigo Valenzuela, Adam H. Metherel, Richard P. Bazinet","doi":"10.1002/lipd.12381","DOIUrl":"10.1002/lipd.12381","url":null,"abstract":"<p>Consumption of a Western diet (WD) is known to increase the risk of obesity. Short or medium chain fatty acids influence energy metabolism, and triacetin, a synthetic short chain triacylglyceride, has been shown to lower body fat under normal conditions. This study aimed to investigate if triacetin as part of a WD modifies rat weight and body fat. Male rats were fed a control diet or WD for 8 weeks. At week 8, rats in the WD group were maintained on a WD diet or switched to a WD diet containing 30% energy from medium-chain triacylglyceride (WD-MCT) or triacetin (WD-T) for another 8 weeks. At week 16, rats were euthanized and liver, adipose and blood were collected. Tissue fatty acids (FAs) were quantified by gas chromatography (GC) and hepatic FAs were measured by GC-combustion-isotope ratio mass spectrometry for δ<sup>13</sup>C-palmitic acid (PAM)—a novel marker of de novo lipogenesis (DNL). Rats fed WD-T had a body weight not statistically different to the control group, and gained less body weight than rats fed WD alone. Furthermore, WD-T fed rats had a lower fat mass, and lower total liver and plasma FAs compared to the WD group. Rats fed WD-T did not differ from WD in blood ketone or glucose levels, however, had a significantly lower hepatic δ<sup>13</sup>C-PAM value than WD fed rats; suggestive of lower DNL. In summary, we show that triacetin has the potential to blunt weight gain and adipose tissue accumulation in a rodent model of obesity, possibly due to a decrease in DNL.</p>","PeriodicalId":18086,"journal":{"name":"Lipids","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2023-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://aocs.onlinelibrary.wiley.com/doi/epdf/10.1002/lipd.12381","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138299515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Liver inflammation has become increasingly prevalent in recent years, leading to the development of diseases like hepatitis, alcoholic liver disease, and fatty liver disease. One factor that has been linked to liver inflammation is increased levels of lipopolysaccharides (LPS), which can be caused by poor diets and sedentary lifestyles that contribute to liver inflammation. There is promising research on a new class of lipids called fatty acid esters of hydroxy fatty acids (FAHFAs), which have been shown to potentiate insulin release and exert an anti-inflammatory effect. Specifically, one type of FAHFA called 9-POHSA (palmitoleic acid ester of 9-hydroxy stearic acid) has been studied for its potential to attenuate inflammation-related indexes induced by LPS in hepatocytes, which play a critical role in the progression of liver inflammation. This study found that following LPS treatment, tumor necrosis factor- α, interleukin-6, and connective tissue growth factor (CTGF) were upregulated and increased cell migration, but 9-POHSA pre-treatment attenuated the upregulation of these markers and prevented cell migration induced by LPS. Using flowcytometry analysis, intracellular reactive oxygen species (ROS) was found to be responsible for CTGF upregulation. In addition, the effects of 9-POHSA were likely associated with its inhibition of the activation of the NF-kB. These results suggest that 9-POHSA has potential as a therapy for liver inflammation and fibrosis by attenuating inflammation-related indexes induced by LPS in hepatocytes. This study provides important insight into the mechanisms of liver inflammation and the potential for new treatments to address liver diseases.
{"title":"9-POHSA prevents NF-kB activation and ameliorates LPS-induced inflammation in rat hepatocytes","authors":"Jiro Hasegawa Situmorang, Ming-Cheng Chen, Wei-Wen Kuo, Shinn-Zong Lin, Cheng-Yen Shih, Pi-Yu Lin, Ching-Hui Loh, Chih-Yang Huang","doi":"10.1002/lipd.12380","DOIUrl":"10.1002/lipd.12380","url":null,"abstract":"<p>Liver inflammation has become increasingly prevalent in recent years, leading to the development of diseases like hepatitis, alcoholic liver disease, and fatty liver disease. One factor that has been linked to liver inflammation is increased levels of lipopolysaccharides (LPS), which can be caused by poor diets and sedentary lifestyles that contribute to liver inflammation. There is promising research on a new class of lipids called fatty acid esters of hydroxy fatty acids (FAHFAs), which have been shown to potentiate insulin release and exert an anti-inflammatory effect. Specifically, one type of FAHFA called 9-POHSA (palmitoleic acid ester of 9-hydroxy stearic acid) has been studied for its potential to attenuate inflammation-related indexes induced by LPS in hepatocytes, which play a critical role in the progression of liver inflammation. This study found that following LPS treatment, tumor necrosis factor- α, interleukin-6, and connective tissue growth factor (CTGF) were upregulated and increased cell migration, but 9-POHSA pre-treatment attenuated the upregulation of these markers and prevented cell migration induced by LPS. Using flowcytometry analysis, intracellular reactive oxygen species (ROS) was found to be responsible for CTGF upregulation. In addition, the effects of 9-POHSA were likely associated with its inhibition of the activation of the NF-kB. These results suggest that 9-POHSA has potential as a therapy for liver inflammation and fibrosis by attenuating inflammation-related indexes induced by LPS in hepatocytes. This study provides important insight into the mechanisms of liver inflammation and the potential for new treatments to address liver diseases.</p>","PeriodicalId":18086,"journal":{"name":"Lipids","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2023-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10206979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Shakeri, H, Hadaegh, H, Abedi, F, Tajabadi-Ebrahimi, M, Mazroii, N, Ghandi, Y and Asemi, Z. Consumption of synbiotic bread decreases triacylglycerol and VLDL levels while increasing HDL levels in serum from patients with type-2 diabetes. Lipids. 2014;49:695–701. https://doi.org/10.1007/s11745-014-3901-z.
The journal is aware of a number of issues raised regarding the integrity of research published by Zatolleh Asemi and colleagues (Grey et al., 2020). An investigation into these concerns has been conducted by The National Committee for Ethics in Biomedical Research Iran, in coordination with Kashan University of Medical Sciences (KAUMS). The Editor has independently considered the concerns reported for this article and did not find evidence of questionable research practices at this time. The following corrections have therefore been made.
In the above-referenced published article, the study was reported to have taken place between October 2013 and December 2013. This is an apparent error in the preparation of the manuscript. The correct timing of the study was between December 2013 and January 2014. In the published article Figure 1 and Tables 1, 2, and 3 state that the number of participants for which samples were analyzed was 26. This is a systematic typographical error; the correct number is 24 participants. The authors neglected to acknowledge the contribution to the study of Drs. Maryam Tajabadi-Ebrahimi and Haleh Hadaegh. The correct version of the acknowledgment should therefore be: Acknowledgments: The present study was supported by a Grant (No. 92107) from the Vice-Chancellor for Research, KAUMS, Kashan, Iran. The authors would like to thank the staff of Gholabchi Clinic (Kashan, Iran) for their assistance in this project. We are grateful to the Research and Development Department of Sahar Bread Company, Tehran, Iran that provided probiotic and synbiotic products for the present study. Furthermore, we are grateful to the Research and Development Department of Tak Gene Zist Company, Tehran, Iran that provided Lactobacillus sporogenes for this study. The authors wish to acknowledge the contributions of Drs. Maryam Tajabadi-Ebrahimi and Haleh Hadaegh who conducted the analysis of the blood samples. The authors apologize for any inconvenience these errors and oversights may have caused.
Shakeri, H, Hadaegh, H, Abedi, F, Tajabadi-Ebrahimi, M, Mazroii, N, Ghandi, Y和Asemi, Z.食用合成面包可降低2型糖尿病患者血清中甘油三酯和VLDL水平,同时提高高密度脂蛋白水平。脂质。2014;49:695 - 701。https://doi.org/10.1007/s11745-014-3901-z.The期刊注意到Zatolleh Asemi及其同事发表的研究的完整性问题(Grey et al., 2020)。伊朗生物医学研究伦理全国委员会与卡尚医学科学大学(KAUMS)协调,对这些问题进行了调查。编辑独立考虑了本文所报道的问题,目前没有发现有问题的研究实践的证据。因此作出下列更正。在上述发表的文章中,研究报告发生在2013年10月至2013年12月之间。这是准备手稿时的一个明显错误。研究的正确时间是2013年12月至2014年1月。在发表的文章中,图1和表1、表2和表3表明,分析样本的参与者数量为26。这是一个系统性的印刷错误;正确的数字是24人。作者忽略了承认博士的研究贡献。Maryam Tajabadi-Ebrahimi和Haleh Hadaegh。因此,致谢的正确版本应该是:致谢:本研究得到了伊朗卡尚KAUMS研究副校长的资助(编号92107)。作者要感谢Gholabchi诊所(伊朗卡尚)的工作人员在这个项目中的帮助。我们感谢伊朗德黑兰萨哈尔面包公司研发部为本研究提供益生菌和合成菌产品。此外,我们感谢伊朗德黑兰Tak Gene Zist公司的研发部门为本研究提供了产孢乳杆菌。作者希望感谢博士的贡献。Maryam Tajabadi-Ebrahimi和Haleh Hadaegh对血液样本进行了分析。作者对这些错误和疏忽可能造成的任何不便表示歉意。
{"title":"Correction to “Consumption of synbiotic bread decreases triacylglycerol and VLDL levels while increasing HDL levels in serum from patients with type-2 diabetes”","authors":"","doi":"10.1002/lipd.12379","DOIUrl":"10.1002/lipd.12379","url":null,"abstract":"<p>Shakeri, H, Hadaegh, H, Abedi, F, Tajabadi-Ebrahimi, M, Mazroii, N, Ghandi, Y and Asemi, Z. Consumption of synbiotic bread decreases triacylglycerol and VLDL levels while increasing HDL levels in serum from patients with type-2 diabetes. Lipids. 2014;49:695–701. https://doi.org/10.1007/s11745-014-3901-z.</p><p>The journal is aware of a number of issues raised regarding the integrity of research published by Zatolleh Asemi and colleagues (Grey et al., <span>2020</span>). An investigation into these concerns has been conducted by The National Committee for Ethics in Biomedical Research Iran, in coordination with Kashan University of Medical Sciences (KAUMS). The Editor has independently considered the concerns reported for this article and did not find evidence of questionable research practices at this time. The following corrections have therefore been made.</p><p>In the above-referenced published article, the study was reported to have taken place between October 2013 and December 2013. This is an apparent error in the preparation of the manuscript. The correct timing of the study was between December 2013 and January 2014. In the published article Figure 1 and Tables 1, 2, and 3 state that the number of participants for which samples were analyzed was 26. This is a systematic typographical error; the correct number is 24 participants. The authors neglected to acknowledge the contribution to the study of Drs. Maryam Tajabadi-Ebrahimi and Haleh Hadaegh. The correct version of the acknowledgment should therefore be: Acknowledgments: The present study was supported by a Grant (No. 92107) from the Vice-Chancellor for Research, KAUMS, Kashan, Iran. The authors would like to thank the staff of Gholabchi Clinic (Kashan, Iran) for their assistance in this project. We are grateful to the Research and Development Department of Sahar Bread Company, Tehran, Iran that provided probiotic and synbiotic products for the present study. Furthermore, we are grateful to the Research and Development Department of Tak Gene Zist Company, Tehran, Iran that provided Lactobacillus sporogenes for this study. The authors wish to acknowledge the contributions of Drs. Maryam Tajabadi-Ebrahimi and Haleh Hadaegh who conducted the analysis of the blood samples. The authors apologize for any inconvenience these errors and oversights may have caused.</p>","PeriodicalId":18086,"journal":{"name":"Lipids","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2023-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/lipd.12379","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10194916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}